Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: Life sciences ; Biochemistry ; Plant Biochemistry ; Ecosystems ; Ecology ; Life sciences ; Plant Biochemistry ; Ecosystems ; Terrestial Ecology ; Animal Biochemistry ; Springer eBooks
    Description / Table of Contents: Physical and Chemical Properties of Nucleic Acids -- Sampling for Biostatistics -- Sampling of Microbiological Samples -- Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples -- Magnetic beads based nucleic acids purification for molecular biology applications -- Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications -- Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples -- High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems -- Portable Systems for Sample Lysis and Homogenization -- DNA and RNA extractions from mammalian samples -- Sample preparation for multiplex PCR assays for food and agriculture applications -- Nucleic acid sample preparation from stem cells -- Nucleic Acids Sample Preparation from Teeth/Dental Remains -- Nucleic Acids Sample Preparation for Food Traceability -- Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis -- Nucleic Acids Samples Preparation from Dairy Products and Milk -- Extraction of DNA from Plant Tissue: Review and Protocols -- DNA Extraction from Seeds -- RNA extractions from difficult to prepare and high starch content seeds -- Nucleic Acid and Protein Sample Preparation from Yeasts -- Nucleic acids purification from soil and environmental sources -- Extraction and Purification of Viral Nucleic Acids from Environmental Samples -- Preparation of nucleic acids from marine samples: applications to microbial ecology research -- Nucleic Acid Sample Preparation from Feces and Manure -- Bead-beating sample preparation for nucleic acids isolation from fecal samples -- Methods for extraction and purification of lignin and cellulose from plant tissues -- Separation and purification of glycans out of glycoproteins -- Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria
    Abstract: The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation
    Pages: XXIII, 406 p. 96 illus., 65 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493931859
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Designing bacteria to produce esters -- Protocols for the production and analysis of isoprenoids in bacteria and yeast -- Synthetic biology of hydrophobic polymer production -- Methods for recombinant rhamnolipid production -- Purification of peptide antimicrobials and thioether stabilized molecules produced in vivo by lantibiotic modification machineries -- Engineering transcription factor based biosensors for the detection of intracellular products -- Oxyfunctionalization of linear alkanes with a biosynthetic, self-sufficient, selective and soluble hydroxylase
    Abstract: This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 137 p. 40 illus., 20 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783662491270
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Preparing the hydrocarbon / crude oil -- High pressure cultivation of hydrocarbonoclastic aerobic bacteria -- In vitro high pressure incubation and activity measurement of deep-sea, methanogenic archaea -- Bioreactor design to emulate deep-sea hydrocarbon releases including formation of gas hydrates -- Mudflat benthic spill simulations -- Protocols for subtidal and deep sea benthic oil spill simulations -- Simulation of anoxic/oxic oscillations in crude oil-degrading bioreactors -- Protocols for microcosms for growing biofilms on hydrophobic substrates – a polyphasic approach to study biodiversity, metabolic activity and biofilm architecture -- Application of micro- and mesocosms experiments to pollutant effects in biofilms -- Mini sediment columns and two-dimensional sediment flow-through microcosms - versatile model systems for studying biodegradation of organic contaminants in groundwater ecosystems
    Abstract: This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. 〈 Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 173 p. 40 illus., 27 illus. in color. : online resource.
    Edition: 1st ed. 2017.
    ISBN: 9783662531082
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Protocols for the measurement of bacterial chemotaxis to hydrocarbons -- Determining the tendency of microorganisms to interact with hydrocarbon phases -- Protocols for measuring biosurfactant production in microbial cultures -- Protocol for the measurement of hydrocarbon transport in bacteria -- Bacterial solvent response and tolerance: cis-trans isomerization -- Analysis of PHB metabolism applying Tn5 mutagenesis in Ralstonia eutropha -- Phenotyping microarrays for the characterization of environmental microorganisms -- Control of the concentration of aquatic hydrocarbons by microorganisms -- Protocols for measuring methanogenesis -- Respiration rate determined by phosphorescence-based sensors -- Measuring the impact of hydrocarbons on rates of nitrogen fixation -- Protocols for measuring activity of sulphate-reducing bacteria in water injection systems -- Protocol for evaluating the biological stability of fuel formulations and their relationship to carbon steel biocorrosion -- Laboratory protocols for investigating microbial souring and potential treatments in crude oil reservoirs
    Abstract: This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes’ more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 244 p. 40 illus., 20 illus. in color. : online resource.
    Edition: 1st ed. 2017.
