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  • Springer  (152,914)
  • 2015-2019  (3,420)
  • 1970-1974  (115,206)
  • 1930-1934  (34,288)
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  • 1
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 2
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 3
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 4
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    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 5
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    New York : Springer
    Associated volumes
    Call number: QZ202.6:30(2)
    Keywords: AIDS malignancies ; Viral carcinogenesis ; Oncogenic Viruses ; Tumor Virus Infections ; Neoplasms / etiology ; Acquired Immunodeficiency Syndrome
    Pages: viii, 250 p. : ill. (some col.)
    Edition: 2nd ed.
    ISBN: 9783030035013
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    QZ202.6:30(2) available
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  • 6
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 7
    Call number: WN180:8
    Keywords: Diagnostic Imaging ; Artificial intelligence
    Pages: xv, 373 p. : ill.
    ISBN: 9783319948775
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    WN180:8 on loan
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  • 8
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    Cham : Springer
    Call number: QZ200:576 ; C100:50
    Pages: vii, 205 p. : ill.
    ISBN: 9783030012069
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    QZ200:576 available
    C100:50 departmental collection or stack – please contact the library
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  • 9
    Keywords: Morphology (Animals) ; Evolution (Biology) ; Paleontology  ; Animal Anatomy / Morphology / Histology ; Evolutionary Biology ; Paleontology ; Springer eBooks
    Description / Table of Contents: Evolution of Chordate Cardiopharyngeal Muscles and the Origin of Vertebrate Head Muscles -- Early Vertebrates and the Emergence of Jaws -- Cranium, Cephalic Muscles, and Homologies in Cyclostomes -- Heads, Jaws and Muscles in Chondrichthyans Actinopterygians: Head, Jaws and Muscles -- Sarcopterygian fishes, the “lobe-fins” -- Diversity of Heads, Jaws, and Cephalic Muscles in Amphibians -- Evolution, Diversity, and Development of the Craniocervical System in Turtles with Special Reference to Jaw Musculature -- An Integrative View of Lepidosaur Cranial Anatomy, Development and Diversification -- The Skull and Head Muscles of Archosauria -- The Origin and Evolution of Mammalian Head Muscles, with Special Emphasis on the Facial Myology of Primates and Modern Humans
    Abstract: The vertebrate head is the most complex part of the animal body and its diversity in nature reflects a variety of life styles, feeding modes, and ecological adaptations. This book will take you on a journey to discover the origin and diversification of the head, which evolved from a seemingly headless chordate ancestor. Despite their structural diversity, heads develop in a highly conserved fashion in embryos. Major sensory organs like the eyes, ears, nose, and brain develop in close association with surrounding tissues such as bones, cartilages, muscles, nerves, and blood vessels. Ultimately, this integrated unit of tissues gives rise to the complex functionality of the musculoskeletal system as a result of sensory and neural feedback, most notably in the use of the vertebrate jaws, a major vertebrate innovation only lacking in hagfishes and lampreys. The cranium subsequently further diversified during the major transition from fishes living in an aquatic environment to tetrapods living mostly on land. In this book, experts will join forces to integrate, for the first time, state-of-the-art knowledge on the anatomy, development, function, diversity, and evolution of the head and jaws and their muscles within all major groups of extant vertebrates. Considerations about and comparisons with fossil taxa, including emblematic groups such as the dinosaurs, are also provided in this landmark book, which will be a leading reference for many years to come
    Pages: XIV, 303 p. 95 illus., 59 illus. in color. : online resource.
    ISBN: 9783319935607
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  • 10
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 11
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    Heidelberg : Springer
    Call number: QH442:222 ; ERW
    Keywords: Genetic Techniques
    ISBN: 9783642346934
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    QH442:222 available
    ERW in acquisition
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  • 12
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 13
    Keywords: Plant breeding ; Plant genetics ; Agriculture ; Genetic Engineering ; Alternative Medicine ; Pharmaceutical technology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Agriculture ; Genetic Engineering ; Complementary & Alternative Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Foreword by Prof. M. Iqbal Choudhary, Director, International Center for Chemical and Biological Sciences -- Marine and Freshwater Organisms in Drug Discovery -- Bioactive Molecules from Malaysian Flora -- Immunomosulatory Effect of Cycloartane-Type Triterpenes -- Antigiardial Actitivty of Citrullus lanatus Extracts -- Potential Idiosyncratic Liver Toxicity of Herbal Products -- Antitrypanosomal Activity of Nigerian Plants -- Alkaloids Isolated from Malaysian Plant Families -- Pharmacological Studies on Ficus Species -- Herbals in the treatment of Salmonella typhi -- Biology of Proline-Containing Cyclic Peptides -- Plant Extracts and Memory Impairment -- Herbals Used in Heat and Detoxification in Chinese Traditional Medicine -- Secondary Metabolites from Solanaceae of Iran -- Molecular Docking Study on Rauvolfia species -- Drug Development from the farm -- Phytochemical Screening of Sri Lankan Plants -- New carbohydrate esters of cinnamic acid from the leaves of Gleditschia -- Herbals in the Treatment of Alzheimer's disease
    Abstract: Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the “scientific methods”. Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, it will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture
    Pages: XX, 385 p. 67 illus., 45 illus. in color. : online resource.
    ISBN: 9783030044084
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  • 14
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 15
    Keywords: Immunology ; Immunology ; Springer eBooks
    Description / Table of Contents: Isolation and Characterisation of Human Adipose-derived Stem Cells -- Isolation of Murine Hematopoietic Stem Cells -- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells -- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting -- Identification and isolation of Regulatory B cells in Mouse and Human -- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis -- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells -- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade -- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells -- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays -- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments -- Immune tolerance and rejection in organ transplantation -- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation -- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood -- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental -- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
    Abstract: This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 240 p. 43 illus., 36 illus. in color. : online resource.
    ISBN: 9781493989386
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  • 16
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 17
    Keywords: Emerging infectious diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Analysis of Iron Requirements and Siderophore Production -- The Pathometabolism of Legionella Studied by Isotopologue Profiling -- Quantification of Viable but Non-culturable Cells of Legionella pneumophila -- Selection of Legionella Virulence-related Traits by Environmental Protozoa -- Migration of Acanthamoeba castellanii through Legionella Biofilms -- Scar-free Genome Editing in Legionella pneumophila -- Querying Legionella Genomes using Transposition-sequencing -- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq -- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae -- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium -- Sorting of Phagocytic Cells Infected with Legionella pneumophila -- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment -- Mitochondrial Dynamics and Activity in Legionella-Infected Cells -- Perturbation of Legionella Cell Infection by RNA Interference -- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila -- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography -- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila -- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification -- Inflammasome Activation in Legionella-infected Macrophages -- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila -- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence -- Dictyostelium Host Response to Legionella Infection: Strategies and Assays -- 6.1.3 The Caenorhabditis elegans model of Legionella infection -- The Mouse as a Model for Pulmonary Legionella Infection -- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples -- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients -- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry
    Abstract: The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens
    Pages: XXVI, 476 p. 74 illus., 50 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990481
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  • 18
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels -- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling -- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators -- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes -- MCU Regulation in Lipid Bilayer and Electrophysiological Recording -- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer -- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter -- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana -- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes -- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes -- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle -- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis -- Ca2+-Dependent Regulation of TFEB and Lysosomal Function -- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum -- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2 -- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes -- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes -- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer -- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells -- Purification of Functional F-ATP Synthase from Blue Native PAGE
    Abstract: This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research
    Pages: XIII, 246 p. 48 illus., 39 illus. in color. : online resource.
