Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1980-1984  (308,246)
Collection
Years
Year
  • 1
    facet.materialart.
    facet.materialart.
    Totowa, NJ : Humana Press
    Keywords: Molecular Biology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6029
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    facet.materialart.
    New York, NY : Elsevier
    Keywords: Biochemistry ; Enzymes
    Notes: This is a series title, single volumes see link below.
    ISSN: 1557-7988
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    facet.materialart.
    facet.materialart.
    Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory
    Associated volumes
    Keywords: Cancer cells
    Notes: Please search for single volumes in "Reihe (alphabetisch)" or "Reihe (Stichwort)"
    ISSN: 0743-2194
    Subsequent Title: Leerer Normsatztext
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Call number: QZ200Z:41
    Keywords: Oncology / Congresses
    Notes: Holdidngs of ZB: 1964-1988; Please search for single title in "Signatur" QZ200Z:41
    ISSN: 0069-4800
    Signatur Availability
    QZ200Z:41 available
    BibTip Others were also interested in ...
  • 5
    facet.materialart.
    facet.materialart.
    Rockville, Md. : U.S. Dept. of Health, Education, and Welfare, Public Health Service, Center for Disease Control, National Institute for Occupational Safety and Health ; Washington :For sale by the Supt. of Docs., U.S.G.P.O.
    Call number: QV1Z:70
    Keywords: Toxicology
    Abstract: "This compilation will provide ready reference for potential toxicity of chemicals found in the workplace, and should be useful to occupational health physicians, industrial hygienists, toxicologists, and researchers." Alphabetical arrangement by substances. Entries include such details as molecular weight, Wiswesser Line Notation, synonyms, and reference from which data about toxicity derived. Miscellaneous appendixes, including one titled Aquatic toxicity. Bibliographic references
    Pages: v.
    ISSN: 0361-2546
    Signatur Availability
    QV1Z:70 available
    BibTip Others were also interested in ...
  • 6
    facet.materialart.
    facet.materialart.
    Stuttgart : Enke
    Call number: WB700:12(2)/1/Mag ; WB700:12(2)/2/Mag ; WB700:12(2)/3/Mag
    Description / Table of Contents: 1. Abth. Die allgemeinen acuten Infectionskrankheiten.--2. Abth. Die chronischen Infections- und Intoxications-Krankheiten, parasitäre Krankheiten, infectiöse Wundkrankheiten und chronische Ernährungs-Anomalieen.--3. Abth. Die Organkrankheiten
    Pages: 3 v.
    Edition: 2. vollständig neue Bearbeitung.
    Signatur Availability
    WB700:12(2)/1/Mag departmental collection or stack – please contact the library
    WB700:12(2)/2/Mag departmental collection or stack – please contact the library
    WB700:12(2)/3/Mag departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Teaching statistics 6 (1984), S. 0 
    ISSN: 1467-9639
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mathematics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 18
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 20
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Book review in this Article RECLAIMING BIRTH: HISTORY AND HEROINES OF AMERICAN CHILDBIRTH REFORM Margot Edwards and Mary Waldorf CARE FOR THE UNCIRCUMCISED PENIS CIRCUMCISION: A PERSONAL CHOICE EPISIOTOMY AND THE SECOND STAGE OF LABOR Sheila Kitzinger and Penny Simkin FAMILY-CENTERED MATERNITY CARE IMPLEMENTATION STRATEGIES Susan McKay and Celeste R. Phillips ANALYSIS OF CURRENT ASSESSMENT STRATEGIES IN THE HEALTH CARE OF YOUNG CHILDREN AND CHILDBEARING FAMILIES Sharron Smith Humenick BIRTH RIGHTS: AN ADVOCATE'S GUIDE TO ENDING INFANT MORTALITY Francie Hornstein and Judith G. Waxman THE PREGNANT WOMAN'S COMFORT GUIDE Sherry Lynn Mims OBSTETRICAL ULTRASOUND Philippe Jeanty
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : Despite the paucity of research on men's experiences of cesarean birth, fathers' attendance at cesareans has become well-established in some areas of the U.S. In this study, interviews were conducted with 46 fathers whose wives had an uncomplicated pregnancy culminating in an unanticipated cesarean birth with a healthy neonate and no major complications for mother and child. Interviews were tape recorded, transcribed and analyzed. Of these 46 fathers, 52 per cent attended the cesarean, and 48 per cent did not, primarily because hospital policy prohibited it. Fathers' predominant emotional reaction to the decision for cesarean was relief (52%); 27 per cent described their reactions as acceptance, 10 per cent expressed moderate disappointment, and 11 per cent were strongly disappointed or angry. Most negative reactions centered not on the cesarean itself, but on policies which excluded fathers from attendance arbitrarily, and on staff behaviors which reflected disregard for the fathers'need to feel included in the birth, whether they were permitted to attend the delivery or not. Seventy per cent of these fathers expressed some displeasure at physician or nurse behaviors, expressing disappointment and resentment about being excluded from discussions leading to the decision for the cesarean, having little previous contact with the obstetrician, the nursing staff failing to provide the father with adequate information and support during and immediately after the cesarean, and being denied access to the wife and infant after the cesarean for apparently arbitrary reasons.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : Some studies link postpartum depression with the 4-day “blues,” and with severe postpartum mental illness, while other studies show differences between these or define each distinctly. Research on possible contributors to postpartum depression has moved from psychoanalytic and hormonal theories to factors in the pregnancy, birth, and postpartum periods. Interpersonal and adaptational models are presented in detail.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 25
