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  • 1
    Call number: QH506:60/485(2)
    Keywords: HIV (Viruses) / Research ; HIV (Viruses)
    Pages: xiv, 457 p. : ill.
    Edition: 2nd ed.
    ISBN: 9781588298591
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  • 2
    Keywords: Life sciences ; Bioinformatics ; Biological models ; Biology / Data processing ; Life sciences ; Bioinformatics ; Systems Biology ; Computer Appl. in Life Sciences ; Springer eBooks
    Description / Table of Contents: Introduction to Biomedical literature text mining: Context and Objectives -- Accessing Biomedical Literature in the Current Information Landscape -- Mapping of Biomedical Text to Concepts of Lexicons, Terminologies and Ontologies -- Drug Interaction Text Mining -- Biological Information Extraction and Co-occurence Analysis: State of the Art and Perspectives -- Roles of Text Mining in Protein Function Prediction -- Functional Molecular Units for Guiding Biomarker Panel Design -- Mining Biological Networks from Full-text Articles -- Scientific Collaboration Networks using Biomedical Text -- Predicting future discoveries from current scientific literature -- Mining Emerging Biomedical Literature for Understanding Disease Associations in Drug Discovery -- Integrating Literature and Data Mining to Rank Disease Candidate Genes -- Role of Text Mining in Early Identification of Potential Drug Safety Issues -- Systematic Drug Repositioning using Text Mining -- Mining℗ the Electronic Health Record for Disease-Specific Knowledge
    Abstract: Biomedical Literature Mining,℗ discusses the multiple facets of modern biomedical literature mining and its many applications in genomics and systems biology. The volume is divided into three sections focusing on information retrieval, integrated text-mining approaches, and domain-specific mining methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Biomedical Literature Mining℗ is℗ designed as a useful bioinformatics resource in biomedical literature text mining for both those long experienced in, or entirely new to, the field
    Pages: XII, 288 p. 51 illus., 36 illus. in color. : online resource.
    ISBN: 9781493907090
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  • 3
    Keywords: Medicine ; Immunology ; Vaccines ; Genetic Engineering ; Biomedicine ; Immunology ; Genetic Engineering ; Vaccine ; Springer eBooks
    Description / Table of Contents: A Blueprint for DNA Vaccines Design -- Enhancement of Plasmid-Mediated Transgene Expression -- Strategies for Improving DNA Vaccines Performance -- Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies -- Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies -- Development of Antibiotic-Free Selection System for Safer DNA Vaccination -- Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin -- DNA Vaccination in Skin Enhanced by Electroporation -- Intradermal Vaccination by DNA Tattooing -- Microneedle Applications for DNA Vaccine Delivery to the Skin -- Multivalent DNA-Based Vectors for DNA Vaccines Delivery -- Superparamagnetic Nanoparticles Delivery of DNA Vaccine -- Plasmid Fermentation Process for DNA Immunization Applications -- Pharmaceutical Grade℗ Large Scale℗ Plasmid DNA Manufacturing Process -- Protective and Therapeutic DNA Vaccination Against Allergic Diseases -- Immunotherapy for Alzheimeŕ€™s Disease: DNA- and Protein-Based Epitope Vaccines -- Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue -- DNA Vaccination as a Treatment for Chronic Kidney Disease
    Abstract: DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.℗ Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.℗ Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease
    Pages: XI, 308 p. 43 illus., 32 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493904105
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  • 4
    Keywords: Medicine ; Orthopedics ; Rheumatology ; Biomedicine ; Molecular Medicine ; Orthopedics ; Rheumatology ; Springer eBooks
    Description / Table of Contents: Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction -- Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients -- Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors -- Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice -- Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice -- In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets -- Characterization of microRNAs and Their Targets -- Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell -- Basic Techniques for Studies of iNKT Cells and MAIT Cells -- Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen -- Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis -- Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach -- Genome-Wide Genetic Study in Autoimmune Disease Prone Mice -- Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis -- Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples -- Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy
    Abstract: Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field
    Pages: X, 204 p. 65 illus., 31 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493904044
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  • 5
    Keywords: Life sciences ; Agriculture ; Botany ; Life sciences ; Plant Sciences ; Agriculture ; Springer eBooks
    Description / Table of Contents: Measuring Freezing Tolerance: Survival and Regrowth Assays -- Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays -- Conducting Field Trials for Freezing Tolerance Breeding in Cereals -- A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance -- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation -- Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions -- Identification of Arabidopsis Mutants with Altered Freezing Tolerance -- Infrared Thermal Analysis of Plant Freezing Processes -- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues -- Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images -- Proteomic Approaches to Identify Cold Regulated Soluble Proteins -- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins -- Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry -- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition -- Quantification of Superoxide and Hydrogen Peroxide in Leaves -- Estimating Ice Encasement Tolerance of Herbage Plants -- Characterization of Ice Binding Proteins from Sea Ice Algae -- Isolation and Characterization of Ice Binding Proteins from Higher Plants.℗
    Abstract: Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties.℗ Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches
    Pages: XI, 282 p. 75 illus., 49 illus. in color. : online resource.
