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  • Springer  (118,612)
  • Public Library of Science (PLoS)  (10,482)
  • 2015-2019  (13,888)
  • 1970-1974  (115,206)
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  • 1
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    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 2
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 3
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 4
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 5
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 6
    Keywords: Microbiology ; Food science ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products -- Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens -- The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens -- Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection -- Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices -- Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria -- EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes -- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions -- Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation -- Isolation of Bacterial RNA from Foods Inoculated with Pathogens -- The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress -- Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics -- Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells -- High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens -- Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae -- Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection -- Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback -- The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform -- Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics -- Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments -- Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars
    Abstract: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens
    Pages: XI, 278 p. 53 illus., 43 illus. in color. : online resource.
    ISBN: 9781493990009
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  • 7
    Call number: WN180:8
    Keywords: Diagnostic Imaging ; Artificial intelligence
    Pages: xv, 373 p. : ill.
    ISBN: 9783319948775
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    WN180:8 on loan
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  • 8
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    Singapore : Springer
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Protective Responses of Different Organs to Environmental Toxicants or Stresses -- Avoidance Behaviour of Nematodes to Environmental Toxicants or Stresses -- Intestinal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Epidermal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Toxicity Induction in Intestine and Epidermis in Nematodes Exposed to Environmental Toxicants or Stresses -- Toxicity Induction in Neurons and Muscle in Nematodes Exposed to Environmental Toxicants or Stresses -- Reproductive Toxicity Induction in Nematodes Exposed to Environmental Toxicants or Stresses -- Intestinal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Epidermal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Neuronal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Germline Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses
    Abstract: This book introduces readers to intestinal and epidermal barriers, and to toxicity induction of environmental toxicants or stresses in the intestine, epidermis, neurons, muscle, and reproductive organs in Caenorhabditis elegans. In addition, it discusses the protective responses of various organs and nematodes’ avoidance behaviour with regard to environmental toxicants or stresses. The intestinal, epidermal, neuronal, and germline signalling pathways required for the regulation of toxicity of environmental toxicants or stresses are also introduced and discussed. As a classic model animal with well-described genetic and developmental backgrounds, the nematode Caenorhabditis elegans has been successfully and widely used in both toxicity assessments and toxicological studies on various environmental toxicants and stresses. Once exposure to certain environmental toxicants has occurred, the toxicants can enter into the primary targeted organs (such as intestinal cells), and even be translocated into secondary targeted organs (such as reproductive organs and neurons). Based on related available data, this book provides a systematic understanding of target organ toxicology in C. elegans
    Pages: XIII, 337 p. 232 illus., 175 illus. in color. : online resource.
    ISBN: 9789811360107
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  • 9
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 10
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    Cham : Springer
    Call number: QZ200:576 ; C100:50
    Pages: vii, 205 p. : ill.
    ISBN: 9783030012069
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    QZ200:576 available
    C100:50 departmental collection or stack – please contact the library
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  • 11
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: A Genomic View of the Catabolism of Aromatic Compounds in Pseudomonas -- A Genomic View of the Catabolism of Short Chain Alkanes -- Aerobic Degradation of Aromatic Hydrocarbons -- Aerobic Degradation of Aromatic Hydrocarbons: Enzyme Structures and Catalytic Mechanisms -- Aerobic Degradation of Chloroaromatics -- Aerobic Degradation of Gasoline Ether Oxygenates -- Aerobic Degradation of Halogenated Aliphatics -- Biochemistry and Molecular Biology of Methane Monooxygenase -- Biosynthesis, Insertion and Function of Fe-S Cofactors -- Biosynthesis, Insertion and Function of Flavin Cofactors -- Biosynthesis, Insertion and Function of haem-iron Prosthetic Groups -- Computational Framework for Integration of Lipidomics Data into Metabolic Pathways -- Degradation of Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) -- Degradation of Rubber/Polyisoprene -- Diversity and Common Principles in Enzymatic Activation of Hydrocarbons: An Introduction -- Energetic and Other Quantitative Aspects of Microbial Hydrocarbon Utilization: An Introduction: -- Enzymes for Aerobic Degradation of Alkanes in Bacteria -- Enzymes for Aerobic Degradation of Alkanes in Yeasts -- Evolution of New Catabolic Functions Through Gene Assembly by Mobile Genetic Elements -- Experimental Evolution of Novel Regulatory Activities in Response to Hydrocarbons and Related Chemicals -- Functional Gene Diversity, Biogeography, Dynamics -- Genetic Features and Regulation of n-Alkane Metabolism in Bacteria -- Genetic Features and Regulation of n-Alkane Metabolism in Yeasts -- Genetic Features of Methylotrophs -- Genetics and Functional Genomics of Aerobic Degradation of Hydrocarbons: An Introduction -- Genetics and Molecular Features of Bacterial Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) Transformations -- Genetics of Biphenyl Biodegradation and Co-Metabolism of PCBs -- Genetics of Rubber/Polyisoprene Degradation -- Genetics and Ecology of Isoprene Degradation -- Genomic View of Mycobacterial High Molecular Weight Polycyclic Aromatic Hydrocarbon Degradation -- Global Aerobic Degradation of Hydrocarbons in Aquatic Systems -- Global Aerobic Degradation of Hydrocarbons in Terrestrial Systems -- Global Consequences of the Microbial Production and Consumption of Inorganic and Organic Sulfur Compounds -- Key Features of Aerobic Hydrocarbon Biodegrader Genomes -- Lipid Degradation - Lipid Cycles -- Lipolytic Enzymes from Bacteria -- Lipolytic Enzymes from Yeasts -- Metabolism of Steroids -- Oxidative Inactivation of Ring-Cleavage Extradiol Dioxygenases: Mechanism and Ferredoxin-Mediated Reactivation -- Pathways for the Degradation of Fatty Acids in Bacteria -- Phylogenomics of Aerobic Bacterial Degradation of Aromatics -- Physiology and Biochemistry of the Aerobic Methane Oxidizing Bacteria -- Potential for Microbial Interventions to Reduce Global Warming -- Rational Construction of Bacterial Strains with New/Improved Catabolic Capabilities for the Efficient Breakdown of Environmental Pollutants -- Regulation of the Degradation of Fatty Acids in Bacteria -- Regulation of the Degradation of Fatty Acids in Yeast -- Stereochemistry of Hydrocarbon-transforming Enzyme Reactions -- Structure-Function Relationships and Engineering of Haloalkane Dehalogenases -- Transcriptional Control of the TOL Plasmid Pathways
    Abstract: This book describes the biochemistry and genetics of aerobic degradation of hydrocarbons and lipids by bacteria and yeasts, as well as the functional genomics of the best-known microorganisms involved in these processes. It forms part of the Handbook of Hydrocarbon and Lipid Microbiology Series, a definitive resource of current knowledge on the diverse aspects of the interactions of microbes with hydrocarbons and lipids. Chapters are mainly focused on the new discoveries of recent years. It is aimed to scientists and others interested in different aspects of the microbiology of hydrocarbons
    Pages: 149 illus., 43 illus. in color. eReference. : online resource.
    ISBN: 9783319504186
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  • 12
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 13
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Physiological Characterization of Phototropism in Arabidopsis Seedlings -- Assessing Negative and Positive Phototropism in Lianas -- Determination of Phototropism and Polarotropism in Fern Protonemal Cells -- Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli -- An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens -- Observation of Phototropic Responses in the Liverwort Marchantia polymorpha -- Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus -- Quantitative Analysis of Tip Growth, Phototropic Responses and Other Blue Light-Dependent Photoresponses of Vaucheria -- A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezonensis -- Determination of Phototropism by UV-B Radiation -- New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport -- Determination of Auxin Flow during Phototropic Responses Using Fluorescent Auxin Analogs -- Immunolocalization of IAA Using an Anti-IAA-C Antibody Raised against Carboxyl-Linked IAA -- Measurements of Photoreaction and Kinase Activity of Phototropin, a Photoreceptor Protein for Tropic Response in Plants; Involvement of Kinase Activity in the Photosensitivity of Tropic Response -- Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema -- Grafting as a Tool for Study of Phototropism in Phycomyces -- Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station -- How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening -- Quantitative Measurements of Curvature along the Growth Axis in Tropic Responses Using Free Software Environments
    Abstract: This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin. In addition, the use of grafting, spaceflight experiment and image analysis in tropism study is provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phototropism: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: X, 240 p. 89 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990153
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  • 14
    Keywords: Biochemistry ; Medical laboratories ; Protein Science ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Using Data Mining to Explore Calmodulin Bibliography -- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator -- High Resolution Calcium Imaging Method for Local Calcium Signaling -- Measurement of Contractility and Calcium Release in Cardiac Spheroids -- Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis -- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio) -- Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements -- Designing Calcium Binding Proteins for Molecular MR Imaging -- Coordination to Divalent Cations by Calcium Binding Proteins -- Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo -- Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets -- Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes -- Expression and Purification of Calmodulin for NMR and Other Biophysical Applications -- The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells -- Calpain Purification through Calpastatin and Calcium: Strategy and Procedures -- Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins -- Expression and Characterization of MICU2, a Ca2+ Sensor Protein -- S100 Proteins in the Innate Immune Response to Pathogens -- Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors -- Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy -- Isolation and Characterization of S100 Protein-Protein Complexes -- The Multifaceted S100A4 Protein in Cancer and Inflammation -- Interaction of S100A6 with Target Proteins In Vitro and in Living Cells -- Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7) -- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin -- Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography -- Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex -- Analysis of S100A11 in DNA Damage Repair -- Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography -- Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure -- High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins -- Calcium-Induced Protein Folding in Calumenin and Calmodulin -- Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing -- Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies -- Tryptophan Scanning Mutagenesis of EF-Hand Motifs -- Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling -- Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level -- Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation -- Characterization of Calcium-Binding Proteins from Parasitic Worms -- High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome -- Targeted Mass Spectrometry of S100 Proteins -- Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury -- Serum S100B Levels in Melanoma -- The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine -- S100A7 in Psoriasis: Immunodetection and Activation by CRISPR -- S100A8/A9 in Myocardial Infarction -- Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults -- S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions
    Abstract: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry
    Pages: XIX, 779 p. 175 illus., 117 illus. in color. : online resource.
