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  • Springer eBooks  (3,540)
  • Neurologie
  • Theoretische Physik
  • Messtechnik
  • Strömungsmechanik
  • Springer  (3,574)
  • (via Ovid)  (21)
  • Oxford University Press
  • American Institute of Physics (AIP)
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Keywords
  • 1
    Keywords: Neurology ; Neurobiology ; Neurology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Integrative Biomarkers in Stroke -- Vascular-Related Biomarkers of Ischemic Stroke -- Generation and Applicability of Genetic Risk Scores (GRS) in Stroke -- Neuroplasticity Biomarkers in Experimental Stroke Recovery -- Methods of Mitochondrial and Redox Measurements in Ischemic Stroke -- Blood Biomarkers for Stroke Differentiation -- Laser-Capture Micro-Dissection for Measurement of Angiogenesis after Stroke -- Blood-Borne Biomarkers of Hypertension Predicting Hemorrhagic and Ischemic Stroke -- RNA Gene Expression to Identify the Etiology of Acute Ischemic Stroke: The Biomarkers of Acute Stroke Etiology (BASE) Study -- Neurotoxicity Biomarkers Assays Development -- Glutamate Receptor Peptides as Potential Neurovascular Biomarkers of Acute Stroke -- Antibodies to NMDA Receptors in Cerebral and Spinal Cord Infarctions -- Impaired Retinal Vasoreactivity as an Early Marker of Stroke Risk in Diabetes -- Imaging Biomarkers: Keys to Decision Making in Stroke -- Neuroimaging Methods for Acute Stroke Diagnosis and Treatment -- Ultrasound Assessments of Risk for TIA and Stroke in Vascular Surgery -- Preoperative and Intraoperative Markers of Cerebral Ischemia -- Time is Brain: the Prehospital Phase and the Mobile Stroke Unit -- Acute Clinical Intervention and Chronic Management of Cerebral Vascular Accident -- Trends in Biomarkers Development for Stroke
    Abstract: This volume presents the latest data on recent achievements in new and emerging technologies for stroke biomarkers and innovations in stroke assessment. The topics discussed in this book explore the role of upcoming biomarkers in different types of stroke, and explores techniques that will allow researchers to be more effective when approaching clinical management and patient care. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to ensure successful results in the laboratory. Cutting-edge and comprehensive, Stroke Biomarkers is a valuable resource for both experimental and clinical scientists interested in expanding their knowledge in the field of stroke research
    Pages: XVII, 425 p. 80 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493996827
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  • 2
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
    Abstract: The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
    Pages: XI, 247 p. 30 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998654
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  • 3
    Keywords: Bacteriology ; Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing of Staphylococcus aureus -- Genetic Manipulations of Staphylococcal Chromosomal DNA -- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology -- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus -- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus -- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology -- Metabolomic Profiling of Staphylococcus aureus -- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells -- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging -- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options -- Animal Models for Drug Development for MRSA
    Abstract: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens
    Pages: XI, 268 p. 35 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998494
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  • 4
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook -- Immunophenotyping by Mass Cytometry -- Fluorescent Cell Barcoding for Immunophenotyping -- Immunophenotyping using Dried and Lyophilized Reagents -- Guidelines for Gating Flow Cytometry Data for Immunological Assays -- Strategies and Techniques for NK Cell Phenotyping -- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue -- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells -- Immunophenotyping of Human Regulatory T Cells -- Immunophenotyping of Human Innate Lymphoid Cells -- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis -- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles -- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells -- Quality Control of Immunophenotyping -- Immunophenotyping of Acute Myeloid Leukemia -- Immunophenotyping of Acute Lymphoblastic Leukemia -- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia -- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
    Abstract: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field
    Pages: X, 356 p. 94 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493996506
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  • 5
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures
    Abstract: This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research
    Pages: XV, 363 p. 72 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493976171
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  • 6
    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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  • 7
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Anesthesiology ; Neurosciences ; Anesthesiology ; Springer eBooks
    Description / Table of Contents: The Challenge of Accidental Awareness during General Anesthesia -- Impact of Anesthetics on Brain Electrical Activity and Principles of pEEG-Based Monitoring during General Anethesia -- Perioperative Monitoring of Autonomic Nervous Activity -- Monitoring Cerebral Oximetry by Near Infrared Spectroscopy (NIRS) in Anaesthesia and Critical Care: Progress and Perspectives -- Post-Traumatic Stress Disorder following Intraoperative Awareness -- Mechanisms of Action of Inhaled Volatile General Anesthetics: Unconsciousness at the Molecular Level -- Intravenous Hypnotic Agents: From Binding Sites to Loss of Consciousness -- Pharmacological Considerations for the Use of General Anesthetics in the Elderly -- Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies -- Postoperative Cognitive Function following General Anesthesia in Children -- The Challenge of Opioid-Free Anesthesia -- New Insights into the Pharmacology of Dexmedetomidine and Open Issues for Neurosurgical Procedures -- Transgenic Mouse Models, General Anesthetics and Alzheimer Disease: Findings from Preclinical Studies -- The Biochemical Basis of Delirium -- Postoperative Delirium and Postoperative Cognitive Dysfunction
    Abstract: This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology. Furthermore, chapters in this book cover technical topics such as preoperative monitoring of autonomic nervous activity; EEG based monitoring of general anesthesia; monitoring cerebral oximetry by near infrared spectroscopy; pharmacological issues of anesthesia interest; and optimization of perioperative course through the latest techniques such as the opioid-free anesthesia. Finally, this book offers a comprehensive and up-to-date discussion on the phenomenon of general anesthesia awareness and its potential psychological sequelae. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, General Anesthesia Research is a valuable resource for novice and expert anesthesiologists who want to learn more about the important advances made in this developing field
    Pages: XIV, 256 p. 15 illus., 14 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998913
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  • 8
    Keywords: Mass spectrometry ; Proteomics ; Mass spectrometry ; Proteomics ; Springer eBooks
    Description / Table of Contents: Introduction to Mass Spectrometry-based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Comparing Peptide Spectra Matches Across Search Engines -- Calculation of False Discovery Rate for Peptide and Protein Identification -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Interpretation of Tandem Mass Spectra of Posttranslationally Modified Peptides -- Solution to Dark Matter Identified by Mass-tolerant Database Search -- Phosphoproteomics Profiling to Identify Altered Signaling Pathways and Kinase-targeted Cancer Therapies -- Mass Spectrometry Based Characterization of Ub- and UbL-modified Proteins -- Targeted Proteomics as a Tool for Quantifying Urine-based Biomarkers -- Data Imputation in Merged Isobaric Labelling-based Relative Quantification Datasets -- Clustering Clinical Data in R -- Review of Issues and Solutions to Data Analysis Reproducibility and Data Quality in Clinical Proteomics -- Review of Batch Effects Prevention, Diagnostics, and Correction Approaches -- Using the Object-oriented PowerShell for Simple Proteomics Data Analysis -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data
    Abstract: The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics. In contrast to the previous editions, this third edition aims to provide the most relevant computational methods, focusing on computational concepts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Data Analysis in Proteomics, Third Edition to ensure successful results in the further study of this vital field
