Springer Online Journal Archives 1860-2000
Process Engineering, Biotechnology, Nutrition Technology
Summary The effects of residual enzyme activity, stepwise addition of lipase at different reaction times, and enzyme quantity in direct polyesterification of sebacic acid and 1,4-butanediol catalyzed by a lipase from Rhizomucor miehei were investigated. Although the lipase activity dropped sharply in the beginning period of the reaction, the molar mass of the polyester increased rapidly, up to 39,000 g mol−1 in 72 h. The residual lipase activity (hydrolytic) was only 14 %. Stepwise addition of lipase did not improve polyester synthesis. Highest mass average molar mass of 56,000 g mol−1 was obtained with 0.125 g of lipase (28.5%, w/w) in 5 days.
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