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  • 1
    Unknown
    Unknown
    Philadelphia : Wolters Kluwer
    Type of Medium: Unknown
    Pages: vii, 668 p. : , ill.
    Edition: 7th ed.
    ISBN: 9781975112516
    Language: English
    Location/Call number: Library / QR360:271(7)/4
    Location/Call number: F035 / F035:014
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  • 2
    E-Resource
    E-Resource
    Cambridge, United Kingdom : Cambridge University Press
    Subject(s): Tumors. ; Oncogenes. ; Nucleotide sequence.
    Description / Table of Contents: "Thoroughly updated with 50% new material, this introductory textbook covers the basic principles and latest research on the causes, growth, detection, and treatment of cancers. Student learning is supported with a stepwise presentation, 'Pause and Recap' boxes, end-of-chapter review questions, and additional online resources"--
    Type of Medium: Electronic Resource
    Pages: 450 p. : , ill.
    Edition: Second edition.
    ISBN: 9781316512616
    Language: English
    Location/Call number: Library / QZ200:598(2)
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  • 3
    Subject(s): Food science. ; Food Science.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details state-of- the art methods on sustainable food extractions. Chapters guide readers on traditional and novel extraction techniques, as well as exploring diverse sources of bioactive compounds. Additionally, chapters provide a holistic view of the field, catering to the needs of researchers, industry professionals, and students who are interested in this rapidly evolving area. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Bioactive Extraction and Application in Food and Nutraceutical Industries aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
    Type of Medium: Online Resource
    Pages: XIV, 473 p. 65 illus., 53 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636015
    Series Statement: Methods and Protocols in Food Science,
    Language: English
    Note: Technologies for Extraction of Bioactive Compounds and its Applications -- Extraction of bioactive and nutraceuticals from plants and their application -- Extraction of bioactive and nutraceuticals from marine sources and their application -- Microwave-assisted extraction of bioactive and nutraceuticals -- Ultrasound Assisted Extraction for Food, Pharmacy and Biotech Industries -- Super and Sub critical fluid extraction of Nutraceuticals and Novel Phytocompound. - Novel Solvent Based Extraction -- Enzyme-Assisted Extraction -- Pulsed Electric Fields as a Green Technology for the Extraction of Bioactive Compounds -- Pulsed Electric Field Extraction -- Case studies and application of Different Novel Extraction Methods -- Pressurized liquid extraction for the isolation of Bioactive Compounds -- Fruit Waste: Potential Bio-resource for Extraction of Nutraceuticals and Bioactive Compounds -- Plant Seeds: A Potential Bio-Resource for Isolation of Nutraceutical and Bioactive Compounds -- Essential Oils: Sustainable Extraction Techniques and Nutraceuticals Perspectives -- Green and Clean Extraction Technologies for Novel Nutraceuticals -- Optimization of Extraction for Nutraceuticals -- Computational approach and its application in the nutraceutical industry.
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  • 4
    Subject(s): Microbiology. ; Bacteria. ; Genomics. ; Microbiology. ; Bacteria. ; Genomics.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
    Type of Medium: Online Resource
    Pages: XII, 418 p. 97 illus., 77 illus. in color. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071635650
    Series Statement: Methods in Molecular Biology, 2741
    Language: English
    Note: RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method -- Extraction and purification of outer membrane vesicles and their associated RNAs -- Analysis of phage regulatory RNAs: sequencing library construction from the fraction of small prokaryotic RNAs less than 50 nucleotides in length -- Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic -- Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus -- CRISPR interference-based functional small RNA genomics -- Investigation of sRNA-mRNA interactions in Bacillus subtilis in vivo -- In vitro methods for the investigation of sRNA-mRNA interactions in Bacillus subtilis -- RNA double helix hybridization measured by fluorescence correlation spectroscopy -- New perspectives on cross talks between bacterial regulatory RNAs from outer membrane vesicles and eukaryotic cells -- Experimental validation of RNA-RNA interactions by Electrophoretic Mobility Shift Assay -- Dynamics and function of sRNA/mRNAs under the scrutiny of computational simulation methods.-Analysis of sRNAs and their mRNA targets in Sinorhizobium meliloti: focus on half-live determinationEvaluation of 5'-end phosphorylation for small RNA stability and target regulation in vivo -- In-gel cyanoethylation for pseudouridines mass spectrometry detection of bacterial regulatory RNA -- Directed screening for sRNA targets in E. coli using a plasmid library -- Defining Bacterial RNA-RNA Interactomes Using CLASH -- Global identification of RNA-binding proteins in bacteria -- An integrated affinity chromatography-based approach to unravel the sRNA interactome in nitrogen-fixing rhizobia -- sRNA structural modeling based on NMR data -- Circular and linear dichroism for the analysis of small noncoding RNA properties.
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  • 5
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Bioinformatics. ; Pharmacology. ; Medicine Research. ; Biology Research. ; Computational and Systems Biology. ; Pharmacology. ; Biomedical Research.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores the application of high-performance computing (HPC) technologies to computational drug discovery (CDD) and biomedicine. The first section collects CDD approaches that, together with HPC, can revolutionize and automate drug discovery process, such as knowledge graphs, natural language processing (NLP), Bayesian optimization, automated virtual screening platforms, alchemical free energy workflows, fragment-molecular orbitals (FMO), HPC-adapted molecular dynamic simulation (MD-HPC), and the potential of cloud computing for drug discovery. The second section delves into computational algorithms and workflows for biomedicine, featuring an HPC framework to assess drug-induced arrhythmic risk, digital patient applications relevant to the clinic, virtual human simulations, cellular and whole-body blood flow modeling for stroke treatments, prediction of the femoral bone strength from CT data, and many more subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary software and tools, step-by-step and readily reproducible modeling protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Performance Computing for Drug Discovery and Biomedicine allows a diverse audience, including computer scientists, computational and medicinal chemists, biologists, clinicians, pharmacologists and drug designers, to navigate the complex landscape of what is currently possible and to understand the challenges and future directions of HPC-based technologies.
    Type of Medium: Online Resource
    Pages: XIII, 429 p. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634493
    Series Statement: Methods in Molecular Biology, 2716
    Language: English
    Note: Introduction to Computational Biomedicine -- Introduction to High Performance Computing -- Computational Biomedicine (CompBioMed) Centre of Excellence: Selected Key Achievements -- In Silico Clinical Trials: Is It Possible? -- Bayesian Optimization in Drug Discovery -- Automated Virtual Screening -- The Future of Drug Development with Quantum Computing -- Edge, Fog, and Cloud Against Disease: The Potential of High-Performance Cloud Computing for Pharma Drug Discovery -- Knowledge Graphs and Their Applications in Drug Discovery -- Natural Language Processing for Drug Discovery Knowledge Graphs: Promises and Pitfalls -- Alchemical Free Energy Workflows for the Computation of Protein-Ligand Binding Affinities -- Molecular Dynamics and Other HPC Simulations for Drug Discovery -- High Throughput Structure-Based Drug Design (HT-SBDD) Using Drug Docking, Fragment Molecular Orbital Calculations, and Molecular Dynamic Techniques -- HPC Framework for Performing In Silico Trials Using a 3D Virtual Human Cardiac Population as Means to Assess Drug-Induced Arrhythmic Risk -- Effect of Muscle Forces on Femur during Level Walking Using a Virtual Population of Older Women -- Cellular Blood Flow Modeling with HemoCell -- A Blood Flow Modeling Framework for Stroke Treatments -- Efficient and Reliable Data Management for Biomedical Applications -- Accelerating COVID-19 Drug Discovery with High-Performance Computing -- Teaching Medical Students to Use Supercomputers: A Personal Reflection.
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  • 6
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurosciences. ; Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest research and development in the areas of Vagus Nerve Stimulation (VNS) as it relates to bioelectronic medicine from neonate to adult. The chapters in this book cover topics such as invasive and non-invasive VNS including methodological considerations (study design, stimulation parameters, and use of heart rate variability metrics); mechanisms of action (automatic regulation and immune plasticity); and disorders where VNS approaches may be therapeutic (migraine and cluster headaches, mood disorders, trauma-related disorders, and language learning). In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Vagus Nerve Stimulation is a valuable resource for both novice and expert preclinical and clinical scientists, clinicians, physicians, and scholars who are interested in learning more about this exciting and developing field.
    Type of Medium: Online Resource
    Pages: XXII, 206 p. 26 illus., 19 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634653
    Series Statement: Neuromethods, 205
    Language: English
    Note: Vagus Nerve Stimulation in Peripheral Targets -- Vagal Nerve Stimulation Through the Lens of the Polyvagal Theory: Recruiting Neurophysiological Mechanisms to Dampen Threat Reactions and Promote Homeostatic Functions -- Heart Rate Variability as a Biomarker for Electrical Vagus Nerve Stimulation -- Vagus Nerve Manipulation and Microglial Plasticity in the Prenatal Brain -- Neonatal Sepsis is Diminished by Cervical Vagus Nerve Stimulation and Tracked Non-Invasively by ECG: A Pilot Report and Dataset in the Piglet Model -- Cognitive Enhancement through Vagus Nerve Stimulation: Methodological Considerations for Behavioral Studies in Rats -- Better Mood through Vagus Nerve Stimulation -- Transcutaneous Vagal Nerve Stimulation in Trauma Spectrum Psychiatric Disorders -- Vagus Nerve Stimulation for Migraine and Cluster Headaches -- Vagus Nerve Stimulation and Language Learning. .
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  • 7
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Allergy. ; Food science. ; Immunology. ; Allergology. ; Food Science.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Food Allergens: Methods and Protocols serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components.
