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  • Springer  (119,131)
  • 2015-2019  (3,406)
  • 2005-2009  (523)
  • 1970-1974  (115,206)
  • 1
    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 2
    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 3
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 4
    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 5
    New York, NY : Springer
    Keywords: Immunology ; Medical virology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus -- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV -- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR) -- Establishment of Replication-competent HCV Strain with Minimum Modifications -- Full-length Open Reading Frame Amplification of Hepatitis C Virus -- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing -- A Cell Culture Model for Persistent HCV Infection -- Expression Cloning of Host Factors Required for the HCV Replication Cycle -- Application of Deep Mutational Scanning in Hepatitis C Virus -- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals -- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay -- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells -- Functional Characterization of Apolipoproteins in the HCV Life Cycle -- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes -- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins -- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis -- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells -- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact -- Complement Regulation and Immune Evasion by Hepatitis C Virus -- Direct ex vivo Functional Analysis of HCV-specific T Cells -- ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry -- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display -- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA -- In vitro Neutralization Assay using Cultured Hepatitis C Virus -- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp) -- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice -- Mice with Chimeric Human Livers and Their Applications -- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies
    Abstract: This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIX, 508 p. 75 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989768
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  • 6
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 7
    Cham : Springer
    Call number: QZ200:576 ; C100:50
    Pages: vii, 205 p. : ill.
    ISBN: 9783030012069
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    QZ200:576 available
    C100:50 departmental collection or stack – please contact the library
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  • 8
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 9
    Keywords: Biomedical Engineering ; Neurosciences ; Radiology, Medical ; Neurology ; Biomedical Engineering/Biotechnology ; Neurosciences ; Diagnostic Radiology ; Mathematical and Computational Biology ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Introduction -- Chapter 2. Multifractal study of EEG signal of subjects with Epilepsy and Alzheimer’s -- Chapter 3. Multifractal Approach for Quantification of Autonomic Deregulation due to Epileptic Seizure with ECG data -- Chapter 4. Multifractal Analysis of Electromyography data.-Chapter 5. Multifractal study on Parkinson’s and Huntington diseases with human gait data -- Chapter 6. Multifractal correlation study between posture and autonomic deregulation using ECG and blood pressure data -- Chapter 7. Appendix
    Abstract: This book primarily focuses on the study of various neurological disorders, including Parkinson’s (PD), Huntington (HD), Epilepsy, Alzheimer’s and Motor Neuron Diseases (MND) from a new perspective by analyzing the physiological signals associated with them using non-linear dynamics. The development of nonlinear methods has significantly helped to study complex nonlinear systems in detail by providing accurate and reliable information. The book provides a brief introduction to the central nervous system and its various disorders, their effects on health and quality of life, and their respective courses of treatment, followed by different bioelectrical signals like those detected by Electroencephalography (EEG), Electrocardiography (ECG), and Electromyography (EMG). In turn, the book discusses a range of nonlinear techniques, fractals, multifractals, and Higuchi’s Fractal Dimension (HFD), with mathematical examples and procedures. A review of studies conducted to date on neurological disorders like epilepsy, dementia, Parkinson’s, Huntington, Alzheimer’s, and Motor Neuron Diseases, which incorporate linear and nonlinear techniques, is also provided. The book subsequently presents new findings on neurological disorders of the central nervous system, namely Parkinson’s disease and Huntington’s disease, by analyzing their gait characteristics using a nonlinear fractal based technique: Multifractal Detrended Fluctuation Analysis (MFDFA). In closing, the book elaborates on several parameters that can be obtained from cross-correlation studies of ECG and blood pressure, and can be used as markers for neurological disorders
    Pages: XX, 182 p. 77 illus., 51 illus. in color. : online resource.
    ISBN: 9789811335525
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  • 10
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- A Neuron-Glial perspective for computational neuroscience -- Part 2. Calcium dynamics -- Data-driven modelling of the inositol trisphosphate receptor and its role in calcium induced calcium release -- Intracellular calcium dynamics: biophysical and simplified models -- Modeling of stochastic Ca2+ signals -- G protein-coupled receptor-mediated astrocytic Ca2+ signaling in astrocytes -- Emergence of regular and complex calcium oscillations by inositol 1,4,5-trisphosphate signaling in astrocytes -- Astrocyte networks and intercellular calcium propagation -- Part 3. Tripartite Synapse and regulation of network activity -- Tripartite synapses and regulation of synaptic plasticity -- Purinergic signaling at tripartite synapses -- Gliotransmitter exocytosis and its consequences on synaptic transmission -- Computational models of pathophysiological glial activation in CNS disorders -- Part 4. Homeostasis and Metabolic coupling -- The role of astrocytes in neurotransmitter uptake and brain metabolism -- Glutamate uptake -- Astrocytic ion dynamics: Implications for potassium buffering and liquid flow -- Constraint-Based Modeling of Metabolic Interactions in and between Astrocytes and Neurons -- Part 5. Computational Tools to Analyze and Model Astrocyte Experiments -- Computational models of astrocytes and astrocyte-neuron interactions: Characterization, reproducibility, and future perspectives -- GECIquant: semi-automated detection and quantification of astrocyte intracellular Ca2+ signals monitored with GCaMP6f -- Modeling neuron-glia interactions with the Brian 2.0 simulator
    Abstract: Over the last two decades, the recognition that astrocytes - the predominant type of cortical glial cells - could sense neighboring neuronal activity and release neuroactive agents, has been instrumental in the uncovering of many roles that these cells could play in brain processing and the storage of information. These findings initiated a conceptual revolution that leads to rethinking how brain communication works since they imply that information travels and is processed not just in the neuronal circuitry but in an expanded neuron-glial network. On the other hand the physiological need for astrocyte signaling in brain information processing and the modes of action of these cells in computational tasks remain largely undefined. This is due, to a large extent, both to the lack of conclusive experimental evidence, and to a substantial lack of a theoretical framework to address modeling and characterization of the many possible astrocyte functions. This book that we propose aims at filling this gap, providing the first systematic computational approach to the complex, wide subject of neuron-glia interactions. The organization of the book is unique insofar as it considers a selection of ́€œhot topicś€ in glia research that ideally brings together both the novelty of the recent experimental findings in the field and the modelling challenge that they bear. A chapter written by experimentalists, possibly in collaboration with theoreticians, will introduce each topic. The aim of this chapter, that we foresee less technical in its style than in conventional reviews, will be to provide a review as clear as possible, of what is €œéstablished́€ and what remains speculative (i.e. the open questions). Each topic will then be presented in its possible different aspects, by 2-3 chapters by theoreticians. These chapters will be edited in order to provide a ́€œpriminǵ€ reference for modeling neuron-glia interactions, suitable both for the graduate student and the professional researcher
    Pages: XV, 505 p. 205 illus., 76 illus. in color. : online resource.
    ISBN: 9783030008178
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  • 11
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Normal Growth in PKU Patients Under Low-Protein Diet in a Single-Center Cross-Sectional Study -- Serial Magnetic Resonance Imaging and 1H-Magnetic Resonance Spectroscopy in GABA Transaminase Deficiency: A Case Report.-Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment -- Cognitive and Behavioural Outcomes of Paediatric Liver Transplantation for Ornithine Transcarbamylase Deficiency -- Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation -- Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice -- Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period -- Severe Leukoencephalopathy with Clinical Recovery Caused by Recessive BOLA3 Mutations -- Neonatal Onset Interstitial Lung Disease as a Primary Presenting Manifestation of Mucopolysaccharidosis Type I -- A Middle Eastern Founder Mutation Expands the Genotypic and Phenotypic Spectrum of Mitochondrial MICU1 Deficiency: A Report of 13 Patients -- Disruption of the Responsible Gene in a Phosphoglucomutase 1 Deficiency Patient by Homozygous Chromosomal Inversion -- Evaluation of Disease Lesions in the Developing Canine MPS IIIA Brain -- Extrapolation of Variant Phase in Mitochondrial Short-Chain Enoyl-CoA Hydratase (ECHS1) Deficiency -- RFT1-CDG: Absence of Epilepsy and Deafness in Two Patients with Novel Pathogenic Variants -- Short-Term Administration of Mycophenolate Is Well-Tolerated in CLN3 Disease (Juvenile Neuronal Ceroid Lipofuscinosis)
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 35 illus., 20 illus. in color. : online resource.
