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  • Springer  (180,681)
  • 2015-2019  (3,421)
  • 1970-1974  (115,206)
  • 1960-1964  (62,054)
  • 1
    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 2
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 3
    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 4
    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 5
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single Molecule FRET Methods to Study Glutamate Receptors -- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) -- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy -- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors -- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function -- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) -- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus -- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats -- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction -- Gene Expression Analysis by Multiplex Single Cell RT-PCR -- Metabolomics Analysis of Glutamate Receptor Function -- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation -- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function -- Fractionation of Subcellular Compartments from Human Brain Tissue -- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
    Abstract: This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications
    Pages: X, 260 p. 67 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990771
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  • 6
    Call number: WN180:8
    Keywords: Diagnostic Imaging ; Artificial intelligence
    Pages: xv, 373 p. : ill.
    ISBN: 9783319948775
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  • 7
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 8
    Cham : Springer
    Keywords: Immunology ; Emerging infectious diseases ; Immunology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: 1. Epidemiology of extrapulmonary tuberculosis -- 2. Pleural Tuberculosis -- 3. Gastrointestinal Tuberculosis -- 4. Hepatobiliary and splenic tuberculosis -- 5. Lymphatic Tuberculosis -- 6. Bone and Joint Tuberculosis -- 7. Tuberculous Spondylodiscitis -- 8. Tuberculous Meningitis -- 9. Tuberculous Encephalitis -- 10. Spinal tuberculosis -- 11. Genitourinary Tuberculosis -- 12. Cardiovascular Tuberculosis -- 13. Cutaneous Tuberculosis -- 14. Tuberculosis of Eye -- 15. Infection control in extrapulmonary TB -- 16. Tuberculosis in the ICU
    Abstract: This book brings awareness to a neglected condition that is nevertheless prevalent world-wide. Much focus is justly given to pulmonary tuberculosis, one of the key medical scourges of humanity, but this disease also often manifests itself in organs outside of the lungs. There is however a surprising lack of information available on extrapulmonary TB, which this book aims to remedy. Specifically, research, as well as epidemiology, diagnosis, and treatment options, are discussed in detail by an international list of experts. This comprehensive product serves as a valuable resource to numerous fields of medicine due to the presence of extrapulmonary tuberculosis throughout the human body
    Pages: VIII, 208 p. 7 illus., 1 illus. in color. : online resource.
    ISBN: 9783030047443
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  • 9
    Keywords: Biomedical Engineering ; Neurosciences ; Radiology, Medical ; Neurology ; Biomedical Engineering/Biotechnology ; Neurosciences ; Diagnostic Radiology ; Mathematical and Computational Biology ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Introduction -- Chapter 2. Multifractal study of EEG signal of subjects with Epilepsy and Alzheimer’s -- Chapter 3. Multifractal Approach for Quantification of Autonomic Deregulation due to Epileptic Seizure with ECG data -- Chapter 4. Multifractal Analysis of Electromyography data.-Chapter 5. Multifractal study on Parkinson’s and Huntington diseases with human gait data -- Chapter 6. Multifractal correlation study between posture and autonomic deregulation using ECG and blood pressure data -- Chapter 7. Appendix
    Abstract: This book primarily focuses on the study of various neurological disorders, including Parkinson’s (PD), Huntington (HD), Epilepsy, Alzheimer’s and Motor Neuron Diseases (MND) from a new perspective by analyzing the physiological signals associated with them using non-linear dynamics. The development of nonlinear methods has significantly helped to study complex nonlinear systems in detail by providing accurate and reliable information. The book provides a brief introduction to the central nervous system and its various disorders, their effects on health and quality of life, and their respective courses of treatment, followed by different bioelectrical signals like those detected by Electroencephalography (EEG), Electrocardiography (ECG), and Electromyography (EMG). In turn, the book discusses a range of nonlinear techniques, fractals, multifractals, and Higuchi’s Fractal Dimension (HFD), with mathematical examples and procedures. A review of studies conducted to date on neurological disorders like epilepsy, dementia, Parkinson’s, Huntington, Alzheimer’s, and Motor Neuron Diseases, which incorporate linear and nonlinear techniques, is also provided. The book subsequently presents new findings on neurological disorders of the central nervous system, namely Parkinson’s disease and Huntington’s disease, by analyzing their gait characteristics using a nonlinear fractal based technique: Multifractal Detrended Fluctuation Analysis (MFDFA). In closing, the book elaborates on several parameters that can be obtained from cross-correlation studies of ECG and blood pressure, and can be used as markers for neurological disorders
    Pages: XX, 182 p. 77 illus., 51 illus. in color. : online resource.
    ISBN: 9789811335525
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  • 10
    Keywords: Evolution (Biology) ; Life sciences ; Environmental sciences-Philosoph ; Biology-Philosophy ; Philosophy of nature ; Ecology ; Evolutionary Biology ; Popular Science in Nature and Environment ; Environmental Philosophy ; Philosophy of Biology ; Philosophy of nature ; Ecology ; Springer eBooks
    Description / Table of Contents: Introdution -- Between the Theoretical and the Hypothetical -- Taxonomic Uncertainties -- A Biosphere in Flux -- As Far as the Microscope Reveals -- Human Contradictions -- Varieties of Social Contracts -- Biological Consensus Mechanisms: The Future of Co-Existence
    Abstract: This book is a provocative and invigorating real-time exploration of the future of human evolution by two of the world’s leading interdisciplinary ecologists – Michael Charles Tobias and Jane Gray Morrison. Steeped in a rich multitude of the sciences and humanities, the book enshrines an elegant narrative that is highly empathetic, personal, scientifically wide-ranging and original. It focuses on the geo-positioning of the human Self and its corresponding species. The book's overarching viewpoints and poignant through-story examine and powerfully challenge concepts associated historically with assertions of human superiority over all other life forms. Ultimately, The Hypothetical Species: Variables of Human Evolution is a deeply considered treatise on the ecological and psychological state of humanity and her options – both within, and outside the rubrics of evolutionary research – for survival. This important work is beautifully presented with nearly 200 diverse illustrations, and is introduced with a foreword by famed paleobiologist, Dr. Melanie DeVore
    Pages: X, 318 p. 225 illus., 205 illus. in color. : online resource.
    ISBN: 9783030113193
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  • 11
    Keywords: Bioinformatics ; Genetics ; Human Genetics ; Agriculture ; Bioinformatics ; Genetics and Genomics ; Human Genetics ; Agriculture ; Springer eBooks
    Description / Table of Contents: Introduction to biological data -- Computers and bioinformatics -- Sequence biological databases -- Biological databases -- Other biological databases -- Nucleic acid sequencing -- Tools and methods in analysis of simple sequences -- Tools and methods in analysis of complex sequences -- Structural bioinformatics -- Protein structure annotations -- Introduction to functional bioinformatics -- Biological networks – tools, methods and analysis -- Metabolomics -- Drug discovery: concepts and approaches -- Drug designing methods and analysis -- Molecular molecular docking -- Insilico pcr -- Modeling and optimization of molecular bio-systems to generate predictive models -- Index
    Abstract: Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it mostly focuses on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. Unlike other bioinformatics books which are mostly theoretical, this book provides practical examples for the readers on state-of-the-art open source tools to solve biological problems. Flow charts of experiments, graphical illustrations, and mock data are included for quick reference. Volume I is therefore an ideal companion for students and early stage professionals wishing to master this blooming field
    Pages: XIV, 405 p. 193 illus., 186 illus. in color. : online resource.
    ISBN: 9783030026349
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  • 12
    Keywords: Human Physiology ; Human Physiology ; Springer eBooks
    Abstract: In recent years, volumes of the long-established Springer Subcellular Biochemistry series have shown great diversity of content, thus interesting biochemical topics, enthusiastically edited in all cases, have been readily accommodated. This new volume of the Subcellular Biochemistry series, Biochemistry and Cell Biology of Ageing: Part II, offers a broad overview of many different Clinical Science aspects of Ageing. The 17 chapters included in the book, contributed by knowledgeable authors, review many important topics at an advanced level. Whilst it has proved impossible to include all relevant topics, there is much present that will be of value to the reader. The book contains chapters on: “Poor Early Growth and Age-associated Disease; The Immune System and its Dysregulation with Ageing; Pulmonary Diseases and Ageing; Neurodegenerative Diseases and Ageing; Ageing and Cognition; Ageing and Osteoarthritis; Down Syndrome, Ageing and Epigenetics; The Vestibular System and Ageing; Signal Transduction, Ageing and Disease; Skin Changes During Ageing; Connective Tissue and Age-related Diseases ; Potential Cellular and Biochemical Mechanisms of Exercise and Physical Activity on the Ageing Process; Health Benefits of Anti-ageing Drugs; Visual Defects and Ageing; Hutchinson-Gilford Progeria Syndrome: Challenges at Bench and Bedside; Osteoporosis and the Ageing Skeleton; Neurovascular Ageing and Age-related Diseases” The book is primarily directed to advanced biomedical science and medical students, postgraduates, researchers, clinicians and academics in the field of Ageing. An earlier companion volume of the Subcellular Biochemistry series - Biochemistry and Cell Biology of Ageing: Part I (Volume 90) - covering the Biomedical Science aspects of ageing, has also been recently published
    Pages: X, 499 p. 53 illus., 47 illus. in color. : online resource.
    ISBN: 9789811336812
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  • 13
    Keywords: Mathematics ; Mathematics Education ; History of Mathematical Sciences ; International and Comparative Education ; Springer eBooks
    Description / Table of Contents: Chapter 1. European Didactic Traditions in Mathematics: Introduction and Overview -- Chapter 2. The French Didactic Tradition in Mathematics -- Chapter 3. Didactics of mathematics in the Netherlands -- Chapter 4. The Italian Didactic Tradition.-Chapter 5. The German Speaking Didactic Tradition -- Chapter 6. Didactics of Mathematics as a Research Field in Scandinavia -- Chapter 7. Czech and Slovak Research in Didactics of Mathematics Tradition and a Glance at Present State
    Abstract: This open access book discusses several didactic traditions in mathematics education in countries across Europe, including France, the Netherlands, Italy, Germany, the Czech and Slovakian Republics, and the Scandinavian states. It shows that while they all share common features both in the practice of learning and teaching at school and in research and development, they each have special features due to specific historical and cultural developments. The book also presents interesting historical facts about these didactic traditions, the theories and examples developed in these countries
    Pages: V, 212 p. 28 illus., 10 illus. in color. : online resource.
