Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2020-2023  (465,814)
Collection
Language
Years
Year
  • 1
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Genetics. ; Genetics.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
    Type of Medium: Online Resource
    Pages: IX, 193 p. 37 illus., 34 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071624333
    Series Statement: Methods in Molecular Biology, 2519
    Language: English
    Note: A Basic and Simple Chromosome Preparation Protocol -- Metaphase chromosome preparation and classification of chromosomal aberrations -- Mitotic Index Analysis -- Preparation of Mitotic Cells for Fluorescence Microscopy -- Chemical induced Premature Chromosome Condensation protocol -- Apoptosis Detection Assays -- Alkaline Comet Assay To Detect DNA Damage -- Analysis for Sister Chromatid Exchange -- Cytokinesis Blocked Micronuclei Aberration Analysis -- DNA Damage Foci on Metaphase Chromosomes -- FISH with Whole Chromosome Painting Probes -- Telomere aberration detection by PNA-FISH probe -- Nontraditional Method for Telomere Staining by PNA Probes -- Visualizing Active Replication Regions in S-phase Chromosomes -- Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer -- CUT&Tag using "stress-free" con A-conjugated Dynabeads® -- Live-cell Synthetic Histone Acetylation by Chemical Catalyst -- Histone Modification Analysis of Low-Mappability Regions.
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Epigenetics. ; Biology—Technique. ; Genomics. ; Epigenetics. ; Genomic Analysis. ; Genomics.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.
    Type of Medium: Online Resource
    Pages: XI, 296 p. 75 illus., 64 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627242
    Series Statement: Methods in Molecular Biology, 2577
    Language: English
    Note: DNA Methylation Analysis Using Bisulfite Pyrosequencing -- Post-Bisulfite Adaptor Tagging Based on an ssDNA Ligation Technique (tPBAT) -- Reduced Representation Bisulfite Sequencing (RRBS) -- Chromatin Immunoprecipitation Sequencing (ChIP-seq) for Detecting Histone Modifications and Modifiers -- ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps to Data Analysis -- Low-Input CUT&RUN for Mouse Oocytes and Preimplantation Embryos -- Imaging Chromatin Accessibility by Assay of Transposase-Accessible Chromatin with Visualization -- STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes -- Bioinformatics Pipelines for Identification of Super-Enhancers and 3D Chromatin Contacts -- A Method for Detection of Somatic LINE-1 Insertions at the Single Cell Level from Postmortem Human Brain -- Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein -- Efficient Targeted DNA Methylation with dCas9-Coupled DNMT3A-DNMT3L Methyltransferase -- Regulation of Gene Expression Using dCas9-SunTag Platforms -- Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenous Mammalian Genes -- Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells -- A Split CRISPR–Cpf1 Platform for Inducible Gene Activation -- Targeted DNA Methylation in Mouse Early Embryos -- Generation of Epigenetic Disease Model Mice by Targeted Demethylation of the Epigenome -- In Vivo Tissue-Specific DNA Demethylation in Mouse Liver through a Hydrodynamic Tail Vein Injection -- Targeted Manipulation of Histone Modification in Medaka Embryos.
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Subject(s): Cytology. ; Proteins. ; Metabolism. ; Cell Biology. ; Proteins. ; Metabolism.
    In: Springer Nature eBook
    Description / Table of Contents: This thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as well. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure success in future research. Authoritative and practical, Cell-Wide Identification of Metabolite-Protein Interactions will aid researchers seeking a better understanding of the mechanisms of signal transduction occurring in the cell and assessing the effect of complex formation on cell physiology.
    Type of Medium: Online Resource
    Pages: X, 260 p. 29 illus., 24 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626245
    Series Statement: Methods in Molecular Biology, 2554
    Language: English
    Note: Protein-Metabolite Interactions Shape Cellular Metabolism and Physiology -- Affinity Purification Protocol Starting with a Small Molecule as Bait -- Cellular Thermal Shift Assay for the Detection of Small Molecule-Target Interactions in Arabidopsis Cells -- Solvent-Induced Protein Precipitation for Drug Target Discovery -- Identification of Plant Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectrometry (LiP-MS) -- Limited Proteolysis-Mass Spectrometry to Identify Metabolite-Protein Interactions -- Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution with Increased Statistical Significance and Reduced Sample Amount -- Parallel Analysis of Protein–Protein and Protein–Metabolite Complexes Using a Single-Step Affinity Purification -- Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) Spectroscopy -- PROMIS: Co-Fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions -- Metabolomics: Going Deeper, Going Broader, Going Further -- Specifics of Metabolite-Protein Interactions and Their Computational Analysis and Prediction -- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions -- Databases and Tools to Investigate Protein-Metabolite Interactions.
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Immunology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
    Type of Medium: Online Resource
    Pages: XI, 280 p. 52 illus., 42 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626474
    Series Statement: Methods in Molecular Biology, 2559
    Language: English
    Note: Analysis of the in vivo function of follicular regulatory T (TFR) cells in the regulation of antibody response -- Analysis of peripherally derived Treg in the intestines -- Isolation and analysis of tumor infiltrating Treg -- Induction of peripheral Treg -- Adoptive transfer and bone marrow chimera models to analyse Treg function and differentiation -- Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4+ T cells -- Genetic Tools for Analyzing Foxp3+ Treg cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping -- Depletion of Treg by the Diphtheria Toxin System -- Analysis of FOXP3 DNA methylation patterns to identify functional FOXP3+ T-Cell subpopulations -- Protein Ubiquitination Assay -- Phenotypic and Functional Studies of Human Treg cell Subpopulations -- In vitro analysis of CTLA-4 mediated transendocytosis by Regulatory T cells -- Isolation and functional characterisation of regulatory CD4+ T cells from the inflamed joints of patients with rheumatoid arthritis -- A GMP protocol for the manufacture of regulatory T cells for clinical application -- Regulatory T-cell phenotyping using CyTOF -- High-dimensional single-cell profiling of tumor-infiltrating CD4+ regulatory T cells -- Single-cell transcriptome analysis of Treg.
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Subject(s): Neurosciences. ; Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume looks at classic and novel methods currently used by researchers to understand mood and anxiety disorders and foster precision medicine. Chapters in this book cover topics such as how the sucrose preference succeeds or fails as a measurement of anhedonia; fear conditioning in laboratory rodents; animal models for mania; rodent models for studying the impact of variation in early life mother-infant interactions on mood and anxiety; and prediction of susceptibility/resilience toward animal models of PTSD. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, accommodating the novel views on how the neurobiology of psychiatric disorders should be reconceptualized, Psychiatric Vulnerability, Mood, and Anxiety Disorders: Tests and Models in Mice and Rats is a valuable resource for all researchers interested in learning more about this important and developing field.
    Type of Medium: Online Resource
    Pages: XIII, 401 p. 69 illus., 38 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627488
    Series Statement: Neuromethods, 190
    Language: English
    Note: Animal Models of Mood and Anxiety Disorders: The Pursuit of Standardization and Recognition of the Complex Neurobiology of Human Mental Health -- The Open Field Test -- The Light Dark Box Test in the Mouse -- The Olfactory Bulbectomized Rodent Remains a Valuable Preclinical Model of Depression and Antidepressant Activity -- Marble-Burying in Mice -- How the Sucrose Preference Succeeds or Fails as a Measurement of Anhedonia -- A Vogel Conflict Test Using Food Reinforcement in Mice -- Fear Conditioning in Laboratory Rodents -- Learned Helplessness in Rodents -- Chronobiology of Mood States: Introducing Circadian Animal Models -- Telemetry in Rats and Mice: Methodological Considerations and Example Studies of Stress and Anxiety in Ground-Based Spaceflight Analogs -- Utility and Implementation of Oral Corticosteroid Exposure in Behavioral Neuroscience Research with Rodents -- Animal Models for Mania -- Social Behavior Testing in Mice: Social Interest, Recognition, and Aggression -- Rodent Models for Studying the Impact of Variation in Early Life Mother-Infant Interactions on Mood and Anxiety -- Principles of Recording Rodent Ultrasonic Vocalizations -- Development of a Selectively Bred Mouse Model of Dominance and Submissiveness: Technical Considerations -- Prediction of Susceptibility/Resilience Toward Animal Models of Post-Traumatic Stress Disorder (PTSD). .
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cytology. ; Pharmacology. ; Cell Biology. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
    Type of Medium: Online Resource
    Pages: XIV, 373 p. 89 illus., 68 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626719
    Series Statement: Methods in Molecular Biology, 2565
    Language: English
    Note: Rat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    Subject(s): Bioinformatics. ; Computational and Systems Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides protocols for computational, statistical, and machine learning methods that are mainly applied to the study of metabolic engineering, synthetic biology, and disease applications. These techniques support the latest progress in cross-disciplinary research that integrates the different scales of biological complexity. The topics covered in this book are geared toward researchers with a background in engineering, computational analytical, and modeling experience and cover a broad range of topics in computational and machine learning approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Computational Biology and Machine Learning for Metabolic Engineering and Synthetic Biology is a valuable resource for any researcher or scientist who wants to learn more about the latest computational methods and how they are applied toward the understanding and prediction of complex biology. .
    Type of Medium: Online Resource
    Pages: XII, 455 p. 160 illus., 133 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626177
    Series Statement: Methods in Molecular Biology, 2553
    Language: English
    Note: Challenges to Ensure a Better Translation of Metabolic Engineering for Industrial Applications -- Synthetic Biology Meets Machine Learning -- Design and Analysis of Massively Parallel Reporter Assays using FORECAST -- Modelling Protein Complexes and Molecular Assemblies using Computational Method -- From Genome Mining to Protein Engineering: A Structural Bioinformatics Route -- Creating De Novo Overlapped Genes -- Design of Gene Boolean Gates and Circuits with Convergent Promoters -- Computational Methods for the Design of Recombinase Logic Circuits with Adaptable Circuit Specifications -- Designing a Model-Driven Approach Towards Rational Experimental Design in Bioprocess Optimization -- Modeling Subcellular Protein Recruitment Dynamics for Synthetic Biology -- Genome-Scale Modeling and Systems Metabolic Engineering of Vibrio Natriegens for the Production of 1,3-Propanediol -- Application of GeneCloudOmics: Transcriptomics Data Analytics for Synthetic Biology -- Overview of Bioinformatics Software and Databases for Metabolic Engineering -- Computational Simulation of Tumor-Induced Angiogenesis -- Computational Methods and Deep Learning for Elucidating Protein Interaction Networks -- Machine Learning Methods for Survival Analysis with Clinical and Transcriptomics Data of Breast Cancer -- Machine Learning Using Neural Networks for Metabolomic Pathway Analyses -- Machine Learning and Hybrid Methods for Metabolic Pathway Modeling -- A Machine Learning Based Approach Using Multi Omics Data to Predict Metabolic Pathways.
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Subject(s): Regenerative medicine. ; Regenerative Medicine and Tissue Engineering.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.