    Pages: X, 445 p. 136 illus., 94 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493997442
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  • 9
    Keywords: Neurobiology ; Developmental Biology ; Neurosciences ; Laboratory animals ; Neurobiology ; Developmental Biology ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra -- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis -- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis -- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System -- Immunostaining of the Embryonic and Larval Drosophila Brain -- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila -- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling -- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update -- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain -- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila -- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development -- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum -- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium -- X-Ray Microscopy of the Larval Crustacean Brain -- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain -- Immunostainings in Nervous System Development of the Nematode C. elegans -- Methods in Brain Development of Molluscs -- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization -- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum -- In Situ Hybridization and Immunostaining of Xenopus Brain -- Morpholino Studies in Xenopus Brain Development -- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish -- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish -- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain -- Gene Silencing in Chicken Brain Development -- Transplantation of Neural Tissue: Quail-Chick Chimeras -- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development -- The Cre/Lox System to Assess the Development of the Mouse Brain -- In Utero Electroporation to Study Mouse Brain Development
    Abstract: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain
    Pages: XIV, 527 p. 116 illus., 96 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493997329
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  • 10
    Keywords: Microbial genetics ; Microbial genomics ; Bacteriology ; Microbial Genetics and Genomics ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples -- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health -- Detection, Isolation, and Characterization of Plasmids in the Environment -- Bacteriophage Isolation and Characterization: Phages of Escherichia coli -- Detection and Characterization of Transposons in Bacteria -- Measuring Plasmid Conjugation Using Antibiotic Selection -- Measuring Plasmid Conjugation Using Fluorescent Reporters -- Methods to Quantify DNA Transfer in Enterococcus -- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis -- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases -- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis -- First Biochemical Steps on Bacterial Transposition Pathways -- Natural Transformation in Escherichia coli -- Integron Identification in Bacterial Genomes and Cassette Recombination Assays -- Methods to Identify and Analyze Vesicle-Protected DNA Transfer -- Measuring Plasmid Stability in Gram-Negative Bacteria -- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems -- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements -- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan -- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS) -- MOBscan: Automated Annotation of MOB Relaxases -- Plasmid Typing and Classification -- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets -- Statistical Analysis of Accessory Genome -- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits -- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria -- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria -- Conjugative Assembly Genome Engineering (CAGE)
    Abstract: This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality
    Pages: XV, 413 p. 62 illus., 52 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998777
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  • 11
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example
    Abstract: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field
    Pages: XIV, 405 p. 76 illus., 66 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998180
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  • 12
    Keywords: Antibodies ; Pharmacology ; Antibodies ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Overview of the Current ADC Discovery Landscape -- Pushing the Envelope: Advancement of ADCs Outside of Oncology -- Conjugations to Endogenous Cysteine Residues -- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies -- Transglutaminase-Mediated Conjugations -- Click Chemistry Conjugations -- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates -- Bridged Cysteine Conjugations -- Antibody Conjugations via Glycosyl Remodeling -- ADC Analysis by Hydrophobic Interaction Chromatography -- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs -- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction -- Analysis of ADCs by Native Mass Spectrometry -- High Resolution Characterization of ADCs by Orbitrap LCMS -- Conjugation Site Analysis by MS/MS Protein Sequencing -- Conjugation Site Analysis of Lysine-Conjugated ADCs -- Characterization of ADCs by Capillary Electrophoresis -- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry -- Purification of ADCs by Hydrophobic Interaction Chromatography -- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations -- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions -- Biophysical Methods for Characterization of Antibody Drug Conjugates -- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines -- LC/MS Methods for Studying Lysosomal ADC Catabolism -- Assessing ADC Plasma Stability by LC/MS Methods -- Determination of ADC Concentration by Ligand-Binding Assays
    Abstract: This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the €œáctivation barrieŕ€ when undertaking a new discipline, and provides a ́€œtoolbox́€ for the next generation of ADC scientists
    Pages: XI, 373 p. 138 illus., 109 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999293
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  • 13
    Keywords: Biomedical Engineering ; Cell Biology ; Bioinformatics ; Biology—Technique ; Systems Biology ; Biological systems ; Biomedical Engineering/Biotechnology ; Cell Biology ; Computational Biology/Bioinformatics ; Biological Techniques ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB: Image Analysis & Brownian Motion -- Resolving the process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM)
    Abstract: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images
    Pages: X, 170 p. 69 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030223861
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  • 14
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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  • 15
    Keywords: Infectious Diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Tour de Herpes: Cycling through the Life and Biology of HSV-1 -- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology -- Herpes Simplex Virus Growth, Preparation, and Assay -- Engineering HSV-1 Vectors for Gene Therapy -- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- oHSV Genome Editing by Means of galK Recombineering -- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein -- CRISPR/Cas9-Based Genome Editing of HSV -- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH) -- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing -- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals -- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry -- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells -- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot -- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane -- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination -- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1 -- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
    Abstract: This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV
    Pages: XIV, 457 p. 60 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998142
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  • 16
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels
    Abstract: This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 293 p. 46 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998456
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  • 17
    Keywords: Molecular Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent
    Abstract: This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field
    Pages: X, 300 p. 50 illus., 32 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998821
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  • 18
    Keywords: Human Genetics ; Proteins  ; Human Genetics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies
    Abstract: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field
