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  • 2020-2020  (39)
  • 1990-1994  (512,690)
  • 1975-1979  (270,111)
  • 1970-1974  (227,045)
Collection
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 294 (1992), S. 466-478 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 317 (1993), S. 474-484 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    Unknown
    Simon & Schuster
    Call number: B310:41 ; B310:42
    ISBN: 9781471129391
    Signatur Availability
    B310:41 departmental collection or stack – please contact the library
    B310:42 departmental collection or stack – please contact the library
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  • 4
    Call number: QH324:50
    Keywords: DKFZ-publications
    Pages: xxi, 116 p.
    ISBN: 9781138336346
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    QH324:50 available
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  • 5
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels
    Abstract: This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 293 p. 46 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998456
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  • 6
    Keywords: Neurology ; Neurobiology ; Neurology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Integrative Biomarkers in Stroke -- Vascular-Related Biomarkers of Ischemic Stroke -- Generation and Applicability of Genetic Risk Scores (GRS) in Stroke -- Neuroplasticity Biomarkers in Experimental Stroke Recovery -- Methods of Mitochondrial and Redox Measurements in Ischemic Stroke -- Blood Biomarkers for Stroke Differentiation -- Laser-Capture Micro-Dissection for Measurement of Angiogenesis after Stroke -- Blood-Borne Biomarkers of Hypertension Predicting Hemorrhagic and Ischemic Stroke -- RNA Gene Expression to Identify the Etiology of Acute Ischemic Stroke: The Biomarkers of Acute Stroke Etiology (BASE) Study -- Neurotoxicity Biomarkers Assays Development -- Glutamate Receptor Peptides as Potential Neurovascular Biomarkers of Acute Stroke -- Antibodies to NMDA Receptors in Cerebral and Spinal Cord Infarctions -- Impaired Retinal Vasoreactivity as an Early Marker of Stroke Risk in Diabetes -- Imaging Biomarkers: Keys to Decision Making in Stroke -- Neuroimaging Methods for Acute Stroke Diagnosis and Treatment -- Ultrasound Assessments of Risk for TIA and Stroke in Vascular Surgery -- Preoperative and Intraoperative Markers of Cerebral Ischemia -- Time is Brain: the Prehospital Phase and the Mobile Stroke Unit -- Acute Clinical Intervention and Chronic Management of Cerebral Vascular Accident -- Trends in Biomarkers Development for Stroke
    Abstract: This volume presents the latest data on recent achievements in new and emerging technologies for stroke biomarkers and innovations in stroke assessment. The topics discussed in this book explore the role of upcoming biomarkers in different types of stroke, and explores techniques that will allow researchers to be more effective when approaching clinical management and patient care. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to ensure successful results in the laboratory. Cutting-edge and comprehensive, Stroke Biomarkers is a valuable resource for both experimental and clinical scientists interested in expanding their knowledge in the field of stroke research
    Pages: XVII, 425 p. 80 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493996827
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  • 7
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
    Abstract: The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
    Pages: XI, 247 p. 30 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998654
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  • 8
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook -- Immunophenotyping by Mass Cytometry -- Fluorescent Cell Barcoding for Immunophenotyping -- Immunophenotyping using Dried and Lyophilized Reagents -- Guidelines for Gating Flow Cytometry Data for Immunological Assays -- Strategies and Techniques for NK Cell Phenotyping -- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue -- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells -- Immunophenotyping of Human Regulatory T Cells -- Immunophenotyping of Human Innate Lymphoid Cells -- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis -- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles -- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells -- Quality Control of Immunophenotyping -- Immunophenotyping of Acute Myeloid Leukemia -- Immunophenotyping of Acute Lymphoblastic Leukemia -- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia -- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
    Abstract: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field
    Pages: X, 356 p. 94 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493996506
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  • 9
    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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  • 10
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example
    Abstract: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field
    Pages: XIV, 405 p. 76 illus., 66 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998180
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  • 11
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 12
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 13
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    Unknown
    Basel : Karger
    Associated volumes
    Keywords: Radiotherapy ; Neoplasms
    Notes: This is a series title. Single volumes available in ZB from 2006 - Former volumes in ZB see: QZ200Z:72.