    Type of Medium: Online Resource
    Pages: XII, 377 p. 73 illus., 57 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634530
    Series Statement: Methods in Molecular Biology, 2717
    Language: English
    Note: Food Allergens of Plant and Animal Origin: Classification, Characteristics, and Properties -- Purification of Food Allergens from Their Natural Sources: Chromatographic Methods -- Recombinant Production of Food Allergens in Yeast Pichia pastoris -- Yeast Surface Display Methodology for the Characterization of Food Allergens In Situ -- Characterization of Linear IgE-Binding Epitopes in Food Allergens -- Characterization of T-Cell Epitopes in Food Allergens by Bioinformatic Tools -- Phage Immunoprecipitation Sequencing (PhIP-Seq) for Analyzing Antibody Epitope Repertoires against Food Antigens -- 1D-, 2D-Gel Electrophoresis, Immunoblotting, and Enzyme-Linked Immunosorbent Assay (ELISA) for the Study of Food Allergens -- Preparation of Blinded Food Matrixes for Clinical Oral Challenges -- Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy -- Co-Culture of Human Dendritic and T Cells for the Study of Specific T Cell-Mediated Responses against Food Allergens -- Evaluation of the Suppressive Capacity of Regulatory T Cells in Food Allergy Research -- Mass Cytometry in Food Allergy Research -- Indirect Basophil Activation Test for Peanut Allergy Diagnosis Using Human Donor Basophils -- Standardization of Food Allergen Measurements Using Multiplex Array Technology -- Biosensors for the Detection of Food Allergens -- Standardization of a Mass Spectrometry-Based Workflow for Food Allergen Quantification -- Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph -- Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA) -- Detection of Bet v 1 Homologous Proteins and Plant Profilins by Indirect ELISA -- A Mouse Model of Shrimp Allergy with Cross-Reactivity to Crab and Lobster -- Animal Models in the Study of Food Allergens: Long-Term Maintenance of Allergic Reactivity in Mouse Models of Food Allergy -- Study of MicroRNAs Expression in Food Allergy -- De Novo Transcriptomic Analyses to Identify and Compare Allergens in Foods -- Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research.
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  • 8
    Subject(s): Biological models. ; Biology Technique. ; Biological Models. ; Biological Techniques.
    In: Springer Nature eBook
    Description / Table of Contents: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
    Type of Medium: Online Resource
    Pages: XIV, 360 p. 94 illus., 85 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071634011
    Series Statement: Methods in Molecular Biology, 2707
    Language: English
    Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.
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  • 9
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Pharmacology. ; Chemistry Data processing. ; Bioinformatics. ; Pharmacology. ; Computational Chemistry. ; Bioinformatics.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition provides new and updated methods and techniques for identification of drug target, binding sites prediction, high- throughput virtual screening, lead discovery and optimization, conformational sampling, prediction of pharmacokinetic properties using computer-based methodologies. Chapters also focus on the application of the latest artificial intelligence technologies for computer aided drug discovery. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computational Drug Discovery and Design, Second Edition aims to effectively utilize computational methodologies in discovery and design of novel drugs.
    Type of Medium: Online Resource
    Pages: XI, 356 p. 1 illus. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071634417
    Series Statement: Methods in Molecular Biology, 2714
    Language: English
    Note: Computer-Aided Drug Discovery and Design – Recent Advances and Future Prospects -- Virtual Screening Process - A Guide in Modern Drug Designing -- Molecular dynamics as a tool for virtual ligand screening -- Antiviral Drug Target Identification and Ligand Discovery -- GRAMM webserver for protein docking -- Protein–ligand blind docking using CB-Dock2 -- Applications of Molecular Dynamics Simulations in Drug Discovery -- Molecular dynamics simulation-based prediction of glycosaminoglycan interactions with drug molecules -- Mining chemogenomic spaces for prediction of drug-target interactions -- Expanding the landscape of amyloid sequences with CARs-DB: a database of polar amyloidogenic peptides from disordered proteins -- Accelerating molecular dynamics simulations for drug discovery -- Exploring the Role of Chemoinformatics in Accelerating Drug Discovery: A Computational Approach. -Recent Deep-Learning Applications to Structure-Based Drug Design -- Techniques for Developing Reliable Machine Learning Classifiers Applied to Understanding and Predicting Protein:Protein Interaction Hot Spots -- AI driven enhancements in drug screening and optimisation -- Applications of big data and AI-driven technologies in CADD (computer-aided drug design) -- Artificial Intelligence in ADME Property Prediction -- Accelerating the discovery and design of antimicrobial peptides with artificial intelligence.
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  • 10
    Subject(s): Immunity. ; Hypersensitivity ; Immune System physiology ; Immunologic Deficiency Syndromes.
    Type of Medium: Book
    Pages: v, 345 pages : , illustrations (chiefly color) ; , 24 cm
    Edition: 7th edition.
    ISBN: 9780443105197
    Language: English
    Location/Call number: Library / QR181:099(7)
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  • 11
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cytology. ; Immunology. ; Bacteria. ; Cell Biology. ; Immunology. ; Bacteria.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field.
    Type of Medium: Online Resource
    Pages: XVI, 576 p. 128 illus., 120 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634370
    Series Statement: Methods in Molecular Biology, 2713
    Language: English
    Note: Macrophage Development and Function -- Fate Mapping Macrophages: From Ontogeny to Functions -- Studying Autophagy in Microglia: Overcoming the Obstacles -- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq -- Isolation of Tissue Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages -- Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue -- Isolation and Flow Cytometry Analysis of Macrophages from the Dermis -- Isolation and Flow Cytometry Analysis of Macrophages from the Kidney -- Isolation and Flow Cytometry Analysis of Intestinal Macrophages -- Isolation and Characterization of Testis Macrophages Using Flow Cytometry -- Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy -- Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages -- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach -- Spectral Flow Cytometry Analysis of Resident Tissue Macrophages -- Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging -- 3D Imaging of Macrophages in Complete Organs -- Whole-Mount Imaging of Adipose Tissue Macrophages -- Functional In Vivo Imaging of Macrophages -- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy -- Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection -- Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH -- Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA) -- Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation -- Detection of G-Quadruplex DNA Structures in Macrophages -- Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models -- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis -- Automated Cell Counting of Macrophages In Situ -- Morphometric Analyses of Macrophages -- Combined Analysis of mRNA Expression and Open Chromatin in Microglia.
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  • 12
    Type of Medium: Unknown
    Pages: xlvi, 2775 p.
    Edition: 7., völlig neu bearbeitete und erweiterte Auflage
    ISBN: 9783452301994
    Series Statement: Heymanns gewerblicher Rechtsschutz
    Language: English
    Location/Call number: M280 / M280:011
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  • 13
    Subject(s): Agriculture. ; Quantitative research. ; Biology Technique. ; Genomics. ; Molecular biology. ; Plant genetics. ; Botanical chemistry. ; Agriculture. ; Data Analysis and Big Data. ; Genomic Analysis. ; Molecular Biology. ; Plant Genetics. ; Plant Biochemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This book addresses complex problems associated with crop improvement programs, using a wide range of programming solutions, for genomics data handling and sustainable agriculture. It describes important concepts in genomics data analysis and sequence-based mapping approaches along with references. The book contains 16 chapters on recent developments in several methods of genomic data analysis for crop improvements and sustainable agriculture, all authored by eminent researchers who are experts in their fields. These chapters focus on applications of a wide range of key bioinformatics topics, including assembly, annotation, and visualization of next-generation sequencing (NGS) data; expression profiles of coding and noncoding RNA; statistical and quantitative genetics; trait-based association analysis, quantitative trait loci (QTL) mapping, and artificial intelligence in genomic studies. Real examples and case studies in the book will come in handy when applying the techniques. The relative scarcity of reference materials covering bioinformatics applications as compared with the readily available books also enhances the utility of this book. The targeted readers of the book are scientists, researchers, and bioinformaticians from genomics and advanced breeding in different areas. The book will appeal to the applied researchers engaged in crop improvements and sustainable agriculture by using bioinformatics tools, students, research project leaders, and practitioners from the various marginal disciplines and interdisciplinary research.
    Type of Medium: Online Resource
    Pages: XI, 374 p. 1 illus. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9789819969135
    Series Statement: Springer Protocols Handbooks,
    Language: English
    Note: Chapter 1. Statistical and Biological Data Analysis using Programming Languages -- Chapter 2. Python for Biologists -- Chapter 3. Assembly, Annotation and Visualization of NGS Data -- Chapter 4. Statistical and Quantitative Genetics Studies -- Chapter 5. Mapping of Quantitative Traits Loci: Harnessing Genomics Revolution for Dissecting Complex Traits -- Chapter 6. Trait Based Association Mapping in Plants -- Chapter 7. META-Analysis of Mapping Studies: Integrating QTLs Towards Candidate Gene Discovery -- Chapter 8. Role of Databases and Bioinformatics Tools in Crop Improvement -- Chapter 9. Overview of the Bioinformatics Databases and Tools for Genome Research and Crop Improvement -- Chapter 10. Public Domain Databases - A Gold Mine for Identification and Genome Reconstruction of Plant Viruses and Viroids -- Chapter 11. Tree Genome Databases: A New Era in the Development of Cyber-infrastructures for Forest Trees -- Chapter 12. Development of Biological Databases for Genomic Research -- Chapter 13. Artificial Intelligence in Genomic Studies -- Chapter 14. Basics of the Molecular Biology: From Genes to its Function.
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  • 14
    Subject(s): Microbiology. ; Pathology. ; Microbiology. ; Pathology.