    ISBN: 9783662586143
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  • 12
    Keywords: Ecology ; Evolution (Biology) ; Plant Ecology ; Animal ecology ; Plant genetics ; Biodiversity ; Community & Population Ecology ; Evolutionary Biology ; Plant Ecology ; Animal ecology ; Plant Genetics and Genomics ; Biodiversity ; Springer eBooks
    Description / Table of Contents: Part1. Concepts, Technical Advances and Challenges -- Chapter1. Technical Advances and Challenges in Genome-Scale Analysis of Ancient DNA -- Chapter2. Paleoproteomics: An Introduction to the Analysis of Ancient Proteins by Soft Ionization Mass Spectrometry -- Chapter3. Ancient RNA -- Chapter4. Ancient Epigenomics -- Part2. -- Chapter5. Ancient Pathogens Through Human History: A Paleogenomic Perspective -- Chapter6. Paleovirology: Viral Sequences from Historical and Ancient DNA -- Chapter7. Reconstructing Past Vegetation Communities Using Ancient DNA from Lake Sediments -- Chapter8. Archaeogenomics and Crop Adaptation -- Chapter9. Herbarium Genomics: Plant Archival DNA Explored -- Chapter10. Paleogenomics of Animal Domestication -- Chapter11. Paleogenomic Inferences of Dog Domestication -- Chapter12. Of Cats and Men: Ancient DNA Reveals How the Cat Conquered the Ancient World -- Chapter13. An Ancient DNA Perspective on Horse Evolution -- Chapter14. Primate Paleogenomics -- Chapter15. Structural Variants in Ancient Genomes -- Chapter16. Genomics of Extinction
    Abstract: Advances in genome-scale DNA sequencing technologies have revolutionized genetic research on ancient organisms, extinct species, and past environments. When it is recoverable after hundreds or thousands of years of unintended preservation, €œáncient DNÁ€ (or aDNA) is often highly degraded, necessitating specialized handling and analytical approaches. Paleogenomics defines the field of reconstructing and analyzing the genomes of historic or long-dead organisms, most often through comparison with modern representatives of the same or similar species. The opportunity to isolate and study paleogenomes has radically transformed many fields, spanning biology, anthropology, agriculture, and medicine. Examples include understanding evolutionary relationships of extinct species known only from fossils, the domestication of plants and animals, and the evolution and geographical spread of certain pathogens. This pioneering book presents a snapshot view of the history, current status, and future prospects of paleogenomics, taking a broad viewpoint that covers a range of topics and organisms to provide an up-to-date status of the applications, challenges, and promise of the field. This book is intended for a variety of readerships, including upper-level undergraduate and graduate students, professionals and experts in the field, as well as anyone excited by the extraordinary insights that paleogenomics offers
    Pages: XIV, 427 p. 62 illus., 57 illus. in color. : online resource.
    ISBN: 9783030047535
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  • 13
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 14
    Heidelberg : Springer
    Call number: QH442:222 ; ERW
    Keywords: Genetic Techniques
    ISBN: 9783642346934
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    QH442:222 available
    ERW in acquisition
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  • 15
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 16
    Keywords: Biological models ; Proteomics ; Gene Expression ; Systems Biology ; Proteomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: 1. The Potential of €˜Ómics to Link Lipid Metabolism and Genetic and Comorbidity Risk Factors of Alzheimeŕ€™s Disease in African Americans -- 2. The Role of Biomarkers in Alzheimeŕ€™s Disease Drug Development -- 3. Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- 4. Lymphocytes, Platelets, Erythrocytes and Exosomes as Possible Biomarkers for Alzheimer's Disease Clinical Diagnosis -- 5. Genetic Risk Factors for Alzheimer Disease: Emerging Roles of Microglia in Disease Pathomechanisms -- 6. Neuroimaging Studies of Cognitive Function in Schizophrenia -- 7. The Role of Biomarkers in Psychiatry -- 8. Interactome Studies of Psychiatric Disorders -- 9. MicroRNAs in Major Depressive Disorder -- 10. Proteomic Markers for Depression -- 11. Advances in Biomarker Studies in Autism Spectrum Disorders -- 12. Proteomic Investigations of Autism Spectrum Disorder: Past Findings, Current Challenges and Future Prospects -- 13. Role of the Gut Microbiome in Autism Spectrum Disorders -- 14. Metabolomic Biomarkers in Mental Disorders: Bipolar Disorder and Schizophrenia -- 15. Early Detection and Treatment of Patients with Alzheimeŕ€™s Disease: Future Perspectives
    Abstract: This book includes a series of reviews on general aspects of biomarker use in the study of psychiatric and neurodegenerative diseases and the development of medications involved in their treatment. It describes the pros and cons of the various approaches and covers the successes and failures in this research field. It is only by a thorough understanding of the shortcomings that progress can be made. The overall goal is to facilitate the understanding and treatment of these disorders, by providing a viable mechanism of catching up with other areas of modern medicine, such as diabetes and heart disease. Finally, it is anticipated that the development and application of valid biomarker tests and the leveraging of novel drug targets will help the fields of psychiatry on neurodegenerative disorders move into the area of personalized medicine where the right patients can receive the right medication at the right time for the best possible outcome
    Pages: XIII, 328 p. 28 illus., 26 illus. in color. : online resource.
    ISBN: 9783030055424
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  • 17
    Keywords: Immunology ; Immunology ; Springer eBooks
    Description / Table of Contents: Isolation and Characterisation of Human Adipose-derived Stem Cells -- Isolation of Murine Hematopoietic Stem Cells -- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells -- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting -- Identification and isolation of Regulatory B cells in Mouse and Human -- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis -- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells -- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade -- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells -- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays -- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments -- Immune tolerance and rejection in organ transplantation -- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation -- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood -- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental -- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
    Abstract: This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 240 p. 43 illus., 36 illus. in color. : online resource.
    ISBN: 9781493989386
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  • 18
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 19
    Keywords: Nucleic Acids ; Genetic Engineering ; Popular Life Sciences ; Nucleic Acid Chemistry ; Genetic Engineering ; Popular Science in Chemistry and Materials ; Springer eBooks
    Abstract: Carolyń€™s parents did not, after all, make genomics history by synthesizing her genome in a lab. She has known she is the "Human Hoax" ever since a high school genetics exercise revealed she has trisomy X€”á chromosomal abnormality€”ýet no synthetically constructed genome would have such clear traces of natural conception. Many years later, as molecular biologist, she hopes her colleagues never learn of her embarrassing origins. But when someone ransacks her office and lab, she finds professional embarrassment is the least of her worries. Someone believes she has the results of her parentś€™ last, secret experiments, and is willing to kill to get them. But all she has from her parents are their geneś€”can she find what else they may have left her before somebody else does? In a not-so-distant society, where corporations wield as much power as nations and the line between corporate employee and state authority is blurred, the chase is on. Carolyn may have just too little time at hand to unravel the mystery of her parentś€™ final days and to realize the deep consequences for the future of mankind. This fast-paced novel is followed by an extensive science chapter where the author provides a non-technical primer on modern genetics and on the speculative biology behind Carolyń€™s code. Dr V Anne Smith is on the biology faculty at the University of St Andrews in Scotland. She researches computational biology, concentrating on complex biological networks. She develops algorithms to infer functional interactions based upon observational data, generating biological discovery in areas as diverse as ecosystems and gene regulation. In addition to doing research, she enjoys writing science fiction stories. A Code for Carolyn is her first scientific novel
    Pages: V, 235 p. 6 illus. : online resource.