    Type of Medium: Online Resource
    Pages: XVI, 393 p. 116 illus., 105 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071628393
    Series Statement: Methods in Molecular Biology, 2598
    Language: English
    Note: Cartilage Tissue Engineering: An Introduction -- Chondrocyte Isolation and Expansion -- Isolation of Chondrons from Hyaline Cartilage -- Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation -- Physioxic Culture of Chondrogenic Cells -- Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells -- Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation -- Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells -- The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans -- Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy -- Metabolomic Profiling to Understand Chondrocyte Metabolism -- Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes -- Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads -- Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis -- Modulation of MicroRNA Expression during In Vitro Chondrogenesis -- Purification and Isolation of Proteins from Hyaline Cartilage -- Histological Preparation and Evaluation of Cartilage Specimens -- Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology -- Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization -- Viral Gene Delivery in Chondrocytes -- Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy -- Self-Assembly Culture Model for Engineering Musculoskeletal Tissues -- Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor -- A Mouse Model of Acute Cartilage Injury and Repair -- Small-Animal Compression Models of Osteoarthritis -- Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM) -- Collagenase-Induced Osteoarthritis in Mice -- Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence.
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Proteins. ; Cytology. ; Proteins. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.
    Type of Medium: Online Resource
    Pages: XIV, 430 p. 86 illus., 66 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071627440
    Series Statement: Methods in Molecular Biology, 2582
    Language: English
    Note: CCN Proteins - Cellular Communication Network Factors: Expanding their Repertoire toward a New Concept -- Optimal CCN4 Immunofluorescence for Tissue Microarray -- Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes -- Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and Cells -- Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes -- Evaluation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance -- Protocols for Screening Peptides Binding to CCN Family Proteins and their Extended Utility -- Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism -- Analyses of the Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression -- Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network -- Utilizing Public Molecular Biological Databases for CCN Family Research -- Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation -- Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy -- The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues -- Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis -- Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins -- Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis -- Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1 -- Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models -- Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins -- Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products -- Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain and Loss of Function Approaches -- Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney -- Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Cytology. ; Immunology. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.
    Type of Medium: Online Resource
    Pages: XV, 262 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071628355
    Series Statement: Methods in Molecular Biology, 2597
    Language: English
    Note: A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy -- Remote Ischemic Pre-Conditioning (RIPC) -- Mouse Corneal Transplantation -- Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant -- Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade -- Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion -- In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor -- The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins -- Flow Cytometry Analysis of Immune Cell Responses -- Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence -- Protein Microarrays and Their Fabrication -- NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions -- Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions -- Isolation and Compositional Analysis of Glycosaminoglycans -- How to Design Peptides -- Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins -- Producing Biologics with Defined N-Glycosylation in Plants -- Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery.
    Signatur Availability
    BibTip Others were also interested in ...
  • 11
    Subject(s): Medical genetics. ; Musculoskeletal system. ; Therapeutics. ; Medical Genetics. ; Musculoskeletal system. ; Therapeutics.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book presents a comprehensive collection of state-of-the-art protocols on muscular dystrophy therapeutics, covering therapeutics using antisense oligonucleotides, gene replacement, genome editing, small molecules, stem cells, and antibodies. Written by leaders in the field, the volume explores techniques that are currently in use and are starting an exciting therapeutic revolution in muscular dystrophy. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Muscular Dystrophy Therapeutics: Methods and Protocols serves as an ideal resource to inspire readers and provide tips, strategies, and advice to develop new therapeutic technologies for this group of diseases.
    Type of Medium: Online Resource
    Pages: XV, 575 p. 98 illus., 50 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627723
    Series Statement: Methods in Molecular Biology, 2587
    Language: English
    Note: Current Strategies of Muscular Dystrophy Therapeutics: An Overview -- The Story of Viltolarsen: From Preclinical Studies to FDA Approval -- Rapid Freezing of Skeletal and Cardiac Muscles Using Isopentane Cooled with Liquid Nitrogen and Tragacanth Gum for Histological, Genetic, and Protein Expression Studies -- Cardiac and Skeletal Muscle Pathology in the D2/mdx Mouse Model and Caveats Associated with the Quantification of Utrophin -- Physiological Assessment of Muscle, Heart, and Whole Body Function in the Canine Model of Duchenne Muscular Dystrophy -- Restoring Dystrophin Expression by Skipping Exon 6 and 8 in Neonatal Dystrophic Dogs -- Restoring Dystrophin Expression with Exon 44 and 53 Skipping in the DMD Gene in Immortalized Myotubes -- Restoring Dystrophin Expression with Duchenne Muscular Dystrophy Exon 45 Skipping in Induced-Pluripotent Stem Cell-Derived Cardiomyocytes -- Quantitative Evaluation of Exon Skipping in Urine-Derived Cells for Duchenne Muscular Dystrophy -- Use of Glycine to Augment Exon Skipping and Cell Therapies for Duchenne Muscular Dystrophy -- Morpholino-Mediated Exons 28–29 Skipping in Dysferlin -- Knocking Down DUX4 in Immortalized Facioscapulohumeral Muscular Dystrophy Patient-Derived Muscle Cells -- Peptide-Conjugated PMOs for the Treatment of Myotonic Dystrophy -- Developing Therapeutic Splice-Correcting Antisense Oligomers for Adult-Onset Pompe Disease with c.-32-13T〉G Mutation -- Molecular and Biochemical Assessment of Gene Therapy in the Canine Model of Duchenne Muscular Dystrophy -- Histological Assessment of Gene Therapy in the Canine DMD Model -- MRI Evaluation of Gene Therapy in the Canine Model of Duchenne Muscular Dystrophy -- Assessment of the Gene Therapy Immune Response in the Canine Muscular Dystrophy Model -- Use of Mesenchymal Stem Cells to Enhance the Efficacy of Gene Therapy -- Exon-Skipping for a Pathogenic COL6A1 Variant in Ullrich CMD -- CRISPR-Cas9 Correction of Duchenne Muscular Dystrophy in Mice by a Self-Complementary AAV Delivery System -- Preparation of NanoMEDIC Extracellular Vesicles to Deliver CRISPR-Cas9 Ribonucleoproteins for Genomic Exon Skipping -- Restoration of Dystrophin Expression in Mdx-Derived Muscle Progenitor Cells Using CRISPR/Cas9 System and Homology-Directed Repair Technology -- Effects of Glucocorticoids in Murine Models of Duchenne and Limb-Girdle Muscular Dystrophy -- High-Throughput Screening to Identify Modulators of Sarcospan -- Identifying FDA-Approved Drugs that Upregulate Utrophin A as a Therapeutic Strategy for Duchenne Muscular Dystrophy -- Monitoring Membrane Injury-Triggered Endocytosis at Single Cell and Single Vesicle Resolution -- Evaluation of hiPSC-Derived Muscle Progenitor Cell Transplantation in a Mouse Duchenne Muscular Dystrophy Model -- Quantification of Muscle Satellite Stem Cell Divisions by High Content Analysis -- Systemic Delivery of a Monoclonal Antibody to Immunologically Block Myostatin in the A17 Mouse Model of OPMD.
    Signatur Availability
    BibTip Others were also interested in ...
  • 12
    Subject(s): Mineralogy. ; Forensic sciences. ; Soil science. ; Mineralogy. ; Forensic Science. ; Soil Science.
    In: Springer Nature eBook
    Description / Table of Contents: This book illustrates the main modern mineralogical analytical procedures that can be applied for forensic purposes on various typologies of materials and substances and has both theoretical and practical approach. Moreover, it focuses on all those challenges that can arise with forensic analysis, such as the choice of the most proper mineralogical techniques as a function of the material and its quantity, destructive and non-destructive analyses, sampling procedures, mineralogical analysis of micro-traces, correct preparation of the samples, correct calibration and analytical conditions of the laboratory instrumentation. Numerous case studies on criminal offenses against persons, environment and Cultural Heritage are illustrated. .
    Type of Medium: Online Resource
    Pages: XI, 311 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031088346
    Series Statement: Soil Forensics,
    Language: English
    Note: 1. Optical microscopy applied to forensics -- 2. X-ray powder diffraction (XRPD -- 3. Scanning Electron Microscopy, Electron Microanalysis and automated SEM for forensic science -- 4. Infrared spectroscopy -- 5. Raman spectroscopy and forensic mineralogy -- 6. ICP-MS for forensic science -- 7. Simultaneous Thermal Analysis (STA): a powerful tool for forensic purposes -- 8. X-ray fluorescence -- 9. Isotopic analysis techniques applied in forensics. New frontiers of isotope geochemistry -- 10. Image analysis in forensic mineralogy.
    Signatur Availability
    BibTip Others were also interested in ...
  • 13
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Proteins. ; Cytology. ; Proteins. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.
    Type of Medium: Online Resource
    Pages: XI, 326 p. 95 illus., 80 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626672
    Series Statement: Methods in Molecular Biology, 2564
    Language: English
    Note: Using FPbase: The Fluorescent Protein Database -- Selecting the Fluorescent Protein for In Vivo Imaging Experiments -- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo -- Directed Evolution of Fluorescent Proteins in Bacteria -- Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation -- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization -- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization -- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line -- Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants -- Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions -- DNA Visualization Using Fluorescent Proteins -- Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors -- Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out -- Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae -- Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells -- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins -- Determining Protein-Protein Interaction with GFP-Trap Beads.
    Signatur Availability
    BibTip Others were also interested in ...
  • 14
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Biochemical markers. ; Medicine—Research. ; Biology—Research. ; Metabolism—Disorders. ; Pharmacology. ; Biomarkers. ; Biomedical Research. ; Metabolic Disease. ; Pharmacology.
    In: Springer Nature eReference
    Description / Table of Contents: This handbook focuses specifically on biomarkers in diabetes and provides a comprehensive understanding of this field. Readers will gain deep insights into bioinformatics and network analysis of biomarkers in diabetes, and will learn about circulating biomarkers in body fluids and specific pathological features of diabetes. Various animal models in diabetes research are also presented. In addition, like the previous volumes in this large reference series, the book provides a comprehensive look at genetics, cellular, and histological variables. The goal of this handbook is to provide information on markers of this disease to facilitate diagnosis, introduce new technologies, and ultimately improve health. It is a must for researchers as well as advanced students and physicians in the field of diabetes and biomarker research and application.
    Type of Medium: Online Resource
    Pages: 139 illus., 108 illus. in color. eReference. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031080142
    Series Statement: Biomarkers in Disease: Methods, Discoveries and Applications,
    Language: English
    Note: Bioinformatics and biomarkers for diabetes -- Network analysis of biomarkers un diabetes.-Biomarkers of insulin action .-Circulating and body fluid biomarkers -glucose.-Fructosamine as a glycaemic marker: features and applications.-Postload plasma glucose and oral glucose tolerance tests (OGTT): applications -- Circulating and body fluid biomarkers -impact of diabetes on pathology -- Biomarkers of diet in relation to diabetes: 5-CIS-, trans-and total lycopene -- Fatty Acid Biomarkers and Type 2 Diabetes -- Biomarkers of retina-related disease in diabetes.-Biomarkers of diabetes-induced nephropathy -- Biomarkers of atherosclerosis in diabetes mellitus -- Advanced glycation end products as biomarkers in diabetes -- Biomarkers of vascular injury in diabetes -- Amino acids as a biomarkers in diabetes .-Adiponectin-leptin ratio and diabetes -- Plasma omentin-1 as a biomarker in type 2 diabetes -- Serpin A12 as a type 2 diabetes biomarker.-Circulating Hsp90 as a potential biomarker of beta cell autoimmunity in type 1 diabetes.-CUBN variants, HES1, CDC73 and GRM5 as biomarkers in diabetes.-Biomarker polymorphisms and diabetic patients -- Epigenetics and 5-Hydroxymethylcytosines as a biomarker in type 2 diabetes. - Longevity biomarkers and applications to diabetes. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 15
    Subject(s): Virology. ; Immunology. ; Human physiology. ; Vaccines—Biotechnology. ; Biomaterials. ; Virology. ; Immunology. ; Human Physiology. ; Biomaterials-Vaccines.