    ISBN: 9783030055141
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  • 14
    Keywords: Biochemistry ; Neurosciences ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease
    Abstract: This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects
    Pages: XII, 289 p. 66 illus., 55 illus. in color. : online resource.
    ISBN: 9781493991242
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  • 15
    Keywords: Cell Culture ; Laboratory animals ; Cell Culture ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Applicability of Mouse Models to the Study of Human Disease -- Optimizing the Cell Culture Microenvironment -- Propagation and Maintenance of Mouse Embryonic Stem Cells -- Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells -- Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction -- Gene Editing of Mouse Embryonic and Epiblast Stem Cells -- Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood -- Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture -- Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation -- Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo -- Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells -- Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells -- Organotypic Culture of Adult Mouse Retina -- Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes -- Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes -- Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells -- An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells -- Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans -- Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers -- The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential -- Mouse Lung Tissue Slice Culture
    Abstract: This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets
    Pages: XII, 315 p. 64 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990863
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  • 16
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism
    Abstract: This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories
    Pages: XI, 495 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990276
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  • 17
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 18
    Keywords: Cytology ; Neurosciences ; Cell Biology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System -- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing -- Myelin: Methods for Purification and Proteome Analysis -- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells -- Isolation and Culture of Oligodendrocytes -- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation -- Oligodendrocyte-Neuron Myelinating Co-Culture -- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier -- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices -- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord -- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination -- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination -- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation -- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis -- Conditional Mutagenesis in Oligodendrocyte Lineage Cells -- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage -- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination -- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease -- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes -- Transmission Electron Microscopy of Oligodendrocytes and Myelin -- Toxin-Based Models to Investigate Demyelination and Remyelination -- Magnetic Resonance techniques for Imaging White Matter
    Abstract: This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function
    Pages: XII, 411 p. 65 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990726
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  • 19
    Singapore : Springer
    Call number: QH324:49
    Keywords: Bioinformatics ; Medicine ; Statistics ; Biomedicine general ; Statistics for Life Sciences, Medicine, Health Sciences
    Description / Table of Contents: Part 1. BIOINFORMATICS FOR LIFE AND PERSONAL GENOME INTERPRETATION -- Chapter 1. Bioinformatics For Life -- Chapter 2. Next Generation Sequencing and Personal Genome Data Analysis -- Chapter 3. Personal Genome Data Analysis -- Chapter 4. Personal Genome Interpretation and Disease Risk Prediction -- Part 2. ADVANCED MICROARRAY DATA ANALYSIS -- Chapter 5. Advanced Microarray Data Analysis -- Chapter 6. Gene Expression Data Analysis -- Chapter 7. Gene Ontology and Biological Pathway-based Analysis -- Chapter 8. Gene-set Approaches and Prognostic Subgroup Prediction -- Chapter 9. MicroRNA Data Analysis -- Part 3. NETWORK BIOLOGY, SEQUENCE, PATHWAY AND ONTOLOGY INFORMATICS -- Chapter 10. Network Biology, Sequence, Pathway and Ontology Informatics -- Chapter 11. Motif and Regulatory Sequence Analysis -- Chapter 12. Molecular Pathways and Gene Ontology -- Chapter 13. Biological Network Analysis -- Part 4. SNPS, GWAS AND CNVS, INFORMATICS FOR GENOME VARIANTS -- Chapter 14. SNPs, GWAS, CNVs: Informatics for Human Genome Variations -- Chapter 15. SNP Data Analysis -- Chapter 16. GWAS Data Analysis -- Chapter 17. CNV Data Analysis -- Part 5. METAGENOME AND EPIGENOME, BASIC DATA ANALYSIS -- Chapter 18. Metagenome and Epigenome Data Analysis -- Chapter 19. Metagenome Data Analysis -- Chapter 20. Epigenome Databases and Tools -- Chapter 21. Epigenome Data Analysis -- Appendix A. BASIC PRACTICE USING R FOR DATA ANALYSIS -- Appendix B. APPLICATION PROGRAM FOR GENOME DATA ANALYSIS INSTALL GUIDE
    Abstract: This textbook describes recent advances in genomics and bioinformatics and provides numerous examples of genome data analysis that illustrate its relevance to real world problems and will improve the reader’s bioinformatics skills. Basic data preprocessing with normalization and filtering, primary pattern analysis, and machine learning algorithms using R and Python are demonstrated for gene-expression microarrays, genotyping microarrays, next-generation sequencing data, epigenomic data, and biological network and semantic analyses. In addition, detailed attention is devoted to integrative genomic data analysis, including multivariate data projection, gene-metabolic pathway mapping, automated biomolecular annotation, text mining of factual and literature databases, and integrated management of biomolecular databases. This textbook is primarily intended for life scientists, medical scientists, statisticians, data processing researchers, engineers, and other beginners in bioinformatics who are experiencing difficulty in approaching the field. However, it will also serve as a simple guideline for experts unfamiliar with the new, developing subfield of genomic analysis within bioinformatics
    Pages: xvi, 367 p. : ill.
    ISBN: 9789811319419
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  • 20
    Cham : Springer
    Keywords: Human Genetics ; Developmental Biology ; Immunology ; Medical virology ; Human Genetics ; Developmental Biology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Part 1 Foundations for muscle gene therapy -- Chapter 1. An overview of muscle biology and physiology for muscle gene therapy -- Chapter 2. Molecular basis of muscle disease -- Chapter 3. Animal models for muscle disease and muscle gene therapy -- Chapter 4. Muscle stem cell biology and implication in gene therapy -- Chapter 5. Pluripotent stem cells for gene therapy of hereditary muscle disorders -- Chapter 6. MicroRNAs (miRs) in muscle gene therapy -- Chapter 7. Immune system regulation of muscle injury and disease -- Chapter 8. Design of muscle gene therapy expression cassette -- Chapter 9 Non-viral vector mediated muscle gene therapy -- Chapter 10. Viral vectors for muscle gene therapy -- Chapter 11. Development of next generation muscle gene therapy AAV vectors -- Chapter 12. Histological and biochemical evaluation of muscle gene therapy -- Chapter 13. Optical polarization tractography imaging of structural changes in the skeletal and cardiac muscles of the mdx4cv mice -- Chapter 14. Biomarkers for muscle disease gene therapy -- Chapter 15. Large-scale clinical manufacturing of AAV Vectors for systemic muscle gene therapy -- Chapter 16. Genome-editing for muscle gene therapy -- Part 2 Preclinical muscle gene therapy -- Chapter 17. Considerations on preclinical muscle gene therapy studies -- Chapter 18. Gene replacement therapy for Duchenne muscular dystrophy -- Chapter 19. Recent advances in AON-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 20. AAV-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 21. Alternate translational initiation of dystrophin: a novel therapeutic approach -- Chapter 22. Genome editing for Duchenne muscular dystrophy -- Chapter 23. Sarcolipin knockdown therapy for Duchenne muscular dystrophy -- Chapter 24. Gene therapy for central nervous system in Duchenne muscular dystrophy -- Chapter 25. Therapeutic approach for dysferlinopathy in animal models -- Chapter 26. Muscle cell membrane repair and therapeutic implications -- Chapter 27. Treatment of muscular dystrophy-dystroglycanopathy: unlocking the potential of genetic engineering -- Chapter 28. RNAi therapy for dominant muscular dystrophies and other myopathies -- Chapter 29. Gene therapy for facioscapulohumeral muscular dystrophy (FSHD) -- Chapter 30. Gene therapy and gene editing for myotonic dystrophy -- Chapter 31. Gene therapy for oculopharyngeal muscular dystrophy -- Chapter 32. Gene therapy for X-linked myotubular myopathy -- Chapter 33. Pre-clinical gene therapy studies for metabolic myopathy -- Chapter 34 Elimination of mutant mitochondrial DNA in mitochondrial myopathies using gene editing enzymes -- Chapter 35. Gene therapy for Charcot-Marie-Tooth inherited neuropathy -- Chapter 36. Muscle - a potent target in vaccination -- Part 3 Clinical muscle gene therapy -- Chapter 37. Patient and family perspective on muscle gene therapy -- Chapter 38. Design of clinical trials for gene therapy in muscular dystrophy -- Chapter 39. Path to clinical trials: trial design, development of the clinical product and safety concerns in the implementation of clinical trials -- Chapter 40. Muscle MRI as an endpoint in clinical trials -- Chapter 41. Gene therapy clinical trials for Duchenne and limb girdle muscular dystrophies lessons learned -- Chapter 42. Duchenne muscular dystrophy exon-skipping trials -- Chapter 43. What we have learned from 10 years of DMD exon skipping trials -- Chapter 44. Clinical gene therapy trials for Pompe disease -- Chapter 45. Muscle-directed gene therapy for alpha-1 antitrypsin deficiency
    Abstract: About 7 million people worldwide are suffering from various inherited neuromuscular diseases. Gene therapy brings the hope of treating these diseases at their genetic roots. Muscle Gene Therapy is the only book dedicated to this topic. The first edition was published in 2010 when the field was just about to enter its prime time. The progress made since then has been unprecedented. The number of diseases that have been targeted by gene therapy has increased tremendously. The gene therapy toolbox is expanded greatly with many creative novel strategies (such as genome editing and therapy with disease-modifying genes). Most importantly, clinical benefits have begun to emerge in human patients. To reflect rapid advances in the field, we have compiled the second edition of Muscle Gene Therapy with contributions from experts that have conducted gene therapy studies either in animal models and/or in human patients. The new edition offers a much needed, up-to-date overview and perspective on the foundation and current status of neuromuscular disease gene therapy. It provides a framework to the development and regulatory approval of muscle gene therapy drugs in the upcoming years. This book is a must-have for anyone who is interested in neuromuscular disease gene therapy including those in the research arena (established investigators and trainees in the fields of clinical practice, veterinary medicine and basic biomedical sciences), funding and regulatory agencies, and patient community
    Pages: XVI, 811 p. 78 illus., 58 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030030957
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  • 21
    Keywords: Human Physiology ; Neurosciences ; Cytology ; Radiology, Medical ; Stem Cells ; Nanotechnology ; Human Physiology ; Neurosciences ; Oxidative Stress ; Diagnostic Radiology ; Stem Cells ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Cerebral stroke: An Introduction -- Chapter 2. Inflammation, oxidative stress, and neurodegeneration -- Chapter 3. Stroke induced blood brain barrier damage -- Chapter 4. Regulation of calcium ions in ischemic neuronal cell -- Chapter 5. Ischemic stroke induced endoplasmic reticulum stress -- Chapter 6. The role of autophagy in ischemic stroke: friend or foe?- Chapter 7. Critical role of mitochondrial autophagy in cerebral stroke -- Chapter 8. Application of neuroimaging in the identification of the pinpoint location of blockage -- Chapter 9. Emerging role of the electromagnetic field in stroke -- Chapter 10. Stem cell therapies for stroke -- Chapter 11. MicroRNA: Significance to stroke diagnosis, prognosis, and therapy -- Chapter 12. Therapeutic Aspects of Nanomedicines in Stroke Treatment -- Chapter 13. Neuroprotective potential of small molecule phytochemicals against stroke -- Chapter 14. Role of UV irradiation on neuroprotective potential of phytochemicals -- Chapter 15. Post-stroke treatment strategies, management, and rehabilitation
    Abstract: This book provides detailed and comprehensive mechanistic insights of the various risk factors that lead to the ischemic stroke and the novel therapeutic interventions against it. The first section discusses the different ischemic cerebral stroke-induced inflammatory pathways and dysfunctionality of blood-brain barrier. The later sections of the book deals with the role of endoplasmic reticulum stress and mitophagy in cerebral stroke and introduces the different neuroimaging techniques such as Computed tomography (CT), Magnetic resonance imaging (MRI), Positron emission tomography (PET) and Single-Photon emission computed tomography (SPECT) that are used to identify the arterial blockages. The final section comprises of chapters that focus on various neuroprotective strategies and emerging therapeutic interventions for combating stroke pathophysiology. The chapters cover the role of stem cell therapy, the therapeutic effect of low-frequency electromagnetic radiations (LF-EMR), and implications of non-coding RNAs such as micro-RNAs as the biomarkers for diagnosis, prognosis, and therapy in ischemic stroke
    Pages: VIII, 189 p. 23 illus., 12 illus. in color. : online resource.