    ISBN: 9783030045531
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  • 20
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: A Genomic View of the Catabolism of Aromatic Compounds in Pseudomonas -- A Genomic View of the Catabolism of Short Chain Alkanes -- Aerobic Degradation of Aromatic Hydrocarbons -- Aerobic Degradation of Aromatic Hydrocarbons: Enzyme Structures and Catalytic Mechanisms -- Aerobic Degradation of Chloroaromatics -- Aerobic Degradation of Gasoline Ether Oxygenates -- Aerobic Degradation of Halogenated Aliphatics -- Biochemistry and Molecular Biology of Methane Monooxygenase -- Biosynthesis, Insertion and Function of Fe-S Cofactors -- Biosynthesis, Insertion and Function of Flavin Cofactors -- Biosynthesis, Insertion and Function of haem-iron Prosthetic Groups -- Computational Framework for Integration of Lipidomics Data into Metabolic Pathways -- Degradation of Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) -- Degradation of Rubber/Polyisoprene -- Diversity and Common Principles in Enzymatic Activation of Hydrocarbons: An Introduction -- Energetic and Other Quantitative Aspects of Microbial Hydrocarbon Utilization: An Introduction: -- Enzymes for Aerobic Degradation of Alkanes in Bacteria -- Enzymes for Aerobic Degradation of Alkanes in Yeasts -- Evolution of New Catabolic Functions Through Gene Assembly by Mobile Genetic Elements -- Experimental Evolution of Novel Regulatory Activities in Response to Hydrocarbons and Related Chemicals -- Functional Gene Diversity, Biogeography, Dynamics -- Genetic Features and Regulation of n-Alkane Metabolism in Bacteria -- Genetic Features and Regulation of n-Alkane Metabolism in Yeasts -- Genetic Features of Methylotrophs -- Genetics and Functional Genomics of Aerobic Degradation of Hydrocarbons: An Introduction -- Genetics and Molecular Features of Bacterial Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) Transformations -- Genetics of Biphenyl Biodegradation and Co-Metabolism of PCBs -- Genetics of Rubber/Polyisoprene Degradation -- Genetics and Ecology of Isoprene Degradation -- Genomic View of Mycobacterial High Molecular Weight Polycyclic Aromatic Hydrocarbon Degradation -- Global Aerobic Degradation of Hydrocarbons in Aquatic Systems -- Global Aerobic Degradation of Hydrocarbons in Terrestrial Systems -- Global Consequences of the Microbial Production and Consumption of Inorganic and Organic Sulfur Compounds -- Key Features of Aerobic Hydrocarbon Biodegrader Genomes -- Lipid Degradation - Lipid Cycles -- Lipolytic Enzymes from Bacteria -- Lipolytic Enzymes from Yeasts -- Metabolism of Steroids -- Oxidative Inactivation of Ring-Cleavage Extradiol Dioxygenases: Mechanism and Ferredoxin-Mediated Reactivation -- Pathways for the Degradation of Fatty Acids in Bacteria -- Phylogenomics of Aerobic Bacterial Degradation of Aromatics -- Physiology and Biochemistry of the Aerobic Methane Oxidizing Bacteria -- Potential for Microbial Interventions to Reduce Global Warming -- Rational Construction of Bacterial Strains with New/Improved Catabolic Capabilities for the Efficient Breakdown of Environmental Pollutants -- Regulation of the Degradation of Fatty Acids in Bacteria -- Regulation of the Degradation of Fatty Acids in Yeast -- Stereochemistry of Hydrocarbon-transforming Enzyme Reactions -- Structure-Function Relationships and Engineering of Haloalkane Dehalogenases -- Transcriptional Control of the TOL Plasmid Pathways
    Abstract: This book describes the biochemistry and genetics of aerobic degradation of hydrocarbons and lipids by bacteria and yeasts, as well as the functional genomics of the best-known microorganisms involved in these processes. It forms part of the Handbook of Hydrocarbon and Lipid Microbiology Series, a definitive resource of current knowledge on the diverse aspects of the interactions of microbes with hydrocarbons and lipids. Chapters are mainly focused on the new discoveries of recent years. It is aimed to scientists and others interested in different aspects of the microbiology of hydrocarbons
    Pages: 149 illus., 43 illus. in color. eReference. : online resource.
    ISBN: 9783319504186
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  • 21
    Keywords: Plant genetics ; Gene Expression ; Plant Genetics and Genomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants -- The Plant MicroRNA Pathway: The Production and Action Stages -- Plant miRNA Conservation and Evolution -- Bioinformatic Analysis of Small RNA Sequencing Libraries -- An Overview of miRNA and miRNA Target Analysis Tools -- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences -- Prediction of Plant miRNA Targets -- Isolation and Detection Methods of Plant miRNAs -- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants -- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses -- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization -- In Situ Localization of Small RNAs in Plants -- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs -- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy -- Degradome Sequencing in Plants -- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System -- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis -- Mimicry Technology: A Versatile Tool for Small RNA Suppression -- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants -- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches -- Analysis of Methylation Status of Plant MicroRNAs -- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 -- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs -- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae -- Detection of Histone Modifications Associated with miRNAs
    Abstract: This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science
    Pages: XII, 363 p. 70 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990429
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  • 22
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Physiological Characterization of Phototropism in Arabidopsis Seedlings -- Assessing Negative and Positive Phototropism in Lianas -- Determination of Phototropism and Polarotropism in Fern Protonemal Cells -- Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli -- An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens -- Observation of Phototropic Responses in the Liverwort Marchantia polymorpha -- Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus -- Quantitative Analysis of Tip Growth, Phototropic Responses and Other Blue Light-Dependent Photoresponses of Vaucheria -- A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezonensis -- Determination of Phototropism by UV-B Radiation -- New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport -- Determination of Auxin Flow during Phototropic Responses Using Fluorescent Auxin Analogs -- Immunolocalization of IAA Using an Anti-IAA-C Antibody Raised against Carboxyl-Linked IAA -- Measurements of Photoreaction and Kinase Activity of Phototropin, a Photoreceptor Protein for Tropic Response in Plants; Involvement of Kinase Activity in the Photosensitivity of Tropic Response -- Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema -- Grafting as a Tool for Study of Phototropism in Phycomyces -- Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station -- How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening -- Quantitative Measurements of Curvature along the Growth Axis in Tropic Responses Using Free Software Environments
    Abstract: This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin. In addition, the use of grafting, spaceflight experiment and image analysis in tropism study is provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phototropism: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: X, 240 p. 89 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990153
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  • 23
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction -- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction -- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells -- Isolation and Characterization of Cutaneous Epithelial Stem Cells -- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin -- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells -- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis -- Isolation and Cultivation of Epidermal (Stem) Cells -- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen -- Isolation and Cultivation of Skin-Derived Precursors -- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells -- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor -- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair -- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet -- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering -- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration -- Skin Wound Healing: Refractory Wounds and Novel Solutions -- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice -- Melanoblasts as Multipotent Cells in Murine Skin -- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro -- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells -- Isolating Immune Cells from Mouse Embryonic Skin -- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells -- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy -- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications -- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis -- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells -- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research -- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma -- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells -- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
    Abstract: This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells
    Pages: XV, 463 p. 105 illus., 76 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988709
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  • 24
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels -- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling -- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators -- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes -- MCU Regulation in Lipid Bilayer and Electrophysiological Recording -- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer -- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter -- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana -- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes -- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes -- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle -- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis -- Ca2+-Dependent Regulation of TFEB and Lysosomal Function -- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum -- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2 -- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes -- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes -- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer -- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells -- Purification of Functional F-ATP Synthase from Blue Native PAGE
    Abstract: This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research
    Pages: XIII, 246 p. 48 illus., 39 illus. in color. : online resource.