    Pages: XII, 445 p. 86 illus., 61 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998531
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  • 19
    Keywords: Proteins  ; Biotechnology ; Nanotechnology ; Protein Science ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Introduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins
    Abstract: This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field
    Pages: XIII, 331 p. 84 illus., 64 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998692
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  • 20
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Experimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Abstract: This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study
    Pages: XI, 286 p. 65 illus., 53 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997848
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  • 21
    Keywords: Biochemistry ; RNA-ligand interactions ; Protein Science ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Predicting Protein-Protein Interactions using SPRINT -- Automated Extraction and Visualization of Protein-Protein Interaction Networks and Beyond: A Text Mining Protocol -- Construction of Functional Protein Networks using Domain Profile Associations -- Reconstruction of Protein-Protein Interaction Networks using Homology-Based Search: Application to the Autophagy Pathway of Ageing in Podospora anserina -- Predicting Interacting Protein Pairs by Co-Evolutionary Paralog Matching -- A Web-Based Protocol for Inter-Protein Contact Prediction by Deep Learning -- Visual Analysis of Protein-Protein Interaction Docking Models using COZOID Tool -- Path-LZerD: Predicting Assembly Order of Multimeric Protein Complexes -- Embedding Alternative Conformations of Proteins in Protein-Protein Interaction Networks -- Informed Use of Protein-Protein Interaction Data: A Focus on the Integrated Interactions Database (IID) -- Generation and Interpretation of Context-Specific Human Protein-Protein Interaction Networks with HIPPIE -- Explore Protein-Protein Interactions for Cancer Target Discovery using the OncoPPi Portal -- Perform Pathway Enrichment Analysis using ReactomeFIViz -- De Novo Pathway Enrichment with KeyPathwayMiner -- De Novo Pathway-Based Classification of Breast Cancer Subtypes -- Vienna Graph Clustering -- On TD-WGcluster - Theoretical Foundations and Guidelines for the User -- An Introductory Guide to Aligning Networks using SANA, the Simulated Annealing Network Aligner
    Abstract: This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: XI, 286 p. 97 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998739
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  • 22
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 23
    Keywords: Cell Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Purinergic Signaling -- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling -- Agonists and Antagonists for Purinergic Receptors -- Homology Modelling of P2X Receptors -- Using RNA Interference for Purinoceptor Knock Down in vivo -- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos -- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues -- Flow Cytometry of Membrane Purinoreceptors -- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation -- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking -- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer -- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy -- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor -- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse -- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging -- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels -- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader -- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes -- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells -- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages -- Automated Planar Patch-clamp Recording of P2X Receptors -- Controlling Engineered P2X Receptors with Light -- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment -- Assays to Measure Purinoceptor Pore Dilation -- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization -- Measuring Leukocyte Migration to Nucleotides -- Assessment of Cell Adhesion after Purinoceptor Activation
    Abstract: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines
    Pages: XII, 360 p. 82 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997176
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  • 24
    Keywords: Biochemistry ; Cell Membranes ; Biochemistry, general ; Membrane Biology ; Biological and Medical Physics, Biophysics ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- 1. Understanding Membranes and Vesicles: A Personal Recollection of the Last Two Decades -- 2. Advanced Concepts and Perspectives of Membrane Physics -- Part 2: Membranes by the Numbers -- 3. Membranes by the Numbers -- Part 3: Spatial Heterogeneities in Biomembranes -- 4. LIPID RAFTS – A PERSONAL ACCOUNT -- 5. Theories of Equilibrium In homogeneous Fluids -- 6. Critical lipidomics: The consequences of lipid miscibility in biological membranes -- 7. Lateral diffusion in heterogeneous cell membranes -- 8. Mechanical factors affecting the mobility of membrane proteins -- 9. Membrane domains under cellular recycling -- 10. Protein Pattern Formation -- Part 4: Biomembrane Mechanics and Consequences for their Functions -- 11. Biomembrane mechanical properties direct diverse cell functions -- 12. Spontaneous and intrinsic curvature of lipid membranes: back to the origins -- 13. Membrane-mediated interactions -- 14. Simulating protein-mediated membrane remodeling at multiple scales -- 15. Mechanosensitivity of membrane budding and tracking Lionel Foret -- 16. Common energetic and mechanical features of membrane fusion and fission machineries -- 17. Interaction of particles and pathogens with biological membranes -- 18. Adhesion of biological membranes -- 19. Spatial and mechanical aspects of signal transduction in the cell membrane -- 20. Protein-Induced morphological deformations of biomembranes -- 21. Fluctuations in active membranes
    Abstract: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike
    Pages: X, 623 p. 167 illus., 144 illus. in color. : online resource.
    ISBN: 9783030006303
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  • 25
    Keywords: Agriculture ; Plant breeding ; Plant genetics ; Nutrition ; Agriculture ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Nutrition ; Springer eBooks
    Description / Table of Contents: Preface -- Foreword -- 1. Biofortification of Staple Food crops: An overview -- 2. Marker assisted Breeding for improving the cooking and eating quality of rice -- 3. Improving the nutritional value of potatoes by conventional breeding and genetic modification -- 4. Conventional and molecular breeding approaches for biofortification of pearl millet -- 5. Genetic approaches to improve common bean nutritional quality: current knowledge and future perspectives -- 6. Marker assisted breeding for Beta carotene rich Maize Hybrids -- 7. Recent Advances in Breeding for Modified Fatty Acid Profile in Soybean Oil -- 8. Breeding dry beans with improved cooking and canning quality traits -- 9. Genomic approaches for Biofortification of wheat grain with iron and zinc -- 10. Current Breeding Approaches for Developing Rice with Improved Grain and Nutritional Qualities -- 11. Quality Protein Maize for Nutritional Security -- 12. Genetic improvement for end use quality in wheat -- 13. The use of modern molecular biology and biotechnology tools for improving the Quality value of oilseed Brassicas -- Index
    Abstract: Development of superior crops that have consistent performance in quality and in quantity has not received the same emphasis in the field of genetics and breeding as merited. Specialty trait requires special focus to propagate. Yet basic germplasm and breeding methodologies optimized to improve crops are often applied in the development of improved specialty types. However, because of the standards required for specialty traits, methods of development and improvement are usually more complex than those for common commodity crops. The same standards of performance are desired, but the genetics of the specialty traits often impose breeding criteria distinct from those of non-specialty possessing crops. Specifically, quality improvement programs have unique characteristics that require careful handling and monitoring during their development for specific needs. Adding value either via alternative products from the large volumes of grain produced or development of specialty types is of interest to producers and processors. This work assimilates the most topical results about quality improvement with contemporary plant breeding approaches.The objective of this book is to provide a summary of the germplasm, methods of development, and specific problems involved for quality breeding. In total, fourteen chapters, written by leading scientists involved in crop improvement research, provide comprehensive coverage of the major factors impacting specialty crop improvement
    Pages: XVII, 275 p. 26 illus., 17 illus. in color. : online resource.
