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  • Springer  (1,652,002)
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  • 1
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 2
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    Cham : Springer
    Keywords: Immunology ; Emerging infectious diseases ; Immunology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: 1. Epidemiology of extrapulmonary tuberculosis -- 2. Pleural Tuberculosis -- 3. Gastrointestinal Tuberculosis -- 4. Hepatobiliary and splenic tuberculosis -- 5. Lymphatic Tuberculosis -- 6. Bone and Joint Tuberculosis -- 7. Tuberculous Spondylodiscitis -- 8. Tuberculous Meningitis -- 9. Tuberculous Encephalitis -- 10. Spinal tuberculosis -- 11. Genitourinary Tuberculosis -- 12. Cardiovascular Tuberculosis -- 13. Cutaneous Tuberculosis -- 14. Tuberculosis of Eye -- 15. Infection control in extrapulmonary TB -- 16. Tuberculosis in the ICU
    Abstract: This book brings awareness to a neglected condition that is nevertheless prevalent world-wide. Much focus is justly given to pulmonary tuberculosis, one of the key medical scourges of humanity, but this disease also often manifests itself in organs outside of the lungs. There is however a surprising lack of information available on extrapulmonary TB, which this book aims to remedy. Specifically, research, as well as epidemiology, diagnosis, and treatment options, are discussed in detail by an international list of experts. This comprehensive product serves as a valuable resource to numerous fields of medicine due to the presence of extrapulmonary tuberculosis throughout the human body
    Pages: VIII, 208 p. 7 illus., 1 illus. in color. : online resource.
    ISBN: 9783030047443
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  • 3
    Keywords: Microbiology ; Biomedical Engineering ; Microbial ecology ; Microbiology ; Biomedical Engineering/Biotechnology ; Microbial ecology ; Applied Microbiology ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: Chapter 1. The Dawn of Novel Biotechnological Applications of Polyhydroxyalkanoates -- Chapter 2. Strategy for biosynthesis of polyhydroxyalkonates polymers/copolymers and their application in drug delivery -- Chapter 3. Applications of Polyhydroxyalkanoates and its metabolites as transporters for bioactive molecules -- Chapter 4. Polyhydroxyalkanoates applications in antimicrobial agents' delivery and wound healing -- Chapter 5. Polyhydroxyalkanoates applications in Drug Carriers -- Chapter 6. Applications of polyhydroxyalkanoate based nanovehicles -- Chapter 7. Memory enhancers -- Chapter 8. Novel Biocontrol Agents: Short Chain Fatty Acids And More Recently, Polyhydroxyalkanoates -- Chapter 9. Potential Therapeutic Applications of Polyhydroalkanoates and Their Composites as Anti-microbial Agents -- Chapter 10. Polyhydroxyalkanoates (PHA) – Applications in Wound Treatment and as Precursors for Oral Drugs -- Chapter 11. Exploiting Polyhydroxyalkanoates for Tissue Engineering -- Chapter 12. CH4-based polyhydroxyalkanoate production: a step further towards a sustainable bioeconomy -- Chapter 13. Applications of PHA in Agriculture -- Chapter 14. Polyhydroxyalkanoates in Packaging -- Chapter 15. Approaches for Enhancing Extraction of bacterial polyhydroxyalkanoates for Industrial Applications -- Chapter 16. Nanofibers from polyhydroxyalkanoates and their applications in tissue engineering
    Abstract: This book presents the latest research on the uses of polyhydroxyalkanoates (PHA), introducing readers to these natural, biodegradable polyesters produced by microorganisms, their functions and applications. The individual chapters discuss the various potentials of these bioplastics, which offer an attractive alternative to non-biodegradable plastics. The book also describes the diverse medical and biomedical applications of PHAs, including their use as drug carriers, memory enhancers, and biocontrol agents, and examines their role in creating a more sustainable economy – which is the need of the hour
    Pages: XII, 420 p. 42 illus., 34 illus. in color. : online resource.
