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  • 1
    Keywords: Medicine ; Toxicology ; Enzymes ; Biomedicine ; Pharmacology/Toxicology ; Enzymology ; Springer eBooks
    Description / Table of Contents: Enzyme Kinetics in Drug Metabolism – Fundamentals and Applications -- Fundaments of Enzyme Kinetics -- Different Enzyme Kinetic Models -- Reversible Mechanisms of Enzyme Inhibitors and Resulting Clinical Significance -- Irreversible Enzyme Inhibition Kinetics and Drug-Drug Interactions -- Multi-Enzyme Kinetics and Sequential Metabolism -- Consideration of the Unbound Drug Concentration in Enzyme Kinetics -- Enzyme Kinetics of Oxidative Metabolism – Cytochromes P450 -- Enzyme Kinetics, Pharmacokinetics, Inhibition, and Regioselectivity of Aldehyde Oxidase -- Enzyme Kinetics of Conjugative Enzymes – PAPS -- Enzyme Kinetics of Uridine Diphosphate Glucuronosyltransferases (UGTs) -- Principles and Experimental Considerations for in vitro Transporter Interaction Assays -- Rationalising Under-Prediction of Drug Clearance from Enzyme and Transporter Kinetic Data – from in vitro Tools to Mechanistic Modeling -- The Structural Model for the Mass Action Kinetic Analysis of P-gp Mediated Transport Through Confluent Cell Monolayers -- Systems Biology Approaches to Enzyme Kinetics: Analyzing Network Models of Drug Metabolism -- Variability in Human in vitro Enzyme Kinetics -- Sources of Interindividual Variability -- Case Study 1. Practical Considerations with Experimental Design and Interpretation -- Case Study 2. Practical Analytical Considerations for Conducting in vitro Enzyme Kinetic Studies -- Case Study 3. Application of Basic Enzyme Kinetics to Metabolism Studies – Real Life Examples -- Case Study 4. Predicting the Drug Interaction Potential for Inhibition of CYP2C8 by Montelukast -- Case Study 5. Deconvoluting Hyperbilirubinemia: Differentiating Between Hepatotoxicity and Reversible Inhibition of UGT1A, MRP2 or OATP1B1 in Drug Development -- Case Study 6. Drug Transporters: in vitro Solutions for Translatable Outcomes -- Case Study 7. Compiled Aha Moments in Enzyme Kinetics: Authors’ Experiences
    Abstract: Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with ℗ enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. ℗ Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.℗ ℗
    Pages: XVIII, 538 p. 134 illus., 36 illus. in color. : online resource.
    ISBN: 9781627037587
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  • 2
    Keywords: Medicine ; Human Genetics ; Medical genetics ; Biomedicine ; Human Genetics ; Gene Function ; Springer eBooks
    Description / Table of Contents: RecTEPsy Mediated Recombineering in Pseudomonas syringae -- Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila -- Multiple Genetic Manipulations of DT40 Cell Line -- Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination -- Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells -- Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Fenomic DNA in Eukaryotic Cells by Small DNA Fragments -- Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction -- Triplex-Mediated Genome Targeting and Editing -- Targeting piggyBac Transposon Integrations in the Human Genome -- Gene Targeting in Human Induced Pluripotent Stem Cells with Adenoviral Vectors -- Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus -- Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1 -- Designing and Testing the Activities of TAL Effector Nucleases -- A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities -- Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correction -- CRISPR-Cas Mediated Targeted Genome Editing in Human Cells -- RNA-Guided Genome Editing of Mammalian Cells -- Nuclease-Mediated Double Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human Induced Pluripotent Stem Cells (hiPSCs) -- AAV-Mediated Gene Editing via Double-Strand Break Repair -- Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae -- A Southern Blot Protocol to Detect Chimeric Nuclease-Mediated Gene Repair -- High-Throughput Cellular Screening of Engineered Nuclease Activity Using the Single-Strand Annealing Assay and Luciferase Reporter -- An Unbiased Method for Detection of Genome-Wide Off Target Effects in Cell Lines Treated with Zinc Finger Nucleases -- Identification of Off-Target Cleavage Sites of Zinc Finger Nucleases and TAL Effector Nucleases Using Predictive Models -- Method for Retinal Gene Repair in Neonatal Mouse -- In utero Delivery of Oligodeoxynucleotides for Gene Correction -- Portal Vein Delivery of Viral Vectors for Gene Therapy for Hemophilia -- Gene Correction of Induced Pluripotent Stem Cells Derived from a Murine Model of X-Linked Chronic Granulomatous Disorder -- Efficient Transduction of Hematopoietic Stem Cells and its Potential for Gene Correction of Hematopoietic Diseases
    Abstract: Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy
    Pages: XVI, 463 p. 87 illus., 59 illus. in color. : online resource.