    ISBN: 9783030046095
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  • 26
    Keywords: Biological models ; Proteomics ; Gene Expression ; Systems Biology ; Proteomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: 1. The Potential of €˜Ómics to Link Lipid Metabolism and Genetic and Comorbidity Risk Factors of Alzheimeŕ€™s Disease in African Americans -- 2. The Role of Biomarkers in Alzheimeŕ€™s Disease Drug Development -- 3. Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- 4. Lymphocytes, Platelets, Erythrocytes and Exosomes as Possible Biomarkers for Alzheimer's Disease Clinical Diagnosis -- 5. Genetic Risk Factors for Alzheimer Disease: Emerging Roles of Microglia in Disease Pathomechanisms -- 6. Neuroimaging Studies of Cognitive Function in Schizophrenia -- 7. The Role of Biomarkers in Psychiatry -- 8. Interactome Studies of Psychiatric Disorders -- 9. MicroRNAs in Major Depressive Disorder -- 10. Proteomic Markers for Depression -- 11. Advances in Biomarker Studies in Autism Spectrum Disorders -- 12. Proteomic Investigations of Autism Spectrum Disorder: Past Findings, Current Challenges and Future Prospects -- 13. Role of the Gut Microbiome in Autism Spectrum Disorders -- 14. Metabolomic Biomarkers in Mental Disorders: Bipolar Disorder and Schizophrenia -- 15. Early Detection and Treatment of Patients with Alzheimeŕ€™s Disease: Future Perspectives
    Abstract: This book includes a series of reviews on general aspects of biomarker use in the study of psychiatric and neurodegenerative diseases and the development of medications involved in their treatment. It describes the pros and cons of the various approaches and covers the successes and failures in this research field. It is only by a thorough understanding of the shortcomings that progress can be made. The overall goal is to facilitate the understanding and treatment of these disorders, by providing a viable mechanism of catching up with other areas of modern medicine, such as diabetes and heart disease. Finally, it is anticipated that the development and application of valid biomarker tests and the leveraging of novel drug targets will help the fields of psychiatry on neurodegenerative disorders move into the area of personalized medicine where the right patients can receive the right medication at the right time for the best possible outcome
    Pages: XIII, 328 p. 28 illus., 26 illus. in color. : online resource.
    ISBN: 9783030055424
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  • 27
    Keywords: Morphology (Animals) ; Evolution (Biology) ; Paleontology  ; Animal Anatomy / Morphology / Histology ; Evolutionary Biology ; Paleontology ; Springer eBooks
    Description / Table of Contents: Evolution of Chordate Cardiopharyngeal Muscles and the Origin of Vertebrate Head Muscles -- Early Vertebrates and the Emergence of Jaws -- Cranium, Cephalic Muscles, and Homologies in Cyclostomes -- Heads, Jaws and Muscles in Chondrichthyans Actinopterygians: Head, Jaws and Muscles -- Sarcopterygian fishes, the “lobe-fins” -- Diversity of Heads, Jaws, and Cephalic Muscles in Amphibians -- Evolution, Diversity, and Development of the Craniocervical System in Turtles with Special Reference to Jaw Musculature -- An Integrative View of Lepidosaur Cranial Anatomy, Development and Diversification -- The Skull and Head Muscles of Archosauria -- The Origin and Evolution of Mammalian Head Muscles, with Special Emphasis on the Facial Myology of Primates and Modern Humans
    Abstract: The vertebrate head is the most complex part of the animal body and its diversity in nature reflects a variety of life styles, feeding modes, and ecological adaptations. This book will take you on a journey to discover the origin and diversification of the head, which evolved from a seemingly headless chordate ancestor. Despite their structural diversity, heads develop in a highly conserved fashion in embryos. Major sensory organs like the eyes, ears, nose, and brain develop in close association with surrounding tissues such as bones, cartilages, muscles, nerves, and blood vessels. Ultimately, this integrated unit of tissues gives rise to the complex functionality of the musculoskeletal system as a result of sensory and neural feedback, most notably in the use of the vertebrate jaws, a major vertebrate innovation only lacking in hagfishes and lampreys. The cranium subsequently further diversified during the major transition from fishes living in an aquatic environment to tetrapods living mostly on land. In this book, experts will join forces to integrate, for the first time, state-of-the-art knowledge on the anatomy, development, function, diversity, and evolution of the head and jaws and their muscles within all major groups of extant vertebrates. Considerations about and comparisons with fossil taxa, including emblematic groups such as the dinosaurs, are also provided in this landmark book, which will be a leading reference for many years to come
    Pages: XIV, 303 p. 95 illus., 59 illus. in color. : online resource.
    ISBN: 9783319935607
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  • 28
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Normal Growth in PKU Patients Under Low-Protein Diet in a Single-Center Cross-Sectional Study -- Serial Magnetic Resonance Imaging and 1H-Magnetic Resonance Spectroscopy in GABA Transaminase Deficiency: A Case Report.-Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment -- Cognitive and Behavioural Outcomes of Paediatric Liver Transplantation for Ornithine Transcarbamylase Deficiency -- Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation -- Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice -- Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period -- Severe Leukoencephalopathy with Clinical Recovery Caused by Recessive BOLA3 Mutations -- Neonatal Onset Interstitial Lung Disease as a Primary Presenting Manifestation of Mucopolysaccharidosis Type I -- A Middle Eastern Founder Mutation Expands the Genotypic and Phenotypic Spectrum of Mitochondrial MICU1 Deficiency: A Report of 13 Patients -- Disruption of the Responsible Gene in a Phosphoglucomutase 1 Deficiency Patient by Homozygous Chromosomal Inversion -- Evaluation of Disease Lesions in the Developing Canine MPS IIIA Brain -- Extrapolation of Variant Phase in Mitochondrial Short-Chain Enoyl-CoA Hydratase (ECHS1) Deficiency -- RFT1-CDG: Absence of Epilepsy and Deafness in Two Patients with Novel Pathogenic Variants -- Short-Term Administration of Mycophenolate Is Well-Tolerated in CLN3 Disease (Juvenile Neuronal Ceroid Lipofuscinosis)
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 35 illus., 20 illus. in color. : online resource.