    ISBN: 9781493990306
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  • 15
    Keywords: Neurosciences ; Stem Cells ; Laboratory animals ; Neurosciences ; Stem Cells ; Animal Models ; Springer eBooks
    Description / Table of Contents: Generation of Neural Stem Cells from Induced Pluripotent Stem Cells -- Making NSC and Neurons from Patient Derived Tissue Samples -- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury -- Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells -- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells -- In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology -- Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons -- Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene -- Single-Cell Library Preparation of iPSC-derived Neural Stem Cells -- Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells -- Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons -- Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach -- Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells -- Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation -- Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart -- Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion
    Abstract: This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIV, 239 p. 51 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990078
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  • 16
    Keywords: Medicine ; Oncology ; Molecular Medicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Amplicon-based Targeted Next Generation Sequencing of Formalin Fixed Paraffin Embedded Tissue -- Library preparation using FFPE-derived Tumor DNA for High Throughput Hybridization-based Targeted or Exome Sequencing -- Bioinformatics Basics for High Throughput Hybridization-based Targeted DNA Sequencing From FFPE-Derived Tumor Specimens: From Reads to Variants -- Annotation of Variant Data from High Throughput DNA Sequencing from Tumor Specimens: Filtering Strategies to Identify Driver Mutations -- Biological Interpretation of Complex Genomic Data -- Clinical Validation of Targeted Solid Tumor Profiling -- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors -- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation -- Overview of Fusion Detection Strategies Using Next Generation Sequencing -- Clinical Application of Fusion Gene Detection Using Next-generation Sequencing and the NanoString Technology -- Pipeline for Integrated Microarray Expression Normalization Toolkit (PIMENTo) for Tumor Microarray Profiling Experiment -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: Overview of Library Preparation Methods -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: From Raw Data to Systems Level Analyses -- Methylation Analysis using Microarrays: Analysis and Interpretation -- High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) -- A Pipeline for ctDNA Detection Following Primary Tumor Profiling Using A Cancer-related Gene Sequencing Panel -- Single-cell Analysis of Circulating Tumor Cells
    Abstract: This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field
    Pages: XI, 268 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493990047
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  • 17
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 18
    Keywords: Biomedical Engineering ; Biology / Data processing ; Biotechnology ; Biomedical Engineering/Biotechnology ; Computer Appl. in Life Sciences ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Enhancing Nursing Education through Affordable and Realistic Holographic Mixed Reality: The Virtual Standardized Patient for Clinical Simulation -- Potential Application Of Virtual Reality For Interface Customisation (And Pre-Training) Of Amputee Patients As Preparation For Prosthetic Use -- Visualising Medical Heritage: New Approaches To Digitisation And Interpretation Of Medical Heritage Collections -- Integrating 3D visualisation technologies in undergraduate anatomy education -- Pedagogical perspectives on the use of technology within medical curricula: moving away from norm driven implementation -- Towards a More User-Friendly Medication Information Delivery to People Living with Multiple Sclerosis: A Case Study with Alemtuzumab -- The Co-Design of Hand Rehabilitation Exercises for Multiple Sclerosis Using Hand Tracking System -- Examining Vascular Structure And Function Using Confocal Microscopy And 3D Imaging Techniques -- Which Tool Is Best: 3D Scanning Or Photogrammetry - It Depends On The Task -- Application of Photogrammetry in Biomedical Science
    Abstract: This edited volume explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in imaging and analysis, education, engagement and training. The reader will be able to explore the utilisation of technologies from a number of fields to enable an engaging and meaningful visual representation of the life sciences. This use of technology-enhanced learning will be of benefit for the learner, trainer, in patient care and the wider field of education and engagement. By examining a range of techniques in image capture (photogrammetery, stereophotogrammetry, microphotogrammetry and autostereoscopy), this book will showcase the wide range of tools we can use. Researchers in this field will be able to find something suitable to apply to their work to enhance user engagement through improved visual means using the technologies we have available to us today. It will highlight the uses of these technologies to examine many aspects of the human body, and enable improved ways to enhance visual and tactile learning, including 3D printing. By demonstrating co-design processes, working directly with the end-stage users (including patients), it will also highlight successes in adopting tools like hand motion tracking rehabilitation for patients with conditions like multiple sclerosis. The book will also discuss the applications of immersive environments including virtual, augmented and mixed reality. The ultimate aim is to show how, by using these tools, we can enhance communication, mobile applications, health literacy and illustration of both normal and pathological processes in the body. By applying a wide range of tools and technologies, this volume will highlight the wide range of applications in education, training and learning both for students and faculty, but also for patient care and education. Therefore, the work presented here can be accessed by a wide range of users from faculty and students involved in the design and development of these processes, by examining the pedagogy around these technologies. Importantly, it presents material, which will be of benefit for the patient, engaging them to become more involved with techniques like physiotherapy
    Pages: XV, 132 p. 65 illus., 42 illus. in color. : online resource.
    ISBN: 9783030060701
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  • 19
    Keywords: Microbiology ; Microbial genetics ; Microbial genomics ; Biomedical Engineering ; Eukaryotic Microbiology ; Microbial Genetics and Genomics ; Applied Microbiology ; Medical Microbiology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Industrial Applications and Model Systems -- Physiological genomics of multistress resistance in the yeast cell model and factory: focus on MDR/MXR transporters -- Mechanisms of yeast adaptation to wine fermentations -- Development of robust yeast strains for lignocellulosic bio-refineries based on genome-wide studies -- Physiological genomics of the highly weak-acid-tolerant food spoilage yeasts of Zygosaccharomyces bailii sensu lato -- Yeast genome-scale metabolic models for simulating genotype-phenotype relations -- Human Health and Disease -- Emerging mechanisms of drug resistance in Candida albicans -- Genome-wide response to drugs and stress in the pathogenic yeast Candida glabrata -- Lipidomics approaches: applied to the study of pathogenesis in Candida species -- Yeast at the forefront of research on ageing and age-related diseases
    Abstract: This book discusses genome-based strategies to provide a holistic understanding of yeasts in Human Health and as model organisms in basic research or industrial production. Using numerous Saccharomyces cerevisiae strains and various non-conventional yeast species isolated from diverse origins, it describes essential biological processes, the biotechnological exploitation of yeast and pathogenesis control. It also demonstrates how functional and comparative genomics and the development of genome engineering tools are used in modern yeast research. The use of yeasts as experimental eukaryotic models increasingly gained prominence when several Nobel Prizes in Physiology/Medicine and Chemistry were awarded for innovative research, using yeast strains to elucidate molecular mechanisms in a wide range of human physiological processes and diseases, such as autophagy, cell cycle regulation and telomerase activity. This book offers useful insights for scientists in yeast research, clinical scientists working with yeast infectious models and for industrial researchers using applied microbiology
    Pages: V, 242 p. 37 illus., 29 illus. in color. : online resource.
    ISBN: 9783030130350
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  • 20
    Keywords: Entomology ; Plant breeding ; Plant anatomy ; Biomedical Engineering ; Mycology ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Preface -- Biodiversity of Fungi from Extreme Habitats and Its Industrial Applications -- Rhizospheric Fungi Isolated from Different Plants and Potential Applications in Diverse Sectors -- Endophytic Fungi from Different Niches with Potential Industrial Importance -- Marine Fungal White Biotechnology -- Aspergillus Biodiversity, Ecological Significance, and Industrial Applications -- Fusarium Biodiversity, Ecological Significance, and Industrial Applications -- Penicillium Biodiversity, Ecological Significance, and Industrial Applications -- Trichoderma Biodiversity, Ecological Significance, and Industrial Applications -- Thermostable Enzymes from Fungi Isolated from Hot Springs and Its Applications -- Cold Active Enzymes from Psychrophilic Fungi and Its Applications -- Industrially Important Enzymes from White-Rot Fungi or Filamentous Fungi -- Fungal Enzymes for the Textile Industry -- Fungal Laccase -- Fermentation Process for Production of Fungal Cellulases -- Fungal Lipases -- Fungal Community and Eco-Friendly Bioresources for Novel Secondary Metabolites -- Agriculturally Important Fungi -- Fungal White Biotechnology in Biosurfactants -- Synthetic Biology and a Novel Approach for Pharmaceutically Important Compounds -- Global Scenario of Fungal White Biotechnology -- Appendixes -- Index --
    Abstract: White biotechnology, or industrial biotechnology as it is also known, refers to the use of living cells and/or their enzymes to create industrial products that are more easily degradable, require less energy, create less waste during production and sometimes perform better than products created using traditional chemical processes. Over the last decade considerable progress has been made in white biotechnology research, and further major scientific and technological breakthroughs are expected in the future. Fungi are ubiquitous in nature and have been sorted out from different habitats, including extreme environments (high temperature, low temperature, salinity and pH), and may be associated with plants (epiphytic, endophytic and rhizospheric). The fungal strains are beneficial as well as harmful for human beings. The beneficial fungal strains may play important roles in the agricultural, industrial, and medical sectors. The fungal strains and their products (enzymes, bioactive compounds, and secondary metabolites) are very useful for industry (e.g., the discovery of penicillin from Penicillium chrysogenum). This discovery was a milestone in the development of white biotechnology as the industrial production of penicillin and antibiotics using fungi moved industrial biotechnology into the modern era, transforming it into a global industrial technology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors, providing both high value nutraceutical and pharmaceutical products. The fungal strains and bioactive compounds also play an important role in environmental cleaning. This volume covers the latest developments and research in white biotechnology with a focus on diversity and enzymes
    Pages: XIX, 571 p. 108 illus., 61 illus. in color. : online resource.