    In: Springer Nature eBook
    Description / Table of Contents: This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Host-Pathogen Interactions: Methods and Protocols, Second Edition contributes to the study of host-pathogen interactions with numerous techniques that can be used in a variety of bacteria.
    Type of Medium: Online Resource
    Pages: XIV, 270 p. 58 illus., 48 illus. in color. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071636176
    Series Statement: Methods in Molecular Biology, 2751
    Language: English
    Note: Host-Pathogen Interaction: Biology and Public Health -- Genetic Association Studies in Host-Pathogen Interaction Analysis -- Live-Cell Fluorescence Imaging for Virus/Host Interactions -- Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea -- Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors -- Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem -- Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae -- A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent -- Quantification of Mixed-Linkage β-Glucan (MLG) in Bacteria -- Surface Plasmon Resonance as a Tool to Elucidate the Molecular Determinants of Key Transcriptional Regulators Controlling Rhizobial Lifestyles -- Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures -- A Workflow for the Functional Characterization of Noncoding RNAs in Legume Symbiotic Bacteria -- Methods for Studying Swimming and Surface Motilities in Rhizobia -- Isolation, Quantification, and Visualization of Extracellular Membrane Vesicles in Rhizobia Under Free-Living Conditions -- Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids -- Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes -- A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids -- New Inoculation Strategy for Legume Based on Rhizobium-Metabolite Co-Encapsulation.
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  • 15
    Subject(s): Cancer. ; Cancer Animal models. ; Cancer Biology. ; Cancer Models.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also included, as well as imaging techniques that allow for the visualization of cancer at the cellular and tissue level. The book closes with a detailed description of necropsy, which is essential for obtaining good biomaterial for translational research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Models of Cancer: Methods and Protocols serves as an ideal guide to these controlled and reproducible model experimental systems to study different aspects of cancer biology, including prevention, development, progression, and treatment.
    Type of Medium: Online Resource
    Pages: X, 198 p. 74 illus., 71 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071637142
    Series Statement: Methods in Molecular Biology, 2773
    Language: English
    Note: In Vivo Modeling of Leukemia by Murine Stem Cell Transplantation -- NOTCH1-Induced T-Cell Acute Lymphoblastic Leukemia In Vivo Models -- Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine -- Establishment of Mouse Orthotopic Urinary Bladder Tumor Model and Its Analysis by Light and Electron Microscopy -- Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) Model of Colorectal Cancer -- Isolating Primary Tumor Cells from the MMTV-PyMT Mouse Model and Their Use in Developing an Orthotopic Mouse Model of Breast Cancer -- Transplantable Subcutaneous Tumor Models -- Preclinical Mouse Metastatic Model Established through Induced Lung Metastases -- In Vivo Wound Healing Model for Characterization of Gene Electrotransfer Effects in Mouse Skin -- Partial-Volume Irradiation of Murine Tumors -- In Vivo Bioluminescence and Fluorescence Imaging: Optical Tool for Cancer Research -- Cancer Imaging by Intravital Microscopy: The Dorsal Window Chamber Model -- Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber -- Mouse Melanoma Model in Tumor Vaccines and Immunotherapy Research -- Immunospot Assessment of T Cell Responses in Preclinical Tumor Models with Undefined Target Antigens -- Necropsy in Mouse Research.
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  • 16
    Subject(s): Food science. ; Food Science.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details state-of-the art instrumental and sensory wine testing procedures for a broad range of wine applications, focusing on instrumental, sensory, gas chromatography-olfactometry. Chapters detail introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Wine Analysis and Testing Techniques aims to ensure successful results in the further study of this vital field.
    Type of Medium: Online Resource
    Pages: XIII, 248 p. 59 illus., 45 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636503
    Series Statement: Methods and Protocols in Food Science,
    Language: English
    Note: DNA based methods for wine traceability and varietal authentication using Single Nucleotide Polymorphism genotyping assays -- Optimization of microbial DNA extraction from wine, juice, and sap for community-based genome studies -- Fluorescence spectroscopy for red wine authentication -- Mass spectrometry based methods for the characterization of wine flavonoids -- Quantification of Proteins in white and rosé Wines. -White and rosé Wine Haze Risk (WHR) estimation -- Determination of biogenic amines in wines -- Determination of free and glycosidically-bound fractions responsible of grape musts aroma by solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) -- WINE VOLATILOMICS -- In-mouth wine aroma analysis -- Wine descriptive sensory profiling -- Rapid and cost-effective methods for wine sensory profiling: napping and sorting -- Rapid and cost-effective methods for wine profiling: CATA/RATA -- Time-Intensity methodology for wine flavour evaluation -- Temporal Dominance of Sensations (TDS) applied to wine sensory evaluation -- Evaluation of hedonic and emotional response evoked by wines -- Analysis of wine impact odorants by gas chromatography-olfactometry.
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  • 17
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunologic diseases. ; Therapeutics. ; Immunological Disorders. ; Therapeutics.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols, Second Edition aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study. .
    Type of Medium: Online Resource
    Pages: XI, 354 p. 83 illus., 52 illus. in color. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071636824
    Series Statement: Methods in Molecular Biology, 2766
    Language: English
    Note: Collagen-induced arthritis models -- Human xenograft model -- Scaffolded chondrogenic spheroid-engrafted model -- Denervation-induced sarcopenia model -- Long-term constant subcutaneous drug administration -- Clinical Scoring of Disease Activity in Animal Models -- Histological analyses of arthritic joints in collagen-induced arthritis model mice -- Preparation of Joint Extracts -- Production of immunizing antigen proteoliposome using cell-free protein synthesis system -- Re-construction of protein/liposome complex -- Production of neutralizing antibody -- Autoantibody profiling using human autoantigen protein array and AlphaScreen -- Generation of Specific Aptamers -- Detailed Protocol for Predicting 3D Structure of DNA Aptamers and Performing In Silico Docking Calculations -- RNA interference ex vivo -- Lentiviral-mediated systemic RNA interference in vivo -- Lentiviral production platform -- Mesenchymal stem cell engineering -- Screening of Ca2+ influx in lymphocytes -- Single-cell Ca2+ imaging -- Electrophysiological methods to measure Ca2+ current -- Evaluation of mitochondrial respiratory function in murine splenocytes -- The Functional Assessment of T Cells -- Release of antibodies and cytokines from B cells -- Evaluation of autoreactive responses -- Macrophage polarization and osteoclast differentiation -- Scanning electron microscopic analysis of the bone-resorption activity in mature osteoclasts -- Animal Models of Vasculitis -- Evaluation of skin damage under UV exposure -- Bulk RNA-seq assessment of murine spleen using a portable MinION sequencing device -- Institutional review board considerations for clinical trials -- Design an intervention study -- Assessment of disease activity, structural damage, and function in rheumatoid arthritis -- Assessment of musculoskeletal ultrasound of rheumatoid arthritis -- 16S rRNA gene amplicon analysis of human gut microbiota.
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  • 18
    Subject(s): Botany. ; Plant Science.
    In: Springer Nature eBook
    Description / Table of Contents: This volume presents vital techniques for the commercial micropropagation of plants. It explores numerous micropropagation protocols in different types of temporary immersion systems for plant species with agricultural, medicinal, ornamental, and forestry interest, detailing the type of bioreactor and the optimal parameters necessary to guarantee obtaining the largest number of commercial propagules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Micropropagation Methods in Temporary Immersion Systems serves as an ideal guide to allow for the semi-automation and reduction of production costs during the micropropagation of plants.
    Type of Medium: Online Resource
    Pages: XIII, 252 p. 68 illus., 65 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636541
    Series Statement: Methods in Molecular Biology, 2759
    Language: English
    Note: Temporary Immersion Systems in Plant Micropropagation -- Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation -- Use of Temporary Immersion Systems in the Establishment of Biofactories -- Large-Scale Micropropagation of Vanilla (Vanilla planifolia Jacks.) in a Temporary Immersion Bioreactor (TIB) -- Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting -- Micropropagation of Stevia (Stevia rebaudiana Bert.) in RITA® -- In Vitro Multiplication of Agave (A. marmorata and A. potatorum) by Temporary Immersion in SETIS™ Bioreactor -- BioMINT: A Temporary Immersion System for Agave Micropropagation -- Plant Regeneration of Agave cupreata by Somatic Embryogenesis in a Temporary Immersion System with Silver Nanoparticles -- Micropropagation of Chayote (Sechium edule L.) var. virens levis in RITA® -- Direct Shoot from Root and True-to-Type Micropropagation of Limonium ‘Misty Blue’ in Partially Immersed Culture on an Aluminum Mesh Raft -- Increased Multiplication Rates of Vriesea hieroglyfica (Carriere) E. Morren through a Temporary Immersion System -- Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor -- Micropropagation of Guarianthe skinneri (Bateman) Dressler & W. E. Higging in Temporary Immersion Bioreactors -- Alstroemeria Micropropagation in a RITA® Temporary Immersion System -- Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems -- In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems -- Multiplication of Cupressus guadalupensis Using the RITA® Temporary Immersion System -- Secondary Embryogenesis of Linaloe in Temporary Immersion Bioreactors Type RITAÒ -- Temporary Immersion System for Biomass Production of Salvia spp: A Mini-Review -- Orchid Micropropagation Using Temporary Immersion Systems: A Review -- Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems.
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  • 19
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Developmental biology. ; Toxicology. ; Embryology. ; Developmental Biology and Stem Cells. ; Medical Toxicology. ; Embryology.