    ISBN: 9783662586143
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  • 29
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 30
    Keywords: Medicine ; Oncology ; Molecular Medicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Amplicon-based Targeted Next Generation Sequencing of Formalin Fixed Paraffin Embedded Tissue -- Library preparation using FFPE-derived Tumor DNA for High Throughput Hybridization-based Targeted or Exome Sequencing -- Bioinformatics Basics for High Throughput Hybridization-based Targeted DNA Sequencing From FFPE-Derived Tumor Specimens: From Reads to Variants -- Annotation of Variant Data from High Throughput DNA Sequencing from Tumor Specimens: Filtering Strategies to Identify Driver Mutations -- Biological Interpretation of Complex Genomic Data -- Clinical Validation of Targeted Solid Tumor Profiling -- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors -- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation -- Overview of Fusion Detection Strategies Using Next Generation Sequencing -- Clinical Application of Fusion Gene Detection Using Next-generation Sequencing and the NanoString Technology -- Pipeline for Integrated Microarray Expression Normalization Toolkit (PIMENTo) for Tumor Microarray Profiling Experiment -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: Overview of Library Preparation Methods -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: From Raw Data to Systems Level Analyses -- Methylation Analysis using Microarrays: Analysis and Interpretation -- High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) -- A Pipeline for ctDNA Detection Following Primary Tumor Profiling Using A Cancer-related Gene Sequencing Panel -- Single-cell Analysis of Circulating Tumor Cells
    Abstract: This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field
    Pages: XI, 268 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493990047
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  • 31
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 32
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction -- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction -- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells -- Isolation and Characterization of Cutaneous Epithelial Stem Cells -- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin -- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells -- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis -- Isolation and Cultivation of Epidermal (Stem) Cells -- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen -- Isolation and Cultivation of Skin-Derived Precursors -- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells -- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor -- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair -- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet -- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering -- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration -- Skin Wound Healing: Refractory Wounds and Novel Solutions -- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice -- Melanoblasts as Multipotent Cells in Murine Skin -- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro -- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells -- Isolating Immune Cells from Mouse Embryonic Skin -- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells -- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy -- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications -- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis -- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells -- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research -- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma -- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells -- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
    Abstract: This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells
    Pages: XV, 463 p. 105 illus., 76 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988709
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  • 33
    Keywords: Oncology ; Immunology ; Cancer Research ; Immunology ; Springer eBooks
    Description / Table of Contents: Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry
    Abstract: This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies
    Pages: XI, 225 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493989799
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  • 34
    Keywords: Microbiology ; Microbial genetics ; Microbial genomics ; Biomedical Engineering ; Eukaryotic Microbiology ; Microbial Genetics and Genomics ; Applied Microbiology ; Medical Microbiology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Industrial Applications and Model Systems -- Physiological genomics of multistress resistance in the yeast cell model and factory: focus on MDR/MXR transporters -- Mechanisms of yeast adaptation to wine fermentations -- Development of robust yeast strains for lignocellulosic bio-refineries based on genome-wide studies -- Physiological genomics of the highly weak-acid-tolerant food spoilage yeasts of Zygosaccharomyces bailii sensu lato -- Yeast genome-scale metabolic models for simulating genotype-phenotype relations -- Human Health and Disease -- Emerging mechanisms of drug resistance in Candida albicans -- Genome-wide response to drugs and stress in the pathogenic yeast Candida glabrata -- Lipidomics approaches: applied to the study of pathogenesis in Candida species -- Yeast at the forefront of research on ageing and age-related diseases
    Abstract: This book discusses genome-based strategies to provide a holistic understanding of yeasts in Human Health and as model organisms in basic research or industrial production. Using numerous Saccharomyces cerevisiae strains and various non-conventional yeast species isolated from diverse origins, it describes essential biological processes, the biotechnological exploitation of yeast and pathogenesis control. It also demonstrates how functional and comparative genomics and the development of genome engineering tools are used in modern yeast research. The use of yeasts as experimental eukaryotic models increasingly gained prominence when several Nobel Prizes in Physiology/Medicine and Chemistry were awarded for innovative research, using yeast strains to elucidate molecular mechanisms in a wide range of human physiological processes and diseases, such as autophagy, cell cycle regulation and telomerase activity. This book offers useful insights for scientists in yeast research, clinical scientists working with yeast infectious models and for industrial researchers using applied microbiology
    Pages: V, 242 p. 37 illus., 29 illus. in color. : online resource.
    ISBN: 9783030130350
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  • 35
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Left Ventricular Strain and Relaxation are Independently Associated with Renal Cortical Perfusion in Hypertensive Patients -- Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: a Bispectral Analysis -- Influence of Heart Rate, Age, and Gender on Heart Rate Variability in Adolescents and Young Adults -- Hand-Foot Syndrome and Progression-Free Survival in Patients Treated with Sunitinib for Metastatic Clear Cell Renal Cell Carcinoma -- Elastography in the Diagnosis of Pancreatic Malignancies -- Factors Affecting Health-Related Quality of Life in Liver Transplant Patients -- Phagocytosis and Autophagy in THP-1 cells Exposed to Urban Dust: Possible Role of LC3€“Ássociated Phagocytosis and Canonical Autophagy -- Gendeŕ€“Dependent Responses of Growth and Insuliń€“like Growth Factoŕ€“1 to Growth Hormone Therapy in Prepubertal Growth Hormone Deficient Children -- Vitamin D and Calcium Homeostasis in Infants with Urolithiasis -- Morphometric Analysis of the Lumbar Vertebrae Concerning the Optimal Screw Selection for Transpedicular Stabilization -- Stress and Dehumanizing Behaviors of Medical Staff toward Patients -- Biological and Social Determinants of Maximum Oxygen Uptake in Adult Men
    Abstract: This book focuses on issues in clinical practice and research that are of general interest. The articles primarily focus on understanding the pathogenic mechanisms of diseases, their prevention, and therapy. The topics addressed include cardiovascular regulation with regard to blood pressure and heart rate variability, and to coupling blood pressure changes with subarachnoid fluid oscillations. In addition, the book discusses recent advances in the diagnostics of and targeted molecular therapy for renal and pancreatic malignancies, growth disorders, vitamin D and calcium homeostasis in children in the context of neonatal urolithiasis, and neurosurgical interventions in multifarious age-related diseases of the vertebrae. Neuropsychological aspects of patients’ quality of life and of shaping medical staff’s attitude toward patients are also addressed. The respective articles are intended to build a bridge between basic and clinical research. Further, the book enhances the current body of knowledge on diagnostics and patient treatment and offers valuable new perspectives on practical clinical issues. As such, it offers a unique resource for clinicians, family physicians, medical scholars, and professionals engaged in patient management
    Pages: VI, 114 p. 20 illus. in color. : online resource.
