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  • Springer  (1,652,126)
  • American Institute of Physics (AIP)  (154,286)
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  • 1
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 2
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single Molecule FRET Methods to Study Glutamate Receptors -- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) -- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy -- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors -- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function -- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) -- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus -- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats -- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction -- Gene Expression Analysis by Multiplex Single Cell RT-PCR -- Metabolomics Analysis of Glutamate Receptor Function -- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation -- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function -- Fractionation of Subcellular Compartments from Human Brain Tissue -- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
    Abstract: This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications
    Pages: X, 260 p. 67 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990771
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  • 3
    Call number: WN180:8
    Keywords: Diagnostic Imaging ; Artificial intelligence
    Pages: xv, 373 p. : ill.
    ISBN: 9783319948775
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    WN180:8 on loan
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  • 4
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    Heidelberg : Springer
    Call number: QH442:222 ; ERW
    Keywords: Genetic Techniques
    ISBN: 9783642346934
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    QH442:222 available
    ERW in acquisition
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  • 5
    Keywords: Agriculture ; Plant breeding ; Plant genetics ; Nutrition ; Agriculture ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Nutrition ; Springer eBooks
    Description / Table of Contents: Preface -- Foreword -- 1. Biofortification of Staple Food crops: An overview -- 2. Marker assisted Breeding for improving the cooking and eating quality of rice -- 3. Improving the nutritional value of potatoes by conventional breeding and genetic modification -- 4. Conventional and molecular breeding approaches for biofortification of pearl millet -- 5. Genetic approaches to improve common bean nutritional quality: current knowledge and future perspectives -- 6. Marker assisted breeding for Beta carotene rich Maize Hybrids -- 7. Recent Advances in Breeding for Modified Fatty Acid Profile in Soybean Oil -- 8. Breeding dry beans with improved cooking and canning quality traits -- 9. Genomic approaches for Biofortification of wheat grain with iron and zinc -- 10. Current Breeding Approaches for Developing Rice with Improved Grain and Nutritional Qualities -- 11. Quality Protein Maize for Nutritional Security -- 12. Genetic improvement for end use quality in wheat -- 13. The use of modern molecular biology and biotechnology tools for improving the Quality value of oilseed Brassicas -- Index
    Abstract: Development of superior crops that have consistent performance in quality and in quantity has not received the same emphasis in the field of genetics and breeding as merited. Specialty trait requires special focus to propagate. Yet basic germplasm and breeding methodologies optimized to improve crops are often applied in the development of improved specialty types. However, because of the standards required for specialty traits, methods of development and improvement are usually more complex than those for common commodity crops. The same standards of performance are desired, but the genetics of the specialty traits often impose breeding criteria distinct from those of non-specialty possessing crops. Specifically, quality improvement programs have unique characteristics that require careful handling and monitoring during their development for specific needs. Adding value either via alternative products from the large volumes of grain produced or development of specialty types is of interest to producers and processors. This work assimilates the most topical results about quality improvement with contemporary plant breeding approaches.The objective of this book is to provide a summary of the germplasm, methods of development, and specific problems involved for quality breeding. In total, fourteen chapters, written by leading scientists involved in crop improvement research, provide comprehensive coverage of the major factors impacting specialty crop improvement
    Pages: XVII, 275 p. 26 illus., 17 illus. in color. : online resource.
    ISBN: 9783030046095
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  • 6
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    Singapore : Springer
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Protective Responses of Different Organs to Environmental Toxicants or Stresses -- Avoidance Behaviour of Nematodes to Environmental Toxicants or Stresses -- Intestinal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Epidermal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Toxicity Induction in Intestine and Epidermis in Nematodes Exposed to Environmental Toxicants or Stresses -- Toxicity Induction in Neurons and Muscle in Nematodes Exposed to Environmental Toxicants or Stresses -- Reproductive Toxicity Induction in Nematodes Exposed to Environmental Toxicants or Stresses -- Intestinal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Epidermal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Neuronal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Germline Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses
    Abstract: This book introduces readers to intestinal and epidermal barriers, and to toxicity induction of environmental toxicants or stresses in the intestine, epidermis, neurons, muscle, and reproductive organs in Caenorhabditis elegans. In addition, it discusses the protective responses of various organs and nematodes’ avoidance behaviour with regard to environmental toxicants or stresses. The intestinal, epidermal, neuronal, and germline signalling pathways required for the regulation of toxicity of environmental toxicants or stresses are also introduced and discussed. As a classic model animal with well-described genetic and developmental backgrounds, the nematode Caenorhabditis elegans has been successfully and widely used in both toxicity assessments and toxicological studies on various environmental toxicants and stresses. Once exposure to certain environmental toxicants has occurred, the toxicants can enter into the primary targeted organs (such as intestinal cells), and even be translocated into secondary targeted organs (such as reproductive organs and neurons). Based on related available data, this book provides a systematic understanding of target organ toxicology in C. elegans
    Pages: XIII, 337 p. 232 illus., 175 illus. in color. : online resource.
    ISBN: 9789811360107
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  • 7
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 8
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 9
    Keywords: Biological models ; Proteomics ; Gene Expression ; Systems Biology ; Proteomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: 1. The Potential of €˜Ómics to Link Lipid Metabolism and Genetic and Comorbidity Risk Factors of Alzheimeŕ€™s Disease in African Americans -- 2. The Role of Biomarkers in Alzheimeŕ€™s Disease Drug Development -- 3. Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- 4. Lymphocytes, Platelets, Erythrocytes and Exosomes as Possible Biomarkers for Alzheimer's Disease Clinical Diagnosis -- 5. Genetic Risk Factors for Alzheimer Disease: Emerging Roles of Microglia in Disease Pathomechanisms -- 6. Neuroimaging Studies of Cognitive Function in Schizophrenia -- 7. The Role of Biomarkers in Psychiatry -- 8. Interactome Studies of Psychiatric Disorders -- 9. MicroRNAs in Major Depressive Disorder -- 10. Proteomic Markers for Depression -- 11. Advances in Biomarker Studies in Autism Spectrum Disorders -- 12. Proteomic Investigations of Autism Spectrum Disorder: Past Findings, Current Challenges and Future Prospects -- 13. Role of the Gut Microbiome in Autism Spectrum Disorders -- 14. Metabolomic Biomarkers in Mental Disorders: Bipolar Disorder and Schizophrenia -- 15. Early Detection and Treatment of Patients with Alzheimeŕ€™s Disease: Future Perspectives
    Abstract: This book includes a series of reviews on general aspects of biomarker use in the study of psychiatric and neurodegenerative diseases and the development of medications involved in their treatment. It describes the pros and cons of the various approaches and covers the successes and failures in this research field. It is only by a thorough understanding of the shortcomings that progress can be made. The overall goal is to facilitate the understanding and treatment of these disorders, by providing a viable mechanism of catching up with other areas of modern medicine, such as diabetes and heart disease. Finally, it is anticipated that the development and application of valid biomarker tests and the leveraging of novel drug targets will help the fields of psychiatry on neurodegenerative disorders move into the area of personalized medicine where the right patients can receive the right medication at the right time for the best possible outcome
    Pages: XIII, 328 p. 28 illus., 26 illus. in color. : online resource.
