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  • 1
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    New York. : Humana Press
    Associated volumes
    Call number: QH506:60/514(2)
    Keywords: T-Lymphocytes / immunology
    Pages: xi, 165 p : ill.
    Edition: 2nd ed.
    ISBN: 9781588295873
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  • 2
    Call number: QH506:60/473
    Keywords: Epidemiology ; Epidemiology / Methodology
    Pages: x, 321 p. : ill
    ISBN: 9781588299642
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  • 3
    Keywords: Life sciences ; Bioinformatics ; Biological models ; Biology / Data processing ; Life sciences ; Bioinformatics ; Systems Biology ; Computer Appl. in Life Sciences ; Springer eBooks
    Description / Table of Contents: Introduction to Biomedical literature text mining: Context and Objectives -- Accessing Biomedical Literature in the Current Information Landscape -- Mapping of Biomedical Text to Concepts of Lexicons, Terminologies and Ontologies -- Drug Interaction Text Mining -- Biological Information Extraction and Co-occurence Analysis: State of the Art and Perspectives -- Roles of Text Mining in Protein Function Prediction -- Functional Molecular Units for Guiding Biomarker Panel Design -- Mining Biological Networks from Full-text Articles -- Scientific Collaboration Networks using Biomedical Text -- Predicting future discoveries from current scientific literature -- Mining Emerging Biomedical Literature for Understanding Disease Associations in Drug Discovery -- Integrating Literature and Data Mining to Rank Disease Candidate Genes -- Role of Text Mining in Early Identification of Potential Drug Safety Issues -- Systematic Drug Repositioning using Text Mining -- Mining℗ the Electronic Health Record for Disease-Specific Knowledge
    Abstract: Biomedical Literature Mining,℗ discusses the multiple facets of modern biomedical literature mining and its many applications in genomics and systems biology. The volume is divided into three sections focusing on information retrieval, integrated text-mining approaches, and domain-specific mining methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Biomedical Literature Mining℗ is℗ designed as a useful bioinformatics resource in biomedical literature text mining for both those long experienced in, or entirely new to, the field
    Pages: XII, 288 p. 51 illus., 36 illus. in color. : online resource.
    ISBN: 9781493907090
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  • 4
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Embryology ; Life sciences ; Cell Biology ; Embryology ; Developmental Biology ; Springer eBooks
    Description / Table of Contents: General Aspects of Fertility and Infertility -- Genetics of Male Fertility -- Genetics of Female Infertility due to Anomalies of the Ovary and Mullerian Ducts -- Gene Polymorphisms in Female Reproduction -- Understanding the Spermatozoon -- Derivation of Human Embryonic Stem Cells (hESC) -- The Endocrinology of the Menstrual Cycle -- Assisted Reproductive Techniques -- Novel Markers of Male Infertility -- Luteal Phase Support in ART Treatments -- General Principles of Cryopreservation -- In Vitro Maturation of Immature Human Oocytes for Clinical Application -- GnRH Antagonist Based Protocols for In Vitro Fertilization -- Ovarian Stimulation for IVF: Mild Approaches -- IVF Stimulation: Protocols for Poor Responders -- Oocyte Retrieval and Quality Evaluation -- Sperm Retrieval and Quality Evaluation -- Treatment of Male Infertility -- Techniques for Slow Cryopreservation of Embryos -- Cryopreservation of Eggs -- Ovarian Tissue Cryopreservation and Transplantation. A Realistic, Effective Technology for Fertility Preservation -- Detection of Monogenic Disorders and Chromosome Aberrations by Preimplantation Genetic Diagnosis -- Embryo Culture and Selection: Morphological Criteria -- Embryo Selection using Metabolomics -- Embryo Transfer -- Safety of Assisted Reproductive Technologies -- Human Germ Cell Differentiation from Pluripotent Embryonic Stem Cells and Induced Pluripotent Stem Cells
    Abstract: Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell, and molecular biologists, and others in the biomedical sciences. The volume presents straight-forward manner best practice approaches for overcoming a host of fertility challenges.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ ℗ Authoritative and cutting-edge, Human Fertility: Methods and Protocols aids scientists in continuing to study assisted reproductive technologies
    Pages: XI, 584 p. 115 illus., 87 illus. in color. : online resource.
