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  • 1
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Simultaneous Functional Magnetic Resonance and Two Dimensional Optical Imaging Spectroscopy -- Neurovascular Coupling Investigated by Simultaneous Optical Coherence Tomography and Electrophysiology -- Studying Adenosine and Cerebral Blood Flow (CBF) Using Pharmacological Methods -- Imaging Vasodynamics in the Awake Mouse Brain with Two-Photon Microscopy -- Neurovascular Coupling in the Deep Brain Using Confocal Fiber-Optic Endomicroscopy -- Non-Invasive Neural Imaging and Tissue Oxygenation in the Visual System -- High-Resolution Wide-Field Optical Imaging of Micro-Vascular Characteristics: From the Neocortex to the Eye -- Optical Intrinsic Signal Imaging for Elucidating Functional Structures in Higher Visual Area -- Closed Cranial Window Applications in the Rat: Studies on Neurovascular Coupling Involving Pial Arterioles and the Glia Limitans -- Multi-Spectral Imaging of Blood Volume, Metabolism, Oximetry and Light Scattering -- Physiological Basis of BOLD fMRI Decreases -- Simultaneous Multi-Wavelength Optical Imaging of Neuronal and Hemodynamic Activity -- Non-Invasive Laser-Induced Photoacoustic Tomography for Functional Imaging -- Neurovascular Coupling in Relation to Cortical Spreading Depression -- Cerebral Blood Flow Imaging with Laser Speckle Contrast Imaging -- Cerebral Monitoring and Surveillance Using High Resolution Functional Optical Imaging
    Abstract: Neurovascular Coupling Methods brings the reader up to date with the current state-of-the-art techniques in measuring blood flow in the brain, with chapters describing different techniques, or combinations of techniques, applied to specific species in either healthy or abnormal brains. Opening with a section on techniques in normal somatosensory processing, the detailed volume continues with parts covering techniques in normal visual processing, epilepsy, and cerebrovascular diseases, such as ischemia, hemorrhage, and spreading depression.℗ As a collection in the popular Neuromethods series, this book contains the kind of thorough description and implementation advice that will lead to successful results in the lab. ℗ Authoritative and practical, Neurovascular Coupling Methods serves as an ideal guide for researchers aiming to further our knowledge of these coupling mechanisms in the hopes of ultimately understanding higher order functions such as language and memory and developing novel brain mapping techniques that can be employed in humans
    Pages: XIX, 332 p. 120 illus., 99 illus. in color. : online resource.
    ISBN: 9781493907243
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  • 2
    Keywords: Medicine ; Neurosciences ; Neurology ; Biomedicine ; Neurosciences ; Neurology ; Springer eBooks
    Description / Table of Contents: The Transcranial Magnetic Stimulation (TMS) Device and Foundational Techniques -- Transcranial Magnetic Stimulation (TMS) Safety Considerations and Recommendations -- Neuronavigation for Transcranial Magnetic Stimulation -- Reaching Deep Brain Structures: The H-Coils -- Single-Pulse Transcranial Magnetic Stimulation (TMS) Protocols and Outcome Measures -- Paired-Pulse Transcranial Magnetic Stimulation (TMS) Protocols -- Repetitive Transcranial Magnetic Stimulation (rTMS) Protocols -- Off-Line and On-Line “Virtual Lesion” Protocols -- State-Dependent Transcranial Magnetic Stimulation (TMS) Protocols -- Combination of Transcranial Magnetic Stimulation (TMS) with Functional Magnetic Resonance Imaging -- Electroencephalography During Transcranial Magnetic Stimulation: Current Modus Operandi -- Transcranial Magnetic Stimulation (TMS) Clinical Applications: Therapeutics -- Transcranial Magnetic Stimulation (TMS) Clinical Applications: Diagnostics -- A Review of Current Clinical Practice in the Treatment of Major Depression -- Protocol for Depression Treatment Utilizing H-Coil Deep Brain Stimulation -- Navigated Transcranial Magnetic Stimulation: Principles and Protocol for Mapping the Motor Cortex -- Speech Mapping with Transcranial Magnetic Stimulation
