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  • Springer  (117,755)
  • 2010-2014  (2,549)
  • 1970-1974  (115,206)
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  • 1
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    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 2
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 3
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 4
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 5
    Keywords: Life sciences ; Human Genetics ; Animal genetics ; Life sciences ; Animal Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Twenty Years of qPCR: A Mature Technology? -- Minimum Information Necessary for Quantitative Real-Time PCR Experiments -- Selection of Reliable Reference Genes for RT-qPCR Analysis -- Introduction to Digital PCR -- mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling -- Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter -- Absolute Quantification of Viral DNA: The Quest for Perfection -- A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical℗ Samples -- Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of℗ Community-Acquired Pneumonia -- A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition -- Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia -- A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR -- Circulating Cell-Free DNA in Cancer -- Gene Expression Analysis by qPCR in Clinical Kidney Transplantation -- Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data -- Clinical Applications Using Digital PCR -- Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR
    Abstract: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation
    Pages: XI, 231 p. 80 illus., 66 illus. in color. : online resource.
    ISBN: 9781493907335
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  • 6
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Assaying NF-kB Activation and Signaling from TNF Receptors -- Dissecting DR3 Signaling -- Modulation of FceRI-Dependent Mast Cell Response by OX40L -- Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK -- Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies -- β-actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity -- Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model -- Analysis of TNF-Mediated Cerebral Pericyte Remodeling -- CD137 in Chronic Graft-Versus-Host Disease -- In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor-α and Interleukin-1 in Murine Osteoclastogenesis -- Evasion of TNF-α Mediated Apoptosis by Hepatitis C Virus -- TNF-α Modulates TLR2-Dependent Responses During Mycobacterial Infection -- Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label -- A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions -- Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys -- Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing -- Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT -- One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra
    Abstract: Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules.℗ Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis, and osteoclastogenesis
    Pages: XI, 226 p. 43 illus., 15 illus. in color. : online resource.
    ISBN: 9781493906697
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  • 7
    Keywords: Life sciences ; Biochemistry ; Neurobiology ; Microscopy ; Life sciences ; Protein Science ; Biological Microscopy ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells -- Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo -- Optochemical Activation of Kinase Function in Live Cells -- Photoswitching of Cell Surface Receptors Using Tethered Ligands -- Photocontrol of AMPA Receptors with a Photochromic Ligand -- Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy -- Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins -- Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions -- A Roadmap to Applying Optogenetics in Neuroscience -- Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors -- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity -- Structural Basis of Photoswitching in Fluorescent Proteins -- Using Photoactivatable GFP to Track Axonal Transport Kinetics -- In Vivo Cell Tracking Using PhOTO Zebrafish -- In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish℗ Expressing the KillerRed Photosensitizer Protein -- Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy -- pcSOFI as a Smart Label-based Superresolution Microscopy Technique
    Abstract: Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols℗ highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems
    Pages: X, 278 p. 58 illus., 53 illus. in color. : online resource.
    ISBN: 9781493904709
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  • 8
    Keywords: Medicine ; Immunology ; Vaccines ; Genetic Engineering ; Biomedicine ; Immunology ; Genetic Engineering ; Vaccine ; Springer eBooks
    Description / Table of Contents: A Blueprint for DNA Vaccines Design -- Enhancement of Plasmid-Mediated Transgene Expression -- Strategies for Improving DNA Vaccines Performance -- Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies -- Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies -- Development of Antibiotic-Free Selection System for Safer DNA Vaccination -- Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin -- DNA Vaccination in Skin Enhanced by Electroporation -- Intradermal Vaccination by DNA Tattooing -- Microneedle Applications for DNA Vaccine Delivery to the Skin -- Multivalent DNA-Based Vectors for DNA Vaccines Delivery -- Superparamagnetic Nanoparticles Delivery of DNA Vaccine -- Plasmid Fermentation Process for DNA Immunization Applications -- Pharmaceutical Grade℗ Large Scale℗ Plasmid DNA Manufacturing Process -- Protective and Therapeutic DNA Vaccination Against Allergic Diseases -- Immunotherapy for Alzheimeŕ€™s Disease: DNA- and Protein-Based Epitope Vaccines -- Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue -- DNA Vaccination as a Treatment for Chronic Kidney Disease
    Abstract: DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.℗ Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.℗ Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease
    Pages: XI, 308 p. 43 illus., 32 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493904105
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  • 9
    Keywords: Medicine ; Medical laboratories ; Medical virology ; Emerging infectious diseases ; Medicine & Public Health ; Infectious Diseases ; Virology ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
    Abstract: Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research
    Pages: XIII, 403 p. 82 illus., 49 illus. in color. : online resource.
    ISBN: 9781493903481
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  • 10
    Keywords: Medicine ; Neurosciences ; Neurosurgery ; Surgery ; Laboratory animals ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Neurosurgery ; Animal Models ; Surgery ; Springer eBooks
    Description / Table of Contents: Regulatory and Ethical Considerations -- Elements of Descriptive Neuroanatomy -- Elements of Functional Neuroanatomy: The Major Neurotransmitter Systems -- Stereotaxic Approach of a Target Structure -- Preparation of the Stereotaxic Surgical Procedure -- Post-mortem examination of animals
    Abstract: Stereotaxic neurosurgery in rodents is used by a variety of people working at research laboratories (research staff, technicians, students at animal facilities...). The present handbook presents all the steps necessary to complete a stereotaxic neurosurgery protocol in accordance with current animal welfare guidelines. This book will guide surgeons step by step, from anesthesia to the post-surgery recovery procedures, including asepsis of the surgical tools and surgical zone, analgesia, correctly identifying the reference points on the skull and brain targets, etc. In keeping with the current international trends, the authors above all focus on the following points: the consideration of pain and how to best treat it depending on the type of surgery; and ensuring asepsis. This book will serve as an important reference work and valuable guidebook for the scientific community
    Pages: XVIII, 181 p. 151 illus., 136 illus. in color. : online resource.
    ISBN: 9782817804729
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  • 11
    Keywords: Life sciences ; Biochemistry ; Ecology ; Microbiology ; Plant physiology ; Plant breeding ; Environmental sciences ; Life sciences ; Microbiology ; Plant physiology ; Plant Breeding/Biotechnology ; Environment, general ; Ecology ; Protein Science ; Springer eBooks
    Description / Table of Contents: The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress -- The Role of Arbuscular Mycorrhizal Fungi in Alleviation of Salt Stress -- Biological control: PGPR and Arbuscular Mycorrhizal Fungi Working Together -- Role of AM Fungi in Alleviating Drought Stress in Plants -- Mycorrhizal Fungi to Alleviate Salinity Stress on Plant Growth -- Impact of Biotic, Abiotic Stressors: Biotechnologies for Alleviating Plant Stress -- Siderophore Efficacy of Fluorescent Pseudomonades affecting Labeled Iron (59Fe) Uptake by Wheat (Triticum aestivum L.) Genotypes Differing in Fe Efficiency -- Plant Physiological Mechanisms of Salt Tolerance Induced by Mycorrhizal Fungi and Piriformospora indica -- Safflower (Carthamus Tinctorius L.) Oil Content and Yield Components as Affected by Co-Inoculation with Azotobacter Chroococcum and Glomus Intraradices at Various N and P Levels in a Dry Climate -- Mycorrhizal Fungi to Alleviate Compaction Stress on Plant Growth -- Microbial Inoculums
    Abstract: Use of Microbes for the Alleviation of Soil Stresses, Volume 2: Alleviation of Soil Stress by PGPR and Mycorrhizal Fungi describes the most important details and advances related to the alleviation of soil stresses by PGPR and mycorrhizal fungi. Comprised of eleven chapters, the book reviews the role of arbuscular mycorrhizal fungi in alleviation of salt stress, the role of AM fungi in alleviating drought stress in plants, the impact of biotic and abiotic stressors, and the use of mycorrhizal fungi to alleviate compaction stress on plant growth. Written by experts in their respective fields, Use of Microbes for the Alleviation of Soil Stresses, Volume 2: Alleviation of Soil Stress by PGPR and Mycorrhizal Fungi is a comprehensive and valuable resource for researchers and students interested in the field of microbiology and soil stresses
    Pages: X, 192 p. 9 illus. in color. : online resource.
