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  • 1
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton -- Visualizing In Vitro Trafficking -- Interaction of the Glycocalyx with the Actin Cytoskeleton -- Cytoskeleton Dynamics and Binding Factors -- Principles of Proteomic Approaches to the Cytoskeleton -- Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus -- Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes -- The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases -- Neurofilaments: Properties, Functions, and Regulation -- From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics -- Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton -- Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact -- Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis -- Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System -- Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
    Abstract: The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered.℗ The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system.℗ Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results. ℗ Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton
    Pages: XIV, 359 p. 52 illus., 31 illus. in color. : digital.
    ISBN: 9781627032667
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  • 2
    Keywords: Medicine ; Human Physiology ; Neurosciences ; Biomedicine ; Neurosciences ; Human Physiology ; Springer eBooks
    Description / Table of Contents: Optogenetic Stimulation of Neurons in the Brainstem of Freely Behaving Rats and Mice -- Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex responses in the In Situ Arterially-Perfused Preparation -- Excitatory Responses to Microinjection of Glutamate Depend on Dose not Volume: A Meta-Analysis of Studies in Rat RVLM -- Acute Activation and Inhibition of the Sympathetic Baroreceptor Reflex -- Pharmacological Dissection of G Protein-Mediated Second Messenger Cascades in Neurons -- In Vivo Manipulation of Intracellular Signalling Pathways -- Identification of Spinally Projecting Neurons in the Rostral Ventrolateral Medulla In Vivo -- Microionotophoretic Study of Individual Neurons During Intracellular Recording -- Neurobiotin Electroporation for Combined Structural and Functional Analysis of Neurons in Developing Mouse Brain Slices -- Juxtacellular Neuronal Labelling, Physiological Characterization, and Phenotypic Identification of Single Neurons In Vivo -- Analysis of Sympathetic Nerve Activity Analysis of Sympathetic Nerve Activity -- Insight into Autonomic Nervous System Control of Heart Rate in the Rat Using Analysis of Heart Rate Variability and Baroreflex Sensitivity -- Neurophysiological Recording of the Compound Muscle Action Potential for Motor Unit Number Estimation in Mice -- Animal Models of Neuropathic Pain Due to Nerve Injury -- Detection of Sensitized Nerve Responses: Dorsal Root Reflexes, Live Cell Calcium, and ROS Imaging -- Visual Evoked Potential Recording in Rodents -- The Visual Evoked Potential in Humans -- Improving Motor Activation Patterns after Stroke with Wii-Based Movement Therapy
    Abstract: Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools.℗ In Stimulation and Inhibition of Neurons, experts in the field provide an overview of modern methods for generating lesions as well as for stimulating and inhibiting neural pathways.℗ Many new techniques such as optogenetics and the use of the in situ perfused preparation are examined, while, in other sections, the use and validity of more well-known approaches are reassessed. Written for the Neuromethods series, chapters examine their respective topics thoroughly and include the kind of detail and implementation advice that ensures successful results in the laboratory. ℗ Authoritative and cutting-edge, Stimulation and Inhibition of Neurons serves as an ideal guide for researchers seeking to gain further knowledge of the complex functions of the brain
    Pages: XII, 319 p. 73 illus., 15 illus. in color. : digital.