    ISBN: 9783030055424
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  • 10
    Keywords: Plant breeding ; Plant genetics ; Agriculture ; Genetic Engineering ; Alternative Medicine ; Pharmaceutical technology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Agriculture ; Genetic Engineering ; Complementary & Alternative Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Foreword by Prof. M. Iqbal Choudhary, Director, International Center for Chemical and Biological Sciences -- Marine and Freshwater Organisms in Drug Discovery -- Bioactive Molecules from Malaysian Flora -- Immunomosulatory Effect of Cycloartane-Type Triterpenes -- Antigiardial Actitivty of Citrullus lanatus Extracts -- Potential Idiosyncratic Liver Toxicity of Herbal Products -- Antitrypanosomal Activity of Nigerian Plants -- Alkaloids Isolated from Malaysian Plant Families -- Pharmacological Studies on Ficus Species -- Herbals in the treatment of Salmonella typhi -- Biology of Proline-Containing Cyclic Peptides -- Plant Extracts and Memory Impairment -- Herbals Used in Heat and Detoxification in Chinese Traditional Medicine -- Secondary Metabolites from Solanaceae of Iran -- Molecular Docking Study on Rauvolfia species -- Drug Development from the farm -- Phytochemical Screening of Sri Lankan Plants -- New carbohydrate esters of cinnamic acid from the leaves of Gleditschia -- Herbals in the Treatment of Alzheimer's disease
    Abstract: Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the “scientific methods”. Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, it will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture
    Pages: XX, 385 p. 67 illus., 45 illus. in color. : online resource.
    ISBN: 9783030044084
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  • 11
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    Cham : Springer
    Call number: QZ200:576 ; C100:50
    Pages: vii, 205 p. : ill.
    ISBN: 9783030012069
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    QZ200:576 available
    C100:50 departmental collection or stack – please contact the library
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  • 12
    Keywords: Emerging infectious diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Analysis of Iron Requirements and Siderophore Production -- The Pathometabolism of Legionella Studied by Isotopologue Profiling -- Quantification of Viable but Non-culturable Cells of Legionella pneumophila -- Selection of Legionella Virulence-related Traits by Environmental Protozoa -- Migration of Acanthamoeba castellanii through Legionella Biofilms -- Scar-free Genome Editing in Legionella pneumophila -- Querying Legionella Genomes using Transposition-sequencing -- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq -- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae -- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium -- Sorting of Phagocytic Cells Infected with Legionella pneumophila -- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment -- Mitochondrial Dynamics and Activity in Legionella-Infected Cells -- Perturbation of Legionella Cell Infection by RNA Interference -- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila -- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography -- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila -- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification -- Inflammasome Activation in Legionella-infected Macrophages -- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila -- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence -- Dictyostelium Host Response to Legionella Infection: Strategies and Assays -- 6.1.3 The Caenorhabditis elegans model of Legionella infection -- The Mouse as a Model for Pulmonary Legionella Infection -- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples -- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients -- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry
    Abstract: The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens
    Pages: XXVI, 476 p. 74 illus., 50 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990481
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  • 13
    Keywords: Biochemistry ; Medical laboratories ; Protein Science ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Using Data Mining to Explore Calmodulin Bibliography -- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator -- High Resolution Calcium Imaging Method for Local Calcium Signaling -- Measurement of Contractility and Calcium Release in Cardiac Spheroids -- Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis -- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio) -- Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements -- Designing Calcium Binding Proteins for Molecular MR Imaging -- Coordination to Divalent Cations by Calcium Binding Proteins -- Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo -- Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets -- Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes -- Expression and Purification of Calmodulin for NMR and Other Biophysical Applications -- The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells -- Calpain Purification through Calpastatin and Calcium: Strategy and Procedures -- Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins -- Expression and Characterization of MICU2, a Ca2+ Sensor Protein -- S100 Proteins in the Innate Immune Response to Pathogens -- Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors -- Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy -- Isolation and Characterization of S100 Protein-Protein Complexes -- The Multifaceted S100A4 Protein in Cancer and Inflammation -- Interaction of S100A6 with Target Proteins In Vitro and in Living Cells -- Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7) -- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin -- Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography -- Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex -- Analysis of S100A11 in DNA Damage Repair -- Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography -- Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure -- High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins -- Calcium-Induced Protein Folding in Calumenin and Calmodulin -- Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing -- Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies -- Tryptophan Scanning Mutagenesis of EF-Hand Motifs -- Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling -- Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level -- Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation -- Characterization of Calcium-Binding Proteins from Parasitic Worms -- High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome -- Targeted Mass Spectrometry of S100 Proteins -- Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury -- Serum S100B Levels in Melanoma -- The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine -- S100A7 in Psoriasis: Immunodetection and Activation by CRISPR -- S100A8/A9 in Myocardial Infarction -- Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults -- S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions
    Abstract: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry
    Pages: XIX, 779 p. 175 illus., 117 illus. in color. : online resource.
    ISBN: 9781493990306
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  • 14
    Keywords: Oncology ; Immunology ; Cancer Research ; Immunology ; Springer eBooks
    Description / Table of Contents: Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry
    Abstract: This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies
    Pages: XI, 225 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493989799
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  • 15
    Keywords: Plant breeding ; Agriculture ; Plant Breeding/Biotechnology ; Agriculture ; Springer eBooks
    Description / Table of Contents: Hybrid Sorghum Product Development and Production -- Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum -- Field Studies to Develop Weed Management Programs for Grain Sorghum -- Discovery of Sorghum Haploid Induction System -- Development of a Pedigreed Sorghum Mutant Library -- Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion -- Assaying Sorghum Nutritional Quality -- Tannin Analysis in Sorghum Grains -- Sorghum Phytochemicals and their Potential Impact on Human Health -- Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography -- Gene Editing in Sorghum through Agrobacterium -- Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment -- Novel Ternary Vectors for Efficient Sorghum Transformation -- Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa -- Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions -- Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case -- Background on Grain Sorghum Usage -- Assaying Sorghum for Fuel Production -- The Role of Sorghum in Renewables and Biofuels
    Abstract: This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum
    Pages: X, 281 p. 84 illus., 61 illus. in color. : online resource.