    In: Springer Nature eBook
    Description / Table of Contents: This edited volume provides insights about the molecular virology, pathoenesis of infection and host responses to varicella-zoster virus, which is a medically important human herpesvirus. The chapters not only discuss genetics and molecular evolution of the virus, but also new structure-based knowledge on the functions of viral glycoproteins. Further, the authors illustrate how mechanisms from the virus are used to take over and remodel host cells during pathogeneis. These mechanisms also enable viral modulation of host cells and evasion of the host immune response. Part of these evasion and survival strategies, is the reaction to triggered host cell responses like autophagy, the stress response, modulation of apoptosis and other cell death pathways. This hands-on knowledge is crucial for students and clinicians in Virology, Immunology, and Infectious Diseases. Chapter "Common features between stroke following varicella in children and stroke following herpes zoster in adults" is available open access under a Creative Commons Attribution 4.0 International License via Springerlink.com.
    Type of Medium: Online Resource
    Pages: VIII, 272 p. 39 illus., 32 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031153051
    Series Statement: Current Topics in Microbiology and Immunology, 438
    Language: English
    Note: Varicella-zoster Virus - Genetics, Molecular Evolution, and Recombination -- The structures and functions of VZV glycoproteins -- Modulation of apoptosis and cell death pathways by Varicella zoster virus -- Modulation of host cell signaling pathways by varicella-zoster virus -- Modulation of MHC and MHC-like molecules by Varicella Zoster Virus -- Varicella Zoster Virus Neuronal Persistence and Reactivation modeled in-vitro -- Humanized Severe Combined Immunodeficient (SCID) Mouse Models for Varicella-Zoster Virus Pathogenesis -- Simian Varicella Virus: Molecular Virology and mechanisms of pathogenesis -- A guide to preclinical models of zoster-associated pain and postherpetic neuralgia -- Immune Responses to Varicella-Zoster Virus Vaccines -- Common features between stroke following varicella in children and stroke following herpes zoster in adults.
    Signatur Availability
    BibTip Others were also interested in ...
  • 16
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Cancer. ; Stem cells. ; Cytology. ; Developmental biology. ; Cancer Stem Cells. ; Cell Biology. ; Developmental Biology and Stem Cells.
    In: Springer Nature eBook
    Description / Table of Contents: This book covers a wide range of topics that illustrate the various functions of autophagy in stem cells and offers insights on the mechanisms by which autophagy can regulate stem-cell self-renewal and facilitate specific differentiation programs. Stem cells are unique cells present in most multicellular animals and are essential for their survival. They have two unique properties: the ability to self-renew and the ability to differentiate into one or more cell types. These characteristics of stem cells have found immense therapeutic potential in regenerative medicine. Autophagy is a crucial membrane trafficking pathway that is essential for maintaining cellular homeostasis that involves sequestration of non-functional proteins, protein aggregates and damaged organelles in double-membraned vesicles called autophagosomes, which are subsequently targeted to the lysosome for degradation. The primary aim of this book is to provide knowledge of recent developments in our understanding of the role of autophagy in stem cells, including germline stem cells. Autophagy is considered a promising target for many diseases. Significant efforts are being developed to identify specific modulators of autophagy, which will aid in designing combinatorial therapeutic strategies that will allow significant improvements in regenerative medicine.
    Type of Medium: Online Resource
    Pages: VIII, 288 p. 41 illus., 34 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031173622
    Series Statement: Stem Cell Biology and Regenerative Medicine, 73
    Language: English
    Note: Assays for monitoring autophagy in stem cells -- Autophagy in Stem Cell Maintenance and Differentiation -- Autophagy in Embryonic stem cells and Neural stem cells -- Autophagy in Germline Stem Cells -- The role of autophagy in the regulation of hematopoietic stem cells -- Autophagy in Muscle Stem Cells -- Autophagy in Intestinal Stem Cells -- The autophagy lysosomal pathway: friend or foe in adult neural stem cells? -- Autophagy in Mesenchymal Stem Cell-based therapy -- Autophagic control of stem cells differentiation into osteogenic lineage- Implications in bone disorders -- Autophagy in Cancer Metastasis.
    Signatur Availability
    BibTip Others were also interested in ...
  • 17
    Subject(s): Neurosciences. ; Computational neuroscience. ; Biophysics. ; Science—History. ; Neuroscience. ; Computational Neuroscience. ; Biophysics. ; History of Science.
    In: Springer Nature eBook
    Description / Table of Contents: This book brings together a selection of papers by George Gerstein, representing his long-term endeavor of making neuroscience into a more rigorous science inspired by physics, where he had his roots. Professor Gerstein was many years ahead of the field, consistently striving for quantitative analyses, mechanistic models, and conceptual clarity. In doing so, he pioneered Computational Neuroscience, many years before the term itself was born. The overarching goal of George Gerstein’s research was to understand the functional organization of neuronal networks in the brain. The editors of this book have compiled a selection of George Gerstein’s many seminal contributions to neuroscience--be they experimental, theoretical or computational--into a single, comprehensive volume .The aim is to provide readers with a fresh introduction of these various concepts in the original literature. The volume is organized in a series of chapters by subject, ordered in time, each one containing one or more of George Gerstein’s papers.
    Type of Medium: Online Resource
    Pages: XIII, 548 p. 253 illus., 29 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783030874476
    Series Statement: Springer Series in Computational Neuroscience,
    Language: English
    Note: 1. Integrate and fire: RC circuits to model neurons and reward monkeys -- 2. Neuronal spike trains, stochastic point processes, and why George Gerstein’s papers are still worth reading -- 3. Visual Functions of Inferior Temporal Cortex -- 4. Early Days in the Gerstein Lab: Neuronal Plasticity in 1, 2, and 3 Dimensions -- 5. Patterns in Spike Trains -- 6. Introduction to the Gravity Papers -- 7. On correlations and interactions between neurons and with George Gerstein -- 8. Significance of Correlations between Spike Trains -- 9. Spatial-Temporal Spike Patterns -- 10. Does Cross Correlation Have a Unique Interpretation? -- 11. Neuronal Plasticity and my Experience Working with George L. Gerstein -- 12. Auditory Experiments and Modeling -- 13. Thinking about Analysis Methods with George in Philadelphia, Cambridge and Newcastle -- 14. Higher-order Correlations and Synfire Chains -- Nencki Award for George L. Gerstein.
    Signatur Availability
    BibTip Others were also interested in ...
  • 18
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Immunology. ; Food science. ; Nutrition   . ; Immunology. ; Food Science. ; Nutrition.
    In: Springer Nature eBook
    Description / Table of Contents: Nutrition is an important environmental factor for the maturation of the human immune system and essential for maintaining immunological homeostasis. Based on this, a variety of food applications with medical claims are being generated by food manufacturers worldwide in order to expand the market potential of products creating interesting linkages with other market segments, such as cosmetics and pharmaceuticals. However, in addition to the health benefits, active principles of such components often remain unexplored. This book focusses on the specific interactions between food ingredients and the immune system along the entire immune defense response. Starting from the immune barrier, through the innate and adaptive immune response, to active limitation and termination, all major mechanisms of the immune response are addressed and different biochemical, cellular and genetic interactions of components of our diet are discussed. The book presents a wealth of disease patterns for which nutritional factors are relevant and thereby provides indications for potential intervention strategies. In addition, associated food-technological aspects are discussed. Being the first of its kind, this book provides an overview of the variety of functional food components and their influence on immunological responses. Written in an accessible style, it addresses researchers, health professionals and students with different scientific backgrounds. This book is a translation of an original German edition. The translation was done with the help of artificial intelligence (machine translation by the service DeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation.
    Type of Medium: Online Resource
    Pages: XXIII, 241 p. 73 illus., 68 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031115233
    Language: English
    Signatur Availability
    BibTip Others were also interested in ...
  • 19
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Food science. ; Food—Analysis. ; Chemistry. ; Chemistry, Organic. ; Food Science. ; Food Chemistry. ; Organic Chemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This useful and extensive set of guidelines is designed to assist food control laboratories in gainingaccreditation from an internationally recognized external body, providing all of the necessary information and practices in an easy-to-read, step-by-step fashion. Authored by an experienced consultant for laboratory accreditation in many different countries, with this text food control lab owners now have all of the up-to-date information they need to gain accreditation in a single source. Guidelines for Laboratory Quality Managers covers the essentials for quality management in the food control laboratory, from testing processes to current quality management systems. The ISO standards for accreditation are extensively explored, including managerial requirements,organizational aspects, complaint handling procedures, internal audits, and sampling. An entire section is dedicated to the implementation of managerial and technical requirements from quality control to program monitoring and evaluation. Analysis selection, preparation, and validation is covered extensively, and an entire section is dedicated to basic statistics from data presentation todistribution. Each section comes with helpful tips for lab managers plus definitions and terms. Comprehensive, easy-to-use and up-to-date, Guidelines for Laboratory Quality Managers isthe guide for accreditation for food control laboratories. .
    Type of Medium: Online Resource
    Pages: XV, 160 p. 34 illus., 29 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031117244
    Series Statement: Integrating Food Science and Engineering Knowledge Into the Food Chain, 14
    Language: English
    Note: Laboratory management and ISO 17025:2017 -- Essential for Quality management in testing laboratories -- Preparing for analysis: the analytical method -- Statistics for the Quality Control Laboratory -- Uncertainty measurement -- Control Charts and Process capability -- Risk Management.
    Signatur Availability
    BibTip Others were also interested in ...
  • 20
    Subject(s): Evolution (Biology). ; Zoology. ; Anatomy. ; Evolutionary Biology. ; Paleontology. ; Zoology. ; Anatomy.
    In: Springer Nature eBook
    Description / Table of Contents: TThis book presents a detailed examination of the current state of knowledge in the field of paleoneurology in the main amniote groups (reptiles, birds and mammals), and advances resulting from new non-invasive technologies. The study of fossil endocasts is an area of considerable current interest, and has long been central to our understanding of the evolution of the brain, development of senses and behavioral adaptations in diverse vertebrate groups and across vertebrates as a whole. Recent advances in non-invasive imaging have significantly increased the number of fossil taxa for which brain morphology is known, and it may now be possible to quantitatively analyze the relative size of brain regions. Providing a general overview of current perspectives and problems in evolutionary neuroanatomy, this book is intended for a wide range of readers, including undergraduate and graduate students, teachers, and anyone with a special interest in paleoneurology. It is also useful as supplementary reading for courses in digital anatomy, vertebrate comparative anatomy, computed morphometrics, paleontology, neurology and radiology as well as evolution programs.
