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  • Springer  (117,755)
  • Blackwell Publishing Ltd  (15,605)
  • 2010-2014  (2,549)
  • 1970-1974  (130,811)
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  • 1
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 2
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 3
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 4
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    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 5
    Keywords: Life sciences ; Microbiology ; Bacteriology ; Life sciences ; Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Methods for Dynamic Investigations of Surface Attached in vitro Bacterial and Fungal Biofilms -- Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms -- Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and enterococcal fsr Systems -- Advanced Techniques for in situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy -- Multiplex Fluorescence in situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyse Multispecies Oral Biofilms -- Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections -- Experimental Approaches to Investigating the Vaginal Biofilm Microbiome -- Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopaedic Infections -- Animal Models to Evaluate Bacterial Biofilm Development -- Animal Models to Investigate Fungal Biofilm Formation -- Non-Mammalian Model Systems to Investigate Fungal Biofilms -- Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors -- In vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development -- Biofilm Matrix-Degrading Enzymes -- Efficacy Evaluation of℗ Antimicrobial Drug-Releasing Polymer Matrices -- Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy -- Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa in vitro and in vivo -- Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity -- Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics and Biological Agents -- Bacteriophage Attack as an Anti-Biofilm Strategy -- Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study Protocols -- Capturing Air-Water Interface Biofilms for Microscopy and Molecular Analysis -- Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic Analyses -- Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Cultural Heritage Stone Materials and Frescoes -- Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants and Efficacy of Sanitizers
    Abstract: The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms
    Pages: XIII, 380 p. 100 illus., 62 illus. in color. : online resource.
    ISBN: 9781493904679
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  • 6
    Keywords: Medicine ; Oncology ; Vaccines ; Biomedicine ; Cancer Research ; Oncology ; Vaccine ; Springer eBooks
    Description / Table of Contents: Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules -- Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine -- Pulsing Dendritic Cells with Whole Tumor Cell Lysates -- Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy -- Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens -- Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma -- Dendritic Cells Primed with Protein-Protein Fusion Adjuvant -- Antigen-Specific mRNA Transfection of Autologous Dendritic Cells -- Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material -- Dendritic Cells Transfected with Adenoviral Vectors as Vaccines -- Genetic Modification of Dendritic Cells with RNAi -- Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy -- Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation -- Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines -- Modification of T Lymphocytes to Express Tumor Antigens -- Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor -- Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors -- Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients -- Cytokine Activation of Natural Killer Cells -- Loading of Acute Myeloid Leukemia Cells with Poly(I:C) by Electroporation -- Autologous Tumor Cells Engineered to Express Bacterial Antigens -- Tumor Cell Transformation Using Antisense Oligonucleotide -- The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy -- Cloning Variable Region Genes of Clonal Lymphoma Immunoglobulin for Generating Patient-Specific Idiotype DNA Vaccine -- Heat Shock Proteins Purified from Autologous Tumors Using Antibody-Based Affinity Chromatography -- Invariant Chain-Peptide Fusion Vaccine Using HER-2/neu -- TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens -- Production of Multiple CTL Epitopes from Multiple Tumor-Associated Antigens -- Preparation of Polypeptides Comprising Multiple TAA Peptides -- Idiotype Vaccine Production Using Hybridoma Technology -- Preparation of Cancer-Related Peptide Cocktails that Target Heterogeneously-Expressed Antigens -- Making an Avipoxvirus Encoding a Tumor-Associated Antigen and a Costimulatory Molecule -- Bacterial Vectors for the Delivery of Tumor Antigens -- Preparation of Peptide Microspheres Using Tumor Antigen-Derived Peptides -- Production of Antigen-Loaded Biodegradable Nanoparticles and Uptake by Dendritic Cells -- Development of Plasmid-Lipid Complexes for Direct Intratumoral Injection -- The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy -- Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines -- Challenges Facing the Development of Cancer Vaccines -- Future of Cancer Vaccines
    Abstract: Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells, as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells.℗ This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future
    Pages: XVIII, 569 p. 80 illus., 42 illus. in color. : online resource.
    ISBN: 9781493903450
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  • 7
    Keywords: Medicine ; Neurosciences ; Toxicology ; Cardiology ; Biochemistry ; Cell Membranes ; Biomedicine ; Pharmacology/Toxicology ; Neurosciences ; Cardiology ; Membrane Biology ; Protein Structure ; Springer eBooks
    Description / Table of Contents: Overview: biophysical properties and structure of sodium channels.-℗ The voltage sensor module in sodium channels -- Slow inactivation of Na+ channels -- Regulation/modulation of sensory neuron sodium channels -- Ubiquitylation of voltage-gated sodium channels -- Probing gating mechanisms of sodium channel using pore blockers -- Animal toxins influence voltage-gated sodium channel function -- Voltage-sensor trapping toxins: Iso form-specific ligands for sodium channels -- Pharmacological insights and quirks of bacterial sodium channels -- The role of non-pore-forming Îø subunits in physiology and pathophysiology of voltage-gated sodium channels -- The role of late INa in development of cardiac arrhythmias -- Proton modulation of cardiac INa: A potential arrhythmogenic trigger -- Altered sodium channel gating as molecular basis for pain: Contribution of activation, inactivation and resurgent currents.℗
    Abstract: A number of techniques to study ion channels have been developed since the electrical basis of excitability was first discovered. Ion channel biophysicists have at their disposal a rich and ever-growing array of instruments and reagents to explore the biophysical and structural basis of sodium channel behavior. Armed with these tools, researchers have made increasingly dramatic discoveries about sodium channels, culminating most recently in crystal structures of voltage-gated sodium channels from bacteria. These structures, along with those from other channels, give unprecedented insight into the structural basis of sodium channel function. This volume of the Handbook of Experimental Pharmacology will explore sodium channels from the perspectives of their biophysical behavior, their structure, the drugs and toxins with which they are known to interact, acquired and inherited diseases that affect sodium channels and the techniques with which their biophysical and structural properties are studied
    Pages: VIII, 295 p. 58 illus., 36 illus. in color. : online resource.
    ISBN: 9783642415883
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  • 8
    Keywords: Medicine ; Human Physiology ; Immunology ; Pneumology ; Biomedicine ; Human Physiology ; Immunology ; Pneumology/Respiratory System ; Springer eBooks
    Description / Table of Contents: Mitochondrial Genetic Abnormalities after Radiation Exposure -- Crediting Six Discoverers of Oxygen -- Hypoxia in Tumors: Pathogenesis-related Classification, Characterization of Hypoxia subtypes, and Associated Biological and Clinical Implications -- Heterogeneity in Tissue Oxygenation: From Physiological Variability in Normal Tissues to Pathophysiological Chaos in Malignant Tumours -- Oxygen Diffusion: An Enzyme-controlled Variable Parameter -- Role of Microvascular Shunts in the Loss of Cerebral Blood Flow Autoregulation -- Impact of Hypoxia-related Tumor Acidosis on Cytotoxicity of Different Chemotherapeutic Drugs in vitro and in vivo -- The Founding of ISOTT: The Shamattawa of Engineering Science and Medical Science -- A Tale of Two Methods: Combining Near-Infrared Spectroscopy with MRI for Studies of Brain Oxygenation and Metabolism -- Advances in Probes and Methods for Clinical EPR Oximetry -- Real-Time, In Vivo Determination of Dynamic Changes in Lung and Heart Tissue Oxygenation using EPR Oximetry -- Modulation of Hypoxia by Magnetic Nanoparticle Hyperthermia to Augment Therapeutic Index -- Skeletal Muscle and Glioma Oxygenation by Carbogen Inhalation in Rats: A Longitudinal Study by EPR Oximetry using Single-Probe Implantable Oxygen Sensors -- Recurrent Low-dose Chemotherapy to Inhibit and Oxygenate Head and Neck Tumors -- How in vivo EPR Measures and Images Oxygen -- What We Learn From In Vivo EPR Oxygen Images -- EPR Image Based Oxygen Movies for Transient Hypoxia -- Repetitive Measurements of Intrarenal Oxygenation in vivo using L band Electron Paramagnetic Resonance -- Quantitative Hypoxia Imaging for Treatment Planning of Radiotherapy -- A New Flavonoid Regulates Angiogenesis and Reactive Oxygen Species Production -- Angiotensin II Reduces Transport-Dependent Oxygen Consumption but Increases Transport-Independent Oxygen Consumption in Immortalized Mouse Proximal Tubular Cells -- Investigation of Cerebral Autoregulation in the Newborn Piglet during Anaesthesia and Surgery -- Influence of the Maternal use of Labetalol on the Neurogenic Mechanism for Cerebral Autoregulation Assessed by Means of NIRS -- Development of a Near Infrared Multi-Wavelength, Multi-channel, Time-Resolved Spectrometer for Measuring Brain Tissue Haemodynamics and Metabolism -- Simulating NIRS and MRS measurements during cerebral hypoxia-ischaemia in piglets using a computational model -- Analysis of Slow Wave Oscillations in Cerebral Haemodynamics and Metabolism Following Subarachnoid Haemorrhage -- Effects of Enriched Environment on Hippocampal Neuronal Cell Death and Neurogenesis in Rat Global Ischemia -- Automated Image Analysis for Diameters and Branching Points of Cerebral Penetrating Arteries and Veins Captured with Two-Photon Microscopy -- Cerebral Hemodynamic Changes and Metabolic Alteration in Severe Hemorrhagic Shock -- Physiological Mechanism of Increase in Deoxy-hemoglobin Concentration during Neuronal Activation in Patients with Cerebral Ischemia: A Simulation Study with the Balloon Model -- Effect of Blood in the Cerebrospinal Fluid on the Accuracy of Cerebral Oxygenation Measured by Near Infrared Spectroscopy -- Vessel Specific Imaging of Glucose Transfer with Fluorescent Glucose Analogue in Anesthetized Mouse Cortex -- Ischemic Pretreatment Delays Ischemic Brain Vasospasm Injury in Gerbils -- Changes in Cerebral Blood Oxygenation Induced by Active Standing Test in Children with POTS and NMS -- Optical Imaging of Brain Activation in Gambian Infants -- Asymmetrical Changes in Cerebral Blood Oxygenation Induced by an Active Standing Test in Children with Postural Tachycardia Syndrome -- Changes of Cerebral Tissue Oxygen Saturation at Sleep Transitions in Adolescents -- Influence of Subjective Happiness on the Prefrontal Brain Activity: An fNIRS Study -- Ginkobiloba Extract Improves Working Memory Performance in Middle-aged women; Role of Asymmetry of Prefrontal Cortex Activity during a Working Memory Task -- Bayesian Prediction of Anxiety Level in Aged People at Rest using 2-Channel NIRS Data from Prefrontal Cortex -- Short-term Hypoxic Preconditioning Improved Survival following Cardiac Arrest and Resuscitation in rats -- Venular Valves and Retrograde Perfusion -- Monitoring of Filter Patency during Carotid Artery Stenting using Near-Infrared Spectroscopy with High Time-Resolution -- Use of NIRS to Assess Effect of Training on Peripheral Muscle Oxygenation Changes in Elite Rugby Players Performing Repeated Supramaximal Cycling Tests -- Skeletal Muscle Deoxygenation Responses during Treadmill Exercise in Children -- Development of a Hybrid Microwave-Optical Thermoregulation Monitor for the Muscle -- Evaluation of a Textile-Based Near Infrared Spectroscopy System in Calf Muscle Oxygenation Measurements -- Skin Temperature in Lower Hind Limb Subjected to Distal Vein Arterialization in Rats -- Index
    Abstract: This book contains the refereed contributions from the 41st annual meeting of ISOTT. The annual meetings of ISOTT bring together scientists from various fields (medicine, physiology, mathematics, biology, chemistry, physics, engineering, etc.) in a unique international forum. Traditionally, ISOTT conferences are a place, where an atmosphere of interaction is created, where many questions are asked after each presentation and lively discussions occur at a high scientific level. This vivid interaction is the main motivation for members to participate and gain new ideas and knowledge in the broad field of oxygen transport to tissue. The papers in this volume summarize some of the outstanding contributions from the 41st annual meeting. Special features in this volume include invited presentations from senior members of ISOTT for the theme “the wisdom of ISOTT” in which founders, past presidents and prize winners from previous meetings provided both cutting edge new knowledge and integrated overviews of critical aspects of the field. The presentations and manuscripts also include those provided by the special opportunity provided by having part of the ISOTT meeting overlap with the EPR-2013 meeting where both focused on preclinical and clinical measurements of oxygen, with a particular emphasis on cancer
    Pages: XXX, 372 p. 152 illus., 60 illus. in color. : online resource.