    ISBN: 9781493990399
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  • 16
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography -- Bacterial Expression and Purification of Calpains -- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity -- FRET-Based Assays to Determine Calpain Activity -- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer -- Determining Temporal and Spatial Expression of Calpains in Amphibians -- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis -- Expression and Activity of Calpain A in Drosophila melanogaster -- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1) -- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence -- CalCleaveMKL: A Tool for Calpain Cleavage Prediction -- Identification of Calpain-Activated protein Functions -- Production and Purification of Recombinant Calpastatin -- Isolation of Endogenous Calpastatin -- Immunoblotting for Calpastatin Expression -- Experimental Manipulation of Calpain Activity In Vitro -- In Vivo Calpain Knockdown using Delivery of siRNA -- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus -- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians -- Genetic Models of Calpain Deficiency and Ectopic Expression
    Abstract: This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases
    Pages: XII, 276 p. 66 illus., 37 illus. in color. : online resource.
    ISBN: 9781493989881
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  • 17
    Keywords: Bioinformatics ; Human Genetics ; Bioinformatics ; Human Genetics ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: ncRNAs in Inflammatory and Infectious Diseases -- p73-Governed miRNA Networks: Translating Bioinformatics Approaches to Therapeutic Solutions for Cancer Metastasis -- Methods for Annotation and Validation of Circular RNAs from RNAseq Data -- Methods to Study Long Non-coding RNA Expression and Dynamics in Zebrafish using RNA Sequencing -- Workflow Development for the Functional Characterization of ncRNAs -- ncRNA Editing: Functional Characterization and Computational Resources -- Computational Prediction of Functional microRNA – mRNA Interactions -- Tools for Understanding miRNA-mRNA Interactions for Reproducible RNA Analysis -- Computational Resources for Prediction and Analysis of Functional miRNA and their Targetome -- Non-coding RNAs Databases: Current Status and Trends -- Controllability Methods for Identifying Associations between Critical Control ncRNAs and Human Diseases -- Network-based Methods and Other Approaches for Predicting lncRNA Functions and Disease Associations -- Integration of miRNA and mRNA Expression Data for Understanding Etiology of Gynecologic Cancers -- Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks -- Kinetic Modeling of Competition and Depletion of Shared miRNAs by Competing Endogenous RNAs -- Modeling ncRNA-mediated Circuits in Cell Fate Decision -- Modeling Long ncRNA-mediated Regulation in the Mammalian Cell Cycle
    Abstract: This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field
    Pages: XII, 451 p. 78 illus., 65 illus. in color. : online resource.
    ISBN: 9781493989829
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  • 18
    Keywords: Human Physiology ; Human Physiology ; Springer eBooks
    Abstract: In recent years, volumes of the long-established Springer Subcellular Biochemistry series have shown great diversity of content, thus interesting biochemical topics, enthusiastically edited in all cases, have been readily accommodated. This new volume of the Subcellular Biochemistry series, Biochemistry and Cell Biology of Ageing: Part II, offers a broad overview of many different Clinical Science aspects of Ageing. The 17 chapters included in the book, contributed by knowledgeable authors, review many important topics at an advanced level. Whilst it has proved impossible to include all relevant topics, there is much present that will be of value to the reader. The book contains chapters on: “Poor Early Growth and Age-associated Disease; The Immune System and its Dysregulation with Ageing; Pulmonary Diseases and Ageing; Neurodegenerative Diseases and Ageing; Ageing and Cognition; Ageing and Osteoarthritis; Down Syndrome, Ageing and Epigenetics; The Vestibular System and Ageing; Signal Transduction, Ageing and Disease; Skin Changes During Ageing; Connective Tissue and Age-related Diseases ; Potential Cellular and Biochemical Mechanisms of Exercise and Physical Activity on the Ageing Process; Health Benefits of Anti-ageing Drugs; Visual Defects and Ageing; Hutchinson-Gilford Progeria Syndrome: Challenges at Bench and Bedside; Osteoporosis and the Ageing Skeleton; Neurovascular Ageing and Age-related Diseases” The book is primarily directed to advanced biomedical science and medical students, postgraduates, researchers, clinicians and academics in the field of Ageing. An earlier companion volume of the Subcellular Biochemistry series - Biochemistry and Cell Biology of Ageing: Part I (Volume 90) - covering the Biomedical Science aspects of ageing, has also been recently published
    Pages: X, 499 p. 53 illus., 47 illus. in color. : online resource.
    ISBN: 9789811336812
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  • 19
    Keywords: Otorhinolaryngology ; Neurosciences ; Otorhinolaryngology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Visual Influence on Auditory Perception -- Cue Combination Within a Bayesian Framework -- Toward a Model of Auditory-Visual Speech Intelligibility -- An Object-Based Interpretation of Audiovisual Processing -- Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway -- Multisensory Processing in the Auditory Cortex -- Audiovisual Integration in the Primate Prefrontal Cortex -- Using Multisensory Integration to Understand the Human Auditory Cortex -- Combining Voice and Face Content in the Primate Temporal Lobe -- Neural Network Dynamics and Audiovisual Integration -- Cross-Modal Learning in the Auditory System -- Multisensory Processing Differences in Individuals with Autism Spectrum Disorder
    Abstract: Auditory behavior, perception, and cognition are all shaped by information from other sensory systems. This volume examines this multi-sensory view of auditory function at levels of analysis ranging from the single neuron to neuroimaging in human clinical populations. Visual Influence on Auditory Perception Adrian K.C. Lee and Mark T. Wallace Cue Combination within a Bayesian Framework David Alais and David Burr Toward a Model of Auditory-Visual Speech Intelligibility Ken W. Grant and Joshua G. W. Bernstein An Object-based Interpretation of Audiovisual Processing Adrian K.C. Lee, Ross K. Maddox, and Jennifer K. Bizley Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway Shawn M. Willett, Jennifer M. Groh, Ross K. Maddox Multisensory Processing in the Auditory Cortex Andrew J. King, Amy Hammond-Kenny, Fernando R. Nodal Audiovisual Integration in the Primate Prefrontal Cortex Bethany Plakke and Lizabeth M. Romanski Using Multisensory Integration to Understand Human Auditory Cortex Michael S. Beauchamp Combining Voice and Face Content in the Primate Temporal Lobe Catherine Perrodin and Christopher I. Petkov Neural Network Dynamics and Audiovisual Integration Julian Keil and Daniel Senkowski Cross-Modal Learning in the Auditory System Patrick Bruns and Brigitte Röder Multisensory Processing Differences in Individuals with Autism Spectrum Disorder Sarah H. Baum Miller, Mark T. Wallace Adrian K.C. Lee is Associate Professor in the Department of Speech & Hearing Sciences and the Institute for Learning and Brain Sciences at the University of Washington, Seattle Mark T. Wallace is the Louise B McGavock Endowed Chair and Professor in the Departments of Hearing and Speech Sciences, Psychiatry, Psychology and Director of the Vanderbilt Brain Institute at Vanderbilt University, Nashville Allison B. Coffin is Associate Professor in the Department of Integrative Physiology and Neuroscience at Washington State University, Vancouver, WA Arthur N. Popper is Professor Emeritus and research professor in the Department of Biology at the University of Maryland, College Park Richard R. Fay is Distinguished Research Professor of Psychology at Loyola University, Chicago
    Pages: XVI, 272 p. 70 illus., 49 illus. in color. : online resource.