    ISBN: 9783030104801
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  • 21
    Keywords: Medicine ; Mathematical Statistics ; Biomedicine general ; Statistics and Computing/Statistics Programs ; Springer eBooks
    Description / Table of Contents: Preface -- General Introduction -- Significant and Insignificant Adverse Effect -- Incidence Ratios and Reporting Ratios of Adverse Effects -- Safety Analysis and the Alternative Hypothesis -- Forest Plots of Adverse Effects -- Graphics of Adverse Effects -- Repeated Measures Methods for Testing Adverse Effects -- Benefit Risk Ratios -- Equivalence, Non-inferiority and Superiority Testing of Adverse Effects -- Part II The Analysis of Dependent Adverse Effects -- Independent and Dependent Adverse Effects. Categorical Predictors Assessed as Dependent Adverse Effects. Adverse Effect of the Dependent Type in Crossover Trial -- Confoundings and Interactions Assessed as Dependent Adverse Effects -- Subgroup Characteristics Assessed as Dependent Adverse Effects -- Random Effects Assessed as Dependent Adverse Effects -- Outliers Assessed as Dependent Adverse Effects -- Index
    Abstract: The authors, as professors in statistics at various universities in Europe, are worrried about the poor quality of safety data analysis of clinical trials, despite its importance in drug development and pharmacovigilance. Clinical trials, not adequately addressing safety, are unethical. An effective approach for the purpose is to present summaries of prevalences. In order to estimate the probability, that the differences between treatment and control group did not occur merely by chance, a statistical test can be performed. This pretty crude method has recently be supplemented with better sensitive methodologies, based on machine learning clusters and networks, and multivariate analyses. Another important novelty with safety data analysis is the new insights into hypothesis testing, favoring the alternative hypotheses instead of the null hypotheses. Finally the issue of dependency is addressed. Adverse effects may be either dependent or independent of the main outcome. Dependent adverse effect are dependent not only on the treatment modalities, but also on the outcome of the trials. Random heterogeneities, outliers, confounders, interaction factors are common in clinical trials, and all of them can be considered kinds of adverse effects of the dependent type. Random regressions and analyses of variance, high dimensional clusterings, partial correlations, structural equations models, and other Bayesian methods are helpful for their analysis. The current edition was particularly written for medical and health professionals and students. It provides examples of modern analytic methods so far largely unused. All of the 16 chapters have two core characteristics, first they are for current usage, second they try and tell what readers need to know in order to understand the methods. Step by step analyses are given and self-assessment examples are supplied. Each chapter can be studied as a stand-alone
    Pages: XI, 217 p. 191 illus., 28 illus. in color. : online resource.
    ISBN: 9783030058043
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  • 22
    Keywords: Human Genetics ; Medical virology ; Human Genetics ; Virology ; Springer eBooks
    Description / Table of Contents: 1. Design of AAV vectors for Delivery of RNAi -- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes -- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer -- 4. Quantitative and Digital Droplet-based AAV Genome Titration -- 5. Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual -- 6. In Situ Hybridization for Detection of AAV-Mediated Gene Expression -- 7. Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina -- 8. Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System -- 9. Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-Transgenic Species -- 10. MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain -- 11. AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection -- 12. Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals -- 13. Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia -- 14. SubILM Injection of AAV for Gene Delivery to the Retina -- 15. Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins -- 16. AAV-Mediated Gene Delivery to the Inner Ear -- 17. Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System -- 18. AAV-Mediated Gene Delivery to Taste Cells of the Tongue -- 19. AAV Vectors for Efficient Gene Delivery to Rodent Hearts -- 20. AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods -- 21 -- AAV-Mediated Gene Delivery to the Lung -- 22. Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration -- 23. rAAV-Mediated Gene Delivery to Adipose Tissue -- 24. AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection
    Abstract: This volume provides a complete and timely guide to the use of adeno-associated virus (AAV) vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease
    Pages: XII, 426 p. 62 illus., 50 illus. in color. : online resource.
    ISBN: 9781493991396
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  • 23
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    Cham : Springer
    Keywords: Human Genetics ; Developmental Biology ; Immunology ; Medical virology ; Human Genetics ; Developmental Biology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Part 1 Foundations for muscle gene therapy -- Chapter 1. An overview of muscle biology and physiology for muscle gene therapy -- Chapter 2. Molecular basis of muscle disease -- Chapter 3. Animal models for muscle disease and muscle gene therapy -- Chapter 4. Muscle stem cell biology and implication in gene therapy -- Chapter 5. Pluripotent stem cells for gene therapy of hereditary muscle disorders -- Chapter 6. MicroRNAs (miRs) in muscle gene therapy -- Chapter 7. Immune system regulation of muscle injury and disease -- Chapter 8. Design of muscle gene therapy expression cassette -- Chapter 9 Non-viral vector mediated muscle gene therapy -- Chapter 10. Viral vectors for muscle gene therapy -- Chapter 11. Development of next generation muscle gene therapy AAV vectors -- Chapter 12. Histological and biochemical evaluation of muscle gene therapy -- Chapter 13. Optical polarization tractography imaging of structural changes in the skeletal and cardiac muscles of the mdx4cv mice -- Chapter 14. Biomarkers for muscle disease gene therapy -- Chapter 15. Large-scale clinical manufacturing of AAV Vectors for systemic muscle gene therapy -- Chapter 16. Genome-editing for muscle gene therapy -- Part 2 Preclinical muscle gene therapy -- Chapter 17. Considerations on preclinical muscle gene therapy studies -- Chapter 18. Gene replacement therapy for Duchenne muscular dystrophy -- Chapter 19. Recent advances in AON-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 20. AAV-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 21. Alternate translational initiation of dystrophin: a novel therapeutic approach -- Chapter 22. Genome editing for Duchenne muscular dystrophy -- Chapter 23. Sarcolipin knockdown therapy for Duchenne muscular dystrophy -- Chapter 24. Gene therapy for central nervous system in Duchenne muscular dystrophy -- Chapter 25. Therapeutic approach for dysferlinopathy in animal models -- Chapter 26. Muscle cell membrane repair and therapeutic implications -- Chapter 27. Treatment of muscular dystrophy-dystroglycanopathy: unlocking the potential of genetic engineering -- Chapter 28. RNAi therapy for dominant muscular dystrophies and other myopathies -- Chapter 29. Gene therapy for facioscapulohumeral muscular dystrophy (FSHD) -- Chapter 30. Gene therapy and gene editing for myotonic dystrophy -- Chapter 31. Gene therapy for oculopharyngeal muscular dystrophy -- Chapter 32. Gene therapy for X-linked myotubular myopathy -- Chapter 33. Pre-clinical gene therapy studies for metabolic myopathy -- Chapter 34 Elimination of mutant mitochondrial DNA in mitochondrial myopathies using gene editing enzymes -- Chapter 35. Gene therapy for Charcot-Marie-Tooth inherited neuropathy -- Chapter 36. Muscle - a potent target in vaccination -- Part 3 Clinical muscle gene therapy -- Chapter 37. Patient and family perspective on muscle gene therapy -- Chapter 38. Design of clinical trials for gene therapy in muscular dystrophy -- Chapter 39. Path to clinical trials: trial design, development of the clinical product and safety concerns in the implementation of clinical trials -- Chapter 40. Muscle MRI as an endpoint in clinical trials -- Chapter 41. Gene therapy clinical trials for Duchenne and limb girdle muscular dystrophies lessons learned -- Chapter 42. Duchenne muscular dystrophy exon-skipping trials -- Chapter 43. What we have learned from 10 years of DMD exon skipping trials -- Chapter 44. Clinical gene therapy trials for Pompe disease -- Chapter 45. Muscle-directed gene therapy for alpha-1 antitrypsin deficiency
    Abstract: About 7 million people worldwide are suffering from various inherited neuromuscular diseases. Gene therapy brings the hope of treating these diseases at their genetic roots. Muscle Gene Therapy is the only book dedicated to this topic. The first edition was published in 2010 when the field was just about to enter its prime time. The progress made since then has been unprecedented. The number of diseases that have been targeted by gene therapy has increased tremendously. The gene therapy toolbox is expanded greatly with many creative novel strategies (such as genome editing and therapy with disease-modifying genes). Most importantly, clinical benefits have begun to emerge in human patients. To reflect rapid advances in the field, we have compiled the second edition of Muscle Gene Therapy with contributions from experts that have conducted gene therapy studies either in animal models and/or in human patients. The new edition offers a much needed, up-to-date overview and perspective on the foundation and current status of neuromuscular disease gene therapy. It provides a framework to the development and regulatory approval of muscle gene therapy drugs in the upcoming years. This book is a must-have for anyone who is interested in neuromuscular disease gene therapy including those in the research arena (established investigators and trainees in the fields of clinical practice, veterinary medicine and basic biomedical sciences), funding and regulatory agencies, and patient community