    ISBN: 9781493906208
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  • 9
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    New York, NY : Springer
    Keywords: Life sciences ; Biotechnology ; Cytology ; Stem Cells ; Life sciences ; Stem Cells ; Biotechnology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Signaling Pathways Maintaining Stemness in Adult Hematopoietic Stem Cells -- The Adult Stem Cell Niche -- Adult Stem Cells: Adult Skeletal Muscle Stem Cells -- Adult Cardiac Stem Cells: Identity, Location and Potential -- Adult Pituitary Stem Cells -- Toward Translating Molecular Ear Development to Generate Hair Cells from Stem Cells -- Adult Human Corneal Epithelial Stem Cells -- Adult Stem Cells in Teeth -- Adult Mammary Stem Cells: Identity, Location and Functional Assays -- Adult Ovary Stem Cells -- Adult Prostate Stem Cells -- Adult Lung Stem Cells -- Adult Liver Stem Cells -- Lineage-committed Pancreatic Progenitors and Stem Cells -- Adult Stromal (Skeletal, Mesenchymal) Stem Cells -- Regeneration after Injury - Activation of Stem Cell Stress Response Pathways to Rapidly Repair Tissues -- Molecular and Endocrine Mechanisms Underlying the Stem Cell Theory of Aging
    Abstract: Adult Stem Cells, second edition, takes a critical look at issues concerning the℗ developmental or differentiation potential for a variety of tissue types and for specific adult stem cell types. Since the first edition appeared a decade ago, our understanding of adult stem cells, and more specifically tissue-specific adult stem cells, has advanced tremendously. And an increased interest in regenerative medicine and potential stem cell applications℗ has driven a quest for better understanding of stem cell biology. In turn, this has spawned much activity on generation and utilization of more and better reagents to identify and isolate stem cells and stem cell-like subpopulations, and on assays elucidating their developmental or differentiation potential and functional integration with host tissues and organs. In this fully updated new edition, chapters cover topics ranging from signaling pathways maintaining stemness in hematopoietic cells to regeneration after injury and endocrine mechanisms underlying the stem cell theory of aging. Other chapters cover℗ stem cells by organ or system including pituitary, cardiac, epithelial, teeth, lung, ovary, prostate, liver, and many more.℗ Importantly, the authors of the chapters have not only summarized their successes, but have also summarized some of the difficulties that each particular field is still facing with respect to maximizing the utility of stem cells in clinical settings. Collectively, they impart both the excitement and challenges facing stem cell utilization for repair and regeneration℗ making this book essential reading for those involved in stem cell research as well as those involved in clinical assays
    Pages: XIII, 429 p. 29 illus., 28 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781461495697
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  • 10
    Keywords: Medicine ; Orthopedics ; Rheumatology ; Biomedicine ; Molecular Medicine ; Orthopedics ; Rheumatology ; Springer eBooks
    Description / Table of Contents: Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction -- Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients -- Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors -- Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice -- Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice -- In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets -- Characterization of microRNAs and Their Targets -- Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell -- Basic Techniques for Studies of iNKT Cells and MAIT Cells -- Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen -- Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis -- Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach -- Genome-Wide Genetic Study in Autoimmune Disease Prone Mice -- Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis -- Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples -- Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy
    Abstract: Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field
    Pages: X, 204 p. 65 illus., 31 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493904044
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  • 11
    Keywords: Life sciences ; Agriculture ; Botany ; Life sciences ; Plant Sciences ; Agriculture ; Springer eBooks
    Description / Table of Contents: Measuring Freezing Tolerance: Survival and Regrowth Assays -- Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays -- Conducting Field Trials for Freezing Tolerance Breeding in Cereals -- A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance -- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation -- Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions -- Identification of Arabidopsis Mutants with Altered Freezing Tolerance -- Infrared Thermal Analysis of Plant Freezing Processes -- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues -- Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images -- Proteomic Approaches to Identify Cold Regulated Soluble Proteins -- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins -- Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry -- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition -- Quantification of Superoxide and Hydrogen Peroxide in Leaves -- Estimating Ice Encasement Tolerance of Herbage Plants -- Characterization of Ice Binding Proteins from Sea Ice Algae -- Isolation and Characterization of Ice Binding Proteins from Higher Plants.℗
    Abstract: Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties.℗ Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches
    Pages: XI, 282 p. 75 illus., 49 illus. in color. : online resource.
    ISBN: 9781493908448
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  • 12
    Keywords: Life sciences ; Food science ; Microbiology ; Life sciences ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Sampling the Production/Processing Environment for Listeria -- Traditional Methods for Isolation of Listeria monocytogenes -- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR -- Serotype Assignment by Sero-Agglutination, ELISA and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes -- Ribotyping and Automated Ribotyping of Listeria monocytogenes -- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- Analysis of Listeria monocytogenes Subproteomes -- The Listeria Cell Wall and Associated Carbohydrate Polymers -- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria -- Virulence Characterization of Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method -- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes -- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR -- Genome Sequencing of Listeria monocytogenes -- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels -- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers -- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments
    Abstract: Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and, finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research
    Pages: X, 286 p. 42 illus., 17 illus. in color. : online resource.
    ISBN: 9781493907038
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  • 13
    Keywords: Medicine ; Oncology ; Gene Expression ; Proteomics ; Biomedicine ; Cancer Research ; Molecular Medicine ; Proteomics ; Biomedicine general ; Gene Expression ; Springer eBooks
    Description / Table of Contents: Laser Capture Microdissection as a Tool for Compartmentalization Studies -- Histological Evaluation of the Normal Breast -- Methodology for Studying the Compartments of the Human Breast -- Methodological approach to Tissue Micro Array for Studying the Normal and Cancerous Human Breast -- In Vitro Techniques for Studying the Normal Breast and the use of Cell Lines in Breast Cancer Research -- In situ Methods for Indentifying the Stem Cell of the Normal and Cancerous Breast -- Imaging Techniques for Evaluation in vitro Behavior of Normal and Cancerous Breast Tissue -- In vivo Studies of Breast Cancer Cells -- RNA Sequencing in the Human Breast -- Methodological Approaches for the Understanding the Epigenetic Landscape of the of the Human Breast and its Implications in Cancer and Prevention
    Abstract: This book provides a detailed description of the most common techniques used in breast cancer research, with a special emphasis on the study of the normal breast as the basis for understanding the pathological processes in this organ. The authors have poured their experience in the field of breast cancer research into this book, and described the process of handling and interpreting the complex organ that is the human breast using original data and unique material collected over the years. ℗ They have established the concept of normality of the human breast and the morphological parameters to be used for distinguishing the normal from the diseased breast. The quality of the breast tissue obtained from autopsy material vs. core biopsies are carefully detailed and discussed, showing the morphological differences among the specimens according to their collection site. The use of laser capture microdissection and tissue microarray is described in detail and discussed with special references to the problems faced when the normal breast is under study. A chapter dedicated to the use of breast cancer cell lines is crafted around the importance of each cell line and its particularities for in vitro studies. In addition, the authors explore the new concept that cancer originates from two main cell types, basal and luminal cells . Particular attention is given to the study of the phenotypes commonly used for determining cell transformation, either spontaneously or by induced chemical agents. A companion chapter using in vivo models provides the adequate tools for those interested in studying the early events of cancer initiation, progression and metastasis. Special emphasis is given to the analysis of cells either by Flow cytometry and immuno-fluorescence techniques and the great potential of the use of in situ hybridization to study RNA transcripts. The book also features an original analysis of the putative markers of stem cells in breast cancer using immuno-cytochemical techniques, their interpretation and correlation with the prognosis of the disease. The last two chapters of the book are engaged in the utilization of RNA and DNA sequencing techniques and their values in understanding the physiological differences in the human breast, as well as the utilization of epigenetic methodologies in the understanding of the normal human breast. The authors have established the methodological basis for studying the human breast in its normal and pathological conditions providing a unique set of tools and valuable references for researchers pursuing a deeper understanding of the human breast
    Pages: XVI, 287 p. 192 illus., 167 illus. in color. : online resource.
    ISBN: 9781493907182
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  • 14
    Keywords: Medicine ; Neurosciences ; Psychiatry ; Neurobiology ; Biomedicine ; Neurosciences ; Psychiatry ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- Part 1. General Principles of Brain.-℗ Part 2.℗ Systems -- Part℗ 3.℗ Clinical Syndromes
    Abstract: Brain development, whether typical or atypical, provides the foundation for all behavior and possible psychopathology.℗ This volume takes a comprehensive and translational approach in describing how basic neuronal patterning occurs, organizes into systems, and forms functional and inter-connected networks.℗ The role that hormones and genes play in influencing brain development is also described.℗ The resultant emotional, cognitive, reward, and social systems show how typical development proceeds.℗ The second part of the book describes when the process of development goes awry.℗ Here, a number of childhood disorders are covered.℗ Autism, ADHD, emotional syndromes of bipolar disorder and depression, oppositional defiant disorder and conduct disorder, and sleep and schizophrenia are included in this volume. Written by leading experts in their respective fields, this volume will be a valuable resource to mental health professionals as well as preclinical investigators hoping to gain additional understanding about the neurobiology of the developing brain
    Pages: VIII, 370 p. 20 illus. in color. : online resource.