    ISBN: 9783030104610
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  • 20
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Origin and Pathogenesis of B Cell Lymphomas -- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma -- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells -- PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations -- Expression Cloning of Antibodies from Single Human B Cells -- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR -- Stereotyped B Cell Immunoglobulins in B Cell Lymphomas -- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies -- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR -- Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input -- FISH and FICTION in Lymphoma Research -- NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma -- RNA Sequencing in B-Cell Lymphomas -- Studying Cancer Heterogeneity by Single Cell RNA-Sequencing -- Evaluation of Whole Genome Sequencing Data -- Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines -- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens -- Proximity Ligation Assay -- Phospoproteomic Analysis of Signaling Pathways in Lymphomas -- Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries
    Abstract: This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology
    Pages: XII, 439 p. 66 illus., 48 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991518
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  • 21
    Keywords: Biomedical Engineering ; Microbiology ; Biomedical Engineering/Biotechnology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Pathway and Gene Discovery from Natural Hosts and Organisms -- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity -- Antisense RNA Elements for Downregulating Expression -- Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering -- Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs -- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae -- Assembly of Complex Pathways using Type IIs Restriction Enzymes -- Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly -- Checks and Balances with Use of the Keio Collection for Phenotype Testing -- Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803 -- Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica -- Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization -- Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization -- A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models -- 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms -- A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast
    Abstract: This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications
    Pages: X, 252 p. 46 illus., 39 illus. in color. : online resource.
    ISBN: 9781493991426
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  • 22
    Keywords: Biotechnology ; Biochemistry ; Biotechnology ; Protein Science ; Springer eBooks
    Description / Table of Contents: Established and Upcoming Yeast Expression Systems -- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts -- Pichia pastoris Promoters -- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes -- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha -- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica -- High-throughput Screening and Selection of Pichia pastoris Strains -- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke -- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori -- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae -- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast -- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production -- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments -- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris -- Efficient Development of a Mixed Feed Process for Pichia pastoris -- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification -- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants -- LC-MS analysis of (glyco)-proteins of Pichia pastoris -- Assessing the Quality of Recombinant Products Made in Yeast -- Quantification of Recombinant Products In Yeast
    Abstract: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications
    Pages: XII, 431 p. 60 illus., 46 illus. in color. : online resource.
    ISBN: 9781493990245
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  • 23
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    New York : Springer
    Keywords: Medicine ; Orthopedics ; Molecular Medicine ; Orthopedics ; Springer eBooks
    Description / Table of Contents: Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone
    Abstract: This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research
    Pages: XV, 670 p. 172 illus., 121 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493989973
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  • 24
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 25
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 26
    Keywords: Bacteriology ; Enzymes ; Immunology ; Bacteriology ; Enzymology ; Immunology ; Springer eBooks
    Description / Table of Contents: 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus
    Abstract: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets
    Pages: XIII, 312 p. 70 illus., 41 illus. in color. : online resource.
    ISBN: 9781493991549
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  • 27
    Keywords: Biochemistry ; Cytology ; Protein Science ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cloning and Mutagenesis Strategies for Large Collagens -- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen -- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds -- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils -- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy -- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry -- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes -- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics -- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy -- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology -- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics -- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix -- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans -- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis -- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment -- FACS Analysis of Col1α Protein Levels in Primary Fibroblasts -- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors
    Abstract: This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study
    Pages: XI, 254 p. 60 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990955
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  • 28
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: An Overview of CE in Clinical Analysis -- Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies -- Monitorization of α1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF Detection -- Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic Injection -- On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin -- Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis -- The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection -- Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization -- A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of Experiment -- New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS) -- Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Sample -- Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis -- Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis -- Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions -- A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood -- Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA) -- Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis -- Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen -- Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins
    Abstract: This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research
    Pages: XII, 273 p. 74 illus., 51 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992133
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  • 29
    Keywords: Medicine ; Toxicology ; Cell Physiology ; Endocrinology ; Metabolic Diseases ; Molecular Medicine ; Pharmacology/Toxicology ; Cell Physiology ; Endocrinology ; Metabolic Diseases ; Springer eBooks
    Description / Table of Contents: Part 1. Origin of Brown and Beige Adipocytes -- 1. Brown Adipose Tissue Development and Metabolism -- 2. Lessons from Cre-Mice and Indicator Mice -- 3. Aging of Brown and Beige/Brite Adipose Tissue -- 4. Adipogenesis in Primary Cell Culture -- 5. In Vitro Models for Study of Brown Adipocyte Biology -- 6. Brown-Like Adipocyte Progenitors Derived from Human iPS Cells: A New Tool for Anti-obesity Drug Discovery and Cell-Based Therapy? -- 7. Brown Adipose Tissue in Human Infants -- Part 2. Molecular Mechanisms of BAT Function and Signaling -- 8. Evolution of UCP1 -- 9. The Mechanism FA-Dependent H+ Transport by UCP1 -- 10. Role of cAMP and cGMP Signaling in Brown Fat -- 11. Fatty Acid Metabolites as Novel Regulators of Non-shivering Thermogenesis -- 12. Regulatory Small and Long Noncoding RNAs in Brite/Brown Adipose Tissue -- 13. Brown Adipokines -- Part 3 Detection of BAT in vivo -- 14. Infrared Thermography -- 15. In Vivo Detection of Human Brown Adipose Tissue During Cold and Exercise by PET/CT -- 16. Techniques and Applications of Magnetic Resonance Imaging for Studying Brown Adipose Tissue Morphometry and Function -- 17. Multispectral Optoacoustic Tomography of Brown Adipose Tissue -- 18. BAT Exosomes: Metabolic Crosstalk with Other Organs and Biomarkers for BAT Activity -- Part 4. Recruitment and Activation of human BAT -- 19. Activation of Human Brown Adipose Tissue (BAT): Focus on Nutrition and Eating -- 20. Translational Aspects of Brown Fat Activation by Food-Derived Stimulants -- 21. Translational Pharmacology and Physiology of Brown Adipose Tissue in Human Disease and Treatment
    Abstract: The main focus of this book is on brown adipose tissue and its metabolic function. The book provides a timely update on the latest research and shows where the field is heading. Brown adipose tissue (BAT) dissipates energy and has received considerable attention in the last few years, having been re-discovered in adult humans in 2007/9. Moreover, BAT might offer a target for novel therapies to address obesity, a health condition that has reached pandemic dimensions
    Pages: VIII, 424 p. 52 illus., 49 illus. in color. : online resource.