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : About one-third of women enter their pregnancies as smokers. Attempts to help those who do not quit on their own have not been effective. As part of a 3-year study to test the hypothesis that smoking reduction during pregnancy improves the birthweight of the infant (an hypothesis which was confirmed), a smoking intervention program was developed that resulted in a twofold increase in the percentage of women who stopped smoking cigarettes during their pregnancy. There was no comparative evaluation of the different kinds of intervention activities that were used, but because they were successful in the aggregate, they are described here and a judgment made of the effectiveness of the different components. The main method of contact for the counseling was a single face-to-face session at the smoker's home, followed by frequent phone contacts. Although information was given on the risks of smoking during pregnancy, the emphasis was on how to stop through the use of behavioral skills such as developing commitment, self-monitoring, stimulus control, substitute and incompatible behaviors, and positive associations with not smoking. A series of contacts through mailed information was maintained throughout the pregnancy. The findings have implications for strengthening the intervention activities that are presently offered to pregnant smokers.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 30
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : A review and analysis of studies of the effects of ultrasound exposure on human fetuses leads to a conclusion that there is no association of exposure with birthweight, hearing loss, or major congenital anomalies. Conflicting data from studies on birthweight is probably due to methodologic problems with the studies that have found positive associations. There have been few sound studies of the effects of in utero ultrasound exposure in humans. Limited studies to date are mostly reassuring, but it is premature to say that ultrasound has been proven safe for the human fetus. Further research on the question of biological effects of ultrasound in humans is needed, particularly the question of a possible association of ultrasound with dyslexia.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 31
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 32
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 33
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 35
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Book review in this Article HAVING A SECTION IS HAVING A BABY TO HAVE AND NOT TO HOLD: Helping the Parents of Premies Cope Peter A. Gorski, MD, Director JONAH HAS A RAINBOW A Film on Premature Birth Christina Gonzalez THE BEHAVIOR OF THE PREMATURE INFANT Peter A. Gorski, MD
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 36
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 37
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : To investigate the effects of the frequency and duration of breastfeedings on nipple soreness we studied two groups of mother-infant pairs during the first 10 postpartum days. Mothers in the control group fed on a 3- to 4-hour schedule and those in the experimental group were encouraged to breastfeed as frequently as the infants desired. All mothers recorded the time and duration of each breastfeeding for the first 10 days after delivery and gave their own daily assessments of nipple discomfort. Although mothers in the experimental group nursed significantly more frequently (10 vs. 7.4 feedings/24 hours, p 〉.0001), they did not experience more nipple soreness. There was no significant correlation between the frequency or duration of feedings and the degree of nipple soreness. In both groups there was a significant decrease in nipple soreness from days 1–5 to days 6–10 in spite of a significant increase in the frequency and duration of feedings over the same period. Our results suggest that frequent feedings in early lactation do not increase the incidence of nipple discomfort.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 38
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 39
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : A survey of 567 self-selected respondents who breastfed while employed outside the home was conducted to assess the legitimacy of the assumption that breastfeeding and maternal employment combine poorly. Eighty-six per cent of the respondents identified one or more difficulties, most of which were common to all parents. Breastfeeding problems were, with few exceptions, unrelated to employment. The respondents' most significant difficulty was role overload, a result of multiple demands to which they were responding. The results suggest that specific information about breastfeeding, encouragement in setting priorities, and involving the mother's partner and others in household and child care duties may reduce the level of stress of working mothers who breastfeed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 40
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: : The Consensus Development Conference on Diagnostic Ultrasound Imaging revealed that neither the energy emitted from an ultrasound transducer, nor the energy absorbed by maternal and fetal tissues has been quantitated. For convenience, the energy is expressed in watts/square centimeter. Although in vitro and animal studies have shown various bioeffects of ultrasound, none obtained under 100 mW/cm2 has been reproducible, and none can be translated reliably to humans. Four randomized controlled trials of ultrasound imaging failed to show harmful effects, and also failed to show benefits of routine screening in terms of infant mortality or morbidity. The panel accordingly recommended that ultrasound imaging be used only with a specific indication, and listed 27 indications. They recommended that women be supplied with information about exposure time, intensity, indication, alternatives, and benefits and risks of an ultrasound scan, if they request the information. The panel also recommended further research on outputs, bioeffects, and efficacy of routine screening by ultrasound during pregnancy.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 41