    ISBN: 9783642549137
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  • 15
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Structure and General Properties of Flavins -- Recent advances in Riboflavin Biosynthesis -- Natural Riboflavin Analogs -- A Roadmap to the Isotopolog Space of Flavocoenzymes -- Electron Transferases -- Aldonolactone Oxidoreductases -- Flavins and Flavoproteins: Applications in Medicine -- Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms -- On the in vivo Redox State of Flavin-Containing Photosensory Receptor Proteins -- Computational Spectroscopy, Dynamics and Photochemistry of Photosensory Flavoproteins -- NMR Spectroscopy on Flavins and Flavoproteins -- Solid-State NMR of Flavins and Flavoproteins.-℗ EPR on Flavoproteins -- FTIR Spectroscopy of Flavin-Binding Photoreceptors -- Resonance Raman Spectroscopy -- Photoactivation Mechanisms of Flavin-binding Photoreceptors Revealed through Ultrafast Spectroscopy and Global Analysis Methods -- Excited State Chemistry of Flavoproteins. ℗
    Abstract: In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins.℗ These include review style methods and protocols to exemplify the variety, the power, and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs, and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. ℗ Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes
    Pages: XII, 468 p. 136 illus., 70 illus. in color. : online resource.
    ISBN: 9781493904525
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  • 16
    Keywords: Medicine ; Oncology ; Angiography ; Cardiology ; Ophthalmology ; Biomedicine ; Molecular Medicine ; Oncology ; Cancer Research ; Angiology ; Cardiology ; Ophthalmology ; Springer eBooks
    Description / Table of Contents: Emergence of Endothelial Cells During Vascular Development -- Lymphatic Vascular Morphogenesis -- Finding New Partnerships; The Function of Individual Extracellular Receptor Domains in Angiogenic Signalling by VEGF Receptors -- Wnt/ Frizzled Signaling in the Vasculature -- BMP9, BMP10 And ALK1: An Emerging Vascular Signaling Pathway With Therapeutic Applications -- Apeling Signaling in Retinal Angiogenesis -- Emerging Role of the Two Related Basic Helix-Loop-Helix Proteins -- TAL1 and LYL1 In Angiogenesis -- Hypoxia and Extracellular Matrix Remodeling -- Sphingosine-1-Phosphate in Hypoxic Signaling -- Reciprocal Crosstalk Between Angiogenesis and Metabolism -- Endothelial Progenitor Cells and Cardiovascular Ischemic Diseases: Characterization, Functions and Potential Clinical Applications as Biomarker and Therapeutic Tools -- Endothelial Cell Reactions to Oxygen: Implications For Cancer -- Tumor Angiogenesis and Lymphangiogenesis: Microenvironmental Soil For Tumor Progression And Metastatic Dissemination -- Angiogenesis And Invasion in Malignant Glioma: Friends or Foes? -- Role of Endothelial Cells in Tumor Escape From Immunity -- Tumor High-Endothelial Venules and Lymphocyte Trafficking -- Post-Transcriptional Regulation of Angiogenesis Through AU-Rich Mrna Degradation: Potential Application in Cancer Therapy -- Antiangiogenic Drugs in Cancer Therapy, Effect on Advanced Renal Cell Carcinoma -- Anti-Angiogenic Therapies in Colorectal Cancer -- Resistance to Anti-Angiogenic Treatments via Up-Regulation of Substitution Pathways -- VEGF-A Splice Variants: Do They Play a Role in Tumor Responses to Anti-Angiogenic Therapies? -- Progenitor Endothelial Cells as Biomarkers of Anti-Vascular Agents -- Soluble Vascular Endothelial (VE)-Cadherin : Toward a Marker of Endotelial Dysfunction -- The Prognostic and Predictive Value of VEGF Across Various Tumor Types
    Abstract: Angiogenesis is a multi-stage process that drives the generation of new blood and lymphatic vessels from pre-existing ones. It is highly active during embryogenesis, largely inactive during adulthood but reactivated during wound healing and under a number of pathological conditions including cancer and ocular diseases. In addition to endothelial cells, which line the walls of the vessels, several other cell types (pericytes, macrophages, progenitor cells,…) also contribute to angiogenesis. A number of signaling pathways are activated and very finely tune the delicate morphogenetic events that ultimately lead to the formation of stable bloodproof neovessels. This book reviews recent advances in our understanding of the molecular and cellular mechanisms of angiogenesis, with a focus on how to integrate these observations into the context of developmental, post-natal and pathological neovascularization. The book was published under the auspices of the French Angiogenesis Society. Most contributors are prominent members of this Society or international researchers who have actively contributed to the Annual Meetings of the Society
    Pages: XI, 505 p. 64 illus., 58 illus. in color. : online resource.
    ISBN: 9782817804668
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  • 17
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    New York, NY : Springer
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Description / Table of Contents: Basic Investigations in Saccharomyces cerevisiae -- Isolation of Yeast Nucleic Acids -- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe -- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry -- Yeast Two Hybrid Liquid Screening -- Targeted Mutagenesis of a Specific Gene in Yeast -- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae -- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy -- Assays for Autophagy II: Mitochondrial Autophagy -- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction -- Yeast Survival and Growth Assays -- Spontaneous Mutagenesis Assay -- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene -- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe -- Replicative and Chronological Life Span Assays -- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae -- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae -- Ligand Engineering Using Yeast Surface Display -- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
    Abstract: Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism
    Pages: X, 313 p. 57 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493907991
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  • 18
    Keywords: Life sciences ; Botany ; Life sciences ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Measurement of Luciferase Rhythms -- Online Period Estimation and Determination of Rhythmicity in Circadian Data, using the BioDare Data Infrastructure -- Global℗ profiling of the circadian transcriptome using microarrays -- ChIP-Seq analysis of histone modifications at the core of the Arabidopsis circadian clock -- Quantitative transcriptome analysis using RNA-seq -- Rapid and parallel quantification of small and large RNA species -- The RIP per case ́€“ identification of RNA binding protein targets by RNA immunoprecipitation -- A protocol for visual analysis of alternative splicing in RNA-Seq data using Integrated Genome Browser -- AthaMap web tools for the analysis of transcriptional and post-transcriptional gene expression regulation in Arabidopsis thaliana -- Analysis of mRNA translation states in Arabidopsis over the diurnal cycle by polysome microarray -- Immunoprecipitation-based analysis of protein-protein interactions -- Comparative Phosphoproteomics to Identify Targets of the Clock-Relevant℗ Casein Kinase 1 in C. reinhardtii Flagella -- Pulsed induction of circadian clock genes in Arabidopsis seedlings -- The use of fluorescent proteins to analyze circadian rhythms -- Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana -- Circadian life without micronutrients: effects of altered micronutrient supply on clock function in Arabidopsis -- Assessing redox state and reactive oxygen species in circadian rhythmicity -- Circadian regulation of plant immunity to pathogens -- Determination of photoperiodic flowering time control in Arabidopsis and barley -- The perennial clock is an essential timer for seasonal growth events and cold hardiness -- Monitoring seasonal bud set, bud burst and cold hardiness in Populus -- Transformation and Measurement of Bioluminescence Rhythms in the Moss Physcomitrella patens -- Modeling and Simulating the Arabidopsis thaliana Circadian Clock Using XPP-AUTO
    Abstract: Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. ℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general
    Pages: XI, 361 p. 77 illus., 47 illus. in color. : online resource.
    ISBN: 9781493907007
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  • 19
    Keywords: Life sciences ; Microbiology ; Bacteriology ; Life sciences ; Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Gene Transfer: Transduction -- Gene Transfer: Transformation/Electroporation -- Gene transfer: Conjugation -- Pseudomonas Bacteriophage Isolation and Production -- Genotyping Methods -- Drug Susceptibility Testing by Dilution Methods -- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa -- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa -- Motility Assay: Twitching Motility -- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis -- Microscopic Analysis: Morphotypes and Cellular Appendages -- Determination of Lipolytic Activities -- Elastinolytic and proteolytic enzymes -- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins -- Cell Fractionation -- Characterization of Molecular Interactions using Isothermal Titration Calorimetry -- Proteomic Analysis -- Membrane Proteomics of Pseudomonas aeruginosa -- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays -- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules -- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules -- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP -- Metabolic Footprinting: Extracellular Metabolomic Analysis -- Pyoverdine and Pyochelin Measurements -- Measurement of Phenazines in Bacterial Cultures -- Extraction and Measurement of NAD(P)+ and NAD(P)H -- Cyanide Measurements in Bacterial Culture and Sputum -- Monitoring Iron Uptake by Siderophores -- Exopolysaccharide Quantification -- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids -- LPS quantitation Procedures -- Monitoring Lectin Interaction with Carbohydrates -- Mining the Pseudomonas Genome -- Identification of Bacterial Small RNAs by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis -- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics -- Signature Tagged Mutagenesis -- Construction of a Pseudomonas aeruginosa Genomic Bank -- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes -- Promoter Fusions with Optical Outputs in Individual Cells and in Populations -- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq -- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans -- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop -- Biofilm Formation in the 96-well Microtiter Plate -- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa -- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans -- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum -- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella -- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana -- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells -- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish -- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster -- Assessing Pseudomonas Virulence using Host Cells -- Assessing Pseudomonas aeruginosa℗ Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection -- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model -- Burn Mouse Models
    Abstract: In℗ Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings
    Pages: XVIII, 816 p. 157 illus., 89 illus. in color. : online resource.