    ISSN: 0071-9676
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  • 14
    Call number: 01-Beschaffungsw:70 ; M230:3/1 ; M230:3/2
    Pages: loose-leaf
    ISBN: 3-8073-0843-1
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    01-Beschaffungsw:70 departmental collection or stack – please contact the library
    M230:3/1 departmental collection or stack – please contact the library
    M230:3/2 departmental collection or stack – please contact the library
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  • 15
    Keywords: Antibodies ; Pharmacology ; Antibodies ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Overview of the Current ADC Discovery Landscape -- Pushing the Envelope: Advancement of ADCs Outside of Oncology -- Conjugations to Endogenous Cysteine Residues -- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies -- Transglutaminase-Mediated Conjugations -- Click Chemistry Conjugations -- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates -- Bridged Cysteine Conjugations -- Antibody Conjugations via Glycosyl Remodeling -- ADC Analysis by Hydrophobic Interaction Chromatography -- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs -- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction -- Analysis of ADCs by Native Mass Spectrometry -- High Resolution Characterization of ADCs by Orbitrap LCMS -- Conjugation Site Analysis by MS/MS Protein Sequencing -- Conjugation Site Analysis of Lysine-Conjugated ADCs -- Characterization of ADCs by Capillary Electrophoresis -- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry -- Purification of ADCs by Hydrophobic Interaction Chromatography -- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations -- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions -- Biophysical Methods for Characterization of Antibody Drug Conjugates -- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines -- LC/MS Methods for Studying Lysosomal ADC Catabolism -- Assessing ADC Plasma Stability by LC/MS Methods -- Determination of ADC Concentration by Ligand-Binding Assays
    Abstract: This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the €œáctivation barrieŕ€ when undertaking a new discipline, and provides a ́€œtoolbox́€ for the next generation of ADC scientists
    Pages: XI, 373 p. 138 illus., 109 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999293
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  • 16
    Keywords: Infectious Diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Tour de Herpes: Cycling through the Life and Biology of HSV-1 -- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology -- Herpes Simplex Virus Growth, Preparation, and Assay -- Engineering HSV-1 Vectors for Gene Therapy -- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- oHSV Genome Editing by Means of galK Recombineering -- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein -- CRISPR/Cas9-Based Genome Editing of HSV -- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH) -- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing -- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals -- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry -- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells -- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot -- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane -- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination -- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1 -- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
    Abstract: This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV
    Pages: XIV, 457 p. 60 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998142
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  • 17
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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  • 18
    Keywords: Microbial genetics ; Microbial genomics ; Bacteriology ; Microbial Genetics and Genomics ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples -- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health -- Detection, Isolation, and Characterization of Plasmids in the Environment -- Bacteriophage Isolation and Characterization: Phages of Escherichia coli -- Detection and Characterization of Transposons in Bacteria -- Measuring Plasmid Conjugation Using Antibiotic Selection -- Measuring Plasmid Conjugation Using Fluorescent Reporters -- Methods to Quantify DNA Transfer in Enterococcus -- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis -- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases -- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis -- First Biochemical Steps on Bacterial Transposition Pathways -- Natural Transformation in Escherichia coli -- Integron Identification in Bacterial Genomes and Cassette Recombination Assays -- Methods to Identify and Analyze Vesicle-Protected DNA Transfer -- Measuring Plasmid Stability in Gram-Negative Bacteria -- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems -- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements -- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan -- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS) -- MOBscan: Automated Annotation of MOB Relaxases -- Plasmid Typing and Classification -- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets -- Statistical Analysis of Accessory Genome -- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits -- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria -- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria -- Conjugative Assembly Genome Engineering (CAGE)
    Abstract: This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality
    Pages: XV, 413 p. 62 illus., 52 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998777
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  • 19