    Type of Medium: Online Resource
    Pages: XX, 840 p. 171 illus., 123 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031139833
    Language: English
    Note: PART 1: FOSSIL ENDOCASTS IN REPTILES AND BIRDS -- Chapter 1 - Onto land and back into water: basal diapsids and marine reptiles -- Chapter 2 - Sauropterygia and Ichthyopterygia: Paleoneurology of secondarily aquatic reptiles -- Chapter 3 - Contrasting brains and bones: implications for turtle paleoneurology -- Chapter 4 - Approaches to the Paleoneurology of Lepidosauromorpha.-Chapter 5 - Paleoneuroanatomy of Triassic archosauriforms and pseudosuchians -- Chapter 6 - Paleoneurology of Crocodylomorpha -- Chapter 7 - Neurosensory evolution in the pterosaurs -- Chapter 8 - Paleoneurology of dinosaur endocasts: past, present, and future research -- Chapter 9 - The evolution of bird brain -- PART 2: FOSSIL ENDOCASTS IN MAMMALS -- Chapter 10 - Paleoneurology of stem and basal Mammalia -- Chapter 11 - Evolution of the brain and sensory structures in monotremes/Evolution of the brain and sensory structures in marsupials -- Chapter 12 - Early evolution of the brain in Primates and their close kin -- Chapter 13 - The brain of condylarthra and extinct Cetartiodactyla -- Chapter 14 - Sirenia fossil endocranial casts: history and evolution -- Chapter 15 - Paleoneurology of the Proboscidea (Mammalia, Afrotheria): insight from the brain endocast, bony labyrinth and infraorbital canal -- Chapter 16 - North American and South American Fossil Rodents: Cranial endocasts of extinct rodents and brain evolution -- Chapter 17 - Parallel patterns in carnivore brain evolution/South American Fossil Carnivorans: Auditory Region of Ursidae and Cranial Endocast of Procyonidae -- Chapter 18 - The Cranial Endocast and Inner Ear Anatomy of Extinct Cingulates -- Chapter 19 - The endocranial cavities of Folivora (Mammalia, Xenarthra): insights from the brain cavity, bony labyrinth and cranial sinuses -- Chapter 20 - Cranial Endocasts and Inner Ear of Notostylopidae and Typotheria/Endocranial Morphology of Toxodontians (Mammalia, Notoungulata) and Brain Evolution in Paleogene Notoungulates -- Chapter 21 - Digital and Natural Cranial Endocasts of Macraucheniidae (Mammalia, Litopterna): Brain and Frontal Sinuses.
    Signatur Availability
    BibTip Others were also interested in ...
  • 21
    Online Resource
    Online Resource
    Singapore : Springer Nature Singapore
    Subject(s): Food science. ; Food—Analysis. ; Chemistry. ; Food Science. ; Food Analysis. ; Food Engineering. ; Food Chemistry.
    In: Springer Nature eBook
    Description / Table of Contents: The book covers different techniques and methodologies involved in the nutritional quality analysis of forages. It also discusses the nutritional quality, anti-nutritional components, factors affecting forage quality, feed processing and conservation. Different techniques and methodologies have been presented in a simplified manner. The book has been divided in separate chapters and each chapter discusses different aspect of forage quality. Further, the book also covers the topics on conservation and processing of forages and management techniques for improving the forage nutritional quality. This book is an essential source of information for research scholars, post-graduate students and scientists working on forage quality estimation and also in livestock and dairy industries.
    Type of Medium: Online Resource
    Pages: XVII, 233 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9789811960208
    Language: English
    Note: 1. Introduction -- 2. Forage quality vis-a vis livestock productivity -- 3. Forage Quality Indices -- 4. Forage quality components -- 5. Management of anti-nutrients in forages -- 6. General scheme for forage quality analysis -- 7. Laboratory essentials for forage evaluation -- 8. Solutions and buffers in estimations -- 9. Estimation of nutritional components in forages -- 10. Estimation of anti-quality and anti-nutritional factors in forages -- 11. Estimation of mineral content in forages -- 12. Estimation of phytochemicals and enzyme activities in forages -- 13. Quality Estimation of Natural Grasslands and Pastures. 14. Nutritional Quality of Important Forages -- 15. Conservation and processing of forages -- 16. Factors Affecting Forage Quality -- 17. Forage Nutritional Quality Management.
    Signatur Availability
    BibTip Others were also interested in ...
  • 22
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Virology. ; Virology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, West Nile Virus: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field.
    Type of Medium: Online Resource
    Pages: XI, 228 p. 36 illus., 28 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627600
    Series Statement: Methods in Molecular Biology, 2585
    Language: English
    Note: Introduction to West Nile Virus -- Quantification of West Nile virus by Plaque-Forming Assay -- Viral titer quantification of West Nile virus by immunostaining plaque assay -- Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection -- Isolation of Murine Bone Marrow Derived Neutrophils for Infection Modeling -- Methods to study West Nile Virus infection, and the virus-induced inflammation in the brain in a murine model -- Detection of West Nile virus envelope protein in brain tissue with an immunohistochemical assay -- Quantitative Analysis of B Cell Subpopulations in Bone Marrow by Flow Cytometry -- Isolation of exosomes or extracellular vesicles from West Nile Virus-infected N2a cells, Primary Cortical Neurons and Brain Tissues -- Protocol to Study West Nile Virus Infection in Brain Slices In Vitro -- Studying Virus-Host Interactions with CRISPR technology -- Protocol of detection of West Nile Virus in Clinical Samples -- Detection and Analysis of West Nile Virus Structural Protein Genes in Animal Or Bird Samples -- Mosquito surveillance for West Nile Virus -- Molecular surveillance of West Nile virus in mosquitoes and sentinel chickens -- Statistical tools for West Nile Virus Disease Analysis -- Analytical Approaches to Uncover Genetic Associations for Rare Outcomes: Lessons from West Nile Neuroinvasive Disease -- Safety procedures to work with West Nile virus in biosafety level 3 facilities -- Development of antibody-based therapeutics against West Nile virus in plants. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 23
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Genetics. ; Genetics.
    In: Springer Nature eBook
    Description / Table of Contents: This book aims to be an introduction to haplotype information for both the wet and dry labs. Chapters detail co-barcoding and linked-reads based methods, third generation sequencing based methods, Hi-C based methods, single-cell and Strand-seq, and methods for using haplotype data once obtained. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Haplotyping: Methods and Protocols aims to provide compréhensive and accessible methods to undergraduate, graduate, and established scientists. Chapters 2 and 8 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
    Type of Medium: Online Resource
    Pages: XI, 298 p. 50 illus., 40 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071628195
    Series Statement: Methods in Molecular Biology, 2590
    Language: English
    Note: Interrogating the human diplome: computational methods, emerging applications, and challenges -- Targeted Locus Amplification and Haplotyping -- Full length transcript phasing with third generation sequencing -- Large scale complete sequencing and haplotyping of 1-10 kb DNA molecules using short massively parallel reads -- Accurate sequencing and haplotyping from 10 cells using Long Fragment Read (LFR) Technology -- Low-cost genome-scale phasing with barcode-linked sequencing -- A simple cost-effective method for whole genome sequencing, haplotyping, and assembly -- Read-based phasing and analysis of phased variants with WhatsHap -- Hapcut2, a method for phasing genomes using experimental data -- Determining complete chromosomal haplotypes by mLinker -- Haplotyping-assisted diploid assembly and variant detection with linked-reads -- Chromosome-length haplotypes with StrandPhaseR and Strand-seq -- Gamete-binning to achieve haplotype-resolved genome assembly -- Phasing DNA Methylation -- Analysis of 1276 haplotype-resolved genomes allows characterization of cis- and trans-abundant genes -- Phased genome assemblies -- Haplotype-assisted noninvasive prenatal diagnosis of genetic diseases by massively parallel sequencing of maternal plasma cell-free DNA. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 24
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Bioinformatics. ; Pharmaceutical chemistry. ; Immunology. ; Bioinformatics. ; Pharmaceutics.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details state-of-the- art methods on computer-aided antibody design. Chapters guide readers through information on antibody sequences and structures, modeling antibody structures and dynamics, prediction and optimization of biological and biophysical properties of antibodies, prediction of antibody-antigen interactions, and computer-aided antibody affinity maturation and beyond. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computer-Aided Antibody Design aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. Chapter 2 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
    Type of Medium: Online Resource
    Pages: XIV, 491 p. 139 illus., 118 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626092
    Series Statement: Methods in Molecular Biology, 2552
    Language: English
    Note: Antibody sequence and structure analyses using IMGT®: 30 years of immunoinformatics -- Structural Classification of CDR-H3 in Single-Domain VHH Antibodies -- Computational modelling of Antibody and T Cell receptor (CDR3 loops) -- Molecular dynamics simulation for investigating antigen-antibody interaction -- Molecular Dynamics Methods for Antibody Design -- Probing antibody dynamics with geometric simulations -- PITHA: a webtool to predict immunogenicity for humanized and fully human therapeutic antibodies -- Thermal Stability Estimation of Single Domain Antibodies Using Molecular Dynamics Simulations -- Assessing and engineering antibody stability using experimental and computational methods -- In Silico Prediction Method for Protein Asparagine Deamidation -- Structure-based Optimization of Antibody-Based Biotherapeutics for Improved Developability: A Practical Guide for Molecular Modelers -- B-cell epitope predictions using computational methods -- Computational Epitope Prediction and Design for Antibody Development and Detection -- Information-driven antibody-antigen modelling with HADDOCK -- Structural modeling of adaptive immune responses to infection -- Protein-Protein Interaction Modelling with the Fragment Molecular Orbital Method -- Structural Considerations in Affinity Maturation of Antibody-Based Biotherapeutic Candidates -- Structure-based affinity maturation of antibody based on double-point mutations -- Antibody affinity maturation using computational methods: from an initial hit to small scale expression of optimised binders -- Optimizing Antibody-Antigen Binding Affinities with the ADAPT Platform -- Using graph-based signatures to guide rational antibody engineering -- A computational framework for determining the breadth of antibodies against highly mutable pathogens -- Analytical method for experimental validation of computer-designed antibody -- Computational analysis of antibody paratopes for antibody sequences in antibody libraries -- Bioinformatic Analysis of Natively Paired VH:VL Antibody Repertoires for Antibody Discovery -- Analyzing antibody repertoire using next-generation sequencing and machine learning -- A computational pipeline for predicting cancer neoepitopes. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 25
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Proteins. ; Cytology—Technique. ; Proteins. ; Cytological Techniques.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.
