Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Preparation of single-stranded DNA for pyrosequencing by LATE-PCR -- A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly -- Improvement of LATE-PCR to prepare pyrosequencing template -- Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease -- Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA) -- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing -- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing -- A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit -- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample -- A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactions directly from double-stranded PCR products -- Improvement of pyrosequencing to allow multiplex SNP typing in a program -- Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase -- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing -- Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing -- Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system -- Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing -- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing -- Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing -- Construction of 3-plex barcodes for differential gene-expression analysis with pyrosequencing -- Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences -- Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes -- Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for CNV analysis -- Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides -- Pyrosequencer with a senor of a photo-diode array -- Pyrosequencing on acryl-modified glass chip -- Pyrosequencing on-chip based on a gel-based solid-phase amplification -- Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR -- Comparative gene expression analysis of breast-cancer related genes by multiplex pyrosequencing coupled with sequence-barcodes -- MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer.-℗ Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer -- Genotyping of pathogenic serotypes of S. suis with pyrosequencing -- Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing -- Detection of avian influenza A virus by pyrosequencing -- Genotyping of the alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material -- Pyrosequencing on a single cell
    Abstract: The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology. Divided into five parts, the book’s thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab. Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields
    Pages: XIV, 407 p. 156 illus., 44 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493933082
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    New York, NY : Springer
    Keywords: Medicine ; Infectious Diseases ; Medicine & Public Health ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Animal Coronaviruses: A Brief Introduction -- Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues -- Virus Neutralization Assay for Turkey Coronavirus Infection -- Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli -- Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen -- Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus -- Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses -- Real-Time RT-PCR Detection of Equine Coronavirus -- Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction -- Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus -- An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes -- A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus -- Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues -- PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene -- Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis -- Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction -- Molecular Characterization of Canine Coronavirus -- Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform -- Next Generation Sequencing for Porcine Coronaviruses
    Abstract: This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the℗ Springer Protocols Handbooks℗ series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.℗ Practical and authoritative,℗ Animal Coronaviruses℗ serves as an ideal reference for researchers examining a wide variety of coronavirus species in the℗ Coronaviridae
    Pages: X, 218 p. 25 illus., 15 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493934140
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction: Single cell biotechnology for uncultured microorganisms -- Single-cell cultivation in microfluidic devices -- Single-cell bacterial bioreporter assays to measure hydrocarbons -- Whole genome amplification of single bacterial cells -- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria -- Localisation of proteins within intact bacterial cells using fluorescent protein fusions -- Raman-FISH -- Single-cell metabolomics -- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division -- Single cell biotechnology for uncultured microorganisms -- Single cell and systems biology tools for biofuel production -- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT) -- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity -- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence
    Abstract: This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 204 p. 40 illus., 20 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783662491317
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: Life sciences ; Plant Ecology ; Botany ; Plant anatomy ; Zoology ; Anthropology ; Life sciences ; Plant Sciences ; Plant Ecology ; Anthropology ; Zoology ; Plant Anatomy/Development ; Springer eBooks
    Description / Table of Contents: Selection of Research Participants -- Methods and Techniques Used to Collect Ethnobiological Data -- Participatory Methods in Ethnobiological and Ethnoecological Research -- Oral History in Ethnobiology and Ethnoecology -- Procedures for Documentary Analysis in the Establishment of Ethnobiological Information -- Use of Visual Stimuli in Ethnobiological Research -- Methods in Research of Environmental Perception -- Methods for Data Collection in Medical Ethnobiology -- Introduction to Botanical Taxonomy -- Analysis of Vegetation in Ethnobotanical Studies -- Techniques for Collecting and Processing Plant Material and their Application in Ethnobotany Research -- An Introduction to Zoological Taxonomy and the Collection and Preparation of Zoological Specimens -- Sampling in Ethnobotanical Studies of Medicinal Plants -- Indigenous Populations - Some Peculiarities -- Participatory Mapping of the Terrestrial Landscape in Brazil: Experiences and Potentialities -- Chemical Tools Applied to Ethnobotanical Studies -- In vitro and in vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods -- Urban Ethnobotany: Theoretical and Methodological Contributions -- The Dynamics of Use of Non-traditional Ethnobiological Products: Some Aspects of Study -- Plant Anatomy in Ethnobotanical Research: Micrographic Techniques and Applications -- Methods and Techniques for Research on the Supply Chains of Biodiversity Products -- Methods and Techniques Applied to Ethnobotanical Studies of Timber Resources -- Ethnobiological Research in Public Markets -- Techniques for Analysis of Quantitative Ethnobiological Data: Use of Indices -- Ecological Methods in Ethnobotanical and Ethnobiological Research: Using Diversity Measurements and Richness Estimators -- Principles of Inferential Statistics Applied to Ethnobiology and Ethnoecology -- Problems and Perspectives in the Publication of Ethnobiological Studies -- “Returning” Ethnobiological Research to the Communities
    Abstract: Humans represent just one of many species that constitute the planet's biodiversity. Nevertheless, as the dominant species, humans have been the primary agent of the transformation of natural spaces. Therefore, the study of human interactions, biodiversity, and the environment that surrounds them is a basic tool for understanding the factors that bind human societies to natural resources. Within this context, ethnobiology is a promising discipline that can play a key role as a mediator of dialogue between different academic disciplines and traditional knowledge, a union essential in enabling contextualized and sustainable alternatives to exploitative practices and biodiversity management. ℗ Methods and Techniques in Ethnobiology and Ethnoecology introduces the basic techniques and methods traditionally used in ethnobiology and ethnoecology. Comprised of 28 chapters, the book covers the different qualitative and quantitative aspects of ethnobiology research methods, as well as methods from natural and social sciences that will be useful to both beginners and senior researchers. Written by internationally renowned experts in the fields, Methods and Techniques in Ethnobiology and Ethnoecology is a valuable resource for researchers and students interested in ethnobiology
    Pages: XV, 480 p. 53 illus., 18 illus. in color. : online resource.