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 42
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 43
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 44
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 45
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Birth 11 (1984), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Book review in this Article BREASTFEEDING: A SPECIAL LOVE Videotape series SHOULD I NURSE MY BABY? 22 min. MOTHER'S MILK—NATURE'S PERFECT FORMULA: 22 min. APPROACHING BREASTFEEDING DIFFICULTIES: Part I - 25 min. CHILDBIRTH… THE CHANGING SOUNDS
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 46
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract We present a new method for extracting a comprehensive suite of biologically significant parameters from line transect data of coral communities. In addition to the percentage coral cover (the traditionally extracted parameter), the method extracts the population density of the coral colonies, their mean diameter and associated standard deviation and, for adequate data, their size frequency distribution. The method assumes only that the coral colonies form a system of non-overlapping circles in the plane, that the diameters of the circles are random quantities with an unknown distribution function, and that the transects are placed randomly. We test the method on both theoretical and real data to show that it performs as well as, if not better than, current methods in extracting the traditional parameter as well as being able to extract the additional useful parameters indicated. Because the method makes few restrictive assumptions and seems robust when used with field data, we suggest that it has wide application wherever line transects are used for ecological survey. The method is implemented in a Fortran program available from the senior author.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 47
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Previous studies have attempted to quantify the nutritional importance of zooxanthellae to the respiratory requirements of their host, from measurements of the 24 h photosynthesis to respiration ratio. This preliminary study explores an alternative approach by trying to account for the energy fixed in photosynthesis in terms of expenditure, storage and loss. The energy budget so derived for Pocillopora eydouxi suggests that about 51% of the photosynthetically fixed energy is used in respiration, 0.9% in growth and 48% is unaccounted for and presumably lost from the colony. By partitioning the energy budget, it is found that 24% of the respiration and 11% of the energy retained in growth is attributable to the zooxanthellae. It is postulated that nutritionally, a coral is analogous to an aphid in being supplied with a high carbon diet and in being obliged to excrete the excess, probably in the form of mucus.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 48
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The reproductive biology of Goniastrea aspera at Magnetic Island was compared with that of a very similar sympatric species, G. favulus as reported by Kojis and Quinn at Heron Island. The development of gametes was similar in both species, but there was no evidence for an adolescent protandrous period of development in G. aspera such as that recorded for G. favulus at Heron Island. Other reproductive differences between the two species were found in egg size and the mode of spawning. The eggs of G. aspera are smaller and more numerous than those of G. favulus. Goniastrea aspera expelled buoyant packets of eggs and sperm, while G. favulus had sticky sinking eggs which were released separately from the sperm. The spatial pattern of the two species was examined on the reef flat at Magnetic Island to determine whether the observed differences in spawning behaviour and egg buoyancy might have an effect on egg retention and the distribution of adult colonies. The results of this comparison failed to detect any difference in the degree of aggregation of the two species. This is not the result which would be expected if sticky sinking eggs helped retain developing larvae in the vicinity of the adult. These results, together with evidence from a wide range of coelenterates and observations on the larvae of G. aspera point to post spawning larval behaviour as the most likely factor in determining where these species will settle.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 49
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Fish larvae from horizontal plankton tows along a single transect near outer ribbon reefs of the Great Barrier Reef in spring 1979 and summer 1980 had persistent distributional patterns. Larvae were identified to family and divided into young (preflexion) and old (postflexion) larvae, thus giving 28 taxa abundant enough for analysis. Non-uniform larval distributions were found for 81% of the 16 reef fish taxa with non-pelagic eggs, but for only 17% of the six reef fish taxa with pelagic eggs. Most differences in larval concentration were between the lagoonal and seaward sides of the reef. Only tripterygiid larvae had highest concentration just seaward of the reef, while larvae of 12 reef and three oceanic fish taxa occurred in highest concentrations on the lagoonal side of the reef. In five taxa of reef fishes, higher larval concentrations were found in the lagoonal backreef compared with the mid-lagoon habitat; but the reverse was not found in any taxon. Eleven taxa had indeterminate distributions, (i.e. no difference in concentration between stations). Mechanisms responsible for the distribution remain unknown, but we suggest that the view which considers fish larvae to be passively-drifting particles is unjustified without more information on larval behaviour.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 50