    ISBN: 9781493904730
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  • 20
    Keywords: Medicine ; Gene Expression ; Human Physiology ; Pneumology ; Biochemistry ; Biomedicine ; Human Physiology ; Medical Biochemistry ; Molecular Medicine ; Protein Science ; Gene Expression ; Pneumology/Respiratory System ; Springer eBooks
    Description / Table of Contents: Epigenetics in Childhood Health and Disease -- Role of Epigenetics in Neural Differentiation: Implications for Health and Disease -- An Overview of Epigenetic Mechanisms in Health and Disease -- Epigenetics: Role of Histone Proteases in Cellular Functions and Diseases -- Anti-inflammatory Effects of Probiotics and their Metabolites – Possible Role for Epigenetic Effects -- Epigenetics of Autoimmune Diseases -- The Effect of Nutrition and Exercise on Epigenetics and the Development of Cardiovascular Disease -- Epigenetic Events Associated with Obesity and Diabetes -- Molecular Mechanisms in the Development and Progression of Asthma: The Role of Epigenetic Regulation and the Airway Epithelium -- The Significance of Nanoparticles in Medicine and their Potential in Asthma -- One-carbon Metabolism Nutrients and Epigenetics: A Mechanistic Link Between Aberrant One-carbon Metabolism and Cancer Risk? -- Principles of the Warburg Effect and Cancer Metabolism -- Molecular Aspects of the Warburg Effect -- Epigenetic Perturbations in the Context of the Multi-hit Hypothesis of Carcinogenesis -- Epigenetic Mechanisms of Colon Cancer Prevention -- Dietary Antioxidants and Chromatin Modifying Compounds as Potential Anticancer Therapies -- Combination Therapy for Cancer – Phototherapy and HDAC Inhibition -- Nano-base Drug Delivery Modalities for the Treatment of Cancer: The Formulation of Tumour-specific and –targeted Nanoparticles
    Abstract: In a simplified form, epigenetics refers to heritable changes in phenotype that are not due to changes in the underlying DNA sequence. In this book, epigenetic mechanisms of regulation and dysregulation in health and disease are explored in great depth. Detailed chapters on epigenetic processes including DNA methylation and chromatin post-translational modifications including potential interventions with DNA methyltransferase inhibitors and histone deacetylase inhibitors are explored in initial chapters. These provide a detailed overview and important background to the entire field. The book is then focussed on epigenetic mechanisms involved in various diseases including anti-inflammatory and autoimmune conditions. Important accounts relating to the effects of epigenetics in metabolic syndrome, cardiovascular disease and asthma are the focus of subsequent chapters. The role of epigenetic dysregulation in malignancy is a current topic of interest and represents an intense field of research. A large component of this book is dedicated to the analysis of aberrant epigenetic processes in carcinogenesis and cancer progression. Further, chapters are focused on emerging cancer prevention using nutritional components and anti-cancer therapies particularly with histone deacetylase inhibitors, which have already been approved for the treatment of cutaneous T-cell lymphoma. The emerging role of nanoparticle preparations, especially in the context of delivering potential epigenetic therapies to target cells in various diseases, is also explored in this book. Overall, this book encompasses a wide range of topics related to epigenetic mechanisms in health and disease and would appeal to anyone with an interest in epigenetics, chromatin biology and emerging epigenetic interventions and therapies
    Pages: XIV, 505 p. 43 illus., 33 illus. in color. : online resource.
    ISBN: 9781493907069
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  • 21
    Keywords: Medicine ; Neurosciences ; Medical virology ; Public Health ; Neurology ; Biomedicine ; Neurosciences ; Virology ; Public Health ; Neurology ; Springer eBooks
    Description / Table of Contents: Overview on neglected and tropical diseases -- Travel, migration and neglected tropical diseases -- Stigma in neurological diseases at the Tropics -- Nematode infections: Neurological involvement and neurobiology -- Amoebiasis: Neurological involvement and neurobiology -- Neuroschistosomiasis -- Neurocysticercosis -- Neurocysticercosis: Neurology and neurobiology -- Onchocerciasis: Neurological involvement -- Human African trypanosomiasis: Neurobiology -- Chagas' disease: Neurology and neurobiology -- Neurodegeneration in leprosy: Insights from model systems and patients -- Rabies: Neurology -- Rabies: Neurobiology -- Japanese encephalitis and other arbovirus infections: Neurology and neurobiology -- Khat addiction: Neurology -- Khat addiction: Neurobiology -- Konzo: neurology of a permanent and non-progressive motor neuron -- Food preservation, snake venoms and stroke in the Tropics -- Infectious causes of epilepsy? -- Cognitive impairment and behavioural disturbances following malaria or HIV infection in childhood
    Abstract: A number of diseases and conditions that occur primarily in remote rural or poor urban areas of low-income countries have traditionally been neglected by the neuroscience research community. These diseases and conditions affect the nervous system directly (sometimes with lethal consequences) and/or are associated with severe neurological sequels such as epilepsy, cognitive deficits, and sleep disruption. Several diseases also have the effect of promoting poverty by leaving sufferers unable to lead economically productive lives due to cognitive and behavioral disturbances or severe stigmatization. The pathogenesis of neural dysfunction in the diseases addressed in this book and their sequels remains unclear. Neuroscience of Neglected Diseases and Conditions makes available much needed information about how these diseases affect the human nervous system as well as to promote interest in further research. Further research into neglected diseases and conditions will uncover information that sheds light on more general topics of interest to the neuroscience research community
    Pages: VIII, 401 p. 122 illus., 51 illus. in color. : online resource.
    ISBN: 9781461481003
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  • 22
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    Heidelberg : Springer
    Call number: QZ365WJ358:137(6) ; M100:542
    Keywords: Urogenital Neoplasms
    Notes: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Pages: xxii, 883 p. : ill.
    Edition: 6., vollständig überarbeitete Aufl.
    ISBN: 978-3-642-35031-3
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  • 23
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Protein Science ; Protein Structure ; Springer eBooks
    Description / Table of Contents: 1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins -- Affinity Purification of Heme-Tagged Proteins -- Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag -- Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag -- Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag -- SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1 -- Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag -- Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins -- Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2 -- Detection of Protein-Protein Interactions Using Tandem Affinity Purification -- An Improved in vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells -- Purification of Recombinant Proteins with a Multifunctional GFP Tag -- Targeted Purification of SnAvi-Tagged Proteins -- An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells -- Bimolecular Affinity Purification - A Variation of TAP with Multiple Applications
    Abstract: Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given proteiń€™s function and structure. Todaý€™s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to ́€œfish out́€ a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information ́€“ information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of todaý€™s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest
    Pages: X, 213 p. 46 illus., 31 illus. in color. : online resource.
    ISBN: 9781493910342
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  • 24
    Keywords: Life sciences ; Botany ; Life sciences ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling -- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR -- Analysis of MAPK Activities using MAPK-specific Antibodies -- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels -- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™ -- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae -- Virus-induced Gene Silencing in Plant MAPK Research -- RNA Interference of Plant MAPK Cascades for Functional Studies -- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes -- Immunofluorescent Localization of MAPKs in Steedman’s Wax Sections -- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies -- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases -- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1 -- In vivo Phosphorylation of WRKY Transcription Factor by MAPK -- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates -- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome -- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays
    Abstract: Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students
    Pages: XI, 266 p. 33 illus., 17 illus. in color. : online resource.
    ISBN: 9781493909223
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  • 25
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    New York, NY : Springer
    Keywords: Medicine ; Medical laboratories ; Emerging infectious diseases ; Medicine & Public Health ; Infectious Diseases ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Hemagglutination Assay for Influenza Virus -- Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus -- Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity -- Reverse Genetics of℗ Influenza Virus -- Immunohistochemical Staining of Influenza Virus in Tissues -- A Brief Introduction to Avian Influenza Virus -- Wild Bird Surveillance for Avian Influenza Virus -- Avian Influenza Virus Sample Types, Collection and Handling -- Avian Influenza Virus RNA Extraction -- Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR -- Influenza Subtype Identification with Molecular Methods -- Avian Influenza Virus Isolation, Propagation and Titration in Embryonated Chicken Eggs -- Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus -- Detection of Influenza A Antibodies in Avian Serum Samples by ELISA -- Conducting Influenza Virus Pathogenesis Studies in Avian Species -- Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry -- Detection of Cell Mediated Immune Response to Avian Influenza Viruses -- Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR -- Newcastle Disease Virus Detection and Differentiation from Avian Influenza -- A Brief Introduction to Influenza A Virus in Swine -- Sample Types, Collection, and Transport for Influenza A Viruses of Swine -- Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs -- RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR -- Hemagglutinin Inhibition Assay with Swine Sera -- Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype Specific IgG and IgA Whole Virus Antibody to Influenza A Virus in Swine -- Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine -- Microneutralization Assay for Swine Influenza Virus in Swine Serum -- Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA) -- Antibody Secreting Cell Assay for Influenza A Virus in Swine -- In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza -- A Brief Introduction to Equine Influenza and Equine Influenza Viruses -- Equine Influenza Diagnosis: Sample Collection and Transport -- RNA Extraction from Equine Samples for Equine Influenza Virus -- Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR -- Equine Influenza Culture Methods -- Equine Influenza Serological Methods
    Abstract: Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies. Influenza A viruses are among the most important pathogens for humans, food animals and companion animals. Research and diagnostics with animal influenza viruses are critical to animal health and it should be recognized that the needs and goals of animal agriculture and veterinary medicine are not always the same as those of public health.℗ This volume sorts out the differences in the structure of the poultry, swine and equine industries, the biological differences of influenza virus from each animal group, and provides host, strain and lineage specific guidance and procedures. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and accessible, Avian Influenza Virus, Second Edition seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of these important pathogens
    Pages: XII, 425 p. 58 illus., 48 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493907588
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  • 26
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: Derivation and Network Formation of Vascular Cells from Human Pluripotent Stem Cells -- High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis -- Microfluidic Device to Culture 3D In Vitro Human Capillary Networks -- Multi-Function Co-Culture Model for Evaluating Cell-Cell Interactions -- Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography -- Bioreactor Cultivation of Anatomically-Shaped Human Bone Grafts -- Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells -- Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules -- An Assay to Quantify Chemotactic Properties of Degradation Products from Extracellular Matrix -- Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment -- Fabrication of Biofunctionalized, Cell-Laden Macroporous 3D PEG Hydrogels as Bone Marrow Analogs for the Cultivation of Human Hematopoietic Stem and Progenitor Cells -- Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation -- The Delivery and Evaluation of RNAi Therapeutics for Heterotopic Ossification Pathologies -- Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation -- Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in Perfusion Bioreactors
    Abstract: Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to ́€˜́€˜instruct́€™́€™ stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and essential, Biomimetics and Stem Cells: Methods and Protocols focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward
    Pages: XI, 187 p. 54 illus., 31 illus. in color. : online resource.