    ISBN: 9781493907212
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  • 12
    Keywords: Medicine ; Human Physiology ; Immunology ; Pneumology ; Biomedicine ; Human Physiology ; Immunology ; Pneumology/Respiratory System ; Springer eBooks
    Description / Table of Contents: Mitochondrial Genetic Abnormalities after Radiation Exposure -- Crediting Six Discoverers of Oxygen -- Hypoxia in Tumors: Pathogenesis-related Classification, Characterization of Hypoxia subtypes, and Associated Biological and Clinical Implications -- Heterogeneity in Tissue Oxygenation: From Physiological Variability in Normal Tissues to Pathophysiological Chaos in Malignant Tumours -- Oxygen Diffusion: An Enzyme-controlled Variable Parameter -- Role of Microvascular Shunts in the Loss of Cerebral Blood Flow Autoregulation -- Impact of Hypoxia-related Tumor Acidosis on Cytotoxicity of Different Chemotherapeutic Drugs in vitro and in vivo -- The Founding of ISOTT: The Shamattawa of Engineering Science and Medical Science -- A Tale of Two Methods: Combining Near-Infrared Spectroscopy with MRI for Studies of Brain Oxygenation and Metabolism -- Advances in Probes and Methods for Clinical EPR Oximetry -- Real-Time, In Vivo Determination of Dynamic Changes in Lung and Heart Tissue Oxygenation using EPR Oximetry -- Modulation of Hypoxia by Magnetic Nanoparticle Hyperthermia to Augment Therapeutic Index -- Skeletal Muscle and Glioma Oxygenation by Carbogen Inhalation in Rats: A Longitudinal Study by EPR Oximetry using Single-Probe Implantable Oxygen Sensors -- Recurrent Low-dose Chemotherapy to Inhibit and Oxygenate Head and Neck Tumors -- How in vivo EPR Measures and Images Oxygen -- What We Learn From In Vivo EPR Oxygen Images -- EPR Image Based Oxygen Movies for Transient Hypoxia -- Repetitive Measurements of Intrarenal Oxygenation in vivo using L band Electron Paramagnetic Resonance -- Quantitative Hypoxia Imaging for Treatment Planning of Radiotherapy -- A New Flavonoid Regulates Angiogenesis and Reactive Oxygen Species Production -- Angiotensin II Reduces Transport-Dependent Oxygen Consumption but Increases Transport-Independent Oxygen Consumption in Immortalized Mouse Proximal Tubular Cells -- Investigation of Cerebral Autoregulation in the Newborn Piglet during Anaesthesia and Surgery -- Influence of the Maternal use of Labetalol on the Neurogenic Mechanism for Cerebral Autoregulation Assessed by Means of NIRS -- Development of a Near Infrared Multi-Wavelength, Multi-channel, Time-Resolved Spectrometer for Measuring Brain Tissue Haemodynamics and Metabolism -- Simulating NIRS and MRS measurements during cerebral hypoxia-ischaemia in piglets using a computational model -- Analysis of Slow Wave Oscillations in Cerebral Haemodynamics and Metabolism Following Subarachnoid Haemorrhage -- Effects of Enriched Environment on Hippocampal Neuronal Cell Death and Neurogenesis in Rat Global Ischemia -- Automated Image Analysis for Diameters and Branching Points of Cerebral Penetrating Arteries and Veins Captured with Two-Photon Microscopy -- Cerebral Hemodynamic Changes and Metabolic Alteration in Severe Hemorrhagic Shock -- Physiological Mechanism of Increase in Deoxy-hemoglobin Concentration during Neuronal Activation in Patients with Cerebral Ischemia: A Simulation Study with the Balloon Model -- Effect of Blood in the Cerebrospinal Fluid on the Accuracy of Cerebral Oxygenation Measured by Near Infrared Spectroscopy -- Vessel Specific Imaging of Glucose Transfer with Fluorescent Glucose Analogue in Anesthetized Mouse Cortex -- Ischemic Pretreatment Delays Ischemic Brain Vasospasm Injury in Gerbils -- Changes in Cerebral Blood Oxygenation Induced by Active Standing Test in Children with POTS and NMS -- Optical Imaging of Brain Activation in Gambian Infants -- Asymmetrical Changes in Cerebral Blood Oxygenation Induced by an Active Standing Test in Children with Postural Tachycardia Syndrome -- Changes of Cerebral Tissue Oxygen Saturation at Sleep Transitions in Adolescents -- Influence of Subjective Happiness on the Prefrontal Brain Activity: An fNIRS Study -- Ginkobiloba Extract Improves Working Memory Performance in Middle-aged women; Role of Asymmetry of Prefrontal Cortex Activity during a Working Memory Task -- Bayesian Prediction of Anxiety Level in Aged People at Rest using 2-Channel NIRS Data from Prefrontal Cortex -- Short-term Hypoxic Preconditioning Improved Survival following Cardiac Arrest and Resuscitation in rats -- Venular Valves and Retrograde Perfusion -- Monitoring of Filter Patency during Carotid Artery Stenting using Near-Infrared Spectroscopy with High Time-Resolution -- Use of NIRS to Assess Effect of Training on Peripheral Muscle Oxygenation Changes in Elite Rugby Players Performing Repeated Supramaximal Cycling Tests -- Skeletal Muscle Deoxygenation Responses during Treadmill Exercise in Children -- Development of a Hybrid Microwave-Optical Thermoregulation Monitor for the Muscle -- Evaluation of a Textile-Based Near Infrared Spectroscopy System in Calf Muscle Oxygenation Measurements -- Skin Temperature in Lower Hind Limb Subjected to Distal Vein Arterialization in Rats -- Index
    Abstract: This book contains the refereed contributions from the 41st annual meeting of ISOTT. The annual meetings of ISOTT bring together scientists from various fields (medicine, physiology, mathematics, biology, chemistry, physics, engineering, etc.) in a unique international forum. Traditionally, ISOTT conferences are a place, where an atmosphere of interaction is created, where many questions are asked after each presentation and lively discussions occur at a high scientific level. This vivid interaction is the main motivation for members to participate and gain new ideas and knowledge in the broad field of oxygen transport to tissue. The papers in this volume summarize some of the outstanding contributions from the 41st annual meeting. Special features in this volume include invited presentations from senior members of ISOTT for the theme “the wisdom of ISOTT” in which founders, past presidents and prize winners from previous meetings provided both cutting edge new knowledge and integrated overviews of critical aspects of the field. The presentations and manuscripts also include those provided by the special opportunity provided by having part of the ISOTT meeting overlap with the EPR-2013 meeting where both focused on preclinical and clinical measurements of oxygen, with a particular emphasis on cancer
    Pages: XXX, 372 p. 152 illus., 60 illus. in color. : online resource.
    ISBN: 9781493906208
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  • 13
    Keywords: Life sciences ; Proteomics ; Biochemistry ; Life sciences ; Protein Science ; Proteomics ; Springer eBooks
    Description / Table of Contents: Survey of Shotgun Proteomics -- LC-MALDI-TOF/TOF for Shotgun Proteomics -- Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry -- GeLC-MS/MS Analysis of Complex Protein Mixtures -- IPG Strip-Based Peptide Fractionation for Shotgun Proteomics -- SILAC Yeast: From Labeling to Comprehensive Proteome Quantification -- Analysis of Proteome Dynamics in Mice by Isotopic Labeling -- Stable Isotope Labeling in Mammals (SILAM) -- Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale -- Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification -- Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL) -- Employing TMT Quantification in a Shotgun-MS Platform -- Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle -- Spectral Counting Label-Free Proteomics -- Quantification of Proteins by Label Free LC-MSE -- Bioinformatics for Proteomics: Opportunities at the Interface Between the Scientists, Their Experiments and the Community -- Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry -- Application of Shotgun Proteomics for Discovery-Driven Protein-Protein Interaction -- Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags -- Biomarker Verification Using Selected Reaction Monitoring and Shotgun Proteomics -- Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers -- The Secretome Analysis by High Throughput Proteomics and Multiple Reaction Monitoring (MRM) -- Preparation of Heteroelement-Incorporated and Stable Isotope-Labeled Protein Standards for Quantitative Proteomics -- One-Source Peptide/Phosphopeptide Ratio Standards for Accurate and Site-Specific Determination of the Degree of Phosphorylation -- Quantitative Glycoproteomics for N-Glycoproteome Profiling -- A Practical Recipe to Survey Phosphoproteomes -- Quantitation of the Phosphoproteome Using the Library Assisted eXtracted Ion Chromatogram (LAXIC) Strategy -- Fast, Efficient and Quality-Controlled Phosphopeptide Enrichment from Minute Sample Amounts Using Titanium Dioxide -- Quantifying Small Molecule-Induced Changes in Cellular Protein Expression and Post-Translational Modifications Using Isobaric Mass Tags -- Analysis of Protein Structure by Crosslinking Combined with Mass Spectrometry -- Top Down Proteomics by Means of Orbitrap Mass Spectrometry
    Abstract: Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.℗ Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species
    Pages: XIV, 492 p. 81 illus., 48 illus. in color. : online resource.
    ISBN: 9781493906857
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  • 14
    Keywords: Life sciences ; Geography ; Regional planning ; Ecology ; Nature conservation ; Life sciences ; Ecology ; Geography (general) ; Landscape/Regional and Urban Planning ; Geoecology/Natural Processes ; Nature conservation ; Springer eBooks
    Description / Table of Contents: 1. Introduction 2. Oceanic Types and Their Controls -- 3. The Ecoregions of the Oceans -- 4. Continental Types and Their Controls -- 5. Ecoregions of the Continents: The Polar Ecoregions -- 6. The Humid Temperate Ecoregions -- 7. The Dry Ecoregions -- 8. The Humid Tropical Ecoregions -- 9. The Mountain Ecoregions -- 10. Ecoregion Redistribution under Climate Change -- 11. Continental Patterns and Boundaries -- 12. Application of Ecoregional Patterns -- 13. Fire Regimes at the Ecoregion Scale -- 14. Summary and Conclusions -- Appendix A: Air Masses and Frontal Zones -- Appendix B: Common and Scientific Names -- Appendix C. Conversion Factors -- Appendix D. Comparison of Ecoregion and Related Approaches
    Abstract: The first edition of this book (1998) classified and characterized the regional-scale ecosystem units (ecoregions) of the Earth as shown on a map that Bailey developed with the encouragement of several international organizations. In addition to the descriptive account, his primary goal was to suggest explanations of the mechanisms that act to produce the world pattern of ecoregion distribution, and to consider some of the implications for land use. He included ocean types, since understanding land regions depends on understanding ocean systems. The increasing importance of ecoregions is confirmed by the fact that much planning, research, and management efforts by the US Forest Service, The Nature Conservancy, World Wildlife Fund and other organizations are taking place now within the framework of ecoregions. Over years since the book was first published a number of studies have greatly contributed to a better understanding of the Earth's ecoregions. This second edition is a completely updated and expanded version. New sections address how ecoregions are changing under the relentless influence of humans (such as modification of fire regimes and the introduction of invasive species) and climate change, the use of ecoregional patterns to transfer research results and select sites for detecting climate change effects on ecosystem distribution, and the use of ecoregional patterns to design monitoring networks and sustainable landscapes. From the reviews of the 1st edition: "Ecoregions offers an invaluable source of description, interpretation and analysis of global patterns of ecosystem distribution and successfully provides the reader with a means of making sense of these patterns." Geography - this volume provides two major advances. Over previous works Bailey realizes that understanding continental systems requires a grasp of the enormous influence that ocean systems and mountain building exert on terrestrial climatic patterns. The Quarterly Review of Biology. About the Author: Robert G. Bailey is a geographer with the United States Forest Service, Rocky Mountain Research Station, Inventory and Monitoring Program, in Fort Collins, Colorado, and the former leader of the agency's Ecosystem Management Analysis Center. His work involves the application of ecosystem geography to ecological planning and design
    Pages: XV, 180 p. 134 illus., 54 illus. in color. : online resource.
    Edition: 2nd ed.