    ISBN: 9781627032339
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  • 3
    Keywords: Neurosciences ; Cell Culture ; Neurosciences ; Cell Culture ; Springer eBooks
    Description / Table of Contents: In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model -- In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models -- Oxidative Stress Signatures in Human Stem Cell Derived Neurons -- Glial Reactivity in Response to Neurotoxins: Relevance and Methods -- Neuron-Glia Interaction Studied with In Vitro Co-Culture -- Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria -- Epigenetic Changes in Cultured Neurons and Astrocytes -- The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation -- Zebrafish as a Tool to Assess Developmental Neurotoxicity -- Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies -- Electrophysiological Neuromethodologies -- A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies -- Transporter Studies: Brain Punching Technique -- MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders -- Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes
    Abstract: This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field
    Pages: XIII, 302 p. 57 illus., 47 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992287
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  • 4
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume describes different computational methods encompassing ligand-based approaches (QSAR, pharmcophore), structure-based approaches (homology modeling, docking, molecular dynamics simulation), and combined approaches (virtual screening) with applications in anti-Alzheimer drug design. Different background topics like molecular etiologies of Alzheimer’s disease, targets for new drug development, and different cheminformatic modeling strategies are covered for completeness. Special topics like multi-target drug development, natural products, protein misfolding, and nanomaterials are also included in connection with computational modeling of anti-Alzheimer drug development. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Computational Modeling of Drugs Against Alzheimer’s Disease is a valuable resource for learning about the latest computational techniques used to study this disease
    Pages: XVII, 645 p. 222 illus., 158 illus. in color. : online resource.
    ISBN: 9781493974047
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  • 5
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons
    Pages: XVI, 382 p. 90 illus., 67 illus. in color. : online resource.
    ISBN: 9781493972807
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  • 6
    Keywords: Neurosciences ; Laboratory animals ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Fluid Percussion Injury Rodent Model in Preclinical Research on Traumatic Brain Injury -- Focal and Restricted Traumatic Injury Models in the Rodent Brain—Limitations, Possibilities, and Challenges -- A Model for Research on Penetrating Traumatic Brain Injuries -- A Sagittal Plane Rotational Injury Rodent Model for Research on Traumatic Brain Injuries -- Experimental Models of Blast-Induced Neurotrauma -- Utilization of Shock Tubes in Blast Injury Research -- Introduction to Blast in the Context of Blast Induced TBI -- The Clemedson Blast Tube -- Traumatic Peripheral Nerve Injuries – Experimental Models for Repair and Reconstruction -- CNS Regeneration in Nerve Grafts - Practical Aspects of Complete Thoracic Spinal Cord Injury in Rodents -- Experimental Nerve Plexus Root Avulsion and Spinal Cord Implantation -- Surgical Replantation of Avulsed Lumbosacral Ventral Roots and Urodynamic Studies in a Rhesus Macaque (Macaca mulatta) Model of Cauda Equina/Conus Medullaris Injury and Repair -- Secondary Insults in Experimental Traumatic Brain Injury – The Addition of Hypoxia -- A Translational Model of Traumatic Brain Injury: Sheep Impact Acceleration -- In Vitro Trauma – Description of a Cavitation Inducing Injury Model -- Clinically Relevant Outcome Measures for Experimental Traumatic Brain Injury (TBI) Studies -- Concluding Remarks
    Abstract: This volume looks into the need for a variety of experimental models for research on traumatic brain injury (TBI) and peripheral nervous system. It also describes a number of experimental models, such as mechanical devices, that have been developed to model neurotrauma in animal experiments. The overall aim of this book is to explore the variety of models and how they are used in current research. The chapters in this book are organized in four sections and talk about animal models for TBI; animal models for spinal cord or nerve injury; and translational aspects, secondary injuries, in vitro studies, and evaluation of large data sets. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Animal Models of Neurotrauma is a valuable resource for researchers interested in expanding their knowledge and research in this developing field
    Pages: XIII, 302 p. 93 illus., 63 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493997114
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  • 7