    ISBN: 9781493913329
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  • 27
    Keywords: Life sciences ; Veterinary Medicine ; Entomology ; Life sciences ; Entomology ; Veterinary Medicine ; Springer eBooks
    Description / Table of Contents: 1 Introduzione: cenni storici sulle patologie delle api, diffusione geografica e impatto economico delle principali patologie delle api, nozioni di epidemiologia, profilassi e terapia -- 2 Difese naturali della colonia d€™ápi contro le patologie: difese individuali e immunit© sociale, recenti acquisizioni sui meccanismi molecolari di difesa contro le infezioni batteriche -- 3 Malattie batteriche: peste americana, peste europea, setticemie e altre infezioni batteriche -- 4 Virosi: le infezioni virali delle api, tecniche diagnostiche per la ricerca dei virus delle api -- 5 Micosi: ascosferosi, aspergillosi, bettsia alvei -- 6 Nosemiasi -- 7 Malattie da protozoi: amebiasi, altri protozoi associati alle api -- 8 Acari parassiti: varroasi, acariasi respiratoria, altri acari associati alle api -- 9 Insetti parassiti e predatori (lepidotteri, ditteri, coleotteri, imenotteri) -- 10 Predatori dell€™álveare (anfibi e rettili, uccelli, mammiferi) -- 11 Anomalie, disordini di origine incerta, malattie non parassitarie, patologia dell€™ápe regina -- 11 Avvelenamenti da agrofarmaci -- 12 Problemi legati ai trattamenti farmacologici -- 13 Approccio integrato alla gestione sanitaria degli apiari -- 14 Legislazione sanitaria in apicoltura
    Abstract: In questo volume vengono descritti i numerosi aspetti della patologia delle api con prospettive diverse e molteplici, tali da raggiungere destinatari diversi: l€™ápicoltore, il tecnico addetto all€™ássistenza sanitaria, il veterinario, il naturalista, il ricercatore, lo studente. Accanto alle nozioni pi©£ aggiornate, infatti, sono sempre presenti un gran numero di indicazioni operative. A un livello tecnico-scientifico vengono affrontate tutte le patologie conosciute delle api e, pi©£ in generale, le problematiche sanitarie dell€™ápicoltura: dall€™épidemiologia alla normativa, dalle avversit© ambientali ai residui nei prodotti dell€™álveare. Le indicazioni tecniche, soprattutto nel campo della profilassi, rendono questo volume particolarmente interessante anche dal punto di vista pratico, anche grazie a un apparato iconografico particolarmente ricco
    Pages: XX, 410 pagg. : online resource.
    ISBN: 9788847056503
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  • 28
    Keywords: Life sciences ; Agriculture ; Microbial genetics ; Plant Diseases ; Plant breeding ; Life sciences ; Microbial Genetics and Genomics ; Agriculture ; Plant Pathology ; Plant Breeding/Biotechnology ; Springer eBooks
    Description / Table of Contents: ́€œGenomics of Erwinia amylovora and Related Erwinia Species Associated with Pome Fruit Treeś€ by Youfu Zhao -- ́€œGenomics of Plant-Associated Bacteria: The Soft Rot Enterobacteriacea逝 by Amy O. Charkowski, Jenna Lind and Isael Rubio-Salazar -- €œÉcological Genomics of Pseudomonas syringa逝 by David A. Baltrus, Tory A. Hendry and Kevin L. Hockett -- ́€œPseudomonas syringae Genomics:℗ From Comparing Individual Strains to Analyzing Entire Populationś€ by Boris A. Vinatzer, Caroline L. Monteil and David J. Studholme -- ́€œGenetics and Functional Genomics of the Pseudomonas fluorescens Grouṕ€ by Sarah Craven Seaton and Mark W. Silby -- ́€œThe Genomics of Xanthomonas oryza逝 by Lindsay Triplett, Ralf Koebnik, Valerie Verdier and Jan E. Leach -- ́€œGenomics of Xanthomonas citri and Related Specieś€ by Neha Jalan, Qing Yan, Sunitha Kogenaru, Yinping Guo, Jeffrey B. Jones, James H. Graham and Nian Wang -- ́€œGenomic Insights into Xylella fastidiosa Interactions with Plant and Insect Hostś€ by Adam C. Retchless, Fabien Labroussaa, Lori Shapiro, Drake C. Stenger, Steven E. Lindow and Rodrigo P. P. Almeida -- ́€œComparative Genomics of the Liberibacteral Plant Pathogenś€ by Hong Lin and Edwin L. Civerolo -- ́€œPhytoplasma Genomes: Evolution through Mutually Complementary Mechanisms, Gene Loss and Horizontal Acquisitioń€ by Yan Zhao, Robert E. Davis, Wei Wei, Jonathan Shao and Rasa Jomantiene
    Abstract: This book describes how genomics has revolutionized our scientific understanding of agriculturally important plant-associated bacteria. Each chapter focuses on the genomics of particular bacteria: the first described plant pathogen, Erwinia amylovora; phytoplasmas lacking cell walls; fastidious, phloem-restricted liberibacters; Pseudomonas syringae, which is a genetically tractable model system; Xanthomonas citri, which causes a disease that can devastate citrus crops; and Pseudomonas fluorescens, which can protect plants from diseases. Topics considered in this volume include the importance of horizontal gene transfer in originating new bacterial strains and species, and advances in transcriptomics that allow us to describe the complex regulatory networks critical to plant-microbe interactions. The availability of the Xanthomonas oryzae genome has led to new technologies in genome editing, which will revolutionize approaches to genetic engineering, even in eukaryotes. The contributions show how genomics has greatly accelerated progress toward understanding the biology of these bacteria and how that understanding can be translated into novel crop protection methods
    Pages: X, 278 p. 36 illus., 13 illus. in color. : online resource.
    ISBN: 9783642553783
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  • 29
    Keywords: Medicine ; Immunology ; Microbiology ; Vaccines ; Medical parasitology ; Biomedicine ; Parasitology ; Immunology ; Medical Microbiology ; Vaccine ; Springer eBooks
    Description / Table of Contents: Form and Function in the Digenea -- The Systematics of the Trematoda -- Schistosomiasis -- Fascioliasis -- Paragonimiasis -- Liver Flukes -- Intestinal Trematode Infections -- Epidemiology of Trematode Infections -- Diagnosis of Human Trematode Infections -- Schistosomatidae and Diplostomidea -- Amphistomes -- Dicrocoeliidae Family -- Selected Wildlife Trematodiasis
    Abstract: Digenetic trematodes constitute a major helminth group that parasitize humans and animals, and are a major cause of morbidity and mortality. The diseases caused by trematodes have been neglected for years, especially as compared with other parasitic diseases. However, the geographical limits and the populations at risk are currently expanding and changing in relation to factors such as growing international markets, improved transportation systems, and demographic changes. This has led to a growing international interest in trematode infections, although factors such as the difficulties entailed in the diagnosis, the complexity of human and agricultural practices, the lack of assessments of the economic costs, or the limited number of effective drugs are preventing the development of control measures of these diseases in humans and livestock. In-depth studies are needed to clarify the current epidemiology of these helminth infections and to identify new and specific targets for both effective diagnosis and treatments. The main goal of this book is to present the major trematodes and their corresponding diseases in the framework of modern parasitology, considering matters such as the application of novel techniques and analysis of data in the context of host-parasite interactions and to show applications of new techniques and concepts for the studies on digenetic trematodes. This is an ideal book for parasitologists, microbiologists, zoologists, immunologists, professional of public health workers, clinicians, and graduate and post-graduate students
    Pages: XI, 474 p. 45 illus., 41 illus. in color. : online resource.
    ISBN: 9781493909155
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  • 30
    Keywords: Life sciences ; Cytology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Notch Signaling -- Genetic Screens to Identify New Notch Pathway Mutants in Drosophila -- Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes -- Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice -- Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster -- Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila -- Tracking Trafficking of Notch and its Ligands in Mammalian Cells -- Visualizing Notch Signaling in vivo in Drosophila Tissues -- Monitoring Notch Activity in the Mouse -- Notch Signaling Assays in Drosophila Cultured Cell Lines -- Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays -- Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays -- Visualization of Notch Signaling Oscillation in Cells and Tissues -- Proteomic Analysis of the Notch Interactome -- Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands -- Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry -- Assay to Probe Proteolytic Processing of Notch by γ-secretase -- Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay -- Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay -- Probing the Epigenetic Status at Notch Target Genes -- Notch-ligand Binding Assays in Drosophila Cells -- Modeling Notch Signaling: A Practical Tutorial -- Small Molecules that Inhibit Notch Signaling -- Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling -- Application of Anti-ligand Antibodies to Inhibit Notch Signaling
    Abstract: Notch Signaling: Methods and Protocols focuses on molecular, biochemical, and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage, and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor
    Pages: XI, 351 p. 59 illus., 46 illus. in color. : online resource.
    ISBN: 9781493911394
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  • 31
    Keywords: Medicine ; Human Physiology ; Neurosciences ; Biomedicine ; Neurosciences ; Human Physiology ; Springer eBooks
    Description / Table of Contents: History of Electrophysiology and the Patch-Clamp.-℗ Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications -- Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers -- State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening -- Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch℗ʼ 96 Using Instant Frozen Cells -- Planar Patch Clamp for Neuronal Networkś€“Considerations and Future Perspectives -- Methods for the Study of Synaptic Receptor Functional Properties -- Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity -- Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique -- Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability -- Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices -- Outside-Out ́€˜Sniffer-patch́€™ Clamp Technique for in situ Measures of Neurotransmitter Release -- A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices -- Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics -- Synaptic Connectivity in Engineered Neuronal Networks -- Modeling of Action Potential Generation in NG108-15 Cells -- Whole-Cell Patch-Clamp Recordings in Freely Moving Animals -- Pressure-Polished Borosilicate Pipettes are €œÚniversal Sealeŕ€ Yielding Low Access Resistance and Efficient Intracellular Perfusion -- Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes -- Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents -- Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI -- Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes -- Principles of Single-Channel Kinetic Analysis
    Abstract: Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies, and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology, and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals, and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Patch-Clamp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators
    Pages: XI, 403 p. 105 illus., 41 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493910960
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  • 32
    Keywords: Life sciences ; Biochemistry ; Cytology ; Botany ; Plant physiology ; Plant breeding ; Life sciences ; Plant Sciences ; Plant physiology ; Plant Breeding/Biotechnology ; Plant Biochemistry ; Cell Biology ; Earth System Sciences ; Springer eBooks
    Description / Table of Contents: Brassicas: Responses and Tolerance To Heavy Metal Stress -- Recent Advances in Rapid and Sensitive Screening For Abiotic Stress Tolerance -- Transcriptomics of Heat Stress in Plants -- Biotic Stress and Crop Improvement -- Salt Stress and Sugar Beet Improvement: Challenges and Opportunities -- Genotypic Variation for Drought Tolerance in Wheat Plants -- Soil Contaminants: Sources, Effects and Approaches for Remediation -- Role of Macronutrients in Plant Growth and Acclimation: Recent Advances and Future Prospective -- Mutation Breeding: A Novel Technique for Genetic Improvement of Pulse Crops Particularly Chickpea (Cicer arietinum L.) -- Organic Farming: The Return To Nature -- The Role of Cytological Aberrations in Crop Improvement Through Induced Mutagenesis -- Wheat Improvement: Historical Perspective and Mutational Approach-A Review -- Cotton Leaf Curl Virus Disease Predictive Model Based on Environmental Variables -- Transcription Factors in Abiotic Stress Responses -Their Potentials in Crop Improvement
    Abstract: Abiotic stress drastically limits agricultural crop productivity worldwide. Climate change threatens the sustainable agriculture with its rapid and unpredictable effects, making it difficult for agriculturists and farmers to respond to the challenges cropping up from environmental stresses.℗ In light of population growth and climate changes, investment in agriculture is the only way to avert wide scale food shortages. This challenge comes at a time when plant sciences are witnessing remarkable progress in understanding the fundamental processes of plant growth and development. Plant researchers℗ have identified℗ genes controlling different aspects of plant growth and development, but many challenges still exist in creating an apt infrastructure,℗ access to bioinformatics, and good crop results. Improvement of Crops in the Era of Climatic Changes, Volume℗ 2℗ focuses on many existing opportunities that can be applied methodically through conventional breeding, without touching upon the latest discoveries such as the power of genomics to applied breeding in plant biology.Written by a diverse℗ faction of internationally famed℗ scholars, this volume adds new horizons in the field of crop improvement, genetic engineering and abiotic stress tolerance. Comprehensive and lavishly illustrated, Improvement of Crops in the Era of Climatic Changes, Volume℗ 2℗ is a state-of-the-art guide to recent developments℗ vis-© -vis℗ various aspects of plant responses in molecular and biochemical ways to create strong yields and overall crop improvement
    Pages: XVII, 368 p. 34 illus., 12 illus. in color. : online resource.