    Type of Medium: Online Resource
    Pages: XII, 286 p. 41 illus., 31 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071628638
    Series Statement: Methods in Molecular Biology, 2603
    Language: English
    Note: Dynamic SILAC to Determine Protein Turnover in Neurons and Glia -- SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe -- Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics -- pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles -- Quantification of Protein Palmitoylation by Cysteine-SILAC -- Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC) -- A Super-SILAC Approach for Profiling Histone Post-Translational Modifications -- SILAC-Based Quantitative Phosphoproteomics in Yeast -- Phosphotyrosine Profiling Using SILAC -- Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins -- Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules -- Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC -- Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons -- Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry -- SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos -- Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae -- Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC -- Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions -- Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC -- Using SILAC to Develop Quantitative Data Independent Acquisition (DIA) Proteomics Methods -- SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status -- Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-Length Protein Calibrators (TAQSI).
    Signatur Availability
    BibTip Others were also interested in ...
  • 26
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Proteins. ; Proteins.
    In: Springer Nature eBook
    Description / Table of Contents: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. .
    Type of Medium: Online Resource
    Pages: XII, 485 p. 82 illus., 74 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071628317
    Series Statement: Methods in Molecular Biology, 2596
    Language: English
    Note: Two-Dimensional Gel Electrophoresis and 2D-DIGE -- Top-down Proteomics and Comparative 2D-DIGE Analysis -- DIGE Analysis Software and Protein Identification Approaches -- Native DIGE for Quantitative and Functional Analysis of Protein Interactomes -- Comparative Two-dimensional Fluorescence Gel Electrophoresis -- Identification of Ubiquitination associated Proteins using 2D-DIGE -- DIGE-based Phosphoproteomic Analysis -- DIGE-based Biomarker Discovery in Blood Cancers -- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue -- DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples -- Comparative 3-sample 2D-DIGE Analysis of Skeletal Muscles -- Proteomic identification of saliva proteins as non-invasive diagnostic biomarkers -- Analysis of the saliva proteome using 2D-DIGE -- DIGE Analysis of Clinical Specimens -- DIGE Analysis of Animal Tissues -- Rapid 2D-DIGE Proteomic Analysis of Mouse Liver within One Week -- 2D-DIGE analysis of liver disease in mice -- 2-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis -- 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens -- Identification of Subproteomic Markers for Skeletal Muscle Profiling -- DIGE Analysis of Fish Tissues -- Sample preparation and protein determination for 2D-DIGE proteomics -- Protein Digestion for 2D-DIGE Analysis -- Subcellular Fractionation for DIGE-based Proteomics -- DIGE Analysis of Immunodepleted Plasma -- Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue -- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays -- Enzyme Assay Methods to Validate DIGE Proteomics Data -- Immunoblot Analysis of DIGE-based Proteomics -- Verification of Protein Changes determined by 2D-DIGE based Proteomics using Immunofluorescence Microscopy -- Histological and Histochemical Microscopy used to verify 2D-DIGE Pathoproteomics. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 27
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurosciences. ; Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume discusses the latest imaging and molecular techniques for studying microcephaly through neural progenitor proliferation, survival, and gene expression. All of the methods covered in this book use the mouse as a model system, and include cellular, metabolic, transcriptomic studies of neural progenitors, MR imaging studies of brain growth in 3-dimensions and developmentally-adapted studies of behavior in neonatal mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Microcephaly: Methods and Protocols is a valuable resource for all researchers who want to expand their knowledge and understanding of this important and developing field.
    Type of Medium: Online Resource
    Pages: X, 176 p. 36 illus., 24 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627525
    Series Statement: Methods in Molecular Biology, 2583
    Language: English
    Note: Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular Biology -- Maintaining Cerebellar Granule Neuron Progenitors in Cell Culture -- Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry -- Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitors -- Processing of Neonatal Mouse Brains for Immunohistochemical Analysis, As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis -- Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections -- Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections -- scRNA-seq for Microcephaly Research [1]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis -- scRNA-seq for Microcephaly Research [2]: Preparation of Single-Cell cDNA Libraries -- scRNA-seq for Microcephaly Research [3]: Computational Analysis of scRNA-seq Data -- scRNA-seq for Microcephaly Research [4]: Dirichlet Regression for Single Cell Population Differences -- Mouse Brain MRI, Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly -- Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly, using 13C Isotopes -- Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 28
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Botany. ; Plant Science.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. .
    Type of Medium: Online Resource
    Pages: XVI, 415 p. 80 illus., 57 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071627846
    Series Statement: Methods in Molecular Biology, 2581
    Language: English
    Note: Observing real-time ubiquitination in high throughput with fluorescence polarization -- Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating-Enzymes and E3 Ubiquitin Ligases -- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis thaliana seedlings -- An in vitro assay to recapitulate hormone-triggered & SCF-mediated protein ubiquitylation -- Analysis of proteasome-associated ubiquitin ligase activity -- Measuring the DUB Activity of Arabidopsis Deubiquitylating enzymes -- SUMO conjugation and SUMO chain formation by plant enzymes -- Kinetic analysis of plant SUMO conjugation machinery -- Expression, purification, and enzymatic analysis of plant SUMO proteases -- Monitoring autophagic flux in the model single-celled microalga Chlamydomonas reinhardtii -- Detection of autophagy in plants by fluorescence microscopy -- Characterization of ATG8-family interactors by Isothermal Titration Calorimetry -- Protocols for studying protein stability in a Arabidopsis protoplast transient expression system -- Relative Protein Lifetime Measurement in Plants using Tandem Fluorescent Protein Timers -- Detection and Quantification of Protein Aggregates In Plants -- Using intrinsic fluorescence to measure protein stability upon thermal and chemical denaturation -- Purification and detection of ubiquitinated plant proteins using Tandem Ubiquitin Binding Entities -- Titanium oxide-based phosphopeptide enrichment from Arabidopsis seedlings -- Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea -- Chromatin Enrichment for Proteomics in Plants (ChEP-P) -- Proximity-dependent in vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha -- Tandem mass tag-based phospho-proteomics in plants -- Analysis of peptide hormone maturation and processing specificity using isotope-labeled peptides -- Improved identification of protease cleavage sites by in-gel reductive dimethylation -- A Pipeline to Monitor Proteasome Homeostasis in Plants -- Bioinformatics tools for exploring the SUMO Gene Network, an update -- Coexpression network constriction and visualization from transcriptomes underlying ER stress responses -- Approaches for the identification of intrinsically disordered protein domains.
    Signatur Availability
    BibTip Others were also interested in ...
  • 29
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Analytical biochemistry. ; Pharmacology. ; Analytical Biochemistry. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
    Type of Medium: Online Resource
    Pages: XVII, 509 p. 138 illus., 65 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071627280
    Series Statement: Methods in Molecular Biology, 2576
    Language: English
    Note: Need for Methods to Investigate Endocannabinoid Signaling -- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues -- Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies -- LC-MS/MS Analysis of AEA and 2-AG -- Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices -- Endocannabinoid-Binding Receptors as Drug Targets -- Assay of CB1 Receptor Binding -- The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Fluorescence-based Assay for TRPV1 Channels -- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding -- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ and PPARδ Receptors -- A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγ -- The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Assay of GTPγS Binding in Autoradiography -- Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2 -- Endocannabinoid Metabolism and Transport as Drug Targets -- Assay of NAT Activity -- Radiometric Assay of NAPE-PLD activity -- A Fluorescence-based NAPE-PLD Activity Assay -- Radiometric Assay of FAAH Activity -- Fluorimetric Assay of FAAH activity -- Assay of NAAA Activity -- Assay of DAGLα/β Activity -- Assay of Monoacylglycerol Lipase Activity -- Radiometric Assay of ABHD2 Activity -- Oxygenation of Anandamide by Lipoxygenases -- Assay of Endocannabinoid Oxidation by Cytochrome P450 -- Assays of Endocannabinoid Uptake -- Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction -- Bioinformatics of the Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene promoter -- DNA Methylation Analysis of Cnr1 Gene Promoter -- Western Blotting of the Endocannabinoid System -- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells -- Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal and Electron Microscopy -- Measuring Endocannabinoid System Interaction with Biomembranes -- STORM Super-Resolution Imaging of CB1 Receptors in Tissue Preparations -- Visualization of Endocannabinoids in the Cell -- Electrophysiology of Endocannabinoid Signalling -- Machine Learning and Computational Chemistry for the Endocannabinoid System -- A Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor.
    Signatur Availability
    BibTip Others were also interested in ...
  • 30
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Analytical biochemistry. ; Immunology. ; Pharmacology. ; Analytical Biochemistry. ; Immunology. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.
    Type of Medium: Online Resource
    Pages: XII, 261 p. 63 illus., 59 illus. in color. , online resource.
    Edition: 3rd ed. 2023.
    ISBN: 9781071627327
    Series Statement: Methods in Molecular Biology, 2578
    Language: English
    Note: Applications of Peptide Microarrays in Autoantibody, Infection, and Cancer Detection -- Peptide Microarrays for Studying Autoantibodies in Neurological Disease -- A Bi-Functional Polymeric Coating for the Co-Immobilization of Proteins and Peptides on Microarrays Substrates -- Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry -- Hybrid Peptide-Agarose Hydrogels for 3D Immunoassays -- Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes -- Fast Protocols for Characterizing Antibody-Peptide Binding -- Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays -- One-Shot Generation of Epitope-Directed Monoclonal Antibodies to Multiple Non-Overlapping Targets: Peptide Selection, Antigen Preparation, and Epitope Mapping -- Peptide Microarray-Based Protein Interaction Studies Across Affinity Ranges: Enzyme Stalling, Cross-Linking, Depletion, and Neutralization -- Profiling of Multiple Matrix Metalloproteinases Activities in the Progression of Osteosarcoma by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Coated Zinc Oxide Nanorod Substrate -- Peptide Array-Based In Situ Fluorescence Assay for Profiling Multiple Matrix Metalloproteinases Activities -- Peptide-Based Sensor and Microfluidic Platform for IgG Antibody Detection by Differential Impedance Sensing -- Peptide Microarrays for Flavivirus Diagnosis -- Epitope Mapping on Microarrays, Highlight a Sequence on the N Protein with Strong Immune-Response in SARS-CoV-2 Patients -- IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay -- Extracellular Vesicles Molecular Profiling for Diagnostic Purposes: An Application of Phage-Display Technology -- Membrane-Sensing Peptides for Extracellular Vesicles Analysis.
    Signatur Availability
    BibTip Others were also interested in ...
  • 31
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurochemistry. ; Enzymology. ; Pharmaceutical chemistry. ; Neurochemistry. ; Enzymology. ; Pharmaceutics.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vital resource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.
    Type of Medium: Online Resource
    Pages: X, 254 p. 91 illus., 56 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626436
    Series Statement: Methods in Molecular Biology, 2558
    Language: English
    Note: Purification of MAO A and of MAO B from Mammalian Tissue Sources -- Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System -- The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity -- Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays -- Radiochemical Assay of Monoamine Oxidase Activity -- MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition -- Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase -- Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS) -- Crystallization of Human Monoamine Oxidase B -- Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations -- An (Immune)Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution within the Cell -- Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene-Transfer -- In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers -- In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis -- Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors -- Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology -- Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors.
    Signatur Availability
    BibTip Others were also interested in ...
  • 32
    Subject(s): Zoology. ; Animal culture. ; Invertebrates. ; Zoology. ; Animal Science. ; Invertebrate Zoology.