    ISBN: 9783030129231
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  • 36
    Keywords: Otorhinolaryngology ; Neurosciences ; Otorhinolaryngology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Visual Influence on Auditory Perception -- Cue Combination Within a Bayesian Framework -- Toward a Model of Auditory-Visual Speech Intelligibility -- An Object-Based Interpretation of Audiovisual Processing -- Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway -- Multisensory Processing in the Auditory Cortex -- Audiovisual Integration in the Primate Prefrontal Cortex -- Using Multisensory Integration to Understand the Human Auditory Cortex -- Combining Voice and Face Content in the Primate Temporal Lobe -- Neural Network Dynamics and Audiovisual Integration -- Cross-Modal Learning in the Auditory System -- Multisensory Processing Differences in Individuals with Autism Spectrum Disorder
    Abstract: Auditory behavior, perception, and cognition are all shaped by information from other sensory systems. This volume examines this multi-sensory view of auditory function at levels of analysis ranging from the single neuron to neuroimaging in human clinical populations. Visual Influence on Auditory Perception Adrian K.C. Lee and Mark T. Wallace Cue Combination within a Bayesian Framework David Alais and David Burr Toward a Model of Auditory-Visual Speech Intelligibility Ken W. Grant and Joshua G. W. Bernstein An Object-based Interpretation of Audiovisual Processing Adrian K.C. Lee, Ross K. Maddox, and Jennifer K. Bizley Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway Shawn M. Willett, Jennifer M. Groh, Ross K. Maddox Multisensory Processing in the Auditory Cortex Andrew J. King, Amy Hammond-Kenny, Fernando R. Nodal Audiovisual Integration in the Primate Prefrontal Cortex Bethany Plakke and Lizabeth M. Romanski Using Multisensory Integration to Understand Human Auditory Cortex Michael S. Beauchamp Combining Voice and Face Content in the Primate Temporal Lobe Catherine Perrodin and Christopher I. Petkov Neural Network Dynamics and Audiovisual Integration Julian Keil and Daniel Senkowski Cross-Modal Learning in the Auditory System Patrick Bruns and Brigitte Röder Multisensory Processing Differences in Individuals with Autism Spectrum Disorder Sarah H. Baum Miller, Mark T. Wallace Adrian K.C. Lee is Associate Professor in the Department of Speech & Hearing Sciences and the Institute for Learning and Brain Sciences at the University of Washington, Seattle Mark T. Wallace is the Louise B McGavock Endowed Chair and Professor in the Departments of Hearing and Speech Sciences, Psychiatry, Psychology and Director of the Vanderbilt Brain Institute at Vanderbilt University, Nashville Allison B. Coffin is Associate Professor in the Department of Integrative Physiology and Neuroscience at Washington State University, Vancouver, WA Arthur N. Popper is Professor Emeritus and research professor in the Department of Biology at the University of Maryland, College Park Richard R. Fay is Distinguished Research Professor of Psychology at Loyola University, Chicago
    Pages: XVI, 272 p. 70 illus., 49 illus. in color. : online resource.
    ISBN: 9783030104610
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  • 37
    Keywords: Medicine ; Mathematical Statistics ; Biomedicine general ; Statistics and Computing/Statistics Programs ; Springer eBooks
    Description / Table of Contents: Preface -- General Introduction -- Significant and Insignificant Adverse Effect -- Incidence Ratios and Reporting Ratios of Adverse Effects -- Safety Analysis and the Alternative Hypothesis -- Forest Plots of Adverse Effects -- Graphics of Adverse Effects -- Repeated Measures Methods for Testing Adverse Effects -- Benefit Risk Ratios -- Equivalence, Non-inferiority and Superiority Testing of Adverse Effects -- Part II The Analysis of Dependent Adverse Effects -- Independent and Dependent Adverse Effects. Categorical Predictors Assessed as Dependent Adverse Effects. Adverse Effect of the Dependent Type in Crossover Trial -- Confoundings and Interactions Assessed as Dependent Adverse Effects -- Subgroup Characteristics Assessed as Dependent Adverse Effects -- Random Effects Assessed as Dependent Adverse Effects -- Outliers Assessed as Dependent Adverse Effects -- Index
    Abstract: The authors, as professors in statistics at various universities in Europe, are worrried about the poor quality of safety data analysis of clinical trials, despite its importance in drug development and pharmacovigilance. Clinical trials, not adequately addressing safety, are unethical. An effective approach for the purpose is to present summaries of prevalences. In order to estimate the probability, that the differences between treatment and control group did not occur merely by chance, a statistical test can be performed. This pretty crude method has recently be supplemented with better sensitive methodologies, based on machine learning clusters and networks, and multivariate analyses. Another important novelty with safety data analysis is the new insights into hypothesis testing, favoring the alternative hypotheses instead of the null hypotheses. Finally the issue of dependency is addressed. Adverse effects may be either dependent or independent of the main outcome. Dependent adverse effect are dependent not only on the treatment modalities, but also on the outcome of the trials. Random heterogeneities, outliers, confounders, interaction factors are common in clinical trials, and all of them can be considered kinds of adverse effects of the dependent type. Random regressions and analyses of variance, high dimensional clusterings, partial correlations, structural equations models, and other Bayesian methods are helpful for their analysis. The current edition was particularly written for medical and health professionals and students. It provides examples of modern analytic methods so far largely unused. All of the 16 chapters have two core characteristics, first they are for current usage, second they try and tell what readers need to know in order to understand the methods. Step by step analyses are given and self-assessment examples are supplied. Each chapter can be studied as a stand-alone
    Pages: XI, 217 p. 191 illus., 28 illus. in color. : online resource.