    Pages: XVI, 811 p. 78 illus., 58 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030030957
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  • 24
    Keywords: Biomedical Engineering ; Computer engineering ; Biomedical Engineering/Biotechnology ; Biomedical Engineering and Bioengineering ; Signal, Image and Speech Processing ; Biological and Medical Physics, Biophysics ; Electrical Engineering ; Springer eBooks
    Description / Table of Contents: Part I Signal Processing for Physiology -- Introduction to Measurement and Analysis in Physiology -- Basic Concepts in Systems Theory and Signal Processing -- Signal Filtering and System Control for Physiology -- Digitization and Discrete Systems - Sampling and Quantization -- Discrete Signal Processing for Physiological Signals -- Graphics and Haptics for Simulation Models -- Model Based Analysis of Physiological Systems -- Part II Models of Physiological Systems -- Nerve Communication I - Wave Propagation and Action Potentials -- Neural Communication II - Firing Rate and Information Encoding -- External Stimulation of Nerves in Tissue - From Theory to Practice -- Skeletal Muscle Contraction - Force and Movement -- The Electromyogram - Modeling and Analysis -- Neuromuscular Control - Spinal Reflex and Movement -- Cardiovascular Blood Flow Modeling -- Immune Response to Infection
    Abstract: Physiology is a set of processes that maintain homeostasis, and physiological measurement is a means of observing these processes. Systems theory and signal processing offer formal tools for the study of processes and measured quantities. This book shows that systems modeling can be used to develop simulations of physiological systems, which use formal relations between the underlying processes and the observed measurements. The inverse of such relations suggest signal processing tools that can be applied to interpret experimental data. Both signal processing and systems modeling are invaluable in the study of human physiology. Discussing signal processing techniques ranging from filtering and spectrum analysis to wavelet analysis, the book also includesGraphs and analogies to supplement the mathematics and make the book more accessible to physiologists and also more interesting to engineers. Physiological systems modeling helps in both gaining insights and generating methods of analysis. This book shows how numerical computation with graphical display, haptics and multimedia can be used to simulate physiological systems. In this third edition the simulations are more closely related to clinical examination and experimental physiology than in previous editions. Detailed models of nerve and muscle at the cellular and systemic levels, and simplified models of cardiovascular blood flow provide examples for the mathematical methods and computer simulations. Several of the models are sufficiently sophisticated to be of value in understanding real world issues like neuromuscular disease. The book features expanded problem sets and a link to extra downloadable material, and simulation programs that are solutions to the theory developed in the text are also available
    Pages: XV, 468 p. 362 illus., 123 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9789811335310
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  • 25
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    New York, NY : Springer
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: Nonsurgical Hammertoe Treatment Options -- PIPJ Arthroplasty without Fixation -- PIPJ Interpositional Arthroplasty -- PIPJ Arthroplasty with Temporary Kirchner Wire Fixation -- PIPJ Arthrodesis, Peg and Hole -- PIPJ Arthrodesis via Buried Kirchner Wire -- PIPJ Arthrodesis with a 1 Component Metallic Implant -- PIPJ Arthrodesis with a 2 Component Metallic Implant -- PIPJ Arthrodesis with a Thermal Activated Metallic Implant -- PIPJ Arthrodesis with an Allograft Implant -- PIPJ Arthrodesis with a Bioabsorbable Implant -- Flexor Tendon Transfer -- Tenotomies -- MTP Joint: Soft Tissue Balancing (skin plasties and lengthenings, MTPJ release, extensor tendon lengthening, capsulotomy, capsulorraphy) -- MTP Joint: Plantar Plate Reconstruction via a Dorsal Approach -- MTP Joint: Plantar Plate Repair via a Plantar Approach -- Lesser Metatarsal Osteotomies: uniplanar, biplanar wedge osteotomies -- MTP Joint: Transverse Plane Correction via Collateral Ligament Repair -- MTP Joint Transverse Plane Correction via Metatarsal Osteotomy -- Crossover Toe Correction -- Congenital Overlapping Fifth Toe -- Flail Hammertoe Correction
    Abstract: Comprised exclusively of clinical cases demonstrating the various management strategies for hammertoes, this concise, practical casebook will provide foot and ankle surgeons with the best real-world strategies to properly treat these frequent deformities of the lower extremity and their complications. Beginning with a review of the relevant anatomy and biomechanics of the toe, each subsequent chapter includes case material with a unique clinical presentation, followed by a description of the diagnosis, assessment and management techniques used to treat it, as well as the case outcome, literature review and clinical pearls and pitfalls. Chapters included illustrate different surgical fixation techniques, including PIPJ arthroplasty and arthrodesis, tendon transfers and tenotomies, and osteotomies, as well as management strategies for complications. Pragmatic and reader-friendly, Hammertoes: A Case-Based Approach is an excellent resource for foot and ankle surgeons treating this common but frustrating condition
    Pages: XIII, 419 p. 511 illus., 427 illus. in color. : online resource.
    ISBN: 9783319165523
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  • 26
    Keywords: Biochemistry ; Bioinformatics ; Protein Structure ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 55 Years of the Rossmann Fold -- Advances in Protein Super-Secondary Structure Prediction and Application to Protein Structure Prediction -- Automated Family-Wide Annotation of Secondary Structure Elements -- Computational Prediction of Secondary and Supersecondary Structures from Protein Sequences -- StackSSSPred: A Stacking Based Prediction of Supersecondary Structure from Sequence -- Information-Theoretic Inference of an Optimal Dictionary of Protein Supersecondary Structures -- Secondary and Supersecondary Structure of Proteins as Coupling between Local and Local and Backbone-Electrostatic Interactions: A View through Cluster-Cumulant Scope -- Learning Organizations of Protein Energy Landscapes: An Application on Decoy Selection in Template-Free Protein Structure Prediction -- Exploring Protein Supersecondary Structure through Changes in Protein Folding, Stability, and Flexibility -- Protodomains: Symmetry Related Supersecondary Structures in Proteins and Self-Complementarity -- bab Super Secondary Motifs: Sequence, Structural Overview and Pursuit of Potential Autonomously Folding bab Sequences from (b/a)8/TIM Barrels -- Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for their Prediction and Characterization -- Stable Sub-Structures in Proteins and How to Find Them using Single-Molecule Force Spectroscopy -- Supersecondary Structures and Fragment Libraries -- Molecular Dynamics Simulations of Conformational Conversions in Transformer Proteins -- Sequence Pattern for Supersecondary Structure of Sandwich-Like Proteins -- Homology Searches using Supersecondary Structure Code -- Beyond Supersecondary Structure: Physics-Based Sequence Alignment -- Secondary and Supersecondary Structure of Proteins in Light of the Structure of Hydrophobic Cores -- Hands On: Using Tryptophan Fluorescence Spectroscopy to Study Protein Structure -- Structural Characterization of Membrane Protein Dimers
    Abstract: This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation
    Pages: XVIII, 438 p. 119 illus., 103 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991617
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  • 27
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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  • 28
    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 29
    Keywords: Microbiology ; Emerging infectious diseases ; Medicine ; Medical Microbiology ; Infectious Diseases ; Health Promotion and Disease Prevention ; Springer eBooks
    Description / Table of Contents: Buruli Ulcer: History and Disease Burden -- Buruli Ulcer in Africa -- Buruli Ulcer in Australia -- Mycobacterium Ulcerans Infection in French Guiana; Current State of Knowledge -- Buruli Ulcer in Japan -- Population Genomics and Molecular Epidemiology of Mycobacterium Ulcerans -- Mycolactone: More Than Just a Cytotoxin -- The Immunology of Buruli Ulcer -- Buruli Ulcer in Animals and Experimental Infection Models -- Laboratory Diagnosis of Buruli Ulcer: Challenges and Future Perspectives -- Antimicrobial Treatment of Mycobacterium Ulcerans Infection -- Thermotherapy of Buruli Ulcer -- Secondary Infection of Buruli Ulcer Lesions -- Management of BU-HIV Co-infection -- Social Science Contributions to BU Focused Health Service Research in West-Africa -- Transdisciplinary Research and Action to Stop Buruli Ulcer– A Case Study from Philanthropy
    Abstract: A major objective of this open access book is to summarize the current status of Buruli Ulcer (BU) research for the first time. It will identify gaps in our knowledge, stimulate research and support control of the disease by providing insight into approaches for surveillance, diagnosis, and treatment of Buruli Ulcer. Book chapters will cover the history, epidemiology diagnosis, treatment and disease burden of BU and provide insight into the microbiology, genomics, transmission and virulence of Mycobacterium ulcerans
    Pages: X, 287 p. 34 illus., 30 illus. in color. : online resource.
    ISBN: 9783030111144
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  • 30
    facet.materialart.