    ISBN: 9781493906598
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  • 5
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Efficient Library Preparation for Next-Generation Sequencing Analysis of Genome-Wide Epigenetic and Transcriptional Landscapes in Embryonic Stem Cells -- Analysis of Next-Generation Sequencing Data Using Galaxy -- edgeR for Differential RNA-seq℗ and ChIP-seq Analysis: An Application℗ to Stem℗ Cell Biology -- Use Model-based Analysis of ChIP-Seq (MACS) to Analyze Short Reads Generated by Sequencing Protein-DNA Interactions in Embryonic Stem Cells -- Spatial Clustering for Identification of ChIP-Enriched Regions (SICER) to Map Regions of Histone Methylation Patterns in Embryonic Stem Cells -- Identifying Stem Cell Gene Expression Patterns and Phenotypic Networks with AutoSOME -- Visualization and Clustering of High-Dimensional Transcriptome Data Using GATE -- Interpreting and Visualizing ChIP-seq Data with the seqMINER Software -- A Description of the Molecular Signatures Database (MSigDB) Website -- Use of Genome-Wide RNAi Screens to Identify Regulators of Embryonic Stem Cell Self-Renewal and Pluripotency -- Correlating Histone Modification Patterns with Gene Expression Data During Hematopoiesis -- In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes and Preimplantation Embryo Culture -- Derivation and Manipulation of Trophoblast Stem Cells from Mouse Blastocysts -- Conversion of Epiblast Stem Cells to Embryonic Stem Cells Using Growth Factors and Small Molecule Inhibitors -- Generation of Induced Pluripotent Stem Cells Using Chemical Inhibition and Three Transcription Factors -- Transdifferentiation of Mouse Fibroblasts and Hepatocytes to Functional Neurons -- Direct Lineage Conversion of Pancreatic Exocrine to Endocrine Beta-Cells In Vivo with Defined Factors -- Direct Reprogramming of Cardiac Fibroblasts to Cardiomyocytes Using MicroRNAs -- Reprogramming Somatic Cells into Pluripotent Stem Cells Using miRNAs
    Abstract: Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis.℗ Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation.℗ Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks
    Pages: X, 286 p. 150 illus., 142 illus. in color. : online resource.
    ISBN: 9781493905126
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  • 6
    Keywords: Medicine ; Medical laboratories ; Medical virology ; Emerging infectious diseases ; Medicine & Public Health ; Infectious Diseases ; Virology ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
    Abstract: Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research
    Pages: XIII, 403 p. 82 illus., 49 illus. in color. : online resource.
    ISBN: 9781493903481
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  • 7
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Computational Methods for Pseudogene Annotation Based on Sequence Homology.-℗ Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content -- Computational Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation -- Whole Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS -- Methods to Study the Occurrence and the Evolution of Pseudogenes Through a Phylogenetic Approach -- Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing,0 RNA Amplification for Pseudogene Detection Using RNA-Seq.-℗ GENCODE Pseudogenes -- Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features -- Proteomics Techniques for the Detection of Translated Pseudogenes -- Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation -- Pseudogenes: A Novel Source of Trans Acting Antisense RNAs -- Pseudogene-Derived Endogenous siRNAs and Their Function -- Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay -- Targeted and Robust Amplification of Mitochondrial DNA in the Presence of Nuclear Encoded Mitochondrial Pseudogenes Using ÎŒ29 DNA Polymerases -- Discrimination of Pseudogene and Parental Gene DNA Methylation Using Allelic Bisulphite Sequencing Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long Range PCR on Genomic DNA -- PMS2 Gene Mutational Analysis: RNA-Based Direct cDNA Sequencing to Circumvent Pseudogene Interference -- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era
    Abstract: Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality, but now known to be involved in complex regulatory circuits. After a few introductory chapters that overview the functions so far attributed to pseudogenes, this thorough volume delves into methods for pseudogene identification, for the detection of pseudogene transcription and translation, and for the study of the functions of pseudogenic RNA and proteins, as well as methods to avoid pseudogene detection when the focus of the research is their highly homologous parental counterparts.℗ As part of the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed descriptions and implementation advice that ensures successful results in the lab. ℗ Authoritative and practical, Pseudogenes: Functions and Protocols will contribute to the high interest of the scientific community toward pseudogenes, while stimulating the conception of pseudogene-centered research projects and providing experimental protocols that can facilitate their execution
    Pages: X, 319 p. 58 illus., 38 illus. in color. : online resource.