    ISBN: 9781493990184
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  • 19
    Keywords: Plant breeding ; Agriculture ; Plant Breeding/Biotechnology ; Agriculture ; Springer eBooks
    Description / Table of Contents: Hybrid Sorghum Product Development and Production -- Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum -- Field Studies to Develop Weed Management Programs for Grain Sorghum -- Discovery of Sorghum Haploid Induction System -- Development of a Pedigreed Sorghum Mutant Library -- Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion -- Assaying Sorghum Nutritional Quality -- Tannin Analysis in Sorghum Grains -- Sorghum Phytochemicals and their Potential Impact on Human Health -- Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography -- Gene Editing in Sorghum through Agrobacterium -- Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment -- Novel Ternary Vectors for Efficient Sorghum Transformation -- Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa -- Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions -- Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case -- Background on Grain Sorghum Usage -- Assaying Sorghum for Fuel Production -- The Role of Sorghum in Renewables and Biofuels
    Abstract: This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum
    Pages: X, 281 p. 84 illus., 61 illus. in color. : online resource.
    ISBN: 9781493990399
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  • 20
    Keywords: Biomedical Engineering ; Biology / Data processing ; Biotechnology ; Biomedical Engineering/Biotechnology ; Computer Appl. in Life Sciences ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Enhancing Nursing Education through Affordable and Realistic Holographic Mixed Reality: The Virtual Standardized Patient for Clinical Simulation -- Potential Application Of Virtual Reality For Interface Customisation (And Pre-Training) Of Amputee Patients As Preparation For Prosthetic Use -- Visualising Medical Heritage: New Approaches To Digitisation And Interpretation Of Medical Heritage Collections -- Integrating 3D visualisation technologies in undergraduate anatomy education -- Pedagogical perspectives on the use of technology within medical curricula: moving away from norm driven implementation -- Towards a More User-Friendly Medication Information Delivery to People Living with Multiple Sclerosis: A Case Study with Alemtuzumab -- The Co-Design of Hand Rehabilitation Exercises for Multiple Sclerosis Using Hand Tracking System -- Examining Vascular Structure And Function Using Confocal Microscopy And 3D Imaging Techniques -- Which Tool Is Best: 3D Scanning Or Photogrammetry - It Depends On The Task -- Application of Photogrammetry in Biomedical Science
    Abstract: This edited volume explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in imaging and analysis, education, engagement and training. The reader will be able to explore the utilisation of technologies from a number of fields to enable an engaging and meaningful visual representation of the life sciences. This use of technology-enhanced learning will be of benefit for the learner, trainer, in patient care and the wider field of education and engagement. By examining a range of techniques in image capture (photogrammetery, stereophotogrammetry, microphotogrammetry and autostereoscopy), this book will showcase the wide range of tools we can use. Researchers in this field will be able to find something suitable to apply to their work to enhance user engagement through improved visual means using the technologies we have available to us today. It will highlight the uses of these technologies to examine many aspects of the human body, and enable improved ways to enhance visual and tactile learning, including 3D printing. By demonstrating co-design processes, working directly with the end-stage users (including patients), it will also highlight successes in adopting tools like hand motion tracking rehabilitation for patients with conditions like multiple sclerosis. The book will also discuss the applications of immersive environments including virtual, augmented and mixed reality. The ultimate aim is to show how, by using these tools, we can enhance communication, mobile applications, health literacy and illustration of both normal and pathological processes in the body. By applying a wide range of tools and technologies, this volume will highlight the wide range of applications in education, training and learning both for students and faculty, but also for patient care and education. Therefore, the work presented here can be accessed by a wide range of users from faculty and students involved in the design and development of these processes, by examining the pedagogy around these technologies. Importantly, it presents material, which will be of benefit for the patient, engaging them to become more involved with techniques like physiotherapy
    Pages: XV, 132 p. 65 illus., 42 illus. in color. : online resource.
    ISBN: 9783030060701
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  • 21
    Keywords: Human Physiology ; Human Physiology ; Springer eBooks
    Abstract: In recent years, volumes of the long-established Springer Subcellular Biochemistry series have shown great diversity of content, thus interesting biochemical topics, enthusiastically edited in all cases, have been readily accommodated. This new volume of the Subcellular Biochemistry series, Biochemistry and Cell Biology of Ageing: Part II, offers a broad overview of many different Clinical Science aspects of Ageing. The 17 chapters included in the book, contributed by knowledgeable authors, review many important topics at an advanced level. Whilst it has proved impossible to include all relevant topics, there is much present that will be of value to the reader. The book contains chapters on: “Poor Early Growth and Age-associated Disease; The Immune System and its Dysregulation with Ageing; Pulmonary Diseases and Ageing; Neurodegenerative Diseases and Ageing; Ageing and Cognition; Ageing and Osteoarthritis; Down Syndrome, Ageing and Epigenetics; The Vestibular System and Ageing; Signal Transduction, Ageing and Disease; Skin Changes During Ageing; Connective Tissue and Age-related Diseases ; Potential Cellular and Biochemical Mechanisms of Exercise and Physical Activity on the Ageing Process; Health Benefits of Anti-ageing Drugs; Visual Defects and Ageing; Hutchinson-Gilford Progeria Syndrome: Challenges at Bench and Bedside; Osteoporosis and the Ageing Skeleton; Neurovascular Ageing and Age-related Diseases” The book is primarily directed to advanced biomedical science and medical students, postgraduates, researchers, clinicians and academics in the field of Ageing. An earlier companion volume of the Subcellular Biochemistry series - Biochemistry and Cell Biology of Ageing: Part I (Volume 90) - covering the Biomedical Science aspects of ageing, has also been recently published
    Pages: X, 499 p. 53 illus., 47 illus. in color. : online resource.
    ISBN: 9789811336812
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  • 22
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Left Ventricular Strain and Relaxation are Independently Associated with Renal Cortical Perfusion in Hypertensive Patients -- Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: a Bispectral Analysis -- Influence of Heart Rate, Age, and Gender on Heart Rate Variability in Adolescents and Young Adults -- Hand-Foot Syndrome and Progression-Free Survival in Patients Treated with Sunitinib for Metastatic Clear Cell Renal Cell Carcinoma -- Elastography in the Diagnosis of Pancreatic Malignancies -- Factors Affecting Health-Related Quality of Life in Liver Transplant Patients -- Phagocytosis and Autophagy in THP-1 cells Exposed to Urban Dust: Possible Role of LC3€“Ássociated Phagocytosis and Canonical Autophagy -- Gendeŕ€“Dependent Responses of Growth and Insuliń€“like Growth Factoŕ€“1 to Growth Hormone Therapy in Prepubertal Growth Hormone Deficient Children -- Vitamin D and Calcium Homeostasis in Infants with Urolithiasis -- Morphometric Analysis of the Lumbar Vertebrae Concerning the Optimal Screw Selection for Transpedicular Stabilization -- Stress and Dehumanizing Behaviors of Medical Staff toward Patients -- Biological and Social Determinants of Maximum Oxygen Uptake in Adult Men
    Abstract: This book focuses on issues in clinical practice and research that are of general interest. The articles primarily focus on understanding the pathogenic mechanisms of diseases, their prevention, and therapy. The topics addressed include cardiovascular regulation with regard to blood pressure and heart rate variability, and to coupling blood pressure changes with subarachnoid fluid oscillations. In addition, the book discusses recent advances in the diagnostics of and targeted molecular therapy for renal and pancreatic malignancies, growth disorders, vitamin D and calcium homeostasis in children in the context of neonatal urolithiasis, and neurosurgical interventions in multifarious age-related diseases of the vertebrae. Neuropsychological aspects of patients’ quality of life and of shaping medical staff’s attitude toward patients are also addressed. The respective articles are intended to build a bridge between basic and clinical research. Further, the book enhances the current body of knowledge on diagnostics and patient treatment and offers valuable new perspectives on practical clinical issues. As such, it offers a unique resource for clinicians, family physicians, medical scholars, and professionals engaged in patient management
    Pages: VI, 114 p. 20 illus. in color. : online resource.