    ISBN: 9781493990184
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  • 25
    Keywords: Bioinformatics ; Human Genetics ; Bioinformatics ; Human Genetics ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: ncRNAs in Inflammatory and Infectious Diseases -- p73-Governed miRNA Networks: Translating Bioinformatics Approaches to Therapeutic Solutions for Cancer Metastasis -- Methods for Annotation and Validation of Circular RNAs from RNAseq Data -- Methods to Study Long Non-coding RNA Expression and Dynamics in Zebrafish using RNA Sequencing -- Workflow Development for the Functional Characterization of ncRNAs -- ncRNA Editing: Functional Characterization and Computational Resources -- Computational Prediction of Functional microRNA – mRNA Interactions -- Tools for Understanding miRNA-mRNA Interactions for Reproducible RNA Analysis -- Computational Resources for Prediction and Analysis of Functional miRNA and their Targetome -- Non-coding RNAs Databases: Current Status and Trends -- Controllability Methods for Identifying Associations between Critical Control ncRNAs and Human Diseases -- Network-based Methods and Other Approaches for Predicting lncRNA Functions and Disease Associations -- Integration of miRNA and mRNA Expression Data for Understanding Etiology of Gynecologic Cancers -- Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks -- Kinetic Modeling of Competition and Depletion of Shared miRNAs by Competing Endogenous RNAs -- Modeling ncRNA-mediated Circuits in Cell Fate Decision -- Modeling Long ncRNA-mediated Regulation in the Mammalian Cell Cycle
    Abstract: This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field
    Pages: XII, 451 p. 78 illus., 65 illus. in color. : online resource.
    ISBN: 9781493989829
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  • 26
    Keywords: Pharmaceutical technology ; Chromatography ; Chemical engineering ; Pharmaceutical Sciences/Technology ; Chromatography ; Industrial Chemistry/Chemical Engineering ; Springer eBooks
    Description / Table of Contents: Separation and Purification of Amino acids -- Ion exchange chromatography for enzyme immobilization -- Determination of morphine in urine -- Chromatographic Separation of Amino Acids -- Applications of ion exchange chromatography in pharmaceutical analysis -- Thermodynamic Kinetics and Sorption of Bovine Serum Albumin with Different Clay materials -- Sorbitol demineralization by ion exchange -- Separation and purification of nucleotides, nucleosides, purine and pyrimidine bases by ion exchange -- Separation and purification of vitamins: vitamin B1, B2, B6, C, and K1 -- Colour removal from sugar syrups
    Abstract: This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists
    Pages: XII, 225 p. 41 illus., 29 illus. in color. : online resource.
    ISBN: 9783030060824
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  • 27
    Keywords: Evolution (Biology) ; Life sciences ; Environmental sciences-Philosoph ; Biology-Philosophy ; Philosophy of nature ; Ecology ; Evolutionary Biology ; Popular Science in Nature and Environment ; Environmental Philosophy ; Philosophy of Biology ; Philosophy of nature ; Ecology ; Springer eBooks
    Description / Table of Contents: Introdution -- Between the Theoretical and the Hypothetical -- Taxonomic Uncertainties -- A Biosphere in Flux -- As Far as the Microscope Reveals -- Human Contradictions -- Varieties of Social Contracts -- Biological Consensus Mechanisms: The Future of Co-Existence
    Abstract: This book is a provocative and invigorating real-time exploration of the future of human evolution by two of the world’s leading interdisciplinary ecologists – Michael Charles Tobias and Jane Gray Morrison. Steeped in a rich multitude of the sciences and humanities, the book enshrines an elegant narrative that is highly empathetic, personal, scientifically wide-ranging and original. It focuses on the geo-positioning of the human Self and its corresponding species. The book's overarching viewpoints and poignant through-story examine and powerfully challenge concepts associated historically with assertions of human superiority over all other life forms. Ultimately, The Hypothetical Species: Variables of Human Evolution is a deeply considered treatise on the ecological and psychological state of humanity and her options – both within, and outside the rubrics of evolutionary research – for survival. This important work is beautifully presented with nearly 200 diverse illustrations, and is introduced with a foreword by famed paleobiologist, Dr. Melanie DeVore
    Pages: X, 318 p. 225 illus., 205 illus. in color. : online resource.
    ISBN: 9783030113193
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  • 28
    Keywords: Bioinformatics ; Genetics ; Human Genetics ; Agriculture ; Bioinformatics ; Genetics and Genomics ; Human Genetics ; Agriculture ; Springer eBooks
    Description / Table of Contents: Introduction to biological data -- Computers and bioinformatics -- Sequence biological databases -- Biological databases -- Other biological databases -- Nucleic acid sequencing -- Tools and methods in analysis of simple sequences -- Tools and methods in analysis of complex sequences -- Structural bioinformatics -- Protein structure annotations -- Introduction to functional bioinformatics -- Biological networks – tools, methods and analysis -- Metabolomics -- Drug discovery: concepts and approaches -- Drug designing methods and analysis -- Molecular molecular docking -- Insilico pcr -- Modeling and optimization of molecular bio-systems to generate predictive models -- Index
    Abstract: Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it mostly focuses on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. Unlike other bioinformatics books which are mostly theoretical, this book provides practical examples for the readers on state-of-the-art open source tools to solve biological problems. Flow charts of experiments, graphical illustrations, and mock data are included for quick reference. Volume I is therefore an ideal companion for students and early stage professionals wishing to master this blooming field
    Pages: XIV, 405 p. 193 illus., 186 illus. in color. : online resource.
    ISBN: 9783030026349
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  • 29
    Keywords: Medical genetics ; Medicine ; Biochemistry ; Neurosciences ; Gene Function ; Molecular Medicine ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Chapter 1. Molecular and Functional Properties of Progranulin -- Chapter 2. Progranulin as a biomarker for neurodegenerative diseases -- Chapter 3. PGRN and FTLD -- Chapter 4. PGRN and neurodegenerative diseases other than FTLD -- Chapter 5. Progranulin Regulations of Lysosomal Homeostasis and its Involvement in Neurodegenerative Diseases -- Chapter 6. Molecular and Functional Properties of Progranulin -- Chapter 7. PGRN and neuroinflammation -- Chapter 8. Neural Stem/Progenitor Cells and Progranulin -- Chapter 9. Generation and phenotyping of progranulin-deficient mice -- Chapter 10. Pleiotropic protective effects of progranulin in the treatment of ischemic stroke -- Chapter 11. New therapeutic approaches against ocular diseases
    Abstract: This book presents the latest knowledge on the roles of progranulin (PGRN) in normal physiology and pathology and explores the emerging significance of PGRN as a therapeutic target and biomarker in various CNS disorders, including frontotemporal lobe degeneration and other neurodegenerative diseases. Following initial recognition of the importance of PGRN in sexual differentiation of the developing brain and adult neurogenesis, it was subsequently discovered that PGRN acts as a chaperone of lysosomal enzymes and plays a crucial role in maintaining cellular protein homeostasis. It has also been found that sex steroids modulate the expression of PGRN and its trophic effects in the developing CNS and that PGRN directly or indirectly influences neural stem and progenitor cells. Against this background, deeper understanding of the molecular and functional properties of PGRN would provide fresh impetus for the development of mechanism-based therapeutic approaches for multiple disorders. Medications targeting the recovery of lysosomal function appear to hold particular promise in patients with neurodegenerative diseases resulting from PGRN insufficiency. In examining multiple aspects of this fascinating field, the book will be of high value for researchers and graduate students
    Pages: VII, 183 p. 37 illus., 13 illus. in color. : online resource.