    In: Springer Nature eBook
    Description / Table of Contents: This authored book is primarily for the readers who are in search of how to use basic concepts of statistics in the field of fishery science. It has been written for the new comers coming in the fishery sector including university students, teachers and research scholars. It enriches the reader’s knowledge of data analysis step by step starting from data collection to its analysis and interpretation along with practical application with real example. It supports readers for enhancing theoretical and practical concept of application of fundamental statistics in fishery domain. Reader may also learn how to analyses data using different statistical software. Each chapter starts with an introduction of the topic followed by statistical concept with example and ends with chapter based question for comprehension purpose. This book is of interest to teachers, researchers, scientists, capacity builders in fishery sciences. Also the book serves as additional reading material for undergraduate and graduate students of fisheries and aquatic sciences.
    Type of Medium: Online Resource
    Pages: XXVIII, 257 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9789811944116
    Language: English
    Note: Chapter 1. Nature And Sources of Data For Inland Fisheries Management -- Chapter 2. Concept of Sampling Methodologies and Their Applications -- Chapter 3. Descriptive Statistics for Inland Fisheries Data -- Chapter 4. Basic Concept of Hypothesis Testing and Parametric Test -- Chapter 5. Chi–Square Test of Significance -- Chapter 6. Use of Non–Parametric Test in Fisheries Data -- Chapter 7. Analysis of Variance (Anova) and Design of Experiments -- Chapter 8. Concept of Simple Correlation and Regression -- Chapter 9. Analysis and Interpretation of Weight–Length Data of Fish -- Chapter 10. Some Multivariate Analysis Techniques for Fisheries Data -- Chapter 11. Introduction to Electronic Spreadsheet and Microsoft Excel -- Chapter 12. Introduction to R Software -- Chapter 13. Introduction To Graphics in R.
    Signatur Availability
    BibTip Others were also interested in ...
  • 33
    Subject(s): Toxicology. ; Pharmaceutical chemistry. ; Drug abuse. ; Criminology. ; Toxicology. ; Pharmaceutics. ; Drugs.
    In: Springer Nature eBook
    Description / Table of Contents: This collection provides detailed information on current advances in analytical methods and strategies employed for monitoring and discovering a wide range of novel psychoactive substances (NPS) in clinical and forensic laboratories. The main classes of NPS in terms of prevalence include synthetic cannabinoids, synthetic cathinones, synthetic opioids, and designer or synthetic benzodiazepines, and this book explores selecting the appropriate sample matrix and analytical testing approaches for laboratories faced with NPS drug testing, such as in blood, urine, saliva, and hair. Written for the Methods in Pharmacology and Toxicology series, chapters in this volume feature the kind of detailed implementation advice from the experts that leads to successful results in the lab. Authoritative and practical, Methods for Novel Psychoactive Substance Analysis serves as an ideal guide for forensic and clinical toxicologists, pharmacologists and chemists in academic and research settings, as well as for private laboratories seeking to increase our ability to test for these substances. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}@font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-469750017 -1073732485 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; mso-bidi-font-size:11.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-bidi-font-size:11.0pt; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}.
    Type of Medium: Online Resource
    Pages: X, 280 p. 27 illus., 10 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626054
    Series Statement: Methods in Pharmacology and Toxicology,
    Language: English
    Note: Introduction/Overview -- Target analysis of synthetic cannabinoids in blood and urine -- Target analysis of synthetic cathinones in blood and urine -- Target analysis of phenethylamines, piperazines and tryptamines in blood and urine -- Target analysis of synthetic opioids in blood and urine -- Target analysis of synthetic benzodiazepines in blood and urine -- Target analysis of NPS in alternative matrix hair -- Target analysis of NPS in alternative matrix oral fluid -- Target analysis of NPS in other alternative matrices (dried blood spots, meconium) -- Target analysis of NPS in postmortem samples -- High Resolution Mass Spectrometry in NPS qualitative analysis -- High Resolution Mass Spectrometry in NPS quantitative analysis -- High Resolution Mass Spectrometry in identification of new analytes & metabolites -- Alternative NPS screening approaches for high-throughput laboratories.
    Signatur Availability
    BibTip Others were also interested in ...
  • 34
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Biotic communities. ; Food science. ; Biodiversity. ; Environmental sciences—Social aspects. ; Ecosystems. ; Food Science. ; Biodiversity. ; Environmental Social Sciences.
    In: Springer Nature eBook
    Description / Table of Contents: In recent decades, practices like the cultivation of a few high-yielding crop varieties on a large scale, the application of heavy machinery and continued mechanization of agriculture, the removal of natural habitats, and the application of pesticides and synthetics have resulted in the simplification of agro-ecosystems. This has enabled a substantial increase in food production but has at the same time transformed landscapes. Indeed, there is a concern that a decline in biodiversity has affected microbiome activities that support processes across soils, plants, animals, the marine environment, and humans. Although they have increased food production, the above practices cannot be considered sustainable in long-term applications. Biodiversity, Functional Ecosystems, and Sustainable Food Production explore ecosystems in terms of crop and animal production, pest and disease control, nutrient cycling, and soil fertility. Chapters range from agro-biodiversity to antimicrobial use in animal food production to microbiome applications for sustainable food systems and the impacts of environment-friendly unit operations on the functional properties of bee pollen. By examining such topics about each other, the text emphasizes how food production, ecosystem function, food quality, and consumer health are all interconnected.
    Type of Medium: Online Resource
    Pages: X, 354 p. 90 illus., 40 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031074349
    Language: English
    Note: 1. Agro-biodiversity across the food chain -- 2. Emerging risks to plant health -- 3. Future-proofing Plants against Climate Change- A path to ensure Sustainable Food Systems -- 4. The role of Integrated Pest Management for sustainable food production: The soybean example -- 5. (Alternative approaches to pesticide use): Plant-derived pesticides -- 6. Antimicrobial use in animal food production -- 7. Impacts of environment-friendly unit operations on the functional properties of bee pollen -- 8. Microbiome applications for sustainable food systems -- 9. Healthier and sustainable food systems: integrating underutilized crops in a ‘Theory of Change Approach -- 10. Alternative proteins for food and feed.
    Signatur Availability
    BibTip Others were also interested in ...
  • 35
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Neurosciences. ; Zoology. ; Neurochemistry. ; Neuroscience. ; Zoology. ; Neurochemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This new edition provides comprehensive coverage of the variety and complexity of the roles that glycoconjugates play in the cells of the nervous system. Basic fundamental principles as well as the latest developments in neural glycobiology are discussed. Topics covered range from the structure and metabolism of the saccharide chains and current approaches used in their study, to changes glycoconjugates undergo during development and aging of the nervous system and the roles they have in neurological disease. New topics include a detailed discussion of cells found within the nervous system, an extensive listing of congenital disorders of glycosylation of both proteins and lipids, the roles of glycans in neuronal axon growth/guidance and voltage-gated channels, the role of intra-lysosomal luminal vesicles in lysosomal storage disorders, and, in the time of the COVID-19 pandemic, the role of carbohydrates in infection by SARS-CoV-2. The breadth and depth of topics covered make this an essential reference for those new to the field as well as for more experienced investigators.
    Type of Medium: Online Resource
    Pages: XII, 501 p. 96 illus., 73 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9783031123900
    Series Statement: Advances in Neurobiology, 29
    Language: English
    Note: 1. Introduction to the complexity of cell surface and tissue matrix glycoconjugates. -- 2. Introduction to cells comprising the nervous system. -- 3. Synthesis -- processing -- and function of N-glycans in N-glycoproteins. -- 4. Synthesis of O-linked glycoconjugates in the nervous system. -- 5. Chemistry and function of glycosaminoglycans in the nervous system. -- 6. Use of glycan-targeted antibodies/lectins to study the expression/function of glycosyltransferases in the nervous system. -- 7. Capillary electrophoresis and mass spectrometry-based glycosylation analysis in glycomics and glycoproteomics. -- 8. Structural analysis of oligosaccharides and glycoconjugates using NMR. -- 9. Glycolipid and glycoprotein expression during neural development. -- 10. Gangliosides and cell surface ganglioside glycohydrolases in the nervous system (request them to include gal-cer and sulfatide). -- 11. Role of myelin-associated glycoproteins in the peripheral nervous system. -- 12. Glycosignaling: a general review. -- 13. Glycosphingolipids in the regulation of the nervous system. -- 14. O-GlcNAcylation of neuronal proteins: Roles in neuronal functions and in neurodegeneration. -- 15. Glycoconjugates in nuclear membranes. -- 16. N-glycosylation in regulation of the nervous system. -- 17. Role of carbohydrates in the interaction of pathogens with neural cells. -- 18. Glycoconjugate changes in aging and age-related diseases. -- 19. Gangliosides and glycolipids in neurodegenerative disorders. -- 20. Inborn errors of glycosphingolipid catabolism and potential treatment. -- 21. Neurological aspects of human glycosylation disorders. -- 22. Glycoconjugates and neural tumors. -- 23. Galectins and neuroinflammation . -- 24. Glycoconjugates and neuroimmunological diseases. -- 25. Targeting nanoparticle drug carriers to the brain. -- 26. Use of quantum dots to treat inherited lysosomal storage diseases. -- 27. MALDI-FTICR MS imaging of nervous system glycoconjugates.
    Signatur Availability
    BibTip Others were also interested in ...
  • 36
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurosciences. ; Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation; visualization of 3D organoids though the latest advancements in microscopy; generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors; modeling brain tumors using genetically edited brain organoids; and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.
    Type of Medium: Online Resource
    Pages: XV, 222 p. 47 illus., 44 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071627204
    Series Statement: Neuromethods, 189
    Language: English
    Note: Self-Organizing Brain Organoids with Ventricles Amenable to Injection and Electroporation -- Profiling Cell Type-Specific Gene Regulatory Regions in Human Cortical Organoids -- Visualization of 3D Organoids Through the Latest Advancements in Microscopy -- Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting -- Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice -- Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells -- Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids -- A Three-Dimensional Organoid Culture System to Model Invasive Patterns of Patient-Derived Glioma Stem Cells -- Modeling Brain Tumors using Genetically Edited Brain Organoids -- Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 Infections -- Infection of 3D Brain Organoids with Human Pathogenic Viruses Under Biosafety Level 3 Conditions with Subsequent Inactivation to Study Viral Replication, Pathomechanisms, and Other Viral Infection-Mediated Effects.
    Signatur Availability
    BibTip Others were also interested in ...
  • 37
    Subject(s): Medicinal chemistry. ; Botany. ; Pharmacology. ; Medicinal Chemistry. ; Plant Science. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume explores a wide range of evidence-based complementary medicine and various bio-analytical techniques used to define botanical products. Collecting recent work and current developments in the field of contemporary phytomedicine as well as their future possibilities in human health care, the book includes unique contributions in the form of chapters on phytomedicine and screening biological activities explained with diverse hyphenated techniques, as well as issues related to herbal medications, such as efficacy, adulteration, safety, toxicity, regulations, and drug delivery. Written for the Springer Protocols Handbooks series, chapters feature advice from experts on how to best conduct future experiments. Extensive and practical, Natural Product Experiments in Drug Discovery serves as an ideal reference for students, professors, and researchers in universities, R&D institutes, pharmaceutical and herbal enterprises, and health organizations.