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The effect of used drilling muds on coral health was examined by monitoring changes in calcification rate and soluble tissue protein concentration in the coral Acropora cervicornis. Exposure to 25 ppm (v/v) of one mud for 24 h reduced calcification rate in the growing tips by as much as 62%. In recovery experiments, corals were exposed to drilling muds for 24 h; some of them were allowed to recover in clean seawater for 48 h. After the 24-hour exposure, calcification rates were significantly less than those of the controls. After a 48-hour recovery period, calcification rates returned to control levels for one mud but were still significantly below control levels for another. The results indicate that the capacity for recovery after exposure cannot be predicted from the results of experiments on exposure only. Recovery capacity must be independently verified for all studies on the effects of short-term exposure to drilling muds.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 51
    Electronic Resource
    Electronic Resource
    Springer
    Coral reefs 2 (1984), S. 226-226 
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 52
    Electronic Resource
    Electronic Resource
    Springer
    Coral reefs 3 (1984), S. 51-57 
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Monthly linear extension and calcium carbonate accretion were measured over a year in the Caribbean staghorn coral, Acropora cervicornis. X-radiographs were made of cross sections of branches to analyze radial growth. Correlations were made between parameters of skeletal growth and four environmental parameters monitored over the same sampling periods: temperature, daylight hours, sun hours, plankton abundance. The results indicate that linear extension does not change during the year with the possible exception of April. It is suggested that temperatures outside an optimal range (ca. 26°–29°C for staghorn Acroporas) might cause a decrease in linear extension, however. Specific accretion (mg. mm-1) does show significant variations through the year. Calcium carbonate accretion (mean specific accretion times mean linear extension, mg. tip-1) is most strongly correlated with number of sun hours. A comparison is made between diel patterns of extension and accretion and longer term measurements. It is suggested that the accretion process is probably most influenced by some activity influenced by light. There are no annual growth bands in X-radiographs of cross-sections of the branches of A. cervicornis. This may result from secondary infilling in the skeleton.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 53
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Starting in June 1983, 25 species of hermatypic corals, gorgonians, hydrocorals, anemones and zoanthids in the San Blas Islands, Panama, began showing signs of a loss of colour leading in some cases to a white “bleached” appearance. Histologic examination of six coral species indicated that bleaching was associated with drastic reductions in the density of zooxanthellae and with the atrophy and necrosis of the animal tissue. The severity of the bleaching varied among species and many species were unaffected. The species most extensively affected were: Agaricia spp., which became completely bleached and frequently died; Montastraea annularis which bleached and continued to survive; and Millepora spp. which bleached white but quickly regained their colouration. Shallow reefs dominated by Agaricia spp. suffered the most extensive bleaching. At one site, Pico Feo, 99% of the Agaricia (32% of the living cover) was bleached. On fore reers, which were dominated by Agaricia spp. and M. annularis, the proportion of M. annularis bleached ranged from 18 to 100% and that of Agaricia spp. from 30 to 53%. Transects at Sail Rock and House Reef were surveyed in August 1983 and January 1984. At those sites, 53% of the Agaricia cover died between August and January. The remaining living cover of Agaricia and of all other species exhibited normal colouration in January. Salinity and temperature were monitored every second day at 4 m depth between May 10 and August 28, 1983 at one of the localities. Bleaching was first observed within two weeks of a 2 °C rise in temperature which occurred in late May 1983. Temperatures remained at or above 31.5 °C for the following 3 weeks and were at or above 30 °C for an additional 4 weeks. The bleaching of corals in the San Blas was most likely due to those elevanted temperatures.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 54
    ISSN: 1432-0975
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract A benthic faunal study was carried out in the tidal area of Mactan Island (Cebu, Philippines). The area was subdivided along a transect from the beach to the reef according to benthic assemblages. The sediments are largely composed of calcareous skeletal remains of the indigenous biota and surrounding calcareous rocks. The content of protein and carbohydrates of the sediment was estimated, providing an approximation of organic matter in terms of feeding efficiency. Total number of zoobenthos, both as regards the sediment samples and as to the epifaunal communities associated with seaweeds, is rather uniformly distributed justifying the 95% confidence level (P〉0.05). Distinct differences are apparent in abundance values of individual taxa. Although the study area showed the expected distribution pattern, with dominance of Nematoda (39%) living in sediment and Harpacticoida (36–66%) dwelling on Thalassia and algae, Polychaeta reveal a dominant attraction to both these habitats. The reasons for this phenomenon are discussed in relation to the absolute lack of macrofaunal predators The zoobenthos adjust their distribution and activity to fluctuating conditions of the environment. Light is mainly suggested as stimulating diel migration activities of the benthic fauna, moving upwards from the sediment to the algae and Thalassia during daytime. In a field experiment the zoobenthos was investigated for digestion activity over a diurnal cycle. The results reveal that feeding activity of zoobenthos follows a diel cycle showing maximum activity during the morning and evening obviously influenced by changes of light.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 55