    In: Springer Nature eBook
    Description / Table of Contents: This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
    Type of Medium: Online Resource
    Pages: XVI, 627 p. 113 illus., 96 illus. in color. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071636251
    Series Statement: Methods in Molecular Biology, 2753
    Language: English
    Note: Updating an Overview of Teratology -- Guidelines on Development Toxicity Tests: Brief Insights -- Development Features on the Selection of Animal Models for Teratogenic Testing -- Virus as Teratogenic Agents -- The Future of the Teratogenicity Testing -- The First Steps on AOPs' Concepts, Development, and Applications in Teratology -- Machine Learning to Predict Teratogenicity: Theory and Practice -- Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity -- Developmental Toxicity Using the Rat Whole Embryo Culture -- A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs -- Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry -- Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo -- Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds -- Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects -- Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure -- Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster -- Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb -- Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb -- Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX -- Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing -- Angiogenesis Assay for Live and Fixed Zebrafish Larvae -- High-Resolution Respirometry for the Assessment of Teratogenic Chemicals -- A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae -- Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos -- Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish -- Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response -- Alcian Blue Staining for Chondrocranium Development in Zebrafish -- Protocol of Geometric Morphometrics for Teratogenicity Testing -- Biochemical Markers for Liver Injury in Zebrafish Larvae -- Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae -- Metabolomic Fingerprint Assay in Zebrafish Embryos -- Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae -- A Chromogenic Quantification of Protein Expression in Zebrafish Larvae -- Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model -- Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos -- Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos -- Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos -- Cell-Free DNA and Next-Generation Sequencing for Prenatal Diagnosis.
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  • 20
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): RNA Metabolism. ; Genetics. ; Virology. ; RNA Metabolism. ; Genetics and Genomics. ; Virology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA.
    Type of Medium: Online Resource
    Pages: XI, 142 p. 31 illus., 20 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071637029
    Series Statement: Methods in Molecular Biology, 2771
    Language: English
    Note: Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation -- Detection of dsRNA by Acridine Orange Staining -- Isolation of dsRNA from Plants by Cellulose Chromatography -- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column -- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay -- Detection of dsRNA with the Fluorescence In Situ Hybridization (FISH) Assay -- Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana -- Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription -- Inducible Expression of dsRNA in Escherichia coli -- In Vivo Production of dsRNA Using Bacteriophage ϕ6 in Pseudomonas syringae Cit7 Cells -- Bacteria-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management -- Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System -- Co-Immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana -- Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana -- Identification of Mycoviruses by dsRNA Extraction -- Production of Double-Stranded RNA In Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing -- Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea -- Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus.
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  • 21
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cancer. ; Cancer Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hypoxia: Methods and Protocols is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies. .
    Type of Medium: Online Resource
    Pages: XI, 266 p. 69 illus., 59 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636336
    Series Statement: Methods in Molecular Biology, 2755
    Language: English
    Note: Genetically Encoded Reporters to Monitor Hypoxia -- Detection of Hypoxia in 2D and 3D Cell Culture Systems using Genetically Encoded Fluorescent Hypoxia Sensors -- Mapping the Fate of Hypoxic Cells using an Irreversible Fluorescent Switch -- Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xenografted Tumor Imaging in Nude Mice -- A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro -- Oxygen Assessment in Tumors In Vivo using Phosphorescence Lifetime Imaging Microscopy -- Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo -- Measuring Pericellular Oxygen Tension for In Vitro Cell Culture -- A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography -- Photoacoustic Lifetime Imaging of Hypoxia -- Staining Hypoxic Areas in Frozen and FFPE Tissue Sections with Hypoxyprobe™ -- Multiplex Immunofluorescence Staining Protocol for Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples -- Detecting Hypoxia-Inducible Factor Levels and Activity -- Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells -- Measurement of Metabolic Alteration in Immune Cells under Hypoxia -- Evaluation of Oxygen Consumption Rates In Situ -- Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2-D and 3-D Tumor Models -- Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells.
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  • 22
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Proteins. ; Proteins.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins.
    Type of Medium: Online Resource
    Pages: XIV, 355 p. 89 illus., 77 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636664
    Series Statement: Methods in Molecular Biology, 2762
    Language: English
    Note: Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells -- Production and Purification of Filovirus Glycoproteins -- Modification of N-Linked Glycan Sites in Viral Glycoproteins -- Production of Influenza Virus Glycoproteins Using Insect Cells -- SARS-CoV-2 S-Protein – Ace2 Binding Analysis Using Surface Plasmon Resonance -- A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain -- Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis -- Analysis of Caenorhabditis Protein Glycosylation -- Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates -- Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors -- Production and Purification of Antibodies in Chinese Hamster Ovary Cells -- Mammalian Antigen Display for Pandemic Countermeasures -- Analysis of Native and Permethylated N-Glycan Isomers Using MGC-LC-MS Techniques -- Targeted Glycoproteomics Analysis Using MRM/PRM Approaches -- Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches -- Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A -- O-Glycoproteomics Sample Preparation and Analysis Using NanoHPLC and Tandem MS -- Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers -- Cell-Free Systems for the Production of Glycoproteins -- Considerations for Glycoprotein Production.
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  • 23
    Subject(s): Cancer. ; Liver. ; Physiology. ; Cancer Biology. ; Hepatic Physiology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction of ectopic implantation. The second half of this book covers a diverse array of techniques applied to characterize the biochemical and cellular composition of hepatic malignancies that operate at the single-cell and histological levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Liver Carcinogenesis: Methods and Protocols, is a valuable resource for students and scientists who are interested in driving progress in the important field of liver cancer research. .
    Type of Medium: Online Resource
    Pages: XII, 228 p. 44 illus., 42 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636947
    Series Statement: Methods in Molecular Biology, 2769
    Language: English
    Note: Orthotopic Model of Hepatocellular Carcinoma in Mice -- Diethylnitrosamine Induction of Hepatocarcinogenesis in Mice -- Diethylnitrosamine Induced Liver Tumorigenesis in Mice Under High-Hat High-Sucrose Diet: Stepwise High-Resolution Ultrasound Imaging and Histopathological Correlations -- A Mouse Model of Non-Alcoholic Steatohepatitis and Hepatocellular Carcinoma Induced by Western Diet and Carbon Tetrachloride -- A Mouse Model of Hepatocellular Carcinoma Induced by Streptozotocin and High Fat Diet -- Hydrodynamic Transfection of Hepatocytes for the Study of Hepatocellular Carcinogenesis -- Experimental Model of Biliary Tract Cancers: Subcutaneous Xenograft of Human Cell Lines in Immunodeficient Nude Mouse -- Oncogene-Driven Induction of Orthotopic Cholangiocarcinoma in Mice -- Isolation of Primary Mouse Hepatocytes and Non-Parenchymal Cells from a Liver with Precancerous Lesions -- Flow Cytometry Assessment of Lymphocyte Populations Infiltrating Liver Tumors -- Immunofluorescent Staining of Human Hepatic Multicellular Spheroids: A Model for Studying Liver Diseases -- Single-Cell Characterization of the Tumor Ecosystem in Liver Cancer -- Chromatin and DNA Dynamics in Mouse Models of Liver Cancer -- Targeted Analysis of Glycerophospholipids and Mono-, Di- or Tri-Acylglycerides in Liver Cancer -- Biomarker Identification in Liver Cancers using Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Imaging: An Approach for Spatially Resolved Metabolomics -- Kinetic Modeling of Hepatic Metabolism and Simulation of Treatment Effects.
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  • 24
    Subject(s): Proteins. ; Therapeutics. ; Biomedical engineering. ; Proteins. ; Therapeutics. ; Biomedical Engineering and Bioengineering.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins. .
    Type of Medium: Online Resource
    Pages: XI, 248 p. 62 illus., 47 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634691
    Series Statement: Methods in Molecular Biology, 2720
    Language: English
    Note: Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production -- Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains -- Selective and Site-Specific Incorporation of Non-Standard Amino Acids within Proteins for Therapeutic Applications -- An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions -- Split-Protein Therapeutic Platforms: Identifying Binder Pairs -- Metal-Mediated Ligand Affinity Chemistry (MLAC) -- Recombinant Elastin‐Based Bioelastomers for Biomedical Applications -- Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery -- Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines -- Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs -- Unnatrual Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics -- Formulation of Chitosan-Zein Nano-in-Microparticles for Oral DNA Delivery -- Engineering E2 Bionanoparticles for Targeted Delivery of Chemotherapeutics to Breast Cancer Cells -- Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes -- Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines -- Melt-Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants.