    ISBN: 9781493905249
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  • 15
    Keywords: Life sciences ; Microbiology ; Bacteriology ; Life sciences ; Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Gene Transfer: Transduction -- Gene Transfer: Transformation/Electroporation -- Gene transfer: Conjugation -- Pseudomonas Bacteriophage Isolation and Production -- Genotyping Methods -- Drug Susceptibility Testing by Dilution Methods -- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa -- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa -- Motility Assay: Twitching Motility -- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis -- Microscopic Analysis: Morphotypes and Cellular Appendages -- Determination of Lipolytic Activities -- Elastinolytic and proteolytic enzymes -- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins -- Cell Fractionation -- Characterization of Molecular Interactions using Isothermal Titration Calorimetry -- Proteomic Analysis -- Membrane Proteomics of Pseudomonas aeruginosa -- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays -- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules -- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules -- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP -- Metabolic Footprinting: Extracellular Metabolomic Analysis -- Pyoverdine and Pyochelin Measurements -- Measurement of Phenazines in Bacterial Cultures -- Extraction and Measurement of NAD(P)+ and NAD(P)H -- Cyanide Measurements in Bacterial Culture and Sputum -- Monitoring Iron Uptake by Siderophores -- Exopolysaccharide Quantification -- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids -- LPS quantitation Procedures -- Monitoring Lectin Interaction with Carbohydrates -- Mining the Pseudomonas Genome -- Identification of Bacterial Small RNAs by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis -- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics -- Signature Tagged Mutagenesis -- Construction of a Pseudomonas aeruginosa Genomic Bank -- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes -- Promoter Fusions with Optical Outputs in Individual Cells and in Populations -- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq -- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans -- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop -- Biofilm Formation in the 96-well Microtiter Plate -- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa -- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans -- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum -- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella -- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana -- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells -- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish -- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster -- Assessing Pseudomonas Virulence using Host Cells -- Assessing Pseudomonas aeruginosa℗ Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection -- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model -- Burn Mouse Models
    Abstract: In℗ Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings
    Pages: XVIII, 816 p. 157 illus., 89 illus. in color. : online resource.
    ISBN: 9781493904730
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  • 16
    Keywords: Life sciences ; Animal behavior ; Animal ecology ; Conservation biology ; Evolution (Biology) ; Life sciences ; Evolutionary Biology ; Animal ecology ; Conservation Biology/Ecology ; Behavioural Sciences ; Springer eBooks
    Description / Table of Contents: Part I: Introduction -- Chapter 1. Introduction: Studying Woolly Monkeys -- Part II: Systematics and Taxonomy -- Chapter 2. Coat Color is not an Indicator of Subspecies Identity in Colombian Woolly Monkeys -- Chapter 3. Colombian Lagothrix: Analysis of their Phenotypes and Taxonomy -- Part III: Ex Situ Research -- Chapter 4. Behavior and Husbandry of a Captive Group of Woolly Monkeys: A Case Study -- Chapter 5. Clinical Experience and Diseases of the Woolly Monkey -- Chapter 6. Recent Advances in Woolly Monkey Nutrition -- Chapter 7. Effects of Housing Conditions and Diet on the Behavior of Captive Woolly Monkeys -- Part IV. In Situ Ecology and Behavior -- Chapter 8. Life History, Behavior and Development of Wild Immature Lowland Woolly Monkeys (Lagothrix poeppigii) in Amazonian Ecuador -- Chapter 9. Seed Dispersal by Woolly Monkeys (Lagothrix lagothricha) at Caparu Biological Station, Colombia: Quantitative Description and Qualitative Analysis -- Chapter 10. Ranging Behavior, Daily Path Lengths, Diet and Habitat USe of Yellow Tailed Woolly Monkeys (Oreonax flavicauda) at La Esperanza, Peru -- Chapter 11 -- Vocal Communication in Woolly Monkeys (Lagothrix lagotricha lugens) at Cueva de los Guacharos National Park, Colombia -- Chapter 12. Potential Determinants of the Abundance of Wolly Monkeys in Neotropical Forests -- Chapter 13. Behavior Ecology and Inter-individual Distance of Woolly Monkeys (Lagothrix lagothricha) in Rainforest Fragment in Colombia -- Part V. Conservation -- 14. Notes on the Behavior of Captive and Released Woolly Monkeys (Lagothrix lagothricha): Reintroduction as a Conservation Strategy in Colombian Southern Amazon -- 15. Population Viability Analysis of Woolly Monkeys in Western Amazonia -- 16. Yellow Tailed Woolly Monkey (Oreonax flavicauda): Conservation Status, Anthropogenic Threats and Conservation Initiatives
    Abstract: Woolly monkeys are large, attractive and widespread primates found throughout many parts of the Amazon basin. It is only in the last twenty-five years or so that long-term studies of woollies℗ in their forest habitat have been successful; they have not generally been successfully kept in captivity. But now, especially because of their size, these creatures are pressed on all sides by bush meat hunters and forest fragmentation. Their future is becoming critically precarious, and the editors feel that it is time to showcase these animals with a full book. The editors draw together a number of recent woolly monkey studies from three Amazonian countries, including five taxa of woolly monkeys, four of which have recently been reclassified without using new biological criteria as species rather than subspecies (Groves, 2001, 2005; Rylands & Mittermeier, 2009). This volume provides a diversity of studies by well-known researchers and advanced students on a wide range of subjects using newly generated data, including a criticism of the recent taxonomic changes. The varied information contained within The Woolly Monkey: Behavior, Ecology, Systematics, and Captive℗ Research℗ will help readers understand these handsome animals and will, we hope,℗ energize them to contribute to their conservation. ℗ Thomas R. Defler, Ph.D., a native English speaker but adopted Colombian, has studied primates for forty years, six years in the United States and thirty-four years in the field in Colombia. Most of his years in Colombia have been spent in eastern Colombian wilderness until his move to the National University campus in Bogot©Ł seven years ago. He founded two research stations in the Colombian Amazon, both of which are still functioning, and he has trained many young Colombians in field techniques. Defler has 112 publications, including℗ three authored℗ books, one edited book and has just finished writing two more yet-unpublished books. Pablo Stevenson, Ph.D., a Colombian native, has studied primates in the field for twenty-two years. Although he has investigated many species of primates, he is by far the most experienced primatologist in the world for research on Lagothrix, which he first began to study in 1988. Stevenson has over 100 publications, many of which involve or showcase Lagothrix. Pablo has trained many young Colombian primatologists as professor at Los Andes University. He has also published a book on the fruits of the R©Ưo Duda region, as well as edited two books on Colombian primates
    Pages: X, 302 p. 70 illus., 33 illus. in color. : online resource.
    ISBN: 9781493906970
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  • 17
    Keywords: Life sciences ; Pediatrics ; Cytology ; Life sciences ; Oxidative Stress ; Pediatrics ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Oxygen in health and disease -- Reactive oxygen species and nitric oxide in vascular function -- Nitrite pathway: Another important nitric oxide production system -- Nitric oxide and beyond: New insights and therapies for endothelial targeting -- Multifunctional roles of nitric oxide in neurons -- Oxidative stress biomarkers: Future perspectives -- Urinary clinical biomarkers in critical care -- Rapid diagnostic tests for oxidative stress status -- Analytical procedures for nitrative/nitrosative stress -- Animal models of enhanced oxidative stress -- Antioxidant supplementation and therapies -- Edaravone therapy -- Melatonin therapy -- Thioredoxin therapy -- Allergic and immunological disorders -- Cardiac disorders -- Environmental pollution and health consequences -- Gastrointestinal disorders -- Genetic disorders -- Growth, puberty and nutritional disturbances -- Hematologic disorders -- Infectious and inflammatory disorders -- Metabolic disorders -- Neurological disorders -- Renal disorders -- Respiratory disorders -- Trauma -- Vascular disorders
    Abstract: This volume examines the role of oxidative stress in the pathology of numerous pediatric disorders. It covers a wide range of topics including the chemistry and biology of reactive oxygen species and nitric oxide, molecular biology of the enzymes generating these species, clinically useful biomarkers for evaluating oxidative stress status in humans, and the pathophysiology, clinical course and management of a variety of pediatric disorders, among others. The book also describes current diagnostic tools, laboratory methods and technology for treatment and prevention of pediatric disorders.℗ It explores emerging technology and medical applications including discussions of biomarkers and antioxidants as therapeutic agents. Written by an international team of experts in both pediatrics and℗ free radical and antioxidant research, Studies on Pediatric Disorders is an invaluable addition to the Oxidative Stress in Applied Basic Research and Clinical Practice series
    Pages: XII, 494 p. 115 illus., 53 illus. in color. : online resource.
    ISBN: 9781493906796
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  • 18
    Keywords: Life sciences ; Microbiology ; Emerging infectious diseases ; Life sciences ; Microbiology ; Medical Microbiology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Part I: Medical Biofilms -- Biofilms in Disease -- The Use of DNA Methods to Characterize Biofilm Infection -- Imaging Biofilms in Tissue Specimens -- Mechanisms of Drug Resistance in Fungi and Their Significance in Biofilms -- Horizontal Gene Transfer in Planktonic and Biofilm Modes -- The Role of Quorum Sensing in Biofilm Development -- Part II: Strategies for Biofilm Control -- Current and Emergent Control Strategies for Medical Biofilms -- The Effect of Plasmids and Other Biomolecules on the Effectiveness of Antibiofilm Agents -- Antimicrobial℗ Coatings to Prevent℗ Biofilm℗ Formation on Medical Devices -- Medicinal Plants and Phytocompounds: A Potential Source of Novel Antibiofilm Agents -- Staphylococcus Aureus Biofilm Formation and Inhibition -- Novel Targets for Treatment Of Pseudomonas Aeruginosa Biofilms -- Inhibition of Fungal Biofilms -- Biofilm Control Strategies in Dental Health -- Inhibition of Polymicrobial Biofilms: Recent Trends -- Antibiofilm Strategies in the Food Industry -- Part III: The Future of Antibiofilm Agents -- Biofilm Inhibition by Nanoparticles -- Drug Delivery Systems That Eradicate and/or Prevent Biofilm Formation -- Eradication of Wound Biofilms by Electrical Stimulation -- The Effects of Photodynamic Therapy in Oral Biofilms -- Clinical and Regulatory Development of Antibiofilm Drugs: The Need, The Potential and The Challenges
    Abstract: This book provides a survey of recent advances in the development of antibiofilm agents for clinical and environmental applications. The fact that microbes exist in structured communities called biofilms has slowly become accepted within the medical community. We now know that over 80% of all infectious diseases are biofilm-related; however, significant challenges still lie in our ability to diagnose and treat these extremely recalcitrant infections. Written by experts from around the globe, this book offers a valuable resource for medical professionals seeking to treat biofilm-related disease, academic and industry researchers interested in drug discovery, and instructors who teach courses on microbial pathogenesis and medical microbiology
    Pages: IX, 497 p. 80 illus., 35 illus. in color. : online resource.