    ISBN: 9789811314537
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  • 22
    Keywords: Pharmaceutical technology ; Pharmacy ; Pharmaceutical Sciences/Technology ; Pharmacy ; Springer eBooks
    Description / Table of Contents: Evolving Challenges in Developing Naturally-Derived Complex Mixtures into Drugs: U.S. Experience and Perspective -- Regulatory Landscapes for Approval of Naturally-derived Complex Mixture Drugs -- Complex Natural Product Heparin: Biosynthesis, Biology and Application via Synthetic Heparins -- Traditional Chinese Medicine Herbal Drugs: From Heritage to Future Developments -- Analytics on Farm-Dust Extract for Development of Novel Strategies to Prevent Asthma and Allergic Disease -- Analytical Tools for Physicochemical Characterization and Fingerprinting -- NMR in the Characterization of Complex Mixture Drugs -- Mass Spectrometry in the Characterization of Complex Drugs -- Scientific Considerations in the Approval of Complex Generi -- Raw Materials Production and Manufacturing Process Control Strategies -- Heparin Contamination and Issues Related to Raw Materials and Controls -- Pharmacology and Toxicology Concerns of Impurities -- Immunogenicity risks for Naturally Derived Complex Drugs -- Development of Plant-Derived Mixtures as Botanical Drugs: Clinical Considerations -- A Totality-of-Evidence Approach to Ensuring Therapeutic Consistency of Naturally Derived Complex Mixtures -- Economic Considerations for Complex Mixture Drugs -- Drug Discovery from Complex Mixtures: Serendipity, Screening and Characterization -- Integrated Approach for Characterization of Highly Heterogeneous Drugs
    Abstract: This volume in the AAPS Advances series covers various quality, safety and clinical aspects of drug development that are relevant to new and/or generic drugs containing a complex mixture of molecules. Specific topics discussed include: raw materials sourcing; manufacturing controls; characterization; identification of critical product quality components and attributes; identification of impurities, particularly as they bear on toxicity and immunogenicity; clinical trial study design considerations, and the regulatory science applications to development of such complex mixtures. Complex mixtures are challenging to characterize and analyze using standard methods. Further challenges extend throughout the product development cycle from raw material control to clinical study design. The regulatory landscape is rapidly changing as new types of complex mixtures are introduced into clinical trials and to the market (e.g., traditional Chinese medicines and medical marijuana products), while older products are facing generic competition for the first time (e.g., enoxaparin). The future outlook for complex generic drug products, as opposed to the more commonly developed targeted single agent drug products is not clear. The risks pertaining to lack of a full understanding of raw material control, process and controls in manufacture, as well as characterization of a complex mixture were seen vividly during the heparin crisis of 2008. As such powerful lessons have been learned about the regulatory science specific to complex products. The Science and Regulations of Naturally Derived Complex Drugs addresses the interests among industry, academics, and government on the issues surrounding the future development of mixtures for medicinal use
    Pages: VI, 331 p. 61 illus., 41 illus. in color. : online resource.
    ISBN: 9783030117511
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  • 23
    Keywords: Human Physiology ; Cardiovascular System ; Immunology ; Developmental Biology ; Biomedical Engineering ; Human Physiology ; Cardiovascular Biology ; Immunology ; Developmental Biology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: 1. Morphological and Functional Characteristics of Blood and Lymphatic Vessels -- 2. The Heart: The Engine in the Center of the Vascular System -- 3. Regulation of Tissue Perfusion and Exchange of Solutes, Macromolecules, and Water between Blood Vessels and the Interstitial Space -- 4. Endothelial Cells: Function and Dysfunction -- 5. Vascular Smooth muscle cells: Regulation of Vasoconstriction and Vasodilation -- 6. Embryonic Development of the Cardiovascular System -- 7. Cellular and Molecular Mechanisms of Vasculogenesis, Angiogenesis, and Lymphangiogenesis -- 8. Mechanisms of Hemostasis: Contribution of Platelets, Coagulation Factors, and the Vessel Wall -- 9. Biologically active lipids in Vascular Biology -- 10. Atherosclerosis -- 11. Venous Thromboembolism -- 12. Genetics of Vascular Diseases -- 13. Animal Models in Cardio-Vascular Biology -- 14. Endothelial Cell Isolation and Manipulation -- 15. In vitro Assays Used to Analyse Vascular Cell Functions -- 16. The Porcine Coronary Artery Ring Myograph System -- 17. Proteomics in Vascular Biology
    Abstract: This well-structured textbook offers essential knowledge on the vascular system. The reader will learn the properties, basic cellular mechanisms and development of the different parts of the vascular system (including the heart), gain knowledge on vascular and related diseases, and will be made familiar with common and most current methods and techniques applied to analyze the vascular system in patients, in animal models, and ex vivo. This book is based on a PhD Course for students from various bioscientific backgrounds given at the Medical University of Vienna, and it will be a valuable resource for Master´s Students in vascular biology and biomedicine in general and a helpful tool for young researchers world-wide wishing to gain or refresh their knowledge in this field
    Pages: XX, 392 p. 73 illus., 70 illus. in color. : online resource.
    ISBN: 9783030122706
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  • 24
    Keywords: Biochemistry ; Toxicology ; Neurobiology ; Protein Science ; Pharmacology/Toxicology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics -- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs -- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies -- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties -- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions -- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands -- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs -- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology -- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches -- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays -- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions -- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters -- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays -- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay -- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry -- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy -- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies -- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy -- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy -- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug -- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice -- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents -- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs
    Abstract: This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via
    Pages: XV, 406 p. 87 illus., 71 illus. in color. : online resource.
    ISBN: 9781493991211
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  • 25
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: 1. Classification of protein disulfide bonds -- 2. Assessing the evolutionary conservation of protein disulphide bonds -- 3. A proteomics workflow for the Identification of labile disulphide bonds at the cell surface -- 4. Quantification of the redox state of protein disulfide bonds -- 5. Quantification of the redox state of protein disulfide bonds -- 6. Studying functional disulfide bonds by computer simulations -- 7. Dynamic force spectroscopy analysis on the redox states of protein disulfide bonds -- 8. Assays of thiol isomerase enzymatic activity -- 9. Functional assays of thiol isomerase ERp5 -- 10. IDENTIFICATION OF PDI SUBSTRATES BY MECHANISM-BASED KINETIC TRAPPING -- 11. Site-specific proteomic mapping of modified cysteine residues -- 12. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry -- 13. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry -- 14. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide -- 15. Methodology for identification of the cysteine-reactive covalent inhibitors -- 16. In Vivo Measurement of Redox-Regulated TG2 Activity -- 17. Quantitation of total and free thiol β2-glycoprotein I levels for diagnostic and prognostic purposes in the antiphospholipid syndrome -- 18. Quantitation of total and free thiol angiotensinogen as a prognostic marker for pre-eclampsia -- 19. Preparation of a dithiol-reactive probe for PET imaging of cell death -- 20. Flow Cytometry Assessment of Procoagulant Platelets Using A Dithiol Reactive Probe
    Abstract: This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study
    Pages: XI, 327 p. 124 illus., 69 illus. in color. : online resource.
