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  • 1
    Unknown
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.℗
    Pages: XII, 302 p. 66 illus., 63 illus. in color. : online resource.
    ISBN: 9781493974177
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  • 2
    Unknown
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume discusses the importance of imaging, fluid, and genetic biomarkers in detecting the earliest pre-symptomatic stages of Alzheimer’s disease (AD). The chapters in this book are separated into five parts. Part one explores the reasons why we need improved approaches for early detection and diagnosis of AD. Part two describes clinical and research techniques for preclinical AD diagnosis. Part three looks at the current methods in use to diagnose AD and how they can also be used for early detection in healthy older individuals. Parts four and five talk about technological advancements in AD diagnosis and ethical considerations in AD research. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Biomarkers for Preclinical Alzheimer’s Disease is a valuable resource for individuals interested in the use of biomarker strategies to improve early AD detection. This book will be of great interest to researchers and clinicians from both academia and industry
    Pages: XIII, 272 p. 39 illus., 33 illus. in color. : online resource.
    ISBN: 9781493976744
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  • 3
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Extracellular and intracellular recordings -- Theory of electrical transfer -- Amplifier -- Salt environment -- Micropipettes -- Spatiotemporal effects of synaptic input -- Perforated patch -- Isolation of synaptic current -- Fast and slow synaptic currents -- Measuring kinetics of synaptic current -- Measuring release probability -- Long-term measurements -- Measuring reversal potentials -- Amplitude -- Pre- and post-synaptic effect -- Run-up and run-down -- Kinetics of synaptic current -- Measurements of a single synapse -- Measurements of silent synapses -- Dendritic patch -- Electrophysiological tag -- In vivo electrophysiology
    Abstract: Electrophysiology is one of the finest approaches capable of detecting and analyzing electrical events in the brain. However, like all other experimental approaches, it has its limitations. This book was created to serve as a guide for those endeavoring to understand the function of electrophysiology and its underlying components: neurons, neural systems, and the brain. It introduces and highlights important topics in the field of electrophysiology, while also extending these topics to practical electrophysiological approaches through the perspective of the two authors. This book is ideal for graduate students or junior postdoctoral fellows interested in starting a journey in synaptic neuroscience
    Pages: XII, 263 p. 89 illus., 46 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493932740
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  • 4
    Keywords: Medicine ; Neurosciences ; Neurology ; Biomedicine ; Neurosciences ; Neurology ; Springer eBooks
    Description / Table of Contents: Operational Architectonics Methodology for EEG Analysis: Theory and Results -- Clinical Electroencephalography in the Diagnosis and Management of Epilepsy -- Effective Brain Connectivity from Intracranial EEG recordings: Identification of Epileptogenic Zone in Human Focal Epilepsies -- On the Effect of Volume Conduction on Graph Theoretic Measures of Brain Networks in Epilepsy -- Methods for Seizure Detection and Prediction: An Overview -- Graph-Theoretic Indices of Evaluating Brain Network Synchronization: Application in an Alcoholism Paradigm -- Time-Varying Effective Connectivity for Investigating the Neurophysiological Basis of Cognitive Processes -- Assessment of Sensory Gating Deficit in Schizophrenia Using a Wavelet Transform Methodology on Auditory Paired-Click Evoked Potentials -- Schizophrenia Assessment Using Single Trial Analysis of Brain Activity -- Phase Variants of the Common Spatial Patterns Method -- Estimation of Regional Activation Maps and Interdependencies from Minimum Norm Estimates of Magnetoencephalography (MEG) Data -- Blind Signal Separation Methods in Computational Neuroscience -- Current Trends in ERP Analysis Using EEG and EEG/ fMRI Synergistic Methods -- Computer-Based Assessment of Alzheimer’s Disease Employing fMRI and/or EEG: A Comprehensive Review
    Abstract: Modern Electroencephalographic Assessment Techniques: Theory and Applications presents numerous signal processing and connectivity analysis methodologies addressing a wide variety of clinical applications including epilepsy, schizophrenia, Alzheimer's disease, and even alcoholism. Among the different topics addressed, the neurophysiological basis of cognitive processes is also investigated. The goal is to provide a comprehensive overview of the most modern and widely established approaches mainly applied in, but not limited to, decomposing high resolution multichannel Electroencephalography (EEG) and Magnetoencephalography (MEG) signals into functional interconnected brain regions. Synergistic approaches linking both EEG/ MEG and functional Magnetic Resonance Imaging (fMRI) techniques are also discussed. In line with the popular Neuromethods series, chapters present the theoretical basis of each method along with prosperous application domains, in the form of a balanced mixture of theoretical tutorials, comprehensive reviews, and original research. Emphasis is given to the underlying assumptions, to technical matters that greatly affect the outcome of each proposed method, to the ambitions, and to the domain of application of each method. Furthermore, links to graph theory and visualization of connectivity motifs is also addressed in an attempt to better describe the functional characteristics of brain networks. ℗ Authoritative and practical, Modern Electroencephalographic Assessment Techniques: Theory and Applications touches upon both the biomedical and computational aspects of this exciting and rapidly evolving field, and will allow for a more in-depth, vital understanding of the braiń€™s complex underlying mechanisms
    Pages: XIX, 386 p. 72 illus., 67 illus. in color. : online resource.