    Singapore : Springer
    Keywords: Bioinformatics ; Medicine ; Statistics ; Bioinformatics ; Biomedicine general ; Statistics for Life Sciences, Medicine, Health Sciences ; Springer eBooks
    Description / Table of Contents: Part 1. BIOINFORMATICS FOR LIFE AND PERSONAL GENOME INTERPRETATION -- Chapter 1. Bioinformatics For Life -- Chapter 2. Next Generation Sequencing and Personal Genome Data Analysis -- Chapter 3. Personal Genome Data Analysis -- Chapter 4. Personal Genome Interpretation and Disease Risk Prediction -- Part 2. ADVANCED MICROARRAY DATA ANALYSIS -- Chapter 5. Advanced Microarray Data Analysis -- Chapter 6. Gene Expression Data Analysis -- Chapter 7. Gene Ontology and Biological Pathway-based Analysis -- Chapter 8. Gene-set Approaches and Prognostic Subgroup Prediction -- Chapter 9. MicroRNA Data Analysis -- Part 3. NETWORK BIOLOGY, SEQUENCE, PATHWAY AND ONTOLOGY INFORMATICS -- Chapter 10. Network Biology, Sequence, Pathway and Ontology Informatics -- Chapter 11. Motif and Regulatory Sequence Analysis -- Chapter 12. Molecular Pathways and Gene Ontology -- Chapter 13. Biological Network Analysis -- Part 4. SNPS, GWAS AND CNVS, INFORMATICS FOR GENOME VARIANTS -- Chapter 14. SNPs, GWAS, CNVs: Informatics for Human Genome Variations -- Chapter 15. SNP Data Analysis -- Chapter 16. GWAS Data Analysis -- Chapter 17. CNV Data Analysis -- Part 5. METAGENOME AND EPIGENOME, BASIC DATA ANALYSIS -- Chapter 18. Metagenome and Epigenome Data Analysis -- Chapter 19. Metagenome Data Analysis -- Chapter 20. Epigenome Databases and Tools -- Chapter 21. Epigenome Data Analysis -- Appendix A. BASIC PRACTICE USING R FOR DATA ANALYSIS -- Appendix B. APPLICATION PROGRAM FOR GENOME DATA ANALYSIS INSTALL GUIDE
    Abstract: This textbook describes recent advances in genomics and bioinformatics and provides numerous examples of genome data analysis that illustrate its relevance to real world problems and will improve the reader’s bioinformatics skills. Basic data preprocessing with normalization and filtering, primary pattern analysis, and machine learning algorithms using R and Python are demonstrated for gene-expression microarrays, genotyping microarrays, next-generation sequencing data, epigenomic data, and biological network and semantic analyses. In addition, detailed attention is devoted to integrative genomic data analysis, including multivariate data projection, gene-metabolic pathway mapping, automated biomolecular annotation, text mining of factual and literature databases, and integrated management of biomolecular databases. This textbook is primarily intended for life scientists, medical scientists, statisticians, data processing researchers, engineers, and other beginners in bioinformatics who are experiencing difficulty in approaching the field. However, it will also serve as a simple guideline for experts unfamiliar with the new, developing subfield of genomic analysis within bioinformatics
    Pages: XVI, 367 p. 645 illus., 236 illus. in color. : online resource.
    ISBN: 9789811319426
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  • 31
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Setting Up a Single-Cell Genomic Laboratory -- Tissue Handling and Dissociation for Single-Cell RNA-seq -- Full-Length Single-Cell RNA-Sequencing with Smart-seq2 -- CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification -- Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq) -- Single-Cell RNA Sequencing with Drop-Seq -- Chromium 10X Single Cell 3’ mRNA Sequencing of Tumor Infiltrating Lymphocytes -- Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-Sequencing -- Single-Cell Tagged Reverse Transcription (STRT-seq) -- Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ -- High Throughput Single Cell Real-Time Quantitative PCR Analysis -- Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ System -- Targeted TCR Amplification from Single-Cell cDNA Libraries -- Sequencing the Genomes of Single Cells -- Studying DNA Methylation in Single Cell Format with scBS-seq -- Single Cell 5fC Sequencing -- ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions -- Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry -- Classification of the Immune Composition in the Tumor Infiltrate -- Combined Genome and Transcriptome (G&T) Sequencing of Single Cells -- Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell -- Simultaneous Targeted Detection of Proteins and RNAs in Single Cells -- CRISPR-Screening in Single Cells -- Single Cell Live Imaging -- Differential Expression Analysis in Single Cell Transcriptomics -- A Bioinformatic Toolkit for Single-Cell mRNA Analysis
    Abstract: This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab
    Pages: XVI, 459 p. 87 illus., 78 illus. in color. : online resource.
    ISBN: 9781493992409
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  • 32
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains -- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta -- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA -- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions -- Quantification of Extracellular ATP in Plant Suspension Cell Cultures -- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions -- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato -- Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIM -- Analysis of DNA Methylation Profile in Plants by Chop-PCR -- Change in Nucleosome Dynamics during Stress Responses in Plants -- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry -- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments -- Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae -- Quantification of Cauline Leaf Abscission in Response to Plant Pathogens -- Methods for Replicating Leaf Vibrations Induced by Insect Herbivores -- Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes -- The Apple Fruitlet Model System for Fire Blight Disease -- Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins -- Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems -- Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants -- Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth -- A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance
    Abstract: Back Cover Copy The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field
    Pages: XIII, 260 p. 59 illus., 42 illus. in color. : online resource.
    ISBN: 9781493994588
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  • 33
    Keywords: Neurosciences ; Neurobiology ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- What Would Happen if Human Lives Beyond Time? -- Neurophysiology of Visual Perception -- Biophysics of Vision -- Cortex, Insula and Interoception -- Interoceptive dysfunction -- The Proprioceptive System -- Immunoperception -- The Immunoperceptive System -- Extrasensory Perception: Concept, Types and Interpretation -- Extrasensory Perception: Concept and History -- Psychological Perspective on Extrasensory Perception -- Environmental Sensitivity to Extra-Sensory Stimulations and Human Evolution -- A Perspective on the Concept and Nature of the Sixth Sense -- The Sixth Sense: Is It a Chance? -- Fuzzy Sets: Application to the Sixth Sense -- Asymmetry: Extra Sparkle to the Sixth Sense? -- Synchronization Side of the Sixth Sense Story -- Follow Aura and Find the Sixth Sense -- An Evolutionary Perspective of the Sixth Sense -- The Sixth Sense: Symphony of Spooky Actions -- The Sixth Sense Organs: The Immune System -- The Sixth Sense Organs: The Heart -- The Sixth Sense Organs: The Gut -- The Sixth Sense Organs: The Eyes -- The Sixth Sense Organs: The Ears -- The Sixth Sense Organs: The Hands -- Stem Cells Have More Than Five Senses -- Learning The Sixth Sense -- Neurocircuits Underlying Intuitive Processing -- Gut Feelings in Practice -- The Manager’s Sixth Sense: An Art in Organizational, Educational, Moral, and Expert Thinking
    Abstract: The book does not aim to constitute definite edges of the sixth sense, but it is to say here that the sixth sense is the result of a break in our present property. The present property is produced from integration of five common senses by the neuro-immuno-endocrine system. Thereby, mental representations of multisensory integration that primarily occur in the brain can reach throughout the body, including the heart and gut. Unconscious thought through synchronization between spatially distant brain networks would help break our present property. It would be a felicific calculus for society people to peek around the forgotten, but real life, corners through which the book “Biophysics and Neurophysiology of the Sixth Sense” seeks to explore
    Pages: XI, 362 p. 13 illus., 3 illus. in color. : online resource.
    ISBN: 9783030106201
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  • 34
    Keywords: Toxicology ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Complexity of Biologics CMC Regulation -- Biopharmaceutics are Not Chemical Drugs -- An Effective CMC Strategy is Possible -- Challenge of Adventitious Agent Control -- Biopharmaceutical Source Materials -- Manufacturing of Biopharmaceutical APIs -- Manufacturing of the Drug Product -- Complex Process-Related Impurity Profiles -- Product Characterization is a Journey -- Priceless Potency (Therapeutic Activity) -- Quality Attributes of a Biopharmaceutical -- Designing the Stability Program -- The Art of Setting Specifications -- Demonstrating Product Comparability After Process Changes -- Invaluable CMC-Focused Meetings with Regulatory Authorities
    Abstract: This book since first published in 2004 has been a major resource providing insights and practical guidance for the CMC teams to develop an acceptable cost-effective, risk-based CMC regulatory compliance strategy for biopharmaceuticals (recombinant proteins, monoclonal antibodies, genetically engineered viruses and genetically engineered human cells) from early clinical stage development through market approval. The third edition of this book provides added coverage for the biosimilars, antibody drug conjugates (ADCs), bispecific antibodies, genetically engineered viruses, and genetically engineered cells. This third edition of the book also addresses the heightened pressure on CMC regulatory compliance timelines due to the introduction of expedited clinical pathways moving the clinical development closer to a seamless phase process (e.g., FDA Breakthrough Therapy designation, CBER Regenerative Medicine Advanced Therapy (RMAT) designation, EMA Priority Medicines (PRIME) designation). The Challenge of CMC Regulatory Compliance for Biopharmaceuticals is essential, practical information for all pharmaceutical development scientists, Manufacturing and Quality Unit staff, Regulatory Affairs personnel, and senior management involved in the manufacture of biopharmaceuticals
    Pages: XXVIII, 426 p. 59 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9783030137540
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  • 35
    Keywords: Biochemistry ; Gene Expression ; Post-translational modification ; Protein Science ; Gene Expression ; Posttranslational Modification ; Springer eBooks
    Description / Table of Contents: High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets -- Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry -- Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty -- Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC -- Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo -- Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments -- Lysine Acetylation of Proteins and its Characterization in Human Systems -- Molecular and Cellular Characterization of SIRT1 Allosteric Activators -- Assessment of SIRT2 Inhibitors in Mouse Models of Cancer -- Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair -- Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes -- Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation -- Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons -- Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability -- Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling -- Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics -- Functional Analysis of HDACs in Tumorigeneis
    Abstract: The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems
    Pages: XIII, 310 p. 52 illus., 40 illus. in color. : online resource.
    ISBN: 9781493994342
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  • 36
    Keywords: Developmental Biology ; Cytology ; Developmental Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The neural crest: a remarkable model system for studying development and disease -- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example -- Generation of neural crest progenitors from human pluripotent stem cells -- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling -- Deciphering the neural crest contribution to cephalic development with avian embryos -- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis -- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants -- Technologies for live imaging of enteric neural crest derived cells -- Mouse embryo culture for the study of neural crest cells -- Laser capture microdissection of murine embryonic neural crest cells -- In vivo and in vitro quantitative analysis of neural crest cell migration -- Proteomics in neural crest cell research -- Neural crest methodologies in zebrafish and medaka -- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells -- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity -- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective
    Abstract: This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike
    Pages: X, 230 p. 36 illus. in color. : online resource.