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: In Vivo Whole-Cell Recordings -- Juxtasomal Loose-Patch Recordings in Awake, Head-Fixed Rats to Study the Link between Structure and Function of Individual Neurons -- Studying Sodium Channel Gating In Heterologous Expression Systems -- Elucidating the Link between Structure and Function of Ion Channels and Transporters with Voltage-Clamp and Patch-Clamp Fluorometry -- Dendrites: Recording from Fine Neuronal Structures Using Patch-Clamp and Imaging Techniques -- Patch-clamp Recording from Myelinated Central Axons -- Analysis of Transsynaptic Attentional Neuronal℗ Circuits with Octuple Patch-Clamp Recordings -- Optogenetic Dissection of the Striatal Microcircuitry -- Paired Recordings from Synaptically Coupled Neurones in Acute Neocortical Slices -- Extracting Quantal Properties of Transmission at Central Synapses -- Acousto-optical Scanning-Based High Speed 3D Two-Photon Imaging In Vivo -- Intracellular Voltage-Sensitive Dyes for Studying Dendritic Excitability and Synaptic Integration -- Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels -- Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches -- Creating and Constraining Compartmental Models of Neurons Using Experimental Data
    Abstract: This volume presents current adaptations of the patch-clamp technique to neuroscience. Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical,℗ Advanced℗ Patch-Clamp Analysis for Neuroscientists aims℗ to ensure successful results in the further study of this vital field
    Pages: XII, 350 p. 81 illus., 28 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493934119
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  • 8
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Determination of CO2 Production in Subcellular Preparations like Synaptosomes and Isolated Mitochondria Using 14C-Labeled Substrates and Radioactive CO2 Measurements -- Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes -- Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells -- Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS) -- Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro -- Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose -- Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes -- RNA Interference as a Tool to Selectively Down-Modulate Protein Function -- Localizing and Quantifying Metabolites In Situ with Luminometry: Induced Metabolic Bioluminescence Imaging (imBI) -- A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology -- Integrated Measurements of Electrical Activity, Oxygen Tension, Blood Flow, and Ca2+-Signaling in Rodents In Vivo -- Measuring Cerebral Hemodynamics and Energy Metabolism by Near-Infrared Spectroscopy -- Compartmental Analysis of Metabolism by 13C Magnetic Resonance Spectroscopy -- Positron Emission Tomography of Brain Glucose Metabolism with [18F]Fluorodeoxyglucose in Humans
    Abstract: Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab. ℗ Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field
    Pages: XIV, 368 p. 97 illus., 52 illus. in color. : online resource.
    ISBN: 9781493910595
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  • 9
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: A Survey of Current Neuroanatomical Tracing Techniques.-℗ Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry -- Retrograde Tract-Tracing ́€˜Pluś€™: Adding Extra Value to Retrogradely-Traced Neurons -- Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice -- Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics -- Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes -- Optogenetic Dissection of Neural Circuit Function in Behaving Animals -- Remote Control of Neural Activity Using Chemical Genetics -- Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits -- Engineered Rabies Virus for Transsynaptic Circuit Tracing -- Genetic Labeling of Synapses -- Genetic Pathways to Circuit Understanding in Drosophila
    Abstract: This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study
    Pages: XII, 278 p. 52 illus., 47 illus. in color. : online resource.
    ISBN: 9781493919635
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  • 10
    Unknown
    New York, NY : Springer
    Keywords: Medicine ; Neurosciences ; Neurochemistry ; Biomedicine ; Neurosciences ; Neurochemistry ; Springer eBooks
    Description / Table of Contents: Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene -- Characterization of D1 Dopamine Receptor Post-Transcriptional Regulation -- Computational Approaches in the Structure-Function Studies of Dopamine Receptors -- Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor -- Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors -- Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors -- Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods -- Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation -- Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors -- MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues -- Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats -- Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors -- Dopamine Receptors in Human Embryonic Stem Cell Differentiation -- Calcium and PLC Signaling Through Dopamine Receptors -- Intracellular Trafficking Assays for Dopamine D2-Like Receptors -- Study of Crosstalk Between Dopamine Receptors and Ion Channels -- Study of Dopamine Receptor and Dopamine Transporter Networks in Mice -- Optogenetic Regulation of Dopamine Receptor-Expressing Neurons -- Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property -- Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography
    Abstract: To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful. Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery
    Pages: XIV, 379 p. 63 illus., 26 illus. in color. : online resource.
    ISBN: 9781493921966
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