    Abstract: Transcranial Magnetic Stimulation seeks to enable new and existing practitioners to learn and follow established TMS protocols. The individual chapters in this detailed volume describe methods for single and multiple pulse TMS as well as methods for TMS coupled with associated techniques, from electromyography to neuroimaging. The collection promises to facilitate growth and exploration of clinical and experimental TMS. As a volume in the popular Neuromethods series, chapters include the kind of expert implementation advice that encourages successful and reproducible results. ℗ Authoritative and practical, Transcranial Magnetic Stimulation will serve not only as a good methodological introduction for those new to this growing field, but also as a source of continual reference for experienced practitioners when re-visiting older, exploring novel, or developing new concepts
    Pages: XI, 386 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493908790
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  • 3
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Cerebral Microdialysis: Research Technique or Clinical Tool? -- In Vivo Microdialysis to Study Striatal Dopaminergic Neurodegeneration -- Application of Reverse Microdialysis in Neuropharmacological Studies -- Microdialysis and Advances for Sampling Synaptic and Extrasynaptic Pools -- In Vivo Microdialysis for Measurement of Oxidative Metabolism in the Living Brain -- Quantitative In Vivo Microdialysis in Pharmacokinetic Studies -- Combination of In Vivo Microdialysis with Selective Electrochemical Detection for Online Continuously Monitoring Brain Chemistry -- Application of Spinal Microdialysis in Freely Moving Rats -- Monitoring Extracellular Monoamines with In Vivo Microdialysis in Awake Rats: A Practical Approach -- Intracerebral Human Microdialysis in Parkinson’s Disease -- Monitoring Extracellular Amino Acid Neurotransmitters and hROS by In Vivo Microdialysis in Rats: A Practical Approach -- Measurement of Neuropeptides in Dialysate by LC-MS -- Achieving High Temporal Resolution for In Vivo Measurements by Microdialysis -- Indirect Analysis of Nitric Oxide and Quantitation of Selective Nitric Oxide Synthase Inhibitors in Microdialysate Samples -- Determination of Histamine in Microdialysis Samples from the Rodent Brain by Column Liquid Chromatography -- Intracerebral Microdialysis in the Study of Limbic Seizure Mechanisms and Antiepileptic Drug Action Using Freely Moving Rats
    Abstract: Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fields from psychopharmacology, neurobiology, and physiology in animals and also humans. Microdialysis Techniques in Neuroscience focuses on the practical aspects of microdialysis in animal and human, highlighting current technical limitations and providing a vision of what is yet to come for the determination of the most disparate compounds in the brain. The booḱ€™s contents range from new techniques for detection and quantifying the release of several different neurotransmitters in vitro and in vivo, even in freely moving animals, to sophisticated use of reverse dialysis and the application of microdialysis in pharmacokinetic studies. Each of the sixteen chapters, in fitting with the spirit of the Neuromethods series, contain an introduction that gives a broad overview of a focused topic, followed by an extensive protocol on how the experiments are performed along with invaluable practical advice. ℗ Detailed and authoritative, Microdialysis Techniques in Neuroscience will be a valuable reference for students, neuroscientists, and physicians for the use of microdialysis in the study of brain functions and its clinical applications
    Pages: XVII, 341 p. 113 illus., 10 illus. in color. : digital.