    ISSN: 1432-0983
    Keywords: Chromosome map ; Yeast ; Schizosaccharomyces pombe ; Gene conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic map of the nuclear genome of the fission yeast Schizosaccharomyces pombe has been extended by mitotic and meiotic mapping data. A total of 158 markers are now assigned to the three linkage groups known in this organism, and 118 of them have been located on the corresponding chromosome map. Chromosome II and III each consist of one linkage group. There is some indication that the two large fragments which define chromosome I are meiotically linked, but the linkage observed is significant at the P = 0.05 level only. The length of the map is at least 1,700 map units, corresponding to an average of about 8 kilobases per map unit. The latter figure is comparable to the one obtained for intragenic recombination in the sup3 gene (Hofer et al. 1979). The basic frequency of gene conversion as measured for 21 genes varies according to a distribution of Poisson (with a modal value of 0.6% conversion per meiosis and per gene), in sharp contrast with Saccharomyces cerevisiae (Fogel et al. 1980) and Ascobolus immersus (Nicolas 1979). This may reflect the rarity of gene or region-specific rec alleles in S. pombe and may be related to the homothallism of this organism.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 56
    ISSN: 1432-0983
    Keywords: Schizosaccharomyces pombe ; Purine catabolism ; Purine hydroxylase ; Allantoinase ; Allantoicase ; Uricase, Ureidoglycollase and urease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. A number of classes of mutants unable to use purines have been isolated and genetically analysed. Mutants in the urol gene lack uricase, all1 lack allantoinase, ala1 lack allantoicase whilst in ure1, ure2, ure3 and ure4 genes lack urease activity. Mutants in four hyp genes are unable to convert hypoxanthine to uric acid whilst mutation in xan1 results in impaired growth with xanthine. hyp5 strains are unable to convert both hypoxanthine and xanthine to uric acid. The mutations are recessive and none of the loci are linked to each other. The possible catalytic steps involved are discussed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 57
    ISSN: 1432-0983
    Keywords: Yeast mitochondria ; Mapping of mutations ; Mitochondrial DNA sequence ; Mitochondrial ATPase complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A genetic and molecular analysis of the oli2 region in mitochondrial DNA of Saccharomyces cerevisiae has been carried out. The oli2 gene codes for subunit 6 of the mitochondrial ATPase complex. We have isolated a series of 41 mit −, temperature sensitive and oligomycin resistant mutants containing mutations located in the oli2 region. In addition the sequence in wild type mtDNA of 2820 nucleotides covering the oli2 gene and its flanking regions has been determined. A library of petite mutants which have various deletion end points in the oli2 region has been used to generate marker rescue data that allow the assignment of each mit −, temperature sensitivity and oligomycin resistance mutation to one of seven uniquely ordered genetic groups. The following physical reference points allow the limits of the physical map positions of a number of the genetic groups to be established: DNA sequence data have been obtained for three of the petites so as to determine unambiguously their relevant deletion end points and the base substitution constituting the mtDNA sequence change in one oligomycin resistant mutant [oli2-23r] has been determined to lie at nucleotide +523 of the oli2 coding region. One group of mit − mutations (group N) are known to map within the aap1 gene that is found some 700 bases upstream of the oli2 gene, and which codes for subunit 8 of the mitochondrial ATPase complex. Mutations in the remaining six groups (A-F) map within (or very close to) the coding region of the oli2 gene. Various features of the DNA sequence in and around the oli2 gene are considered, including protein coding regions, sequence divergence in the A,T-rich spacer regions, properties of G,C-rich clusters, and potential ribosome binding sites. Consideration of the transcriptional map of this region of mtDNA suggests that an abundant 4,500 nucleotide transcript may represent the first defined example of a dicistronic messenger RNA in yeast mitochondria carrying distinct coding regions (aap1 and oli2) for two different proteins.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 58
    ISSN: 1432-0983
    Keywords: Killer-Torulopsis yeast ; Cytoduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have used cytoduction and protoplast fusion to characterise the killer trait in Torulopsis glabrata. Killer trait could not be transferred by cytoduction to a non-killer strain of T. glabrata suggesting that the determinant(s) is/are not present in the cytoplasm. Consistent with this interpretation is the observation that all fusion products, where chromosomes of both the parents are represented, are killers. Non-killer segregants could be isolated from fusion products after treatment with benlate suggesting that loss of the trait is associated with non-disjunction and loss of a chromosome.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 59
    ISSN: 1432-0983
    Keywords: Chloroplast ; Ribosomal proteins ; Spinacia oleracea ; Phosphorylation ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have characterized the ribosomal proteins from Spinacia chloroplasts using two-dimensional gel electrophoresis. The 30S and 50S subunits contain 23–25 and 36 ribosomal proteins, respectively. In contrast to prokaryotic ribosomes, chloroplast ribosomes contain at least one (and possibly two) phosphorylated ribosomal proteins. Isolated chloroplasts synthesize in the presence of (35S) labeled methionine and cysteine at least seven 30S and thirteen 50S ribosomal proteins which are assembled into (pre)ribosomes. This suggests that about one third of the chloroplast ribosomal proteins is encoded by the chloroplast DNA itself. The identity of several labeled proteins in the two-dimensional gel electrophoretic patterns which did not comigrate with stained chloroplast ribosomal proteins is discussed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 60
