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  • 1
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    New York. : Humana Press
    Associated volumes
    Call number: QH506:60/514(2)
    Keywords: T-Lymphocytes / immunology
    Pages: xi, 165 p : ill.
    Edition: 2nd ed.
    ISBN: 9781588295873
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  • 2
    Call number: QH506:60/473
    Keywords: Epidemiology ; Epidemiology / Methodology
    Pages: x, 321 p. : ill
    ISBN: 9781588299642
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  • 3
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    Totowa, N.J. : Humana Press
    Associated volumes
    Call number: QH506:60/472
    Keywords: Neoplasms / epidemiology ; Neoplasms / prevention & control
    Pages: xiv, 484 p. : ill.
    ISBN: 9781603274913
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  • 4
    Call number: QH506:60/471
    Keywords: Neoplasms / epidemiology ; Neoplasms / prevention & control
    Pages: xii, 507 p. : ill.
    ISBN: 9781588299871
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  • 5
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Embryology ; Life sciences ; Cell Biology ; Embryology ; Developmental Biology ; Springer eBooks
    Description / Table of Contents: General Aspects of Fertility and Infertility -- Genetics of Male Fertility -- Genetics of Female Infertility due to Anomalies of the Ovary and Mullerian Ducts -- Gene Polymorphisms in Female Reproduction -- Understanding the Spermatozoon -- Derivation of Human Embryonic Stem Cells (hESC) -- The Endocrinology of the Menstrual Cycle -- Assisted Reproductive Techniques -- Novel Markers of Male Infertility -- Luteal Phase Support in ART Treatments -- General Principles of Cryopreservation -- In Vitro Maturation of Immature Human Oocytes for Clinical Application -- GnRH Antagonist Based Protocols for In Vitro Fertilization -- Ovarian Stimulation for IVF: Mild Approaches -- IVF Stimulation: Protocols for Poor Responders -- Oocyte Retrieval and Quality Evaluation -- Sperm Retrieval and Quality Evaluation -- Treatment of Male Infertility -- Techniques for Slow Cryopreservation of Embryos -- Cryopreservation of Eggs -- Ovarian Tissue Cryopreservation and Transplantation. A Realistic, Effective Technology for Fertility Preservation -- Detection of Monogenic Disorders and Chromosome Aberrations by Preimplantation Genetic Diagnosis -- Embryo Culture and Selection: Morphological Criteria -- Embryo Selection using Metabolomics -- Embryo Transfer -- Safety of Assisted Reproductive Technologies -- Human Germ Cell Differentiation from Pluripotent Embryonic Stem Cells and Induced Pluripotent Stem Cells
    Abstract: Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell, and molecular biologists, and others in the biomedical sciences. The volume presents straight-forward manner best practice approaches for overcoming a host of fertility challenges.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ ℗ Authoritative and cutting-edge, Human Fertility: Methods and Protocols aids scientists in continuing to study assisted reproductive technologies
    Pages: XI, 584 p. 115 illus., 87 illus. in color. : online resource.
    ISBN: 9781493906598
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  • 6
    Keywords: Chemistry ; Gene Expression ; Biotechnology ; Chemistry ; Biotechnology ; Gene Expression ; Springer eBooks
    Description / Table of Contents: An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae -- Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae -- Tools for Genetic Engineering of the Yeast Hansenula polymorpha -- Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory -- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris -- Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains -- Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries -- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology -- Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach -- Evolutionary Engineering of Yeast -- Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains -- Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.-℗ 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case -- Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis -- QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast -- Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications -- Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae -- Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace
    Abstract: Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha, and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail, as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume.℗ Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies
    Pages: X, 316 p. 44 illus., 18 illus. in color. : online resource.
    ISBN: 9781493905638
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  • 7
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Efficient Library Preparation for Next-Generation Sequencing Analysis of Genome-Wide Epigenetic and Transcriptional Landscapes in Embryonic Stem Cells -- Analysis of Next-Generation Sequencing Data Using Galaxy -- edgeR for Differential RNA-seq℗ and ChIP-seq Analysis: An Application℗ to Stem℗ Cell Biology -- Use Model-based Analysis of ChIP-Seq (MACS) to Analyze Short Reads Generated by Sequencing Protein-DNA Interactions in Embryonic Stem Cells -- Spatial Clustering for Identification of ChIP-Enriched Regions (SICER) to Map Regions of Histone Methylation Patterns in Embryonic Stem Cells -- Identifying Stem Cell Gene Expression Patterns and Phenotypic Networks with AutoSOME -- Visualization and Clustering of High-Dimensional Transcriptome Data Using GATE -- Interpreting and Visualizing ChIP-seq Data with the seqMINER Software -- A Description of the Molecular Signatures Database (MSigDB) Website -- Use of Genome-Wide RNAi Screens to Identify Regulators of Embryonic Stem Cell Self-Renewal and Pluripotency -- Correlating Histone Modification Patterns with Gene Expression Data During Hematopoiesis -- In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes and Preimplantation Embryo Culture -- Derivation and Manipulation of Trophoblast Stem Cells from Mouse Blastocysts -- Conversion of Epiblast Stem Cells to Embryonic Stem Cells Using Growth Factors and Small Molecule Inhibitors -- Generation of Induced Pluripotent Stem Cells Using Chemical Inhibition and Three Transcription Factors -- Transdifferentiation of Mouse Fibroblasts and Hepatocytes to Functional Neurons -- Direct Lineage Conversion of Pancreatic Exocrine to Endocrine Beta-Cells In Vivo with Defined Factors -- Direct Reprogramming of Cardiac Fibroblasts to Cardiomyocytes Using MicroRNAs -- Reprogramming Somatic Cells into Pluripotent Stem Cells Using miRNAs
    Abstract: Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis.℗ Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation.℗ Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks
    Pages: X, 286 p. 150 illus., 142 illus. in color. : online resource.