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  • 25
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Food science. ; Microbiology. ; Food Science. ; Microbiology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details preparations, separations, identification, analysis of postbiotics types and mechanism, uses of postbiotics in health, pharma, aquacultures, and the food industry. Divided into five sections chapters provide methods on antimicrobials, antibiofilm, anti-inflammatory, antiallergies, antiobesity, meurotransmitter activity, dietary supplementation and Immunomodulatory activity of postbiotics, postbiotics as biopreservatives , and as well as food packing material. . Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Postbiotics aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
    Type of Medium: Online Resource
    Pages: XXI, 424 p. 68 illus., 50 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634219
    Series Statement: Methods and Protocols in Food Science,
    Language: English
    Note: Isolation and Identification of Probiotics Microorganisms -- Isolation and Identification of Lactic Acid Bacteria -- Isolation and Identification of Bifidobacterium Spp. -- Isolation and Identification Of Yeasts -- Mass Propagation of Lactic Acid Bacteria -- Preparation of Postbiotics From Bifidobacterium Sp. -- Preparation of Postbiotics From Lactococcus Spp. -- Preparation of Postbiotics From Saccharomyces Spp. -- Preparation of Postbiotics From Streptomyces Sp. -- Preparation of Postbiotics From Streptococcus Sp. -- Preparation of Postbiotics from Bacillus -- Chemical Characterization and Identification of Postbiotics From Probiotic Microbes -- Thermal Methods of Postbiotics Preparation -- Non-Thermal Preparation of Postbiotics -- Next-Generation Sequence Analysis of Postbiotics In Fermented Dairy Food -- Next Generation Sequence Analysis of Postbiotics In Fermented non- Dairy Food -- Harvesting of Postbiotics And Its Assessment -- Analysis and Spectral Characterization of Exopolysaccharide Postbiotics by FT-IR and NMR -- Analysis and Identification Short Chain Fatty Acid Postbiotics By Gas Chromatography -- Analysis and identification of Biosurfactants Postbiotics -- Analysis and Identification Peptide Postbiotics By LC-MS, MOLTI-TOF Mass Spectrometry -- Analysis and Identification of Postbiotic Enzymes -- UHPLC And MS/MS Mediated Analysis And Quantification Of Postbiotic Cobalamin (Vitamin B12) From Propionibacterium Freudenreichii -- Separation and Identification Of Neurotransmitters From Of Postbiotics -- Immuno Modulatory Activity of Postbiotics From Lactobacillus -- Antibacterial Activity of Postbiotics -- Antifungal Activity of Postbiotics -- Antiviral Activity of Postbiotics -- Antiprotozoan Activity Of Postbiotics -- Anthelminthic Activity Of Postbiotics -- Antibiofilm Activity Of Postbiotics -- Evaluation Of Antioxidant Activity Of Postbiotics In Cell Cultures -- Anti-Allergies Activity Of Postbiotics -- Anti-inflammatory Activity of Postbiotics -- Anti-obesity activity of postbiotics -- Angiotensin 1-converting enzyme inhibitory postbiotics from fermented soybean -- Bacteriocin Postbiotics for tuberculosis drug development -- Postbiotics For Typhoid Drug Development -- Immunomodulatory Activity Of Postbiotics In Goat -- Immunomodulatory Activity of Postbiotics in Chicks -- Immunomodulatory Activity Of Postbiotics In Pigs -- Invivo Immunomodulatory Study of Postbiotics in Mice -- Dietary Supplementation and Immunomodulatory Activity Of Postbiotics In Fish -- Dietary Supplementation and Immunomodulatory Activity of Postbiotics in Shrimp -- Dietary Supplementation and Immunomodulatory activity of Postbiotics in Oyster -- Dietary supplementation and Immunomodulatory activity of Crab -- Bio preservation of meat and fish products using Postbiotics -- Biopreservation of Dairy Products through Postbiotics -- Biopresrvation Of Fruits And Vegetables Using Postbiotics -- Biopreservation of food using bacteriocin -- Postbiotics Food Packing Based on Organic acids -- Postbiotics Food Packaging using Peptides -- Postbiotics Food Packing using Bacteriocin -- Degradation of Chlorpyrifos pesticide using a postbiotic enzyme of Lactic acid bacteria -- Dosa Postbiotics Food Preparations -- Preservation of Postbiotics by Spray-Drying Microencapsulation .
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  • 26
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Medicine Research. ; Biology Research. ; Internal medicine. ; Biomedical Research. ; Internal Medicine.
    In: Springer Nature eBook
    Description / Table of Contents: This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs. .
    Type of Medium: Online Resource
    Pages: X, 264 p. 69 illus., 54 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634295
    Series Statement: Methods in Molecular Biology, 2711
    Language: English
    Note: Determination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro -- Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice -- Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux -- Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy -- Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System -- Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model -- Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier -- Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy -- Studying Angiogenesis using Matrigel In Vitro and In Vivo -- Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy -- Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells -- Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs) -- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification -- Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay -- Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model -- Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells -- An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies -- Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro -- Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression -- Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer -- Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy.
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  • 27
    Subject(s): Bacteria. ; Microbiology. ; Bacteria. ; Microbiology.
    In: Springer Nature eBook
    Description / Table of Contents: This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
    Type of Medium: Online Resource
    Pages: X, 246 p. 51 illus., 40 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634738
    Series Statement: Methods in Molecular Biology, 2721
    Language: English
    Note: CRISPR/Cas9-based genome editing of Pseudomonas aeruginosa -- Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis using Mobile-CRISPRi -- Engineering green-light-responsive heterologous gene expression in Pseudomonas -- Fluorescence-based evaluation of cyclic di-GMP levels in Pseudomonas aeruginosa -- Whole-cell biosensors for qualitative and quantitative analysis of quorum sensing signal molecules and the investigation of quorum quenching agents -- A Pseudomonas aeruginosa-suitable fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs -- The Pseudomonas aeruginosa resistome: permanent and transient antibiotic resistance, an overview -- Biosensors for inducers of transient antibiotic resistance -- Pseudomonas aeruginosa soluble pyocins as antibacterial weapons -- Assays for studying Pseudomonas aeruginosa secreted proteases -- Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms -- Broad genome sequencing of environmental and clinical strains and genotyping -- Genome-scale analysis of the structure and function of RNA pathways and networks in Pseudomonas aeruginosa -- In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. Improving the predictive value of preclinical mouse models of Pseudomonas aeruginosa respiratory infection to evaluate antibiotic efficacy -- Emerging in vitro models for the study of infection and pathogenesis of Pseudomonas aeruginosa and testing of antibacterial agents.
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  • 28
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Biomedical engineering. ; Genetics. ; Bioinformatics. ; Biomedical Engineering and Bioengineering. ; Genetics. ; Bioinformatics.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters guide readers through culprit genes, in-silico drug discovery techniques, genome-wide ChIP-X data, high-Throughput Transcriptomic Data Exome Sequencing, Next-Generation Sequencing, Fuorescence Spectroscopy, data analysis in Bioinformatics, Computational Biology, and S-system based modeling of GRN. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Engineering of Regulatory Networks aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
    Type of Medium: Online Resource
    Pages: X, 327 p. 72 illus., 64 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634615
    Series Statement: Methods in Molecular Biology, 2719
    Language: English
    Note: Molecular Modeling Techniques and in-Silico Drug Discovery -- Systems Biology Approach to Analyse Microarray Datasets for Identification of Disease-Causing Genes: Case Study of Oral Squamous cell Carcinoma -- Fluorescence Spectroscopy: A Useful Method to Explore the Interactions of Small Molecule Ligands with DNA Structures -- Inference of Dynamic Growth Regulatory Network in Cancer Using high-Throughput Transcriptomic Data -- Implementation of Exome Sequencing to Identify Rare Genetic Diseases -- Emerging Trends in Big Data Analysis in Computational Biology and Bioinformatics in Health Informatics: A Case Study on Epilepsy and Seizures -- New Insights into Clinical Management for Sickle-Cell Disease: Uncovering the Significance Pathways Affected By the Involvement of Sickle Cell Disease -- A Review on Computational Approach for S-system Based Modeling of Gene Regulatory Network -- Big Data in Bioinformatics and Computational Biology: Basic Insights -- Identification of Culprit Genes for Different Diseases by Analysing Microarray Data -- Big Data Analysis in Computational Biology and Bioinformatics -- Prediction and Analysis of Transcription Factor Binding Sites to Understand Gene Regulation: Practical Examples and Case Studies using R Programming -- Hubs and Bottlenecks in Protein-Protein Interaction Networks -- Next-Generation Sequencing to Study the DNA Interaction Nac Deep Learning for Predicting Gene Regulatory Networks: A Step-by-Step Protocol in R -- Deep Learning for Predicting Gene Regulatory Networks: A Step-by-Step Protocol in R -- Computational inference of Gene Regulatory Network using genome-wide ChIP-X data -- Reverse Engineering in Biotechnology: The Role of Genetic Engineering in Synthetic Biology.
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  • 29
    Subject(s): Biophysics. ; Biotechnology. ; Biophysics. ; Biotechnology.
    In: Springer Nature eBook
    Description / Table of Contents: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. .
    Type of Medium: Online Resource
    Pages: XIV, 511 p. 130 illus., 109 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071633779
    Series Statement: Methods in Molecular Biology, 2694
    Language: English
    Note: Introduction to Optical Tweezers: Background, System Designs, and Applications -- Quantifying ATP-Independent Nucleosome Chaperone Activity with Single Molecule Methods -- Protein Tethering for Single-Molecule Force Spectroscopy -- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies -- Probing Mitotic Chromosome Mechanics Using Optical Tweezers -- A Brief Introduction to Single-Molecule Fluorescence Methods -- Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans -- Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays -- Fluorescence Microscopy of Nanochannel-Confined DNA -- Single-Molecule FRET X -- Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Sites of Nucleoprotein Complexes -- Measuring Transcription Dynamics of Individual Genes Inside Living Cells -- Single-Molecule FRET Resolved Protein Dynamics from Plasmid to Data in Six Steps -- Atomic Force Microscopy: An Introduction -- Atomic Force Microscopy of Viruses: Stability, Disassembly, and Genome Release -- Unfolding and Refolding Proteins using Single-Molecule AFM -- Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy -- An Introduction to Magnetic Tweezers -- Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay -- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions -- Detecting DNA Loops Using Tethered Particle Motion -- Single-Cell Measurements using Acoustic Force Spectroscopy (AFS) -- DNA Origami-Based Single-Molecule Force Spectroscopy and Applications.
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  • 30
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Bacteria. ; Cytology. ; Bacteria. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource.
    Type of Medium: Online Resource
    Pages: XI, 240 p. 39 illus., 31 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634912
    Series Statement: Methods in Molecular Biology, 2727
    Language: English
    Note: Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall -- Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography -- Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy -- Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi -- Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis -- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum -- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids -- Type I Lipoteichoic Acid (LTA) Detection by Western Blot -- Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy -- Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris -- Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography -- Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy -- Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase -- Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins -- Tracking Cell Wall Anchored Proteins in Gram-Positive Bacteria -- Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy -- Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers -- Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy.