    ISSN: 1432-0983
    Keywords: Pichia guilliermondii ; Regulatory mutants ; Riboflavin transport ; α-Glucosidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The method for a positive selection of Pichia guilliermondii yeast mutants which constitutively synthesize riboflavin (RF) permease has been developed. A genetic analysis revealed two regulatory genes of negative action (RFP80, RFP81) and one gene of positive action (RFP82); mutations in these loci determined the ability to synthesize RF permease in the medium without an inducer (α-glucosides). The constitutive mutants with cold-sensitive products of RFP80 and RFP81 genes were isolated. Interafelic complementation within RFP80 locus as well as restoration of the wild (inducible) phenotype in some hybrids between recessive rfp80 mutants and dominant RFP82 c mutants were observed. These data suggest a protein structure of products of identified regulatory loci and a direct interaction of the products of RFP80 and RFP82 genes. A meiotic segregants unable to synthesize RF permease in the inducer-containing media (genotype rfp82) were isolated by means of intragenic recombination in RFP82 locus. Epistasis-hypostasis test showed that gene RFP82 acted after gene RFP80. RFP80, RFP81 and RFP82 loci are involved in regulation of biosynthesis of both RF permease and α-glucosidase. The model for action of RFP80 and RFP82 gene products in the expression of RF permease and α-glucosidase structural genes of P. gulliermondii is presented.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 61
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial DNA ; Antibiotic resistance mutations ; Suppressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Strains that are genotypically sensitive to chloramphenicol and also contain one of the nuclear suppressors of mitochondrial chloramphenicol resistance (Waxman et al. 1979) were constructed. A manganese mutagenesis on such a strain produced chloramphenicol resistant mutants, most of which resulted from mutations in nuclear genes. These mutants may be either dominant or recessive, and they probably do not code for membrane proteins. The few mitochondrial mutants fall into several classes, but all result from mutations in the 21S rRNA gene. The suppressor allele effectively prevents the appearance of the most common group of mitochondrial mutants (those that map at cap1), and thereby enhances the selection of novel mutants in the region.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 62
    ISSN: 1432-0983
    Keywords: Senescence ; Podospora ; Mitochondrial DNA ; Ethidium Bromide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of ethidium bromide (EB) which is known to be able to “rejuvenate” senescent mycelia in Podospora anserina, has been investigated at the level of the mitochondrial DNA (mtDNA) by restriction analysis and molecular hybridization. While senescent mycelia display a very low growth ability and gross mtDNA modifications (tandem amplification of short sequences and disorganization of the mitochondrial chromosome: deletion of large sequences), the rejuvenated mycelia display a normal life span and contain a mtDNA in all respects identical to that of wild type mycelium (neither circular molecules nor amplified fragments could be detected). These results demonstrate a strict correlation between the senescent state and the presence of amplified mtDNA and suggest that EB rejuvenation could proceed by an efficient selection of intact mitochondrial chromosomes still present in senescent cultures.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 63
    ISSN: 1432-0983
    Keywords: Translation suppression ; Regulatory genes ; Aspergillus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The areA gene of Aspergillus nidulans is a one of the better studied eukaryotic wide domain regulatory genes, necessary for the expression of most structural genes involved in the utilization of a wide variety of nitrogen sources (Arst and Cove 1973; Arst 1983). Here we report the isolation and properties of areA alleles suppressible by translational suppressors (Roberts et al. 1979). Thus we show formally that the areA gene specifies a protein rather than an RNA product and we show that it is possible to generate by external suppression areA gene products with modified properties.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 64
    ISSN: 1432-0983
    Keywords: Petunia hybrida chloroplast DNA ; E. coli minicells ; rbcL gene ; Nucleotide sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Petunia hybrida rbcL gene was identified and located on the physical map within the Sall S9 fragment of the Petunia hybrida cpDNA by heterologous hybridization with the cloned rbcL gene of spinach (pSoc3BE148). In E. coli minicells harbouring the S9 fragment inserted into pBR322, the rbcL polypeptide is synthesized as was shown by molecular weight determination, immunoprecipitation and proteolytic digestion. However, the size of the rbcL polypeptide synthesized in minicells appeared to be dependent on the orientation of the S9 fragment in pBR322. In minicells harbouring the S9 fragment inserted into pBR322 in the clockwise orientation the molecular weight of the rbcL polypeptide is approximately 53 kD, whereas in minicells harbouring the S9 fragment in the opposite orientation, the rbcL polypeptide synthesized has a molecular weight of 52 kD. The difference in molecular weight of the two rbcL polypeptides is the result of transcription and translation into the flanking pBR322 sequences. This is due to the absence of the terminal part (6 codons), including the translation stop codon, of the rbcL gene on the cloned S9 fragment as was determined by nucleotide sequencing. The observed expression of the cloned part of the rbcL gene of Petunia hybrida indicates that the E. coli minicell system can be used as a suitable and convenient system for the identification and physical mapping of chloroplast genes. Comparison of the sequence of the untranslated 3′-end of the rbcL gene of Petunia hybrida with that of Nicotiana tabacum revealed a striking similarity of the region in which stem and loop structures can be formed that are most likely involved in termination of transcription of the rbcL gene. This region appears to be highly conserved in the rbcL genes of P. hybrida, N. tabacum, S. oleracea and Z. mays.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 65