    ISBN: 9781461488248
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  • 33
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    New York, NY : Springer
    Keywords: Life sciences ; Biological models ; Developmental Biology ; Plant Ecology ; Botany ; Plant anatomy ; Plant physiology ; Life sciences ; Plant Sciences ; Plant Ecology ; Systems Biology ; Developmental Biology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Introduction -- Definition of Celiac Disease and Gluten Sensitivity -- Epidemiology of Celiac Disease -- The Pathophysiology of Celiac Disease -- Immunogenetics of Celiac Disease -- Using Animal Models of Celiac Disease to℗ Understand the Role of MHC II -- Role of Gut Microbes in Celiac Disease Risk and Pathogenesis -- Presentation of Celiac Disease in Children and Adults -- Diagnosis of Celiac Disease -- Nutrition in Celiac Disease -- Dietary Supplements in Celiac Disease -- Monitoring and Follow-up of Patients with Celiac Disease -- Special Considerations in Children and Young Adults with Celiac Disease -- Novel and Experimental Therapies on the Horizon -- Morbidity and Mortality Associated with Celiac Disease -- Refractory Celiac Disease -- Celiac Disease: Dispelling Misconceptions and Myths -- Summary of Recommendations from the American College of Gastroenterology Celiac Disease Guidelines
    Abstract: This volume addresses recent developments in weed science. These developments include conservation agriculture and conservation tillage, climate change, environmental concerns about the runoff of agrochemicals, resistance of weeds and crops to herbicides, and the need for a vastly improved understanding of weed ecology and herbicide use. The book provides details on harnessing knowledge of weed ecology to improve weed management in different crops and presents information on opportunities in weed management in different crops. Current management practices are also covered, along with guidance for selecting herbicides and using them effectively. Written by experts in the field and supplemented with instructive illustrations and tables, Recent Advances in Weed Management is an essential reference for agricultural specialists and researchers, government agents, extension specialists, and professionals throughout the agrochemical industry, as well as a foundation for advanced students taking courses in weed science
    Pages: XI, 411 p. 36 illus., 23 illus. in color. : online resource.
    ISBN: 9781493910199
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  • 34
    Keywords: Medicine ; Neurosciences ; Neurochemistry ; Neurobiology ; Biomedicine ; Neurosciences ; Neurochemistry ; Neurobiology ; Springer eBooks
    Description / Table of Contents: The Blood Brain Barrier -- Immune Cell Trafficking in the Central Nervous System -- General Physiology and Pathophysiology of Microglia Inflammation and the Pathophysiology of Astrocytes in Neurodegenerative Diseases -- Oxidative stress, neuroinflammation, and neurodegeneration -- Peripheral Neuroimmune Interactions and Neuropathic Pain -- Innate and Adaptive Immune-Mediated Neuroinflammation and Neurodegeneration in Parkinson’s Disease -- Multiple sclerosis: impact on functioning of the blood-brain barrier -- Neuroinflammation in Alzheimer’s disease -- Neuroinflammation in Huntington’s Disease -- Neuroinflammation and immune regulation in ischemic stroke: identification of new pharmacological targets -- HIV/NeuroAIDS -- Herpes Simplex Virus Infections Of The Central Nervous System -- Congenital Cytomegalovirus Disease -- Japanese Encephalitis: A Tale of Inflammation and Degeneration in the Central Nervous System -- The biology and clinical consequence of infection with the human polyomavirus JCV -- Role of Retrovirus-Induced Transactivator Proteins in Neuroinflammatory Disease -- Immunopathogenesis of bacterial meningitis -- Cerebral Malaria -- HIV and Cocaine Interplay in HIV-associated Neurocognitive Disorders -- Methamphetamine Neurotoxicity and Neuroinflammatory Processes -- Drugs of Abuse and NeuroAIDS: Opiates -- Cannabinoids -- Alcohol and Neurodegeneration -- Cell-Based Drug Delivery for Improving Antiretroviral Therapeutic Outcomes -- Immunomodulatory Therapeutics for Neurodegenerative Diseases -- Therapeutic Potential Of Cannabinoids
    Abstract: State of the art reviews by experts in the fields of neuroscience, immunology, microbiology/infectious diseases, and pharmacology addressing the convergence of the immune system (neuroinflammation) and the loss of neurons (neurodegeneration).℗ Many of the diseases that are discussed in the book are of epidemic proportion, e.g., Alzheimeŕ€™s disease, Parkinsoń€™s disease, stroke, viral encephalitis, and substance abuse.℗ In addition to discussions of the involvement of neuroinflammation and neurodegeneration in these disorders, scientific reviews are presented on the cells and mediators that participate in defense of and damage to the nervous system.℗ ℗ With rare exception, no or inadequate treatment exists for the diseases discussed in this book.℗ An underlying premise of the book is that understanding of their shared pathogenic mechanisms will lead to improved therapies. Given the rapid evolution of the field of Neuroimmune Pharmacology, readers will find this book to be the most timely and authoritative reference on the subject of each of its chapters
    Pages: XVII, 600 p. 48 illus., 35 illus. in color. : online resource.
    ISBN: 9781493910717
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  • 35
    Keywords: Life sciences ; Geography ; Agriculture ; Sustainable development ; Life sciences ; Agriculture ; Climate Change/Climate Change Impacts ; Sustainable development ; Economic Geography ; Trade ; Springer eBooks
    Description / Table of Contents: 1. Population, agriculture, poverty and food security: an overview -- 2. Policies and legislations governing marketing and food trade: Southern Africa -- 3. Urban, peri-urban agriculture and food security among small-scale agricultural producers: Southern Africa -- 4. Indigenous plant resources and food security among small-scale agricultural producers: Southern Africa -- 5. Role of small stock in safeguarding food security in dry lands: case in Namibia -- 6. Improving productivity, food safety and nutritional levels of dependable food crops: Southern Africa -- 7. Climate-smart agriculture: incorporating weather information into small-scale agricultural producers' system: South Africa
    Abstract: The book focuses on food security highlighting the role of indigenous knowledge and scientific research in addressing the plight of poor small-scale agricultural producers. Rapidly growing global population and global policies and management governing sustainability, hunger, food security and poverty alleviation are discussed. Additionally, impacts of probable climate change, research on land productivity and performance of dependable food crops i.e. cassava and pearl millet are discussed. Analyzed in great detail are roles of small stock, urban/peri-urban agriculture and advantages of climate-smart agriculture and participatory research in enhancing food security of the small-scale agricultural producers in Southern Africa
    Pages: XVI, 173 p. 35 illus., 26 illus. in color. : online resource.
    ISBN: 9783319094953
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  • 36
    Keywords: Chemistry ; Biotechnology ; Microscopy ; Nanotechnology ; Chemistry ; Biotechnology ; Nanotechnology ; Biological Microscopy ; Springer eBooks
    Description / Table of Contents: Quantum Dots as Biophotonics Tools -- Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum Dots -- Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence -- Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots -- Quantum Dots-Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging -- Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation Spectroscopy -- Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens Spectroscopy -- Semiquantitative Fluorescence Method for Bioconjugation Analysis -- Assembly, Characterization and Delivery of Quantum Dot Labeled Biotinylated Lipid Particles -- Oriented Conjugation of Single-Domain Antibodies and Quantum Dots -- Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac Microenvironment -- In Vitro Approaches to Assessing the Toxicity of Quantum Dots -- Basics for the Preparation of Quantum Dots and their Interactions with Living Cells -- In Vivo Approaches to Assessing the Toxicity of Quantum Dots -- Quantum Dots for Imaging Neural Cells In Vitro and In VivoA Short-Term Inhalation Study Protocol – Designed for Testing of Toxicity and Fate of Nanomaterials -- Quantitative Measurement of Proteolytic Rates with Quantum Dot-Peptide Substrate Conjugates and Förster Resonance Energy Transfer -- Solid-Phase Supports for the in situ Assembly of Quantum Dot-FRET Hybridization Assays in Microfluidics
    Abstract: In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots(QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs℗þ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals, and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ ℗ Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research
    Pages: XI, 258 p. 76 illus., 65 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493912803
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  • 37
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Negative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8+ T Cells From Blood And Tumor Tissue -- In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes -- Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry -- ℗ Intracellular Signalling Of Ctls -- Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr -- Ctl Elispot Assay -- Methods Of Purification Of Ctl-Derived Exosomes -- Characterization Of Ctl By Microscopy -- Intravital Imaging Of Cytotoxic T Lymphocytes -- The Microarray-Based Approach For The Analysis Of The Transcriptome -- Mirnome Analysis Using Real Time Pcr -- Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis -- Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis -- Targeting ℗ The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs) -- Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption -- Ctl And Transplantation: Tissue In Vivo Characterization
    Abstract: ℗ In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. ℗ The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. ℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology
    Pages: XII, 301 p. 32 illus., 26 illus. in color. : online resource.