    ISBN: 9783030129231
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  • 23
    Keywords: Otorhinolaryngology ; Neurosciences ; Otorhinolaryngology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Visual Influence on Auditory Perception -- Cue Combination Within a Bayesian Framework -- Toward a Model of Auditory-Visual Speech Intelligibility -- An Object-Based Interpretation of Audiovisual Processing -- Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway -- Multisensory Processing in the Auditory Cortex -- Audiovisual Integration in the Primate Prefrontal Cortex -- Using Multisensory Integration to Understand the Human Auditory Cortex -- Combining Voice and Face Content in the Primate Temporal Lobe -- Neural Network Dynamics and Audiovisual Integration -- Cross-Modal Learning in the Auditory System -- Multisensory Processing Differences in Individuals with Autism Spectrum Disorder
    Abstract: Auditory behavior, perception, and cognition are all shaped by information from other sensory systems. This volume examines this multi-sensory view of auditory function at levels of analysis ranging from the single neuron to neuroimaging in human clinical populations. Visual Influence on Auditory Perception Adrian K.C. Lee and Mark T. Wallace Cue Combination within a Bayesian Framework David Alais and David Burr Toward a Model of Auditory-Visual Speech Intelligibility Ken W. Grant and Joshua G. W. Bernstein An Object-based Interpretation of Audiovisual Processing Adrian K.C. Lee, Ross K. Maddox, and Jennifer K. Bizley Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway Shawn M. Willett, Jennifer M. Groh, Ross K. Maddox Multisensory Processing in the Auditory Cortex Andrew J. King, Amy Hammond-Kenny, Fernando R. Nodal Audiovisual Integration in the Primate Prefrontal Cortex Bethany Plakke and Lizabeth M. Romanski Using Multisensory Integration to Understand Human Auditory Cortex Michael S. Beauchamp Combining Voice and Face Content in the Primate Temporal Lobe Catherine Perrodin and Christopher I. Petkov Neural Network Dynamics and Audiovisual Integration Julian Keil and Daniel Senkowski Cross-Modal Learning in the Auditory System Patrick Bruns and Brigitte Röder Multisensory Processing Differences in Individuals with Autism Spectrum Disorder Sarah H. Baum Miller, Mark T. Wallace Adrian K.C. Lee is Associate Professor in the Department of Speech & Hearing Sciences and the Institute for Learning and Brain Sciences at the University of Washington, Seattle Mark T. Wallace is the Louise B McGavock Endowed Chair and Professor in the Departments of Hearing and Speech Sciences, Psychiatry, Psychology and Director of the Vanderbilt Brain Institute at Vanderbilt University, Nashville Allison B. Coffin is Associate Professor in the Department of Integrative Physiology and Neuroscience at Washington State University, Vancouver, WA Arthur N. Popper is Professor Emeritus and research professor in the Department of Biology at the University of Maryland, College Park Richard R. Fay is Distinguished Research Professor of Psychology at Loyola University, Chicago
    Pages: XVI, 272 p. 70 illus., 49 illus. in color. : online resource.
    ISBN: 9783030104610
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  • 24
    Keywords: Biochemistry ; Neurosciences ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease
    Abstract: This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects
    Pages: XII, 289 p. 66 illus., 55 illus. in color. : online resource.
    ISBN: 9781493991242
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  • 25
    Keywords: Cell Culture ; Laboratory animals ; Cell Culture ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Applicability of Mouse Models to the Study of Human Disease -- Optimizing the Cell Culture Microenvironment -- Propagation and Maintenance of Mouse Embryonic Stem Cells -- Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells -- Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction -- Gene Editing of Mouse Embryonic and Epiblast Stem Cells -- Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood -- Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture -- Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation -- Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo -- Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells -- Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells -- Organotypic Culture of Adult Mouse Retina -- Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes -- Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes -- Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells -- An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells -- Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans -- Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers -- The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential -- Mouse Lung Tissue Slice Culture
    Abstract: This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets
    Pages: XII, 315 p. 64 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990863
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  • 26
    Keywords: Biotechnology ; Biochemistry ; Biotechnology ; Protein Science ; Springer eBooks
    Description / Table of Contents: Established and Upcoming Yeast Expression Systems -- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts -- Pichia pastoris Promoters -- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes -- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha -- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica -- High-throughput Screening and Selection of Pichia pastoris Strains -- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke -- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori -- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae -- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast -- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production -- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments -- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris -- Efficient Development of a Mixed Feed Process for Pichia pastoris -- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification -- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants -- LC-MS analysis of (glyco)-proteins of Pichia pastoris -- Assessing the Quality of Recombinant Products Made in Yeast -- Quantification of Recombinant Products In Yeast
    Abstract: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications
    Pages: XII, 431 p. 60 illus., 46 illus. in color. : online resource.
    ISBN: 9781493990245
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  • 27
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    New York : Springer
    Keywords: Medicine ; Orthopedics ; Molecular Medicine ; Orthopedics ; Springer eBooks
    Description / Table of Contents: Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone
    Abstract: This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research
    Pages: XV, 670 p. 172 illus., 121 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493989973
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  • 28
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 29
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 30
    Keywords: Medicine ; Dentistry ; Molecular Medicine ; Dentistry ; Springer eBooks
    Description / Table of Contents: Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors -- Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line -- Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells -- Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors -- Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis -- Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions -- Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering -- Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry -- Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering -- In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy -- A Mouse Model to Study Reparative Dentinogenesis -- Multiwalled Carbon Nanotubes for Dental Applications -- Peptide-mediated Biomimetic Regrowth of Human Enamel in situ -- Bioengineering Tooth Bud Constructs using GelMA Hydrogel -- Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes -- In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes -- Methods for in situ Proteins Visualization in Dental Mineralized Tissues -- In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection -- Immunofluorescence Procedure for Developing Enamel Tissues -- Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos -- Isolation of SIBLING Proteins from Bone and Dentin Matrix -- Immunohistochemical Co-localization of Amelogenin and Ameloblastin in Developing Enamel Matrix -- The Expression and Purification of Recombinant Mouse Ameloblastin in e-coli -- Protocols for Studying Formation and Mineralization of Dental Tissues in vivo -- Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth -- Purification of Developing Enamel Matrix Proteins using Preparative SDS PAGE -- Using ImageJ (FIJI) to Analyse and Present X Ray CT Images of Enamel -- Scanning Electron Microscopy (SEM) Methods for Dental Enamel -- Microcomputed Tomography Imaging in Odontogenesis Studies -- Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues -- Rodent Dental Fluorosis Model--Extraction of Enamel Organ from Rat Incisors -- Three Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-beam Computed Tomography (CBCT) -- Protocols to Study Dental Caries In Vitro: Microbial Caries Models -- In vitro Caries Models for the Assessment of Novel Restorative Materials -- Protocols to Study Dental Caries In Vitro: pH Cycling Models -- In Vivo Rodent Models for Studying Dental Caries and Pulp Disease -- Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement -- Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues -- Protocols, Methods, and Tools for Genome-wide Association Studies (GWAS) of Dental Traits -- Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition -- The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Oral Metagenomics, Metatranscriptomics and Metabolomics Studies of the Oral Microbiome -- Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarkers Screening
    Abstract: This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth
    Pages: XVIII, 565 p. 152 illus., 135 illus. in color. : online resource.