    ISBN: 9781627031738
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  • 4
    Unknown
    Totowa, NJ : Humana Press
    Keywords: Medicine ; Neurosciences ; Psychiatry ; Laboratory animals ; Biomedicine ; Neurosciences ; Psychiatry ; Animal Models ; Springer eBooks
    Description / Table of Contents: Introduction: Binge Eating, Bulimia Nervosa, and Hedonic Overeating -- Binge-Prone vs. Binge-Resistant Rats and Their Concomitant Behavioral Profiles -- Binge Eating in Female Rats Induced by Yo-Yo Dieting and Stress -- Binge-Type Eating Induced by Limited Access to Optional Foods -- Assessment of Stress-Independent Binge-Like Eating Behavior in Mice -- Predicting and Classifying Rats Prone to Overeating Fat -- Modeling Binge Eating in Non-Human Primates -- Psychosocial Stress and Diet History Promote Emotional Feeding in Female Rhesus Monkeys -- Stressful Experiences in Early Life and Subsequent Food Intake -- Sham Feeding in Rats Translates Into Modified Sham Feeding in Women with Bulimia Nervosa and Purging -- Animal Models of Binge Eating Palatable Foods: Emergence of Addiction-Like Behaviors and Brain Changes in the Rat -- Deep Brain Stimulation for the Treatment of Binge Eating: Mechanisms and Preclinical Models -- Saccharin Preference in Rats: Relation to Impulsivity and Drug Abuse -- Food Seeking in Spite of Harmful Consequences -- Introduction: Anorexia and Undereating -- Food Restriction and Reward in Rats -- Activity-Based Anorexia in the Rat -- Food-Anticipatory Activity: Rat Models and Underlying Mechanisms -- Anorexia and Drugs of Abuse Abnormally Suppress Appetite, the Result of a Shared Molecular Signal Foul-Up -- The Anorectic Phenotype of the Anx/anx Mouse is Related to Hypothalamic Dysfunction -- Functional Magnetic Resonance Imaging in Awake Rats: Studies Relevant to Addiction and the Reward Circuitry
    Abstract: The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research.℗ Animal Models of Eating Disorders serves as a collection of detailed techniques contributed by experts in the field who are well-versed in the development and implementation of these models. Since eating disorders are complex and likely due to a combination of environmental, genetic, and social causes, the detailed chapters of this volume have been designed to highlight different contributing factors. Collectively, these chapters give a comprehensive and representative overview of both recently developed and classic methodologies used in the study of eating disorders.℗ Written for the popular Neuromethods series, this work contains the kind of thorough description and implementation advice that promises successful results. ℗ Authoritative and practical, Animal Models of Eating Disorders aims to aid researchers in the use of animal models to assist in their investigation and characterization of the behaviors and neurochemical alterations associated with these devastating disorders
    Pages: XVI, 379 p. 76 illus., 10 illus. in color. : digital.
    ISBN: 9781627031042
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  • 5
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton -- Visualizing In Vitro Trafficking -- Interaction of the Glycocalyx with the Actin Cytoskeleton -- Cytoskeleton Dynamics and Binding Factors -- Principles of Proteomic Approaches to the Cytoskeleton -- Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus -- Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes -- The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases -- Neurofilaments: Properties, Functions, and Regulation -- From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics -- Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton -- Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact -- Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis -- Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System -- Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
    Abstract: The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered.℗ The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system.℗ Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results. ℗ Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton
    Pages: XIV, 359 p. 52 illus., 31 illus. in color. : digital.