    ISBN: 9783642538339
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  • 19
    Keywords: Medicine ; Oncology ; Immunology ; Biomedicine ; Cancer Research ; Immunology ; Oncology ; Springer eBooks
    Description / Table of Contents: Inflammation and Lung Cancer -- Inflammation and Colon Cancer -- Inflammation and Breast Cancer -- Inflammation and Brain Cancer -- Inflammation and Head & Neck Cancer -- Inflammation and Pancreatic Cancer -- Inflammation and Prostate Cancer -- Inflammation and Bladder Cancer -- Inflammation and Renal Cancer -- Inflammation and Gastric Cancer -- Inflammation and Sarcoma -- Inflammation and Lymphoma -- Inflammation and Leukemia -- Inflammation and Multiple myeloma -- Inflammation and Cervical Cancer -- Inflammation and Liver Cancer -- Inflammation and Skin Cancer
    Abstract: This volume examines in detail the role of chronic inflammatory processes in the development of several types of cancer. Leading experts describe the latest results of molecular and cellular research on infection, cancer-related inflammation, and tumorigenesis. Further, the clinical significance of these findings in preventing cancer progression and approaches to treating the diseases are discussed. Individual chapters cover cancer of the lung, colon, breast, brain, head and neck, pancreas, prostate, bladder, kidney, liver, cervix, and skin as well as gastric cancer, sarcoma, lymphoma, leukemia, and multiple myeloma
    Pages: XII, 490 p. 24 illus., 19 illus. in color. : online resource.
    ISBN: 9783034808378
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  • 20
    Keywords: Life sciences ; Ecology ; Biodiversity ; Plant Ecology ; Conservation biology ; Botany ; Life sciences ; Plant Sciences ; Ecology ; Plant Ecology ; Biodiversity ; Conservation Biology/Ecology ; Springer eBooks
    Description / Table of Contents: Introduction -- 1. Mycorrhizas: An Overview -- 2. Mycorrhizas: Global Patterns and Trends -- 3. Mycorrhizas and Global Climate Change -- 4. Mycorrhizas in Relation to Plant Rarity and Invasiveness -- 5. Mycorrhizas and Ecological Restoration -- 6. Mycorrhizas in Extreme Environments -- 7. Mycorrhizas in Aquatic Plants -- 8. Approaches to Mycorrhizal Studies -- Glossary -- Index -- Bibliography. ℗ ℗
    Abstract: The book provides basic knowledge in mycorrhizal ecology, knitted with novel conceptual frameworks and contemporary perspectives, especially in the context of global change. In a fast changing world wherein anthropogenic climate change, biological invasions, deforestation, desertiƠ̐cation, and frequent droughts have become routine hard realities, the contents of this book urge readers to rethink basic notions of setting and accomplishing objectives in mycorrhizal research to make sense vis-© -vis contemporary challenges. In this book, a global perspective of mycorrhizal diversity and distribution is provided, followed by some insights into the impact of various global change elements such as climate change, plant invasion, and extreme environmental conditions on mycorrhizas and the role of these mutualists in turn to help their host plants to withstand such novel selection pressures. Special attention here is given to the interesting, but largely neglected, topics such as the role of mycorrhizas in ecological restoration of degraded environments and mycorrhizal status of aquatic plants. The basic idea is to unify various topical areas in mycorrhizal science in an integrated framework. This book can be used by the undergraduate and graduate level students studying mycorrhizal symbioses in the context of current ecological applications. The materials in this book will beneƠ̐t biological scientists actively involved in research on mycorrhizal ecology and global environmental change. Besides, the contents of the book could be of special interest to restoration ecologists and biodiversity managers
    Pages: XI, 87 p. 26 illus., 9 illus. in color. : online resource.
    ISBN: 9788132218654
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  • 21
    Call number: QP514:77(9)a ; QP514:77(9) ; M019:39
    Keywords: Biochemistry ; Molecular Biology
    Pages: xxix, 1073 p. : ill.
    Edition: 9., vollständig überarb. Auflage
    ISBN: 9783642179716
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    QP514:77(9)a available
    QP514:77(9) on loan
    M019:39 departmental collection or stack – please contact the library
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  • 22
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Protein Science ; Protein Structure ; Springer eBooks
    Description / Table of Contents: 1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins -- Affinity Purification of Heme-Tagged Proteins -- Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag -- Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag -- Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag -- SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1 -- Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag -- Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins -- Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2 -- Detection of Protein-Protein Interactions Using Tandem Affinity Purification -- An Improved in vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells -- Purification of Recombinant Proteins with a Multifunctional GFP Tag -- Targeted Purification of SnAvi-Tagged Proteins -- An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells -- Bimolecular Affinity Purification - A Variation of TAP with Multiple Applications
    Abstract: Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given proteiń€™s function and structure. Todaý€™s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to ́€œfish out́€ a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information ́€“ information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of todaý€™s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest
    Pages: X, 213 p. 46 illus., 31 illus. in color. : online resource.
    ISBN: 9781493910342
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  • 23
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Targeted Small Non-coding RNA Directed Gene Activation in Human Cells -- Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs -- Generation of Endo-siRNAs in Xenopus laevis Oocytes -- Analysis of Endo-siRNAs in Drosophila -- Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster -- Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans -- Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs -- Extraction and Non-radioactive Detection of Small RNA Molecules -- p19 Mediated Enrichment and Detection of siRNAs -- Detection of Small non-coding RNAs by in situ Hybridization using Probes of 2’-O-Methyl RNA + LNA -- Enhanced Detection of Small RNAs using a non-Radioactive Approach -- Computing siRNA and piRNA Overlap Signatures -- Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference -- Computational Analysis, Biochemical Purification and Detection of tRNA-derived Small RNA Fragments -- Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons
    Abstract: Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.℗ These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals. ℗
    Pages: X, 182 p. 36 illus., 24 illus. in color. : online resource.
    ISBN: 9781493909315
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  • 24
    Keywords: Life sciences ; Botany ; Life sciences ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling -- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR -- Analysis of MAPK Activities using MAPK-specific Antibodies -- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels -- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™ -- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae -- Virus-induced Gene Silencing in Plant MAPK Research -- RNA Interference of Plant MAPK Cascades for Functional Studies -- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes -- Immunofluorescent Localization of MAPKs in Steedman’s Wax Sections -- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies -- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases -- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1 -- In vivo Phosphorylation of WRKY Transcription Factor by MAPK -- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates -- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome -- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays
    Abstract: Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students
    Pages: XI, 266 p. 33 illus., 17 illus. in color. : online resource.
    ISBN: 9781493909223
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  • 25
    facet.materialart.
    London : Springer
    Call number: QZ208:149(4)
    Keywords: Cancer / Genetic aspects ; Neoplasms / genetics ; Neoplastic Syndromes, Hereditary
    Edition: 4th ed.
    ISBN: 9781447123743
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  • 26
    Keywords: Medicine ; Microbiology ; Vaccines ; Emerging infectious diseases ; Epidemiology ; Biomedicine ; Medical Microbiology ; Infectious Diseases ; Vaccine ; Epidemiology ; Springer eBooks
    Description / Table of Contents: Cholera Outbreaks in the Classical Biotype Era -- Cholera Outbreaks in the El Tor Biotype Era and the Impact of the New El Tor variants -- Cholera Outbreaks in India -- Cholera Outbreaks in South East Asia -- Cholera Outbreaks in Africa -- The Cholera Outbreak in Haiti: Where and How Did it Begin?- Role of Phages in the Epidemiology of Cholera -- Circulation and Transmission of Clones of Vibrio Cholerae During Cholera Outbreaks -- Modelling Cholera Outbreaks -- Genomic Science in Understanding Cholera Outbreaks and Evolution of Vibrio cholerae as a Human pathogen -- When, How and Where Can Oral Cholera Vaccines be Used to Interrupt Cholera Outbreaks?
    Abstract: The most feared attribute of the human pathogen Vibrio cholerae is its ability to cause outbreaks that spread like wildfire, completely overwhelming public health systems and causing widespread suffering and death. This volume starts with a description of the contrasting patterns of outbreaks caused by the classical and El Tor biotypes of V. cholerae. Subsequent chapters examine cholera outbreaks in detail, including possible sources of infection and molecular epidemiology on three different continents, the emergence of new clones through the bactericidal selection process of lytic cholera phages, the circulation and transmission of clones of the pathogen during outbreaks, and novel approaches to modeling cholera outbreaks. A further contribution deals with the application of the genomic sciences to trace the spread of cholera epidemics and how this information can be used to control cholera outbreaks. The book closes with an analysis of the potential use of killed oral cholera vaccines to stop the spread of cholera outbreaks
    Pages: X, 263 p. 25 illus., 21 illus. in color. : online resource.
    ISBN: 9783642554049
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  • 27
    Keywords: Life sciences ; Botany ; Plant breeding ; Life sciences ; Plant Genetics & Genomics ; Plant Breeding/Biotechnology ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Principles of Plant-based Vaccines -- Mucosal Immunology and Oral Vaccination -- Viral Vector-based Expression Strategies -- Modalities for Expression of Antigens in Plants: Plastid-based Expression Strategies -- Seed-based Expression Strategies -- Biorreactors for Plant Biomass Production and Bioprocessing -- Influenza -- Neglected Tropical Diseases -- Human Immunodeficiency Virus -- Plant-based Vaccines against Hepatitis B -- Overview of Plant-made Vaccine Antigens against Toxoplasmosis -- Allergen-specific Immunotherapy Using Seed-based Allergy Vaccines Against Pollen Allergens -- Plant-based Vaccines as a Global Vaccination Approach: Current Perspectives
    Abstract: Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View provides an integrated℗ outlook of the disciplines involved in the development of plant-based vaccines as well as an updated compilation of the successful developments in the field. The volume covers immunological aspects of mucosal vaccine design, molecular approaches to attain high levels of the recombinant antigens, the rationale of using bioreactor to expand plant biomass, and pharmaceutical technology approaches that have been applied to the development of plant-based vaccine formulations. Practical figures and tables are presented to facilitate reading and identification of key points. Perspectives for this field are also discussed. Written by authorities in the field, Genetically Engineered Plants as a Source of℗ Vaccines Against Wide Spread Diseases: An Integrated View is a comprehensive resource for researchers and students interested in plant genetics and breeding, immunology, and genetic engineering
    Pages: XII, 287 p. 60 illus., 41 illus. in color. : online resource.
