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  • 1
    Keywords: Medicine ; Neurosciences ; Cell Culture ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Cell Culture ; Springer eBooks
    Pages: : digital
    Edition: 4th ed.
    ISBN: 9781607612926
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  • 2
    Keywords: Life sciences ; Plant Ecology ; Botany ; Plant anatomy ; Zoology ; Anthropology ; Life sciences ; Plant Sciences ; Plant Ecology ; Anthropology ; Zoology ; Plant Anatomy/Development ; Springer eBooks
    Description / Table of Contents: Selection of Research Participants -- Methods and Techniques Used to Collect Ethnobiological Data -- Participatory Methods in Ethnobiological and Ethnoecological Research -- Oral History in Ethnobiology and Ethnoecology -- Procedures for Documentary Analysis in the Establishment of Ethnobiological Information -- Use of Visual Stimuli in Ethnobiological Research -- Methods in Research of Environmental Perception -- Methods for Data Collection in Medical Ethnobiology -- Introduction to Botanical Taxonomy -- Analysis of Vegetation in Ethnobotanical Studies -- Techniques for Collecting and Processing Plant Material and their Application in Ethnobotany Research -- An Introduction to Zoological Taxonomy and the Collection and Preparation of Zoological Specimens -- Sampling in Ethnobotanical Studies of Medicinal Plants -- Indigenous Populations - Some Peculiarities -- Participatory Mapping of the Terrestrial Landscape in Brazil: Experiences and Potentialities -- Chemical Tools Applied to Ethnobotanical Studies -- In vitro and in vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods -- Urban Ethnobotany: Theoretical and Methodological Contributions -- The Dynamics of Use of Non-traditional Ethnobiological Products: Some Aspects of Study -- Plant Anatomy in Ethnobotanical Research: Micrographic Techniques and Applications -- Methods and Techniques for Research on the Supply Chains of Biodiversity Products -- Methods and Techniques Applied to Ethnobotanical Studies of Timber Resources -- Ethnobiological Research in Public Markets -- Techniques for Analysis of Quantitative Ethnobiological Data: Use of Indices -- Ecological Methods in Ethnobotanical and Ethnobiological Research: Using Diversity Measurements and Richness Estimators -- Principles of Inferential Statistics Applied to Ethnobiology and Ethnoecology -- Problems and Perspectives in the Publication of Ethnobiological Studies -- “Returning” Ethnobiological Research to the Communities
    Abstract: Humans represent just one of many species that constitute the planet's biodiversity. Nevertheless, as the dominant species, humans have been the primary agent of the transformation of natural spaces. Therefore, the study of human interactions, biodiversity, and the environment that surrounds them is a basic tool for understanding the factors that bind human societies to natural resources. Within this context, ethnobiology is a promising discipline that can play a key role as a mediator of dialogue between different academic disciplines and traditional knowledge, a union essential in enabling contextualized and sustainable alternatives to exploitative practices and biodiversity management. ℗ Methods and Techniques in Ethnobiology and Ethnoecology introduces the basic techniques and methods traditionally used in ethnobiology and ethnoecology. Comprised of 28 chapters, the book covers the different qualitative and quantitative aspects of ethnobiology research methods, as well as methods from natural and social sciences that will be useful to both beginners and senior researchers. Written by internationally renowned experts in the fields, Methods and Techniques in Ethnobiology and Ethnoecology is a valuable resource for researchers and students interested in ethnobiology
    Pages: XV, 480 p. 53 illus., 18 illus. in color. : online resource.