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  • 31
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Genetic transcription. ; Biology Technique. ; Gene expression. ; Gene Transcription. ; Gene Expression Analysis.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deadenylation: Methods and Protocols aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression.
    Type of Medium: Online Resource
    Pages: XI, 326 p. 58 illus., 47 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634813
    Series Statement: Methods in Molecular Biology, 2723
    Language: English
    Note: The reconstitution of human CCR4-NOT from purified proteins and an assay of its deadenylation activity -- In vitro reconstitution of the Drosophila melanogaster CCR4-NOT complex to assay deadenylation -- Analysis of human mRNA deadenylation complexes via high-resolution gel electrophoresis -- Quantitative biochemical analysis of deadenylase enzymes using fluorescence and chemiluminescence-based assays -- A FRET-based assay to quantify enzymatic rates and explore the mechanisms of RNA deadenylases in heterogeneous environments -- Measuring Poly-Adenosine Tail Length of RNAs by High Resolution Northern Blotting Coupled with RNase H Cleavage -- An RNA-ligation-based RACE-PAT assay to monitor poly(A) tail length of mRNAs of interest -- Dissecting the role of the Ccr4-Not deadenylase complex in pluripotency and differentiation -- Measuring proximity-mediated function of mRNA regulatory proteins by engineered tethering.-Tethered mRNA Amplifier: A Novel Approach to Increase Protein Expression -- RNA binding protein-mediated mRNA deadenylation in mammalian cell extracts -- Transcriptome-wide analysis of mRNA adenylation status in yeast using nanopore sequencing -- Sequencing of transcriptome-wide poly(A) tails by PAIso-seq -- Nano3′RACE: a method to analyze poly(A) tail length and nucleotide additions at the 3′ extremity of selected mRNAs using nanopore sequencing -- Quantification of poly(A) tail length and terminal modifications using direct RNA sequencing -- Differential poly(A) tail length analysis using nanopore sequencing -- Single-molecule poly(A) tail sequencing (SM-PATseq) using the PacBio platform -- Mathematical models for the poly(A) tail shortening process.
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  • 32
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cell organelles. ; Cytology. ; Organelles. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. .
    Type of Medium: Online Resource
    Pages: XII, 278 p. 56 illus., 50 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071635070
    Series Statement: Methods in Molecular Biology, 2725
    Language: English
    Note: Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium -- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures -- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells -- Expansion Microscopy of Ciliary Proteins -- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes -- Structural Analysis of Sperm Centrioles Using N-STORM -- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium -- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets -- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging -- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells -- Proximity Mapping of Ciliary Proteins by BioID -- Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags -- Primary Human Nasal Epithelial Cell Culture -- BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation -- High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models -- Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods -- Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.
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  • 33
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Food Microbiology. ; Food science. ; Food Sensory evaluation. ; Nutrition   . ; Food Analysis. ; Chemistry. ; Food Microbiology. ; Food Science. ; Sensory Evaluation. ; Nutrition. ; Food Chemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This Open Access book covers the concept of umami, the unique taste imparted by the amino acid glutamate, was first described in 1908 by Dr. Kikunae Ikeda of Tokyo University. Over the past century, hundreds of studies have explored the mechanistic underpinnings of the taste, leading to the characterization of the umami taste receptor in 2002. How this fifth basic taste figures into nutrition and health, however, remains underexplored. Umami: Taste for Health provides an overview of the relationship between umami and human health. Authors explain how glutamate not only produces a characteristic oral sensation in the mouth but also functions as a signaling molecule to induce physiological responses. With the support of recent studies, the book demonstrates how the taste properties of umami make glutamate a promising substance to lower salt intake, promote satiation and support healthier aging. The text also covers practical culinary applications to increase umami flavor and practical usage of umami for promoting healthy eating. Provides an overview of the relationship between umami and human health; Explores the potential of glutamate to lower salt intake, promote satiation and support healthier aging; Covers practical culinary applications of umami flavor and practical usage of umami for promoting healthy eating.
    Type of Medium: Online Resource
    Pages: XIII, 198 p. 33 illus. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9783031326929
    Series Statement: Food and Health,
    Language: English
    Note: 1. Umami and MSG -- 2. Sensory Physiology of Umami -- 3. Umami and Salty: A Cooperative Pair -- 4. Protein, Umami and Satiety -- 5. Development and Umami -- 6. Umami and Healthy Aging -- 7. Umami As A Component of Healthy Diets -- 8. Practicalities from Culinology.
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  • 34
    Subject(s): Developmental biology. ; Regenerative medicine. ; Cytology. ; Developmental Biology and Stem Cells. ; Regenerative Medicine and Tissue Engineering. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Embryo Models In Vitro: Methods and Protocols serves as an ideal guide to these exciting and innovative advances in developmental biology.
    Type of Medium: Online Resource
    Pages: XII, 323 p. 3 illus. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636862
    Series Statement: Methods in Molecular Biology, 2767
    Language: English
    Note: Generation of Human Blastoids from Naïve Pluripotent Stem Cells -- Human Pre-Gastrulation Embryo Culture in 3D Condition -- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System -- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models -- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology -- Endometrial Assembloids to Model Human Embryo Implantation In Vitro -- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE) -- Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells -- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells -- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells -- Generation of Stem Cell-Based Mouse Embryo-Like Structures -- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond -- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts -- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast -- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos -- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas -- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL -- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis -- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence -- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics -- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
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  • 35
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cytology. ; Materials Microscopy. ; Cell Biology. ; Microscopy.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field. .
    Type of Medium: Online Resource
    Pages: XIII, 260 p. 54 illus., 45 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071636213
    Series Statement: Methods in Molecular Biology, 2752
    Language: English
    Note: Single Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinder,- Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ Systems -- Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients -- Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating Cells -- Isolation of Single Circulating Tumor Cells using VyCAP Puncher System -- Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell -- Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients -- Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells -- Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules -- Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNA -- Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells -- Single Cell MicroRNA Sequencing Library Preparation -- Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western Platform -- Imaging of Sub-Cellular Distribution of Platinum in Single Cells using Laser Ablation Inductively Coupled Plasma Mass Spectrometry -- Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression -- Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts.
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  • 36
    Unknown
    Unknown
    Weinheim : Wiley-VCH
    Type of Medium: Unknown
    Pages: 206 p. : , ill
    ISBN: 9783527721375
    Series Statement: Lernen leicht gemacht
    Language: English
    Note: privat : verantwortungsvoller Umgang mit ChatGPT und anderen KI-Tools Anmerkung: Titelzusatz auf Cover: Technische Grundlagen von ChatGPT und OpenAI. ChatGPT gewinnbringend nutzen: beim Lernen, bei der Arbeit und privat. Verantwortungsvoller Umgang mit ChatGPT und anderen KI-Tools.
    Location/Call number: Library / QA76.7: 141
    Location/Call number: M120 / M120:413
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  • 37
    Unknown
    Unknown
    Boca Raton : CRC Press
    Type of Medium: Unknown
    Pages: 442 p.
    Edition: 2nd ed.
    ISBN: 9781032370385
    Language: English
    Location/Call number: Library / QH505:018(2)
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  • 38
    Subject(s): Medical physics Mathematics. ; Monte Carlo method.
    Type of Medium: Online Resource
    Pages: 1 online resource (xiv, 335 pages) : , illustrations.
    ISBN: 9781000987614
    Series Statement: Series in Medical Physics and Biomedical Engineering Series
    Language: English
    Note: Includes index.
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  • 39
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS German Medical Science; VOL: 21; DOC01 /20230303/
    Publication Date: 2023-03-27
    Description: For the purposes of this guideline, a diving accident is defined as an event that is either potentially life-threatening or hazardous to health as a result of a reduction in ambient pressure while diving or in other hyperbaric atmospheres with and without diving equipment. This national consensus-based guideline (development grade S2k) presents the current state of knowledge and recommendations on the diagnosis and treatment of diving accident victims. The treatment of a breath-hold diver as well as children and adolescents does not differ in principle.In this regard only unusual tiredness and itching without visible skin changes are mild symptoms.The key action statements: on-site 100% oxygen first aid treatment, immobilization/no unnecessary movement, fluid administration and telephone consultation with a diving medicine specialist are recommended.Hyperbaric oxygen therapy (HBOT) remains unchanged as the established treatment in severe cases, as there are no therapeutic alternatives. The basic treatment scheme recommended for diving accidents is hyperbaric oxygenation at 280 kPa.
    Description: Ein Tauchunfall im Sinne dieser Leitlinie ist ein potenziell lebensbedrohliches oder gesundheitsschädigendes Ereignis, hervorgerufen durch Abfall des Umgebungsdruckes beim Tauchen oder aus sonstiger hyperbarer Atmosphäre mit und ohne Tauchgerät. Diese nationale S2k-Leitlinie legt den aktuellen Stand der Erkenntnisse und der konsentierten Empfehlungen in der Diagnostik und Behandlung von Patienten nach Tauchunfällen dar. Die Behandlung von Apnoetauchern sowie Kindern und Jugendlichen unterscheidet sich prinzipiell nicht.Milde Symptome sind nur die auffällige Müdigkeit und ein Hautjucken ohne sichtbare Hautveränderungen.Wesentliche Bedeutung bei der Versorgung von Tauchunfällen hat die frühzeitige Atmung von 100%igem Sauerstoff. Weiterhin werden die Ruhiglagerung/keine unnötige Bewegung, eine moderate Flüssigkeitsgabe und eine Taucherärztliche Telefonberatung empfohlen.Die hyperbare Sauerstofftherapie (HBOT) ist bei schweren Dekompressionsunfällen unverändert ohne therapeutische Alternative. Als Behandlungsschema wird grundsätzlich eine HBOT bei 280 kPa empfohlen.