    ISBN: 9781493991877
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  • 26
    Keywords: Stem Cells ; Biology / Data processing ; Bioinformatics ; Stem Cells ; Computer Appl. in Life Sciences ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: Agent-Based Modeling to Delineate Spatiotemporal Control Mechanisms of the Stem Cell Niche -- Modeling Cellular Differentiation and Reprogramming with Gene Regulatory Networks -- Cell Population Model to Track Stochastic Cellular Decision-Making during Differentiation -- Automated Formal Reasoning to Uncover Molecular Programs of Self-Renewal -- Mathematical Modeling of Clonal Stem Cell Dynamics -- Computational Tools for Quantifying Concordance in Single Cell Fate -- Quantitative Modeling of the Waddington Epigenetic Landscape -- Modeling Gene Networks to Understand Multistability in Stem Cells -- Trajectory Algorithms to Infer Stem Cell Fate Decisions -- Gene Regulatory Networks from Single Cell Data for Exploring Cell Fate Decisions -- Reconstructing Gene Regulatory Networks that Control Hematopoietic Commitment -- Investigating Cell Fate Decisions with ICGS Analysis of Single Cells -- Lineage Inference and Stem Cell Identity Prediction using Single Cell RNA-Sequencing Data -- Dynamic Network Modeling of Stem Cell Metabolism -- Metabolomics in Stem Cell Biology Research -- Molecular Interaction Networks to Select Factors for Cell Conversion -- A Computational Approach for Cell Fate Reprogramming -- Computational Analysis of Aneuploidy in Pluripotent Stem Cells -- Cell Fate Engineering Tools for iPSC Disease Modeling
    Abstract: This volume details methods and protocols to further the study of stem cells within the computational stem cell biology (CSCB) field. Chapters are divided into four sections covering the theory and practice of modeling of stem cell behavior, analyzing single cell genome-scale measurements, reconstructing gene regulatory networks, and metabolomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Stem Cell Biology: Methods and Protocols will be an invaluable guide to researchers as they explore stem cells from the perspective of computational biology
    Pages: XI, 456 p. 107 illus., 95 illus. in color. : online resource.
    ISBN: 9781493992249
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  • 27
    Keywords: Biochemistry ; Pharmaceutical technology ; Biochemistry, general ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels -- Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6 -- Cryo-Electron Microscopy of TRP Channels -- Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation -- Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels -- Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique -- Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels -- High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons -- Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels -- TRP Channels Reconstitution in Lipid Bilayers -- Methods for Investigating TRP Channel Gating -- In Silico Approaches for TRP Channel Modulation -- Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction -- In Vivo Methods to Study ThermoTRP Channels in Rodents
    Abstract: This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels
    Pages: X, 238 p. 69 illus., 58 illus. in color. : online resource.
    ISBN: 9781493994465
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  • 28
    Keywords: Bioinformatics ; Medical genetics ; Bioinformatics ; Gene Function ; Springer eBooks
    Description / Table of Contents: tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences -- Predicting RNA Families In Nucleotides Sequences Using StructRNAfinder -- Structural and Functional Annotation of Eukaryotic Genomes with GenSAS -- Practical Guide for Fungal Gene Prediction from Genome Assembly and RNA-seq Reads by FunGAP -- Whole-Genome Annotation with BRAKER -- EuGene: An Automated Integrative Gene Finder for Eukaryotes and Prokaryotes -- ChemGenome2.1: An ab initio Gene Prediction Software -- Multi-Genome Annotation with AUGUSTUS -- GeMoMa: Homology-Based Gene Prediction Utilizing Intron Position Conservation and RNA-seq Data -- Coding Exon-Structure Aware Realigner (CESAR): Utilizing Genome Alignments for Comparative Gene Annotation -- Predicting Genes in Closely Related Species with Scipio and WebScipio -- AnABlast, Re-Searching for Protein-Coding Sequences in Genomic Regions -- Generating Publication-Ready Prokaryotic Genome Annotations with DFAST -- BUSCO: Assessing Genome Assembly and Annotation Completeness -- Evaluating Genome Assemblies and Gene Models using gVolante -- Choosing the Best Gene Predictions with GeneValidator -- COGNATE: Comparative Gene Annotation Characterizer
    Abstract: This volume introduces software used for gene prediction with focus on eukaryotic genomes. The chapters in this book describe software and web server usage as applied in common use-cases, and explain ways to simplify re-annotation of long available genome assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary computational requirements, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Gene Prediction: Methods and Protocols is a valuable resource for researchers and research groups working on the assembly and annotation of single species or small groups of species. Chapter 3 is available open access under a CC BY 4.0 license via
    Pages: XI, 284 p. 54 illus., 49 illus. in color. : online resource.
    ISBN: 9781493991730
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  • 29
    Keywords: Biochemistry ; Gene Expression ; Post-translational modification ; Protein Science ; Gene Expression ; Posttranslational Modification ; Springer eBooks
    Description / Table of Contents: High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets -- Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry -- Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty -- Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC -- Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo -- Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments -- Lysine Acetylation of Proteins and its Characterization in Human Systems -- Molecular and Cellular Characterization of SIRT1 Allosteric Activators -- Assessment of SIRT2 Inhibitors in Mouse Models of Cancer -- Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair -- Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes -- Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation -- Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons -- Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability -- Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling -- Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics -- Functional Analysis of HDACs in Tumorigeneis
    Abstract: The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems
    Pages: XIII, 310 p. 52 illus., 40 illus. in color. : online resource.
    ISBN: 9781493994342
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  • 30
    Keywords: Biochemistry ; Mass spectrometry ; Protein Science ; Mass spectrometry ; Springer eBooks
    Description / Table of Contents: Next Generation Proteomics for Clinical Biomarker Detection using SWATH-MS -- A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification of Protein Ubiquitination -- Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometry -- Quantitative Phosphoproteomic using Titanium Dioxide Micro-Columns and Label Free Quantitation -- Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond -- Quantitative Analysis of Protein S-Acylation Site Dynamics Using Site-Specific Acyl-Biotin Exchange (ssABE) -- Reducing Complexity? Cysteine Reduction and S-alkylation in Proteomic Workflows: Practical Considerations -- Detection of Unknown Chemical Adduct Modifications on Proteins: From Wet to Dry Laboratory -- Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry -- Metaproteomics of Freshwater Microbial Communities -- Peptide-to-Protein Summarization: An Important Step for Accurate Quantification In Label-Based Proteomics -- Experimental Design in Quantitative Proteomics -- Practical Integration of Multi-Run iTRAQ Data -- Quantitative Proteomics Data in the Public Domain: Challenges and Opportunities -- Computational Proteomics with Jupyter and Python -- The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis
    Abstract: New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIII, 264 p. 54 illus., 35 illus. in color. : online resource.
    ISBN: 9781493992324
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  • 31
    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 32
    New York, NY : Springer
    Keywords: Neurosciences ; Cell Culture ; Neurosciences ; Cell Culture ; Springer eBooks
    Description / Table of Contents: In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model -- In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models -- Oxidative Stress Signatures in Human Stem Cell Derived Neurons -- Glial Reactivity in Response to Neurotoxins: Relevance and Methods -- Neuron-Glia Interaction Studied with In Vitro Co-Culture -- Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria -- Epigenetic Changes in Cultured Neurons and Astrocytes -- The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation -- Zebrafish as a Tool to Assess Developmental Neurotoxicity -- Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies -- Electrophysiological Neuromethodologies -- A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies -- Transporter Studies: Brain Punching Technique -- MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders -- Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes
    Abstract: This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field
    Pages: XIII, 302 p. 57 illus., 47 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992287
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  • 33
    New York, NY : Springer
    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp
    Abstract: This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide
    Pages: XIII, 370 p. 77 illus., 60 illus. in color. : online resource.