    ISBN: 9781461486367
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  • 3
    Keywords: Life sciences ; Plant Biochemistry ; Plant Ecology ; Plant anatomy ; Plant development ; Plant breeding ; Life sciences ; Plant Anatomy/Development ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Biochemistry ; Plant Ecology ; Springer eBooks
    Description / Table of Contents: Part I: Orchid Seeds in Plant Propagation and Conservation -- Understanding Seed and Protcorm Development in Orchids.-℗ Orchid Mycorrhizal Fungi:℗ Isolation and Identification Techniques -- Protocols for Establishing Fungi-Protocorm Culture -- Orchid Seed and Pollen: A Tool-kit for Long-term Storage, Viability Assessment and Conservation -- Part II: Orchid Micropropagation -- Orchid Seed Germination and Micropropagation I: Background information and Related Protocols -- Orchid Seed Germination and Micropropagation II: Media information and Composition -- Orchid Micropropagation: An Overview of Approaches and Methodologies -- Common Protocols in Orchid Micropropagation -- Micropropagation of Orchids by Using Bioreactor Technology -- Enhanced Growth and Development of Cymbidium and Phalaenopsis Plantlets Cultured in vitro Under Light-emitting Diodes -- Part III: Special Techniques -- Tracking Pollen Fates in Orchid Populations -- Cut-column Pollination Method to Overcome Pollination Barrier in Phalaenopsis -- In vitro Flowering and Breeding of Erycina pusilla -- Virus Elimination through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System -- Cryopreservation -- Haploid Seed Formation via Parthenogenesis in Bletilla -- A Protocol for the Induction of Polyploids in Phalaenopsis Orchids by in vitro Method without Using Anti-microtubule Agents -- Virus Detection -- Part IV: Cell and Molecular Biology and Transformation -- Genetic Transformation on Orchid species: An Overview of Approaches and Methodologies -- Recent Protocols on Genetic Transformation of Orchid Species -- The Use of Laser Microdissection to Investigate Cell Specific Gene Expression in Orchid Tissues -- Part V: Orchid Propagation and Maintenance -- Vegetative Propagation of Orchids -- Orchid Cultivation in Taiwan: Conventional and Innovative Methods -- Strategies in Orchid Health Maintenance -- Part VI: Showcasing Orchids -- Macro Flower Photography: An Introduction to Photographic Principles and Techniques -- An Introduction to Chinese Orchid Paintings -- Recent Advances in Orchid Seed Germination and Micropropagation
    Abstract: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.℗ This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing. This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.℗ The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an℗ in vitro℗ environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for on這s enjoyment
    Pages: XI, 524 p. 151 illus., 134 illus. in color. : online resource.
    ISBN: 9781493977710
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  • 4
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Abstract: This protocol book includes common and advanced research protocols for℗ Yersinia pestis℗ research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for researchers in this field, graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution etc
    Pages: VI, 290 p. 42 illus., 16 illus. in color. : online resource.
    ISBN: 9789811079474
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  • 5
    Keywords: Plant Ecology ; Anthropology ; Plant anatomy ; Plant physiology ; Plant Systematics/Taxonomy/Biogeography ; Plant Ecology ; Anthropology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Preface -- Overview -- Preparation of qualitative research -- Execution of the qualitative research -- Participant observation and Field Journal -- Audio and video recording techniques for ethnobiological research -- Discourse of the collective subject as a method for analysis of data in ethnobiological research -- Qualitative data analysis -- The meta-analytical and macroecological approach of Ethnobiology -- Collection and analysis of risks associated with environmental changes -- Sensitivity to harvesting and local risk indexes in ethnobotanical research -- The use of multivariate tools in the study of traditional resource management systems -- Multidimensional analysis for testing conservation, ecological and ethnobiological hypotheses -- Ethnoecology in pluricultural contexts -- Ethnobotany and ethnoecology applied to historical ecology -- Biocultural collections -- Accessing the traditional knowledge on the use of cryptogams -- Ethical considerations and protocols in ethnoecological and ethnobiological research -- Challenges in ethnozoological research -- Methodology applied to geographical indication -- Recent advances in research methods and techniques -- Chemical profile and antioxidant activity of natural products.-Collection and extraction of fatty acids from animals and their possible applications in ethnopharmacological research -- Advanced methods in the chemical analysis of medicinal plants -- Population ecology of plant species submitted to extractivism -- Methods for measuring hunting pressure -- Rapid sampling techniques for vertebrate fauna -- Techniques for analyzing the sustainability of hunting -- Description of technological processes for optimization and enhancement of local uses -- Index
    Abstract: Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There have been, however, many advances in research as a result of interaction with different disciplines, but also due to more recent results, new original and interesting questions. This handbook provides the current state of the art methods and techniques in ethnobiology and ethnoecology, and related fields. This new volume, besides bringing new and original aspects of what is found in the literature, fills some of the gaps in volume one by including the most systematic and extensive treatment of methods and techniques in qualitative research. Along with the various methods covered in the individual chapters, the handbook also includes an extensive bibliography that details the current literature in the field
    Pages: XVI, 342 p. 42 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493989195
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  • 6
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding -- Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes -- Lipid Bilayer Patterns Fabrication by One-Photon Lithography -- Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels -- Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy -- Fluorescence-Based Screening of Membrane Proteins for Structural Studies -- Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins -- Chemical Methods for Monitoring Protein Fatty-Acylation -- 〈Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research -- Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins -- Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis -- Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies
    Abstract: This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics. Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes
    Pages: XI, 270 p. 70 illus., 57 illus. in color. : online resource.