    ISBN: 9781627032667
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  • 6
    Keywords: Medicine ; Neurosciences ; Neurology ; Laboratory animals ; Biomedicine ; Neurosciences ; Neurology ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Spinal Cord: Functional Organization, Diseases, and Dysfunctions -- The Pathological Findings in Traumatic Injury to the Human Spinal Cord -- Acute Spinal Cord Injury In Vitro: Insight Into Basic Mechanisms -- Spinal Cord Injury: The Lamprey Model -- Dorsal Root Injury for the Study of Spinal Cord Injury Repair -- No-Laminectomy Spinal Cord-Transected Murine Model -- Spinal Cord Injury: The Rabbit Model -- The Cat Model of Spinal Cord Injury -- Using Naturally-Occurring Spinal Cord Injury in Domestic Dogs to Explore Novel Therapeutic Options -- Primate Models of Spinal Repair -- Animal Models of Spinal Cord Ischemia -- Magnetic Resonance Imaging of Experimental Spinal Cord Injury -- Spinal Cord Injuries: Principles and Methods for Outcome Assessment -- Spinal Cord Injury: Modern Clinical Management and Its Correlation to Advances in Basic Science
    Abstract: The development of treatment strategies that can help patients with spinal cord injury to regain lost functions and an improved quality of life is a major medical challenge, and experimental spinal cord research has to meet these challenges by resolving fundamental problems, establishing a basis for possible novel treatment strategies of spinal cord injury, and motivating their clinical translation.℗ In Animal Models of Spinal Cord Repair, expert researchers examine a broad range of experimental models for research on spinal cord injury, how they have contributed to our current state of knowledge, and what their advantages are in the further advancement of spinal cord repair.℗ With models from simple lamprey to non-human primates, the information presented is intended to guide the implementation of animal models for spinal cord repair as well as to raise the awareness of the relevance of experimental models which may not be in the current mainstream of this research.℗ As a part of the Neuromethods series, this work contains the kind of detailed description and implementation advice to guarantee successful results in the laboratory. ℗ Comprehensive and cutting-edge, ℗ Animal Models of Spinal Cord Repair presents the background information and hands-on methods descriptions, as well as the basic and clinical issues, needed to stimulate and guide researchers with different backgrounds towards the development of improved strategies for functionally relevant repair of the injured human spinal cord
    Pages: XII, 336 p. 49 illus., 36 illus. in color. : digital.
    ISBN: 9781627031974
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  • 7
    Keywords: Medicine ; Human Physiology ; Neurosciences ; Biomedicine ; Neurosciences ; Human Physiology ; Springer eBooks
    Description / Table of Contents: Optogenetic Stimulation of Neurons in the Brainstem of Freely Behaving Rats and Mice -- Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex responses in the In Situ Arterially-Perfused Preparation -- Excitatory Responses to Microinjection of Glutamate Depend on Dose not Volume: A Meta-Analysis of Studies in Rat RVLM -- Acute Activation and Inhibition of the Sympathetic Baroreceptor Reflex -- Pharmacological Dissection of G Protein-Mediated Second Messenger Cascades in Neurons -- In Vivo Manipulation of Intracellular Signalling Pathways -- Identification of Spinally Projecting Neurons in the Rostral Ventrolateral Medulla In Vivo -- Microionotophoretic Study of Individual Neurons During Intracellular Recording -- Neurobiotin Electroporation for Combined Structural and Functional Analysis of Neurons in Developing Mouse Brain Slices -- Juxtacellular Neuronal Labelling, Physiological Characterization, and Phenotypic Identification of Single Neurons In Vivo -- Analysis of Sympathetic Nerve Activity Analysis of Sympathetic Nerve Activity -- Insight into Autonomic Nervous System Control of Heart Rate in the Rat Using Analysis of Heart Rate Variability and Baroreflex Sensitivity -- Neurophysiological Recording of the Compound Muscle Action Potential for Motor Unit Number Estimation in Mice -- Animal Models of Neuropathic Pain Due to Nerve Injury -- Detection of Sensitized Nerve Responses: Dorsal Root Reflexes, Live Cell Calcium, and ROS Imaging -- Visual Evoked Potential Recording in Rodents -- The Visual Evoked Potential in Humans -- Improving Motor Activation Patterns after Stroke with Wii-Based Movement Therapy
    Abstract: Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools.℗ In Stimulation and Inhibition of Neurons, experts in the field provide an overview of modern methods for generating lesions as well as for stimulating and inhibiting neural pathways.℗ Many new techniques such as optogenetics and the use of the in situ perfused preparation are examined, while, in other sections, the use and validity of more well-known approaches are reassessed. Written for the Neuromethods series, chapters examine their respective topics thoroughly and include the kind of detail and implementation advice that ensures successful results in the laboratory. ℗ Authoritative and cutting-edge, Stimulation and Inhibition of Neurons serves as an ideal guide for researchers seeking to gain further knowledge of the complex functions of the brain
    Pages: XII, 319 p. 73 illus., 15 illus. in color. : digital.