    ISBN: 9781461486367
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: Plant Ecology ; Anthropology ; Plant anatomy ; Plant physiology ; Plant Systematics/Taxonomy/Biogeography ; Plant Ecology ; Anthropology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Preface -- Overview -- Preparation of qualitative research -- Execution of the qualitative research -- Participant observation and Field Journal -- Audio and video recording techniques for ethnobiological research -- Discourse of the collective subject as a method for analysis of data in ethnobiological research -- Qualitative data analysis -- The meta-analytical and macroecological approach of Ethnobiology -- Collection and analysis of risks associated with environmental changes -- Sensitivity to harvesting and local risk indexes in ethnobotanical research -- The use of multivariate tools in the study of traditional resource management systems -- Multidimensional analysis for testing conservation, ecological and ethnobiological hypotheses -- Ethnoecology in pluricultural contexts -- Ethnobotany and ethnoecology applied to historical ecology -- Biocultural collections -- Accessing the traditional knowledge on the use of cryptogams -- Ethical considerations and protocols in ethnoecological and ethnobiological research -- Challenges in ethnozoological research -- Methodology applied to geographical indication -- Recent advances in research methods and techniques -- Chemical profile and antioxidant activity of natural products.-Collection and extraction of fatty acids from animals and their possible applications in ethnopharmacological research -- Advanced methods in the chemical analysis of medicinal plants -- Population ecology of plant species submitted to extractivism -- Methods for measuring hunting pressure -- Rapid sampling techniques for vertebrate fauna -- Techniques for analyzing the sustainability of hunting -- Description of technological processes for optimization and enhancement of local uses -- Index
    Abstract: Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There have been, however, many advances in research as a result of interaction with different disciplines, but also due to more recent results, new original and interesting questions. This handbook provides the current state of the art methods and techniques in ethnobiology and ethnoecology, and related fields. This new volume, besides bringing new and original aspects of what is found in the literature, fills some of the gaps in volume one by including the most systematic and extensive treatment of methods and techniques in qualitative research. Along with the various methods covered in the individual chapters, the handbook also includes an extensive bibliography that details the current literature in the field
    Pages: XVI, 342 p. 42 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493989195
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: Life sciences ; Human Genetics ; Cell Biology ; Life sciences ; Cell Biology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part I Required Equipment and Probes for FISH Procedures -- Part II FISH Procedure -- Part III Material Suited for FISH Applications in Humans -- Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining -- Part V Interphase FISH -- Part VI Applications of FISH in Zoology, Botany and Microbiology -- Part VII Comparative Genomic Hybridization (CGH) and Array CGH
    Abstract: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences
    Pages: XIII, 606 p. : online resource.
    Edition: 2nd ed. 2017.
    ISBN: 9783662529591
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding -- Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes -- Lipid Bilayer Patterns Fabrication by One-Photon Lithography -- Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels -- Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy -- Fluorescence-Based Screening of Membrane Proteins for Structural Studies -- Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins -- Chemical Methods for Monitoring Protein Fatty-Acylation -- 〈Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research -- Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins -- Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis -- Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies
    Abstract: This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes
    Pages: XI, 270 p. 70 illus., 57 illus. in color. : online resource.
    ISBN: 9781493968367
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction to Microbial Quantitation, Community Profiling, and Array Approaches -- A cell extraction method for oily sediments -- Introduction to microplate MPN-enumeration of hydrocarbon degraders -- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs -- Microbial biomass and community analysis by PLFA analysis -- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities -- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities -- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis -- Microbial community profiling: SSCP and T-RFLP techniques -- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) – preparation, data handling and ecological analysis -- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment -- Studying protistan communities in hydrocarbon-contaminated environments
    Abstract: This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 252 p. 30 illus., 12 illus. in color. : online resource.
    Edition: 1st ed. 2017.
    ISBN: 9783662527788
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Keywords: Medicine ; Neurosciences ; Cell Culture ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Cell Culture ; Springer eBooks
    Pages: : digital
    Edition: 4th ed.
    ISBN: 9781607612926
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Electron microscopy protocols for the study of hydrocarbon-producing and -decomposing microbes: classical and advanced methods -- Protocol for laser scanning microscopy of microorganisms on hydrocarbons -- Fluorescence microscopy for microbiology -- Imaging bacterial cells and biofilms adhering to hydrophobic organic compounds-water interfaces -- Bacteria-mineral colloid interactions in biofilms - an ultrastructural and microanalytical approach -- Identification of microorganisms in hydrocarbon contaminated aquifer samples by fluorescence in-situ hybridization (CARD-FISH) -- Studies of the ecophysiology of single cells in microbial communities by (quantitative) microautoradiography and fluorescence in situ hybridization (MAR-FISH) -- Protocol for in situ detection of functional genes of microorganisms by Two-pass TSA-FISH -- Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy -- A Correlative Light-Electron Microscopy (CLEM) protocol for the identification of bacteria in animal tissue, exemplified by methanotrophic symbionts of deep-sea mussels
    Abstract: This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces. Three forms of fluorescence in situ hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are described for the ecophysiological analysis of functionally active microbes in samples. The methods presented will enable readers to obtain an ultrastructural picture of, and identify the key functional microbes in, samples under investigation. This in turn will constitute a key framework for the interpretation of information from other experimental approaches, such as physicochemical analyses and genomic investigations. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 174 p. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783662491348
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...