    ISBN: 9781493994120
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  • 37
    Keywords: Medicine ; Pharmaceutical technology ; Cardiovascular System ; Molecular Medicine ; Pharmaceutical Sciences/Technology ; Cardiovascular Biology ; Springer eBooks
    Description / Table of Contents: Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview -- Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry -- Analysis of Vascular Hydrogen Sulfide Biosynthesis -- Measurement of Protein Persulfidation: Improved Tag-Switch Method -- ProPerDP: A Protein Persulfide Detection Protocol -- Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells -- In Vitro Measurement of H2S-Mediated Vasoactive Responses -- In Vivo Measurement of H2S, Polysulfides, and ‘SSNO-mix’-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform -- Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries -- The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum -- Pharmacological Tools for the Study of H2S Contribution to Angiogenesis -- Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats -- Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats -- Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity -- Vascular Myography to Examine Functional Responses of Isolated Blood Vessels
    Abstract: This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study
    Pages: X, 221 p. 70 illus., 47 illus. in color. : online resource.
    ISBN: 9781493995288
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  • 38
    Keywords: Pharmaceutical technology ; Nanotechnology ; Pharmaceutical Sciences/Technology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Nanovesicles for Nanomedicine: Theory and Practices -- Preparation and Characterization of Micelles -- Anionic and Cationic Vitamin E-TPGS Mixed Polymeric Phospholipid Micellar Vehicles -- Phospholipid Micelles for Peptide Drug Delivery -- Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery -- Gelatin Nanoparticles -- Green Synthesis of Chitosan-Silver/Gold Hybrid Nanoparticles for Biomedical Applications -- Methods of Fabrication of Chitosan-Based Nano-in-Microparticles (NMPs) -- Fabrication of Mucoadhesive-Dendrimers as Solid Dosage Forms -- In Situ Vaccination of Tumors Using Plant Viral Nanoparticles -- Bioconjugation in Drug Delivery: Practical Perspectives and Future Perceptions -- Conjugation of Triphenylphosphonium Cation to Hydrophobic Moieties to Prepare Mitochondria-Targeting Nanocarriers -- Surface Modification of Biomedically Essential Nanoparticles Employing Polymer Coating -- Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery -- Preparation of Responsive Carbon Dots for Anticancer Drug Delivery -- Surface Modification of Nanoparticles and Nanovesicles via Click Chemistry -- Polymersomes: Preparation and Characterization -- Partially Polymerized Phospholipid Vesicles for Efficient Delivery of Macromolecules -- Fabrication of Nanostructured Lipid Carriers (NLC)-Based Gels from Microemulsion Template for Delivery through Skin -- Preparation and Characterization of Solid Lipid Nanoparticles-Based Gel for Topical Delivery -- Molecular-Level €œÓbservationś€ of the Behavior of Gold Nanoparticles in Aqueous Solution and Interacting with a Lipid Bilayer Membrane -- Atomic Force Microscopy for Cell Membrane Investigation -- Physico-Chemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector -- In Vitro Testing of Anticancer Nanotherapeutics Using Tumor Spheroids
    Abstract: This volume details protocols on formulation, surface modification, characterization, and application of a variety of pharmaceutical nanocarriers such as micelles, nanoparticles, dendrimers, carbon dots, polymersomes, and others. Chapters are targeted toward investigators working in academic and industrial laboratories conducting research in the broad field of pharmaceutical sciences, with an emphasis on drug delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pharmaceutical Nanotechnology: Basic Protocols aims to be a source of inspiration to all investigators who are interested in the potential of the merger of nanotechnology with pharmaceutical sciences
    Pages: XII, 398 p. 131 illus., 98 illus. in color. : online resource.
    ISBN: 9781493995165
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  • 39
    facet.materialart.
    Cham : Springer
    Keywords: Human Genetics ; Biochemistry ; Biological models ; Human Genetics ; Protein Science ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Preface -- Chromatin regulation by long non-coding RNAs -- Regulation of Eukaryotic Cell Differentiation by Long Noncoding RNAs -- Roles of long non-coding RNAs in X-chromosome inactivation -- Roles of Long Non-Coding RNAs in Genomic Imprinting -- Dysregulation of long noncoding RNAs in human disease -- Functions of long non-coding RNAs in non-mammalian systems -- Emerging technologies to study long non-coding RNAs -- Long non coding RNAs and nuclear body formation and function -- The role of long non-coding RNAs in immunity -- Novel methods to study long non-coding RNAs -- OMICs of long non-coding RNAs -- Cancer epigenetics and long non-coding RNAs -- Roles of long non-coding RNAs in human development -- Index
    Abstract: This second edition shows how long non-coding RNAs (lnc)RNAs have emerged as a new paradigm in epigenetic regulation of the genome. Thousands of lncRNAs have been identified and observed in a wide range of organisms. Unlike mRNA, lncRNA have no protein-coding capacity. So, while their function is not entirely clear, they may serve as key organizers of protein complexes that allow for higher order regulatory events. Advances in the field also include better characterization of human long non-coding RNAs, novel insights into their roles in human development and disease, their diverse mechanisms of action and novel technologies to study them
    Pages: VII, 187 p. 10 illus., 9 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030170868
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  • 40
    Keywords: Entomology ; Veterinary Medicine ; Plant breeding ; Microbiology ; Bacteriology ; Mycology ; Veterinary Microbiology, Parasitology and Infectious Diseases ; Plant Breeding/Biotechnology ; Plant Systematics/Taxonomy/Biogeography ; Medical Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Preface -- 1. Fungal diseases of Animals -- 2. Factors influencing the development of infectious fungal disease in animals -- 3. Immunological diagnostic of fungal disease in animals -- 4. Molecular identification of fungal diseases of animals -- 5. PAN-PCR based diagnostic of fungal diseases of lab animals -- 6. Opportunistic invasive fungal infections of animals -- 7. Common practices to control fungal diseases of lab animals -- 8. Histopathologic Diagnosis of Fungal Infections of lab animals -- 9. Development of sandwich dot-ELISA/Serological tools for the detection of fungal diseases of animals.-10. Mycotoxins produced by different fungal species in animals -- 11. Detection of secondary metabolites (mycotoxins) during fungal infections to lab animals -- Index
    Abstract: Fungal growths affect both human and animal well-being. Many natural pathogens of laboratory animals alter host physiology, rendering the host unsuitable for experimental uses. While the number and prevalence of few pathogens have declined considerably, many still turn up in laboratory animals and represent unwanted variables in research. Investigators using laboratory animals in biomedical experimentation should be aware of the profound effects that many of these agents can have on research. What does the future hold regarding the natural pathogens of laboratory animals? The selection of an animal model must carefully address issues of the type of human disease to mimic, the parameters to follow, and the collection of the appropriate data to answer those questions being asked. Overall, animal models of fungal infection will continue to deepen our understanding of how these infections occur. This book provides a valuable source of information to biological and biomedical scientists and to clinical and doctoral researchers working in the area of fungal infections and diseases of laboratory animal species
    Pages: XII, 169 p. 26 illus., 23 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030185862
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  • 41
    Keywords: Medicine ; Biochemistry ; RNA-ligand interactions ; Pharmaceutical technology ; Molecular Medicine ; Medical Biochemistry ; Protein-Ligand Interactions ; Medicinal Chemistry ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Final ToC tbd. 1) Description of human carbonic anhydrases (12 catalytically active human isoforms; isoforms as drug targets, enzymatic activity, catalytic mechanism, thermodynamics of protonation of the water molecule in the active site) -- 2) Thermal stabilities of all isoform CA catalytic domains -- Thermodynamics of inhibitor binding to CAs (incl. intrinsic thermodynamics - not mentioned important subject in most CA literature) -- 3) Comparison of methods to determine inhibitor binding to CAs (ITC, FTSA, enzymatic activity assays) -- 4) Chemical synthesis of CA inhibitors -- 5) X-ray crystallographic structures of CAs and their complexes with inhibitors -- 6) Antibodies against CAs
    Abstract: This book offers deep insights into the thermodynamics and molecular structures of the twelve catalytically active isoforms of human carbonic anhydrase (CA) with a particular focus on inhibitor binding for drug design. X-ray crystallographic structures in combination with enzyme kinetic testing provide information on the interaction of CAs and their inhibitors, knowledge which is crucial for rational drug design. CAs are zinc carrying enzymes that catalyse the reversible interconversion of carbon dioxide and bicarbonate and are involved in numerous cellular processes. They are therefore a common target for drugs. The suppression of CA activities through inhibitory compounds has found application for example in diuretics and in glaucoma therapy. In this book methods used to determine binding thermodynamics of inhibitory compounds (Isothermal titration calorimetry, Fluorescent thermal shift assay/differential scanning fluorimetry and others) will be compared in detail. Also types and chemical synthesis of CA inhibitors, the use of antibodies against CAs as well as inhibitor application in animals are discussed
    Pages: XXVI, 353 p. 169 illus., 107 illus. in color. : online resource.