    ISBN: 9781493905126
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  • 8
    Keywords: Medicine ; Medical laboratories ; Medical virology ; Emerging infectious diseases ; Medicine & Public Health ; Infectious Diseases ; Virology ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
    Abstract: Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research
    Pages: XIII, 403 p. 82 illus., 49 illus. in color. : online resource.
    ISBN: 9781493903481
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  • 9
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Simultaneous Functional Magnetic Resonance and Two Dimensional Optical Imaging Spectroscopy -- Neurovascular Coupling Investigated by Simultaneous Optical Coherence Tomography and Electrophysiology -- Studying Adenosine and Cerebral Blood Flow (CBF) Using Pharmacological Methods -- Imaging Vasodynamics in the Awake Mouse Brain with Two-Photon Microscopy -- Neurovascular Coupling in the Deep Brain Using Confocal Fiber-Optic Endomicroscopy -- Non-Invasive Neural Imaging and Tissue Oxygenation in the Visual System -- High-Resolution Wide-Field Optical Imaging of Micro-Vascular Characteristics: From the Neocortex to the Eye -- Optical Intrinsic Signal Imaging for Elucidating Functional Structures in Higher Visual Area -- Closed Cranial Window Applications in the Rat: Studies on Neurovascular Coupling Involving Pial Arterioles and the Glia Limitans -- Multi-Spectral Imaging of Blood Volume, Metabolism, Oximetry and Light Scattering -- Physiological Basis of BOLD fMRI Decreases -- Simultaneous Multi-Wavelength Optical Imaging of Neuronal and Hemodynamic Activity -- Non-Invasive Laser-Induced Photoacoustic Tomography for Functional Imaging -- Neurovascular Coupling in Relation to Cortical Spreading Depression -- Cerebral Blood Flow Imaging with Laser Speckle Contrast Imaging -- Cerebral Monitoring and Surveillance Using High Resolution Functional Optical Imaging
    Abstract: Neurovascular Coupling Methods brings the reader up to date with the current state-of-the-art techniques in measuring blood flow in the brain, with chapters describing different techniques, or combinations of techniques, applied to specific species in either healthy or abnormal brains. Opening with a section on techniques in normal somatosensory processing, the detailed volume continues with parts covering techniques in normal visual processing, epilepsy, and cerebrovascular diseases, such as ischemia, hemorrhage, and spreading depression.℗ As a collection in the popular Neuromethods series, this book contains the kind of thorough description and implementation advice that will lead to successful results in the lab. ℗ Authoritative and practical, Neurovascular Coupling Methods serves as an ideal guide for researchers aiming to further our knowledge of these coupling mechanisms in the hopes of ultimately understanding higher order functions such as language and memory and developing novel brain mapping techniques that can be employed in humans
    Pages: XIX, 332 p. 120 illus., 99 illus. in color. : online resource.
    ISBN: 9781493907243
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  • 10
    Keywords: Chemistry ; Biotechnology ; Biochemical engineering ; Chemistry ; Biotechnology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Recent Progress in Engineering Human-associated Microbiomes -- Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis -- Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species -- Design, Construction and Characterization Methodologies for Synthetic Microbial Consortia -- An Observation Method for Autonomous Signaling-mediated Synthetic Diversification in Escherichia coli -- Integration-free Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPSCs) without Viral Vectors, Recombinant DNA and Genetic Modification -- Transformation of Bacillus subtilis -- Culturing Anaerobes to use as a Model System for Studying the Evolution of Syntrophic Mutualism -- Therapeutic Microbes for Infectious Disease -- Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System -- Transcriptome Analysis of a Microbial Coculture in which the Cell Populations are Separated by a Membrane -- Identification of Mutations in Laboratory Evolved Microbes from Next-generation Sequencing data using breseq -- 3D-Fluorescence in situ Hybridization of Intact, Anaerobic Biofilm -- The Characterization of Living Bacterial Colonies Using Nano spray Desorption Electrospray Ionization Mass Spectrometry -- Modeling Community Population Dynamics with the Open-Source Language R -- Simulating Microbial Community Patterning using Biocellion
    Abstract: Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Engineering Multicellular Systems: Methods and Protocols provides a comprehensive laboratory protocol reference for constructing multicellular systems for various applications
    Pages: XI, 256 p. 109 illus., 86 illus. in color. : online resource.
    ISBN: 9781493905546
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