    ISBN: 9781493990122
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  • 31
    Keywords: Pharmaceutical technology ; Bioinformatics ; Pharmaceutical Sciences/Technology ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Miniaturized Checkerboard Assays to Measure Antibiotic Interactions -- High-Throughput Screening for Drug Combinations -- Post-Processing of Large Bioactivity Data -- How to Develop a Drug Target Ontology: KNowledge Acquisition and Representation Methodology (KNARM) -- A Guide to Dictionary-Based Text Mining -- Leveraging Big Data to Transform Drug Discovery -- How to Prepare a Compound Collection Prior to Virtual Screening -- Building a Quantitative Structure-Property Relationship (QSPR) Model -- Isomeric and Conformational Analysis of Small Drug and Drug-Like Molecules by Ion Mobility Mass Spectrometry (IM-MS) -- A Computational Platform and Guide for Acceleration of Novel Medicines and Personalized Medicine -- Omics Data Integration and Analysis for Systems Pharmacology -- Bioinformatics Based Tools and Software in Clinical Research: A New Emerging Area -- Text Mining for Drug Discovery -- Big Data Cohort Extraction for Personalized Statin Treatment and Machine Learning -- Drug Signature Detection Based on L1000 Genomic and Proteomic Big Data -- Drug Effect Prediction by Integrating L1000 Genomic and Proteomic Big Data -- A Bayesian Network Approach to Disease Subtype Discovery
    Abstract: This third edition volume expands on the previous editions with new topics that cover drug discovery through translational bioinformatics, informatics, clinical research informatics, as well as clinical informatics. The chapters discuss new methods to study target identification, genome analysis, cheminformatics, protein analysis, and text mining. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, software workflows, reagents and on-line resources, together with step-by-step, readily reproducible laboratory and computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Bioinformatics and Drug Discovery, Third Edition is a valuable resource for anyone interested in drug design, including academicians (biologists, informaticists and data scientists, chemists, and biochemists), clinicians, and pharmaceutical scientists
    Pages: XI, 324 p. 63 illus., 54 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493990894
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  • 32
    Keywords: Immunology ; Laboratory animals ; Immunology ; Animal Models ; Springer eBooks
    Description / Table of Contents: CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells -- Genome Editing in Mouse Embryos with CRISPR/Cas9 -- Derivation of Macrophages from Mouse Bone Marrow -- Bone Marrow Derived Dendritic Cells -- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils -- In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets -- Generation and Culture of Mouse Embryonic Fibroblasts -- Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation -- Depletion and Reconstitution of Macrophages in Mice -- Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making -- A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colz -- Mouse Model of Staphylococcus aureus Skin Infection -- Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses -- Endotoxin-Induced Uveitis in Rodents -- Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice -- Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages -- Influenza-Mediated Lung Infection Models -- Adoptive Transfer Colitis -- The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC) -- Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA) -- Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions -- Sepsis Induced by Cecal Ligation and Puncture
    Abstract: This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system
    Pages: XI, 258 p. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991679
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  • 33
    Keywords: Medicine ; Neurology ; Human Genetics ; Molecular Medicine ; Neurology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Fragile-X Syndrome: Introduction -- Clinical Genetic Testing for Fragile-X Syndrome by Polymerase Chain Reaction Amplification and Southern Blot Analyses -- Monitoring for Epigenetic Modifications at the FMR1 Locus -- Assays for Determining Repeat Number, Methylation Status and AGG Interruptions in the Fragile X-Related Disorders -- One-Step Generation of Seamless Luciferase Gene Knock-In Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells -- Modeling FXS with Mouse Neural Progenitors -- Using Human Neural Progenitor Cell Models to Conduct Large-Scale Drug Screens for Neurological and Psychiatric Diseases -- Modeling FXS: Human Pluripotent Stem Cells and In Vitro Neural Differentiation -- Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders -- Imaging of Somatic Ca2+ Transients in Differentiated Human Neurons -- Patch-Clamp Recordings from Human Embryonic Stem Cells-Derived Fragile X Neurons -- Application of Drosophila Model towards Understanding the Molecular Basis of Fragile-X Syndrome -- Comparing Mouse and Human-Based Models for Fragile-X Syndrome Pre-Clinical Research -- Pathophysiology Mechanisms in Fragile-X Primary Ovarian Insufficiency -- Fragile-X Associated Tremor/Ataxia Syndrome (FXTAS)
    Abstract: This volume discusses the latest technologies used to study all aspects of Fragile-X Syndrome (FXS). The chapters in this book cover topics such as monitoring for epigenetic modifications at the FMR1 locus; modeling FXS with human pluripotent stem cells, mouse neural progenitors; mouse versus human-based models for FXS pre-clinical research; and Fragile-X associated with Tremor/Ataxia Syndrome (FXTAS). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Fragile-X Syndrome: Methods and Protocols is a valuable tool to help scientists working towards one day developing a therapeutic solution to improve the symptoms of FXS. Chapter "Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
    Pages: X, 192 p. 25 illus., 15 illus. in color. : online resource.
    ISBN: 9781493990801
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  • 34
    Keywords: Bioinformatics ; Biomedical Engineering ; Bioinformatics ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: A Pycellerator Tutorial -- Using RuleBuilder to Graphically Define and Visualize BioNetGen-Language Patterns and Reaction Rules -- Strategy-Driven Exploration for Rule-Based Models of Biochemical Systems with PORGY -- Using rxncon to Develop Rule-Based Models -- Efficiently Encoding Complex Biochemical Models with the Multistate Model Builder (MSMB) -- Multi-Level Modeling and Simulation of Cellular Systems: An Introduction to ML-Rules -- Using Python for Spatially Resolved Modeling with Simmune -- Rule-Based Modeling using Wildcards in the Smoldyn Simulator -- MCell-R: A Particle-Resolution Network-Free Spatial Modeling Framework -- Spatial Rule-Based Simulations: the SRSim Software -- CellOrganizer: Learning and Using Cell Geometries for Spatial Cell Simulations -- Using Mechanistic Models for Analysis of Proteomic Data -- Annotations for Rule-Based Models -- Markov Chain Aggregation and Its Application to Rule-Based Modelling -- In Silico Evolution of Signaling Networks Using Rule-Based Models: Bistable Response Dynamics -- Recipes for Analysis of Molecular Networks Using the Data2Dynamics Modeling Environment -- RKappa: Software for Analyzing Rule-Based Models -- A Step-by-Step Guide to Using BioNetFit
    Abstract: This volume covers a variety of topics related to the practice of rule-based modeling, a type of mathematical modeling useful for studying biomolecular site dynamics. There is an emphasis on software tools and detailed descriptions of techniques. The chapters in this book discuss topics such as software tools and frameworks for compartmental modeling (Pycellerator, RuleBuilder, Prgy, rxncon, MSMB, and ML-Rules); tools for spatial modeling (Simmune, Smoldyn, MCell-R, SRSim, and CellOrganizer); rule-based models to analyze proteomic data; model annotation; Markov chain aggregation; BioJazz; and methods to identify model parameters (Data2Dynamics, RKappa, and BioNetFit). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary resources, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Biomolecular Site Dynamics: Methods and Protocols is a valuable resource for both the novice and expert rule-based modeler. It will also appeal to systems biologists and help them enhance their studies with easy-to-read and write rule-based models
    Pages: XIX, 423 p. 188 illus., 108 illus. in color. : online resource.