    ISBN: 9783030127800
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  • 42
    Keywords: Toxicology ; Psychopharmacology ; Neurosciences ; Pharmacology/Toxicology ; Psychopharmacology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Part 1: Overview and General Principles -- 1. Impact, Diagnosis, Phenomenology, and Biology -- Part 2: The Past – from antiquity to 1970 including TCAs and MAOIs -- 2. Tricyclic Antidepressants and Monoamine Oxidase Inhibitors: Are They Too Old for a New Look? -- Part 3: The Present- 1970 to 2016 -- 3. Clinical Implications of the STAR*D Trial -- 4. Pharmacogenomics and Biomarkers of Depression -- 5. Therapeutic Drug Monitoring of Antidepressants -- 6. Selective Serotonin Reuptake Inhibitors -- 7. Serotonin and Norepinephrine Reuptake Inhibitors -- 8. Neurostimulation Therapies -- 9. Current Role of Herbal and Natural Preparations -- Part 4: The future -- 10. Role of Inflammation in Depression and Treatment Implications -- 11. NMDA Antagonists for Treatment-Resistant Depression -- 12. Drug Development in Psychiatry: The Long and Winding Road from Chance Discovery to Rational Development -- 13. Other Antidepressants -- Part 5: Use of Antidepressants in Special Populations -- 14. Use of Antidepressants in Patients with Co-occurring Depression and Substance Use Disorders -- 15. Treatment of Depression in Women -- 16. Management of Late-Life Depression -- 17. The Use of Antidepressants in Bipolar Depression
    Abstract: This volume reviews the known neurobiology of depression and combines classic data on antidepressant treatments with modern theory on the physiology of depression. It also discusses novel mechanism of action drugs
    Pages: XII, 442 p. 17 illus., 8 illus. in color. : online resource.
    ISBN: 9783030109493
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  • 43
    Keywords: Oncology ; Medical virology ; Microbiology ; Zoology ; Cancer Research ; Virology ; Microbiology ; Zoology ; Springer eBooks
    Description / Table of Contents: Overview of virus infection and tumorigenesis -- Marine viruses -- Marine invertebrate stress responses to virus infection -- The roles of microRNAs in antiviral immunity of marine invertebrates -- Marine microbe stress responses to bacteriophage infection -- Roles of microbial metabolites in bacteriophage-microbe interactions -- Tumorigenesis and metabolism disorder -- Effects of microRNAs from marine invertebrate stress responses to virus infection on tumorigenesis -- Anti-tumor activities of secondary metabolites from marine microbe stress responses to virus infection
    Abstract: This book reviews the latest research on the molecules and mechanisms of marine host stress responses to viral infections and tumorigenesis. It offers an overview of the state of the art in the field as well as future directions. Metabolism disorder is a characteristic of tumorigenesis. Since viruses complete their life cycle in host cells, such infections cause metabolic disorders in the host. As such, the mechanisms of virus pathogenesis and tumor progression are similar or even identical. In essence, the role of antiviral molecules is to maintain the metabolic homeostasis of infected host cells, and the antiviral molecules induced by virus infection may play an important role in antitumor pathways, resulting in cancer cell death or restoring the disordered metabolism of cancer cells. The molecules generated during host stress responses to viruses can also contribute to the antitumor mechanisms in humans. However, the relationship between host stress responses to virus infection and tumorigenesis has not been extensively explored. In recent years, studies have shown that marine host stress responses to viral invasion can be good models for exploring human antitumor mechanisms. Stimulating further research in the field, this book offers graduate students and researchers with comprehensive insights into host stress responses to viral invasion and tumor progression. It is also a valuable resource for those working in the pharmaceutical industry interested in drug discovery based on molecules derived from host stress responses to viral infection
    Pages: V, 318 p. 8 illus., 7 illus. in color. : online resource.
    ISBN: 9789811361982
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  • 44
    Keywords: Cell Culture ; Regenerative Medicine/Tissue Engineering ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Part 1 : Isolation and culture of skin cells -- Chap 1: Isolation and Culture of Human Keratinocytes -- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture -- Chap 3 : Isolation and Culture of Epidermal Melanocytes -- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor -- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells -- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts -- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells -- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue -- Part 2 : Tissue engineering skin and engineered skin models -- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility -- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes -- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells -- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes -- Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering -- Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models -- Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin -- Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts -- Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy -- Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo -- Chap 18 : Human Reconstructed Skin in a Mouse Model -- Chap 19 : Pig Model to Test Tissue Engineered Skin -- Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model
    Abstract: This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine
    Pages: XII, 263 p. 79 illus., 66 illus. in color. : online resource.
    ISBN: 9781493994731
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  • 45
    Keywords: Metabolism ; Mass spectrometry ; Metabolomics ; Mass spectrometry ; Springer eBooks
    Description / Table of Contents: Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores by Mass Spectrometry -- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer -- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification -- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites -- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells -- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson’s Disease -- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease -- Proteometabolomics of Melphalan Resistance in Multiple Myeloma -- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading -- Protocols for NMR Analysis in Livestock Metabolomics -- Metabolomics Applicable to Retinal Vascular Diseases
    Abstract: The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field
    Pages: XV, 335 p. 82 illus., 53 illus. in color. : online resource.
    ISBN: 9781493994885
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    New York, NY : Springer
    Keywords: Pharmaceutical technology ; Biochemistry ; Cell Physiology ; Pharmaceutical Sciences/Technology ; Biochemistry, general ; Cell Physiology ; Springer eBooks
    Description / Table of Contents: Design Principles for Intestinal Permeability of Cyclic Peptides -- Strategies to Enhance Metabolic Stabilities -- Designing Cell-Permeable Macrocyclic Peptides -- Computational Methods for Studying Conformational Behaviors of Cyclic Peptides -- Computational Opportunities and Challenges in Finding Cyclic Peptide Modulators of Protein-Protein Interactions -- Design of Cyclic Peptides as Protein Recognition Motifs -- Design and Synthetic Strategies for Helical Peptides -- Click Chemistry for Cyclic Peptides Drug Design -- Frontier between Cyclic Peptides and Macrocycles -- Building upon Nature’s Framework: Overview of Key Strategies towards Increasing Drug-Like Properties of Natural Product Cyclopeptides and Macrocycles -- Design of Oxytocin Analogs -- DNA-Encoded Macrocyclic Peptide Library -- Peptide Display Technologies -- Discovery of Functional Macrocyclic Peptides by Means of the RaPID System -- Genetic Selections with SICLOPPS Libraries: Towards the Identification of Novel Protein-Protein Interaction Inhibitors and Chemical Tools
    Abstract: This book covers strategies to improve cell permeability, intestinal permeability, and metabolic stability, which are the typical liabilities associated with cyclic peptides, to enhance protein-protein recognition, and to build upon nature’s cyclic peptides and macrocycles. Chapters also cover key peptide screening and display strategies, as well as important synthetic approaches towards cyclic and helical peptides. Cyclic peptides have become of significant importance as chemical tools in biology and drug discovery, since this class of chemicals has become a credible alternative source of new drug leads on par with traditional small molecules. As a part of the Methods in Molecular Biology series, this collection includes the kind of detail and implementation advice to aid researchers in the field. Authoritative and cutting-edge, Cyclic Peptide Design serves as a critical resource and go-to reference for researchers within the pharmaceutical industry, as well as scientists and students in the bioorganic, medicinal, and natural product chemistry fields
    Pages: X, 332 p. 138 illus., 80 illus. in color. : online resource.
    ISBN: 9781493995042
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  • 47
    Keywords: Metabolism ; Human Genetics ; Lipids ; Bioinformatics ; Metabolomics ; Human Genetics ; Lipidology ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells -- High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods -- High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research -- Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications -- CE-MS for Metabolomics: A Comparison with Other Techniques -- Overview of Lipid Mass Spectrometry and Lipidomics -- LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins -- Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics -- HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle -- Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry -- Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples -- Untargeted Metabolomics of Plant Leaf Tissues -- Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling -- Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry -- Insights into Dynamic Network States Using Metabolomic Data -- Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics -- Using Stable Isotope Tracers for Metabolic Pathway Analysis -- Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics -- El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics -- Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data -- Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors -- Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity -- Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy -- Metabolomic Applications in Radiation Biodosimetry -- Metabolomics Analyses to Investigate the Role of Diet and Physical Training -- Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics -- Personalized Metabolomics
    Abstract: This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine
    Pages: XV, 459 p. 83 illus., 63 illus. in color. : online resource.
    ISBN: 9781493992362
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  • 48
    Keywords: Organizational Studies, Economic Sociology ; Sociological Theory ; Sociology of Culture ; Cultural Studies ; Springer eBooks
    Abstract: ́€œThis is the first serious book-length, comprehensive treatment of the role of society and the cultural imperatives that undergird the Greek sovereign debt drama and the countrý€™s inability to climb out of it. Deftly mixing relevant sociological literature with key concepts from anthropology, psychology, religion, and political science, this volume is a sophisticated, strongly substantiated, theoretically and empirically grounded work that will fill a void in the literature on Greece and beyond.́€ ́€”Constsantine P. Danopoulos, Professor Emeritus of Political Science and President́€™s Scholar, San Jose State University, USA This original analysis of modern Greec這s political culture attempts to present a ́€œtotal social fact́€́€”a coherent and complex representation of Greek socio-political cultur途to identify the cultural causes of Greec這s recent disastrous economic crisis. Using a culturalist frame inspired by the Yale Strong Program, Marangudakis argues that the core cultural orientations of Greece have determined its politicś€”Greek secular culture flows out of the religion of Eastern Orthodoxy with its mysticism, icons, and general €œórtherworldly-nesses.́€ This theoretical discussion, bringing together Eisenstadt, Michael Mann, Banfield, and Taylor, is complemented by an innovative use of survey data, processed by political scientist and statistician . The carefully deployed quantitative data demonstrate that the culture previously described is actually shared by people living in Greece today. In his sweeping conclusion to this thorough cultural analysis, Marangudakis reflects on the prospects of Greek cultural recovery through the construction of a non-populist civil religion
    Pages: XXX, 460 p. 99 illus., 45 illus. in color. : online resource.