    ISBN: 9781493912988
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  • 5
    Keywords: Medicine ; Human Genetics ; Neurosciences ; Neurology ; Biomedicine ; Neurosciences ; Human Genetics ; Neurology ; Springer eBooks
    Description / Table of Contents: Adeno-Associated Viral Vectors for Gene Delivery to the Nervous System -- Lentiviral Vectors for Gene Delivery to the Nervous System -- Gene Therapy for Parkinson’s Disease: AAV-5-Mediated Delivery of Glial Cell Line-Derived Neurotrophic Factor (GDNF) -- Gene Delivery and Gene Therapy for Alzheimer’s Disease -- Gene Therapy for Huntington’s Disease -- Gene Therapy Approaches to Promoting Axonal Regeneration After Spinal Cord Injury -- Gene Delivery to Neurons of the Dorsal Root Ganglia Using Adeno-Associated Viral Vectors -- Targeted Gene Therapy for Ischemic Stroke -- Adeno-Associated Viral Gene Therapy for Retinal Disorders -- Gene Therapy for Epilepsies -- AAV Gene Therapy Strategies for Lysosomal Storage Disorders with Central Nervous System Involvement -- Gene Therapy in Spinal Muscular Atrophy (SMA) Models Using Intracerebroventricular Injection into Neonatal Mice -- Gene Therapy for Chronic Pain: How to Manipulate and Unravel Pain Control Circuits from the Brain? -- Gene Therapy Approaches Using Reproducible and Fully Penetrant Lentivirus-Mediated Endogenous Glioma Models
    Abstract: This volume aims to explore the latest developments in adeno-associated viral and lentiviral vectors as well as the gene therapy strategies for℗ the most common neurological disorders, followed by chapters that include step-by-step guides to viral vector-based gene delivery in animal models used in the authorś€™ laboratories. Although safe gene manipulation in neural cells can be achieved, it may still be years away from efficacious gene-based treatment of neurological disorders such as Parkinson's and Alzheimeŕ€™s diseases due to the complexity of the underlying genetic/molecular mechanisms and the difficulty of developing reliable animal models. Gene Delivery and Therapy for Neurological Disorders seeks to aid researchers in this vital work.℗ Written in the popular Neuromethods series format, chapters include the kind of detailed description and expert implementation advice that leads to success in the lab. Meticulous and authoritative, Gene Delivery and Therapy for Neurological Disorders serves as an ideal guide for researchers attempting to explore the potentials of gene therapy for neurological disorders
    Pages: XIV, 359 p. 65 illus., 51 illus. in color. : online resource.