    ISBN: 9781493991020
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    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK! -- Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis -- Identification of Long Non-Coding RNAs in the Developing Endosperm of Maize -- RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize -- Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues -- Medium Throughput RNA In Situ Hybridization of Serial Sections from Paraffin-Embedded Tissue Microarrays -- Purification and Functional Analysis of Plant Long Non-Coding RNAs (lncRNA) -- The Involvement of Long Non-Coding RNAs in Response to Plant Stress -- Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance -- Discovery, Identification, and Functional Characterization of Plant Long Intergenic Non-Coding RNAs after Virus Infection -- Bioinformatics Approaches to Studying Plant Long Non-Coding RNAs (lncRNAs): Identification and Functional Interpretation of lncRNAs from RNA-seq Data Sets -- Identification of Novel lincRNA and Co-Expression Network Analysis using RNA Sequencing Data in Plants -- An Easy-to-Follow Pipeline for Long Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria vesca -- Reference-Based Identification of Long Non-Coding RNAs in Plants with Strand-Specific RNA-Sequencing Data -- NAMS: Non-Coding Assessment in Magnoliophyta Species -- De novo Plant Transcriptome Assembly and Annotation using Illumina RNA-seq Reads -- Identification of Long Non-Coding RNA-Protein Interactions through In Vitro RNA Pull-Down Assay with Plant Nuclear Extracts -- Analysis of Interaction between Long Non-Coding RNAs and Protein by RNA-Immunoprecipitation in Arabidopsis -- Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants -- In Vivo Genome-Wide RNA Structure Probing with Structure-seq2 -- Using Protein Interaction Profile Sequencing (PIP-seq) to Identify RNA Secondary Structure and RNA-Protein Interaction Sites of Long Non-Coding RNAs in Plants -- Computationally Characterizing Protein Bound Long Non-Coding RNAs and their Secondary Structure using Protein Interaction Profile Sequencing (PIP-seq) in Plants -- Stalking Structure in Plant Long Non-Coding RNAs -- Transcriptome-Wide Mapping 5-methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant -- A Walkthrough to the Use of GreeNC: The Plant lncRNA Database -- CANTATAdb 2.0: Expanding the Collection of Plant Long Non-Coding RNAs -- Experimentally Validated Plant lncRNAs in EVLncRNAs Database -- In Situ Hi-C for Plants: An Improved Method to Detect Long-Range Chromatin Interactions
    Abstract: This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology
    Pages: XVII, 480 p. 78 illus., 67 illus. in color. : online resource.
    ISBN: 9781493990450
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    facet.materialart.
    New York, NY : Springer
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: Nonsurgical Hammertoe Treatment Options -- PIPJ Arthroplasty without Fixation -- PIPJ Interpositional Arthroplasty -- PIPJ Arthroplasty with Temporary Kirchner Wire Fixation -- PIPJ Arthrodesis, Peg and Hole -- PIPJ Arthrodesis via Buried Kirchner Wire -- PIPJ Arthrodesis with a 1 Component Metallic Implant -- PIPJ Arthrodesis with a 2 Component Metallic Implant -- PIPJ Arthrodesis with a Thermal Activated Metallic Implant -- PIPJ Arthrodesis with an Allograft Implant -- PIPJ Arthrodesis with a Bioabsorbable Implant -- Flexor Tendon Transfer -- Tenotomies -- MTP Joint: Soft Tissue Balancing (skin plasties and lengthenings, MTPJ release, extensor tendon lengthening, capsulotomy, capsulorraphy) -- MTP Joint: Plantar Plate Reconstruction via a Dorsal Approach -- MTP Joint: Plantar Plate Repair via a Plantar Approach -- Lesser Metatarsal Osteotomies: uniplanar, biplanar wedge osteotomies -- MTP Joint: Transverse Plane Correction via Collateral Ligament Repair -- MTP Joint Transverse Plane Correction via Metatarsal Osteotomy -- Crossover Toe Correction -- Congenital Overlapping Fifth Toe -- Flail Hammertoe Correction
    Abstract: Comprised exclusively of clinical cases demonstrating the various management strategies for hammertoes, this concise, practical casebook will provide foot and ankle surgeons with the best real-world strategies to properly treat these frequent deformities of the lower extremity and their complications. Beginning with a review of the relevant anatomy and biomechanics of the toe, each subsequent chapter includes case material with a unique clinical presentation, followed by a description of the diagnosis, assessment and management techniques used to treat it, as well as the case outcome, literature review and clinical pearls and pitfalls. Chapters included illustrate different surgical fixation techniques, including PIPJ arthroplasty and arthrodesis, tendon transfers and tenotomies, and osteotomies, as well as management strategies for complications. Pragmatic and reader-friendly, Hammertoes: A Case-Based Approach is an excellent resource for foot and ankle surgeons treating this common but frustrating condition
    Pages: XIII, 419 p. 511 illus., 427 illus. in color. : online resource.
    ISBN: 9783319165523
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    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media -- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell -- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell -- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome -- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host -- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome -- Gene Expression Analysis in the Pneumococcus -- Transcriptional Knockdown in Pneumococci using CRISPR Interference -- Protein Expression Analysis by Western Blot and Protein-Protein Interactions -- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony -- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria -- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells -- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae -- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease -- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection -- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo -- The Pneumococcus and Its Critical Role in Public Health -- The Epidemiology and Biostatistics of Pneumococcus
    Abstract: This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field
    Pages: XV, 227 p. 37 illus., 26 illus. in color. : online resource.