    ISBN: 9781493992102
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  • 34
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Advancements in Managing Intracerebral Hemorrhage: Transition from Nihilism to Optimism -- Mandibular Advancement Devices in Patients with Symptoms of Obstructive Sleep Apnea: A Review -- Online Health Technologies and Mobile Devices: Attitudes, Needs and Future -- Radiological Response and Neutrophil-to-Lymphocyte Ratio as Predictive Factors for Progression-Free and Overall Survival in Metastatic Renal Cell Carcinoma Patients Treated with Sunitinib -- Plasminogen Activator Inhibitor Type 1 in Blood at Onset of Chemotherapy Unfavorably Affects Survival in Primary Ovarian Cancer -- Sialidase Attenuates Epidermal Growth Factor Response and Abolishes Antiproliferative Effects of Erlotinib in A549Alveolar Epithelial Cells -- Systemic Sclerosis and Serum Content of Transforming Growth Factor -- Protein-Bound Solute Clearance during Hemodialysis -- Mold Sensitization in Asthmatic and Non-Asthmatic Subjects Diagnosed with Extract-Based versus Component-Based Allergens -- Fructose Consumption and Lipid Metabolism in Obese Children and Adolescents -- Vaccination against Measles, Mumps, and Rubella in the Light of Current Epidemic Threats: Unjustified Postponement -- Variability of Dry-Eye Disease Following Removal of Lacrimal Glands in Rats
    Abstract: This book shares the latest research and practice-oriented findings in medical sciences with a wide audience. It addresses a range of contemporary issues, often unresolved or contentious, across various medical fields, including advances in the management of hemorrhagic brain stroke. It also discusses metastatic renal cell carcinoma – a global scourge with an extremely poor long-term survival prognosis, the course and sequelae of renal cell carcinoma, as well as advances in targeted molecular therapy with sunitinib, a receptor tyrosine kinase inhibitor. Further, it examines the molecular targeting of proliferative signaling of the epidermal growth factor receptor in the first-line treatment of patients with metastatic non-small-cell lung cancer. Other articles cover clearance of toxins in hemodialyzed patients; the search for diagnostic and therapeutic markers in the connective tissue disease scleroderma; obesity linked to inappropriate dietary habit; clinical problems related to the diagnosis of sensitization to fungi and its role in asthma; and reasons for the perilous trend of avoiding basic vaccinations in children. Lastly, the book explores the rapid developments in e-health technologies that increase access to health services, particularly for the elderly. The book is intended for clinical specialists, researchers, and all allied health professionals from various fields
    Pages: VI, 115 p. 17 illus., 10 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030190590
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  • 35
    Keywords: Oncology ; Human Genetics ; Database management ; Data mining ; Cancer Research ; Human Genetics ; Database management ; Data Mining and Knowledge Discovery ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction -- Resources -- Data Retrieval -- Text Processing -- Semantic processing -- Index
    Abstract: This open access book is a step-by-step introduction on how shell scripting can help solve many of the data processing tasks that Health and Life specialists face everyday with minimal software dependencies. The examples presented in the book show how simple command line tools can be used and combined to retrieve data and text from web resources, to filter and mine literature, and to explore the semantics encoded in biomedical ontologies. To store data this book relies on open standard text file formats, such as TSV, CSV, XML, and OWL, that can be open by any text editor or spreadsheet application. The first two chapters, Introduction and Resources, provide a brief introduction to the shell scripting and describe popular data resources in Health and Life Sciences. The third chapter, Data Retrieval, starts by introducing a common data processing task that involves multiple data resources. Then, this chapter explains how to automate each step of that task by introducing the required commands line tools one by one. The fourth chapter, Text Processing, shows how to filter and analyze text by using simple string matching techniques and regular expressions. The last chapter, Semantic Processing, shows how XPath queries and shell scripting is able to process complex data, such as the graphs used to specify ontologies. Besides being almost immutable for more than four decades and being available in most of our personal computers, shell scripting is relatively easy to learn by Health and Life specialists as a sequence of independent commands. Comprehending them is like conducting a new laboratory protocol by testing and understanding its procedural steps and variables, and combining their intermediate results. Thus, this book is particularly relevant to Health and Life specialists or students that want to easily learn how to process data and text, and which in return may facilitate and inspire them to acquire deeper bioinformatics skills in the future
    Pages: XV, 98 p. 483 illus., 74 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030138455
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  • 36
    Keywords: Pharmaceutical technology ; Biomedical Engineering ; Biochemical engineering ; Pharmaceutical Sciences/Technology ; Biomedical Engineering/Biotechnology ; Biochemical engineering ; Springer eBooks
    Description / Table of Contents: Introduction -- Concept of spherical crystallization -- Paradigm shifted pharmaceutical process introduced by spherical crystallization -- Particulate-design platform developed by the Advanced Spherical Crystallization System -- Development of Novel Microsphere and Microballoon DDS by Polymeric Spherical Crystallization -- Development of PLGA Nanopsphere for Drug Delivery System (DDS) Prepared by Polymeric Spherical Crystallization -- Application of PLGA NSs to cosmetics -- Future perspectives of PLGA nanospheres for advanced DDSs and continuous preparation systems for spherical crystallizers
    Abstract: This book describes the principles and applications of the spherical crystallization technique, from the standpoint of its inventor. After an introduction on the history of particle design engineering and nanotechnology, the concept of spherical crystallization itself is clearly explained. Attention then turns to the application of spherical crystallization in pharmaceutical processes. It is explained how the technique can provide physicochemical properties suitable for direct tableting of active pharmaceutical ingredients and how it has enabled the development of a novel particulate design platform from single to complex system. Subsequent chapters describe the roles of polymeric spherical crystallization in the preparation of novel microspheres, microballoons for drug delivery systems (DDS) and the development of biocompatible and biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanospheres. The various applications of PLGA nanospheres composite within oral-, pulmonary-, transdermal DDS and cosmetics are fully discussed. Finally, future perspectives are presented on use of the technology in the design and industrial-scale manufacture of new drug delivery systems, highlighting how a continuous pharmaceutical process that meets US Food and Drug Administration quality requirements should soon be introduced
    Pages: XVII, 118 p. 110 illus., 32 illus. in color. : online resource.
    ISBN: 9789811367861
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  • 37
    Keywords: Cardiovascular System ; Biomedical Engineering ; Cardiology ; Medicine ; Cardiovascular Biology ; Biomedical Engineering/Biotechnology ; Biomedical Engineering and Bioengineering ; Cardiology ; Biomedicine general ; Springer eBooks
    Description / Table of Contents: Chapter 1: Biomechanics -- Chapter 2: Morphometry of Coronary Vasculature -- Chapter 3: Mechanical Properties and Microstructure of the Coronary Vasculature -- Chapter 4: Constitutive Models of Coronary Vasculature -- Chapter 5: Network Analysis of Coronary Circulation: I. Steady State Flow -- Chapter 6: Network Analysis of Coronary Circulation: II. Pulsatile Flow -- Chapter 7: Scaling Laws of Coronary Vasculature -- Chapter 8: Local Coronary Flow and Stress Distribution
    Abstract: This comprehensive text examines both global and local coronary blood flow based on morphometry and mechanical properties of the coronary vasculature. Using a biomechanical approach, this book addresses coronary circulation in a quantitative manner based on models rooted in experimental data that account for the various physical determinants of coronary blood flow including myocardial-vessel interactions and various mechanisms of autoregulation. This is the first text dedicated to a distributive analysis (as opposed to lumped) and provides digital files for detailed anatomical data (e.g., diameters, lengths, node-to-node connections) of the coronary vessels. This book also provides appendices with specific mathematical formulations for the biomechanical analyses and models in the text. Written by Dr. Ghassan S. Kassab, a leader in the field of coronary biomechanics, Coronary Circulation: Anatomy, Mechanical Properties, and Biomechanics is a synthesis of seminal topics in the field and is intended for clinicians, bioengineers, and researchers as a compendium on the topic. The detailed anatomical and mechanical data provided are intended to be used as a platform to address new questions in this exciting and clinically very important research area
    Pages: XVII, 564 p. 171 illus., 49 illus. in color. : online resource.
    ISBN: 9783030148195
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  • 38
    Keywords: Biochemistry ; Veterinary Medicine ; Nutrition ; Toxicology ; Animal Biochemistry ; Veterinary Medicine/Veterinary Science ; Nutrition ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Part I. Common Nutraceuticals -- Standardized Turmeric and Curcumin -- Fenugreek in Health and Disease -- Neem Extract -- Nutraceutical Potential of Ginger -- Berberine -- Sea Buckthorn and Apricot Based Nutraceuticals -- Nigella Sativa -- Babool (Acacia nilotica) -- Glucosinolates and Organosulfur Compounds -- Cannabis in Veterinary Medicine: Cannabinoid Therapies for Animals -- Essential Oils -- Omega Fatty Acids -- Polyphenols and Flavonoids -- Antioxidants in Prevention and Treatment of Diseases and Toxicity -- Resveratrol: Biological Activities and Potential Use in Health and Disease -- Egg Shell Membranes for Veterinary Uses -- Egg derived Ovotransferrins and Lactoferrins -- Colostrum Antibodies, Egg Antibodies and Monoclonal Antibodies Providing Passive Immunity for Animals -- Part II. Prebiotics, Probiotics, Synbiotics, and Antimicrobials -- Prebiotics and Probiotics in Feed and Animal Health -- Synbiotics in Animal Health and Production -- Enzymes in Feed and Animal Health -- Nutraceuticals Used as Antibacterial Alternatives in Animal Health and Disease -- Feed Additives in Animal Health -- Part III. Nutraceuticals in Organ- and System-Disorders -- Nutraceuticals in Arthritis -- Nutraceuticals for Antiaging -- Nutraceuticals for Cognitive Dysfunction -- Nutraceuticals for Calming and Stress -- Nutraceuticals in Cardiovascular Diseases -- Nutraceuticals in Hepatic and Pancreatic Diseases -- Nutraceuticals in Periodontal Health and Diseases in Dogs and Cats -- Nutraceuticals in Gastrointestinal Conditions -- Nutraceuticals in Reproductive Disorders -- Nutraceuticals in Genitourinary Maladies -- Nutraceuticals in Obesity and Metabolic Disorders -- Nutraceuticals for Diabetes in Dogs and Cats -- Nutraceuticals in Wound Healing: A Special Focus on Chromolaena Odorata as Guardian of Health with Broad Spectrum of Biological Activities -- Nutraceuticals in Dermatological Disorders -- Nutraceuticals in Mastitis -- Nutraceuticals in Immune Disorders -- Plant and Food Derived Immunomodulators as Nutraceuticals for Performance Enhancing Activities -- Nutraceuticals for the Prevention and Cure of Cancer -- Expanding Metabolic Targets in Cancer by Select Combinations of Vitamin C and EGCG with Different Natural Compounds -- Nutraceuticals for Control of Ticks, fleas and other Ectoparasites -- Part IV. Nutraceuticals in Specific Animal Species -- Nutraceuticals in Cattle Health and Diseases -- Nutraceuticals in Equine Medicine -- Nutraceuticals for Camelids -- Nutraceuticals in Poultry Health and Diseases -- Part V -- Safety and Toxicity Evaluation of Nutroceuticals and Functional Foods -- Safety and Toxicity Evaluation of Nutraceuticals in Animal Models -- Evaluation of Safety and Efficacy of Nutraceuticals using Drosophila as an in vivo Tool -- Biomarkers of Foods and Nutraceuticals: Applications in Efficacy, Safety and Therapy -- Toxicology and Drug Interactions of Nutraceuticals -- Part VI. Newer Trends in Nutraceutical Research and Product Development -- Proteomics in the Evaluation of Nutraceuticals and Functional Foods -- Nanoparticles and Molecular Delivery System for Nutraceuticals Bioavailability -- Nanosupplements and Animal Health -- Veterinary Nutraceuticals Stability Testing -- Part VII. Regulatory Aspects and Country-Specific Requirements for Nutraceuticals -- Basic Regulatory Guidelines for Veterinary Nutraceuticals -- Regulatory Aspects of Veterinary Nutraceuticals in the USA and Canada -- Regulatory Guidelines for Nutraceuticals in the European Union -- Regulatory Guidelines for Nutraceuticals and Food Supplements in India -- Uses and Regulatory Guidelines for Nutraceuticals in China -- Regulation of Nutraceuticals in Australia and New Zealand -- Regulatory Guidelines for Nutraceuticals and Dietary Supplements for Animals in Turkey -- Uses and Regulation of Nutraceuticals for Animals in the Philippines -- Regulatory Guidelines for Nutraceuticals in South Africa
    Abstract: This unique work compiles the latest knowledge around veterinary nutraceuticals, commonly referred to as dietary supplements, from ingredients to final products in a single source. More than sixty chapters organized in seven sections collate all related aspects of nutraceutical research in animal health and disease, among them many novel topics: common nutraceutical ingredients (Section-I), prebiotics, probiotics, synbiotics, enzymes and antibacterial alternatives (Section-II), applications of nutraceuticals in prevention and treatment of various diseases such as arthritis, periodontitis, diabetes, cognitive dysfunctions, mastitis, wounds, immune disorders, and cancer (Section-III), utilization of nutraceuticals in specific animal species (Section-IV), safety and toxicity evaluation of nutraceuticals and functional foods (Section-V), recent trends in nutraceutical research and product development (Section-VI), as well as regulatory aspects for nutraceuticals (Section-VII). The future of nutraceuticals and functional foods in veterinary medicine seems bright, as novel nutraceuticals will emerge and new uses of old agents will be discovered. International contributors to this book cover a variety of specialties in veterinary medicine, pharmacology, pharmacognosy, toxicology, chemistry, medicinal chemistry, biochemistry, physiology, nutrition, drug development, regulatory frameworks, and the nutraceutical industry. This is a highly informative and carefully presented book, providing scientific insight for academia, veterinarians, governmental and regulatory agencies with an interest in animal nutrition, complementary veterinary medicine, nutraceutical product development and research
    Pages: XIX, 877 p. 120 illus., 66 illus. in color. : online resource.