    ISBN: 9789811361869
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  • 30
    Keywords: Medical laboratories ; Pharmaceutical technology ; Laboratory Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Part1.Introduction -- 1.1 Rules of laboratory safety in a pharmacology laboratory -- 1.2 Commom instruments and their uses used in a pharmacology laboratory -- Part2.General aspects of Pharmacology laboratory -- 2.1 Safety and Risk assessment -- 2.2 Use and Handling of laboratory animals -- 2.3 Experimental design -- 2.4 Essential statistics -- 2.5 Cumulative Dose Response Curve -- 2.6 Toxicology -- 2.7 Basic instruments and techniques in Pharmacology Laboratory -- Part3.Isolated tissues and organs -- 3.1 Basic instruments used for isolated tissue experiments -- 3.2 Organ baths -- 3.3 Smooth muscle preparations -- 3.4 Skeletal muscle preparations -- 3.5 Cardiac muscle preparations -- Part4.Isolated tissues for screening of drugs -- 4.1 Evaluation of antidiabetic agents -- 4.2 Evaluation of antidepressants -- 4.3 Evaluation of antihypertensive agents -- 4.4 Evaluation of antiulcer agents -- 4.5 Evaluation of hepatotoxicity -- 4.6 Evaluation of Antioxidant agents -- 4.7 Evaluation of local anaesthetics -- Part5.Genotoxicity and Toxicological studies -- 5.1 The Mouse Lymphoma Assay -- 5.2 The Comet Assay -- 5.3 In vitro Genotoxicity assay -- 5.4 In vitro Teratogenicity Testing -- 5.5 Histopathological studies of animal tissues -- 5.6 Drug poisoning -- Part6.Experimental Animal studies -- 6.1 Pyrogen testing -- 6.2 Collecting blood from mice -- 6.3 Studies on different parameters of blood -- 6.4 Experiment on central nervous system -- 6.5 Evaluation on cardiovascular system -- 6.6 Experiments on GI tract -- Part7.Clinical trials -- 7.1 Clinical Pharmacology and its genesis -- 7.2 National and International agencies and their role in Clinical pharmacology -- 7.3 Stages in drug development and clinical trials -- 7.4 Ethics in Clinical research -- 7.5 Safety assessment in Clinical trials -- Part8.IPR and ethics in animal studies -- 8.1 Intellectual property rights and its different categories -- 8.2 Importance of IPR in drug development -- 8.3 Patenting cells, cell lines and animals -- 8.4 Ethics in laboratory animal studies -- 8.5 Risk assessment and management -- 8.6 Good laboratory practices
    Abstract: Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This manual is the textbook covering the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory
    Pages: XIII, 142 p. 50 illus., 39 illus. in color. : online resource.
    ISBN: 9789811363559
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  • 31
    Keywords: Microbiology ; Microbial genetics ; Microbial genomics ; Biomedical Engineering ; Eukaryotic Microbiology ; Microbial Genetics and Genomics ; Applied Microbiology ; Medical Microbiology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Industrial Applications and Model Systems -- Physiological genomics of multistress resistance in the yeast cell model and factory: focus on MDR/MXR transporters -- Mechanisms of yeast adaptation to wine fermentations -- Development of robust yeast strains for lignocellulosic bio-refineries based on genome-wide studies -- Physiological genomics of the highly weak-acid-tolerant food spoilage yeasts of Zygosaccharomyces bailii sensu lato -- Yeast genome-scale metabolic models for simulating genotype-phenotype relations -- Human Health and Disease -- Emerging mechanisms of drug resistance in Candida albicans -- Genome-wide response to drugs and stress in the pathogenic yeast Candida glabrata -- Lipidomics approaches: applied to the study of pathogenesis in Candida species -- Yeast at the forefront of research on ageing and age-related diseases
    Abstract: This book discusses genome-based strategies to provide a holistic understanding of yeasts in Human Health and as model organisms in basic research or industrial production. Using numerous Saccharomyces cerevisiae strains and various non-conventional yeast species isolated from diverse origins, it describes essential biological processes, the biotechnological exploitation of yeast and pathogenesis control. It also demonstrates how functional and comparative genomics and the development of genome engineering tools are used in modern yeast research. The use of yeasts as experimental eukaryotic models increasingly gained prominence when several Nobel Prizes in Physiology/Medicine and Chemistry were awarded for innovative research, using yeast strains to elucidate molecular mechanisms in a wide range of human physiological processes and diseases, such as autophagy, cell cycle regulation and telomerase activity. This book offers useful insights for scientists in yeast research, clinical scientists working with yeast infectious models and for industrial researchers using applied microbiology
    Pages: V, 242 p. 37 illus., 29 illus. in color. : online resource.
    ISBN: 9783030130350
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  • 32
    Keywords: Entomology ; Plant breeding ; Plant anatomy ; Biomedical Engineering ; Mycology ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Preface -- Biodiversity of Fungi from Extreme Habitats and Its Industrial Applications -- Rhizospheric Fungi Isolated from Different Plants and Potential Applications in Diverse Sectors -- Endophytic Fungi from Different Niches with Potential Industrial Importance -- Marine Fungal White Biotechnology -- Aspergillus Biodiversity, Ecological Significance, and Industrial Applications -- Fusarium Biodiversity, Ecological Significance, and Industrial Applications -- Penicillium Biodiversity, Ecological Significance, and Industrial Applications -- Trichoderma Biodiversity, Ecological Significance, and Industrial Applications -- Thermostable Enzymes from Fungi Isolated from Hot Springs and Its Applications -- Cold Active Enzymes from Psychrophilic Fungi and Its Applications -- Industrially Important Enzymes from White-Rot Fungi or Filamentous Fungi -- Fungal Enzymes for the Textile Industry -- Fungal Laccase -- Fermentation Process for Production of Fungal Cellulases -- Fungal Lipases -- Fungal Community and Eco-Friendly Bioresources for Novel Secondary Metabolites -- Agriculturally Important Fungi -- Fungal White Biotechnology in Biosurfactants -- Synthetic Biology and a Novel Approach for Pharmaceutically Important Compounds -- Global Scenario of Fungal White Biotechnology -- Appendixes -- Index --
    Abstract: White biotechnology, or industrial biotechnology as it is also known, refers to the use of living cells and/or their enzymes to create industrial products that are more easily degradable, require less energy, create less waste during production and sometimes perform better than products created using traditional chemical processes. Over the last decade considerable progress has been made in white biotechnology research, and further major scientific and technological breakthroughs are expected in the future. Fungi are ubiquitous in nature and have been sorted out from different habitats, including extreme environments (high temperature, low temperature, salinity and pH), and may be associated with plants (epiphytic, endophytic and rhizospheric). The fungal strains are beneficial as well as harmful for human beings. The beneficial fungal strains may play important roles in the agricultural, industrial, and medical sectors. The fungal strains and their products (enzymes, bioactive compounds, and secondary metabolites) are very useful for industry (e.g., the discovery of penicillin from Penicillium chrysogenum). This discovery was a milestone in the development of white biotechnology as the industrial production of penicillin and antibiotics using fungi moved industrial biotechnology into the modern era, transforming it into a global industrial technology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors, providing both high value nutraceutical and pharmaceutical products. The fungal strains and bioactive compounds also play an important role in environmental cleaning. This volume covers the latest developments and research in white biotechnology with a focus on diversity and enzymes
    Pages: XIX, 571 p. 108 illus., 61 illus. in color. : online resource.
    ISBN: 9783030104801
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  • 33
    Keywords: Stem Cells ; Ophthalmology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Ophthalmology ; Springer eBooks
    Description / Table of Contents: Advantages of the Eye as a Target Organ for Cell-Based Therapy in the Central Nervous System -- Proof of Principle: Preclinical Data on Retinal Cell Transplantation -- Clinical and Pathological Features of Selected Human Retinal Degenerative Diseases -- Degenerative Retinal Diseases: Cells sources for cell-based therapy -- Manufacturing of Clinical Grade Cellular Products Under Gmp Conditions -- Regulatory Requirements for Cell-Based Therapy for Degenerative Retinal Disease -- Biomaterials and Scaffolds for Cell Replacement Therapy -- Restoration of cone photoreceptor function in retinitis pigmentosa (RP) – retinal cell-based therapy -- Cell Delivery: Surgical Approaches -- Diagnosis and Complementary Examinations -- Cell Therapy for Degenerative Retinal Disease: special focus on cell fusion-mediated regeneration -- Clinical Trials of Retinal Cell Therapy -- Future Directions -- Index
    Abstract: This book discusses why specific diseases are being targeted for cell-based retinal therapy, what evidence exists that justifies optimism for this approach, and what challenges must be managed in order to bring this technology from the laboratory into routine clinical practice. There are a number of unanswered questions (e.g., surgical approach to cell delivery, management of immune response, optimum cell type to transplant) that very likely are not going to be answered until human trials are undertaken, but there is a certain amount of “de-risking” that can be done with preclinical experimentation. This book is essential reading for scientists, clinicians, and advanced students in stem cell research, cell biology, and ophthalmology
    Pages: XI, 280 p. 27 illus., 20 illus. in color. : online resource.