    Type of Medium: Online Resource
    Pages: XVIII, 561 p. 157 illus., 126 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626832
    Series Statement: Springer Protocols Handbooks,
    Language: English
    Note: Phytochemical, Antioxidant, and Flavonoid Investigation of Methanolic Leaf Extract of Piliostigma Thoningii (Schum.) -- A Comparative Evaluation of Organoleptic, Physicochemical, Proximate, and Mineral Composition of Syzygium laetum and Syzygium occidentale, Endemic to Western Ghats: A Search for Low-Cost Nutraceuticals -- Chemical Characterization, Biological Activities, and Some Medicinal Uses of Different Sweet Basil (Ocimum basilicum L.) Genotypes -- Estimation of Primary, Secondary Metabolites, and In Vitro Free Radical Scavenging Activities with Ficus tsjahela Burm. F. Crude Extracts -- Evaluating the Lipid Profile and Mineral Composition of the Seed Oil of Caesalpinia sappan L. (Caesalpiniaceae) -- Phytochemical Screening, Physicochemical Analysis, and Powder Characterization of Selected Litsea Species Endemic to the Southern Western Ghats -- Metabolite Profiling of Bioactive Compounds in Selected Species of Gomphostemma Wall. ex Benth. from the Western Ghats by GC-MS Analysis -- Phytochemistry and Pharmacology of Aromatic Medicinal Plant Commiphora caudata(Wight &Arn.)Engl. -- Unravelling the Phytochemistry of Ethnomedicinal Tree Pajanelia longifolia (Wild.) K. Schum. -- Comparative Phycochemical Investigation and Antioxidant Content of Two Marine Seaweeds -- Evaluation of Nutritional, Antioxidant and Anti-Arthritic Activity of Hedychium coronarium J. Koenig Rhizome -- Phytochemical, Pharmacognostic, and In Vitro Acetylcholinesterase Inhibitory Activities of Cipadesa baccifera Leaves -- Comparative Study of Drug Efficiency of Scemecarpus anacardium and Tridax procumbens against Zika Virus -- Evaluation of In Vitro Antioxidant Activity of Oldenlandia dineshii: An Endemic Plant from the Hillocks of Palakkad District, Kerala, India -- Identification of Secondary Metabolites through HR-GC-MS: Antibacterial and Antifungal Activity from Root Fractions of Alstonia scholaris (L.) RBr. from Andhra Pradesh, South India -- In Vitro Models on Inhibition of Inflammatory Mediators by Ethanolic Extract of Careya arborea Roxb. Bark in Lipopolysaccharide-Induced RAW 264.7 Cell Lines -- Pharmacological Elucidation of Antioxidant, Hypoglycemic, and Anti-Inflammatory Potentials of Phyllanthus candolleanus (Wights ARN) Chakrab. -- α-Glucosidase Inhibition and Upregulation of PPARγ by Flavonoid Naringenin from Tinospora sinensis Stem, a Possible Mechanism of Antidiabetic Activity -- Purification and Characterization of a Cysteine Protease from Sprouted Lablab purpureus Seed Radicle Extract: Its Effect on Blood Coagulation -- Effect of Sterilization Agents, Growth Regulators, and Activated Charcoal on Callus Cultures of Evolvulus alsinoides (Linn.) Linn -- Bryophytes: A Myriad Hue of Bio-Resources with Therapeutic Potentialities -- Recent Biotechnological Approaches for the Enhancement of Artemisinin Production from Cell Culture System of Artemisia annua L: Medicinal Plant with Potent Anti-Malarial Properties -- The Utility of Natural Mucilage from the Medicinal Plant, ‘Patha’ (Cyclea peltata), as an Alternative for Solidifying Agent in Cell Growth Media -- Microwave Irradiation-Assisted Extraction and Synthesis of Silver Nanoparticles Using Tree Tomato Fruit Pulp (Solanum betaceum Sendt.) and Determination of In Vitro Cytotoxic Effect on MCF-7 Cells -- Characterization of Crystals Isolated from the Caesalpinia sappan Seeds: A Comparative In Silico Analysis on Bioactivity of Sappan Diterpenoids -- Computational Analysis of Natural Compounds as Potential Isocitrate Dehydrogenase 1 Inhibitor for the Treatment of Ovarian Cancer -- Drug Efficiency of Oroxylum Indicum and Taxus Wallichiana Against Colon-Associated Diseases: In Silico Studies -- Gas Chromatography-Mass Spectrometry Analysis of Phytoconstituents from Garcinia gummi-gutta Seed Extract: Trypsin Inhibition Study of Extract and Molecular Docking Study -- In Silico Analysis of Anti-Inflammatory Activity of Quercetin from M. Calabura Fruit -- Phytochemical Investigation, Molecular Docking Studies, and Biological Efficacy of Rhizome Essential Oil Composition of Costus Speciosus -- Ficus talboti King: Anti-Nociceptive and Anti-Inflammatory Properties of Methanol Extract of Stem Bark -- Phytochemical and Antioxidant Analysis of Phellinus adamantinus and P. hohnelli.
    Signatur Availability
    BibTip Others were also interested in ...
  • 38
    Online Resource
    Online Resource
    Singapore : Springer Nature Singapore
    Subject(s): Microbiology. ; Biology—Technique. ; Biotechnology. ; Microbiology. ; Biological Techniques. ; Biotechnology.
    In: Springer Nature eBook
    Description / Table of Contents: This edited book presents all feasible approaches to improve technology of algal biofuels production at both qualitative and quantitative front. The book’s focus in on enhancing mass scale production of algae based biofuels by addressing technological issues and filling the existing gaps to make it smooth for practical as well as commercial implementation. The book also explores in depth analysis of various issues other than technology and related to improve technological significance for practical biofuels production from algae. Low cost strategies and higher mass production is one of the most sounding agenda of the book. The book also evaluates enlighten various sustainable algal biofuels options which are close towards commercial application along with their green future prospect. Societal and environment friendly approach even for commercial application has also been discussed in book. This is a useful reading material for researchers and students of biofuels and reneable energy. .
    Type of Medium: Online Resource
    Pages: XI, 295 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9789811968068
    Series Statement: Clean Energy Production Technologies,
    Language: English
    Note: Chapter 1. Biotechnological approaches to enhance algae biofuel production -- Chapter 2. The use of omics technologies, random mutagenesis, and genetic transformation techniques to improve algae for biodiesel industry -- Chapter 3. Algal butanol production: recent developments -- Chapter 4. Algal synthesis of gold nanoparticles: applications in bioenergy -- Chapter 5. Challenges assessment in economic Algal biofuel Production -- Chapter 6. Influence of culture conditions on the microalgae biomass and lipid accumulation -- Chapter 7. Advanced genetic approaches towards custom design microalgae for fourth-generation biofuels -- Chapter 8. Algal biofuel production from municipal waste waters -- Chapter 9. Positive influence and future perspective of marine alga on biofuel production -- Chapter 10. Algae bacterial mixed culture for waste to wealth conversation: a case study.
    Signatur Availability
    BibTip Others were also interested in ...
  • 39
    Subject(s): Ecology . ; Biotic communities. ; Biodiversity. ; Freshwater ecology. ; Marine ecology. ; Ecology. ; Ecosystems. ; Biodiversity. ; Freshwater and Marine Ecology.
    In: Springer Nature eBook
    Description / Table of Contents: This edited book brings out a comprehensive account of the faunal diversity in Great Nicobar Biosphere Reserve which is part of the Sundaland Biodiversity hotspot. The main focus of this volume is to address the ecological and economic significance of the biodiversity of this region. The book provides latest information on the faunal diversity of Great Nicobar Biosphere. It focuses on various faunal communities of this regions such as Floral diversity, Butterflies, Moths, Dragonflies & Damselflies, Termites, Hymenoptera, Coleoptera, Scorpion, Spiders, Turtle, Nicobar megapode. Chapters in this volume also covers Reptiles & Amphibians, Fishes, Mammals, Land and freshwater Mollusca. Marine animals such as Opisthobranchs, Sponges, Scleractinian Corals and Soft corals, Sea Anemones, Polyclads, Marine Molluscs, Echinoderms are also discussed. It is of interest for the researchers, teachers, scientists, capacity builders, policy makers. Also serve as reading materials for graduate and post graduate students e.g., Wildlife Biology, Forestry, Environmental Science, Wildlife Ecology, Zoology, Life sciences, Parks/Sanctuaries managers, National and International scientists, policy makers will also find this to be a useful document.
    Type of Medium: Online Resource
    Pages: XVI, 666 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9789811951589
    Language: English
    Note: 1. Great Nicobar Biospehere reserve: An Overview -- 2. Floral diversity of great nicobar biospehere reserve Andaman & Nicobar Islands, INDIA -- 3. Odonata of great nicobar biosphere reserve -- 4. Butterfly diversity of great nicobar biospehere reserve -- 5. Aquatic beetles of great nicobar biosphere reserve - (Coleoptera: Noteridae, Dytiscidae, and Hydrophildae) -- 6. Moths (Lepidoptera) of great nicobar biosphere reserve -- 7. Termite fauna of great nicobar biosphere reserve, India -- 8. Scorpionida and amblypygi diversity of great nicobar biosphere reseve -- 9. Diversity and species abundance of spiders of great nicobar biosphere reserve -- 10. Non-Marine Molluscs of great nicobar biosphere reserve, andaman and nicobar island, India -- 11. Reptiles of great nicobar biosphere reserve -- 12. Fresh water and estuarine fishes of great nicobar islands -- 13. Diversity and species abundance of bird commnuities in great nicobar biosphere reserve, India -- 14. Present status and distribution of nicobar megapode megapodius nicobariensis in nicobar islands -- 15. Mammalian fauna of great nicobar biosphere reserve -- 16. Status and distribution of long-tailed macaque fascicularis umbrosa miler, 1902 in great nicobar biosphere reserve, India -- 17. Conservation perspectives of dugongs and sea turtles in andaman and nicobar islands -- 18. Mesozooplankton diversity in the marine waters of great nicobar island, andaman and Nicobar Archipelago -- 19. Current status of diversity of marine microplankton from great nicobar island, andaman and nicobar archipelago -- 20. Marine sponges of great nicobar biosphere reserve -- 21. Great Nicobar Biosphere reserve - A Biological treasure trove for scleractinan corals -- 22.Hydrozoan diversity and distribution in great nicobar biosphere reserve -- 23. Diversity and distribution of octocorals (Octocorollia) in great nicobar biosphere reserve -- 24. Status and distribution of sea anemone in great nicobar biosphere reserve -- 25. Polyclads of great nicobar biosphere reserve -- 26. Decapods (Crustacea: Decapoda) of great status of great Nicobar biosphere reserve -- 27. Current status of the marine polychaete (Annelida: Polychaeta) from Nicobar islands -- 28. Diversity and distribution of intertdial mollusca of great nicobar biosphere reserve. 29. Status and distribution of Opisthobranchs of Great nicobar isalnd, India -- 30. Diversity and distribution of echinoderms of great niocobar biosphere reserve, Andaman and Nicobar islands -- 31. Marine Icthyofaunal Diversity in great Nicobar Biosphere resere,- India. 32. Marine Mammals of the Nicobar group of Islands, India.