    ISBN: 9783030046248
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  • 39
    Keywords: Biochemistry ; Toxicology ; Entomology ; Biochemistry, general ; Pharmacology/Toxicology ; Entomology ; Springer eBooks
    Description / Table of Contents: An Introduction to the book -- Chitin: structure, chemistry & biology -- Chitin prevalence and function in bacteria, fungi and protists -- Immune responses of mammals and plants to chitin-containing pathogens -- Chitin organizing and modifying enzymes and proteins involved in remodeling of the insect cuticle -- Chitin-active lytic polysaccharide monooxygenases -- Bacterial Chitinase System as a Model of Chitin Biodegradation -- Chitin synthesis and degradation in fungi: biology and enzymes -- Chitin in Arthropods: Biosynthesis, Modification and Metabolism -- Nematode chitin and application -- Human Chitinases: Structure, Function and Inhibitor Discovery -- Chitin/chitosan-active enzymes involved in plant-microbe interactions -- Chitinous structures as potential targets for insect pest control
    Abstract: This book provides a comprehensive overview of chitin biology and chitin metabolism related enzymes. Chitin, the second most abundant biopolymer in nature after to cellulose, is a linear biopolymer composed of β-1,4-linked N-acetylglucosamine (GlcNAc), and an essential component in the exoskeletons of insects, mites, ticks and crustaceans, the egg shells of parasitic nematodes, and fungal cell walls. Although some chitin-containing organisms are a threat to human health, food safety and agricultural production, non-chitin containing organisms like humans, mammals and plants have an innate immune response to these hazardous organisms. The book provides researchers and students with information on the recent research advances concerning the biology of chitin-containing organisms as well as cross-talks between chitin-containing and non-chitin-containing organisms. Highlighting chitin remodeling enzymes and inhibitors, it also offers drug developers essential insights into designing specific molecules for the control of hazardous chitin-containing organisms
    Pages: VIII, 292 p. 65 illus., 26 illus. in color. : online resource.
    ISBN: 9789811373183
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  • 40
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Introduction -- Direct binding of cholesterol to proteins -- Cholesterol-recognition motifs in membrane proteins -- Molecular determinants of cholesterol binding to proteins: overview of crystallographic studies -- Crystallographic studies of steroid-protein interactions -- Insights into cholesterol binding sites in proteins from computational studies -- Cholesterol binding to NPC1/NPC2 -- I Biophysical elements of the interaction between cholesterol and the nicotinic acetylcholine receptor -- Modulation of TRPV1 channels by cholesterol -- Cholesterol binding sites in inwardly rectifying potassium channels
    Abstract: In this book, renowned scientists describe how cholesterol interacts with various proteins. Recent progress made in the high-resolution visualization of cholesterol-protein interactions using crystallography and cryogenic electron microscopy has substantially advanced the knowledge of critical features. These features enable specific recognition of the cholesterol molecule by proteins, a process that was built on earlier studies using binding assays, computational modeling and site-directed mutagenesis. Direct Mechanisms in Cholesterol Modulation of Protein Function offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via direct sensing. Its nine chapters are organized into two distinct parts. In the first part, the chapters introduce the reader to the general characteristics of cholesterol binding sites in proteins. This part starts with a tour into common cholesterol recognition motifs, followed by an overview of the major classes of steroid-binding proteins. It then continues with two chapters that present a comprehensive analysis of molecular and structural characteristics of cholesterol binding sites in transmembrane and soluble protein domains. In the second part of the book, examples of cholesterol binding sites and consequences of specific cholesterol recognition for protein function are presented for G protein-coupled receptors, ion channels and cholesterol-transporting proteins. The book is valuable for undergraduate and graduate students in biochemistry and nutrition, as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: IX, 168 p. 51 illus., 50 illus. in color. : online resource.
    ISBN: 9783030142650
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  • 41
    Keywords: Biomedical Engineering ; Oncology ; Biomedical Engineering/Biotechnology ; Mathematical Modeling and Industrial Mathematics ; Cancer Research ; Biomedical Engineering and Bioengineering ; Springer eBooks
    Description / Table of Contents: Fixed Boundary PDE Model Formulation -- Fixed Boundary PDE Model Implementation -- Fixed Boundary PDE Model Output -- Moving Boundary PDE Model Implementation -- Moving Boundary PDE Model Output -- Index
    Abstract: The focus of this book is a detailed discussion of a dual cancer vaccine (CV)-immune checkpoint inhibitor (ICI) mathematical model formulated as a system of partial differential equations (PDEs) defining the spatiotemporal distribution of cells and biochemicals during tumor growth. A computer implementation of the model is discussed in detail for the quantitative evaluation of CV-ICI therapy. The coding (programming) consists of a series of routines in R, a quality, open-source scientific computing system that is readily available from the internet. The routines are based on the method of lines (MOL), a general PDE algorithm that can be executed on modest computers within the basic R system. The reader can download and use the routines to confirm the model solutions reported in the book, then experiment with the model by varying the parameters and modifying/extending the equations, and even studying alternative models with the PDE methodology demonstrated by the CV-ICI model. Spatiotemporal Modeling of Cancer Immunotherapy: Partial Differential Equation Analysis in R facilitates the use of the model, and more generally, computer- based analysis of cancer immunotherapy mathematical models, as a step toward the development and quantitative evaluation of the immunotherapy approach to the treatment of cancer
    Pages: VIII, 112 p. 70 illus. : online resource.
    ISBN: 9783030190804
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  • 42
    Keywords: Microscopy ; Cytology ; Zoology ; Botany ; Biological Microscopy ; Popular Life Sciences ; Cell Biology ; Zoology ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Chapter 1:Introduction -- Chapter 2:The advantages of learning traditional microscopy -- Chapter 3:Buying a microscope -- Chapter 4:The science of light -- Chapter 5:Introduction to lenses -- Chapter 6:The anatomy of the microscope -- Chapter 7:The Eyepiece -- Chapter 8:The Objective -- Chapter 9:The Stage -- Chapter 10:The Condenser and Diaphragm -- Chapter 11:The Light source -- Chapter 12: Choosing the right technique -- Chapter 13:Sample prep -- Chapter 14:Image capture -- Chapter 15:Trouble-shooting
    Abstract: This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools
    Pages: XXI, 164 p. 51 illus., 43 illus. in color. : online resource.
    ISBN: 9783030053932
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  • 43
    Keywords: Stem Cells ; Oncology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Pericytes in cutaneous wound healing -- Pericytes in glioblastomas: multifaceted role within tumor microenvironments and potential for therapeutic interventions -- Pericytes in breast cancer -- Pericytes in sarcomas and other mesenchymal tumors -- Pericytes in metastasis -- The role of pericytes in Amyotrophic Lateral Sclerosis -- Pericytes in Alzheimer’s Disease: novel clues to cerebral amyloid angiopathy pathogenesis -- Pericytes in Multiple Sclerosis -- Pericytes in ischemic stroke -- Pericytes in Hereditary Hemorrhagic Telangiectasia -- Pericytes in primary familial brain calcification -- Pericytes in type 2 diabetes -- Pericytes in atherosclerosis -- Pericytes in chronic lung disease -- Pericytes in muscular dystrophies -- Index
    Abstract: This volume explores pericytes' roles under distinct pathological conditions, ranging from tumors, ALS, Alzheimer’s disease, Multiple Sclerosis, stroke, diabetes, atherosclerosis, muscular dystrophies and more. Together with its companion volumes Pericyte Biology in Different Organs and Pericyte Biology – Novel Concepts, Pericyte Biology in Disease presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cell biology and clinicians involved with these specific diseases
    Pages: XII, 355 p. 62 illus., 57 illus. in color. : online resource.