    ISBN: 9783030135898
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  • 49
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Microbiology ; Biotechnology ; Biochemistry ; Microbial Genetics and Genomics ; Microbial ecology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Springer Nature Living Reference
    Abstract: This book is a compilation of the most relevant molecular mechanisms and cellular processes that are involved in the infection processes in relation to lipid metabolism. The authors are international experts in the field of infection biology. Readers will understand infection metabolism and the contribution of lipids and lipid-protein interaction to lipids. Microbial lipids play an important role in almost all cellular phenomena. Microbial infections also contain an important virulence component in microbial lipids. The secretion of lipid vesicles that contain virulence factors, the assembly of lipid membrane microdomains harboring signal transduction pathways relate to infection process and the number of lipid-protein interactions that are necessary for the internalization of pathogens to host cells are some examples of the importance of lipids and lipid metabolism in the development of infections
    Pages: : online resource.
    ISBN: 9783319724737
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  • 50
    Keywords: Medicine ; Biomedicine general ; Springer Nature Living Reference
    Description / Table of Contents: Methods and Models for Studying Immunosenescence -- Methods for studying human immunosenescence in tissues other than blood -- Mechanisms - Receptors and Signal Transduction -- Clinical Relevance in Disease States – Infection -- Modulation -- Nutrition
    Abstract: €œÍmmunosenescenc逝 is an imprecise term used to describe deleterious age-associated changes to immune parameters observed in all mammals studied so far. It represents a rapidly progressing science in the aging field, with a vertiginous volume of new data, knowledge and concepts concerning these changes. Indeed, during the almost 10 years that have passed since the first edition of this Handbook, a wealth of additional information has emerged from animal models and also from increasingly available human data. Numerous new aspects of the immune changes with aging have emerged, while others became less prominent. The application of the new multi-€œómicś€ techniques in immunology have revolutionized our molecular understanding of basic immune mechanisms. The extended and revisited concept of Inflamm-aging sheds a new light on the intricate relationships among immunosenescence and inflamm-aging. The new concept of inflamm-aging integrates different emerging experimental data such as those pertaining to the senescence-associated secretory phenotype and responses to damage. ́€œGeroscienc逝 is emphasising the role of immune changes during life in all manner of age-related chronic diseases such as cancer and neurodegenerative disorders. The field is poised to be in a position to translate these accumulated data into the clinical setting via a better understanding of the contribution of immunosenescence to age-associated pathologies, and their prevention by appropriate interventions. This new edition of the Handbook seeks to encompass the current state of our knowledge on the multitude of those changes to immunity related to aging, with contributions from experts in both basic research and clinical areas. This book therefore considers methods and models for studying immunosenescence; cellular immunosenescence of T cells, B cells, neutrophils, antigen presenting cells, NK, NKT and stem cells; genetics; mechanisms including receptors and signal transduction; mitochondria; proteasome; cytokines; neuro-endocrine-immune networks; inflammation; thymus; clinical relevance in disease states including infections, autoimmunity, cancer, metabolic syndrome, neurodegenerative diseases, frailty and osteoporosis; modulation by nutrition, microbiome, lipids, vaccination and the burning question ́€œcan interventions to influence immunosenescence be realistically proposed based on our current state of knowledge?́€&#x9d
    Pages: : online resource.
    ISBN: 9783319645971
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  • 51
    Keywords: Microbiology ; Biotechnology ; Biodiversity ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biodiversity ; Springer Nature Living Reference
    Description / Table of Contents: A Genomic View of the Catabolism of Aromatic Compounds in Pseudomonas -- A Genomic View of the Catabolism of Short Chain Alkanes -- Aerobic Degradation of Aromatic Hydrocarbons -- Aerobic Degradation of Aromatic Hydrocarbons: Enzyme Structures and Catalytic Mechanisms -- Aerobic Degradation of Chloroaromatics -- Aerobic Degradation of Gasoline Ether Oxygenates -- Aerobic Degradation of Halogenated Aliphatics -- Biochemistry and Molecular Biology of Methane Monooxygenase -- Biosynthesis, Insertion and Function of Fe-S Cofactors -- Biosynthesis, Insertion and Function of Flavin Cofactors -- Biosynthesis, Insertion and Function of haem-iron Prosthetic Groups -- Computational Framework for Integration of Lipidomics Data into Metabolic Pathways -- Degradation of Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) -- Degradation of Rubber/Polyisoprene -- Diversity and Common Principles in Enzymatic Activation of Hydrocarbons: An Introduction -- Energetic and Other Quantitative Aspects of Microbial Hydrocarbon Utilization: An Introduction: -- Enzymes for Aerobic Degradation of Alkanes in Bacteria -- Enzymes for Aerobic Degradation of Alkanes in Yeasts -- Evolution of New Catabolic Functions Through Gene Assembly by Mobile Genetic Elements -- Experimental Evolution of Novel Regulatory Activities in Response to Hydrocarbons and Related Chemicals -- Functional Gene Diversity, Biogeography, Dynamics -- Genetic Features and Regulation of n-Alkane Metabolism in Bacteria -- Genetic Features and Regulation of n-Alkane Metabolism in Yeasts -- Genetic Features of Methylotrophs -- Genetics and Functional Genomics of Aerobic Degradation of Hydrocarbons: An Introduction -- Genetics and Molecular Features of Bacterial Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) Transformations -- Genetics of Biphenyl Biodegradation and Co-Metabolism of PCBs -- Genetics of Rubber/Polyisoprene Degradation -- Genetics and Ecology of Isoprene Degradation -- Genomic View of Mycobacterial High Molecular Weight Polycyclic Aromatic Hydrocarbon Degradation -- Global Aerobic Degradation of Hydrocarbons in Aquatic Systems -- Global Aerobic Degradation of Hydrocarbons in Terrestrial Systems -- Global Consequences of the Microbial Production and Consumption of Inorganic and Organic Sulfur Compounds -- Key Features of Aerobic Hydrocarbon Biodegrader Genomes -- Lipid Degradation - Lipid Cycles -- Lipolytic Enzymes from Bacteria -- Lipolytic Enzymes from Yeasts -- Metabolism of Steroids -- Oxidative Inactivation of Ring-Cleavage Extradiol Dioxygenases: Mechanism and Ferredoxin-Mediated Reactivation -- Pathways for the Degradation of Fatty Acids in Bacteria -- Phylogenomics of Aerobic Bacterial Degradation of Aromatics -- Physiology and Biochemistry of the Aerobic Methane Oxidizing Bacteria -- Potential for Microbial Interventions to Reduce Global Warming -- Rational Construction of Bacterial Strains with New/Improved Catabolic Capabilities for the Efficient Breakdown of Environmental Pollutants -- Regulation of the Degradation of Fatty Acids in Bacteria -- Regulation of the Degradation of Fatty Acids in Yeast -- Stereochemistry of Hydrocarbon-transforming Enzyme Reactions -- Structure-Function Relationships and Engineering of Haloalkane Dehalogenases -- Transcriptional Control of the TOL Plasmid Pathways.-
    Abstract: In this book international experts discuss the state-of-the-art in the biological degradation of hydrocarbons to meet remedial or disposal goals. The work focuses on practical applications, often on globally important scales including the remediation of some of the world’s largest crude oil spills. Other related chapters discuss important implications of microbial transformation of hydrocarbons, including treatment of high fat processing wastes, impacts of microbial biodegradation activity on industrial processes, and the implications of microbial oil degradation in relation to modern oil extraction processes like hydraulic fracturing of shales and extraction of oil sands
    Pages: 100 illus., 50 illus. in color. : online resource.
    ISBN: 9783319445359
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  • 52
    Keywords: Medicine ; Toxicology ; Pharmaceutical technology ; Life sciences ; Biochemistry ; Animal Physiology ; Biomedicine general ; Pharmacology/Toxicology ; Pharmaceutical Sciences/Technology ; Life Sciences, general ; Animal Biochemistry ; Animal Physiology ; Springer Nature Living Reference
    Abstract: This volume provides an overview of microbial toxins from diverse bacterial and fungal origins. These molecules, produced by various species and consisting of protein or small organic molecules, can play a pivotal role in pathogenesis of plants, animals, and humans that in turn can lead to the survival/dissemination of the host microbe. Many of these microbes, due to their toxins, impact our society from a health and economic perspective. In particular, this volume address the diverse niches of these organisms focused upon their associated toxins. The structures, functions, and genetics of toxins will be addressed. Besides the ill-effects elicited by these toxins, it must be noted that there is immense potential for turning some of these harmful molecules into useful tools as specific probes (of receptors), novel drugs or drug-conjugates for the treatment of various diseases, and immunomodulating agents for targeted vaccine delivery for research and unique medicines. Recent progress in bacterial genome mapping and establishment of three-dimensional crystal structures of several bacterial toxins provides a deeper knowledge and greater understanding of structure-function relationships. Moreover, the emergence of some bacteria (i.e., Bacillus anthracis, Burkholderia pseudomallei, Staphylococcus aureus), and their toxins, as biological weapons also necessitates a thorough understanding of these agents, their pathophysiology, and development of countermeasures. This volume will also be a common resource for researchers interested in many other medically-relevant microorganisms, and their toxins, that include Clostridium botulinum, C. difficile, C. perfringens, C. tetani, Escherichia coli, Helicobacter pylori, and Listeria monocytogenes.℗ Recent studies have correlated the effect of global warming and climate change as a trigger for natural disasters and impact on human health via emergence of various vector-borne and infectious diseases caused by toxin-producing microbes. The 2003 Institute of Medicine (IOM) report, ́€œMicrobial Threats to Health,́€ identified the influence of changing ecosystem on the emergence and spread of infectious diseases and economic development of specific regions of the world.℗ This current handbook is a major reference work on microbial toxins written by a panel of International experts and organized into 24 chapters
    Pages: Approx. 550 p. 30 illus., 15 illus. in color. : online resource.
    ISBN: 9789400767256
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