    ISBN: 9783030105136
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  • 30
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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  • 31
    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 32
    facet.materialart.
    Singapur : Springer
    Pages: xvi, 367 p.
    ISBN: 9789811319419
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  • 33
    Keywords: Stem Cells ; Oncology ; Cell Cycle ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Cell Cycle Analysis ; Springer eBooks
    Description / Table of Contents: Stem cells heterogeneity -- Heterogeneity in epiblast stem cells -- Progenitor cell heterogeneity in the adult carotid body germinal niche -- Planarian stem cell heterogeneity -- The cellular origin of Barrett’s esophagus and its stem cells -- Pluripotent stem cell heterogeneity -- Sarcoma stem cell heterogeneity -- Heterogeinity in adipose derived stem cells -- Unveiling stem cell heterogeneity towards the development of salivary gland regenerative strategies -- Heterogeneity of human mesenchymal stromal/stem cells -- Mitochondrial heterogeneity in stem cells -- The heterogeneity of renal stem cells, and their interaction with bio- and nano-materials -- Index
    Abstract: This book presents a comprehensive discussion on the novel concepts in stem cell heterogeneity, from pluripotent stem cells to human mesenchymal stem cells, adult and cancer stem cells of the thyroid, sarcoma, and more. Thus, Stem Cell Heterogeneity - Novel Concepts starts from a timely update on the current information on stem cells heterogeneity in various tissues and discusses new concepts and future directions. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity in Different Organs, and Stem Cells Heterogeneity in Cancer, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians
    Pages: XII, 225 p. 45 illus., 38 illus. in color. : online resource.
    ISBN: 9783030110963
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  • 34
    Keywords: Human Physiology ; Neurosciences ; Cytology ; Radiology, Medical ; Stem Cells ; Nanotechnology ; Human Physiology ; Neurosciences ; Oxidative Stress ; Diagnostic Radiology ; Stem Cells ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Cerebral stroke: An Introduction -- Chapter 2. Inflammation, oxidative stress, and neurodegeneration -- Chapter 3. Stroke induced blood brain barrier damage -- Chapter 4. Regulation of calcium ions in ischemic neuronal cell -- Chapter 5. Ischemic stroke induced endoplasmic reticulum stress -- Chapter 6. The role of autophagy in ischemic stroke: friend or foe?- Chapter 7. Critical role of mitochondrial autophagy in cerebral stroke -- Chapter 8. Application of neuroimaging in the identification of the pinpoint location of blockage -- Chapter 9. Emerging role of the electromagnetic field in stroke -- Chapter 10. Stem cell therapies for stroke -- Chapter 11. MicroRNA: Significance to stroke diagnosis, prognosis, and therapy -- Chapter 12. Therapeutic Aspects of Nanomedicines in Stroke Treatment -- Chapter 13. Neuroprotective potential of small molecule phytochemicals against stroke -- Chapter 14. Role of UV irradiation on neuroprotective potential of phytochemicals -- Chapter 15. Post-stroke treatment strategies, management, and rehabilitation
    Abstract: This book provides detailed and comprehensive mechanistic insights of the various risk factors that lead to the ischemic stroke and the novel therapeutic interventions against it. The first section discusses the different ischemic cerebral stroke-induced inflammatory pathways and dysfunctionality of blood-brain barrier. The later sections of the book deals with the role of endoplasmic reticulum stress and mitophagy in cerebral stroke and introduces the different neuroimaging techniques such as Computed tomography (CT), Magnetic resonance imaging (MRI), Positron emission tomography (PET) and Single-Photon emission computed tomography (SPECT) that are used to identify the arterial blockages. The final section comprises of chapters that focus on various neuroprotective strategies and emerging therapeutic interventions for combating stroke pathophysiology. The chapters cover the role of stem cell therapy, the therapeutic effect of low-frequency electromagnetic radiations (LF-EMR), and implications of non-coding RNAs such as micro-RNAs as the biomarkers for diagnosis, prognosis, and therapy in ischemic stroke
    Pages: VIII, 189 p. 23 illus., 12 illus. in color. : online resource.
    ISBN: 9789811314537
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  • 35
    Keywords: Microbiology ; Medicine ; Emerging infectious diseases ; Medical Microbiology ; Health Promotion and Disease Prevention ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Diagnostics, Therapeutics and Other Potential Strategies of Biodefense -- Advanced Molecular Biology Tools & Diagnostics -- High Containment Infectious Disease Laboratories as an Integral Part of Emergency Preparedness -- Decontamination Capabilities and Facilities -- Virogenomics as a Tool for Molecular Epidemiology and Forensics -- Next Generation Sequencing Tools for Pathogen Detection -- Metagenomics -- Point-of-care Diagnostic Facilities for Outbreak Intervention -- Psychological Impact of Biological Attacks -- Public-health Preparedness -- Viral Therapeutics -- Bacterial Therapeutics -- Emergency Training for Veterinary Professionals -- Global Biodefense Coordination
    Abstract: This second volume of a two-volume set focuses on specific pathogens and their mechanisms of pathogenesis as well as diagnostics, therapeutics and various strategies in the event of biological attacks. This multidisciplinary book appeals to readers from various fields, including biodefense, biosafety & biosecurity, virology, neurology, molecular biology and genetic engineering, as well as infectious disease specialists. Further, the work is of interest to basic science and applied science research scholars and experts working in the area of high-consequence or select agent virology
    Pages: XIII, 383 p. 30 illus., 25 illus. in color. : online resource.