    ISBN: 9783030052225
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  • 34
    Singapore : Springer
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Evolution (Biology) ; Biodiversity ; Drug Interactions ; Microbial Genetics and Genomics ; Microbial ecology ; Evolutionary Biology ; Biodiversity ; Drug Resistance ; Springer eBooks
    Description / Table of Contents: Bacteria -- Functions of archaeal nucleoid proteins: archaeal silencers are still missing -- Acquired and innate immunity in prokaryote define their evolutionary story -- Mobile genetic elements -- Viruses of the archaea and evolution of life -- Overlooked “broad-host range vector particles” (VPs) in the environ -- Bacteria-virus interaction -- Plasmids and their hosts -- Bacterial transposable elements -- RNA-mediated crosstalk between bacterial host genome and foreign genetic elements -- Extracellular DNA in seawater and marine sediment -- Acquiring phenotypic diversification on genomic diversification
    Abstract: This book comprehensively discusses our current understanding of the role and biological mechanisms of horizontal transfer of genetic elements in the environment, which has been important in the evolution of prokaryotes (archaea and bacteria). Horizontal transfer of genetic elements generates variations of prokaryotes and their genomes. Comparative studies of genomes revealed that it frequently occurred during archaeal and bacterial evolution. The book introduces a variety of studies related to horizontal gene transfer, gene silencing, plasmids, phages, transposons, and the emergence of microbes that degrade man-made xenobiotics and have antimicrobial resistance. Written by leading researchers in DNA traffic, the book is a valuable guide to horizontal transfer for both young scientists and experts in the field
    Pages: VII, 278 p. 39 illus., 25 illus. in color. : online resource.
    ISBN: 9789811334115
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  • 35
    Keywords: Mathematics ; Mathematics Education ; History of Mathematical Sciences ; International and Comparative Education ; Springer eBooks
    Description / Table of Contents: Chapter 1. European Didactic Traditions in Mathematics: Introduction and Overview -- Chapter 2. The French Didactic Tradition in Mathematics -- Chapter 3. Didactics of mathematics in the Netherlands -- Chapter 4. The Italian Didactic Tradition.-Chapter 5. The German Speaking Didactic Tradition -- Chapter 6. Didactics of Mathematics as a Research Field in Scandinavia -- Chapter 7. Czech and Slovak Research in Didactics of Mathematics Tradition and a Glance at Present State
    Abstract: This open access book discusses several didactic traditions in mathematics education in countries across Europe, including France, the Netherlands, Italy, Germany, the Czech and Slovakian Republics, and the Scandinavian states. It shows that while they all share common features both in the practice of learning and teaching at school and in research and development, they each have special features due to specific historical and cultural developments. The book also presents interesting historical facts about these didactic traditions, the theories and examples developed in these countries
    Pages: V, 212 p. 28 illus., 10 illus. in color. : online resource.
    ISBN: 9783030055141
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  • 36
    Keywords: Biochemistry ; Neurosciences ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease
    Abstract: This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects
    Pages: XII, 289 p. 66 illus., 55 illus. in color. : online resource.
    ISBN: 9781493991242
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  • 37
    Keywords: Biomedical Engineering ; Microbiology ; Biomedical Engineering/Biotechnology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Pathway and Gene Discovery from Natural Hosts and Organisms -- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity -- Antisense RNA Elements for Downregulating Expression -- Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering -- Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs -- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae -- Assembly of Complex Pathways using Type IIs Restriction Enzymes -- Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly -- Checks and Balances with Use of the Keio Collection for Phenotype Testing -- Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803 -- Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica -- Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization -- Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization -- A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models -- 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms -- A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast
    Abstract: This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications
    Pages: X, 252 p. 46 illus., 39 illus. in color. : online resource.
    ISBN: 9781493991426
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  • 38
    Keywords: Medicine ; Cell Culture ; Nephrology ; Molecular Medicine ; Cell Culture ; Nephrology ; Springer eBooks
    Description / Table of Contents: Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development -- Mouse Ex Vivo Kidney Culture Methods -- Serum-Free Organ Culture of the Embryonic Mouse Ureter -- Experimental Tubulogenesis Induction Model in the Mouse -- Dissociation of Tissues for Single Cell Analysis -- Long Term Culture of Nephron Progenitor Cells Ex Vivo -- 3D Cell Culture Models of Epithelial Tissues -- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells -- Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells -- Protocol to Generate Ureteric Bud Structures from Human iPS Cells -- Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture -- Virus as Renal Functional Genetic Tools -- Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines -- Time-Lapse Technologies and 4D Imaging of Kidney Development -- Decellularizing and Recellularizing Adult Mouse Kidneys -- Optical Projection Tomography Imaging to Study Kidney Organogenesis -- Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model -- Analysis of the Teashirt Target Genes in Ureteric Bud Development -- Methods of Computational Analysis in Kidney Development
    Abstract: This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 248 p. 57 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990214
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  • 39
    Keywords: Human Physiology ; Biochemistry ; Human Physiology ; Medical Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Part 1 Metabolic Disorders. Citrin deficiency” -- Homocystinuria No Acquaintance -- Phenylketonuria -- Urea cycle disorders (such as defects of OTCD)” -- Wilson disease -- Diabetes Mellitus type I -- Lipid storage disorder, namely Niemann-Pick C1 disease -- Gaucher disease -- Fabry disease -- Mitochondrial disease (such as defects of MELAS) -- Heme oxygenase deficiency -- Collagen metabolism (such as OI) -- Organic acid metabolism disorders -- Part 2 Genetic Disorders. Glucose 6-Phosphate Dehydrogenase Deficiency -- Familial Hypercholesterolemia -- Fukuyama muscular dystrophy -- Hemoglobin -- Huntington disease -- Anti-coagulant deficiency -- Auto-inflammatory disorders -- Cancer -- Marfan syndrome -- Human immunodeficiency virus infection -- Hepatitis virus C -- Cytomegalovirus infection (especially congenital infection) -- Addiction
    Abstract: This textbook uses a case-study approach to present the core principles of biochemistry and molecular biology in the context of human disease to students who will be involved in patient care. The 29 clinical cases have been carefully selected to cover key scientific concepts and some common, and other not so common, diseases. While the principal focus is on topics relating to metabolic disease, further subjects such as connective tissue disorders, neurological disorders, auto-inflammatory disorders, infective diseases, and cancer are also addressed. Each chapter provides a specific patient report that includes the natural history, pertinent clinical laboratory data, physical findings, subsequent diagnosis, and therapy. This is followed by a comprehensive discussion of the normal biochemical processes and reactions pertaining to the case, along with the pathophysiological mechanisms of the disease. Graphical diagrams are provided in each chapter for ease of comprehension
    Pages: XI, 349 p. 167 illus., 113 illus. in color. : online resource.