    Keywords: Proteins  ; Biotechnology ; Nanotechnology ; Protein Science ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Introduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins
    Abstract: This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field
    Pages: XIII, 331 p. 84 illus., 64 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998692
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  • 20
    Keywords: Biochemistry ; RNA-ligand interactions ; Protein Science ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Predicting Protein-Protein Interactions using SPRINT -- Automated Extraction and Visualization of Protein-Protein Interaction Networks and Beyond: A Text Mining Protocol -- Construction of Functional Protein Networks using Domain Profile Associations -- Reconstruction of Protein-Protein Interaction Networks using Homology-Based Search: Application to the Autophagy Pathway of Ageing in Podospora anserina -- Predicting Interacting Protein Pairs by Co-Evolutionary Paralog Matching -- A Web-Based Protocol for Inter-Protein Contact Prediction by Deep Learning -- Visual Analysis of Protein-Protein Interaction Docking Models using COZOID Tool -- Path-LZerD: Predicting Assembly Order of Multimeric Protein Complexes -- Embedding Alternative Conformations of Proteins in Protein-Protein Interaction Networks -- Informed Use of Protein-Protein Interaction Data: A Focus on the Integrated Interactions Database (IID) -- Generation and Interpretation of Context-Specific Human Protein-Protein Interaction Networks with HIPPIE -- Explore Protein-Protein Interactions for Cancer Target Discovery using the OncoPPi Portal -- Perform Pathway Enrichment Analysis using ReactomeFIViz -- De Novo Pathway Enrichment with KeyPathwayMiner -- De Novo Pathway-Based Classification of Breast Cancer Subtypes -- Vienna Graph Clustering -- On TD-WGcluster - Theoretical Foundations and Guidelines for the User -- An Introductory Guide to Aligning Networks using SANA, the Simulated Annealing Network Aligner
    Abstract: This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: XI, 286 p. 97 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998739
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  • 21
    Call number: ERW
    Edition: 7. Aufl.
    ISBN: 9783662603796
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    ERW in acquisition
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  • 22
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    Totowa, N.J. : Humana Press
    Keywords: Molecular Biology ; Genetic Techniques
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6037
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  • 23
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 24
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 25
    Keywords: Bacteriology ; Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing of Staphylococcus aureus -- Genetic Manipulations of Staphylococcal Chromosomal DNA -- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology -- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus -- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus -- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology -- Metabolomic Profiling of Staphylococcus aureus -- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells -- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging -- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options -- Animal Models for Drug Development for MRSA
    Abstract: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens
    Pages: XI, 268 p. 35 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998494
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  • 26
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Anesthesiology ; Neurosciences ; Anesthesiology ; Springer eBooks
    Description / Table of Contents: The Challenge of Accidental Awareness during General Anesthesia -- Impact of Anesthetics on Brain Electrical Activity and Principles of pEEG-Based Monitoring during General Anethesia -- Perioperative Monitoring of Autonomic Nervous Activity -- Monitoring Cerebral Oximetry by Near Infrared Spectroscopy (NIRS) in Anaesthesia and Critical Care: Progress and Perspectives -- Post-Traumatic Stress Disorder following Intraoperative Awareness -- Mechanisms of Action of Inhaled Volatile General Anesthetics: Unconsciousness at the Molecular Level -- Intravenous Hypnotic Agents: From Binding Sites to Loss of Consciousness -- Pharmacological Considerations for the Use of General Anesthetics in the Elderly -- Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies -- Postoperative Cognitive Function following General Anesthesia in Children -- The Challenge of Opioid-Free Anesthesia -- New Insights into the Pharmacology of Dexmedetomidine and Open Issues for Neurosurgical Procedures -- Transgenic Mouse Models, General Anesthetics and Alzheimer Disease: Findings from Preclinical Studies -- The Biochemical Basis of Delirium -- Postoperative Delirium and Postoperative Cognitive Dysfunction
    Abstract: This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology. Furthermore, chapters in this book cover technical topics such as preoperative monitoring of autonomic nervous activity; EEG based monitoring of general anesthesia; monitoring cerebral oximetry by near infrared spectroscopy; pharmacological issues of anesthesia interest; and optimization of perioperative course through the latest techniques such as the opioid-free anesthesia. Finally, this book offers a comprehensive and up-to-date discussion on the phenomenon of general anesthesia awareness and its potential psychological sequelae. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, General Anesthesia Research is a valuable resource for novice and expert anesthesiologists who want to learn more about the important advances made in this developing field