    ISBN: 9781493923069
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  • 6
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH,℗ and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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  • 7
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: The Evolution of Immunocytochemistry in the Dissection of Neural Complexity.-n Immunofluorescence℗ and Genetic Fluorescent Labeling℗ Techniques in the Drosophila℗ Nervous System -- Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris -- Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish℗ Brain -- Immunocytochemical℗ Phenotype℗ of Differentiating℗ Neurons.-Immunocytochemical Identification of Mammalian Differentiating Neurons℗ in the Process of Adult℗ Neurogenesis℗ in the Hippocampal Dentate Gyrus -- Identification of Proliferating and Migrating Cells by BrdU℗ and Other Thymidine Analogues. Benefits and Limitations -- Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System -- In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis℗ and Autophagy -- Immunocytochemical Detection of Intraneuronal AÎø℗ Peptides in Mouse Models of Alzheimer's Disease -- Purification℗ and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) -- Immunocytochemistry of Microglial Cells -- Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage -- Quantification of Immunocytochemical Colocalization in Neurons -- Immunogold℗ Electron Microscopic℗ Quantification℗ of Small Molecular℗ Compounds and Proteins℗ at Synapses and Other Neural Profiles -- Combined Immunocytochemistry℗ and Tracing℗ of Neural Connections -- Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing -- Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices -- Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse℗ Brain -- Array Tomography: A Novel High-Resolution Immunofluorescence Technique -- Single Molecule Localization Microscopy℗ to Study Neuronal℗ Microtubule Organization -- Studying Membrane Dynamics℗ of Proteins℗ Using Quantum Dots -- Optical℗ Protein℗ Patterning
    Abstract: This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.℗ Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research
    Pages: XIII, 473 p. 95 illus., 77 illus. in color. : online resource.
    ISBN: 9781493923137
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  • 8
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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  • 9
    Keywords: Medicine ; Neurosciences ; Laboratory animals ; Biomedicine ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Rodent Mazes and Memory: Continuing the Search for the Engram -- The Mazes -- The Watermaze -- Behavioral Consequences of Early Disruption and Injury to the Developing Brain: Studying Rodent Models -- Insights into Age-Related Cognitive Decline: Coupling Neurophysiological and Behavioral Approaches -- Organization and Structure of Brain Systems Supporting Memory -- Pharmacologically Manipulating Learning and Memory -- Stress and Spatial Maze Performance in the Rat -- Genetically Modified Rodent Models: A New Generation of Translational Cognitive Science -- Epigenetics of Cognition and Neurodegenerative Disorders -- The Importance of Incorporating Both Sexes and Embracing Hormonal Diversity When Conducting Rodent Behavioral Assays -- A Primer of Methods in Biobehavioral Research: Improving a Studý€™s Design, Analysis, and Write Up -- Optimal Guidelines for Monitoring and Maintaining Rodent Health for Behavioral Research: What́€™s Right with My Rodent? -- General Water-Escape Maze Testing Necessities and Guidelines for Rodents -- Water Radial-Arm Maze: 4 out of 8 Arms Platformed Protocol for Rodents -- Water Radial-Arm Maze: 7 Out of 8 Arms Platformed Protocol for Rodents -- Land Radial-Arm Maze: 8 Out of 8 Arms Baited with Food Protocol for Rodents -- Delayed Match to Sample Protocol for Rodents -- Morris Maze Protocol for Rodents -- The Visible Platform℗ Protocol for Rodents
    Abstract: This vibrant collection delivers a laboratory roadmap of testing cognition in the rodent. While rodents and mazes are the main center and focus of this book, many aspects in the field of learning and memory are discussed and detailed, spanning from the molecular to the human, with every chapter delivering a comprehensive review of historical milestones in order to provide context for past discoveries, new findings, and future studies. Didactic foundations, operational definitions, and theory, as well as practical experimental and apparatus set-up, data analysis, and interpretation instructions are included in the first part of the book, while part two contains step-by-step protocols, troubleshooting, and tips from experts in the field. Authoritative and inspirational, The Maze Book: Theories, Practice, and Protocols for Testing Rodent Cognition serves as a detailed and practical manual for scientists wishing to implement these tools in their laboratories and for scholars interested in this powerful field
    Pages: XVIII, 457 p. 75 illus., 46 illus. in color. : online resource.
    ISBN: 9781493921591
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  • 10
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example -- Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Receptor-Receptor Interactions using Radioligand Binding Autoradiography -- On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction -- Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region -- Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches -- Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats -- In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor-Receptor Interactions to the Central Nervous System Signaling -- Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety -- Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior -- Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution -- Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays -- Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System -- Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells -- Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET -- Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches -- Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization -- Conformational Profiling of the 5-HT2A Receptor Using F1AsH-BRET -- Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor-Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions -- Detection, Analysis, and Quantification of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay -- Unraveling the Functions of Endogenous Receptor Oligomers in the Brain using Interfering-Peptide: The Example of D1R/NMDAR Heteromers -- Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers
    Abstract: This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study
    Pages: XIX, 346 p. 63 illus., 46 illus. in color. : online resource.
    ISBN: 9781493985760
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