    ISBN: 9789811329777
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  • 40
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism
    Abstract: This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories
    Pages: XI, 495 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990276
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  • 41
    Keywords: Pharmaceutical technology ; Pharmacy ; Nanochemistry ; Pharmaceutical Sciences/Technology ; Pharmacy ; Nanochemistry ; Springer eBooks
    Description / Table of Contents: Understanding the Surface Characteristics of Nanoparticles -- Surface Modification of Nanoparticles for Targeted Drug Delivery -- Surface-Modified PLGA Nanoparticles for Targeted Drug Delivery to Neurons -- Nanoparticles for Site Specific Drug Delivery -- Stealth Properties of Nanoparticles against Cancer: Surface Modification of NPs for Passive Targeting to Human Cancer Tissue in Zebra Fish -- Passive targeting of nanoparticles to cancer -- Surface Modification of Magnetic Nanoparticles in Biomedicine -- A novel strategy for the surface modification of super paramagnetic (Fe3O4) iron oxide nanoparticle for lung cancer imaging -- Biodegradable nanoparticles for drug delivery and targeting -- Functionalized antibacterial nanoparticles in controlling biofilm and intracellular infections -- Surface modifications of liposomes for drug targeting -- Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system -- Surface Modification of Metallic Nano particles -- Successful Delivery of Zidovudine-loaded Docosanol Nanostructured Lipid Carriers (Docosanol NLCs) into rat brain -- Brain targeted drug delivery with surface modified nanoparticles -- Surface Modification of Resorcinarene Based Self-Assembled Solid Lipid Nanoparticles for Drug Targeting -- Targeted delivery of surface modified nanoparticles:Modulation of inflammation for acute lung injury -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface Modification of Gold Nanoparticles for Targeted Drug Delivery -- Surface modification and bioconjugation of nanoparticles for MRI technology -- Surface Modified Lanthanide Nanomaterials for Drug Delivery -- Engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers -- Application of Nanoparticles in treating Periodontitis: Preclinical and Clinical overview -- Surface Modification of Nanoparticles for Ocular Drug Delivery
    Abstract: This unique book discusses various new techniques developed to enhance the application of nanoparticulate drug delivery systems using surface modification of nanoparticles. The understanding of the surface characteristics of nanoparticles is growing significantly with the advent of new analytical techniques. Polymer chemistry is contributing to the development of many new versatile polymers which have abilities to accommodate many different, very reactive chemical groups, and can be used as a diagnostic tool, for better targeting, for more effective therapeutic results as well as for reducing the toxic and side effects of the drugs. Surface modification of such polymeric nanoparticles has been found by many scientists to enhance the application of nano-particles and also allows the nanoparticles to carry specific drug molecule and disease /tumor specific antibodies which refine and improve drug delivery. Surface Modification of Nanoparticles for Targeted Drug Delivery is a collection of essential information on various applications of surface modification of nano-particles and their disease specific applications for therapeutic purposes
    Pages: XVIII, 499 p. 142 illus., 110 illus. in color. : online resource.
    ISBN: 9783030061159
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  • 42
    Keywords: Medical parasitology ; Emerging infectious diseases ; Microbial genetics ; Microbial genomics ; Parasitology ; Infectious Diseases ; Microbial Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome -- Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development -- Transcriptome Studies in Trypanosoma cruzi using RNAseq -- An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene -- Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi -- Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes -- Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi -- Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae -- Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi -- Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase -- Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions -- Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues -- In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters -- Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models -- MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice -- Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR -- Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR -- Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase -- Analytics of Functional Autoantibodies in Patients with Chagas’ Disease -- Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease -- Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma -- Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease -- Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients -- Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients -- Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease -- CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease -- Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease -- Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts
    Abstract: This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information
    Pages: XVII, 398 p. 60 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991488
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  • 43
    Keywords: Medicine ; Dentistry ; Molecular Medicine ; Dentistry ; Springer eBooks
    Description / Table of Contents: Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors -- Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line -- Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells -- Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors -- Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis -- Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions -- Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering -- Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry -- Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering -- In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy -- A Mouse Model to Study Reparative Dentinogenesis -- Multiwalled Carbon Nanotubes for Dental Applications -- Peptide-mediated Biomimetic Regrowth of Human Enamel in situ -- Bioengineering Tooth Bud Constructs using GelMA Hydrogel -- Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes -- In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes -- Methods for in situ Proteins Visualization in Dental Mineralized Tissues -- In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection -- Immunofluorescence Procedure for Developing Enamel Tissues -- Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos -- Isolation of SIBLING Proteins from Bone and Dentin Matrix -- Immunohistochemical Co-localization of Amelogenin and Ameloblastin in Developing Enamel Matrix -- The Expression and Purification of Recombinant Mouse Ameloblastin in e-coli -- Protocols for Studying Formation and Mineralization of Dental Tissues in vivo -- Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth -- Purification of Developing Enamel Matrix Proteins using Preparative SDS PAGE -- Using ImageJ (FIJI) to Analyse and Present X Ray CT Images of Enamel -- Scanning Electron Microscopy (SEM) Methods for Dental Enamel -- Microcomputed Tomography Imaging in Odontogenesis Studies -- Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues -- Rodent Dental Fluorosis Model--Extraction of Enamel Organ from Rat Incisors -- Three Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-beam Computed Tomography (CBCT) -- Protocols to Study Dental Caries In Vitro: Microbial Caries Models -- In vitro Caries Models for the Assessment of Novel Restorative Materials -- Protocols to Study Dental Caries In Vitro: pH Cycling Models -- In Vivo Rodent Models for Studying Dental Caries and Pulp Disease -- Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement -- Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues -- Protocols, Methods, and Tools for Genome-wide Association Studies (GWAS) of Dental Traits -- Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition -- The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Oral Metagenomics, Metatranscriptomics and Metabolomics Studies of the Oral Microbiome -- Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarkers Screening
    Abstract: This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth
    Pages: XVIII, 565 p. 152 illus., 135 illus. in color. : online resource.
    ISBN: 9781493990122
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  • 44
    Keywords: Human Genetics ; Nanotechnology ; Biotechnology ; Toxicology ; Human Genetics ; Nanotechnology ; Biotechnology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection -- Synthesis of Bioreducible Polycations with Controlled Topologies -- Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery -- Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates -- Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems -- Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids -- Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery -- Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation -- Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol -- Layer-By-Layer Assembled Nanoparticles for siRNA Delivery -- Layer-By-Layer Film Engineering for Sequential Gene Delivery -- Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles -- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films -- Lyophilization of Synthetic Gene Carriers -- Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery -- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles -- Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery -- Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity -- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity -- Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS) -- Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining -- Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery -- Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo -- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery -- Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation -- Lipopeptide Delivery of siRNA to the Central Nervous System
    Abstract: This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself
    Pages: XV, 406 p. 107 illus., 71 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990924
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  • 45
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 46
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK! -- Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis -- Identification of Long Non-Coding RNAs in the Developing Endosperm of Maize -- RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize -- Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues -- Medium Throughput RNA In Situ Hybridization of Serial Sections from Paraffin-Embedded Tissue Microarrays -- Purification and Functional Analysis of Plant Long Non-Coding RNAs (lncRNA) -- The Involvement of Long Non-Coding RNAs in Response to Plant Stress -- Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance -- Discovery, Identification, and Functional Characterization of Plant Long Intergenic Non-Coding RNAs after Virus Infection -- Bioinformatics Approaches to Studying Plant Long Non-Coding RNAs (lncRNAs): Identification and Functional Interpretation of lncRNAs from RNA-seq Data Sets -- Identification of Novel lincRNA and Co-Expression Network Analysis using RNA Sequencing Data in Plants -- An Easy-to-Follow Pipeline for Long Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria vesca -- Reference-Based Identification of Long Non-Coding RNAs in Plants with Strand-Specific RNA-Sequencing Data -- NAMS: Non-Coding Assessment in Magnoliophyta Species -- De novo Plant Transcriptome Assembly and Annotation using Illumina RNA-seq Reads -- Identification of Long Non-Coding RNA-Protein Interactions through In Vitro RNA Pull-Down Assay with Plant Nuclear Extracts -- Analysis of Interaction between Long Non-Coding RNAs and Protein by RNA-Immunoprecipitation in Arabidopsis -- Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants -- In Vivo Genome-Wide RNA Structure Probing with Structure-seq2 -- Using Protein Interaction Profile Sequencing (PIP-seq) to Identify RNA Secondary Structure and RNA-Protein Interaction Sites of Long Non-Coding RNAs in Plants -- Computationally Characterizing Protein Bound Long Non-Coding RNAs and their Secondary Structure using Protein Interaction Profile Sequencing (PIP-seq) in Plants -- Stalking Structure in Plant Long Non-Coding RNAs -- Transcriptome-Wide Mapping 5-methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant -- A Walkthrough to the Use of GreeNC: The Plant lncRNA Database -- CANTATAdb 2.0: Expanding the Collection of Plant Long Non-Coding RNAs -- Experimentally Validated Plant lncRNAs in EVLncRNAs Database -- In Situ Hi-C for Plants: An Improved Method to Detect Long-Range Chromatin Interactions
    Abstract: This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology
    Pages: XVII, 480 p. 78 illus., 67 illus. in color. : online resource.