    ISBN: 9781493968367
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  • 7
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction to genetic, genomic and system analyses for communities -- Genomic analysis of pure cultures and communities -- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems -- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems -- Prokaryotic metatranscriptomics -- Defining a pipeline for metaproteomic analyses -- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing -- DNA and RNA-based stable isotope probing of hydrocarbon degraders -- Protein stable isotope probing (Protein-SIP) -- Protein extraction from contaminated soils and sediments -- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities -- Physiological modelling of complex bacterial communities -- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants
    Abstract: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 288 p. 50 illus., 30 illus. in color. : online resource.
    Edition: 1st ed. 2017.
    ISBN: 9783662504505
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  • 8
    Keywords: Life sciences ; Biochemistry ; Plant Biochemistry ; Ecosystems ; Ecology ; Life sciences ; Plant Biochemistry ; Ecosystems ; Terrestial Ecology ; Animal Biochemistry ; Springer eBooks
    Description / Table of Contents: Physical and Chemical Properties of Nucleic Acids -- Sampling for Biostatistics -- Sampling of Microbiological Samples -- Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples -- Magnetic beads based nucleic acids purification for molecular biology applications -- Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications -- Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples -- High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems -- Portable Systems for Sample Lysis and Homogenization -- DNA and RNA extractions from mammalian samples -- Sample preparation for multiplex PCR assays for food and agriculture applications -- Nucleic acid sample preparation from stem cells -- Nucleic Acids Sample Preparation from Teeth/Dental Remains -- Nucleic Acids Sample Preparation for Food Traceability -- Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis -- Nucleic Acids Samples Preparation from Dairy Products and Milk -- Extraction of DNA from Plant Tissue: Review and Protocols -- DNA Extraction from Seeds -- RNA extractions from difficult to prepare and high starch content seeds -- Nucleic Acid and Protein Sample Preparation from Yeasts -- Nucleic acids purification from soil and environmental sources -- Extraction and Purification of Viral Nucleic Acids from Environmental Samples -- Preparation of nucleic acids from marine samples: applications to microbial ecology research -- Nucleic Acid Sample Preparation from Feces and Manure -- Bead-beating sample preparation for nucleic acids isolation from fecal samples -- Methods for extraction and purification of lignin and cellulose from plant tissues -- Separation and purification of glycans out of glycoproteins -- Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria
    Abstract: The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation
    Pages: XXIII, 406 p. 96 illus., 65 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493931859
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  • 9
    Keywords: Life sciences ; Biochemical engineering ; Microbiology ; Life sciences ; Microbiology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers -- Protocols for the detection and chemical characterisation of microbial glycolipids -- Isolation of glycoprotein bioemulsifiers produced by marine bacteria -- Protocols for lipid extraction from wet algal biomass -- Isolation and characterization of lipid droplets from yeast -- Protocols for Isolation and Analysis of Polyhydroxyalkanoates -- Purification of lipid rafts from bacterial membranes -- Isolation and analysis of membrane phospholipids as biomarkers -- Protocols for purifying and characterizing integral membrane AlkB enzymes -- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242) -- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate -- Microcalorimetry as a general technique to characterize ligand binding
    Abstract: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes
    Pages: X, 188 p. 29 illus., 10 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783662491379
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  • 10
    Keywords: Life sciences ; Human Genetics ; Cell Biology ; Life sciences ; Cell Biology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part I Required Equipment and Probes for FISH Procedures -- Part II FISH Procedure -- Part III Material Suited for FISH Applications in Humans -- Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining -- Part V Interphase FISH -- Part VI Applications of FISH in Zoology, Botany and Microbiology -- Part VII Comparative Genomic Hybridization (CGH) and Array CGH
    Abstract: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences
    Pages: XIII, 606 p. : online resource.
    Edition: 2nd ed. 2017.
    ISBN: 9783662529591
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