    ISBN: 9783030169084
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  • 44
    Keywords: Human Genetics ; Human Physiology ; Neurochemistry ; Medicine ; Biochemistry ; Cardiovascular System ; Human Genetics ; Human Physiology ; Neurochemistry ; Molecular Medicine ; Protein Science ; Cardiovascular Biology ; Springer eBooks
    Description / Table of Contents: Prelims -- Diffusion and transport of reactive species across cell membranes -- Characterization of hydroxy and hydroperoxy polyunsaturated fatty acids by mass spectrometry. Understanding Peroxisome Proliferator-Activated Receptors: from the structure to the regulatory actions on metabolism -- Diminishing Inflammation by Reducing Oxidant Generation: Nitrated Fatty Acid-Mediated Inactivation of Xanthine Oxidoreductase. Unfolded protein response: Cause or consequence of lipid and lipoprotein metabolism disturbances?- Arachidonic acid metabolism in macrophages: role of NADPH oxidases -- Lipid Metabolism and Signaling in Platelet Function -- Dyslipidemia in ischemia/reperfusion injury -- Epicardial adipose tissue in cardiovascular disease -- Bioactive Lipids and the gut-brain axis: diet as a modulator of bioactivity and diversity of lipids in the brain -- Nitroalkylation of α-synuclein by nitro-oleic acid: Implications for Parkinson´s disease -- Bioactive lipids in inflammation after central nervous system injury -- Index
    Abstract: The purpose of this book is to introduce the readers on the perspective of the role that unsaturated fatty acids and complex lipids play on health and disease. Bioactive lipids can be modified affecting membrane composition, structure and fluidity in addition to changes in cell signaling associated to lipid-protein (membrane receptors) interactions, issues that are addressed by the authors. This book analyzes key topics involving bioactive lipids and their role in normal signaling and the mechanisms of disease. The book navigates from structural studies of oxidized and non-oxidized lipids to the reactions and cell signaling processes that bioactive lipids play in cardiovascular and neurodegenerative diseases. The book contains the recent advances reported in the literature about lipidomics as well as the role that lipid-derived compounds exert on unfolded protein response and lipid metabolism and disease. This book represents a state of the art introduction to lipid metabolism from a biochemical to an in vivo overview being an useful tool for students and investigators. We hope the mechanistic observations on the role of bioactive lipids in health and disease serve a perspective to improve the existing treatments or propose new lipid-based pharmacology
    Pages: VIII, 198 p. 41 illus., 35 illus. in color. : online resource.
    ISBN: 9783030114886
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  • 45
    Keywords: Medicine ; Pharmaceutical technology ; Biomedical Engineering ; Diabetes ; Molecular Medicine ; Pharmaceutical Sciences/Technology ; Biomedical Engineering/Biotechnology ; Diabetes ; Springer eBooks
    Description / Table of Contents: Chapter 1. Improvement of HDL -- 1-1.Clinical application of HDL: Overview -- 1-2.Regression effect of V156K-apoA-I -- 1-3.Anti-diabetic effect of V156K-apoA-I -- 1-4.Vitamin C and HDL -- 1-5.Growth hormone-1 in HDL -- 1-6.Growth hormone-2 in HDL -- 1-7.Omega-3 and HDL -- Chapter 2. HDL as therapeutic tools -- 2-1.Delivery vehicle for therapeutics : Overview -- 2-2.Gene delivery: adenovirus and V156K-apoA-I -- 2-3.Solubilization of rapamycin and delivery -- 2-4.Solubilization of minoxidil and delivery -- Chapter 3. Enhancement of HDL by policosanol: anti-aging and longevity -- 3-1.Policosanol and HDL functionality -- 3-2.Policosanol and raising HDL in zebrafish -- 3-3.Policosanol and CETP inhibition: human trial 8 weeks -- 3-4.Policosanol and blood pressure lowering: human trial 8 weeks -- 3-5.Policosanol blood pressure lowering human trial for 24 weeks -- 3-6.Policosanol and improvement of aortic stiffness -- 3-7.Policosanol and blood pressure lowering in rat model -- 3-8.Twelve week consumption of policosanol and lowering blood pressure -- 3-9.Publication analysis of policosanol-related studies -- 3-10.Athero-protective effects of policosanol and sugar cane wax acid -- 3-11.Cuban sugarcane wax acids and policosanol improved serum lipid profiles -- Summary and perspectives -- Epilogue
    Abstract: This book is the second of two volumes that offer a comprehensive, up-to-date account of current knowledge regarding high-density lipoprotein (HDL), the changes that occur in HDL under different conditions, the clinical applications of HDL, and means of enhancing HDL functionality. In this volume, the focus is on the improvement of HDL, enhancement of its functionality, and the use of HDL for therapeutic purposes. In the first section, up-to-date information is provided on such topics as the tumor regression-promoting and antidiabetic activities of reconstituted HDL containing V156K apolipoprotein A-I, the enhancement of HDL effects by high doses of vitamin C, the benefits derived from incorporation of growth hormones 1 and 2 into rHDL, and the biological functions of omega-3 linolenic acid in rHDL. The enhancement of HDL functionality by policosanol and the resultant benefits are thoroughly examined in a separate section. Readers will also find the latest information on clinical applications of HDL. Here, specific topics include the enhancement of adenoviral gene delivery and the delivery of rapamycin. In documenting the latest knowledge in this field, this volume will be of interest to both researchers and clinicians
    Pages: XVI, 268 p. 174 illus. : online resource.
    ISBN: 9789811373831
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  • 46
    Keywords: Cell Culture ; Regenerative Medicine/Tissue Engineering ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Part 1 : Isolation and culture of skin cells -- Chap 1: Isolation and Culture of Human Keratinocytes -- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture -- Chap 3 : Isolation and Culture of Epidermal Melanocytes -- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor -- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells -- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts -- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells -- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue -- Part 2 : Tissue engineering skin and engineered skin models -- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility -- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes -- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells -- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes -- Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering -- Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models -- Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin -- Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts -- Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy -- Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo -- Chap 18 : Human Reconstructed Skin in a Mouse Model -- Chap 19 : Pig Model to Test Tissue Engineered Skin -- Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model
    Abstract: This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine
    Pages: XII, 263 p. 79 illus., 66 illus. in color. : online resource.
    ISBN: 9781493994731
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  • 47
    Keywords: Metabolism ; Mass spectrometry ; Metabolomics ; Mass spectrometry ; Springer eBooks
    Description / Table of Contents: Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores by Mass Spectrometry -- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer -- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification -- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites -- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells -- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson’s Disease -- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease -- Proteometabolomics of Melphalan Resistance in Multiple Myeloma -- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading -- Protocols for NMR Analysis in Livestock Metabolomics -- Metabolomics Applicable to Retinal Vascular Diseases
    Abstract: The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field
    Pages: XV, 335 p. 82 illus., 53 illus. in color. : online resource.
    ISBN: 9781493994885
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  • 48
    Keywords: Toxicology ; Stem Cells ; Pharmacology/Toxicology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Cell-Based Assays using Differentiated Human Induced Pluripotent Cells -- Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells -- Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modelling -- Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation -- Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies -- Improved Protocol of Cardiac Differentiation and Maturation of Pluripotent Stem Cells -- Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors -- A Protocol for the One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes -- Differentiation of hiPS Cells into Definitive Endoderm for High Throughput Screening -- LC-MS/MS Based Metabolomics for Cell Cultures -- Preparation of iPSCs for Targeted Proteomic Analysis -- Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes -- Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation -- Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates -- High Content Autophagy Analysis in iPSC-Derived Neurons using Immuno-Fluorescence -- High Content Analysis of Mitochondrial Function in iPSC-Derived Neurons -- Evaluating the Effect of Drug Compounds on Cardiac Spheroids using the Cardiac Cell Outgrowth Assay -- Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well Microelectrode Arrays -- Multielectrode Array (MEA) Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons -- Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm -- Fabrication of a Microfluidic Cell Culture Device using Photolithographic and Soft Lithographic Techniques -- Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells -- hiPS Cell–Derived Neurons for High-Throughput Screening
    Abstract: The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study
    Pages: XIV, 266 p. 69 illus., 55 illus. in color. : online resource.
    ISBN: 9781493994779
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  • 49
    Keywords: Bioinformatics ; DNA microarrays ; Genetics ; Bioinformatics ; Microarrays ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: 1. Introduction to bioinformatics -- 2. Protocol for DNA microarrays on glass slides -- 3. Data warehousing with TargetMine for omics data analysis -- 4. A review of microarray datasets: where to find them and specific characteristics -- 5. Statistical analysis of microarray data -- 6. Feature selection applied to microarray data -- 7. Cluster analysis of microarray data -- 8. Classification of microarray data -- 9. Microarray data normalization and robust detection of rhythmic features -- 10. HPC tools to deal with microarray data -- 11. ROC curves for the statistical analysis of microarray data -- 12. Missing values imputation algorithms for microarray gene expression data -- 13. Computer tools to analyze microarray data -- 14. Challenges and future trends for microarray analysis
    Abstract: This book provides a comprehensive, interdisciplinary collection of the main, up-to-date methods, tools, and techniques for microarray data analysis, covering the necessary steps for the acquisition of the data, its preprocessing, and its posterior analysis. Featuring perspectives from biology, computer science, and statistics, the volume explores machine learning methods such as clustering, feature selection, classification, data normalization, and missing value imputation, as well as the statistical analysis of the data and the most popular computer tools to analyze microarray data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will aid researchers in getting successful results. Cutting-edge and authoritative, Microarray Bioinformatics serves as an ideal guide for researchers and graduate students in bioinformatics, with basic knowledge in biology and computer science, and with a view to work with microarray datasets
    Pages: X, 299 p. 60 illus., 52 illus. in color. : online resource.