    ISBN: 9781627032339
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  • 8
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Identification of Glutamatergic Neurons -- Single Nanoparticle Tracking: A Method for Investigating the Surface Dynamics of Glutamate Receptors -- Positron Emission Tomography of Metabotropic Glutamate Receptors -- NMR Spectroscopy of Brain Glutamate Function -- Imaging Glutamate with Genetically Encoded Fluorescent Sensors -- Measurement of Astrocytic Glutamate Release using Genetically-Encoded Probe Combined with TIRF Microscopy -- Drugs to Alter Extracellular Concentration of Glutamate: Modulators of Glutamate Uptake Systems -- Drugs to Tune Up Glutamatergic Systems: Modulators of Glutamate Metabotropic Receptors -- Glutamatergic Synthesis, Recycling, and Receptor Pharmacology at Drosophila and Crustacean Neuromuscular Junctions -- Optical Control of Glutamate Receptors of the NMDA-Kind in Mammalian Neurons, with the use of Photoswitchable Ligands -- In Vivo Electrochemical Studies of Optogenetic-Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays -- Separation-Based Sensors using Microchip Electrophoresis with Microdialysis for Monitoring Glutamate and Other Bioactive Amines -- Brain Glutamate Monitoring by Microdialysis and Separation Methods with a Special Focus on Capillary Electrophoresis with Laser-Induced Fluorescence Detection -- In Vivo Determination of Glutamate Uptake by Brain Microdialysis -- Enzymes of Glutamate System -- The Glutamatergic System as Potential Clinical Biomarkers for Blood and Cerebrospinal Fluid Monitoring -- Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord
    Abstract: This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry
    Pages: XXII, 565 p. 136 illus., 101 illus. in color. : online resource.
    ISBN: 9781493972289
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  • 9
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Brain Derived Neurotrophic Factor and the €œÁttivit© plastic dei neuron퀝, the Neuronal Plasticity as Defined by Ernesto Lugaro (1870-1940) -- Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes -- Detecting Single and Multiple BDNF Transcripts In Situ in Neuronal Cultures and Brain Sections -- Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons -- Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from rare of Limited Samples of Adult and Aged Central Neurons -- Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence -- Methodology for Detecting and Tracking brain-Derived Neurotrophic Factor Complexes in Neurons using Single Quantum Dots -- Recording Activity-Dependent Release of BDNF from Hippocampal Neurons -- Ultrastructural Localization of BDNF and trkB Receptors -- Analysis of TrkB Receptor Activity using FRET Sensors -- BDNF-Induced Intracellular Signaling -- A Microfluidic Culture Platform for Neurotrophin Signaling Studies -- BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-azidohomoalanine -- Proteomic Tools to Study the Effect of BDNF on de novo Protein Synthesis -- BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus in Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation
    Abstract: This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF). The book provides the tools essential to any researcher that intends to explore the peculiar regulation of this neurotrophin from gene expression to its release and the signaling by TrkB receptors, as well as to study the neuronal responses to BDNF or the role of neurotrophin in various neurological diseases. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting edge and comprehensive, Brain-Derived Neurotrophic Factor(BDNF) is a valuable resource for researchers who want to further study the diversity of physiological roles of this important growth factor
    Pages: XII, 258 p. 35 illus., 31 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493989706
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  • 10
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Proteomics ; Neurosciences ; Proteomics ; Springer eBooks
    Description / Table of Contents: Neuroproteomics: The Methods -- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice -- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue -- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics -- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3) -- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip -- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes -- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry -- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons -- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures -- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data -- 2D-DIGE as a Tool in Neuroproteomics -- Neuropeptidomics of the Mammalian Brain -- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides -- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder
    Abstract: This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms
    Pages: XIII, 218 p. 55 illus., 37 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493996629
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