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1.
PAPER CURRENT
Modulation of Ion Channels by Cysteine-Rich Peptides: From Sequence to Structure (2017)
Elsevier
Publication Date: 2017-04-26
Description: Publication date: Available online 24 April 2017 Source: Advances in Pharmacology Author(s): Mehdi Mobli, Eivind A.B. Undheim, Lachlan D. Rash Venom peptides are natural ligands of ion channels and have been used extensively in pharmacological characterization of various ion channels and receptors. In this chapter, we survey all known venom peptide ion-channel modulators. Our survey reveals that the majority of venom peptides characterized to date target voltage-gated sodium or potassium channels. We further find that the majority of these peptides are found in scorpion and spider venoms. We discuss the influence of the pharmacological tools available in biasing discovery and the classical “toxin-to-sequence” approach to venom peptide biodiscovery. The impact of high-throughput sequencing on the existing discovery framework is likely to be significant and we propose here an alternative “sequence-to-toxin” approach to peptide screening, relying more on recently developed high-throughput methods. Methods for production and characterization of disulfide rich toxins in a high-throughput setting are then described, focusing on bacterial protein expression and solution state structural characterization by NMR spectroscopy. Finally, the role of X-ray crystallography and cryo-EM are highlighted by discussing the currently known channel-peptide complexes.
Print ISSN: 1054-3589
Electronic ISSN: 1557-8925
Topics: Medicine
Published by Elsevier
2.
PAPER CURRENT
Role of Nonneuronal TRPV4 Signaling in Inflammatory Processes (2017)
Elsevier
Publication Date: 2017-04-26
Description: Publication date: Available online 24 April 2017 Source: Advances in Pharmacology Author(s): Pradeep Rajasekhar, Daniel P. Poole, Nicholas A. Veldhuis Transient receptor potential (TRP) ion channels are important signaling components in nociceptive and inflammatory pathways. This is attributed to their ability to function as polymodal sensors of environmental stimuli (chemical and mechanical) and as effector molecules in receptor signaling pathways. TRP vanilloid 4 (TRPV4) is a nonselective cation channel that is activated by multiple endogenous stimuli including shear stress, membrane stretch, and arachidonic acid metabolites. TRPV4 contributes to many important physiological processes and dysregulation of its activity is associated with chronic conditions of metabolism, inflammation, peripheral neuropathies, musculoskeletal development, and cardiovascular regulation. Mechanosensory and receptor- or lipid-mediated signaling functions of TRPV4 have historically been attributed to central and peripheral neurons. However, with the development of potent and selective pharmacological tools, transgenic mice and improved molecular and imaging techniques, many new roles for TRPV4 have been revealed in nonneuronal cells. In this chapter, we discuss these recent findings and highlight the need for greater characterization of TRPV4-mediated signaling in nonneuronal cell types that are either directly associated with neurons or indirectly control their excitability through release of sensitizing cellular factors. We address the integral role of these cells in sensory and inflammatory processes as well as their importance when considering undesirable on-target effects that may be caused by systemic delivery of TRPV4-selective pharmaceutical agents for treatment of chronic diseases. In future, this will drive a need for targeted drug delivery strategies to regulate such a diverse and promiscuous protein.
Print ISSN: 1054-3589
Electronic ISSN: 1557-8925
Topics: Medicine
Published by Elsevier
3.
PAPER CURRENT
Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications (2017)
Springer
Publication Date: 2017-04-26
Description: Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing S...
Electronic ISSN: 2191-0855
Topics: Biology
Published by Springer
4.
PAPER CURRENT
Publication Date: 2017-04-26
Description: Analytical Chemistry DOI: 10.1021/acs.analchem.7b00017
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
5.
