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  • 11
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH,℗ and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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  • 12
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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  • 13
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: The℗ Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves and their use in Neurobiological Research -- A Reliable Reversible Bonding Method for Perfused Microfluidic Devices -- Bridging Two Cultures: Minimalistic Networks Prepared by Microfluidic Arraying and Open Access Compartments for Electrophysiology -- Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfluidic Chip -- Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level -- Campenot Cultures and Microfluidics Provide Complementary Platforms for Spatial Study of Dorsal Root Ganglia Neurons -- Development of Microfluidic Devices for the Manipulation of Neuronal Synapses -- Use of a 3 Compartment Microfluidic Device to Study Activity Dependent Synaptic Competition -- Multi-Compartment Neuron-Glia Co-Culture Microsystem -- Compartmentalized Microfluidic Platforms as Tool of Choice to Study the Interaction Between Neurons and Osteoblasts -- A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit -- Compartmentalized Microfluidics for In Vitro Alzheimeŕ€™s Disease Studies -- Selective Biochemical Manipulation of Twin Neuronal Networks on Micro Electrode -- Compartmentalized Synapse Microarray for High-Throughput Screening
    Abstract: This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition, and compartmentalized devices for pharmacological research and drug discovery. Written in the popular℗ Neuromethods℗ series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. ℗ Authoritative and practical,℗ Microfluidic and Compartmentalized Platforms for Neurobiological Research℗ provides practical skills needed to fabricate, use microfluidics, and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience
    Pages: XIII, 248 p. 72 illus., 63 illus. in color. : online resource.
    ISBN: 9781493925100
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  • 14
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: In utero Electroporation of the Mouse Embryo -- Exo utero Electroporation of the Mouse Embryo -- In Vivo Electroporation of the Postnatal Rodent Forebrain -- Electroporation in the Rodent Retina in vivo and in vitro -- Electroporation in the Developing Mouse Olfactory Bulb -- Cell Migration Analysis of Cortical Interneurons after Electroporation -- Characterization of Newborn Interneurons In The Mouse Olfactory Bulb Using Postnatal Electroporation -- Genetic Manipulation Of Gyrencephalic Carnivores using in utero electroporation -- Electroporation for the Chick Embryonic CNS -- Targeting the Zebrafish Nervous System using In Vivo Electroporation -- Gene Transfer into Cultured Mammalian Embryos by Electroporation -- Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures -- Electroporation of Dissociated Hippocampal Neurons -- Tol2-Mediated Transgenesis And Its Application To Electroporation -- Control of Gene Expression In Neurons by the use of in vivo Electroporation and the Tetracycline System
    Abstract: This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish.℗ Recent advances of experiments using the tetracycline-regulated gene expression and Tol2 transposon systems are also included. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. ℗ Practical and authoritative, Electroporation Methods in Neuroscience serves to aid scientists in the further study into this crucially important way to study cells
    Pages: XII, 199 p. 71 illus., 58 illus. in color. : online resource.
    ISBN: 9781493924592
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  • 15
    Unknown
    Totowa, NJ : Humana Press
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating Laser Excitation -- Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes -- Carbon-Fibre Amperometry in the Study of Exocytosis -- Imaging of Insulin Exocytosis from Pancreatic Beta Cells -- Functional, Quantitative, and Super-Resolution Imaging and Spectroscopic Approaches for Studying Exocytosis -- Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells -- Measuring Exocytosis in Endocrine Tissue Slices -- Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents -- Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomes -- Isolated Neurohypophysial Terminals: Model for Depolarization-Secretion Coupling -- The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca2+-Triggered Steps of Regulated Exocytosis
    Abstract: Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.℗ The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.℗ The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.℗ Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results. ℗ Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field
    Pages: XIII, 248 p. 70 illus., 36 illus. in color. : online resource.
    ISBN: 9781627036764
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  • 16
    Keywords: Neurosciences ; Neurobiology ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Semiconductor Quantum Dots for Visualization and Sensing in Neuronal Cell Systems -- Injection of Adeno-Associated Virus Containing Optogenetic and Chemogenetic Probes into the Neonatal Mouse Brain -- Guide to Transcranial Imaging of Sound-Evoked Activity in the Auditory Cortex of GCaMP6s Mice In Vivo -- A Novel Pre-Clinical Rat Model of Alzheimer’s Disease -- Using Zebrafish to Study and Visualize Barriers of the CNS -- Photometric Patch Electrode to Simultaneously Measure Neural Electrical Activity and Optical Signal in the Brain Tissue -- Patch-Clamp Fluorometry and Its Applications to the Study of Ion Channels -- Modeling Early-Onset Post-Ischemic Seizures in Adult and Aging Mice -- Modeling Psychological Trauma in Rats -- Extending the Life-Span of Acute Neuronal Tissue for Imaging and Electrophysiological Studies -- Confocal Imaging of Fast Flash Photolysis of Caged Compounds in Cultured Neurons -- Analysis of the Functional States of an Astrocyte Syncytium -- Assessment of Behavioral Dysfunction following Experimental Traumatic Brain Injury (TBI)
    Abstract: This volume explores the latest techniques and improved methods used to study important neurobiological and associated neuropathological conditions. Chapters cover topics such as the application of semiconductor quantum dots as photostable fluorophores used for labeling and tracking; GCaMP6 transcranial imaging to monitor neural activity; the patch-clamping technique allowing simultaneous monitoring of membrane currents and optical signals; and quantifying behavioral dysfunction caused by traumatic brain injury (TBI). In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Basic Neurobiology Techniques is a valuable resource for novel and expert researchers interested in expanding their knowledge of this developing field
    Pages: XII, 352 p. 129 illus., 106 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999446
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  • 17
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example -- Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Receptor-Receptor Interactions using Radioligand Binding Autoradiography -- On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction -- Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region -- Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches -- Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats -- In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor-Receptor Interactions to the Central Nervous System Signaling -- Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety -- Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior -- Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution -- Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays -- Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System -- Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells -- Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET -- Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches -- Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization -- Conformational Profiling of the 5-HT2A Receptor Using F1AsH-BRET -- Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor-Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions -- Detection, Analysis, and Quantification of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay -- Unraveling the Functions of Endogenous Receptor Oligomers in the Brain using Interfering-Peptide: The Example of D1R/NMDAR Heteromers -- Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers
    Abstract: This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study
    Pages: XIX, 346 p. 63 illus., 46 illus. in color. : online resource.