    ISBN: 9781493990450
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  • 47
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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  • 48
    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 49
    New York, NY : Springer
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: Nonsurgical Hammertoe Treatment Options -- PIPJ Arthroplasty without Fixation -- PIPJ Interpositional Arthroplasty -- PIPJ Arthroplasty with Temporary Kirchner Wire Fixation -- PIPJ Arthrodesis, Peg and Hole -- PIPJ Arthrodesis via Buried Kirchner Wire -- PIPJ Arthrodesis with a 1 Component Metallic Implant -- PIPJ Arthrodesis with a 2 Component Metallic Implant -- PIPJ Arthrodesis with a Thermal Activated Metallic Implant -- PIPJ Arthrodesis with an Allograft Implant -- PIPJ Arthrodesis with a Bioabsorbable Implant -- Flexor Tendon Transfer -- Tenotomies -- MTP Joint: Soft Tissue Balancing (skin plasties and lengthenings, MTPJ release, extensor tendon lengthening, capsulotomy, capsulorraphy) -- MTP Joint: Plantar Plate Reconstruction via a Dorsal Approach -- MTP Joint: Plantar Plate Repair via a Plantar Approach -- Lesser Metatarsal Osteotomies: uniplanar, biplanar wedge osteotomies -- MTP Joint: Transverse Plane Correction via Collateral Ligament Repair -- MTP Joint Transverse Plane Correction via Metatarsal Osteotomy -- Crossover Toe Correction -- Congenital Overlapping Fifth Toe -- Flail Hammertoe Correction
    Abstract: Comprised exclusively of clinical cases demonstrating the various management strategies for hammertoes, this concise, practical casebook will provide foot and ankle surgeons with the best real-world strategies to properly treat these frequent deformities of the lower extremity and their complications. Beginning with a review of the relevant anatomy and biomechanics of the toe, each subsequent chapter includes case material with a unique clinical presentation, followed by a description of the diagnosis, assessment and management techniques used to treat it, as well as the case outcome, literature review and clinical pearls and pitfalls. Chapters included illustrate different surgical fixation techniques, including PIPJ arthroplasty and arthrodesis, tendon transfers and tenotomies, and osteotomies, as well as management strategies for complications. Pragmatic and reader-friendly, Hammertoes: A Case-Based Approach is an excellent resource for foot and ankle surgeons treating this common but frustrating condition
    Pages: XIII, 419 p. 511 illus., 427 illus. in color. : online resource.
    ISBN: 9783319165523
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  • 50
    Keywords: Biochemistry ; Bioinformatics ; Protein Structure ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 55 Years of the Rossmann Fold -- Advances in Protein Super-Secondary Structure Prediction and Application to Protein Structure Prediction -- Automated Family-Wide Annotation of Secondary Structure Elements -- Computational Prediction of Secondary and Supersecondary Structures from Protein Sequences -- StackSSSPred: A Stacking Based Prediction of Supersecondary Structure from Sequence -- Information-Theoretic Inference of an Optimal Dictionary of Protein Supersecondary Structures -- Secondary and Supersecondary Structure of Proteins as Coupling between Local and Local and Backbone-Electrostatic Interactions: A View through Cluster-Cumulant Scope -- Learning Organizations of Protein Energy Landscapes: An Application on Decoy Selection in Template-Free Protein Structure Prediction -- Exploring Protein Supersecondary Structure through Changes in Protein Folding, Stability, and Flexibility -- Protodomains: Symmetry Related Supersecondary Structures in Proteins and Self-Complementarity -- bab Super Secondary Motifs: Sequence, Structural Overview and Pursuit of Potential Autonomously Folding bab Sequences from (b/a)8/TIM Barrels -- Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for their Prediction and Characterization -- Stable Sub-Structures in Proteins and How to Find Them using Single-Molecule Force Spectroscopy -- Supersecondary Structures and Fragment Libraries -- Molecular Dynamics Simulations of Conformational Conversions in Transformer Proteins -- Sequence Pattern for Supersecondary Structure of Sandwich-Like Proteins -- Homology Searches using Supersecondary Structure Code -- Beyond Supersecondary Structure: Physics-Based Sequence Alignment -- Secondary and Supersecondary Structure of Proteins in Light of the Structure of Hydrophobic Cores -- Hands On: Using Tryptophan Fluorescence Spectroscopy to Study Protein Structure -- Structural Characterization of Membrane Protein Dimers
    Abstract: This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation
    Pages: XVIII, 438 p. 119 illus., 103 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991617
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  • 51
    New York, NY : Springer
    Keywords: Bacteriology ; Microbiology ; Biochemistry ; Bacteriology ; Applied Microbiology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Refined Systematics of the Genus Rhodococcus Based on Whole Genome Analyses -- Genomics of Rhodococcus -- Central Metabolism of Species of the Genus Rhodococcus -- Oligotrophic Growth of Rhodococcus -- Adaptation of Rhodococcus to Organic Solvents -- Degradation of Alkanes in Rhodococcus -- Biodegradation of Nitriles by Rhodococcus -- The Desulfurization Pathway in Rhodococcus -- Bioremediation of Contaminated Environments Using Rhodococcus -- Production of Trehalolipid Biosurfactants by Rhodococcus -- Biology of Triacylglycerol Accumulation by Rhodococcus -- Interaction of Rhodococcus with Metals and Biotechnological Applications -- Plant-Associated Rhodococcus Species, for Better and for Worse
    Abstract: Rhodococcus, a metabolically versatile actinobacterium which is frequently found in the environment, has gained increasing interest due to its potential biotechnological applications. This Microbiology Monographs volume provides a thorough review of the various aspects of the biochemistry, physiology and genetics of the Genus Rhodococcus. Following an overview of its taxonomy, chapters cover the structural aspects of rhodococcal cellular envelope, genomes and plasmids, metabolic and catabolic pathways, such as those of aromatic compounds, steroids and nitriles, and desulfurization pathways, as well as adaption to organic solvents. Further reviews discuss applications of Rhodococcus in the bioremediation of contaminated environments, in triacylglycerols accumulation, and in phytopathogenic strategies, as well as the potential of biosurfactants. In addition, properties of these bacteria to thrive and survive in oligotrophic environments are also discussed. A final chapter describes the sole pathogenic Rhodococcus member, R. equi. This book is intended for undergraduate, graduate and postdoc researchers and students interested in a wide range of areas of knowledge related to the bacteria of the genus Rhodococcus. In addition, this work will be useful for professionals who work in companies related to biotechnology and commercial use of microorganisms
    Pages: XI, 386 p. 52 illus., 27 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030114619
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  • 52
    New York, NY : Springer
    Keywords: Surgery ; Head / surgery ; Pneumology ; Oral and Maxillofacial Surgery ; Head and Neck Surgery ; Pneumology/Respiratory System ; Springer eBooks
    Description / Table of Contents: Medical Evaluation of Patients with Obstructive Sleep Apnea (OSA) -- Interpretation and Analysis of Polysomnography -- Medical Management -- Surgical Evaluation and Airway Assessment of Patients with OSA -- Nasal Surgery -- Palatal Surgery -- Base of Tongue Surgery -- Hypoglossal Nerve Stimulation -- Genioglossus Advancement -- Maxillo-Mandibular Advancement -- Operative Airway Management and Tracheostomy -- Considerations and Management of Pediatric OSA
    Abstract: This book provides detailed, specific information regarding the non-surgical and surgical treatment modalities currently employed for the management of obstructive sleep apnea (OSA) with the aim of enabling practitioners to achieve optimal outcomes in individual patients. The book opens by offering clear guidance on the medical and surgical evaluation of patients with OSA. Coverage of medical management options includes positive airway pressure therapy, positional therapy, the use of mandibular repositioning devices, along with other treatment modalities. Surgical interventions are then exhaustively described in a series of chapters that document the approaches to specific sites of obstruction. Information is included on operative airway management, and the concluding chapter addresses the care of pediatric patients. Modern Management of Obstructive Sleep Apnea will be a valuable asset for oral and maxillofacial surgeons, otolaryngologists, and sleep medicine physicians
    Pages: V, 129 p. 54 illus., 49 illus. in color. : online resource.
    ISBN: 9783030114435
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