    ISBN: 9781493994427
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  • 50
    Keywords: Toxicology ; Medicine ; Pharmacology/Toxicology ; Molecular Medicine ; Biomedicine general ; Springer Nature Living Reference
    Description / Table of Contents: From the Contents: ABC Transporters -- Absorption -- Abstinence -- Abuse -- Abuse Liability -- Acamprosate -- BAC -- Balanced Placebo Design -- Barbital -- Barbiturates -- Basal Forebrain Cholinergic Neurons -- Behavioral Allocation Function -- Cabergoline -- Cachexia -- Caffeine -- Caffeine Withdrawal Syndrome -- Caffeinism -- cAMP -- DARPP-32 -- db/db Mouse -- D-cycloserine -- Decision Making -- Declarative and Non-Declarative Memory -- Declarative Memory -- Early Ventral Hippocampal Lesion Model -- Eating and Appetite -- Eating Disorder: Anorexia Nervosa -- Eating Disorders: Animal Models -- Eating Disorders: Binge-Eating -- Echolalia -- FAA -- Face Validity -- Facial Dysmorphology -- False Memory -- False Negative -- False Positive -- G protein-Coupled Receptors -- GABA -- GABAA Receptor -- GABAergic Transmission -- Gabapentin -- Galantamine -- Habit Reversal Therapy -- Habit Reversal Training -- Habituation -- Half-Life -- Hallucinations -- Hallucinogen Abuse -- IACUC -- ICD -- Idiopathic -- Imaging Mass Spectrometry -- Imipramine -- Impairment of Functioning; Measurement Scales -- Kappa-Opioid Agonists -- Kappa-Receptor -- Keratotic Lesion -- Ketamine -- Ketamine -- Khat -- L-Alpha-Acetyl-Methadol -- Lamotrigine -- Lanreotide -- Latent Inhibition -- Latin Square Design -- Laxatives -- Magnetic Resonance Imaging -- Major and Minor and Mixed Anxiety-Depressive Disorders -- Major Tranquilizer -- Mania -- MAO-B Inhibitor -- Mass Spectrometry -- N-Acetylcysteine -- Nafion -- Naloxone -- Naltrexone -- N-Arachidonylethanolamine -- Narcolepsy -- ob/ob Mouse -- Obsessions -- Obsessive-Compulsive Anxiety Disorders -- Occasion Setting with Drugs -- Octreotide -- Off-Label Use of Drugs -- P300 -- Pain -- Pain and Psychopharmacology -- Pair-Feeding -- Palatability -- Paliperidone -- qEEG -- QT Interval -- Quality of Life -- Quazepam -- Quetiapine -- Radial Arm Maze -- Radionuclide -- Radiopharmaceutical -- Radiotracer -- Randomized Controlled Trials -- Rapamycin -- Saporin -- Satiety -- Saturation Binding Curve -- Scalar Property -- Schedule I -- Schedule II -- Tachykinins -- Tachyphylaxis -- Tandospirone -- Tardive Dyskinesia -- Taste Reactivity Test -- Temazepam -- Ubiquitin-Proteasome System -- Ultrasonic -- Ultrasonic Vocalizations -- Ultrasonic Vocalizations -- Unblocking -- Unconditional Response -- Vaccination -- Vaccines and Drug-Specific Antibodies -- Vagus Nerve Stimulation -- Valproic Acid -- Values-Based Medicine -- Varenicline -- Wakefulness -- Water Maze -- Whole-Cell Recording -- Wisconsin Card Sorting Test -- Wisconsin General Test Apparatus -- Withdrawal Syndromes -- Yale-Brown Obsessive-Compulsive Scale -- Yohimbine -- Zaleplon -- Zero-Order Elimination Kinetics -- Ziprasidone -- Zolpidem -- Zonisamide -- Zopiclone
    Abstract: Psychopharmacology is the study of the effects of psychoactive drugs on the functioning of the central nervous system at all levels of analysis, thus embracing cognition, behaviour, psychological states, neurophysiology, neurochemistry, gene expression and molecular biology. It includes, as an integral part of its domain, the interaction of environmental and genetic factors with psychoactive drug action, their medicinal and social uses, and their abuse. The Encyclopedia of Psychopharmacology provides detailed information on psychopharmacology and its sub-disciplines, such as clinical psychopharmacology, molecular neuropsychopharmacology, behavioural pharmacology in laboratory animals, preclinical psychopharmacology, human experimental psychopharmacology. The wide-ranging entries in the Encyclopedia of Psychopharmacology are written by leading experts. They will provide basic and clinical scientists in academia as well as industry with valuable information about the field of psychopharmacology. Also people in related fields, students, teachers, and interested laypeople will benefit from the important and relevant information on the most recent developments of psychopharmacology. Entries will fall into several main categories: Methods, techniques and target systems (explaining nature of a technique and its uses and limitations) - Drugs and classes of drug (mainly describing effects and mechanisms of action) - Psychiatric states (explaining drugs used to treat them) - Miscellaneous currently interesting topics that do not fall within the above (e.g. cognitive enhancement, regulation and licensing, substance use in religious rituals)
    Pages: XLVI, 690 p. 206 illus., 80 illus. in color. : online resource.
    ISBN: 9783642277726
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  • 51
    Keywords: Neurosciences ; Technology—Sociological aspects ; Neurosciences ; Science and Technology Studies ; Springer eBooks
    Description / Table of Contents: Introduction -- Part 1: Socializing code -- Chapter One: ́€œSharing code: social meditations in software development́€ -- Chapter Two: ́€œWriting code: software development and communicative actionś€ Part 2: Codifying society -- Chapter Three: €œÓwning code: institutional aspects of software development́€ -- Chapter Four: €œÚsing code: the social diffusion of programming taskś€ -- Conclusion
    Abstract: This book analyses the social contexts in which programmers design neuroimaging software used in brain studies. It shows that in the same way people engage in everyday communication, programmers are involved in a series of communicative processes to realize the negotiations and discussions generated by software development. In this way, highly technical activities such as computer code writing are also underpinned by values, preferences, and power relations. At the same time, the book sheds new light on scientists’ increasing dependence on software. On the one hand, many scientific tasks can no longer be performed without the help of computational technologies. On the other hand, most scientists have only superficial computing knowledge. As a result, inequalities emerge whereby some scientists take the most strategic methodological decisions whereas other scientists can only rely on the technical help provided by user-friendly computer applications
    Pages: XXIII, 426 p. 59 illus., 24 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9789811370601
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  • 52
    Keywords: Oncology ; Oncology   ; Endocrinology ; Cancer Research ; Oncology ; Endocrinology ; Springer Nature Living Reference
    Description / Table of Contents: Epidemiology: world overview, risk factors, prospects for prevention -- Development and structure of pancreas -- Pathologic classification and biological behaviour of pancreatic neoplasia -- Developmental molecular biology of the pancreas -- Molecular pathology of precursor lesions of pancreatic cancer, pancreatic intraepithelial neoplasia (PanIN), intraductal papillary mucinous neoplasm (IPMN), and mucinous cystic neoplasm (MCN) -- Epigenetics: its fundamentals and applications to a revised comprehensive progression model for pancreatic cancer -- Molecular pathology of pancreatic neuroendocrine tumours MEN-1: gastrinomas, insulinoma, Non-Functioning Tumours, VIPoma, glucagonoma, Von Hippel-Lindau, Neurofibromatosis -- Sporadic neuroendocrine pancreatic tumours -- Molecular pathology of non-pancreatic cancer lesions: Ampullary cancer, intra-pancreatic bile duct cancer, and duodenal cancer -- Miscellaneous non-pancreatic non-endocrine tumours -- Novel molecular relationships between chronic pancreatitis and cancer -- Pancreatic cancer stem cells -- Cell cycle control: signalling pathways in pancreatic pathogenesis -- Apoptosis: signalling pathways in pancreatic pathogenesis -- EGFR: signalling pathways in pancreatic pathogenesis Pl3, Akt, lKK, Ras, Raf, MAPKK, ERK -- Hedgehog: signalling pathways in pancreatic pathogenesis -- Smad4/TGF-ß pathway: signalling pathways in pancreatic pathogenesis -- Notch signalling in pancreatic morphogenesis and pancreatic cancer -- Molecular characterization of pancreatic cell lines -- Mouse models of exocrine pancreatic cancer -- Principles and applications of microarray gene expression in pancreatic cancer -- Principles and applications of proteomics in pancreatic cancer -- Tumour-stromal interaction: invasion and metastases -- Genetic susceptibility, high risk groups, chronic and hereditary pancreatitis, familial pancreatic cancer syndromes -- Inherited endocrine pancreatic tumors associated with Multiple Endocrine Neoplasia Type 1 Von-Hippel-Lindau Syndrome, and Neurofibromatosis Type 1 -- Clinical decision making in pancreatic cancer -- Paraneoplastic syndromes -- Diagnostic and therapeutic response markers -- CT and fusion PET-CT Diagnosis, staging, and follow-up -- MRI and MRCP: diagnosis and staging of pancreatic cancer -- EUS Diagnosis and staging -- Laparoscopy and laparoscopic ultrasound: diagnosis and staging -- Overview of palliative management for pancreatic cancer -- Endoscopy -- Interventional radiology -- Role of palliative surgery in advanced pancreatic cancer -- Chemotherapy for advanced pancreatic cancer -- Developments in chemoradiation in advanced pancreatic cancer -- Surgical resection for pancreatic cancer -- Role of venous resection in pancreatic cancer surgery -- Pathological reporting and staging following resection -- Japanese Pancreas Society staging for pancreatic cancer -- Adjuvant chemotherapy in pancreatic cancer -- Case for adjuvant chemoradiation therapy for pancreatic cancer -- Case for neoadjuvant treatment in pancreatic cancer -- Borderline resectable disease -- Management of cystic neoplasms serous cystic neoplasms mucinous, cystic neoplasms, intraductal paillary mucinous neoplasms -- Laparoscopic surgery for pancreatic neoplasm -- Modern Japanese approach to pancreas cancer -- Development of novel biomarkers -- Inherited genetics of pancreatic cancer and secondary screening -- Gene therapy for pancreatic cancer -- Vaccine therapy and immunotherapy -- Emerging targets in pancreatic cancer.-
    Abstract: Worldwide, there are an estimated 232, 000 new cases of pancreatic cancer annually. In the United States, it is the fourth leading cause of cancer death, and approximately 30,000 people die of pancreatic cancer each year. The disease is difficult to diagnose in its early stages, and most patients have incurable disease by the time they present with symptoms. The overall 5-year survival rate for this disease is less than 5%. In organizing this handbook, Dr. Neoptolemos and his co-editors will produce a distinguished Major Reference Work devoted to pancreatic cancer. This handbook will have widespread appeal among clinicians, pathologists and basic scientists who are now struggling to understand this complex and rapidly expanding field. Because of the recent and vast growth in both the clinical and scientific research being done in pancreatic cancer (there is currently an unprecedented investment by academia and industry in this field), each researcher’s knowledge of other specialty areas outside his or her own is now often quite limited. The aim of this book is to place these the tangible advances—those that are indispensable to all working on pancreatic cancer—readily at hand. The book will focus on advances that will not become dated, and the editors will choose authors who are the very best in each area
    Pages: 208 illus., 60 illus. in color. : online resource.
    ISBN: 9781493966318