    ISSN: 1432-0983
    Keywords: Regulation ; Alcohol dehydrogenases ; Aspergillus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Aspergillus nidulans there are two alcohol dehydrogenases. In the presence of ethanol, alcohol dehydrogenase I (AHH I) is induced and alcohol dehydrogenase II (ADH II) is repressed. ADH I and ADH II have molecular weights of 39,000 and 36,000 respectively. At least ADH I is under the control of alcR, a transacting regulatory gene that is adjacent to alcA (the structural gene for ADH I, Pateman et al. 1983). Mutations in the alcR regulatory gene result in non inducibility of ADH I specific mRNA. Extreme alcA and alcR mutations result in derepressed levels of ADH II, and it is not clear whether alcR controls ADH II directly or through its control of ADH I synthesis. Both enzymes are subject to carbon catabolite repression. Induction of ADH I and ADH II operates at the level of synthesis or processing of mRNA.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 66
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 8 (1984), S. 261-264 
    ISSN: 1432-0983
    Keywords: Phycomyces ; Photocarotenogenesis ; Quantitative complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of Phycomyces defective in carotene regulation were studied in relation with the photo carotenogenic response. Quantitative complementation analyses revealed that carC, carA and carS mutations are recessive and complement for this photoresponse in carC * carA and carC * carS heterokaryons. A scheme of the regulation of carotenogenesis by light is proposed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 67
    ISSN: 1432-0983
    Keywords: DHF Reductase ; Cloning ; Trimethoprim ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The dihydrofolate reductase gene of Saccharomyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the restriction map of which appears different from those reported for the E. coli and the mouse dihydrofolate reductase genes. The enzyme encoded by the chimeric plasmid was established as yeast dihydrofolate reductase by its sensitivity to antifolates in vivo through growth studies and in vitro by enzyme assay. Since, the expression of this gene occurs independent of its orientation within the chimeric plasmid, the 1.76 kb fragment may contain functional regulatory sequences in addition to the structural sequences for yeast dihydrofolate reductase.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 68
    ISSN: 1432-0983
    Keywords: β-isopropylmalate dehydrogenase ; Leucine B ; Leucine 2 ; Cephalosporium acremonium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Cephalosoporium acremonium gene library has been constructed using cosmid vector pYcl. After transformation of E. coli leuB strain JA221 with cosmids isolated from this gene library a cosmid (pCeleuB43) was isolated which complements the leucine auxotrophy of JA221. The same cosmid is also able to complement a leu2 strain of S. cerevisiae. The leuB and leu2 genes both code for β-isopropylmalate dehydrogenase. The complementation of a leuB E. coli strain and a leu2 S. cerevisiae strain strongly suggests that the analogous gene from C. acremonium has been isolated.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 69
    ISSN: 1432-0983
    Keywords: mRNA stability ; Nonsense mutant ; Eukaryotic transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic map of 27 mutants of the URA1 yeast gene has been established by meiotic recombination and 16 nonsense mutations characterized. The half life of URA1 mRNA was determined by two independent methods in the wild-type and in two ochre mutants localized at each extremity of the genetic map. A halflife of 15 min was found for the wild-type and for one of the ochre mutants. This half-life was radically reduced in the other ochre mutant whereas the instantaneous rate of its mRNA synthesis remained constant. After subcloning various endonucleolytic fragments the coding sequence of the URA1 gene was restricted to a 1.65 kb fragment within a 5.7 kb yeast DNA segment. Direct visualization of the URA1 mRNA by Northern hybridization of denatured RNA with a URA1 specific DNA probe revealed a length of 1.5 kb.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 70
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Pearl millet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast DNA from pearl millet (Pennisetum americanum) was used to construct recombinant plasmids. These plasmids contained 97 kilobase pairs of unique DNA sequences. The chloroplast DNA fragments in these plasmids were mapped with the restriction endonucleases SalI, SphI, XhoI, BglI and HpaI. The technique of overlapping hybridization or chromosome walking was used to orient these DNA fragments on a restriction endonuclease map of the chloroplast genome. The size of the chloroplast DNA from pearl millet was estimated in this fashion to be 127–138 kilobase pairs. Twenty one kilobase pairs of the cloned DNA fragments were represented twice on the genome as inverted repeats. Thus, the recombinant plasmids which were isolated contained approximately 86–93% of the nucleotide sequences in the chloroplast genome of pearl millet. Previously characterized cloned chloroplast DNA sequences from other plants were used as hybridization probes to locate the genes for the large subunit of ribulose 1,5-bisphosphate carboxylase, the β-coupling factor of ATPase and the 32 kilodalton polypeptide of photo system 11 on the restriction endonuclease map of the pearl millet chloroplast genome.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 71
