Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • DKFZ Publication Database  (3)
  • CELL  (3)
  • PROTEIN  (3)
  • PROTEINS  (3)
  • human
  • GENES
  • ELSEVIER ACADEMIC PRESS INC  (2)
  • BLACKWELL PUBLISHING  (1)
Collection
  • DKFZ Publication Database  (3)
Source
Keywords
  • 1
    facet.materialart.
    Unknown
    ELSEVIER ACADEMIC PRESS INC
    Keywords: BIOLOGY ; BINDING ; MOBILITY ; SPECTROSCOPY ; DOMAINS ; sensitivity ; MOTION ; LIVING CELLS ; FLUORESCENCE ; PROTEIN-PROTEIN INTERACTIONS ; Jun ; DNA ; PROTEINS ; PROTEIN ; PATHWAY ; CELLS ; CELL ; Germany ; interactions ; CELL BIOLOGY ; FCS ; interaction ; CROSS-CORRELATION SPECTROSCOPY ; CORRELATION SPECTROSCOPY ; fluorescence correlation spectroscopy ; DIFFUSION ; molecular ; review ; RE ; correlation ; USA ; technique ; FOS ; methods
    Abstract: Fluorescence correlation spectroscopy (FCS) is an emerging technique where the interaction between biomolecules is detected through their correlated motion. It offers the advantage of high (single-molecule) sensitivity; independence of molecular orientation or distance; and simultaneous measurement of molecular interactions, concentrations, and mobilities. Here we introduce the principle of the technique and review some recent examples from the literature where FCS has been used with autofluorescent proteins for measuring protein-protein interactions and mobilities in living cells
    Type of Publication: Book chapter
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: Rb ; PKB/AKT ; lipid ; mechanism of action ; erufosine ; synergistic combinations ; pAkt ; BCR-ABL ; CML ; caspases ; KINASE INHIBITOR ; LEVEL ; INCREASE ; SUBSTRATE ; AGENT ; signaling ; CASPASE ; INHIBITORS ; protein expression ; MAMMALIAN-CELLS ; PROTEIN-KINASE-C ; leukemia ; FUSION ; LINE ; MEMBRANE ; SIGNAL-TRANSDUCTION ; COMPONENT ; ALKYLPHOSPHOCHOLINES ; ANTILEUKEMIC EFFICACY ; CELL-LINE ; chemotherapy ; cell lines ; p27 ; ENTRY ; HEALTH ; ASSAY ; PROGRESSION ; SIGNAL ; treatment ; TYROSINE KINASE INHIBITOR ; CYCLE ; CELL-LINES ; CELL-CYCLE ; cell cycle ; PROTEIN-KINASE ; signal transduction ; INDUCTION ; REDUCTION ; TYROSINE KINASE ; COMBINATION ; KINASE ; PATHWAY ; THERAPY ; PATHWAYS ; CELL ; INHIBITOR ; APOPTOSIS ; EXPRESSION ; CELLS ; DISEASE ; NEW-YORK ; LINES ; cell line ; DRUG ; PROTEINS ; PROTEIN ; TRANSDUCTION ; cell signaling ; MECHANISM ; PATIENT
    Abstract: The ether lipid analog erufosine (erucylphospho-N,N,N,- trimethylpropylammonium, ErPC3) has high activity against leukemic cells without affecting the normal hematopoiesis. It belongs to the group of alkylphosphocholines (APC) that are inhibitors of protein kinase C and phospholipase C. However, the mechanism of action of erufosine remains rather unclear. We focused on combination effects with the tyrosine kinase inhibitor imatinib mesylate (gleevec, former STI-571 or CGP-57148) against two chronic myeloid leukemia (CML)-derived cell lines (K-562 and BV-173). The influence of erufosine on proteins involved in the phosphatidylinositol-3-phosphate pathway and on expression of the retinoblastoma protein Rb was studied, the latter being a key component for cell cycle entry and progression in mammalian cells. The consecutive treatment of K-562 and BV-173 cells with erufosine (2.5, 5, 15, 30 mu M) and imatinib mesylate (0.05, 0.1 mu M) led to synergism as measured by the MTT-dye reduction assay and this is reason to hypothesize that such combinations could be beneficial for relapsed patients with drug-resistant disease. Whole cell lysates from K-562 and BV-173 were investigated for the expression of Rb, PKB/Akt, pAkt, and p27 by Western blot. Erufosine caused decreases of pAkt and CML fusion protein p210 (BCR-ABL) protein expression, but induced the Rb protein expression in K-562 cells. A parallel increase in p27 level was observed after 24 and 48 h treatment. These alterations in signal transduction could be an explanation for the drug interaction found. Furthermore, Rb is a substrate of caspases and is cleaved during apoptosis as already evidenced for BV-173 cells. Our experimental findings suggest that erufosine acts through induction of changes in protein signaling and especially through Rb induction. This unique mode of action makes it an attractive partner for combination therapies, for example, in combination with imatinib mesylate for treatment of CML
    Type of Publication: Book chapter
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: BEHAVIOR ; PHENOTYPE ; BIOLOGY ; IDENTIFICATION ; NUCLEI ; RECOGNITION ; culture ; PHENOTYPES ; PATTERNS ; IMAGES ; CLASSIFICATION ; CELLS ; CELL ; Germany ; evaluation ; human ; ACCURACY ; PROTEIN ; PROTEINS ; GENE ; TOOL ; GENOME-WIDE ; MICROSCOPE IMAGES ; SUBCELLULAR STRUCTURES ; TEXTURE ; Cell nuclei ; CELL BIOLOGY ; RE ; review ; HIGH-THROUGHPUT ; SCREEN ; analysis ; methods ; CELL-NUCLEI ; USA ; HUMAN-CELLS ; TOOLS
    Abstract: High-throughput screens of the gene function provide rapidly increasing amounts of data. In particular, the analysis of image data acquired in genome-wide cell phenotype screens constitutes a substantial bottleneck in the evaluation process and motivates the development of automated image analysis tools for large-scale experiments. In this chapter, we present a computational scheme to process multicell time-lapse images as they are produced in high-throughput screens. We describe an approach to automatically segment and classify cell nuclei into different mitotic phenotypes. This enables automated identification of cell cultures that show an abnormal mitotic behavior. Our scheme proves high classification accuracy, suggesting a promising future for automating the evaluation of high-throughput experiments
    Type of Publication: Book chapter
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...