    ISBN: 9781493908509
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  • 28
    Keywords: Life sciences ; Cytology ; Stem Cells ; Cell Culture ; Life sciences ; Cell Biology ; Stem Cells ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage -- Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells -- Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds -- Growth and Differentiation of HaCaT Keratinocytes -- Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors -- Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq -- Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis -- Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline -- Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity -- Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes -- MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures -- Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope -- Modified Methods for Growing 3-D Skin Equivalents: An Update -- A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro -- Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter -- Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications -- Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection -- Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle -- Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies -- Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles -- Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process
    Abstract: Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study
    Pages: XI, 296 p. 150 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493912247
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  • 29
    Keywords: Medicine ; Immunology ; Cell Culture ; Biomedicine ; Immunology ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells -- Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice -- Purification and Immunophenotypic Characterization of Murine B10 B Cells -- Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions -- IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques -- TGF-β Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques -- Characterization and Activity of Fas Ligand Producing CD5+ B Cells -- Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties -- Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines -- Toll-Like Receptor Ligation for the Induction of Regulatory B Cells -- The Generation of Regulatory B Cells by Helminth Parasites -- Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions -- Regulatory B Cells in Experimental Mouse Models of Arthritis -- Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE) -- Regulatory B Cells in Allergic Airways Disease and Asthma -- Regulatory B Cells in Mouse Models of Intestinal Inflammation -- Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE) -- Regulatory B Cells, Helminths and Multiple Sclerosis -- Generation and Identification of Tumor-Evoked Regulatory B Cells
    Abstract: Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology
    Pages: XI, 292 p. 53 illus., 19 illus. in color. : online resource.
    ISBN: 9781493911615
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  • 30
    Keywords: Life sciences ; Proteomics ; Biochemistry ; Life sciences ; Protein Science ; Proteomics ; Springer eBooks
    Description / Table of Contents: Twelve Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) -- Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture -- SILAC Labeling of Yeast for the Study of Membrane Protein Complexes -- Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry -- Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons -- SILAC and Alternatives in Studying Cellular Proteomes of Plants -- In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster -- Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse -- Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans -- SILAC-based Temporal Phosphoproteomics -- Global Ubiquitination Analysis by SILAC in Mammalian Cells -- Quantifying In Vivo, Site-specific Changes in Protein Methylation with SILAC -- Applying SILAC for the Differential Analysis of Protein Complexes -- Defining Dynamic Protein Interactions Using SILAC-based Quantitative Mass Spectrometry -- Identifying Nuclear Protein-Protein Interactions using GFP Affinity Purification and SILAC-based Quantitative Mass Spectrometry -- Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC -- Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling -- Protein Correlation Profiling-SILAC to Study Protein-Protein Interactions -- Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC -- Design and Application of Super-SILAC for Proteome Quantification -- Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies -- Analysis of Secreted Proteins using SILAC -- Identification of MicroRNA Targets by Pulsed SILAC -- MaxQuant for In-Depth Analysis of Large SILAC Datasets
    Abstract: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research
    Pages: XIV, 372 p. 65 illus., 27 illus. in color. : online resource.
    ISBN: 9781493911424
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  • 31
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: The Hematopoietic Stem Cell Landscape -- Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow -- Single-Cell PCR Profiling of Gene Expression in Hematopoiesis -- Hematopoietic Stem and Progenitor Cell Mobilization in Mice -- Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells -- Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells -- Gene Expression Profiling of Hematopoietic Stem Cells (HSC) -- Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells -- DNA Methylation Profiling of Hematopoietic Stem Cells -- Metabolic Characterization of Hematopoietic Stem Cells -- Nano Proteomic Assays on Hematopoietic Stem Cells -- Hematopoietic Differentiation of Pluripotent Stem Cells in Culture -- Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells -- Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells
    Abstract: Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases.℗ An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation
    Pages: XII, 364 p. 52 illus., 42 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493911332
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  • 32
    Keywords: Medicine ; Human Physiology ; Neurosciences ; Biomedicine ; Neurosciences ; Human Physiology ; Springer eBooks
    Description / Table of Contents: History of Electrophysiology and the Patch-Clamp.-℗ Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications -- Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers -- State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening -- Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch℗ʼ 96 Using Instant Frozen Cells -- Planar Patch Clamp for Neuronal Networkś€“Considerations and Future Perspectives -- Methods for the Study of Synaptic Receptor Functional Properties -- Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity -- Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique -- Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability -- Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices -- Outside-Out ́€˜Sniffer-patch́€™ Clamp Technique for in situ Measures of Neurotransmitter Release -- A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices -- Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics -- Synaptic Connectivity in Engineered Neuronal Networks -- Modeling of Action Potential Generation in NG108-15 Cells -- Whole-Cell Patch-Clamp Recordings in Freely Moving Animals -- Pressure-Polished Borosilicate Pipettes are €œÚniversal Sealeŕ€ Yielding Low Access Resistance and Efficient Intracellular Perfusion -- Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes -- Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents -- Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI -- Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes -- Principles of Single-Channel Kinetic Analysis
    Abstract: Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies, and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology, and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals, and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Patch-Clamp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators
    Pages: XI, 403 p. 105 illus., 41 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493910960
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  • 33
    Keywords: Life sciences ; Human Physiology ; Endocrinology ; Microbiology ; Life sciences ; Microbiology ; Endocrinology ; Human Physiology ; Springer eBooks
    Description / Table of Contents: Part I: Basic Concepts Underlying the Microbiota-Gut-Brain Axis -- Microbial Endocrinology and the Microbiota-Gut-Brain Axis -- Utilizing €œómicś€ Tools to Study the Complex Gut Ecosystem -- The Enteric Nervous System and Gastrointestinal Innervation: Integrated Local and Central Control -- Intestinal Barrier Function and the Brain-Gut Axis -- Vagal Pathways for Microbiome-Brain-Gut Axis Communication -- The Brain-Gut Axis in Health and Disease -- Part II: Mechanistic Factors Influencing the Microbiota-Gut-Brain Axis -- Gastrointestinal Hormones and Their Targets -- Microbiome, HPA Axis and Production of Endocrine Hormones in the Gut -- Neuropeptides and the MicrobiotထGut́€‘Brain Axis -- Bacterial Neuroactive Compounds Produced by Psychobiotics -- Multidirectional Chemical Signalling Between Mammalian Hosts, Resident Microbiota, and Invasive Pathogens:℗ Neuroendocrine Hormone-Induced Changes in Bacterial Gene Expression -- Influence of Stressor-Induced Nervous System Activation on the Intestinal Microbiota and the Importance for Immunomodulation -- Part III: The Microbiota-Gut-Brain Axis in Health and Disease -- The Effects of Inflammation, Infection and Antibiotics on the Microbiota-Gut-Brain Axis -- Microbiota, Inflammation and Obesity -- Microbiota, Immunoregulatory Old Friends and Psychiatric Disorders -- Microbiota-Gut-Brain Axis and Cognitive Function -- The Impact of Microbiota on Brain and Behavior: Mechanisms & Therapeutic Potential -- Neuroimaging the Microbiome-Gut́€“Brain Axis -- The Future of Probiotics for Disorders of the Brain-Gut Axis -- Index
    Abstract: The field of microbial endocrinology is expressly devoted to understanding the mechanisms by which the microbiota (bacteria within the microbiome) interact with the host (€œúś€).℗ This interaction is a two-way street and the driving force that governs these interactions are the neuroendocrine products of both the host and the microbiota. Chapters include neuroendocrine hormone-induced changes in gene expression and microbial endocrinology and probiotics. This is the first in a series of books℗ dedicated to understanding how bi-directional communication between host and bacteria represents the cutting edge of translational medical research, and hopefully identifies new ways to understand the mechanisms that determine health and disease
    Pages: XVIII, 436 p. 48 illus., 19 illus. in color. : online resource.
    ISBN: 9781493908974
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  • 34
    Keywords: Life sciences ; Ecology ; Conservation biology ; Forests and forestry ; Environmental sciences ; Nature conservation ; Life sciences ; Ecology ; Forestry Management ; Conservation Biology/Ecology ; Environment, general ; Nature conservation ; Springer eBooks
    Description / Table of Contents: Part I.℗ ℗ Introduction -- Deputy Chief of FS Research, Raphael Zon, Memo to℗ Forest Service Chief Pinchot, May 6, 1908 -- Map of USDA Forest Service Experimental Forest and Ranges -- Chapter 1. A Grand Experiment: USDA Forest Service Experiment Forest and Ranges -- Chapter 2. Forest Service Experimental Forests and Long-term Data Sets: Stories of their Meaning to Station Directors -- Part II.℗ ℗ Research Trajectories in Forest Ecology, Management, and Silviculture for Conifers -- Chapter 3. The Value of Old Forests: Lessons from the Forest Service Reynolds Research Natural Areas -- Chapter 4. Restoring a legacy: Long-leaf pine research at the Forest Service Escambia Experimental Forest -- Chapter 5. Northern Conifer Research: Multiple Species and Multiple Values -- Chapter 6. Fire and Thinning Research on Forest Service Blacks Mountain Experimental Forest -- Part III.℗ ℗ Research Trajectories in Forest Ecology, Management, and Silviculture for Hardwoods -- Chapter 7. Uneven-aged management after a half-century of research on the Forest Service Fernow Experimental Forest in West Virginia -- Chapter 8. An Alternative to Clearcutting: A Partnership between Scientists on the Fernow Experimental Forest and Land Managers on the Monongahela National Forest -- Chapter 9. Development of the Selection System in Northern Hardwood Forests: An 80 Year Research Legacy with Impacts across Borders -- Part IV.℗ ℗ Research Trajectories in Wildlife, Fauna, Insects -- Chapter 10, The Starkey Experimental Forest and Range: Long-Term Research to meet the needs of Managers -- Chapter 11. Evolution of a Short-Term Study of Lodgepole Pine Dwarf Mistletoe Vectors that turned Into a Long-Term Study of the Remarkable Gray Jay on the Fraser Experimental Forest, Co, 1982-2009 -- Chapter 12. Subterranean Termite Control Examinations on Current And Former Experimental Forests and Ranges -- Part V.℗ ℗ Research Trajectories in Forest and Range Hydrology, Biogeochemistry, Soils and Ecosystem Science -- Chapter 13. A Cycle of Research at Fort Valley, Arizona, the First Forest Service Research Experiment Station -- Chapter 14. Contributions of Studies on Experimental Forests to Hydrology and Watershed Management -- Chapter 15. A History of Watershed Research in Experimental Forests of the Interior Highlands -- Chapter 16. Research Related to Roads in USDA FS Experimental Forests -- Chapter 17. The Role of Experimental Forests and Ranges in the Development of Ecosystem Science and Biogeochemical Cycling Research -- Chapter 18. Six Decades of Soil and Ecosystem Research at USDA FS Calhoun Experimental Forest -- Chapter 19. Disturbance Ecology and Tropical Forest Management at the Luquillo Experimental Forest -- Chapter 20. The Acid Deposition Trajectory -- Part VI.℗ ℗ Research Trajectories in Fire -- Chapter 21. From Fighting Bad Fires to Fire-Dependent Ecosystems -- Chapter 22. The Fire Research Program at the Silas Little Experimental Forest, New Lisbon, New Jersey -- Part VII.℗ ℗ Intersite and Network Trajectories -- Chapter 23. The Key Roles of Four Experimental Forests in the Long Term Soil Productivity International Research Program -- Chapter 24. The Role of the Hydro Database in Developing the Water Hydrology and Chemistry Network -- Part VIII. Emerging Human Dimension Research Trajectories -- Chapter 25. Social Science Research and Experimental Forests and Ranges -- Chapter 26. Public Use and Citizen Outreach in Experimental Forests and Ranges -- Chapter 27. Expanding the Vision of Experimental Forests to Urban Areas -- Part IX.℗ ℗ The Future -- Chapter 28. The Future of USDA Forest Service Experimental Forests and Ranges
    Abstract: Long-term Forest Service research studies are the source of ideas, tools and research methods that we now take for granted.℗ These range from the effects of fire exclusion on forest and rangeland structure and composition through modern tree care and vegetation control. Innovative experimental designs, such as the use of paired watersheds to understand the effects of forest management on water quality and quantity, were developed on USDA Forest Service Experimental Forests and Ranges (EFRs). The stories of these and other innovations are told in this volume. ℗ EFRs are scientific treasures, providing secure, protected research sites where complex and diverse ecological processes are studied over the long term.℗ This book offers several examples of the dynamic interactions among questions of public concern or policy, EFR research, and natural resource management practices and policies.℗ Often, trends observed ́€“ or expected -- in the early years of a research program are contradicted or confounded as the research record extends over decades.℗ The EFRs are among the few areas in the US where such long-term research has been carried out by teams of scientists.℗ Changes in societý€™s needs and values can also redirect research programs. Each chapter of this book reflects the interplay between the ecological results that emerge from a long-term research project and the social forces that influence questions asked and resources invested in ecological research. While these stories include summaries and syntheses of traditional research results, they offer a distinctly new perspective, a larger and more complete picture than that provided by a more typical 5-year study.℗ They also provide examples of long-term research on EFRs that have provided answers for questions not even imagined at the time the study was installed
    Pages: XXII, 672 p. 187 illus., 105 illus. in color. : online resource.