    ISBN: 9781493908356
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  • 8
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    New York, NY : Springer
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Description / Table of Contents: Basic Investigations in Saccharomyces cerevisiae -- Isolation of Yeast Nucleic Acids -- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe -- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry -- Yeast Two Hybrid Liquid Screening -- Targeted Mutagenesis of a Specific Gene in Yeast -- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae -- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy -- Assays for Autophagy II: Mitochondrial Autophagy -- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction -- Yeast Survival and Growth Assays -- Spontaneous Mutagenesis Assay -- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene -- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe -- Replicative and Chronological Life Span Assays -- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae -- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae -- Ligand Engineering Using Yeast Surface Display -- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
    Abstract: Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism
    Pages: X, 313 p. 57 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493907991
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  • 9
    Keywords: Life sciences ; Proteomics ; Biochemistry ; Life sciences ; Protein Science ; Proteomics ; Springer eBooks
    Description / Table of Contents: Survey of Shotgun Proteomics -- LC-MALDI-TOF/TOF for Shotgun Proteomics -- Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry -- GeLC-MS/MS Analysis of Complex Protein Mixtures -- IPG Strip-Based Peptide Fractionation for Shotgun Proteomics -- SILAC Yeast: From Labeling to Comprehensive Proteome Quantification -- Analysis of Proteome Dynamics in Mice by Isotopic Labeling -- Stable Isotope Labeling in Mammals (SILAM) -- Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale -- Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification -- Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL) -- Employing TMT Quantification in a Shotgun-MS Platform -- Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle -- Spectral Counting Label-Free Proteomics -- Quantification of Proteins by Label Free LC-MSE -- Bioinformatics for Proteomics: Opportunities at the Interface Between the Scientists, Their Experiments and the Community -- Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry -- Application of Shotgun Proteomics for Discovery-Driven Protein-Protein Interaction -- Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags -- Biomarker Verification Using Selected Reaction Monitoring and Shotgun Proteomics -- Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers -- The Secretome Analysis by High Throughput Proteomics and Multiple Reaction Monitoring (MRM) -- Preparation of Heteroelement-Incorporated and Stable Isotope-Labeled Protein Standards for Quantitative Proteomics -- One-Source Peptide/Phosphopeptide Ratio Standards for Accurate and Site-Specific Determination of the Degree of Phosphorylation -- Quantitative Glycoproteomics for N-Glycoproteome Profiling -- A Practical Recipe to Survey Phosphoproteomes -- Quantitation of the Phosphoproteome Using the Library Assisted eXtracted Ion Chromatogram (LAXIC) Strategy -- Fast, Efficient and Quality-Controlled Phosphopeptide Enrichment from Minute Sample Amounts Using Titanium Dioxide -- Quantifying Small Molecule-Induced Changes in Cellular Protein Expression and Post-Translational Modifications Using Isobaric Mass Tags -- Analysis of Protein Structure by Crosslinking Combined with Mass Spectrometry -- Top Down Proteomics by Means of Orbitrap Mass Spectrometry
    Abstract: Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.℗ Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species
    Pages: XIV, 492 p. 81 illus., 48 illus. in color. : online resource.
    ISBN: 9781493906857
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  • 10
    Keywords: Medicine ; Oncology ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Use of Biguanides to Improve Response to Chemotherapy -- Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival -- Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy -- Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer -- Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF -- Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers -- Prostate Cancer Detection Using a Non-Invasive Method for Quantifying mi RNAs -- DNA Methylation Analysis of Steroid Hormone Receptor Genes -- Control of Oncogenic mi RNA Function by Light-Activated mi RNA Antagomirs -- Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling -- Microvesicles as Mediators of Intercellular Communication in Cancer -- In Vivo Rat Model to Study Horizontal Tumor Progression -- An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice -- Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis -- Zebrafish-Based Systems Pharmacology of Cancer Metastasis -- A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells -- Gene Disruption Using Zinc Finger Nuclease Technology
    Abstract: Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study.℗ Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment
    Pages: XI, 263 p. 42 illus., 27 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493908561
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