    ISBN: 9783030030711
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  • 36
    Keywords: Pharmaceutical technology ; Analytical biochemistry ; Biochemistry ; Pharmaceutical Sciences/Technology ; Analytical Chemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recognition Mechanisms of Chiral Selectors: An Overview -- Enantioseparation by Thin Layer Chromatography -- Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase -- Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS -- Chiral Mobile Phase Additives in HPLC Enantioseparations -- Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High Performance Liquid Chromatography: An Overview -- HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions -- Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC -- HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases -- Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector -- Cyclofructans as Chiral Selectors: An Overview -- High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview -- Application of Sub-2 Micron Particles Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography -- Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview -- Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand-Exchange -- Applications of Chiral Supercritical Fluid Chromatography -- Chiral Separations by Countercurrent Chromatography -- Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations -- Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations -- Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer -- Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins -- Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes -- Chiral Capillary Electrophoresis-Mass Spectrometry -- Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography -- Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry -- Application of a (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography -- Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview
    Abstract: This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry
    Pages: XIV, 487 p. 147 illus., 57 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493994380
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  • 37
    Keywords: Toxicology ; Embryology ; Cytology ; Pharmacology/Toxicology ; Embryology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vitro Models in Developmental Toxicology -- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments -- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology -- Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model -- Micromass Methods for the Evaluation of Developmental Toxicants -- The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System -- Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology -- Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology -- Hepatotoxicity in Zebrafish Larvae -- Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model -- The Chicken Embryo as a Model in Developmental Toxicology -- The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity -- Mouse Whole Embryo Culture -- Rat Whole Embryo Culture -- Rabbit Whole Embryo Culture -- Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology -- Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish -- Assessment of Histiotrophic Nutrition Using Fluorescent Probes -- Examining Gene Expression Patterns through Whole Mount In Situ Hybridization -- Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos -- Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection -- Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA -- Western Analysis of Breast Cancer 1 Protein (BRCA1) -- Regulation and Control of AP-1 Binding Activity in Embryotoxicity -- Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) -- The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity -- Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining
    Abstract: This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures
    Pages: XI, 436 p. 155 illus., 104 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991822
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  • 38
    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 39
    Keywords: Cytology ; Mass spectrometry ; Monoclonal antibodies ; Cell Biology ; Mass spectrometry ; Antibodies ; Springer eBooks
    Description / Table of Contents: Setting up Mass Cytometry in a Shared Resource Lab Environment -- Acquisition, Processing, and Quality Control of Mass Cytometry Data -- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating -- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments -- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis -- Titration of Mass Cytometry Reagents -- Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry -- Automated Cell Processing for Mass Cytometry Experiments -- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry -- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry -- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry -- Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry -- Mass Cytometric Cell Cycle Analysis -- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry -- Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry -- Data-Driven Flow Cytometry Analysis -- Analysis of Mass Cytometry Data -- Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry -- Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples -- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery
    Abstract: This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 338 p. 83 illus., 70 illus. in color. : online resource.
    ISBN: 9781493994540
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  • 40
    facet.materialart.
    New York, NY : Springer
    Keywords: Neurosciences ; Cell Culture ; Neurosciences ; Cell Culture ; Springer eBooks
    Description / Table of Contents: In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model -- In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models -- Oxidative Stress Signatures in Human Stem Cell Derived Neurons -- Glial Reactivity in Response to Neurotoxins: Relevance and Methods -- Neuron-Glia Interaction Studied with In Vitro Co-Culture -- Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria -- Epigenetic Changes in Cultured Neurons and Astrocytes -- The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation -- Zebrafish as a Tool to Assess Developmental Neurotoxicity -- Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies -- Electrophysiological Neuromethodologies -- A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies -- Transporter Studies: Brain Punching Technique -- MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders -- Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes
    Abstract: This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field
    Pages: XIII, 302 p. 57 illus., 47 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992287
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  • 41
    Keywords: Biochemistry ; Mass spectrometry ; Protein Science ; Mass spectrometry ; Springer eBooks
    Description / Table of Contents: Next Generation Proteomics for Clinical Biomarker Detection using SWATH-MS -- A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification of Protein Ubiquitination -- Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometry -- Quantitative Phosphoproteomic using Titanium Dioxide Micro-Columns and Label Free Quantitation -- Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond -- Quantitative Analysis of Protein S-Acylation Site Dynamics Using Site-Specific Acyl-Biotin Exchange (ssABE) -- Reducing Complexity? Cysteine Reduction and S-alkylation in Proteomic Workflows: Practical Considerations -- Detection of Unknown Chemical Adduct Modifications on Proteins: From Wet to Dry Laboratory -- Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry -- Metaproteomics of Freshwater Microbial Communities -- Peptide-to-Protein Summarization: An Important Step for Accurate Quantification In Label-Based Proteomics -- Experimental Design in Quantitative Proteomics -- Practical Integration of Multi-Run iTRAQ Data -- Quantitative Proteomics Data in the Public Domain: Challenges and Opportunities -- Computational Proteomics with Jupyter and Python -- The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis
    Abstract: New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIII, 264 p. 54 illus., 35 illus. in color. : online resource.
    ISBN: 9781493992324
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  • 42
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Making the Case for Functional Proteomics -- Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors -- Two-dimensional 16-BAC/SDS polyacrylamide gel electrophoresis of mitochondrial membrane proteins -- Systematic Glycolytic Enzyme Activity Analysis from Human Serum with PEP Technology -- A protein decomplexation strategy in snake venom proteomics -- Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level -- A systematic analysis workflow for high-density customized protein microarrays in biomarker screening -- Metaproteomics study of the gut microbiome -- Double-One Dimensional Electrophoresis (D1-DE) Adapted for Immunoproteomics -- BioID: A proximity dependent labeling approach in proteomics study -- Functional application of snake venom proteomics in in vivo antivenom assessment -- Proteomic detection of carbohydrate-active enzymes (CAZymes) in microbial secretomes -- An Overview of Mass Spectrometry-based Methods for Functional Proteomics -- Functional proteomic analysis to characterize signaling crosstalks -- Identification of unexpected protein modifications by mass spectrometry based proteomics -- Label-free LC-MS/MS Strategy for Comprehensive Proteomic Profiling of Human Islets Collected using Laser Capture Microdissection from Frozen Pancreata -- Mass Spectrometry based targeted proteomics for cell and tissue analysis -- Metabolomic investigation of Staphylococcus aureus antibiotic susceptibility by liquid chromatography coupled to high resolution mass spectrometry -- Nuts and bolts of protein quantification by on-line trypsin digestion coupled LC-MS/MS analysis -- Proteases: Pivot Points in Functional Proteomics -- The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics -- Efficient Extraction and Digestion of Gluten Proteins -- Glycosylation profiling of tumor marker in plasma using bead-based immunoassay -- Protein-specific analysis of invertebrate glycoproteins -- The use of proteomics studies in identifying moonlighting proteins -- Two-dimensional biochemical purification for global proteomic analysis of macromolecular protein complexes -- A data analysis protocol for quantitative data-independent acquisition proteomics
    Abstract: This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed
    Pages: XII, 477 p. 89 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988143
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  • 43
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Phytoplasmas: An Introduction -- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques -- Phytoplasma Transmission: Insect Rearing and Infection Protocols -- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors -- Symptoms of Phytoplasma Diseases -- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas -- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene -- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas -- Real-time PCR Protocol for Phytoplasma Detection and Quantification -- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves -- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas -- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas -- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification -- Quantitative Analysis with Droplet Digital PCR -- Rapid Sample Preparation and LAMP for Phytoplasma Detection -- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly -- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’ -- Transcriptomic Analyses of Phytoplasmas -- Transcriptomic Analyses of Phytoplasmas -- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study -- Collection of Phloem Sap in Phytoplasma-infected Plants -- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas -- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas -- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) -- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants -- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants
    Abstract: This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 362 p. 75 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988372
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  • 44
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinsoń€™s Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- ℗Ư℗ƯMonitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the €œáutophagic flux́€ in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations
    Abstract: This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XVII, 741 p. 137 illus., 99 illus. in color. : online resource.