    Subject(s): diving accident ; decompression sickness ; decompression illness ; arterial gas embolism ; oxygen ; hyperbaric oxygen therapy ; Tauchunfall ; Dekompressionserkrankung ; arterielle Gasembolie ; Sauerstoff ; hyperbare Sauerstofftherapie ; ddc: 610
    Language: English
    Type: article
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  • 40
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC07 /20230327/
    Publication Date: 2023-03-27
    Description: The objective is to provide a comprehensive overview of the rapidly developing field of the current state of research on in vivo use of hypochlorous acid (HOCl) to aid infection prevention and control, including naso-pharyngeal, alveolar, topical, and systemic HOCl applications. Also, examples are provided of dedicated applications in COVID-19. A brief background of HOCl's biological and chemical specifics and its physiological role in the innate immune system is provided to understand the effect of in vivo applications in the context of the body's own physiological defense mechanisms.
    Description: Es wird ein umfassender Überblick über den aktuellen Stand der Forschung zum In-vivo-Einsatz von hypochloriger Säure (HOCl) zur Infektionsprävention und -bekämpfung gegeben. Der Schwerpunkt liegt auf nasalen, alveolären und topischen Anwendungen. Außerdem werden Beispiele für spezielle Anwendungen bei COVID-19 vorgestellt. Um die Wirkung von HOCl im Zusammenwirken mit körpereigenen physiologischen Abwehrmechanismen zu verstehen, werden die biologischen und chemischen Besonderheiten von HOCl und seiner physiologischen Rolle im humanen Immunsystem erläutert
    Subject(s): hypochlorous acid ; in vivo application ; HOCl chemistry ; innate immune response ; tissue compatibility ; infection control ; inhalation ; wound care ; disinfection ; Hypochlorige Säure ; In-vivo-Anwendung ; HOCl-Chemie ; physiologische Immunantwort ; Gewebeverträglichkei ; Infektionsprävention ; Inhalation ; Wundpflege ; Desinfektion ; ddc: 610
    Language: English
    Type: article
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  • 41
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC03 /20230130/
    Publication Date: 2023-02-04
    Description: Purpose: To report an occult intraocular foreign body mimicking choroidal melanoma. Methods: Medical records and imagings of the patient were retrospectively reviewed.Case description: A 76-year-old male was referred to our ocular oncology clinic with a suspicious hyperpigmented retinal lesion in the left eye. Biomicroscopy showed aphakia and peripheral iridectomy in the left eye. Fundoscopy revealed a pigmented, slightly elevated lesion on the macula of the left eye surrounded by diffuse atrophy. B-scan ultrasonography showed a preretinal hyperechoic lesion with posterior shadowing. There was no choroidal mass in B-scan or optical coherence tomography (OCT) imaging. On further questioning, it was disclosed that the patient had been hit by an iron fragment in the left eye forty years ago.Conclusion: Choroidal melanoma is a vision- and life-threatening intraocular malignant tumour. Various neoplastic, degenerative, and inflammatory conditions can simulate choroidal melanoma. A previous history of penetrating ocular trauma should lead the surgeon to re-evaluate a diagnosis of melanoma.
    Subject(s): choroid hemorrhage ; choroid neoplasms ; choroidal neovascularization ; foreign bodies ; eye injuries ; Aderhautblutung ; Aderhautneoplasmen ; choroidale Neovaskularisation ; Fremdkörper ; Augenverletzungen ; ddc: 610
    Language: English
    Type: article
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  • 42
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC01 /20230117/
    Publication Date: 2023-01-18
    Description: Objective: The aim of this study was to determine the acceptance of Covid-19 vaccine among the Turkish adult population. Methods: A total of 2023 persons participated in this cross-sectional study between October 2020 and January 2021. The questionnaire, which was delivered via social media, was filled out by the participants over "Google Forms".Results: Questionnaire results showed that 68.7% of the participants might agree to vaccinated against COVID-19. According to univariate analysis, the age group of 50-59, urban residents, healthcare workers, non-smokers, and those with chronic diseases, those who were vaccinated against influenza, pneumonia, and tetanus were all willing to be vaccinated against COVID-19.Conclusions: It is very important to determine a community's willingness to be vaccinated against COVID-19 so that interventions can be made to solve related problems. Risk of exposure and importance of Prevention play a critical role in vaccination acceptance.
    Description: Zielsetzung: Ziel der Studie war es, die Akzeptanz der Covid-19 Impfung in der türkischen Erwachsenenbevölkerung zu ermitteln.Methode: An der Querschnittsstudie nahmen zwischen Oktober 2020 und Januar 2021 2023 Personen teil. Der Fragebogen, der über soziale Medien übermittelt wurde, wurde von den Teilnehmern über "Google Forms" ausgefüllt.Ergebnisse: 68,7% der Teilnehmer stimmen der Impfung gegen COVID-19 zu. Die univariate Analyse ergab, dass die Altersgruppe der 50- bis 59-Jährigen, Stadtbewohner, Beschäftigte im Gesundheitswesen, Nichtraucher und chronisch Kranke sowie diejenigen, die gegen Influenza, Lungenentzündung und Tetanus geimpft waren, alle bereit waren, sich gegen COVID-19 impfen zu lassen.Schlussfolgerung: Es ist wichtig, die Bereitschaft einer Gemeinschaft zu ermitteln, sich gegen COVID-19 impfen zu lassen, damit Maßnahmen zur Lösung der damit verbundenen Probleme ergriffen werden können. Das Expositionsrisiko und die Erkennung von Gefahren spielen eine entscheidende Rolle für die Akzeptanz der Impfung.
    Subject(s): Covid-19 ; vaccine ; risk ; protectionimmunization ; Covid-19 ; Impfstoff ; Risiko ; Schutz ; Impfung ; ddc: 610
    Language: English
    Type: article
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  • 43
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC02 /20230123/
    Publication Date: 2023-01-24
    Description: The approval of ethanol by the Biocidal Products Regulation has been under evaluation since 2007 due to controversial opinions on the risk assessment. Because of this critical situation, 2022 a memorandum was published to verify whether the use of ethanol for hand antisepsis poses any hazard. On the basis of the memorandum a toxicological evaluation of ethanol-based hand rubs is given.
    Description: Die Bewertung von Ethanol als Biozid gemäß der Biozid-Verordnung wird seit 2007 aufgrund kontroverser Meinungen zur Risikobewertung geprüft. Wegen dieser kritischen Situation wurde 2022 ein Memorandum veröffentlicht, um zu überprüfen, ob die Verwendung von Ethanol zur Händedesinfektion eine Gefahr darstellt. Auf der Grundlage des Memorandums wird eine zusammenfassende toxikologische Bewertung Ethanol basierten Händedesinfektionsmittel gegeben.
    Subject(s): biocidal product regulation ; 1-propanol ; 2-propanol ; ethanol ; ethanol based hand rubs ; benefit-risk-assessment ; virucidal efficacy ; dermal absorption ; worker safety ; patient safety ; Biozid-Verordnung ; 1-Propanol ; 2-Propanol ; Ethanol ; Ethanol basierte Händedesinfektionsmittel ; Nutzen-Risiko-Bewertung ; Viruzidie ; dermale Resorption ; Mitarbeitersicherheit ; Patientensicherheit ; ddc: 610
    Language: English
    Type: article
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  • 44
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC04 /20230127/
    Publication Date: 2023-01-28
    Description: Objective: The risk of peripheral venous catheter (PVC) infections in inpatients is often underestimated, even if it is lower than that for central venous catheters. Guidelines for the prevention of PVC-associated infections describe the evidence-based management of PVCs. The aims of this study were the development of standardized methods for compliance assessment regarding PVC management and the evaluation of self-reported knowledge and implementations among healthcare providers regarding PVC care.Method: We developed a checklist based on the recommendation of the Commission of Hospital Hygiene and Infection Prevention at the Robert Koch Institute (KRINKO) Berlin for the standardized evaluation of PVC management. The following parameters were collected and evaluated: condition of the puncture site, condition of the bandage, presence of an extension set, presence of a plug, and documentation. The checklist was applied in 14 normal wards in 2019. After feedback of the ward staff on the results, it was applied again in 2020 in the same wards. For retrospective data analysis, we used a newly developed PVC-quality index. After the second evaluation in 2020, we carried out an anonymous survey among the healthcare providers.Results: The evaluation of 627 indwelling PVCs showed a significant increase in compliance related to the presence of an extension set (p=0.049) and documentation (p〈0.001) in the 2nd year. The quality index increased in 12 out of 14 wards. The participants of the survey were aware of the in-house standard "Prevention of vascular catheter-associated infections", with a mean score of 4.98 on a Likert scale (1=not aware, 7=completely aware). The main barrier to implementation of the preventive measures was the time factor. Survey participants were more aware of PVC placement than PVC care.Conclusion: The PVC quality index is a valuable tool for the assessment of compliance regarding PVC management in daily practice. Feedback from the ward staff on the results of compliance assessment improves PVC management, but the outcome is very heterogeneous.