    ISBN: 9781627037617
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  • 3
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Laboratory animals ; Life sciences ; Cell Biology ; Developmental Biology ; Animal Models ; Springer eBooks
    Description / Table of Contents: Culturing Larvae of Marine Invertebrates -- Obtaining Gametes and Embryos of Ascidians -- Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis -- Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential -- Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses -- An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images -- TEM Analyses of Chaetognath Reproductive Organs -- SEM Analysis of Marine Invertebrate Gametes -- Imaging Neural Development in Embryonic and Larval Sea Urchins -- Preparation and Use of Sea Urchin Egg Homogenates -- Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata -- Isolating Specific Embryonic Cells of the Sea Urchin by FACS -- Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata -- Perturbations to the Hedgehog Pathway in Sea Urchin Embryos -- Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo -- Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte ℗ -- Maturation in Marine Nemertean Worms -- Multi-color Labeling in Developmental Gene Regulatory Network Analysis -- A 96́€“well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay -- Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines -- Tools for Sea Urchin Genomic Analysis -- Antibody Inhibition of Protein Activity in Starfish Oocytes -- Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish.℗ ℗
    Abstract: In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates
    Pages: XIV, 347 p. 69 illus., 14 illus. in color. : online resource.
    ISBN: 9781627039741
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  • 4
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: The Pre-mRNA Splicing Reaction -- Diversity and Evolution of Spliceosomal Systems -- Mechanisms of Spliceosomal Assembly -- Alternative Pre-mRNA Splicing -- Regulation of Alternative Pre-mRNA Splicing -- Introduction to Co-Transcriptional RNA Splicing -- Chromatin and Splicing -- Preparation of Splicing Competent Nuclear Extracts -- Preparation of Yeast Whole Cell Splicing Extract -- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase -- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract -- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract -- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation -- Isolation and Accumulation of Spliceosomal Assembly Intermediates -- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function -- Expression and Purification of Splicing Proteins from Mammalian Cells -- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis -- Cell-Based Splicing of Minigenes -- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA -- Antisense Methods to Modulate Pre-mRNA Splicing -- Using Yeast Genetics to Study Splicing Mechanisms -- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR -- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing -- Computational Approaches to Mine Publicly Available Databases -- Approaches to Link RNA Secondary Structures with Splicing Regulation -- Methods to Study Splicing from High-Throughput RNA Sequencing Data -- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq -- Predicting Alternative Splicing
    Abstract: Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come
    Pages: XI, 427 p. 66 illus., 34 illus. in color. : online resource.
    ISBN: 9781627039802
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  • 5
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Cerebral Angiogenesis During Development: Who’s Conducting the Orchestra? -- Cerebral Angiogenesis: A Realistic Therapy for Ischemic Disease? -- Vascular Normalization in Cerebral Angiogenesis: Friend or Foe? -- Pericytes and Adaptive Angioplasticity: The Role of Tumor Necrosis Factor Weak Inducer of Apoptosis (TWEAK) -- Analysis of Angiogenesis in the Developing Mouse Central Nervous System -- Hypoxia-Induced Angiogenesis and Capillary Density Determination -- The Middle Cerebral Artery Occlusion Model of Transient Focal Cerebral Ischemia -- A Mouse Model of Chronic Cerebral Hypoperfusion Characterizing Features of Vascular Cognitive Impairment -- A Mouse Model of Permanent Focal Ischemia: Distal Middle Cerebral Artery Occlusion -- A Method of Inducing Global Cerebral Ischemia -- Induction of Cerebral Arteriogenesis in Mice -- Vessel Painting Technique for Visualizing the Cerebral Vascular Architecture of the Mouse -- Examining Cerebral Angiogenesis in Response to Physical Exercise -- Histological Assessment of Angiogenesis in the Hypoxic Central Nervous System -- Examining Vascular Remodeling in the Hypoxic Central Nervous System -- Analysis of Cerebral Angiogenesis in Human Glioblastomas -- Quantitative Cerebral Blood Flow Measurements Using MRI -- Fluorescent Angiogenesis Models Using Gelfoam® Implanted in Transgenic Mice Expressing Fluorescent Proteins -- Laser Speckle Contrast Imaging to Measure Changes in Cerebral Blood Flow -- Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow -- Defining the Role Of HIF and Its Downstream Mediators in Hypoxic-Induced Cerebral Angiogenesis -- Inducible Gene Deletion in Glial Cells to Study Angiogenesis in the Central Nervous System -- Bone Marrow Chimera Experiments to Determine the Contribution of Hematopoietic Stem Cells to Cerebral Angiogenesis -- Novel Methods for Accurate Identification, Isolation, and Genomic Analysis of Symptomatic Micro-Environments in Atherosclerotic Arteries -- Induction of Brain Arteriovenous Malformation in the Adult Mouse -- Stimulation of Cerebral Angiogenesis by Gene Delivery -- Investigating the Role of Perlecan Domain V in Post-Ischemic Cerebral Angiogenesis -- Isolation and Culture of Primary Mouse Brain Endothelial Cells -- Purification of Endothelial Cells from the Rat Brain -- Generation of Primary Cultures of Bovine Brain Endothelial Cells and Setup of Co-Cultures with Rat Astrocytes -- Isolation and Culture of Primary Pericytes from Mouse Brain -- Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles -- A Neurovascular Blood-Brain Barrier In Vitro Model -- In Vitro Models of the Blood-Brain Barrier
    Abstract: Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process.℗ Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain
    Pages: XV, 442 p. 102 illus., 83 illus. in color. : online resource.