    ISBN: 9781493991990
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    Keywords: Biochemistry ; Bioinformatics ; Protein Structure ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 55 Years of the Rossmann Fold -- Advances in Protein Super-Secondary Structure Prediction and Application to Protein Structure Prediction -- Automated Family-Wide Annotation of Secondary Structure Elements -- Computational Prediction of Secondary and Supersecondary Structures from Protein Sequences -- StackSSSPred: A Stacking Based Prediction of Supersecondary Structure from Sequence -- Information-Theoretic Inference of an Optimal Dictionary of Protein Supersecondary Structures -- Secondary and Supersecondary Structure of Proteins as Coupling between Local and Local and Backbone-Electrostatic Interactions: A View through Cluster-Cumulant Scope -- Learning Organizations of Protein Energy Landscapes: An Application on Decoy Selection in Template-Free Protein Structure Prediction -- Exploring Protein Supersecondary Structure through Changes in Protein Folding, Stability, and Flexibility -- Protodomains: Symmetry Related Supersecondary Structures in Proteins and Self-Complementarity -- bab Super Secondary Motifs: Sequence, Structural Overview and Pursuit of Potential Autonomously Folding bab Sequences from (b/a)8/TIM Barrels -- Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for their Prediction and Characterization -- Stable Sub-Structures in Proteins and How to Find Them using Single-Molecule Force Spectroscopy -- Supersecondary Structures and Fragment Libraries -- Molecular Dynamics Simulations of Conformational Conversions in Transformer Proteins -- Sequence Pattern for Supersecondary Structure of Sandwich-Like Proteins -- Homology Searches using Supersecondary Structure Code -- Beyond Supersecondary Structure: Physics-Based Sequence Alignment -- Secondary and Supersecondary Structure of Proteins in Light of the Structure of Hydrophobic Cores -- Hands On: Using Tryptophan Fluorescence Spectroscopy to Study Protein Structure -- Structural Characterization of Membrane Protein Dimers
    Abstract: This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation
    Pages: XVIII, 438 p. 119 illus., 103 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991617
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  • 39
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions -- Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c -- Value of DSC in Characterization and Optimization of Protein Stability -- Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC) -- Intrinsic Thermodynamics of Protein – Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design -- Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions -- ITC Studies of Ribosome/Antibiotics Interactions -- High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry -- ITC Measurement for High Affinity Aptamers Binding to their Target Proteins -- Thermodynamics of Molecular Machines using Incremental ITC -- Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry -- Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC) -- Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC -- Tinkering with Binding Polynomials in Isothermal Titration Calorimetry -- Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine -- Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter -- Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry -- Characterization of Enzymatic Reactions using ITC
    Abstract: This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 268 p. 60 illus., 40 illus. in color. : online resource.
    ISBN: 9781493991792
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    Keywords: Biochemistry ; Cytology ; Cell receptors ; Protein Science ; Cell Biology ; Receptors ; Springer eBooks
    Description / Table of Contents: A Brief History of the b-Arrestins -- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling -- Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET -- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle -- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer -- Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET -- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin -- Methods to Investigate Arrestins in Complex with Phosphodiesterases -- Methods to Characterize Protein Interactions with β-arrestin in cellulo -- Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling -- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing -- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners -- Workflow Description to Dynamically Model β-Arrestin Signaling Networks -- Proteomic Analysis of the β-Arrestin Interactome -- Quantitating Ligand Bias using the Competitive Model of Ligand Activity -- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins -- Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy -- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins -- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors -- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling -- Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease -- Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function -- Methods to Investigate β-Arrestin Function in Metabolic Regulation -- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease -- Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells
    Abstract: This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins
    Pages: XIV, 409 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493991587
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    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 42
    Keywords: Bioinformatics ; Medical genetics ; Bioinformatics ; Gene Function ; Springer eBooks
    Description / Table of Contents: tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences -- Predicting RNA Families In Nucleotides Sequences Using StructRNAfinder -- Structural and Functional Annotation of Eukaryotic Genomes with GenSAS -- Practical Guide for Fungal Gene Prediction from Genome Assembly and RNA-seq Reads by FunGAP -- Whole-Genome Annotation with BRAKER -- EuGene: An Automated Integrative Gene Finder for Eukaryotes and Prokaryotes -- ChemGenome2.1: An ab initio Gene Prediction Software -- Multi-Genome Annotation with AUGUSTUS -- GeMoMa: Homology-Based Gene Prediction Utilizing Intron Position Conservation and RNA-seq Data -- Coding Exon-Structure Aware Realigner (CESAR): Utilizing Genome Alignments for Comparative Gene Annotation -- Predicting Genes in Closely Related Species with Scipio and WebScipio -- AnABlast, Re-Searching for Protein-Coding Sequences in Genomic Regions -- Generating Publication-Ready Prokaryotic Genome Annotations with DFAST -- BUSCO: Assessing Genome Assembly and Annotation Completeness -- Evaluating Genome Assemblies and Gene Models using gVolante -- Choosing the Best Gene Predictions with GeneValidator -- COGNATE: Comparative Gene Annotation Characterizer
    Abstract: This volume introduces software used for gene prediction with focus on eukaryotic genomes. The chapters in this book describe software and web server usage as applied in common use-cases, and explain ways to simplify re-annotation of long available genome assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary computational requirements, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Gene Prediction: Methods and Protocols is a valuable resource for researchers and research groups working on the assembly and annotation of single species or small groups of species. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com
    Pages: XI, 284 p. 54 illus., 49 illus. in color. : online resource.
    ISBN: 9781493991730
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    facet.materialart.
    New York, NY : Springer
    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp
    Abstract: This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide
    Pages: XIII, 370 p. 77 illus., 60 illus. in color. : online resource.
    ISBN: 9781493992102
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  • 44
    Keywords: Medicine ; Pharmaceutical technology ; Cardiovascular System ; Molecular Medicine ; Pharmaceutical Sciences/Technology ; Cardiovascular Biology ; Springer eBooks
    Description / Table of Contents: Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview -- Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry -- Analysis of Vascular Hydrogen Sulfide Biosynthesis -- Measurement of Protein Persulfidation: Improved Tag-Switch Method -- ProPerDP: A Protein Persulfide Detection Protocol -- Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells -- In Vitro Measurement of H2S-Mediated Vasoactive Responses -- In Vivo Measurement of H2S, Polysulfides, and ‘SSNO-mix’-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform -- Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries -- The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum -- Pharmacological Tools for the Study of H2S Contribution to Angiogenesis -- Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats -- Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats -- Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity -- Vascular Myography to Examine Functional Responses of Isolated Blood Vessels
    Abstract: This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study
    Pages: X, 221 p. 70 illus., 47 illus. in color. : online resource.