    Signatur Availability
    BibTip Others were also interested in ...
  • 40
    Subject(s): Neurosciences. ; Cognitive neuroscience. ; Psychobiology. ; Human behavior. ; Sociology. ; Neuroscience. ; Cognitive Neuroscience. ; Behavioral Neuroscience. ; Sociology.
    In: Springer Nature eBook
    Description / Table of Contents: This Open Access book presents the current state of the art knowledge on social and affective neuroscience based on empirical findings. This volume is divided into several sections first guiding the reader through important theoretical topics within affective neuroscience, social neuroscience and moral emotions, and clinical neuroscience. Each chapter addresses everyday social interactions and various aspects of social interactions from a different angle taking the reader on a diverse journey. The last section of the book is of methodological nature. Basic information is presented for the reader to learn about common methodologies used in neuroscience alongside advanced input to deepen the understanding and usability of these methods in social and affective neuroscience for more experienced readers.
    Type of Medium: Online Resource
    Pages: XXXIII, 302 p. 55 illus., 48 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9783031086519
    Language: English
    Note: Molecular imaging of the human emotion circuit -- The neurocognitive mechanisms of unconscious emotional responses -- Social and Affective Neuroscience of Embodiment -- The neuroscience of beauty -- Mirror Neurons … in action: ERPs and neuroimaging evidence -- Sex differences in social cognition -- Development of morality and emotional processing -- Trust in Social Interaction: From Dyads to Civilizations -- The time has come to be mindwanderful: Mind wandering and the intuitive psychology mode -- Social Cognition development and socio affective dysfunction in childhood and adolescence -- Clinical neuroscience meets second-person neuropsychiatry -- EEG and ERPs in the study of Language and Social Knowledge -- fMRI and fNIRS methods for social brain studies: hyperscanning possibilities -- Modulating the Social and Affective Brain with transcranial stimulation techniques -- What our eyes can tell us about our social and affective brain? -- Facial EMG – Investigating the interplay of facial muscles and emotions.
    Signatur Availability
    BibTip Others were also interested in ...
  • 41
    Subject(s): Biotic communities. ; Freshwater ecology. ; Marine ecology. ; Ecosystems. ; Freshwater and Marine Ecology.
    In: Springer Nature eBook
    Description / Table of Contents: This edited book covers all aspects of River related disasters, challenges, and opportunities. Step-by-step descriptions are provided of river dynamics and associated hazards, and their applications in hazard assessments, accompanied by several experimental, filed and numerical studies. In addition, a systematic table of content is given to aid in identifying River hazards challenges and opportunities. Essential information is provided on River dynamics, hydrological processes and climate change issues, and an individual chapter is devoted to ecological restoration and river hazard management. Further topics include the stability of hydraulic structures, sediment transport, and debris flow in the hilly streams. This book will provide students, researchers, scientists, water resources managers with a comprehensive overview of the River dynamics and flood hazards in various sectors of water-related disasters and will enable them to explore the scope of application of the computational techniques and will enable them to explore the scope of River related disasters, allied branches and their field-specific problems. Professionals and policymakers may also explore the implementation of these approaches in their workplace to tackle complex river dynamics and hydrological phenomena occurring in their study area.
    Type of Medium: Online Resource
    Pages: X, 624 p. 1 illus. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9789811971006
    Series Statement: Disaster Resilience and Green Growth,
    Language: English
    Note: Part (I) Introduction to river hazards and their management: Chapter 1. Natural River Hazards: Their Impacts and Mitigation Techniques -- Chapter 2. Assessment of sediment hazard and associated measurement -- Chapter 3. Modelling approach to study the riverine flood hazard of lower Damodar river -- Chapter 4. Field measurement of accumulated surface water and infiltration depth in a flood-prone Langol catchment of Manipur valley region -- Chapter 5. Soil erosion analysis with respect to land use / land cover change in Godavari basin -- Part (II) Stability of Hydraulic Structures and Sediment Transport: Chapter 6. Stability of hydraulic structures against erosion and scour due to water jets -- Chapter 7. Stabilization of Manmade Embankments at Indian Sundarban Estuary through Turbulence Control at Flow-Sediment Interface: Field Survey and Flume Experimentation -- Chapter 8. A Review of Sedimentation on Different Types of Weirs -- Chapter 9. A Review on Parametric Studies of Piano Key Weir -- Chapter 10 Influence of boundary condition on the modified 2D shallow water model near the flow-structure interaction zone: a case study in Brahmaputra River -- Chapter 11. A Review on Estimation Methods of Scour Depth around Bridge Pier -- Chapter 12. Estimation of Shear force Distribution in Two-stage open channel using SVM and ANFIS -- Chapter 13. Sediment transport modelling through machine learning methods: Review of current challenges and strategies -- Chapter 14. Impact of Anthropocene on the fluvial sediment supply: The Mahanadi River basin perspective -- Chapter 15. Assessment of sediment hazards by bed level variations around the bridge pier -- Chapter 16. Equation development for equilibrium bed load -- Part (III) Hydrological hazards and ecological restoration: Chapter 17. Flood mitigation with river restoration using Porcupine systems -- Chapter 18. Flood prioritisation of basins based on geomorphometric properties using Morphometric analysis and Principal component analysis: A case study of the Maner River basin -- Chapter 19. Flood modelling of Krishna river at Sangli using HEC-RAS -- Chapter 20. Development of Machine Learning based Flood Prediction Model for Godavari River Basin -- Chapter 21. Field study on soil organic matter content in inundation areas of Langol catchment by loss- on-ignition” method -- Chapter 22. Agricultural Drought Assessment using Satellite based Surface Soil Moisture Estimate -- Chapter 23. A Review on Hydrodynamics of Vegetated Streams -- Chapter 24. Analysis of Stormwater Drainage Network of the Central Zone in the Surat City by Using SWMM 5.1 Software -- Chapter 25. Review of state-of-the-art research on river hydrological hazards, restoration and management -- Chapter 26. Erosion Susceptibility Mapping based on Hypsometric Analysis using Remote Sensing and Geographical Information System Techniques -- Part (IV) Climate change and Global warming issues: Chapter 27. Climate Change Implication and Adaptation for River systems -- Chapter 28. Non-parametric approaches to identify rainfall pattern in semi-arid regions: Ranipet, Vellore, and Tirupathur districts, Tamil Nadu, India -- Chapter 29. Decadal-based analysis of hydrological components in the Kesinga sub-catchment in Mahanadi Basin: An assessment of climate variability impact -- Chapter 30. Prediction of Future Rainfall in the Upper Godavari Basin Using Statistical Downscaling Model -- Chapter 31. Projecting Future Maximum Temperature Changes in River Ganges Basin using Observations and Statistical Downscaling Model (SDSM) -- Chapter 32. Trend Assessment of Rainfall over Mumbai and Pune Cities -- Chapter 33. Evaluation of Potential lakes Susceptible to GLOF using Multi-Criteria Assessment in Jhelum Sub-basin of Indus Basin.
    Signatur Availability
    BibTip Others were also interested in ...
  • 42
    Subject(s): Microbiology. ; Enzymology. ; Virology. ; Microbiology. ; Enzymology. ; Virology.
    In: Springer Nature eBook
    Description / Table of Contents: This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
    Type of Medium: Online Resource
    Pages: XIII, 300 p. 62 illus., 49 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071628034
    Series Statement: Methods in Molecular Biology, 2591
    Language: English
    Note: Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach -- Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs -- Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell -- Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue -- Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases -- High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity -- Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels -- High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery -- Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases -- Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay -- Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease -- Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases -- Identification of Deubiquitinase Substrates in Xenopus Egg Extract -- Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression -- Real-time Deubiquitination Assays using a Free-Ubiquitin Sensor -- A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells -- Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast.
    Signatur Availability
    BibTip Others were also interested in ...
  • 43
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Cancer. ; Immunology. ; Cancers.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.
    Type of Medium: Online Resource
    Pages: XI, 384 p. 76 illus., 41 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071627402
    Series Statement: Methods in Molecular Biology, 2580
    Language: English
    Note: A beginner’s guide to T cell development -- Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies -- Early development of innate lymphoid cells -- Development of gd T cells, Soldiers on the Front Lines of Immune Battles -- Principles of Advanced Flow Cytometry: A Practical Guide -- Genetic strategies to study T cell development -- A simplified approach to evaluating T cell development by flow cytometry -- Purification of thymocyte and T cell subsets -- Generation of bone marrow chimeras -- Generation of murine T cell effector populations in vitro -- Large-scale isolation of mouse thymic epithelial cells -- Generation of retrogenic mice to investigate T cell developmentPurification of bone marrow precursors to T cells and ILCs -- Studying T cell Development in Neonatal and Adult Thymic Slices -- Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines -- Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements -- Ultrasound guided intra-thymic cell injection -- Fetal thymic organ culture and negative selection -- In Vitro Analysis of Thymocyte Signaling -- Identification and purification of human T cell precursors -- In vitro model systems to study human T cell development -- Zebrafish: A Tractable Model for Analysis of T cell Development. .
    Signatur Availability
    BibTip Others were also interested in ...
  • 44
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurosciences. ; Nervous system—Diseases. ; Neurophysiology. ; Neurons. ; Neuroscience. ; Neurological Disorders. ; Cellular Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores the latest techniques used to study the human brain towards understanding Alzheimer’s Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural cellular models using primary and human induced pluripotent stem cells to model aspects of the human brain. Part Three details nucleic acid analyses including transcriptomic and somatic genomic changes, and Part Four discusses lipid analyses via mass spectrometry. Lastly, Part Five covers protein analyses, particularly Aβ and Tau. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alzheimer’s Disease: Methods and Protocols is a valuable tool for all researchers who want to expand their knowledge and understanding of this disease and other related dementias.
    Type of Medium: Online Resource
    Pages: XIII, 340 p. 61 illus., 59 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626559
    Series Statement: Methods in Molecular Biology, 2561
    Language: English
    Note: Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue -- Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue -- Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era -- Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images -- Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer’s Disease -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease -- Modeling Alzheimer’s Disease using Human Brain Organoids -- 3D Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer’s Disease Pathology -- Computational Approaches to Assess Abnormal Metabolism in Alzheimer’s Disease Using Transcriptomics -- FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain -- Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Post-Mortem Human Brain -- Mass Spectrometry Analysis of the Human Brain Sphingolipidome -- Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research -- The Use of Affinity Purification-Mass Spectrometry to identify Phosphorylates Tau Interactors in Alzheimer’s Disease -- In Vitro Amplification of Pathogenic Tau Seeds from Neurodegenerative Disease Patient Brains -- Aβ and Tau Prions Causing Alzheimer’s Disease.
    Signatur Availability
    BibTip Others were also interested in ...
  • 45
    Subject(s): Mouth. ; Anatomy. ; Medicine—Research. ; Biology—Research. ; Oral Anatomy. ; Biomedical Research.
    In: Springer Nature eBook
    Description / Table of Contents: This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.