    Pages: XIV, 256 p. 15 illus., 14 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998913
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  • 27
    Keywords: Neurobiology ; Developmental Biology ; Neurosciences ; Laboratory animals ; Neurobiology ; Developmental Biology ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra -- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis -- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis -- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System -- Immunostaining of the Embryonic and Larval Drosophila Brain -- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila -- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling -- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update -- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain -- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila -- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development -- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum -- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium -- X-Ray Microscopy of the Larval Crustacean Brain -- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain -- Immunostainings in Nervous System Development of the Nematode C. elegans -- Methods in Brain Development of Molluscs -- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization -- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum -- In Situ Hybridization and Immunostaining of Xenopus Brain -- Morpholino Studies in Xenopus Brain Development -- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish -- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish -- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain -- Gene Silencing in Chicken Brain Development -- Transplantation of Neural Tissue: Quail-Chick Chimeras -- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development -- The Cre/Lox System to Assess the Development of the Mouse Brain -- In Utero Electroporation to Study Mouse Brain Development
    Abstract: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain
    Pages: XIV, 527 p. 116 illus., 96 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493997329
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  • 28
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures
    Abstract: This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research
    Pages: XV, 363 p. 72 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493976171
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  • 29
    Keywords: Mass spectrometry ; Proteomics ; Mass spectrometry ; Proteomics ; Springer eBooks
    Description / Table of Contents: Introduction to Mass Spectrometry-based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Comparing Peptide Spectra Matches Across Search Engines -- Calculation of False Discovery Rate for Peptide and Protein Identification -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Interpretation of Tandem Mass Spectra of Posttranslationally Modified Peptides -- Solution to Dark Matter Identified by Mass-tolerant Database Search -- Phosphoproteomics Profiling to Identify Altered Signaling Pathways and Kinase-targeted Cancer Therapies -- Mass Spectrometry Based Characterization of Ub- and UbL-modified Proteins -- Targeted Proteomics as a Tool for Quantifying Urine-based Biomarkers -- Data Imputation in Merged Isobaric Labelling-based Relative Quantification Datasets -- Clustering Clinical Data in R -- Review of Issues and Solutions to Data Analysis Reproducibility and Data Quality in Clinical Proteomics -- Review of Batch Effects Prevention, Diagnostics, and Correction Approaches -- Using the Object-oriented PowerShell for Simple Proteomics Data Analysis -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data
    Abstract: The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics. In contrast to the previous editions, this third edition aims to provide the most relevant computational methods, focusing on computational concepts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Data Analysis in Proteomics, Third Edition to ensure successful results in the further study of this vital field
    Pages: X, 445 p. 136 illus., 94 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493997442
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  • 30
    Keywords: Molecular Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent
    Abstract: This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field
    Pages: X, 300 p. 50 illus., 32 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998821
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  • 31
    Keywords: Biomedical Engineering ; Cell Biology ; Bioinformatics ; Biology—Technique ; Systems Biology ; Biological systems ; Biomedical Engineering/Biotechnology ; Cell Biology ; Computational Biology/Bioinformatics ; Biological Techniques ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB: Image Analysis & Brownian Motion -- Resolving the process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM)
    Abstract: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images
    Pages: X, 170 p. 69 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030223861
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  • 32
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    Totowa, NJ : Humana Press
    Keywords: Molecular Biology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6029
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  • 33
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 34
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    New York, NY : Elsevier
    Keywords: Biochemistry ; Enzymes
    Notes: This is a series title, single volumes see link below.