    ISBN: 9783030058043
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  • 38
    Keywords: Human Genetics ; Medical virology ; Human Genetics ; Virology ; Springer eBooks
    Description / Table of Contents: 1. Design of AAV vectors for Delivery of RNAi -- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes -- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer -- 4. Quantitative and Digital Droplet-based AAV Genome Titration -- 5. Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual -- 6. In Situ Hybridization for Detection of AAV-Mediated Gene Expression -- 7. Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina -- 8. Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System -- 9. Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-Transgenic Species -- 10. MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain -- 11. AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection -- 12. Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals -- 13. Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia -- 14. SubILM Injection of AAV for Gene Delivery to the Retina -- 15. Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins -- 16. AAV-Mediated Gene Delivery to the Inner Ear -- 17. Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System -- 18. AAV-Mediated Gene Delivery to Taste Cells of the Tongue -- 19. AAV Vectors for Efficient Gene Delivery to Rodent Hearts -- 20. AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods -- 21 -- AAV-Mediated Gene Delivery to the Lung -- 22. Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration -- 23. rAAV-Mediated Gene Delivery to Adipose Tissue -- 24. AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection
    Abstract: This volume provides a complete and timely guide to the use of adeno-associated virus (AAV) vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease
    Pages: XII, 426 p. 62 illus., 50 illus. in color. : online resource.
    ISBN: 9781493991396
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  • 39
    Keywords: Cell Culture ; Laboratory animals ; Cell Culture ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Applicability of Mouse Models to the Study of Human Disease -- Optimizing the Cell Culture Microenvironment -- Propagation and Maintenance of Mouse Embryonic Stem Cells -- Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells -- Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction -- Gene Editing of Mouse Embryonic and Epiblast Stem Cells -- Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood -- Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture -- Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation -- Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo -- Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells -- Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells -- Organotypic Culture of Adult Mouse Retina -- Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes -- Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes -- Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells -- An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells -- Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans -- Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers -- The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential -- Mouse Lung Tissue Slice Culture
    Abstract: This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets
    Pages: XII, 315 p. 64 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990863
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  • 40
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism
    Abstract: This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories
    Pages: XI, 495 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990276
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  • 41
    Keywords: Genetics ; Evolution (Biology) ; Genetics and Genomics ; Evolutionary Biology ; Springer eBooks
    Description / Table of Contents: Setting Up an Ancient DNA Laboratory -- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate -- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step -- Extraction of Highly Degraded DNA from Ancient Bones and Teeth -- Sampling and Extraction of Ancient DNA from Sediments -- Extraction of Ancient DNA from Plant Remains -- DNA Extraction from Keratin and Chitin -- Double-Stranded Library Preparation for Ancient and Other Degraded Samples -- A Method for Single-Stranded Ancient DNA Library Preparation -- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms -- Whole-Genome Capture of Ancient DNA Using Homemade Baits -- Generating RNA Baits for Capture-Based Enrichment -- Hybridization Capture of Ancient DNA Using RNA Baits -- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA -- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis -- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA -- Authentication and Assessment of Contamination in Ancient DNA -- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence
    Abstract: This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab
    Pages: X, 216 p. 15 illus., 5 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991761
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  • 42
    Keywords: Bacteriology ; Enzymes ; Immunology ; Bacteriology ; Enzymology ; Immunology ; Springer eBooks
    Description / Table of Contents: 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus
    Abstract: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets
    Pages: XIII, 312 p. 70 illus., 41 illus. in color. : online resource.
    ISBN: 9781493991549
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  • 43
    Keywords: Cell receptors ; Lipids ; Receptors ; Lipidology ; Springer eBooks
    Description / Table of Contents: Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry
    Abstract: This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond
    Pages: XI, 220 p. 35 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991303
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  • 44
    Keywords: Human Genetics ; Nanotechnology ; Biotechnology ; Toxicology ; Human Genetics ; Nanotechnology ; Biotechnology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection -- Synthesis of Bioreducible Polycations with Controlled Topologies -- Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery -- Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates -- Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems -- Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids -- Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery -- Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation -- Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol -- Layer-By-Layer Assembled Nanoparticles for siRNA Delivery -- Layer-By-Layer Film Engineering for Sequential Gene Delivery -- Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles -- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films -- Lyophilization of Synthetic Gene Carriers -- Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery -- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles -- Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery -- Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity -- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity -- Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS) -- Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining -- Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery -- Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo -- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery -- Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation -- Lipopeptide Delivery of siRNA to the Central Nervous System
    Abstract: This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself
    Pages: XV, 406 p. 107 illus., 71 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990924
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  • 45
    Keywords: Cytology ; Neurosciences ; Cell Biology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System -- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing -- Myelin: Methods for Purification and Proteome Analysis -- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells -- Isolation and Culture of Oligodendrocytes -- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation -- Oligodendrocyte-Neuron Myelinating Co-Culture -- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier -- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices -- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord -- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination -- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination -- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation -- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis -- Conditional Mutagenesis in Oligodendrocyte Lineage Cells -- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage -- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination -- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease -- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes -- Transmission Electron Microscopy of Oligodendrocytes and Myelin -- Toxin-Based Models to Investigate Demyelination and Remyelination -- Magnetic Resonance techniques for Imaging White Matter
    Abstract: This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function
    Pages: XII, 411 p. 65 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990726
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  • 46
    Keywords: Genetics ; Human Genetics ; Medical virology ; Medicine ; Genetics and Genomics ; Human Genetics ; Virology ; Learning & Instruction ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Monogenic Disorders -- Cytogenetics -- Medical Genetics -- Viral Gene Therapy
    Abstract: This textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The “Labster Virtual Lab Experiments” series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn’t have access to. In this book, you’ll learn the fundamental concepts of the genetics of human diseases focusing on: Monogenic Disorders - Cytogenetics - Medical Genetics - Viral Gene Therapy In each chapter, you’ll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you’ll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access to five simulations for the duration of six months. If you’re using the e-book version, you can sign up and buy access to the simulations at www.labster.com/springer. If you like this book, try out other topics in this series, including “Basic Biology”, “Basic Genetics”, and “Basic Biochemistry”. About Labster Labster is a company dedicated to developing virtual lab simulations that are designed to stimulate students’ natural curiosity and highlight the connection between science and the real world. These simulations have been shown to improve the achievement of learning outcomes among students, by making the learning experience more immersive and engaging. The content of this book was created by the Labster team members Dr. Aaron Gardner, Dr. Sarah Stauffer, Dr. Lindsay Petley-Ragan, Philip Wismer, Dewi Ayu Kencana Ungu and Silvia Tjong. You can learn more about Labster and explore the simulations at www.labster.com
    Pages: IX, 76 p. 43 illus. in color. : online resource.