    ISBN: 9781493988730
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  • 45
    Keywords: Gene Therapy ; Oncology ; Medicine ; Gene Therapy ; Cancer Research ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Origins of Suicide Gene Therapy -- Cancer Suicide Gene Therapy with TK.007 -- Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer -- Suicide Gene Therapy for Oral Squamous Cell Carcinoma -- Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy -- Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer -- Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy -- Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells -- Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45α to Arrest the G2/M Transition -- Non-Invasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging -- In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector -- Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells -- Photochemical Internalization Enhanced Non-Viral Suicide Gene Therapy -- Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro
    Abstract: This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy
    Pages: XII, 190 p. 37 illus., 26 illus. in color. : online resource.
    ISBN: 9781493989225
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  • 46
    Keywords: Production of electric energy or ; Systems engineering ; Electronics ; Power Electronics, Electrical Machines and Networks ; Circuits and Systems ; Energy Systems ; Electronics and Microelectronics, Instrumentation ; Springer eBooks
    Description / Table of Contents: Introduction -- A General Control Strategy for DC-DC Series-Parallel Power Conversion Systems -- Mathematical Model of DC-DC ISOP System and Closed-Loop Parameters Design -- Wireless IVS Control Strategies for Input-Series-Connected Systems Based on Positive Output Voltage Gradient Method -- General Control Strategy for DC-AC Inverter Series-Parallel Combined System -- Compound Balanced Control Strategy for Input-Series-Output-Parallel DC-AC Inverter System -- Compound Balanced Control Strategy for Input-Series-Output-Series DC-AC Inverter System -- An Improved Average Current Control Strategy for Input-Parallel-Output-Parallel Inverter System -- Input Voltage Sharing Control Strategy for ISOP Systems under Extreme Load Conditions
    Abstract: Series-parallel conversion systems, in which multiple standardized converter modules are connected in series or parallel at the input and output sides, to meet the demands of various applications. This book focuses on the control strategies for the series-parallel conversion systems with DC-DC converters and DC-AC inverters as the basic modules, respectively, to achieve input voltage/current sharing and output voltage/current sharing among the constituent modules. The detailed theoretical analysis with design examples and experimental validations are presented. This book is essential and valuable reference for graduate students and academics majoring in power electronics and engineers engaged in developing DC-DC converters, DC-AC inverters and power electronics transformers
    Pages: XX, 213 p. 156 illus., 74 illus. in color. : online resource.
    ISBN: 9789811327605
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  • 47
    Keywords: Stem Cells ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells -- Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver -- Identification and Isolation of Clonogenic Cholangiocyte in Mouse -- Isolation and Expansion of Rat Hepatocytic Progenitor Cells -- Genetic Lineage Tracing of Biliary Epithelial Cells -- Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination -- Analysis for the Heterogeneity of Liver Progenitor Cells -- Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration -- Conversion of Fibroblasts to Hepatocytes In Vitro -- Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo -- Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells -- Induction of Functional Hepatocytes from Human iPSCs -- Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells -- Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture -- Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device -- Generation of Hepatic Organoids with Biliary Structures -- Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration -- Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells -- A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells -- Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes -- Mouse Model for Cholangiocarcinoma from Peribiliary Glands
    Abstract: This volume looks at the liveŕ€™s epithelial cellś€”hepatocytes and cholangiocytes€”ánd their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology
    Pages: XI, 248 p. 70 illus., 59 illus. in color. : online resource.
    ISBN: 9781493989614
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  • 48
    Keywords: Biotechnology ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Gene Regulatory Network Inference: An Introductory Survey -- Statistical Network Inference for Time-Varying Molecular Data with Dynamic Bayesian Networks -- Overview and Evaluation of Recent Methods for Statistical Inference of Gene Regulatory Networks from Time Series Data -- Whole-Transcriptome Causal Network Inference with Genomic and Transcriptomic Data -- Causal Queries from Observational Data in Biological Systems via Bayesian Networks: An Empirical Study in Small Networks -- A Multiattribute Gaussian Graphical Model for Inferring Multiscale Regulatory Networks: An Application in Breast Cancer -- Integrative Approaches for Inference of Genome-Scale Gene Regulatory Networks -- Unsupervised Gene Network Inference with Decision Trees and Random Forests -- Tree-Based Learning of Regulatory Network Topologies and Dynamics with Jump3 -- Network Inference from Single-Cell Transcriptomic Data -- Inferring Gene Regulatory Networks from Multiple Datasets -- Unsupervised GRN Ensemble -- Learning Differential Module Networks across Multiple Experimental Conditions -- Stability in GRN Inference -- Gene Regulatory Networks: A Primer in Biological Processes and Statistical Modelling -- Scalable Inference of Ordinary Differential Equation Models of Biochemical Processes
    Abstract: This volume explores recent techniques for the computational inference of gene regulatory networks (GRNs). The chapters in this book cover topics such as methods to infer GRNs from time-varying data; the extraction of causal information from biological data; GRN inference from multiple heterogeneous data sets; non-parametric and hybrid statistical methods; the joint inference of differential networks; and mechanistic models of gene regulation dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, descriptions of recently developed methods for GRN inference, applications of these methods on real and/ or simulated biological data, and step-by-step tutorials on the usage of associated software tools. Cutting-edge and thorough, Gene Regulatory Networks: Methods and Protocols is an essential tool for evaluating the current research needed to further address the common challenges faced by specialists in this field
    Pages: XI, 430 p. 114 illus., 71 illus. in color. : online resource.