    ISBN: 9781493911585
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  • 38
    Keywords: Medicine ; Human Genetics ; Toxicology ; Biomedicine ; Pharmacology/Toxicology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use -- The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test -- Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles -- The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity -- The In Vitro Micronucleus Assay and FISH Analysis -- The In Vivo Rodent Micronucleus Test -- Chromosomal Aberration Test Utilities In Vitro and In Vivo -- Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues -- T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure -- The Human RBC PIG-A Gene Mutation Assay -- The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies -- The Comet Assay: High Throughput Use of FPG -- The Comet Assay In Vivo in Humans -- Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay -- The Comet Assay in Drosophila: Neuroblast and Haemocyte Cells -- The SMART Assays of Drosophila: Wings and Eyes as Target Tissues -- Testing the Genotoxic Potential of Nanomaterials Using Drosophila -- Transgenic Rodent Gene Mutation Assay in Somatic Tissues -- The Mouse Lymphoma Assay -- Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens -- Methods for Measuring DNA Repair: Introduction and Cellular Repair -- A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay -- Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster -- Use of RNA Interference to Study DNA Repair -- The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials
    Abstract: Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the €œÓECD guidelines for the testing of chemicalś€, this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab. ℗ Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study
    Pages: XII, 483 p. 70 illus., 42 illus. in color. : online resource.
    ISBN: 9781493910687
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  • 39
    Keywords: Medicine ; Immunology ; Medical parasitology ; Emerging infectious diseases ; Biomedicine ; Immunology ; Infectious Diseases ; Parasitology ; Springer eBooks
    Description / Table of Contents: The Role of Antibody in Parasitic Helminth Infections -- Maternal Helminth Infections -- Helminth-M. TB Co-Infection -- Bacterial Infections and Vaccines -- Helpful or a℗ Hindrance: Co-Infections of Helminth During Malaria -- Helminth Infections and HIV: Theories and Facts Regarding HIV and Helminth Interaction
    Abstract: Helminth infections are common, cause considerable pathology, and alter a host’s immune profile. This can have important consequences not only on the host’s ability to control a helminth infection, but also on their ability to control unrelated infections. In endemic areas, understanding how helminth infection influences the outcome of common infectious diseases and changes the efficacy of childhood vaccination programs is an important public health question. This book reviews how host immunity to helminths alters our ability to respond to the major pathogens that exist in helminth endemic regions. Current understanding of how helminths alter important but relatively neglected contributors to the host’s anti-helminth immune responses are addressed, namely host antibody responses and how maternal infection may alter a child’s immune development. These are discussed in relation to the control of helminth infection and unrelated infections. Also covered are how helminth infections alter the host’s ability to control TB, HIV and malarial infections along with neglected bacterial infections, such as cholera, and how endemic helminth infections are likely to alter our ability to respond to life-saving vaccination strategies
    Pages: X, 152 p. 6 illus. in color. : online resource.
    ISBN: 9781493914890
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  • 40
    Keywords: Medicine ; Neurosciences ; Toxicology ; Neurology ; Biomedicine ; Neurosciences ; Pharmacology/Toxicology ; Neurology ; Springer eBooks
    Description / Table of Contents: A brief introduction to extrasynaptic GABAA receptors and ‘tonic’ GABAA receptor mediated inhibition in physiology and disease -- Extrasynaptic GABAA receptors: subunit composition, distribution, and regulation -- Biophysical properties of recombinant 2- AND δ- subunit containing GABAA receptors -- The pharmacology of extrasynaptic GABAA receptors -- Neurosteroids and extrasynaptic GABAA receptors -- Sources of GABA that activate extrasynaptic GABAA receptors -- Modulation of Extrasynaptic GABAA Receptors by G-protein-coupled Receptors -- Extrasynaptic GABAA receptors and tonic inhibition in spinal cord -- The role of peri-synaptic GABA receptors after stroke -- The role of extrasynaptic GABAA receptors in focal epilepsy -- Gain-of-Function of Thalamic Extrasynaptic GABA-A Receptors in Typical Absence Seizures -- GABAergic control of the hypothalamic-pituitary-adrenal (HPA) axis: role of extrasynaptic GABAA receptors -- Tonic GABAA receptor mediated inhibition in Fragile-X Syndrome: A cause of dysfunction or a pathway for a cure?
    Abstract: GABA is the principal inhibitory neurotransmitter in the CNS and acts via GABAA and GABAB receptors. Recently, a novel form of GABAA receptor-mediated inhibition, termed “tonic” inhibition, has been described. Whereas synaptic GABAA receptors underlie classical “phasic” GABAA receptor-mediated inhibition (inhibitory postsynaptic currents), tonic GABAA receptor-mediated inhibition results from the activation of extrasynaptic receptors by low concentrations of ambient GABA. Extrasynaptic GABAA receptors are composed of receptor subunits that convey biophysical properties ideally suited to the generation of persistent inhibition and are pharmacologically and functionally distinct from their synaptic counterparts. This book highlights ongoing work examining the properties of recombinant and native extrasynaptic GABAA receptors and their preferential targeting by endogenous and clinically relevant agents. In addition, it emphasizes the important role of extrasynaptic GABAA receptors in GABAergic inhibition throughout the CNS and identifies them as a major player in both physiological and pathophysiological processes
    Pages: IX, 295 p. 34 illus., 18 illus. in color. : online resource.
    ISBN: 9781493914265
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  • 41
    Keywords: Medicine ; Neurosciences ; Cell receptors ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Receptors ; Springer eBooks
    Description / Table of Contents: 1. Introduction: G-protein Signaling in the Retina -- Part One- Phototransduction in Rods and Cones -- 2. G-protein Deactivation Mechanisms in Vertebrate Phototransduction -- 3. Signaling by Rod and Cone Photoreceptors: Opsin Properties, G-protein Assembly and Mechanisms of Activation -- 4. G-protein€“éffector Coupling in the Vertebrate Phototransduction Cascade -- Part II- Inner Retinal GPCR Signaling Pathways -- 5. Interdependence among Members of the mGluR6 G-protein Mediated Signalplex of Retinal Depolarizing Bipolar Cells -- 6. Mechanistic Basis for G-protein Function in ON Bipolar Cells -- 7. Modulation of Trpm1 and the mGluR6 cascade in ON Bipolar Cells -- 8. The Role of Dopamine in Fine-tuning Cone- and Rod-driven Vision -- 9. Regulation of Electrical Synaptic Plasticity in the Retina by G-protein Coupled Receptors -- Part III- Signaling by Photosensitive Ganglion Cells -- 10. The Functional Properties of the G-protein-coupled Receptor melanopsin in Intrinsically Photosensitive Retinal Ganglion Cells
    Abstract: We have learned a great deal about the organization and function of GPCR pathways, and the role that they play in vision. The information gained from these studies has provided, and will continue to provide, critical insights to further our understanding of complex GPCR pathways in the central nervous and endocrine systems. In essence, the retina is the first €˜óptogeneticallý€™-driven circuit with clearly understood physiology. Thus, studying GPCR-driven pathways in the retina will likely guide the interpretation of optogenetic experiments, which are increasingly utilized to study central circuits. Collectively, these signaling pathways allow the retina to represent visual space over a wide range of light intensities and to synchronize its function to the day/night cycle. G protein Signaling Mechanisms in the Retina summarizes our current understanding of the organizational principles of GPCR pathways, using insights derived from the study of the retina. The book highlights several G protein signaling cascades, including phototransduction, ON bipolar cell signaling, dopaminergic pathways, and ipRGC signaling
    Pages: VIII, 198 p. 35 illus., 26 illus. in color. : online resource.
    ISBN: 9781493912186
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  • 42
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    New York, NY : Springer
    Keywords: Life sciences ; Botany ; Plant anatomy ; Plant breeding ; Life sciences ; Plant Breeding/Biotechnology ; Plant Sciences ; Plant Anatomy/Development ; Plant Genetics & Genomics ; Springer eBooks
    Description / Table of Contents: Chloroplast Gene Expression – RNA Synthesis and Processing -- Chloroplast Gene Expression – Translation -- The Chloroplast Genome and Nucleo-Cytosolic Crosstalk -- An Overview of Chloroplast Biogenesis and Development -- Dynamic Architecture of Plant Photosynthetic Membranes -- Plastid Division -- Stromules -- The Apicoplast: A Parasite's Symbiont -- Mechanisms of Chloroplast Protein Import in Plants -- Protein Routing Processes in the Thylakoid -- Protein Transport into Plastids of Secondary Evolved Organisms -- Processing and Degradation of Chloroplast Extension Peptides -- Molecular Chaperone Functions in Plastids -- Plastid Proteases -- Photoprotective Mechanisms: Carotenoids -- Regulation of Electron Transport in Photosynthesis -- Ion Homeostasis in the Chloroplast -- Synthesis of Recombinant Products in the Chloroplast -- Hydrogen and Biofuel Production in the Chloroplast
    Abstract: Plastids are the sites of conversion of solar energy into the chemical energy usable to sustain life. They are also responsible for the production of the vast majority of oxygen in the atmosphere. Through these activities they play a unique role in the biosphere, producing two critical products upon which life on Earth depends. This book provides the scientific community with a critical overview of the state of research in plastids that include those from green and non-green plants and from apicomplexan protists. It covers actively investigated areas of plastid biology, from biosynthesis to function to their uses in biotechnology. Contributed by world renowned plant biologists, as the fifth title in the Advances in Plant Biology book series, Plastid Biology is therefore both timely and unique in scope. Essential topics cover the differentiation and development of different plastid types and internal organization. They are followed by an in depth look at biogenesis and assembly of plastid proteins and protein complexes, and then by the important metabolic functions in plastids. The book ends with two chapters discussing the role of plastid biology in protein expression biotechnology and in hydrogen and biofuel production. It will serve as a reference resource for instructors and researchers for many years to come
    Pages: XII, 589 p. 51 illus., 46 illus. in color. : online resource.
    ISBN: 9781493911363
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  • 43
    Keywords: Life sciences ; Microbiology ; Botany ; Plant anatomy ; Plant Diseases ; Plant physiology ; Plant breeding ; Life sciences ; Plant Sciences ; Plant Pathology ; Microbiology ; Plant Anatomy/Development ; Plant physiology ; Plant Breeding/Biotechnology ; Springer eBooks
    Description / Table of Contents: Fungal Diseases of Groundnut: Control and Future Challenges -- Plant Growth Promoting Rhizobacteria in Crop Protection and Challenges -- Understanding the Mechanism Involved in PGPR Mediated Growth Promotion and Suppression of Biotic and Abiotic Stress in Plants -- Downy Mildew Disease of Pearl Millet and Its Control -- Research on Plant Pathogenic Fungi in the Genomics Era: From Sequence Analysis to Systems Biology -- Pre and Post Harvest Diseases of Potato and Their Management -- Host-Pathogen Interaction, Plant Diseases, Disease Management Strategies and Future Challenges -- Ug99-Future Challenges -- Increased Virulence of Wheat Rusts and the Threat to Global Crop Production -- Fusarium Diseases of Canadian Grain Crops: Impact and Disease Management Strategies -- Pseudomonas fluorescens: A Potential Bio-control Agent for Management of Fungal Diseases of Crop Plants -- Oat Fungal Diseases and the Application of Molecular Marker Technology for Their Control
    Abstract: This volume presents the issues and challenges of crop pathogens and plant protection. Composed of the latest knowledge in plant pathology, the book covers topics such as fungal diseases of the groundnut, plant growth promoting rhizobacteria, plant pathogenic fungi in the genomics era, the increased virulence of wheat rusts, and oat fungal diseases. Written by experienced and internationally recognized scientists in the field, Future Challenges in Crop Protection Against Fungal Pathogens is a concise yet comprehensive resource valuable for both novice as well as experienced plant scientists and researchers
    Pages: XIII, 364 p. 68 illus., 47 illus. in color. : online resource.