    ISBN: 9783662587447
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  • 47
    Keywords: Medicine ; Toxicology ; Cell Physiology ; Endocrinology ; Metabolic Diseases ; Molecular Medicine ; Pharmacology/Toxicology ; Cell Physiology ; Endocrinology ; Metabolic Diseases ; Springer eBooks
    Description / Table of Contents: Part 1. Origin of Brown and Beige Adipocytes -- 1. Brown Adipose Tissue Development and Metabolism -- 2. Lessons from Cre-Mice and Indicator Mice -- 3. Aging of Brown and Beige/Brite Adipose Tissue -- 4. Adipogenesis in Primary Cell Culture -- 5. In Vitro Models for Study of Brown Adipocyte Biology -- 6. Brown-Like Adipocyte Progenitors Derived from Human iPS Cells: A New Tool for Anti-obesity Drug Discovery and Cell-Based Therapy? -- 7. Brown Adipose Tissue in Human Infants -- Part 2. Molecular Mechanisms of BAT Function and Signaling -- 8. Evolution of UCP1 -- 9. The Mechanism FA-Dependent H+ Transport by UCP1 -- 10. Role of cAMP and cGMP Signaling in Brown Fat -- 11. Fatty Acid Metabolites as Novel Regulators of Non-shivering Thermogenesis -- 12. Regulatory Small and Long Noncoding RNAs in Brite/Brown Adipose Tissue -- 13. Brown Adipokines -- Part 3 Detection of BAT in vivo -- 14. Infrared Thermography -- 15. In Vivo Detection of Human Brown Adipose Tissue During Cold and Exercise by PET/CT -- 16. Techniques and Applications of Magnetic Resonance Imaging for Studying Brown Adipose Tissue Morphometry and Function -- 17. Multispectral Optoacoustic Tomography of Brown Adipose Tissue -- 18. BAT Exosomes: Metabolic Crosstalk with Other Organs and Biomarkers for BAT Activity -- Part 4. Recruitment and Activation of human BAT -- 19. Activation of Human Brown Adipose Tissue (BAT): Focus on Nutrition and Eating -- 20. Translational Aspects of Brown Fat Activation by Food-Derived Stimulants -- 21. Translational Pharmacology and Physiology of Brown Adipose Tissue in Human Disease and Treatment
    Abstract: The main focus of this book is on brown adipose tissue and its metabolic function. The book provides a timely update on the latest research and shows where the field is heading. Brown adipose tissue (BAT) dissipates energy and has received considerable attention in the last few years, having been re-discovered in adult humans in 2007/9. Moreover, BAT might offer a target for novel therapies to address obesity, a health condition that has reached pandemic dimensions
    Pages: VIII, 424 p. 52 illus., 49 illus. in color. : online resource.
    ISBN: 9783030105136
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  • 48
    Keywords: Biomedical Engineering ; Nanotechnology ; Cytology ; Bioethics ; Biomedical Engineering/Biotechnology ; Nanotechnology ; Oxidative Stress ; Bioethics ; Springer eBooks
    Description / Table of Contents: Novel therapeutics and diagnostics strategies based on engineered nanobiomaterials -- Gold Nanostructures for Photothermal Therapy -- Nanomaterials-based siRNA Delivery: Routes of administration, hurdles and role of nanocarriers -- Silver-based Polymeric Nanocomposites as Antimicrobial Coatings for Biomedical Applications -- Recent trends of nano-materials antimicrobial agents -- Therapeutic Leishmaniasis: Recent Advancement and Developments -- Nanomanipulation of consumer goods: Effects on human health and environment
    Abstract: The book introduces the basic concepts of nanotechnology and the various technologies to characterize nanomaterials. It also covers the nanostructural features of mammalian cells/tissues and related nanomechanical properties. In addition, the book comprehensively describes the current state-of-the-art and future perspectives of nanotechnology in biosensors. It also discusses the potential of nanotechnology for delivering the diverse cancer therapeutics and illustrates its limitation due to the potential toxicity associated with oxidative stress. It also highlights the ethical issues and translational aspects related to nanotechnology. Finally, it summarizes the applications of nanotechnology in animal biotechnology, the recent perspectives and future challenges of nanomedicines. The content of the book are beneficial for the undergraduate, postgraduate and doctoral students as well the professionals working in the area of nanotechnology and nanomedicines
    Pages: XII, 254 p. 40 illus., 30 illus. in color. : online resource.
    ISBN: 9789811360046
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  • 49
    Keywords: Biomedical Engineering ; Cytology / Research ; Microbial ecology ; Microbiology ; Plant Diseases ; Biomedical Engineering/Biotechnology ; Biological Techniques ; Microbial ecology ; Microbiology ; Plant Pathology ; Springer eBooks
    Description / Table of Contents: Part I: Root symbioses -- Chapter 1. Synthetic plasmids to challenge symbiotic nitrogen fixation between rhizobia and legumes -- Chapter 2. In vivo analysis of rhizosphere enzyme activities by the use of plastic syringes -- Chapter 3. Characterization of arbuscular mycorrhizal communities in roots of vineyard plants -- Chapter 4. Molecular methods for research on actinorhiza -- Chapter 5. Molecular and functional characterization of beneficial bacteria associated with AMF spores -- Part II: Plant pathogens and microbial plant protection -- Chapter 6. Oomycete-root interactions -- Chapter 7. Exploitation of rhizosphere microbiome services -- Chapter 8. Methods for detecting biocontrol and plant growth-promoting traits in rhizobacteria -- Chapter 9. A split-root method to study systemic and heritable traits induced by Trichoderma in tomato plants -- Part III: Experimental approaches and analytical techniques in rhizosphere biology -- Chapter 10. Using a tri-isotope (13C, 15N, 33P) labelling method to quantify rhizodeposition -- Chapter 11. Microscopic techniques coupled to molecular and genetic approaches to highlight cell-type specific differences in mycorrhizal symbiosis -- Chapter 12. From imaging to functional traits in interactions between roots and microbes -- Chapter 13. Live imaging of arbuscular mycorrhizal symbiosis -- Chapter 14. Microcosm approaches to investigate multitrophic interactions in microbial communities -- Chapter 15. Analysis of common mycorrhizal networks in microcosms
    Abstract: This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation
    Pages: IX, 279 p. 37 illus., 34 illus. in color. : online resource.
    ISBN: 9789811357671
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    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media -- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell -- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell -- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome -- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host -- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome -- Gene Expression Analysis in the Pneumococcus -- Transcriptional Knockdown in Pneumococci using CRISPR Interference -- Protein Expression Analysis by Western Blot and Protein-Protein Interactions -- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony -- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria -- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells -- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae -- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease -- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection -- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo -- The Pneumococcus and Its Critical Role in Public Health -- The Epidemiology and Biostatistics of Pneumococcus
    Abstract: This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field