    ISBN: 9781461418184
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  • 35
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    New York, NY : Springer
    Keywords: Life sciences ; Biological models ; Developmental Biology ; Plant Ecology ; Botany ; Plant anatomy ; Plant physiology ; Life sciences ; Plant Sciences ; Plant Ecology ; Systems Biology ; Developmental Biology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Introduction -- Definition of Celiac Disease and Gluten Sensitivity -- Epidemiology of Celiac Disease -- The Pathophysiology of Celiac Disease -- Immunogenetics of Celiac Disease -- Using Animal Models of Celiac Disease to℗ Understand the Role of MHC II -- Role of Gut Microbes in Celiac Disease Risk and Pathogenesis -- Presentation of Celiac Disease in Children and Adults -- Diagnosis of Celiac Disease -- Nutrition in Celiac Disease -- Dietary Supplements in Celiac Disease -- Monitoring and Follow-up of Patients with Celiac Disease -- Special Considerations in Children and Young Adults with Celiac Disease -- Novel and Experimental Therapies on the Horizon -- Morbidity and Mortality Associated with Celiac Disease -- Refractory Celiac Disease -- Celiac Disease: Dispelling Misconceptions and Myths -- Summary of Recommendations from the American College of Gastroenterology Celiac Disease Guidelines
    Abstract: This volume addresses recent developments in weed science. These developments include conservation agriculture and conservation tillage, climate change, environmental concerns about the runoff of agrochemicals, resistance of weeds and crops to herbicides, and the need for a vastly improved understanding of weed ecology and herbicide use. The book provides details on harnessing knowledge of weed ecology to improve weed management in different crops and presents information on opportunities in weed management in different crops. Current management practices are also covered, along with guidance for selecting herbicides and using them effectively. Written by experts in the field and supplemented with instructive illustrations and tables, Recent Advances in Weed Management is an essential reference for agricultural specialists and researchers, government agents, extension specialists, and professionals throughout the agrochemical industry, as well as a foundation for advanced students taking courses in weed science
    Pages: XI, 411 p. 36 illus., 23 illus. in color. : online resource.
    ISBN: 9781493910199
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  • 36
    Keywords: Medicine ; Oncology ; Human Genetics ; Proteomics ; Biomedicine ; Cancer Research ; Human Genetics ; Proteomics ; Springer eBooks
    Description / Table of Contents: Genome-Wide Mapping Of RNA Pol-II Promoter Usage In Mouse Tissues By Chip-Seq -- Using Native Chromatin Immunoprecipitation To Interrogate Histone Variant Protein Deposition In Embryonic Stem Cells -- Reduced Representation Bisulfite Sequencing To Identify Global Alteration In The DNA Methylation -- A High-Throughput Microrna Expression Profiling System -- Using Pooled Mir30-Shrna Library For Cancer Lethal And Synthetic Lethal Screens -- Genome-Wide RNA Interference Screening To Identify Regulators Of Epigenetic Silencing Of A Tumor Suppressor Gene -- A Diphtheria Toxin Negative Selection In RNA Interference Screening -- Cancer Metabolism: Crosstalk Between Signaling And O-Glcnacylation -- Targeted Genome Modification Via Triple Helix Formation -- Synthesis Of Stabilized Alpha-Helical Peptides -- Arginine-Grafted Biodegradable Polymer: A Versatile Transfection Reagent For Both DNA And Sirna -- Using Laco Arrays To Monitor DNA Double-Strand Break Dynamics In Live Schizosaccharomyces Pombe Cells -- Zebrafish As A Platform To Study Tumor Progression -- Clonal Screens To Find Modifiers Of Partially Penetrant Phenotypes In C. Elegans -- Serum Profiling Using Protein Microarrays To Identify Disease Related Antigens -- Interrogation Of In Vivo Protein-Protein Interactions Using Transgenic Mouse Models And Stable Isotope Labeling -- New Biophysical Methods To Study The Membrane Activity Of Bcl-2 Proteins -- Purification Of Recombinant 2XMBP Tagged Human Proteins From Human Cells -- Computational Analysis In Cancer Exome Sequencing -- Matrix Factorization Methods For Integrative Cancer Genomics -- Computational Methods For DNA Copy-Number Analysis Of Tumors
    Abstract: Cancer Genomics and Proteomics: Methods and Protocols, Second Edition ℗ includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Cancer Genomics and Proteomics: Methods and Protocols, Second Edition seeks to aid scientists in the further study into various aspects of tumor initiation and progression
    Pages: XI, 262 p. 44 illus., 23 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781493909926
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  • 37
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Plant anatomy ; Microscopy ; Life sciences ; Plant Anatomy/Development ; Cell Biology ; Developmental Biology ; Biological Microscopy ; Springer eBooks
    Description / Table of Contents: FM Preface -- 1 Nuclei and Chromosomes -- 2 Mitochondria -- 3 Chloroplasts -- 4 The Endoplasmic Reticulum, Golgi Apparatuses, and Endocytic Organelles -- 5 Vacuoles and Storage Organelles -- 6 Cytoskeletons -- 7 Cell Walls -- 8 Generative Cells -- 9 Meristems
    Abstract: This atlas presents beautiful photographs and 3D-reconstruction images of cellular structures in plants, algae, fungi, and related organisms taken by a variety of microscopes and visualization techniques. Much of the knowledge described here has been gathered only in the past quarter of a century and represents the frontier of research. The book is divided into nine chapters: Nuclei and Chromosomes; Mitochondria; Chloroplasts; Endoplasmic Reticulum, Golgi Apparatuses, and Endocytic Organelles; Vacuoles and Storage Organelles; Cytoskeletons; Cell Walls; Generative Cells; and Meristems. Each chapter includes several illustrative photographs accompanied by a short text explaining the background and meaning of the image and the method by which it was obtained, with references. Readers can enjoy the visual tour within cells and will obtain new insights into plant cell structure. This atlas is recommended for plant scientists, students, their teachers, and anyone else who is curious about the extraordinary variety of living things
    Pages: XXV, 202 p. 92 illus., 66 illus. in color. : online resource.
    ISBN: 9784431549413
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  • 38
    Keywords: Medicine ; Neurosciences ; Psychiatry ; Psychopharmacology ; Biomedicine ; Neurosciences ; Psychopharmacology ; Psychiatry ; Springer eBooks
    Description / Table of Contents: Neurogenetics of Aggressive Behavior Studies in Rodents -- Neurogenetics of Aggressive Behavior Studies in Primates -- Glucocorticoids and Escalated Aggressive Behavior. Models of Female Aggression Prefrontal Cortex. Nitric Oxide, Serotonin and Aggression -- Hypothalamus and Aggressive Behavior -- Vasopressin-Serotonin Interactions in Aggressive Behavior -- Dopamine-oxytocin: Attachment and Conflict -- Stress and violence. Gene-environment interactions and violence. MAO-A and genetic risk for violence -- The development of violent behavior in humans -- Schizophrenia and violence. Psychopathy -- Violent and callous behavior in children -- Aggression in Children and Adolescents- Alcohol and Aggression
    Abstract: This volume assembles the leading aggression researchers both at the preclinical and clinical level. They review the current state of knowledge about neural mechanisms of aggressive behavior and point to the need for innovative methodologies to further our understanding of this greatly understudied set of behaviors
    Pages: VIII, 479 p. 50 illus., 12 illus. in color. : online resource.
    ISBN: 9783662442814
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  • 39
    Keywords: Life sciences ; DNA microarrays ; Proteomics ; Bioinformatics ; Biology / Data processing ; Life sciences ; Bioinformatics ; Proteomics ; Microarrays ; Computer Appl. in Life Sciences ; Springer eBooks
    Description / Table of Contents: Introduction -- The Unknown Proteins Coded by the Human Chromosome -- Chromosome Transcript Profiling -- Standardization of Proteome Annotations -- Mass Spectrometry Protein Sequencing Technology Platforms -- Post Translational Modifications within the Human Proteome -- NAPPA Array - Protein Microarray Technology -- Clinical Bioinformatics - A New Emerging Science -- Computational Science within Genomics and Proteomics -- Rapid Advances in the Field of Epigenetics
    Abstract: This book offers a valuable resource that allows students, researchers, educators and the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map was presented in 2002, and the Proteome Sequence Map is currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas and shape major areas of the pharmaceutical and biomedical industries. The book provides background information on and our current understanding of these gene and protein areas, and explains in detail how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient treatment and care. The book will benefit all students and researchers who need a good understanding of genomics and proteomics within the clinical field. Its content is also suitable for a broad readership, including those not specialized in this field. Dr. Marko-Varga is head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University. He's also Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan
    Pages: VIII, 282 p. 80 illus., 65 illus. in color. : online resource.