PAPER CURRENT
Direct Non-Oxidative Conversion of Methane to Hydrogen and Higher Hydrocarbons by Dielectric Barrier Discharge Plasma with Plasma Catalysis Promoters (2017)
Wiley-Blackwell
Publication Date: 2017-04-26
Description: Direct non-oxidative conversion of methane to hydrogen and hydrocarbons were achieved at atmospheric pressure and 120°C using non-thermal plasma sustained by Plasma Catalysis Promoters (PCPs). Reactors had two different electrode configurations. Methane conversion correlated well with the Specific Energy Density (SED). Methane conversion was independent of plasma power, flow rate, electrode configuration or the type of PCPs. Hydrogen selectivity (ca. 60%) was dependent significantly on PCP and electrode configuration. The ethane/ethylene molar ratio increased from 0 to 0.15 with increasing SED. When the SED value was below ca. 100 kJ/L, ethylene was the only C 2 hydrocarbon. These results are similar to the recently reported non-oxidative catalytic methane conversion at ca. 1000°C. Therefore, these results represents Process Intensification in methane conversion. PCPs underwent structural and chemical changes but their performance are not affected during an 80-hour experimental period. This article is protected by copyright. All rights reserved.
Print ISSN: 0001-1541
Electronic ISSN: 1547-5905
Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
6.
PAPER CURRENT
In Situ Underwater Laser-Induced Breakdown Spectroscopy Analysis for Trace Cr(VI) in Aqueous Solution Supported by Electrosorption Enrichment and a Gas-Assisted Localized Liquid Discharge Apparatus (2017)
American Chemical Society (ACS)
Publication Date: 2017-04-26
Description: Analytical Chemistry DOI: 10.1021/acs.analchem.7b00629
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
7.
PAPER CURRENT
Influences of Normalization Method on Biomarker Discovery in Gas Chromatography–Mass Spectrometry-Based Untargeted Metabolomics: What Should Be Considered? (2017)
American Chemical Society (ACS)
Publication Date: 2017-04-26
Description: Analytical Chemistry DOI: 10.1021/acs.analchem.6b05152
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology
8.
PAPER CURRENT
Validating Granular Segregation Rate Models (2017)
Wiley-Blackwell
Publication Date: 2017-04-26
Print ISSN: 0001-1541
Electronic ISSN: 1547-5905
Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
9.
PAPER CURRENT
Characterization of binding and quantification of human autoantibodies to PDGFRα using a biosensor-based approach (2017)
Elsevier
Publication Date: 2017-04-26
Description: Publication date: Available online 24 April 2017 Source: Analytical Biochemistry Author(s): Gianluca Moroncini, Massimiliano Cuccioloni, Matteo Mozzicafreddo, Katarzyna Natalia Pozniak, Antonella Grieco, Chiara Paolini, Cecilia Tonnini, Tatiana Spadoni, Silvia Svegliati, Ada Funaro, Mauro Angeletti, Armando Gabrielli Systemic sclerosis (SSc) is a chronic autoimmune disease of the connective tissue. The variety and clinical relevance of autoantibodies in SSc patients have been extensively studied, eventually identifying agonistic autoantibodies targeting the platelet-derived growth factor receptor alpha (PDGFRα), and representing potential biomarkers for SSc. We used a resonant mirror biosensor to characterize the binding between surface-blocked PDGFRα and PDGFRα-specific recombinant human monoclonal autoantibodies (mAbs) produced by SSc B cells, and detect/quantify serum autoimmune IgG with binding characteristics similar to the mAbs. Kinetic data showed a conformation-specific, high-affinity interaction between PDGFRα and mAbs, with equilibrium dissociation constants in the low-to-high nanomolar range. When applied to total serum IgG, the assay discriminated between SSc patients and healthy controls, and allowed the rapid quantification of autoimmune IgG in the sera of SSc patients, with anti-PDGFRα IgG falling in the range 3.20–4.67 neq/L of SSc autoantibodies. The test was validated by comparison to direct and competitive anti-PDGFRα antibody ELISA. This biosensor assay showed higher sensibility with respect to ELISA, and other major advantages such as the specificity, rapidity, and reusability of the capturing surface, thus representing a feasible approach for the detection and quantification of high affinity, likely agonistic, SSc-specific anti-PDGFRα autoantibodies. Graphical abstract
Print ISSN: 0003-2697
Electronic ISSN: 1096-0309
Topics: Biology , Chemistry and Pharmacology
Published by Elsevier
10.
PAPER CURRENT
On the necessity of translational cognitive-neurotoxicological research in methamphetamine abuse and addiction (2017)
Springer
Publication Date: 2017-04-26
Print ISSN: 0340-5761
Electronic ISSN: 1432-0738
Topics: Medicine
Published by Springer