    ISSN: 1557-7988
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  • 35
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    Hoboken, NJ : Wiley
    Call number: QA276:91(3)
    Keywords: Data Interpretation, Statistical ; Statistical Theory and Methods
    Pages: 449 p.
    Edition: 3rd ed.
    ISBN: 9780470526798
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  • 36
    Keywords: Cell Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Purinergic Signaling -- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling -- Agonists and Antagonists for Purinergic Receptors -- Homology Modelling of P2X Receptors -- Using RNA Interference for Purinoceptor Knock Down in vivo -- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos -- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues -- Flow Cytometry of Membrane Purinoreceptors -- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation -- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking -- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer -- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy -- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor -- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse -- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging -- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels -- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader -- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes -- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells -- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages -- Automated Planar Patch-clamp Recording of P2X Receptors -- Controlling Engineered P2X Receptors with Light -- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment -- Assays to Measure Purinoceptor Pore Dilation -- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization -- Measuring Leukocyte Migration to Nucleotides -- Assessment of Cell Adhesion after Purinoceptor Activation
    Abstract: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines
    Pages: XII, 360 p. 82 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997176
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  • 37
    Keywords: Human Genetics ; Proteins  ; Human Genetics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies
    Abstract: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field
    Pages: XII, 445 p. 86 illus., 61 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998531
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  • 38
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Experimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Abstract: This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study
    Pages: XI, 286 p. 65 illus., 53 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997848
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  • 39
    Keywords: Neoplasms / diagnosis ; Lymphoma ; Neoplasms / therapy ; Hematologic Neoplasms ; Leukemia
    Notes: Please search for single volumes in "Reihe (alphabetisch)" or "Reihe (Stichwort)"
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  • 40
    Keywords: Leukemia / therapy ; Prognosis
    Notes: Last volumes with varying subtitle and editor.
    ISSN: 0949-7021
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  • 41
    Keywords: Hazardous Substances / toxicity
    Notes: Ceased with vol. 15(1999).
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  • 42
    facet.materialart.
    Unknown
    Königswinter : Petersberg Verlag
    Keywords: Arbeitsgemeinschaft der Großforschungseinrichtungen (Germany) ; Research institutes / Germany ; Research ; Germany
    Notes: Ceased with ed. 1995.
    ISSN: 0935-2236
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  • 43
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Notes: Exploiting an incremental and parallel processing scheme is useful to improve the performance of natural language generation systems. TAG-GEN is a TAG-based syntactic generator that realizes both principles. It is shown how the demands of incremental and parallel generation influence the definition, the design, and the processing of syntactic rules on the basis of tree-adjoining grammars.
    Type of Medium: Electronic Resource
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  • 44
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Notes: Natural-language generation has gained importance since AI systems have become more sophisticated and canned text can no longer serve the elaborate communicative tasks in such a system. In this paper, suggestions are presented to apply uniformly the formalism of tree-adjoining grammar (TAG) for all tasks of natural-language generation. Up to now plan-based systems have been preferred for most of the processing during generation. Since the individual tasks of natural-language generation are very different and complex; for example, the determination of the propositional structure of a text is described using the TAG formalism–extended by constraints and feature descriptions (UCTAC). Nevertheless, this paper proposes basic ideas for the generalization of applying a grammar formalism to natural-language generation in order to build an overall integrated system for natural-language generation. Finally, advantages of such a uniform model are discussed.