    ISSN: 1432-0983
    Keywords: Petunia hybrida chloroplast DNA ; E. coli minicells ; ATPsynthase ; Gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Escherichia coli minicells harbouring the cloned restriction fragment Sall S9 from P. hybrida chloroplast DNA synthesize the beta and epsilon polypeptide subunits of the CF1 component of the chloroplast ATPsynthase complex. The polypeptides were identified by molecular weight determination and immunoprecipitation. The position of the atpB and the atpE gene, encoding respectively the beta and epsilon subunit, on the Sall S9 fragment was determined in more detail by studying polypeptide synthesis directed by subclones of the S9 fragment in E. coli minicells. The atpB and atpE genes are located close to the rbcL gene, the distance between the rbcL gene and atpB gene being approximately 770 bp. Analysis of the expression of subclones of the S9 fragment in E. coli minicells also revealed that the atpE gene can be transcribed and translated independently of the expression of the atpB gene. The location of the genes coding for the alpha subunit (atpA gene) and the proteolipid subunit III of CF0 (atpH) of the ATPsynthase complex on the physical map of P. hybrida cpDNA was determined by hybridization of restriction enzyme digests of petunia cpDNA with cloned cpDNA fragments from Spirodela and wheat, containing internal parts of respectively the atpA and the atpH gene. The two genes map close together within a region of 5.2 kbp on the physical map of P. hybrida cpDNA. The distance between the atpA gene and the atpB and atpE genes is approximately 42 kbp.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 72
    ISSN: 1432-0983
    Keywords: Podospora anserina ; mt plasmid ; DNA sequence ; Mobile intron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the ascomycete Podospora anserina strain ageing (senescence) is caused by a mitochondrial plasmid. In juvenile mycelia it is an integral part of the mtDNA and becomes liberated during ageing. The nucleotide sequence of this plasmid and of its flanking regions was determined. It consists of 2,539 by and contains an un identified reading frame (URF) originating in the adjacent mtDNA upstream of excision point 1. Within the URF a putative 48 by autonomously replicating sequence (ars) was identified. At both excision sites of the plasmid there are two short nonidentical interrupted palindromes and a few base pairs apart from these palindromes, both upstream and downstream, two short inverted repeats are localised. The experimental data make it evident that the mt plasmid is an intron of the cytochrome c oxidase gene (subunit I) which may be excised at the DNA level and thus become the mobile infective agent causing senescence. The concept of this mobile intron and current hypotheses concerning the relationship between introns and transposons are stressed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 73
    ISSN: 1432-0983
    Keywords: Phytophthora infestans ; mtDNA ; Phatotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA (mtDNA) of Phytophthora infestans has been isolated and preliminarily characterized. It has a low GC content of about 22.4% which is distinctly different from that of nuclear DNA (51 %). This property has been used to separate both DNA species in the presence of 4′,6-diamidine-2-phenylindole (DAPI) in CsCl density gradients. The use of cetyl triammonium bromide (CTAB) for extraction of DNA significantly reduced its degradation. The base distribution of the mtDNA shows a limited intramolecular heterogeneity. The molecule contains 36.2 ± 0.3 kb as revealed by endonuclease digestion and seems to be circular as shown by restriction mapping. No differences were found in restriction patterns between mtDNAs from various pathotypes.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 74
    ISSN: 1432-0983
    Keywords: Sugar beets ; Cytoplasmic male sterility ; Mitochondrial and chloroplast DNAs ; Restriction endonucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) and chloroplast (ct) DNAs were prepared from normal (N) and male sterile (S) cytoplasmic lines of sugar beet. The DNAs were cleaved with BamHI, EcoRI, HindIII and SalI enzymes, and the resultant fragments were fractionated by agarose gel electrophoresis. The results showed that N and S cytoplasms contained distinct mtDNA. Although most of the DNA fragments were common to these two cytoplasms, each cytoplasm was readily characterized by bands specific to that cytoplasm. In addition, these distinctive cleavage patterns were invariant in different nuclear backgrounds. In contrast to the marked variation in mtDNA, restriction fragment analyses of ctDNA demonstrated little difference between both cytoplasms. Only HindIII digestion showed one band missing in the S genome. The data presented here provides circumstantial evidence for mitochondrial involvement in the inheritance of cytoplasmic male sterility in sugar beet.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 75
    ISSN: 1432-0983
    Keywords: Yeast mitochondrial DNA ; Maturase-like sequences ; End-of-Messenger signal ; Frameshifts ; GC clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence (“RF2”) has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major family of intronic open reading frames. The RF2 open reading frame is not continuous, however, for it is interrupted by two GC clusters, both of which ultimately result in a −1 frameshift. Comparison with RF1 reveals a third insertion. This is centered on an oligo nucleotide, AATAATATTCTTA, which is found (sometimes in a slightly modified form) downstream of ten proven or suspected protein coding genes, including RF1 and RF2, and is known to terminate the apocytochrome b messenger RNA. It is suggested from the known distribution of this putative “end-of-messenger” signal, that it could play an essential part in controlling the expression of several minor proteins, both intronic and non-intronic. The possibility of the RF2 sequence being functional in spite of its interruptions is also discussed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...