    ISBN: 9783662491409
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: Life sciences ; Plant Biochemistry ; Plant Ecology ; Plant anatomy ; Plant development ; Plant breeding ; Life sciences ; Plant Anatomy/Development ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Biochemistry ; Plant Ecology ; Springer eBooks
    Description / Table of Contents: Part I: Orchid Seeds in Plant Propagation and Conservation -- Understanding Seed and Protcorm Development in Orchids.-℗ Orchid Mycorrhizal Fungi:℗ Isolation and Identification Techniques -- Protocols for Establishing Fungi-Protocorm Culture -- Orchid Seed and Pollen: A Tool-kit for Long-term Storage, Viability Assessment and Conservation -- Part II: Orchid Micropropagation -- Orchid Seed Germination and Micropropagation I: Background information and Related Protocols -- Orchid Seed Germination and Micropropagation II: Media information and Composition -- Orchid Micropropagation: An Overview of Approaches and Methodologies -- Common Protocols in Orchid Micropropagation -- Micropropagation of Orchids by Using Bioreactor Technology -- Enhanced Growth and Development of Cymbidium and Phalaenopsis Plantlets Cultured in vitro Under Light-emitting Diodes -- Part III: Special Techniques -- Tracking Pollen Fates in Orchid Populations -- Cut-column Pollination Method to Overcome Pollination Barrier in Phalaenopsis -- In vitro Flowering and Breeding of Erycina pusilla -- Virus Elimination through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System -- Cryopreservation -- Haploid Seed Formation via Parthenogenesis in Bletilla -- A Protocol for the Induction of Polyploids in Phalaenopsis Orchids by in vitro Method without Using Anti-microtubule Agents -- Virus Detection -- Part IV: Cell and Molecular Biology and Transformation -- Genetic Transformation on Orchid species: An Overview of Approaches and Methodologies -- Recent Protocols on Genetic Transformation of Orchid Species -- The Use of Laser Microdissection to Investigate Cell Specific Gene Expression in Orchid Tissues -- Part V: Orchid Propagation and Maintenance -- Vegetative Propagation of Orchids -- Orchid Cultivation in Taiwan: Conventional and Innovative Methods -- Strategies in Orchid Health Maintenance -- Part VI: Showcasing Orchids -- Macro Flower Photography: An Introduction to Photographic Principles and Techniques -- An Introduction to Chinese Orchid Paintings -- Recent Advances in Orchid Seed Germination and Micropropagation
    Abstract: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.℗ This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing. This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.℗ The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an℗ in vitro℗ environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for on這s enjoyment
    Pages: XI, 524 p. 151 illus., 134 illus. in color. : online resource.
    ISBN: 9781493977710
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    facet.materialart.
    Singapore : Springer Singapore
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Abstract: This protocol book includes common and advanced research protocols for℗ Yersinia pestis℗ research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for researchers in this field, graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution etc
    Pages: VI, 290 p. 42 illus., 16 illus. in color. : online resource.
    ISBN: 9789811079474
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction to computer-assisted analysis in lipid and hydrocarbon microbiology -- Application of Ecological Network Theory -- Statistical tools for data analysis -- Statistical tools for study design and replication -- MG-RAST, a metagenomics service for analysis of microbial community structure and function -- Using QIIME to evaluate the microbial communities within hydrocarbon environments -- Biodegradation Prediction Tools -- Predicting protein interactions -- Syntax and Semantics of Coding in Python -- Protocols for calculating reaction kinetics and thermodynamics -- Modelling the environmental fate of hydrocarbons during bioremediation
    Abstract: This Volume covers protocols for in-silico approaches to hydrocarbon microbiology, including the selection and use of appropriate statistical tools for experimental design replication, data analysis, and computer-assisted approaches to data storage, management and utilisation. The application of algorithms to analyse the composition and function of microbial communities is presented, as are prediction tools for biodegradation and protein interactions. The basics of a major open-source programming language, Python, are explained. Protocols for calculating reaction kinetics and thermodynamics are presented, and modelling the environmental fate of hydrocarbons during bioremediation is explained. With the exception of molecular biology studies of molecular interactions, the use of statistics is absolutely essential for both experimental design and data analysis in microbiological research, and indeed in the biomedical sciences in general. Moreover, studies of highly varying systems call for the modelling and/or application of theoretical frameworks. Thus, while two protocols in this Volume are specific to hydrocarbon microbiology, the others are generic, and as such will be of use to researchers investigating a broad range of topics in microbiology and the biomedical sciences in general. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: XII, 180 p. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783662493106
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Keywords: Life sciences ; Human Genetics ; Cell Biology ; Life sciences ; Cell Biology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part I Required Equipment and Probes for FISH Procedures -- Part II FISH Procedure -- Part III Material Suited for FISH Applications in Humans -- Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining -- Part V Interphase FISH -- Part VI Applications of FISH in Zoology, Botany and Microbiology -- Part VII Comparative Genomic Hybridization (CGH) and Array CGH
    Abstract: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences
    Pages: XIII, 606 p. : online resource.
    Edition: 2nd ed. 2017.
    ISBN: 9783662529591
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding -- Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes -- Lipid Bilayer Patterns Fabrication by One-Photon Lithography -- Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels -- Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy -- Fluorescence-Based Screening of Membrane Proteins for Structural Studies -- Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins -- Chemical Methods for Monitoring Protein Fatty-Acylation -- 〈Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research -- Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins -- Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis -- Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies
    Abstract: This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes
    Pages: XI, 270 p. 70 illus., 57 illus. in color. : online resource.
    ISBN: 9781493968367
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding -- Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes -- Lipid Bilayer Patterns Fabrication by One-Photon Lithography -- Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels -- Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy -- Fluorescence-Based Screening of Membrane Proteins for Structural Studies -- Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins -- Chemical Methods for Monitoring Protein Fatty-Acylation -- 〈Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research -- Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins -- Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis -- Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies
    Abstract: This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes
    Pages: XI, 270 p. 70 illus., 57 illus. in color. : online resource.
    ISBN: 9781493968367
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...