    ISBN: 9781493908448
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  • 6
    Keywords: Life sciences ; Food science ; Microbiology ; Life sciences ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Sampling the Production/Processing Environment for Listeria -- Traditional Methods for Isolation of Listeria monocytogenes -- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR -- Serotype Assignment by Sero-Agglutination, ELISA and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes -- Ribotyping and Automated Ribotyping of Listeria monocytogenes -- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- Analysis of Listeria monocytogenes Subproteomes -- The Listeria Cell Wall and Associated Carbohydrate Polymers -- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria -- Virulence Characterization of Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method -- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes -- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR -- Genome Sequencing of Listeria monocytogenes -- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels -- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers -- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments
    Abstract: Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and, finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research
    Pages: X, 286 p. 42 illus., 17 illus. in color. : online resource.
    ISBN: 9781493907038
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  • 7
    Keywords: Chemistry ; Biotechnology ; Biochemical engineering ; Chemistry ; Biotechnology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Recent Progress in Engineering Human-associated Microbiomes -- Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis -- Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species -- Design, Construction and Characterization Methodologies for Synthetic Microbial Consortia -- An Observation Method for Autonomous Signaling-mediated Synthetic Diversification in Escherichia coli -- Integration-free Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPSCs) without Viral Vectors, Recombinant DNA and Genetic Modification -- Transformation of Bacillus subtilis -- Culturing Anaerobes to use as a Model System for Studying the Evolution of Syntrophic Mutualism -- Therapeutic Microbes for Infectious Disease -- Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System -- Transcriptome Analysis of a Microbial Coculture in which the Cell Populations are Separated by a Membrane -- Identification of Mutations in Laboratory Evolved Microbes from Next-generation Sequencing data using breseq -- 3D-Fluorescence in situ Hybridization of Intact, Anaerobic Biofilm -- The Characterization of Living Bacterial Colonies Using Nano spray Desorption Electrospray Ionization Mass Spectrometry -- Modeling Community Population Dynamics with the Open-Source Language R -- Simulating Microbial Community Patterning using Biocellion
    Abstract: Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Engineering Multicellular Systems: Methods and Protocols provides a comprehensive laboratory protocol reference for constructing multicellular systems for various applications
    Pages: XI, 256 p. 109 illus., 86 illus. in color. : online resource.