    ISBN: 9781493995288
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  • 45
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Advancements in Managing Intracerebral Hemorrhage: Transition from Nihilism to Optimism -- Mandibular Advancement Devices in Patients with Symptoms of Obstructive Sleep Apnea: A Review -- Online Health Technologies and Mobile Devices: Attitudes, Needs and Future -- Radiological Response and Neutrophil-to-Lymphocyte Ratio as Predictive Factors for Progression-Free and Overall Survival in Metastatic Renal Cell Carcinoma Patients Treated with Sunitinib -- Plasminogen Activator Inhibitor Type 1 in Blood at Onset of Chemotherapy Unfavorably Affects Survival in Primary Ovarian Cancer -- Sialidase Attenuates Epidermal Growth Factor Response and Abolishes Antiproliferative Effects of Erlotinib in A549Alveolar Epithelial Cells -- Systemic Sclerosis and Serum Content of Transforming Growth Factor -- Protein-Bound Solute Clearance during Hemodialysis -- Mold Sensitization in Asthmatic and Non-Asthmatic Subjects Diagnosed with Extract-Based versus Component-Based Allergens -- Fructose Consumption and Lipid Metabolism in Obese Children and Adolescents -- Vaccination against Measles, Mumps, and Rubella in the Light of Current Epidemic Threats: Unjustified Postponement -- Variability of Dry-Eye Disease Following Removal of Lacrimal Glands in Rats
    Abstract: This book shares the latest research and practice-oriented findings in medical sciences with a wide audience. It addresses a range of contemporary issues, often unresolved or contentious, across various medical fields, including advances in the management of hemorrhagic brain stroke. It also discusses metastatic renal cell carcinoma – a global scourge with an extremely poor long-term survival prognosis, the course and sequelae of renal cell carcinoma, as well as advances in targeted molecular therapy with sunitinib, a receptor tyrosine kinase inhibitor. Further, it examines the molecular targeting of proliferative signaling of the epidermal growth factor receptor in the first-line treatment of patients with metastatic non-small-cell lung cancer. Other articles cover clearance of toxins in hemodialyzed patients; the search for diagnostic and therapeutic markers in the connective tissue disease scleroderma; obesity linked to inappropriate dietary habit; clinical problems related to the diagnosis of sensitization to fungi and its role in asthma; and reasons for the perilous trend of avoiding basic vaccinations in children. Lastly, the book explores the rapid developments in e-health technologies that increase access to health services, particularly for the elderly. The book is intended for clinical specialists, researchers, and all allied health professionals from various fields
    Pages: VI, 115 p. 17 illus., 10 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030190590
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  • 46
    Keywords: Botany ; Biochemistry ; Plant physiology ; Pharmacy ; Plant Sciences ; Biochemistry, general ; Plant physiology ; Pharmacy ; Springer eBooks
    Abstract: Presents the latest R & D information on medicinal mushrooms from diverse geographical locations Offers comprehensive coverage of the most important application areas of medicinal mushrooms Includes contributions by eminently experienced researchers in the field of medicinal mushrooms Medicinal mushrooms are increasingly gaining attention worldwide because of their pharmacologically bioactive compounds, which have demonstrated potent and unique clinical properties. Scientific studies carried out during the last decade have confirmed their efficacy in treating a wide range of diseases. Extracts and bioactive compounds obtained from mushrooms have been used medicinally as anticancer, immunomodulator, antibacterial, antiviral, anti-inflammatory, anti-atherosclerotic, neuroprotectant, cardioprotectant, antioxidant, and anti-hypoglycemic agents, and in stem cell-based therapies. Introducing readers to the latest developments in, and ongoing research efforts on, medicinal mushrooms, this book gathers articles contributed by eminent researchers in different disciplines and from around the globe. Highlighting the tremendous potential of mushrooms for the development of new drugs, the topics covered include but are not limited to: Recent progress in research on the pharmaceutical potential of medicinal mushrooms and prospects for their clinical application. Edible mushroom neuronutraceuticals: Basis of therapeutics Overview of therapeutic efficacy of mushrooms Mushrooms – a wealth of resources for prospective stem cell-based therapies Mushrooms as potential natural cytostatics
    Pages: XIX, 419 p. 82 illus., 46 illus. in color. : online resource.
    ISBN: 9789811363825
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  • 47
    Keywords: Microbiology ; Emerging infectious diseases ; Gastroenterology ; Medical Microbiology ; Infectious Diseases ; Microbiology ; Gastroenterology ; Springer eBooks
    Description / Table of Contents: From the contents: 1. The newborn -- Microbiota development of newborns starts in utero -- Pathogenesis and possible prevention of Necrotizing Enterocolitis -- Can post-biotics represent a new strategy for NEC? - 2. The Infant -- Preventing and treating colics -- Food Allergy prevention -- Can celiac disease be prevented with probiotics? 3. The child -- Fighting fatty liver with probiotics -- Inflammatory Bowel Disease -- Acute Infectious Diarrhea -- Functional GI disorders -- Clostridium difficile colitis prevention and treatment
    Abstract: This book shows the huge impact the gut microbiota has on the gastrointestinal health of humans with a particular focus on children. It also highlights the potential use of probiotic microorganisms to protect or improve children’s gastrointestinal health. Humans are not single organisms: We are a multi-organism structure composed of ourselves and our microbiota, living in close symbiosis since birth and even before. The huge impact that the billons of microscopic cells living in our gut have on our gastrointestinal and systemic health cannot be overestimated. The enormous progress that has been made in the past decade in our still very incomplete understanding of the gut microbiota is opening the door to potential applications in human health that were simply unthinkable before. One of the most interesting aspects of this new scientific horizon is the fact that we may identify (or even create in the laboratory) and utilize many of these “friendly bacteria” to protect, or improve our health. Thus, strains of probiotic microorganisms are being identified and studied in a vast array of clinical scenarios. Among the most investigated areas for probiotics is the gastrointestinal health of children. The topics addressed in this book are spanning from the development of the gut microbiota in the fetus and newborn all the way to current and potential applications in disparate conditions such as necrotizing enterocolitis, or infectious, or inflammatory conditions affecting the child. The book is written in a rigorous, evidence based manner by an international group of outstanding experts in these fields and is aimed at pediatric gastroenterologists, pediatricians and physician scientists alike
    Pages: VIII, 150 p. 9 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030146368
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  • 48
    Keywords: Oncology ; Human Genetics ; Database management ; Data mining ; Cancer Research ; Human Genetics ; Database management ; Data Mining and Knowledge Discovery ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction -- Resources -- Data Retrieval -- Text Processing -- Semantic processing -- Index
    Abstract: This open access book is a step-by-step introduction on how shell scripting can help solve many of the data processing tasks that Health and Life specialists face everyday with minimal software dependencies. The examples presented in the book show how simple command line tools can be used and combined to retrieve data and text from web resources, to filter and mine literature, and to explore the semantics encoded in biomedical ontologies. To store data this book relies on open standard text file formats, such as TSV, CSV, XML, and OWL, that can be open by any text editor or spreadsheet application. The first two chapters, Introduction and Resources, provide a brief introduction to the shell scripting and describe popular data resources in Health and Life Sciences. The third chapter, Data Retrieval, starts by introducing a common data processing task that involves multiple data resources. Then, this chapter explains how to automate each step of that task by introducing the required commands line tools one by one. The fourth chapter, Text Processing, shows how to filter and analyze text by using simple string matching techniques and regular expressions. The last chapter, Semantic Processing, shows how XPath queries and shell scripting is able to process complex data, such as the graphs used to specify ontologies. Besides being almost immutable for more than four decades and being available in most of our personal computers, shell scripting is relatively easy to learn by Health and Life specialists as a sequence of independent commands. Comprehending them is like conducting a new laboratory protocol by testing and understanding its procedural steps and variables, and combining their intermediate results. Thus, this book is particularly relevant to Health and Life specialists or students that want to easily learn how to process data and text, and which in return may facilitate and inspire them to acquire deeper bioinformatics skills in the future
    Pages: XV, 98 p. 483 illus., 74 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030138455
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  • 49
    Keywords: Medicine ; Biochemistry ; RNA-ligand interactions ; Pharmaceutical technology ; Molecular Medicine ; Medical Biochemistry ; Protein-Ligand Interactions ; Medicinal Chemistry ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Final ToC tbd. 1) Description of human carbonic anhydrases (12 catalytically active human isoforms; isoforms as drug targets, enzymatic activity, catalytic mechanism, thermodynamics of protonation of the water molecule in the active site) -- 2) Thermal stabilities of all isoform CA catalytic domains -- Thermodynamics of inhibitor binding to CAs (incl. intrinsic thermodynamics - not mentioned important subject in most CA literature) -- 3) Comparison of methods to determine inhibitor binding to CAs (ITC, FTSA, enzymatic activity assays) -- 4) Chemical synthesis of CA inhibitors -- 5) X-ray crystallographic structures of CAs and their complexes with inhibitors -- 6) Antibodies against CAs
    Abstract: This book offers deep insights into the thermodynamics and molecular structures of the twelve catalytically active isoforms of human carbonic anhydrase (CA) with a particular focus on inhibitor binding for drug design. X-ray crystallographic structures in combination with enzyme kinetic testing provide information on the interaction of CAs and their inhibitors, knowledge which is crucial for rational drug design. CAs are zinc carrying enzymes that catalyse the reversible interconversion of carbon dioxide and bicarbonate and are involved in numerous cellular processes. They are therefore a common target for drugs. The suppression of CA activities through inhibitory compounds has found application for example in diuretics and in glaucoma therapy. In this book methods used to determine binding thermodynamics of inhibitory compounds (Isothermal titration calorimetry, Fluorescent thermal shift assay/differential scanning fluorimetry and others) will be compared in detail. Also types and chemical synthesis of CA inhibitors, the use of antibodies against CAs as well as inhibitor application in animals are discussed
    Pages: XXVI, 353 p. 169 illus., 107 illus. in color. : online resource.