    ISBN: 9781493988822
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  • 49
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Characterization of Formulations for Freeze Drying -- Formulation and Process Development for Lyophilized Biological Reference Materials -- Controlled Ice Nucleation Using ControLyo® Pressurization-Depressurization Method -- Alternative Methods of Controlling Nucleation in Freeze Drying -- Tunable Diode Laser Absorption Spectroscopy in Lyophilization -- Containment Options for the Freeze-Drying of Biological Entities and Potent Materials -- Freeze Drying Systems: Freeze Dryer Design Requirements & Automatic Loading and Unloading Systems (ALUS™) -- Regulatory Aspects of Freeze Drying -- Container and Reconstitution Systems for Lyophilized Drug Products -- Scale-Up of Freeze-Drying Cycles, the Use of Process Analytical Technology (PAT) and Statistical Analysis -- Through Vial Impedance Spectroscopy (TVIS): A Novel Approach to Process Understanding for Freeze-Drying Cycle Development -- Lyophilization of High-Concentration Protein Formulations -- Mechanical Behavior and Structure of Freeze Dried Cakes -- High Resolution Mass Spectrometric Methods for Proteins in Lyophilized Solids
    Abstract: This detailed volume brings together leading practitioners in the freeze-drying community to address recent progress, not only in new analytical tools and applications of the data derived in cycle design but also in the manufacturing of lyophilized products in the healthcare sector – whether these be therapeutics, vaccines or diagnostic products - and indeed the equipment to deliver this scale of freeze-drying. Areas of focus include analytical and formulation issues, process monitoring and control, as well as post-lyophilization analysis. Written for the Methods in Pharmacology and Toxicology series, chapters include the type of expert advice that leads to superior results in the lab. Authoritative and practical, Lyophilization of Pharmaceuticals and Biologicals: New Technologies and Approaches serves as an ideal guide for researchers working in or just seeking an update on this rapidly changing field
    Pages: XIV, 382 p. 188 illus., 148 illus. in color. : online resource.
    ISBN: 9781493989287
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  • 50
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    New York, NY : Springer
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: In Vitro Methods for Assessing Nanoparticle Toxicity -- Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure -- Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment -- Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing -- Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles -- In Vitro Approaches for Assessing the Genotoxicity of Nanomaterials -- Measurement of Nanoparticle Induced Mitochondrial Membrane Potential Alterations -- Application of Gelatin Zymography in Nanotoxicity Research -- Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies -- Analysis of Nanomaterial Toxicity by Western Blot -- Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR -- DNA Methylation Analysis -- Evaluation of Nrf2 with Exposure to Nanoparticles -- Application of Electron Microscopes in Nanotoxicity Assessment -- Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry -- Methods to Quantify Nanomaterial Association with, and Distribution Across, the Blood-Brain Barrier In Vivo -- Evaluation of Genotoxicity of Nanoparticles in Mouse Models -- Evaluation of Pulmonary Toxicity of Nanoparticles by Bronchoalveolar Lavage -- Pulmonary Function Testing in Animals -- Nanotoxicity Assessment Using Embryonic Zebrafish -- Application of Daphnia magna for Nanoecotoxicity Study -- The Overview of Methods of Nanoparticle Exposure Assessment
    Abstract: This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field
    Pages: XII, 371 p. 68 illus., 52 illus. in color. : online resource.
    ISBN: 9781493989164
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  • 51
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: 1. Isolation and identification of lactic acid bacteria from environmental samples -- 2. Isolation and identification of lactic acid bacteria from environmental samples -- 3. Isolation and identification of lactic acid bacteria from environmental samples -- 4. Isolation and identification of lactic acid bacteria from environmental samples -- 5. Isolation and identification of lactic acid bacteria from environmental samples -- 6. Isolation and identification of lactic acid bacteria from environmental samples -- 7. Isolation and identification of lactic acid bacteria from environmental samples -- 8. Isolation and identification of lactic acid bacteria from environmental samples -- 9. Isolation and identification of lactic acid bacteria from environmental samples -- 10. Isolation and identification of lactic acid bacteria from environmental samples -- 11. Isolation and identification of lactic acid bacteria from environmental samples -- 12. Isolation and identification of lactic acid bacteria from environmental samples -- 13. Isolation and identification of lactic acid bacteria from environmental samples -- 14. Isolation and identification of lactic acid bacteria from environmental samples -- 15. Isolation and identification of lactic acid bacteria from environmental samples -- 16. Isolation and identification of lactic acid bacteria from environmental samples
    Abstract: This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science
    Pages: X, 194 p. 58 illus., 10 illus. in color. : online resource.
    ISBN: 9781493989072
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  • 52
    facet.materialart.
    New York, NY : Springer
    Keywords: Medicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Traditional Prenatal Diagnosis: Past to Present -- Overview of Preimplantation Genetic Diagnosis (PGD): Historical Perspective and Future Direction -- Non-Invasive Approaches to Prenatal Diagnosis: Historical Perspective and Future Directions -- Molecular Testing for Preimplantation Genetic Diagnosis of Single Gene Disorders -- Detection of Aneuploidy and Unbalanced Rearrangements using Comparative Genomic Hybridization Microarrays -- Aneuploidy Screening using Next Generation Sequencing -- DNA Extraction from Various Types of Prenatal Specimens -- Assessment of Maternal Cell Contamination in Prenatal Samples by Quantitative Fluorescent PCR (QF-PCR) -- Rapid Prenatal Aneuploidy Screening by Fluorescence In Situ Hybridization (FISH) -- Prenatal Detection of Chromosome Aneuploidy by Quantitative Fluorescence PCR -- Multiplex Ligation-Dependent Probe Amplification (MLPA) for Prenatal Diagnosis of Common Aneuploidies -- Chromosomal Microarray Analysis using Array Comparative Genomic Hybridization on DNA from Amniotic Fluid and Chorionic Villus Sampling -- Prenatal Diagnosis using Chromosomal SNP Microarrays -- Rapid Detection of Fetal Mendelian Disorders: Thalassemia and Sickle Cell Syndromes -- Prenatal Diagnosis of Cystic Fibrosis -- Prenatal Diagnosis of Tay-Sachs Disease -- Next-Generation Sequencing of Prenatal Structural Chormosomal Rearrangements using Large-Insert Libraries -- Prenatal Diagnosis by Whole Exome Sequencing in Fetuses with Ultrasound Abnormalities -- Quad Screen Test, A Multiplexed Biomarker Assay for Prenatal Screening to Assess Birth Defects: The Columbia University Experience using the Beckman Access2 Immunoassay Analyzer and Benetech PRA -- Isolation of Cell-Free DNA from Maternal Plasma -- Noninvasive Detection of Fetal Aneuploidy using Next-Generation Sequencing -- Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis
    Abstract: This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this book are divided into three sections: preimplantation genetic testing, traditional prenatal testing, and non-invasive prenatal testing. This book covers topics such as molecular testing for preimplantation genetic diagnosis of single gene disorders; DNA extraction from va