    Type of Medium: Electronic Resource
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  • 45
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 46
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Notes: This paper presents an evaluation of a heuristic for partial-order planning, known as temporal coherence. The temporal coherence heuristic was proposed by Drummond and Currie as a method to improve the efficiency of partial-order planning without losing the ability to find a solution (i.e., completeness). It works by using a set of domain constraints to prune away plans that do not “make sense,” or are temporally incoherent. Our analysis shows that, while intuitively appealing, temporal coherence can only be applied to a very specific implementation of a partial-order planner and still maintain completeness. Furthermore, the heuristic does not always improve planning efficiency; in some cases, its application can actually degrade the efficiency of planning dramatically. To understand when the heuristic will work well, we conducted complexity analysis and empirical tests. Our results show that temporal coherence works well when strong domain constraints exist that significantly reduce the search space, when the number of subgoals is small, when the plan size is not too large, and when it is inexpensive to check each domain constraint.
    Type of Medium: Electronic Resource
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  • 47
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Notes: The frequent and conventional use of nonliteral language has been a major stumbling block for natural language processing systems since the early machine translation efforts. Metaphor, metonymy, and indirect speech acts are among the most troublesome phenomena. Recent computational efforts addressing these problems have taken an approach that emphasizes the use of systematic knowledge about nonliteral language conventions. We are currently engaged in an effort to supply this knowledge in the case of conventional metaphor. We are constructing MetaBank: an empirically derived and theoretically motivated knowledge-base of English metaphorical conventions. This article describes our three-part approach to the construction of MetaBank: the collection of on-line textual resources and databases of linguistic generalizations, the development of a methodology for analyzing these resources, and the construction of a knowledge-base based on the preceding analyses.
    Type of Medium: Electronic Resource
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  • 48
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 49
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 50
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 51
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 10 (1994), S. 0 
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 52
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 53
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Notes: For many physical processes it is extreme levels which are of greatest concern. This article gives a practical introduction to the problems and models involved.
    Type of Medium: Electronic Resource
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  • 54
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 55
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 56
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 57
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Notes: The instructor can help begining students master the numerous results of regression analysis by using helpful notation and by providing a framework for organising regression outputs. For the latter, model comparison is a useful operating notion.
    Type of Medium: Electronic Resource
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  • 58
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Notes: Several estimates of an unknown population size are compared.
    Type of Medium: Electronic Resource
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  • 59
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 60
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 16 (1994), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Notes: This work demonstrates common elements correlation, its extension to negative correlation, and the production of bivariate normal samples for a specified correlation matrix.
    Type of Medium: Electronic Resource
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  • 61
    ISSN: 0550-3213
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 62
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Physics, Section B 421 (1994), S. 3-37 
    ISSN: 0550-3213
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 63
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Physics, Section B 418 (1994), S. 403-427 
    ISSN: 0550-3213
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 64
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In most soft-rotting Erwinia spp., including E. carotovora sub sp. carotovora strain 71 (Ecc71), production of the plant cell wall degrading enzyme pectin lyase (PnI) is activated by DNA-damaging agents such as mitomycin C (MC). Induction of PnI production in Ecc71 requires a functional recA gene and the rdg locus DNA sequencing and RNA analyses revealed that the rdg locus contains two regulatory genes, rdgA and rdgB, in separate transcriptional units. There is high homology between RdgA and repressers of lambdoid phages, specially φ80. RdgB, however, has significant homology with transcriptional activators of Mu phage. Both RdgA and RdgB are also predicted to possess helix-turn-helix motifs. By replacing the rdgB promoter with the IPTG-inducible tac promoter, we have determined that rdgB by itself can activate PnI production in Escherichia coli. However, deletion analysis of rdg+ DNA indicated that, when driven by their native promoters, functions of both rdgA and rdgB are required for the induction of pnIA expression by MC treatment. While rdgB transcription occurs only after MC treatment, a substantial level of rdgA mRNA is detected in the absence of MC treatment. Moreover, upon induction with MC, a new rdgA mRNA species, initiated from a different start site, is produced at a high level. Thus, the two closely linked rdgA and rdgB genes, required for the regulation of PnI production, are expressed differently in Ecc71.
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  • 65