    ISBN: 9783030127800
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  • 50
    Keywords: Biomedical Engineering ; Oncology ; Biomedical Engineering/Biotechnology ; Mathematical Modeling and Industrial Mathematics ; Cancer Research ; Biomedical Engineering and Bioengineering ; Springer eBooks
    Description / Table of Contents: Fixed Boundary PDE Model Formulation -- Fixed Boundary PDE Model Implementation -- Fixed Boundary PDE Model Output -- Moving Boundary PDE Model Implementation -- Moving Boundary PDE Model Output -- Index
    Abstract: The focus of this book is a detailed discussion of a dual cancer vaccine (CV)-immune checkpoint inhibitor (ICI) mathematical model formulated as a system of partial differential equations (PDEs) defining the spatiotemporal distribution of cells and biochemicals during tumor growth. A computer implementation of the model is discussed in detail for the quantitative evaluation of CV-ICI therapy. The coding (programming) consists of a series of routines in R, a quality, open-source scientific computing system that is readily available from the internet. The routines are based on the method of lines (MOL), a general PDE algorithm that can be executed on modest computers within the basic R system. The reader can download and use the routines to confirm the model solutions reported in the book, then experiment with the model by varying the parameters and modifying/extending the equations, and even studying alternative models with the PDE methodology demonstrated by the CV-ICI model. Spatiotemporal Modeling of Cancer Immunotherapy: Partial Differential Equation Analysis in R facilitates the use of the model, and more generally, computer- based analysis of cancer immunotherapy mathematical models, as a step toward the development and quantitative evaluation of the immunotherapy approach to the treatment of cancer
    Pages: VIII, 112 p. 70 illus. : online resource.
    ISBN: 9783030190804
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  • 51
    Keywords: Oncology ; Medical virology ; Microbiology ; Zoology ; Cancer Research ; Virology ; Microbiology ; Zoology ; Springer eBooks
    Description / Table of Contents: Overview of virus infection and tumorigenesis -- Marine viruses -- Marine invertebrate stress responses to virus infection -- The roles of microRNAs in antiviral immunity of marine invertebrates -- Marine microbe stress responses to bacteriophage infection -- Roles of microbial metabolites in bacteriophage-microbe interactions -- Tumorigenesis and metabolism disorder -- Effects of microRNAs from marine invertebrate stress responses to virus infection on tumorigenesis -- Anti-tumor activities of secondary metabolites from marine microbe stress responses to virus infection
    Abstract: This book reviews the latest research on the molecules and mechanisms of marine host stress responses to viral infections and tumorigenesis. It offers an overview of the state of the art in the field as well as future directions. Metabolism disorder is a characteristic of tumorigenesis. Since viruses complete their life cycle in host cells, such infections cause metabolic disorders in the host. As such, the mechanisms of virus pathogenesis and tumor progression are similar or even identical. In essence, the role of antiviral molecules is to maintain the metabolic homeostasis of infected host cells, and the antiviral molecules induced by virus infection may play an important role in antitumor pathways, resulting in cancer cell death or restoring the disordered metabolism of cancer cells. The molecules generated during host stress responses to viruses can also contribute to the antitumor mechanisms in humans. However, the relationship between host stress responses to virus infection and tumorigenesis has not been extensively explored. In recent years, studies have shown that marine host stress responses to viral invasion can be good models for exploring human antitumor mechanisms. Stimulating further research in the field, this book offers graduate students and researchers with comprehensive insights into host stress responses to viral invasion and tumor progression. It is also a valuable resource for those working in the pharmaceutical industry interested in drug discovery based on molecules derived from host stress responses to viral infection
    Pages: V, 318 p. 8 illus., 7 illus. in color. : online resource.
    ISBN: 9789811361982
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  • 52
    Keywords: Stem Cells ; Oncology ; Cell Cycle ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Cell Cycle Analysis ; Springer eBooks
    Description / Table of Contents: Multilayered heterogeneity of glioblastoma stem cells: biological and clinical significance -- Heterogeneity of head and neck squamous cell carcinoma stem cells -- Heterogeneity of small cell lung cancer stem cells -- Heterogeneity of hepatic cancer stem cells -- Heterogeneity and plasticity of breast cancer stem cells -- Heterogeneity of melanoma with stem cell properties -- Heterogeneity of colon cancer stem cells -- Urothelial cancer stem cell heterogeneity -- Acute myeloid leukemia stem cell heterogeneity and its clinical relevance -- Contribution of chronic myeloid leukaemia (CML) as a disease model to define and study clonal heterogeneity -- The heterogeneity of osteosarcoma: the role played by cancer stem cells -- Ovarian cancer stem cell heterogeneity -- Index
    Abstract: This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue, for several types of cancers, e.g. glioblastoma stem cells. Recent developments have revealed completely different roles of distinct stem cells within the same organ. Thus, Stem Cells Heterogeneity in Cancer provides a timely update us on the current information on stem cells heterogeneity in various tissues. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity – Novel Concepts, and Stem Cells Heterogeneity in Different Organs, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians
    Pages: XII, 228 p. 24 illus., 18 illus. in color. : online resource.
    ISBN: 9783030143664
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  • 53
    Keywords: Medicine ; Monoclonal antibodies ; Molecular Medicine ; Antibodies ; Springer eBooks
    Description / Table of Contents: Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes -- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry -- Trimming of MHC Class I Ligands by ERAP Aminopeptidases -- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases -- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays -- In Vitro Studies of MHC Class I Peptide Loading and Exchange -- Measuring Synthesis and Degradation of MHC Class I Molecules -- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules -- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics -- Purification and Identification of Naturally Presented MHC Class I and II Ligands -- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs -- Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies -- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors -- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway -- Evaluating CD8+ T Cell Responses In Vitro -- Recombinant Poxviruses: Versatile Tools for Immunological Assays -- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy -- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases -- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays -- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays -- In Vitro Digestion with Proteases Producing MHC Class II Ligands -- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors -- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading -- Studying MHC Class II Peptide Loading and Editing In Vitro -- Monitoring Antigen Processing for MHC Presentation via Macroautophagy -- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies -- Production of Antigen Specific Human CD4+ T Cell Lines and Clones -- Monitoring Dendritic Cell Activation and Maturation -- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes -- Analyzing Antigen Recognition by Natural Killer T Cells
    Abstract: The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field
    Pages: XV, 458 p. 79 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493994502
    URL: