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1.
PAPER CURRENT
Series Page (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: 2017 Source: Advances in Genetics, Volume 100
Print ISSN: 0065-2660
Topics: Biology
Published by Elsevier
2.
PAPER CURRENT
Publication Date: 2017-12-17
Description: Publication date: 2017 Source: Advances in Genetics, Volume 100 Author(s): Jeffrey P. Townsend, Zheng Wang
Print ISSN: 0065-2660
Topics: Biology
Published by Elsevier
3.
PAPER CURRENT
Publication Date: 2017-12-17
Description: Publication date: January 2018 Source: The American Journal of Pathology, Volume 188, Issue 1
Print ISSN: 0002-9440
Electronic ISSN: 1525-2191
Topics: Medicine
4.
PAPER CURRENT
Publication Date: 2017-12-17
Description: Publication date: January 2018 Source: The American Journal of Pathology, Volume 188, Issue 1
Print ISSN: 0002-9440
Electronic ISSN: 1525-2191
Topics: Medicine
5.
PAPER CURRENT
Malnutrition in pancreatic ductal adenocarcinoma (PDA): dietary pancreatic enzymes improve short-term health but stimulate tumor growth (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: Available online 15 December 2017 Source: The American Journal of Pathology Author(s): Yalda Zolghadri, Shreoshi Pal Choudhuri, Ozhan Ocal, Somayeh Layeghi- Ghalehsoukhteh, Feaven Berhe, Michael A. Hale, Thomas M. Wilkie Pancreatic ductal adenocarcinoma (PDA) is a deadly cancer that resists efforts to identify better chemotherapeutics. PDA is associated with chronic pancreatitis and acinar cell dedifferentiation. This reduces enzyme production by the exocrine pancreas, resulting in digestive insufficiencies. Malabsorption of partially digested food can cause bloating, overfilled intestines, abdominal pain, excessive feces, steatorrhea, and malnutrition. These maladies affect quality of life and restrict treatment options for pancreatitis and PDA. Here, we characterize the health benefits and risks of dietary pancreatic enzymes in three mouse models of PDA—KC, KCR8-16, and KIC. KC expresses oncogenic Kras G12D in pancreatic tissue whereas KCR8-16 also has deletions of the Rgs8 and Rgs16 genes. Rgs proteins inhibit the release of digestive enzymes evoked by G-protein coupled receptor agonists. KC and KCR8-16 mice developed dedifferentiated exocrine pancreata within two months of age, and became malnourished, underweight, hypoglycemic, and hypothermic. KC mice adapted but KCR8-16 rapidly transitioned to starvation after mild metabolic challenges. Dietary pancreatic enzyme supplements reversed these symptoms in KC and KCR8-16 animals, and extended survival. Therefore, we tested benefits of pancreatic enzymes in an aggressive mouse model of PDA (KIC). Median survival improved with dietary pancreatic enzyme supplements and was further extended when combined with warfarin and gemcitabine chemotherapy. However, dietary pancreatic enzymes stimulated tumor growth in the terminal stages of disease progression in KIC mice.
Print ISSN: 0002-9440
Electronic ISSN: 1525-2191
Topics: Medicine
6.
PAPER CURRENT
Discrete pools of oligomeric amyloid-β track with spatial learning deficits in a mouse model of Alzheimer amyloidosis (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: Available online 15 December 2017 Source: The American Journal of Pathology Author(s): Angie C.A. Chiang, Stephanie W. Fowler, Rohit Reddy, Olga Pletnikova, Juan C. Troncoso, Mathew A. Sherman, Sylvain E. Lesne, Joanna L. Jankowsky Despite increasing appreciation that oligomeric amyeloid beta (Aβ) may contribute to cognitive decline of Alzheimer disease, defining the most critical forms has been thwarted by the changeable nature of these aggregates and the varying methods used for detection. Here, using a broad approach, we quantified Aβ oligomers during the evolution of cognitive deficits in an aggressive model of Aβ amyloidosis. Amyloid precursor protein/tetracycline transactivator mice underwent behavioral testing at 3, 6, 9, and 12 months of age to evaluate spatial learning and memory, followed by histological assessment of amyloid burden and biochemical characterization of oligomeric Aβ species. Transgenic mice displayed progressive impairments in acquisition and immediate recall of the trained platform location. Biochemical analysis of cortical extracts from behaviorally tested mice revealed distinct age-dependent patterns of accumulation in mulitple oligomeric species. Dot blot analysis demonstrated that non-fibrillar Aβ oligomers (A11) were highly soluble and extracted into a fraction enriched for extracellular proteins, whereas pre-fibrillar (OC) species required high-detegerent conditions to retrieve, consistent with membrane localization. Low-detergent extracts tested by 82E1 ELISA confirmed the presence of bona fide Aβ oligomers, whereas immunoprecipitation-Western using high-detergent extracts revealed a variety of SDS-stable low-n species. These findings show that different Aβ oligomers vary in solubility consistent with distinct localization, and identify A11-positive non-fibrillar aggregates as tracking most closely with cognitive decline in this model.
Print ISSN: 0002-9440
Electronic ISSN: 1525-2191
Topics: Medicine
7.
PAPER CURRENT
Voltammetric determination of thujone in herbal matrices in the presence of Triton X-100 (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: 15 February 2018 Source: Analytical Biochemistry, Volume 543 Author(s): Mateusz Kowalcze, Małgorzata Jakubowska A simple, sensitive and rapid method of low concentration of thujone determination using Controlled Growth Mercury Drop Electrode and Differential Pulse Voltammetry was described. The reduction of thujone in phosphoric acid or potassium nitrate, involves a quasi-reversible and adsorption-controlled two-electron process. In voltammetric experiments the addition of ethanol and Triton X–100 is recommended. Experimental conditions, such as composition of supporting electrolyte, concentration of Triton X-100 and operation parameters were established. The optimal electrolyte should contain: 0.04 mol L −1 phosphoric acid (pH = 1.9) or 0.05 mol L −1 potassium nitrate (pH = 6.4), 0.0002% Triton X-100 and 9.6% ethanol. The calibration graph was linear from 0.7 to 140 mgL −1 of thujone in H 3 PO 4 as supporting electrolyte and from 0.7 to 115 mgL −1 of the analyte in KNO 3 . The detection limit was 0.4 mgL −1 in H 3 PO 4 and 0.6 mgL −1 in KNO 3 , with the correlation coefficient of 0.998. The influence of interfering substances, such as Al(III), Bi(III), Cd(II), Cr(III), Cu(II), Pb(II), Tl(I), V(III) and Zn(II) was studied. The proposed method was validated by the thujone recovery study from the specially prepared spiked herbal matrices and alcohol extract. The considered real sample was thuja oil, in which thujone was successfully determined. All measurements were performed without sample preparation.
Print ISSN: 0003-2697
Electronic ISSN: 1096-0309
Topics: Biology , Chemistry and Pharmacology
Published by Elsevier
8.
PAPER CURRENT
Evaluation of digestion methods for analysis of trace metals in mammalian tissues and NIST 1577c (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: 15 February 2018 Source: Analytical Biochemistry, Volume 543 Author(s): Grace A. Binder, Rainer Metcalf, Zachary Atlas, Kenyon G. Daniel Digestion techniques for ICP analysis have been poorly studied for biological samples. This report describes an optimized method for analysis of trace metals that can be used across a variety of sample types. Digestion methods were tested and optimized with the analysis of trace metals in cancerous as compared to normal tissue as the end goal. Anthropological, forensic, oncological and environmental research groups can employ this method reasonably cheaply and safely whilst still being able to compare between laboratories. We examined combined HNO 3 and H 2 O 2 digestion at 170 °C for human, porcine and bovine samples whether they are frozen, fresh or lyophilized powder. Little discrepancy is found between microwave digestion and PFA Teflon pressure vessels. The elements of interest (Cu, Zn, Fe and Ni) yielded consistently higher and more accurate values on standard reference material than samples heated to 75 °C or samples that utilized HNO 3 alone. Use of H 2 SO 4 does not improve homogeneity of the sample and lowers precision during ICP analysis. High temperature digestions (>165 °C) using a combination of HNO 3 and H 2 O 2 as outlined are proposed as a standard technique for all mammalian tissues, specifically, human tissues and yield greater than 300% higher values than samples digested at 75 °C regardless of the acid or acid combinations used. The proposed standardized technique is designed to accurately quantify potential discrepancies in metal loads between cancerous and healthy tissues and applies to numerous tissue studies requiring quick, effective and safe digestions.
Print ISSN: 0003-2697
Electronic ISSN: 1096-0309
Topics: Biology , Chemistry and Pharmacology
Published by Elsevier
9.
PAPER CURRENT
Real-time RPA assay for rapid detection and differentiation of wild-type pseudorabies and gE-deleted vaccine viruses (2017)
Elsevier
Publication Date: 2017-12-17
Description: Publication date: 15 February 2018 Source: Analytical Biochemistry, Volume 543 Author(s): Jianchang Wang, Libing Liu, Jinfeng Wang, Xiaoyu Pang, Wanzhe Yuan The objective of this study was to develop a dual real-time recombinase polymerase amplification (RPA) assay using exo probes for the detection and differentiation of pseudorabies virus (PRV). Specific RPA primers and probes were designed for gB and gE genes of PRV within the conserved region of viral genome. The reaction process can be completed in 20 min at 39 °C. The dual real-time RPA assay performed in the single tube was capable of specific detecting and differentiating of the wild-type PRV and gE-deleted vaccine strains, without cross-reactions with other non-targeted pig viruses. The analytical sensitivity of the assay was 10 2 copies for gB and gE genes. The dual real-time RPA demonstrated a 100% diagnostic agreement with the real-time PCR on 4 PRV strains and 37 clinical samples. Through the linear regression analysis, the R 2 value of the real-time RPA and the real-time PCR for gB and gE was 0.983 and 0.992, respectively. The dual real-time RPA assay provides an alternative useful tool for rapid, simple, and reliable detection and differentiation of PRV, especially in remote and rural areas.
Print ISSN: 0003-2697
Electronic ISSN: 1096-0309
Topics: Biology , Chemistry and Pharmacology
Published by Elsevier
10.
PAPER CURRENT
Continuous and High-Throughput Electromechanical Lysis of Bacterial Pathogens Using Ion Concentration Polarization (2017)
American Chemical Society (ACS)
Publication Date: 2017-12-17
Description: Analytical Chemistry DOI: 10.1021/acs.analchem.7b03746
Print ISSN: 0003-2700
Electronic ISSN: 1520-6882
Topics: Chemistry and Pharmacology