    ISBN: 9781493903207
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  • 6
    Unknown
    Totowa, N.J. : Humana Press
    Associated volumes
    Call number: QH506:60/65
    Keywords: Polymerase chain reaction / Laboratory manuals
    Pages: xi, 221 p. : ill.
    ISBN: 0896033449
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  • 7
    Keywords: Life sciences ; Human Genetics ; Bioinformatics ; Life sciences ; Bioinformatics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Concepts and Introduction to RNA Bioinformatics -- The Principles of RNA Structure Architecture -- The Determination of RNA Folding Nearest Neighbor Parameters -- Energy Directed RNA Structure Prediction -- Introduction to Stochastic Context Free Grammars -- An Introduction to RNA Databases -- Energy-based RNA Consensus Secondary Structure Prediction in Multiple Sequence Alignments -- SCFGs in RNA Secondary Structure Prediction: A Hands-on Approach -- Annotating Functional RNAs in Genomes using Infernal -- Class-specific Prediction of ncRNAs -- Abstract Shape Analysis of RNA -- Introduction to RNA Secondary Structure Comparison -- RNA Structural Alignments, Part I: Sankoff Based Approaches for Structural Alignments -- RNA Structural Alignments, Part II: Non-Sankoff Approaches for Structural Alignments -- De novo Discovery of Structured ncRNA Motifs in Genomic Sequences.,- Phylogeny and Evolution of RNA Structure -- The Art of Editing RNA Structural Alignments -- Automated Modeling of RNA 3D Structure -- Computational Prediction of RNA-RNA Interactions -- Computational Prediction of microRNA Genes -- MicroRNA Target Finding by Comparative Genomics -- Bioinformatics of siRNA Design -- RNA-protein Interactions: An Overview
    Abstract: The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes.℗ However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere.℗ However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms.℗ Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. ℗ Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics
    Pages: XI, 533 p. 120 illus., 46 illus. in color. : online resource.
    ISBN: 9781627037099
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  • 8
    Keywords: Chemistry ; Biotechnology ; Regenerative Medicine ; Tissue Engineering ; Chemistry ; Biotechnology ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: Cartilage Tissue Engineering: What Have We Learned in Practice? -- Human Foetal and Adult Chondrocytes -- Mesenchymal Stem Cells Derived from Human Bone Marrow -- Mesenchymal Stem Cells Derived from Human Adipose Tissue -- Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors -- Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes -- Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis -- Hydrogels with Tunable Properties -- Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration -- Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft -- Bioprinted Scaffolds for Cartilage Tissue Engineering -- Scaffolds for Controlled Release of Cartilage Growth Factors -- Nanostructured Capsules for Cartilage Tissue Engineering -- Stratified Scaffolds for Osteochondral Tissue Engineering -- Mechanobioreactors for Cartilage Tissue Engineering -- Shear and Compression Bioreactor for Cartilage Synthesis -- Microbioreactors for Cartilage Tissue Engineering -- Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and Autograft): Construct Validation -- Proteomic Analysis of Engineered Cartilage -- Mechanical Testing of Cartilage Constructs
    Abstract: This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ́€˜smart́€™ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.℗
    Pages: XI, 290 p. 47 illus., 38 illus. in color. : online resource.
    ISBN: 9781493929382
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  • 9
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine -- Synthetic mRNA Reprogramming of Human Fibroblast Cells -- MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells -- Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors -- Reprogramming of Human Fibroblasts with Non-Integrating RNA Virus on Feeder-Free or Xeno-Free Conditions -- Inducing Pluripotency in Cattle -- Generation of Induced Pluripotent Stem Cells (iPSCs) From Adult Canine Fibroblasts -- Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a PiggyBac Transposon Delivery System and Temporal Control of Transgene Expression -- Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species -- Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cells -- Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation -- Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer -- Generation of Chimeras from Porcine Induced Pluripotent Stem Cells -- A Novel Method on Somatic Cell Nuclear Transfer with the Minimum Equipment -- Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer -- Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts -- Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells -- Genome Modification of Pluripotent Cells By Using Transcription Activator-Like Effector Nucleases (TALENs)
    Abstract: This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates;℗ alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming: Methods and Protocols is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis
    Pages: XI, 271 p. 27 illus., 20 illus. in color. : online resource.
    Edition: 1st ed. 2015.
    ISBN: 9781493928484
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  • 10
    Call number: QH506:60/482
    Keywords: Stem Cells ; Stem Cell Transplantation ; Regenerative Medicine / methods
    Pages: xix, 423 p. : ill.
    ISBN: 9781588297976
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