    ISBN: 9789401792028
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  • 40
    Keywords: Life sciences ; Neurosciences ; Neurochemistry ; Biochemistry ; Animal genetics ; Neurobiology ; Life sciences ; Protein Science ; Neurosciences ; Neurochemistry ; Animal Genetics and Genomics ; Biochemistry, general ; Neurobiology ; Springer eBooks
    Description / Table of Contents: 1. Introduction -- 2. Historical and Topological Perspective of Connexins -- 3. Molecular Components and Nomenclature of Gap Junctions -- 4. General Cell Biology of Connexins -- 5. Genomic Organization of Connexins -- 6. Transcriptional Regulation of the Connexin Gene -- 7. Epigenetic Regulation of Connexins -- 8. Translation Regulation of Connexins -- 9. Interaction Partners of Connexin Proteins -- 10. Tissue Distribution of Connexins -- 11. Connexin Functions -- 12. Connexin Mutations and Disease -- 13. Suggested Reading
    Abstract: Gap-junction proteins, connexins are important cellular entities that regulate various facets of cellular physiology. The connexins constitute large family of proteins.℗ The present book provides basic overview about various aspects of connexin proteins. The book has tried to touch the fundamental aspects of connexin family. This book will be useful to broad audience that includes under-and post-graduate students, research scholars, clinicians, etc. The reference section at the end of the book will be helpful for those who seek to know deeper details about this family of proteins
    Pages: XV, 93 p. 4 illus. in color. : online resource.
    ISBN: 9788132219194
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  • 41
    Keywords: Life sciences ; Geography ; Agriculture ; Sustainable development ; Life sciences ; Agriculture ; Climate Change/Climate Change Impacts ; Sustainable development ; Economic Geography ; Trade ; Springer eBooks
    Description / Table of Contents: 1. Population, agriculture, poverty and food security: an overview -- 2. Policies and legislations governing marketing and food trade: Southern Africa -- 3. Urban, peri-urban agriculture and food security among small-scale agricultural producers: Southern Africa -- 4. Indigenous plant resources and food security among small-scale agricultural producers: Southern Africa -- 5. Role of small stock in safeguarding food security in dry lands: case in Namibia -- 6. Improving productivity, food safety and nutritional levels of dependable food crops: Southern Africa -- 7. Climate-smart agriculture: incorporating weather information into small-scale agricultural producers' system: South Africa
    Abstract: The book focuses on food security highlighting the role of indigenous knowledge and scientific research in addressing the plight of poor small-scale agricultural producers. Rapidly growing global population and global policies and management governing sustainability, hunger, food security and poverty alleviation are discussed. Additionally, impacts of probable climate change, research on land productivity and performance of dependable food crops i.e. cassava and pearl millet are discussed. Analyzed in great detail are roles of small stock, urban/peri-urban agriculture and advantages of climate-smart agriculture and participatory research in enhancing food security of the small-scale agricultural producers in Southern Africa
    Pages: XVI, 173 p. 35 illus., 26 illus. in color. : online resource.
    ISBN: 9783319094953
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  • 42
    Keywords: Medicine ; Oncology ; Toxicology ; Food science ; Chemical engineering ; Endocrinology ; Environmental Medicine ; Biomedicine ; Pharmacology/Toxicology ; Environmental Health ; Cancer Research ; Endocrinology ; Food science ; Industrial Chemistry/Chemical Engineering ; Springer eBooks
    Description / Table of Contents: Human Health Effects of Bisphenol A -- Phthalates in Food Packaging, Consumer Products, and Indoor Environments -- Brominated Flame Retardants and Their Replacements in Food Packaging and Household Products' Uses, Human Exposure, and Health Effects -- Nanoparticles in Polymer Nanocomposite Food Contact Materials' Uses, Potential Release, and Emerging Toxicological Concerns -- The Alkylphenols Nonylphenol and Octylphenol in Food Contact Materials and Household Products' Exposure and Health Risk Considerations -- Benzophenone UV-Photoinitiators Used in Food Packaging' Potential for Human Exposure and Health Risk Considerations -- Perfluorinated Compounds in Food Contact Materials -- Antimony in Food Contact Materials and Household Plastics Uses, Exposure and Health Risk Considerations -- Lead in Household Products -- Methylnaphthalene in Food Packaging and Cadmium in Food Packaging and Household Items Overview of Exposure, Toxicology, Regulatory Aspects, and Research Needs -- Food Contact Materials: Practices, Agencies and Challenges
    Abstract: This book serves as a comprehensive resource on toxicants that can be released from food packaging materials and household plastics. Chapters include sources and levels of chemical exposure, known and suspected health effects, and the identification of data gaps with recommendations for further research. In addition, regulatory approaches and risk assessment challenges in the United States and Europe are discussed. ℗ Chapters cover both the more widely known chemicals that can migrate from food packaging (bisphenol A, perfluorinated chemicals), and household plastics (lead, phthalates, brominated flame retardants), as well as chemicals that are just entering use in food packaging (nanomaterials in polymer food packaging), and chemicals recently identified as migrating from food packaging to food stuffs (phthalates, benzophenones, antimony, methylnaphthalene, and the alkylphenols nonylphenol and octylphenol). Chapters on phthalates and brominated flame retardants discuss challenges that arise with the use of replacement chemicals. The health effect sections of chapters have drawn on a wide variety of toxicological endpoints, and recommend approaches to better assess toxicological risks in vulnerable human populations. ℗ Reflecting the global nature of our food supply and household consumer goods, contributions have been drawn from international experts. A wide range of scientists will find this book to be useful, including toxicologists, environmental health scientists, food scientists, and regulators
    Pages: X, 310 p. 20 illus., 7 illus. in color. : online resource.
    ISBN: 9781447165002
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  • 43
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    New York, NY : Springer
    Keywords: Life sciences ; Proteomics ; Biochemistry ; Life sciences ; Protein Science ; Proteomics ; Springer eBooks
    Description / Table of Contents: History of Plant Lectin Research -- Fungal Lectins a Growing Family -- The “White Kidney Bean Incident” in Japan -- Hemagglutination (Inhibition) Assay -- Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources -- High-Performance Lectin Affinity Chromatography -- Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography -- Lectin-Probed Western Blot Analysis -- Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins -- Sialidase-dependent Binding Assay for Influenza and Related Viruses -- Lectin Affinity Electrophoresis -- Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans -- Basic Procedures for Lectin Flow Cytometry -- Histochemical Staining Using Lectin Probes -- Equilibrium Dialysis using Chromophoric Sugar Derivatives -- Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars -- Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions -- Isothermal Calorimetric Analysis of Lectin–sugar Interaction -- Carbohydrate–Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines -- Lectin-Based Glycomics: How and When Was the Technology Born? -- Frontal Affinity Chromatography (FAC): Theory and Basic Aspects -- Advanced FAC with a Library of Fluorescent-Labeled Oligosaccharides -- Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization -- Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces -- Application of Lectin Microarray to Bacteria Including Lactobacillus casei Shirota -- Live C
    Abstract: Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on €œéngineeŕ€ novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists
    Pages: XIII, 613 p. 168 illus., 60 illus. in color. : online resource.
    ISBN: 9781493912926
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  • 44
    Keywords: Life sciences ; Biochemistry ; Cytology ; Microbiology ; Plant breeding ; Life sciences ; Cell Biology ; Microbiology ; Plant Genetics & Genomics ; Plant Biochemistry ; Springer eBooks
    Description / Table of Contents: Valuable Secondary Metabolites from Fungi -- Penicillins -- Cephalosporins -- Cyclosporines: Biosynthesis and Beyond -- Aflatoxin Biosynthesis: Regulation and Subcellular Localization -- Roquefortine C and Related Prenylated Indole Alkaloids -- Ochratoxin A and Related Mycotoxins -- Carotenoids -- Astaxanthin and Related Xanthophylls -- Gibberellins and the Red Pigments Bikaverin and Fusarubin -- Fusarins and Fusaric Acid in Fusaria -- Lovastatin, Compactin, and Related Anticholesterolemic Agents -- Meroterpenoids -- Ergot Alkaloids -- Fungal NRPS-dependent Siderophores: from Function to Prediction
    Abstract: This volume describes the more relevant secondary metabolites of different fungi with current information on their biosynthesis and molecular genetics. Bolstered with color illustrations and photographs, the book describes the possible application of molecular genetics to directed strain improvement in great detail. The needs for future developments in this field are also discussed at length. ℗ Written by authorities in the field, Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites provides a cutting-edge perspective on fungal secondary metabolism and underlying genetics and is a valuable resource for scientists, researchers, and educators in the field of fungal biology
    Pages: XI, 351 p. 65 illus., 35 illus. in color. : online resource.
    ISBN: 9781493911912
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  • 45
    Keywords: Medicine ; Neurosciences ; Cell receptors ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Receptors ; Springer eBooks
    Description / Table of Contents: 1. Introduction: G-protein Signaling in the Retina -- Part One- Phototransduction in Rods and Cones -- 2. G-protein Deactivation Mechanisms in Vertebrate Phototransduction -- 3. Signaling by Rod and Cone Photoreceptors: Opsin Properties, G-protein Assembly and Mechanisms of Activation -- 4. G-protein€“éffector Coupling in the Vertebrate Phototransduction Cascade -- Part II- Inner Retinal GPCR Signaling Pathways -- 5. Interdependence among Members of the mGluR6 G-protein Mediated Signalplex of Retinal Depolarizing Bipolar Cells -- 6. Mechanistic Basis for G-protein Function in ON Bipolar Cells -- 7. Modulation of Trpm1 and the mGluR6 cascade in ON Bipolar Cells -- 8. The Role of Dopamine in Fine-tuning Cone- and Rod-driven Vision -- 9. Regulation of Electrical Synaptic Plasticity in the Retina by G-protein Coupled Receptors -- Part III- Signaling by Photosensitive Ganglion Cells -- 10. The Functional Properties of the G-protein-coupled Receptor melanopsin in Intrinsically Photosensitive Retinal Ganglion Cells
    Abstract: We have learned a great deal about the organization and function of GPCR pathways, and the role that they play in vision. The information gained from these studies has provided, and will continue to provide, critical insights to further our understanding of complex GPCR pathways in the central nervous and endocrine systems. In essence, the retina is the first €˜óptogeneticallý€™-driven circuit with clearly understood physiology. Thus, studying GPCR-driven pathways in the retina will likely guide the interpretation of optogenetic experiments, which are increasingly utilized to study central circuits. Collectively, these signaling pathways allow the retina to represent visual space over a wide range of light intensities and to synchronize its function to the day/night cycle. G protein Signaling Mechanisms in the Retina summarizes our current understanding of the organizational principles of GPCR pathways, using insights derived from the study of the retina. The book highlights several G protein signaling cascades, including phototransduction, ON bipolar cell signaling, dopaminergic pathways, and ipRGC signaling
    Pages: VIII, 198 p. 35 illus., 26 illus. in color. : online resource.