    Description: Zielsetzung: Das Infektionsrisiko von peripheren Venenkathetern (PVK) bei stationären Patienten wird, auch wenn es niedriger ist als das von zentralen Venenkathetern, häufig unterschätzt. Leitlinien zur Prävention von PVK-assoziierten Infektionen beinhalten Evidenz-basierte Maßnahmen zur Anlage, Pflege und Nutzung von liegenden PVK. Ziel dieser Studie war, ein Verfahren zur standardisierten Bewertung der Compliance im Umgang mit liegenden PVK zu entwickeln und zu testen. Außerdem sollte eine Selbsteinschätzung des Personals zu Kenntnis und Umsetzung der Präventionsmaßnahmen erhoben werden.Methode: Basierend auf den KRINKO-Empfehlungen wurde eine Checkliste zur standardisierten Bewertung der Umsetzung von Präventionsmaßnahmen bei liegenden PVK entwickelt. Folgende Parameter wurden erhoben und bewertet: Zustand der Punktionsstelle, Zustand des Verbands, Vorhandensein eines Extensionssets, Verschluss, Nutzung und Dokumentation der Maßnahmen. Die Checkliste wurde erstmals im Jahr 2019 auf 14 Normalstationen angewendet und nach Feedback der Ergebnisse erneut im Jahr 2020 auf denselben Stationen eingesetzt. Mithilfe eines neu entwickelten PVK-Qualitätsindex erfolgte ein retrospektiver Vergleich. Nach der 2. Bewertung wurde eine anonyme Mitarbeiterbefragung mittels Fragebogen durchgeführt.Ergebnisse: Bei der Bewertung von 627 liegenden PVK zeigte sich eine signifikante Steigerung der Compliance bezogen auf das Vorhandensein eines Extensionssets (p=0,049) und die Dokumentation (p〈0,001) im 2. Jahr. Der Qualitätsindex ist auf 12 der 14 beobachteten Stationen gestiegen. Die Bekanntheit einzelner Maßnahmen des hausinternen Standards "Prävention gefäßkatheterassoziierter Infektionen" wurde mit dem Mittelwert 4,98 von 7 auf einer Likert-Skala angegeben; als einziges Hindernis in der Umsetzung konnte der Faktor Zeit identifiziert werden. Maßnahmen bei PVK-Anlage waren zu einem höheren Anteil bekannt als Maßnahmen der PVK-Pflege. Fazit: Der PVK-Qualitätsindex ist erfolgreich angewendet worden und ist geeignet, die PVK-bezogene Compliance standardisiert zu erheben. Feedback der Compliance im Umgang mit PVK hat diese verbessert, aber der Effekt ist sehr heterogen.
    Subject(s): catheter related infection ; peripheral venous catheter ; compliance assessment ; compliance self-assessment ; peripheral venous catheter quality index ; Katheter-assoziierte Infektion ; peripherer Venenkatheter ; Compliance-Bewertung ; Compliance-Selbsteinschätzung ; PVK-Qualitätsindex ; ddc: 610
    Language: English
    Type: article
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  • 45
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC03 /20230127/
    Publication Date: 2023-01-28
    Description: Introduction: There is a risk of transmission of viruses and microbial pathogens during routine health care procedures due to improper injection, infusion, and medication-vial practices. Unsafe practices lead to outbreaks of infection resulting in unacceptable and devastating events in patients. The present study was undertaken to assess the compliance of nurses with safe injection and infusion practices in our hospital and to identify staff education requirements in relation to the safe-injection and infusion practices policy.Methods: Baseline data were collected and high risk areas were identified on this basis, a quality improvement project was implemented by infection control team. FOCUS PDCA methodology was used to conduct the improvement process. The study was performed from March to September 2021. An audit checklist based on the CDC guidelines was used for monitoring compliance with safe injection and infusion practices. Results: Poor compliance with safe injection and infusion practices in few clinical areas at baseline. During the pre-intervention period, non-compliance was mainly seen with the following elements: aseptic technique (79%), rubber septum disinfected with alcohol (66%), labelling of all IV lines and medications with date and time (83%), compliance with multidose-vial policy (77%), use of multidose vials for single patient (84%), safe disposal of sharps (84%), using trays instead of clothing/pockets to carry medications (81%).There was significant improvement in compliance with the following elements of safe injection and infusion practices in the post-intervention period: aseptic technique (94%), rubber septum disinfected with alcohol (83%), compliance with multidose-vial policy (96%), use of multidose vials for single patient only (98%), safe disposal of sharps (96%).Conclusion: Adherence to safe injection and infusion practices is very important to prevent outbreaks of infection in health care settings.
    Description: Einführung: Im Fall unsachgemäßer Injektion, Infusion und fehlerhaften Umgangs mit Medikamenten besteht das Risiko der Übertragung von Viren und Mikroorganismen. Fehlerhafte Praktiken können zum Ausbruch von Infektionen mit drastischen Folgen für die Patienten führen. Die vorliegende Studie wurde durchgeführt, um die Einhaltung sicherer Injektions- und Infusionspraktiken in unserem Krankenhaus durch das Pflegepersonal zu bewerten und den Schulungsbedarf des Personals zu ermitteln.Methoden: Die Analyse der Ausgangssituation ergab eine schlechte Einhaltung sicherer Injektions- und Infusionspraktiken in einigen klinischen Bereichen. In den identifizierten Bereichen mit hohem Risiko wurde vom Infektionskontrollteam ein Qualitätsverbesserungsprojekt etabliert. Zur Durchführung wurde die FOCUS PDCA-Methode verwendet. Die Studie erstreckte sich von März bis September 2021. Zur Überwachung der Einhaltung sicherer Injektions- und Infusionspraktiken wurde eine Audit-Checkliste auf der Grundlage der CDC-Richtlinien verwendet.Ergebnisse: Während der Zeit vor der Intervention wurde die Nichteinhaltung hauptsächlich in Bezug auf folgende Elemente festgestellt: Aseptische Technik (79%), mit Alkohol desinfiziertes Gummiseptum (66%), Kennzeichnung aller Infusionsleitungen und Medikamente mit Datum und Uhrzeit (83%), Einhaltung der Richtlinie für Mehrdosisfläschchen (77%), Verwendung von Mehrdosisfläschchen für einen einzelnen Patienten (84%), sichere Entsorgung von scharfen Gegenständen (84%), Tragen von Medikamenten in Kleidung/Taschen anstatt auf dem Tablett (81%).Es gab eine signifikante Verbesserung bei der Einhaltung der folgenden Elemente sicherer Injektions- und Infusionspraktiken nach der Intervention: Aseptische Technik (94%), mit Alkohol desinfiziertes Gummiseptum (83%), Einhaltung der Richtlinie für Mehrdosis-Durchstechflaschen (96%), Verwendung von Mehrdosis-Durchstechflaschen für einen einzelnen Patienten (98%), sichere Entsorgung scharfer Gegenstände (96%).Schlussfolgerung: Die Einhaltung sicherer Injektions- und Infusionspraktiken ist unerlässlich, um Ausbrüche nosokomialer Infektionen zu verhindern.
    Subject(s): safe injection and infusion practices ; aseptic techniques ; single syringe ; single needle ; single patient ; multidose-vial policy ; labelling of IV lines ; safe disposal of sharps ; sichere Injektions- und Infusionspraktiken ; aseptische Technik ; Einzelspritze ; Einzelnadel ; Einzelpatient ; Mehrdosisfläschchen ; Kennzeichnung von Infusionsleitungen ; sichere Entsorgung scharfer Gegenstände ; ddc: 610
    Language: English
    Type: article
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  • 46
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC05 /20230130/
    Publication Date: 2023-01-31
    Description: Purpose: Vicarious menstruation is cyclical bleeding in extra-uterine locations that occurs during menstruation or within 48 h of its onset. We aim to present a 43-year-old female with ocular vicarious menstruation, its treatment, and a review of other published cases of ocular vicarious menstruation. Case presentation: A 43-year-old Caucasian female presented with a 15-year history of recurrent monthly unilateral subconjunctival hemorrhage. The episodes were cyclical and coincided with the onset of menses, lasting for approximately 10 to 14 days. Slit-lamp examination of the right eye showed nasally located subconjunctival hemorrhage. Detailed laboratory findings, including parameters for various hematological disorders, were normal. A follow-up examination 2 weeks later showed that the subconjunctival hemorrhage in the right eye was completely resolved. The patient was prescribed the oral contraceptive levonorgestrel/ethinyl estradiol and marked improvement at the recurrences of subconjunctival hemorrhage was noted during subsequent menses.Conclusion: Ocular vicarious menstruation is among the rarest causes of recurrent subconjunctival hemorrhage. A therapeutic trial of oral contraceptive should be considered in patients that present with ocular vicarious menstruation.
    Subject(s): recurrent subconjunctival hemorrhage ; ocular vicarious menstruation ; extrauterine bleeding ; oral contraceptive pills ; ddc: 610
    Language: English
    Type: article
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  • 47
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC04 /20230130/
    Publication Date: 2023-01-31
    Description: Objective: Surgically induced scleral necrosis (SISN) is a potentially blinding sequela that may occur after any ocular procedure. SISN in the context of active tuberculosis is seldom seen. We report a case of a patient with asymptomatic tuberculosis who developed SISN after pterygium surgery.Methods: A 76-year-old Mexican-mestizo woman from Veracruz, Mexico, was referred to our clinic because of severe disabling pain and scleral thinning in her right eye.Results: Tubercular-related SISN was finally diagnosed and managed successfully with antitubercular therapy, topical and systemic corticosteroidsConclusion: Tuberculosis must be considered as a differential diagnosis of high-risk patients in the context of refractory SISN in endemic countries.
    Subject(s): antitubercular therapy ; infectious scleritis ; necrotizing scleritis ; ocular tuberculosis ; pterygium surgery ; scleral inflammation ; surgically induced scleral necrosis ; ddc: 610
    Language: English
    Type: article
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