    ISBN: 9781493905546
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  • 8
    Keywords: Life sciences ; Microbiology ; Bacteriology ; Life sciences ; Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Gene Transfer: Transduction -- Gene Transfer: Transformation/Electroporation -- Gene transfer: Conjugation -- Pseudomonas Bacteriophage Isolation and Production -- Genotyping Methods -- Drug Susceptibility Testing by Dilution Methods -- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa -- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa -- Motility Assay: Twitching Motility -- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis -- Microscopic Analysis: Morphotypes and Cellular Appendages -- Determination of Lipolytic Activities -- Elastinolytic and proteolytic enzymes -- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins -- Cell Fractionation -- Characterization of Molecular Interactions using Isothermal Titration Calorimetry -- Proteomic Analysis -- Membrane Proteomics of Pseudomonas aeruginosa -- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays -- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules -- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules -- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP -- Metabolic Footprinting: Extracellular Metabolomic Analysis -- Pyoverdine and Pyochelin Measurements -- Measurement of Phenazines in Bacterial Cultures -- Extraction and Measurement of NAD(P)+ and NAD(P)H -- Cyanide Measurements in Bacterial Culture and Sputum -- Monitoring Iron Uptake by Siderophores -- Exopolysaccharide Quantification -- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids -- LPS quantitation Procedures -- Monitoring Lectin Interaction with Carbohydrates -- Mining the Pseudomonas Genome -- Identification of Bacterial Small RNAs by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis -- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics -- Signature Tagged Mutagenesis -- Construction of a Pseudomonas aeruginosa Genomic Bank -- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes -- Promoter Fusions with Optical Outputs in Individual Cells and in Populations -- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq -- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans -- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop -- Biofilm Formation in the 96-well Microtiter Plate -- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa -- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans -- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum -- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella -- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana -- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells -- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish -- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster -- Assessing Pseudomonas Virulence using Host Cells -- Assessing Pseudomonas aeruginosa℗ Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection -- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model -- Burn Mouse Models
    Abstract: In℗ Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings
    Pages: XVIII, 816 p. 157 illus., 89 illus. in color. : online resource.
    ISBN: 9781493904730
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  • 9
    Keywords: Medicine ; Oncology ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Use of Biguanides to Improve Response to Chemotherapy -- Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival -- Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy -- Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer -- Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF -- Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers -- Prostate Cancer Detection Using a Non-Invasive Method for Quantifying mi RNAs -- DNA Methylation Analysis of Steroid Hormone Receptor Genes -- Control of Oncogenic mi RNA Function by Light-Activated mi RNA Antagomirs -- Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling -- Microvesicles as Mediators of Intercellular Communication in Cancer -- In Vivo Rat Model to Study Horizontal Tumor Progression -- An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice -- Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis -- Zebrafish-Based Systems Pharmacology of Cancer Metastasis -- A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells -- Gene Disruption Using Zinc Finger Nuclease Technology
    Abstract: Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study.℗ Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment
    Pages: XI, 263 p. 42 illus., 27 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493908561
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  • 10
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Axon Regeneration: What Needs to Be Overcome? -- Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro -- The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance Related Intracellular Signaling in Live Dorsal Root Ganglia Neuronal Growth Cones -- Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues -- Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants -- Growth Cone Collapse Assay -- Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study -- Organotypic Slice Co-Culture Systems to Study Axon Regeneration in the Dopaminergic System Ex Vivo -- Monitoring Neuron and Astrocyte Interactions with a 3D Cell Culture System -- Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury -- Polymeric Biomaterials for Nerve Regeneration: Fabrication and Implantation of a Biodegradable Nerve Guide -- A Highly Reproducible Mouse Model of Compression Spinal Cord Injury -- Using Templated Agarose Scaffolds to Promote Axon Regeneration Through Sites of Spinal Cord Injury -- In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration -- Assessing Motor Outcome and Functional Recovery Following Nerve Injury -- The Use of an Adeno-Associated Viral Vector for Efficient Bicistronic Expression of Two Genes in the Central Nervous System -- Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues -- Time-Lapse In Vivo Imaging of Dorsal Root Nerve Regeneration in Mice -- Using Manganese-Enhanced MRI to Assess Optic Nerve Regeneration
    Abstract: Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies.℗ After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors, and methods for quantifying outgrowth. The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery. As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field
    Pages: XI, 253 p. 66 illus., 53 illus. in color. : online resource.
    ISBN: 9781493907779
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