    ISBN: 9781493911882
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  • 44
    Keywords: Medicine ; Human Physiology ; Biochemistry ; Biomedicine ; Human Physiology ; Medicine/Public Health, general ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: A20€”Á Bipartite Ubiquitin Editing Enzyme with Immunoregulatory Potential -- The Biology of A20-Binding Inhibitors of NF- ÎðB Activation (Abins) -- The Biology of A20-Like Molecules -- Anti-Viral Tetris: Modulation of the Innate Anti-Viral Immune Response by A20 -- A20 Expressing Tumors and Anticancer Drug Resistance -- Translational Studies of A20 in Atherosclerosis and Cardiovascular Disease -- A Biomarker of Allograft Outcome: A Showcase in Kidney Transplantation -- A20€”Án Omnipotent Protein in the Liver: Prometheus Myth Resolved? -- Emerging Roles for A20 in Islet Biology and Pathology -- Single Nucleotide Polymorphisms at the TNFAIP3/A20 Locus and Susceptibility/Resistance to Inflammatory and Autoimmune Diseases
    Abstract: This book is meant to provide the most current knowledge regarding the mechanisms of action of A20, if its versatile functions and their validation in animal models of human disease and its promise as a diagnostic biomarker and therapeutic target
    Pages: XXI, 188 p. : online resource.
    ISBN: 9781493903986
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  • 45
    Keywords: Life sciences ; Pharmaceutical technology ; Biotechnology ; Plant breeding ; Life sciences ; Plant Breeding/Biotechnology ; Biotechnology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Introduction — Plant-produced protein products -- Commercial Plant-Produced Recombinant Avidin -- Molecular farming in plants – the long road to the market -- TrypZean™: an animal-free alternative to bovine trypsin -- Production of Pharmaceutical Grade Recombinant Native Aprotinin and Non-Oxidized Aprotinin-Variants Under Greenhouse and Field Conditions -- Influenza virus-like particles produced in Nicotiana benthamiana protect against a lethal viral challenge in mice -- Plant-Produced Recombinant Transmission Blocking Vaccine Candidates to Combat Malaria -- An oral vaccine for TGEV immunization of pigs -- Edible Rabies Vaccines -- Newcastle disease vaccines -- An oral vaccine for hepatitis B: challenges, setbacks and breakthroughs -- Commercial Plant-Produced Recombinant Cellulases for Biomass Conversion -- Brazzein: A High Intensity Natural Sweetener -- The Future of Plant-Produced Pharmaceuticals and Industrial Proteins
    Abstract: Attention has recently turned to using plants as hosts for the production of commercially important proteins. The twelve case studies in this volume present successful strategies for using plants to produce industrial and pharmaceutical proteins and vaccine antigens. They examine in detail projects that have commercial potential or products that have already been commercialized, illustrating the advantages that plants offer over bacterial, fungal or animal cell-culture hosts.℗ There are many indications that plant protein production marks the beginning of a new paradigm for the commercial production of proteins that, over the next decade, will expand dramatically
    Pages: XII, 281 p. 33 illus., 17 illus. in color. : online resource.
    ISBN: 9783662438367
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  • 46
    Keywords: Medicine ; Immunology ; Biochemistry ; Cytology ; Developmental Biology ; Biomedicine ; Immunology ; Cell Biology ; Developmental Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Overview of orchestration of CD4+ T cell subsets in immune responses -- Th1/Th2 cell differentiation and molecular signals -- Th1/Th2 cell’s function in immune system -- Regulatory T Cell differentiation and their function in immune regulation -- Th17 differentiation and their pro-inflammation function -- Tfh Cell differentiation and their function in promoting B cell responses -- Differentiation, Regulation and Function of Th9 Cell -- The biology and functions of Th22 cell
    Abstract: This book will focus on the differentiation and regulation of subsets of CD4+ T cells. It will also cover other aspects of research on these cells, which has made great advances in recent years, such as subsets’ plasticity and their role in healthy and disease conditions. The book provides researchers and graduate students with a cutting-edge and comprehensive overview of essential research on CD4+ T cells
    Pages: XI, 230 p. 22 illus., 21 illus. in color. : online resource.
    ISBN: 9789401794879
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  • 47
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Progesterone Receptor Interaction with Chromatin -- Mapping the Genomic Binding Sites of the Activated Retinoid X Receptor in Murine Bone Marrow-Derived Macrophages using Chromatin Immunoprecipitation Sequencing -- Analysis of Chromatin-Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation -- Examining Estrogen Regulation of Cancer Stem Cells through Multicolor Lineage Tracing -- Reporter Mice for the Study of Long Term Effects of Drugs and Toxic Compounds -- Analysis of Histone post-Translational Modifications in the Control of Chromatin Plasticity Observed at Estrogen-Responsive Sites in Human Breast Cancer Cells -- In Silico Analysis of Genomic Data for Construction of Nuclear Receptor Network -- Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment -- Paxillin and Steroid Signaling: from Frog to Human -- Analysis of the Androgen Receptor/Filamin A Complex in Stromal Cells -- Multi-Well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membrane Vs. Intracellular Receptor Levels -- Promixity Ligation℗ Assay to Detect and Localize the Interactions of ERa with PI3-K and Src in Breast Cancer Cells and Tumor Samples -- Phosphoinositide 3-Kinase Assay in Breast Cancer Cell Extracts -- Rapid Estrogen Effects on Aromatase Phosphorylation in Breast Cancer Cells -- Mouse Monoclonal Antibodies against Estrogen Receptor -- Analysis of Estrogen Receptor Interacting Proteins Using Pull Down Assay and MALDI-MS Methods -- Analysis of the Conformation of the Androgen Receptor in Spinal Bulbar Muscular Atrophy by Atomic Force Microscopy -- Imaging of Corticosteroid Receptors in Live Cells -- Physiological Techniques in the Study of Rapid Aldosterone Effects -- Detection of the Glucocorticoid Receptors in Brain Protein Extracts by SDS-PAGE
    Abstract: Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies.℗ Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors
    Pages: XII, 245 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493913466
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  • 48
    Keywords: Medicine ; Genetic Engineering ; Computer Simulation ; Microbial genetics ; Entomology ; Biomedicine ; Biomedicine general ; Microbial Genetics and Genomics ; Genetic Engineering ; Entomology ; Simulation and Modeling ; Springer eBooks
    Description / Table of Contents: Foreword by Michel Morange -- Preface -- 1 What is synthetic biology? -- 2 What was synthetic biology? -- 3 What is life? -- 4 Strategies for making life -- 5 Synthetic biology in action -- 6 The iGEM competition -- 7 Are we doing synthetic biology? Postface by Ricard Solé -- Onomastic index -- Subject index
    Abstract: Synthetic Biology (SB) is a revolutionary discipline with a vast range of practical applications, but is SB research really based on engineering principles? Does it contributing to the artificial synthesis of life or does it utilise approaches sufficiently advanced to fall outside the scope of biotechnology or metabolic engineering? This volume reviews the development of SB and includes the major milestones of the discipline, the ́€˜top-dowń€™ and ́€˜bottom-uṕ€™ approaches towards the construction of an artificial cell and the development of the €œíGEḾ€ competition. We conclude that SB is an emerging field with extraordinary technological potential, but that most research projects actually are an extension of metabolic engineering since the complexity of living organisms, their tight dependence on evolution and our limited knowledge of the interactions between the molecules, actually make life difficult to engineer
    Pages: XII, 77 p. 24 illus., 21 illus. in color. : online resource.
    ISBN: 9789401793827
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  • 49
    Keywords: Medicine ; Pharmaceutical technology ; Biomedicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: 1 Hydrophilic Matrix Dosage Forms: Definitions, General Attributes and the Evolution of Clinical Utilization -- 2 Design and Evaluation of Hydroxypropyl Methylcellulose Matrix Tablets for Oral Controlled Release: a Historical Perspective -- 3 An Industrial Perspective on Hydrophilic Matrix Tablets based on Hyproxypropyl Methylcellulose (Hypromellose) -- 4 Natural Polysaccharides in Hydrophilic Matrices -- 5 Applications of Polyethylene Oxide (POLYOX) in Hydrophilic Matrices -- 6 A Formulation Development Perspective on Critical Interactions Affecting the Performance of Hydrophilic Matrix Tablets -- 7 In vitro Physical and Imaging Techniques to Evaluate Drug Release Mechanisms from Hydrophilic Matrix Tablets -- 8 Physiologically-Based Pharmacokinetic Modelling in the Development and Evaluation of Hydrophilic Matrix Tablets -- 9 Approaches to Rapid In Vivo Optimization of Hydrophilic Matrix Tablets -- 10 Extrusion: an Enabling Technology for Controlled Release Hydrophilic Matrix Systems -- 11 Microenvironmental pH Control and Mixed Polymer Approaches to Optimize Drug Delivery with Hydrophilic Matrix Tablets -- 12 Evolving Biopharmaceutics Perspectives for Hydrophilic Matrix Tablets: Dosage Form-Food Interactions and Dosage Form Gastrointestinal Tract Interactions
    Abstract: This detailed volume addresses key issues and subtle nuances involved in developing hydrophilic matrix tablets as an approach to oral controlled release. It brings together information from more than five decades of research and development on hydrophilic matrix tablets and provides perspective on contemporary issues.Twelve comprehensive chapters explore a variety of topics including polymers (hypromellose, natural polysaccharides and polyethylene oxide) and their utilization in hydrophilic matrices, critical interactions impacting tablet performance, in vitro physical and imaging techniques, and microenvironmental pH control and mixed polymer approaches, among others. In one collective volume, Hydrophilic Matrix Tablets for Oral Controlled Release provides a single source of current knowledge, including sections of previously unpublished data. It is an important resource for industrial and academic scientists investigating and developing these oral controlled release formulations
    Pages: IX, 326 p. 102 illus., 37 illus. in color. : online resource.
    ISBN: 9781493915194
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  • 50
    Keywords: Medicine ; Human Physiology ; Neurosciences