    ISBN: 9781493912186
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  • 46
    Keywords: Life sciences ; Microbiology ; Botany ; Plant anatomy ; Plant Diseases ; Plant physiology ; Plant breeding ; Life sciences ; Plant Sciences ; Plant Pathology ; Microbiology ; Plant Anatomy/Development ; Plant physiology ; Plant Breeding/Biotechnology ; Springer eBooks
    Description / Table of Contents: Fungal Diseases of Groundnut: Control and Future Challenges -- Plant Growth Promoting Rhizobacteria in Crop Protection and Challenges -- Understanding the Mechanism Involved in PGPR Mediated Growth Promotion and Suppression of Biotic and Abiotic Stress in Plants -- Downy Mildew Disease of Pearl Millet and Its Control -- Research on Plant Pathogenic Fungi in the Genomics Era: From Sequence Analysis to Systems Biology -- Pre and Post Harvest Diseases of Potato and Their Management -- Host-Pathogen Interaction, Plant Diseases, Disease Management Strategies and Future Challenges -- Ug99-Future Challenges -- Increased Virulence of Wheat Rusts and the Threat to Global Crop Production -- Fusarium Diseases of Canadian Grain Crops: Impact and Disease Management Strategies -- Pseudomonas fluorescens: A Potential Bio-control Agent for Management of Fungal Diseases of Crop Plants -- Oat Fungal Diseases and the Application of Molecular Marker Technology for Their Control
    Abstract: This volume presents the issues and challenges of crop pathogens and plant protection. Composed of the latest knowledge in plant pathology, the book covers topics such as fungal diseases of the groundnut, plant growth promoting rhizobacteria, plant pathogenic fungi in the genomics era, the increased virulence of wheat rusts, and oat fungal diseases. Written by experienced and internationally recognized scientists in the field, Future Challenges in Crop Protection Against Fungal Pathogens is a concise yet comprehensive resource valuable for both novice as well as experienced plant scientists and researchers
    Pages: XIII, 364 p. 68 illus., 47 illus. in color. : online resource.
    ISBN: 9781493911882
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  • 47
    Keywords: Medicine ; Neurosciences ; Neurochemistry ; Neurology ; Psychiatry ; Biomedicine ; Neurosciences ; Neurochemistry ; Neurology ; Psychiatry ; Springer eBooks
    Description / Table of Contents: General Pathophysiology of Neuroglia: Neurological and Psychiatric Disorders as Gliopathies -- Ionic Signaling in Physiology and Pathophysiology of Astroglia -- Pathophysiology of Vesicle Dynamics in Astrocytes -- Glial Glutamate and Metabolic Transporters as a Target for Neurodegenerative Therapy and Biomarkers -- Alexander Disease and Astrotherapeutics -- Role of Astrocytes in Central Nervous System Traum -- Astroglial and Neuronal Integrity During Cortical Spreading Depolarization -- Crucial role for astrocytes in epilepsy -- Microglial Biology in Neuoinflammatory Disease: Phamaco-industrial Approach to Target Validation -- The Role of Astrocytes in Huntington's Disease -- Amyotrophic Lateral Sclerosis: A Glial Perspective -- Complex and Associated Astroglial and Microglial Changes during the Progression of Alzheimer's Disease -- Possible Therapeutic Targets in Microglia -- Novel Therapeutic Approaches to Malignant Gliomas -- Hepatic encephalopathy: A Primary Neurogliopathy -- Astroglia and Severe Mental Illness: A Role for Glutamate Microdomains -- Emerging Roles for Glial Pathology in Addiction -- Astroglial Connexins as Elements of Sleep-wake Cycle Regulation and Dysfunction -- Experimental Treatment of Acquired and Inherited Neuropathies -- Satellite glial cells as target for chronic pain therapy -- Enteric Glial Cells: Implications in Gut Pathology
    Abstract: Pathophysiological states, neurological and psychiatric diseases are almost universally considered from the neurocentric point of view, with neurons being the principal cellular element of pathological process. The brain homeostasis, which lies at the fulcrum of healthy brain function, the compromise of which invariably results in dysfunction/disease, however, is entirely controlled by neuroglia. It is becoming clear that neuroglial cells are involved in various aspects of initiation, progression and resolution of neuropathology. In this book we aim to integrate the body of information that has accumulated in recent years revealing the active role of glia in such pathophysiological processes. Understanding roles of glial cells in pathology will provide new targets for medical intervention and aide the development of much needed therapeutics. This book will be particularly useful for researchers, students, physicians and psychotherapists working in the field of neurobiology, neurology and psychiatry
    Pages: XVI, 541 p. 74 illus., 48 illus. in color. : online resource.
    ISBN: 9781493909742
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  • 48
    Keywords: Medicine ; Oncology ; Biomedicine ; Cancer Research ; Molecular Medicine ; Biomedicine general ; Springer eBooks
    Description / Table of Contents: Overview of Vitamin C and Cancer -- Anticancer Mechanisms of Vitamin C -- Potential Therapeutics for Vitamin C and Cancer -- Conclusion -- Appendix
    Abstract: Research on vitamin C and its effects on cancer is growing in popularity around the world as positive research continues to accumulate building a stronger case for its effectiveness. This concise SpringerBrief on Vitamin C and Cancer presents the latest findings on how vitamin C induces apoptosis. A high concentration of vitamin C allows for ascorbate to generate hydrogen peroxide in tissue that can selectively kill cancer cells Research has confirmed that high-dose vitamin C is cytotoxic to a wide variety of cancer cell lines, and that it also boosts the anti-cancer activity of several common chemotherapy drugs. Vitamin C also does more than just kill cancer cells. It boosts immunity by stimulating collagen formation to help the body wall off the tumor. It inhibits hyaluronidase, an enzyme that tumors use to metastasize and invade other organs throughout the body. This concise and up-to-date Brief is geared towards cancer researchers and scientists, as well as physicians interested in the basic science and the translational potential of vitamin C in cancer therapeutics
    Pages: VIII, 69 p. 1 illus. : online resource.
    ISBN: 9781493918904
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  • 49
    Keywords: Medicine ; Toxicology ; Forensic Medicine ; Forensic Medicine ; Pathology ; Pediatrics ; Biomedicine ; Forensic Science ; Forensic Medicine ; Pharmacology/Toxicology ; Pathology ; Pediatrics ; Springer eBooks
    Description / Table of Contents: A History of Pediatric Forensic Pathology -- Pediatric Death Scene Investigation -- The Pediatric Autopsy -- Fetal, Intrapartum, and Neonatal Deaths -- Placental and Maternal Conditions in Perinatal Deaths -- Birth Trauma -- Neonaticide -- Sudden Infant Death Syndrome (SIDS), Sudden Unexpected Death in Infancy (SUDI), and Sudden Unexplained Death in Childhood (SUDC) -- Pediatric Asphyxial Deaths -- Factitious Illness by Proxy in Children -- Cutaneous Findings in Children -- Skin Conditions Mimicking Pediatric Inflicted Injury -- Blunt Abdominal and Thoracic Injuries in Children -- Cardiopulmonary Resuscitation Injuries in Children -- Evaluation of Pediatric Fractures at Autopsy -- Dating of Other Injuries in Children -- Fatal Pediatric Craniocerebral and Spinal Trauma -- Biomechanics of Pediatric Head Injury -- Ocular Findings in Pediatric Inflicted Injury -- Neuroimaging of Pediatric Inflicted Injury -- Non-Central Nervous System Imaging of Pediatric Inflicted Injury -- Adolescent Homicide -- Pediatric Sexual Abuse -- Starvation, Malnutrition, Dehydration, and Fatal Neglect -- Pediatric Suicide -- Sports-Related Injuries and Deaths -- Other Pediatric Accidental Deaths -- Pediatric Iatrogenic Deaths -- Pediatric Toxicology -- Pediatric Natural Deaths -- Cardiac Channelopathies and the Molecular Autopsy -- Other Pediatric Cardiac Conditions -- Childhood Obesity -- Pediatric Metabolic Diseases -- The Disabled Child -- Pediatric Anthropology and Odontology -- Ancillary Studies and Dissection Techniques in the Pediatric Autopsy -- Pediatric Organ and Tissue Donation -- Death Certification -- Expert Testimony in Pediatric Forensics -- Appendices ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗
    Abstract: The investigation of sudden or unexplained death of children represents a unique medical and forensic area of study. Children have unique anatomic and functional characteristics that must be recognized and understood by the medical investigator. Further complicating this process is the fact that the anatomic structure, composition and function of various organs and organ systems in the pediatric population change throughout the developmental stages of childhood. The disease processes, reactions to trauma and risk factors for accidental death and homicide change from infancy through adolescence. Forensic Pathology of Infancy and Childhood provides an authoritative, comprehensive reference text devoted to the medicolegal investigation of sudden unexpected death in children. With contributions from internationally renowned experts, individual chapters focus on specific unique causes and organ systems with detailed accounts of the changes in fatal diseases, risk factors of causes of sudden death, and responses to fatal trauma that occur as a child grows from neonatal stage through infancy, toddlerhood, childhood and adolescence. This text is an essential reference resource for forensic pathologists, medical examiner offices, pediatric pathologists, pediatric hospitals, anatomic pathologists and those in training as well as those in related legal professions
    Pages: XVIII, 1303 p. 686 illus., 524 illus. in color. eReference. : online resource.
    ISBN: 9781617794032
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