    ISBN: 9781493985760
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  • 18
    Unknown
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Introduction -- Stroke -- Disorders of Consciousness -- Traumatic Brain Injury -- Parkinson’s Diseae -- Dystonia -- Meningitis -- Multiple Sclerosis -- Alzheimer’s Disease -- Tumors of the Central Nervous System -- Spinal Cord Injury -- Peripheral Neuropathy -- Epilepsy -- Future of Clinical Trials in Neurology
    Abstract: This volume provides in-depth descriptions and discussion of clinical trial designs based on novel data analysis of high-quality trials for 11 different neurological conditions in order to ultimately improve the quality of care in neurology. Some of the conditions discussed in this book are stroke, traumatic brain injury, Alzheimer’s disease, epilepsy, and tumors of the central nervous system. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your research center and clinical investigation. Thorough and comprehensive, Clinical Trials in Neurology is a valuable guide for individuals in the research, education, and clinical practice fields, specifically in the fields of medicine, physical therapy, occupational therapy, nursing, and public health. This volume is also an important resource for clinicians aiming to improve their understanding in methodology of clinical trials
    Pages: XII, 616 p. 109 illus., 95 illus. in color. : online resource.
    ISBN: 9781493978809
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  • 19
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: An Overview of the Blood-Brain Barrier -- Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics -- Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modelling of the Blood-Brain Barrier -- In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems -- In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress in Brain Microvascular Endothelial Cell Phenotype -- Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier -- Cell Penetrating Peptides as Theranostics against Impaired Blood-Brain barrier Permeability: Implications for Pathogenesis and Therapeutics Treatment of Neurodegenerative Disease -- Microbial Translocation of the Blood-Brain Barrier -- Transport across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System -- Drosophila as a Model to Study the Blood-Brain Barrier -- Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier -- Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents -- Experimental Tools to Study the Regulation and Function of the Choroid Plexus -- Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid acorss the Blood-Cerebrospinal Fluid Barrier -- In Vivo Analysis to Study Transport across the Blood-Retinal Barrier -- Increasing BBB Permeability via Focused Ultrasound: Current Methods in Pre-Clinical Research -- Evaluation of Blood-Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evan’s Blue Extravasation -- Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy -- Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECT -- Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage Measurement
    Abstract: This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches
    Pages: XIII, 367 p. 77 illus., 54 illus. in color. : online resource.
    ISBN: 9781493989461
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  • 20
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Three-Dimensional Cultures of Human Neural Stem Cells: An Application for Modeling Alzheimer’s Disease Pathogenesis -- Neural Stem Cell Fate Control on Micropatterned Substrates -- Dopaminergic and GABAergic Neuron In Vitro Differentiation from Embryonic Stem Cells -- Transfection of Cultured Primary Neurons -- Reprogramming of Mouse Fibroblasts to Induced Oligodendrocyte Progenitor Cells -- Isolation and Culture of Oligodendrocyte Precursor Cells from Pre- and Postnatal Rodent Brain -- Schwann Cell Isolation and Culture Reveals the Plasticity of these Glia -- Generation of Cerebral Organoids Derived from Human Pluripotent Stem Cells -- Development of Mouse Cell Based In Vitro Blood-Brain Barrier Models -- Microfluidic Device for Studying Traumatic Brain Injury -- Multimodal Neural Stem Cell Research Protocols for Experimental Spinal Cord Injuries -- Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System -- Standardized Cryopreservation of Stem Cells -- Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies -- Essential Requirements for Setting Up a Stem Cell Laboratory
    Abstract: This volume provides comprehensive knowledge on the fundamental techniques and detailed laboratory protocols used in stem cell research and their potential application in the field of neuroscience. The chapters in this book cover detailed descriptions of techniques for isolation, maintenance, differentiation, and characterization of neural stem cells, and the development of in vitro stem cell-based neurological disease model. There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory. Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals. Cutting-edge and pragmatic, Stem Cell Technologies in Neuroscience is a valuable resource for academic and translational researchers, industry scientists, biology teachers, and life science graduate students to grasp the vital pulse of the rapidly growing field of stem cell research
    Pages: XIV, 240 p. 55 illus., 46 illus. in color. : online resource.
    ISBN: 9781493970247
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