    ISBN: 9789811337598
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  • 4
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- A Neuron-Glial perspective for computational neuroscience -- Part 2. Calcium dynamics -- Data-driven modelling of the inositol trisphosphate receptor and its role in calcium induced calcium release -- Intracellular calcium dynamics: biophysical and simplified models -- Modeling of stochastic Ca2+ signals -- G protein-coupled receptor-mediated astrocytic Ca2+ signaling in astrocytes -- Emergence of regular and complex calcium oscillations by inositol 1,4,5-trisphosphate signaling in astrocytes -- Astrocyte networks and intercellular calcium propagation -- Part 3. Tripartite Synapse and regulation of network activity -- Tripartite synapses and regulation of synaptic plasticity -- Purinergic signaling at tripartite synapses -- Gliotransmitter exocytosis and its consequences on synaptic transmission -- Computational models of pathophysiological glial activation in CNS disorders -- Part 4. Homeostasis and Metabolic coupling -- The role of astrocytes in neurotransmitter uptake and brain metabolism -- Glutamate uptake -- Astrocytic ion dynamics: Implications for potassium buffering and liquid flow -- Constraint-Based Modeling of Metabolic Interactions in and between Astrocytes and Neurons -- Part 5. Computational Tools to Analyze and Model Astrocyte Experiments -- Computational models of astrocytes and astrocyte-neuron interactions: Characterization, reproducibility, and future perspectives -- GECIquant: semi-automated detection and quantification of astrocyte intracellular Ca2+ signals monitored with GCaMP6f -- Modeling neuron-glia interactions with the Brian 2.0 simulator
    Abstract: Over the last two decades, the recognition that astrocytes - the predominant type of cortical glial cells - could sense neighboring neuronal activity and release neuroactive agents, has been instrumental in the uncovering of many roles that these cells could play in brain processing and the storage of information. These findings initiated a conceptual revolution that leads to rethinking how brain communication works since they imply that information travels and is processed not just in the neuronal circuitry but in an expanded neuron-glial network. On the other hand the physiological need for astrocyte signaling in brain information processing and the modes of action of these cells in computational tasks remain largely undefined. This is due, to a large extent, both to the lack of conclusive experimental evidence, and to a substantial lack of a theoretical framework to address modeling and characterization of the many possible astrocyte functions. This book that we propose aims at filling this gap, providing the first systematic computational approach to the complex, wide subject of neuron-glia interactions. The organization of the book is unique insofar as it considers a selection of ́€œhot topicś€ in glia research that ideally brings together both the novelty of the recent experimental findings in the field and the modelling challenge that they bear. A chapter written by experimentalists, possibly in collaboration with theoreticians, will introduce each topic. The aim of this chapter, that we foresee less technical in its style than in conventional reviews, will be to provide a review as clear as possible, of what is €œéstablished́€ and what remains speculative (i.e. the open questions). Each topic will then be presented in its possible different aspects, by 2-3 chapters by theoreticians. These chapters will be edited in order to provide a ́€œpriminǵ€ reference for modeling neuron-glia interactions, suitable both for the graduate student and the professional researcher
    Pages: XV, 505 p. 205 illus., 76 illus. in color. : online resource.
    ISBN: 9783030008178
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  • 5
    Keywords: Ecology ; Evolution (Biology) ; Plant Ecology ; Animal ecology ; Plant genetics ; Biodiversity ; Community & Population Ecology ; Evolutionary Biology ; Plant Ecology ; Animal ecology ; Plant Genetics and Genomics ; Biodiversity ; Springer eBooks
    Description / Table of Contents: Part1. Concepts, Technical Advances and Challenges -- Chapter1. Technical Advances and Challenges in Genome-Scale Analysis of Ancient DNA -- Chapter2. Paleoproteomics: An Introduction to the Analysis of Ancient Proteins by Soft Ionization Mass Spectrometry -- Chapter3. Ancient RNA -- Chapter4. Ancient Epigenomics -- Part2. -- Chapter5. Ancient Pathogens Through Human History: A Paleogenomic Perspective -- Chapter6. Paleovirology: Viral Sequences from Historical and Ancient DNA -- Chapter7. Reconstructing Past Vegetation Communities Using Ancient DNA from Lake Sediments -- Chapter8. Archaeogenomics and Crop Adaptation -- Chapter9. Herbarium Genomics: Plant Archival DNA Explored -- Chapter10. Paleogenomics of Animal Domestication -- Chapter11. Paleogenomic Inferences of Dog Domestication -- Chapter12. Of Cats and Men: Ancient DNA Reveals How the Cat Conquered the Ancient World -- Chapter13. An Ancient DNA Perspective on Horse Evolution -- Chapter14. Primate Paleogenomics -- Chapter15. Structural Variants in Ancient Genomes -- Chapter16. Genomics of Extinction
    Abstract: Advances in genome-scale DNA sequencing technologies have revolutionized genetic research on ancient organisms, extinct species, and past environments. When it is recoverable after hundreds or thousands of years of unintended preservation, €œáncient DNÁ€ (or aDNA) is often highly degraded, necessitating specialized handling and analytical approaches. Paleogenomics defines the field of reconstructing and analyzing the genomes of historic or long-dead organisms, most often through comparison with modern representatives of the same or similar species. The opportunity to isolate and study paleogenomes has radically transformed many fields, spanning biology, anthropology, agriculture, and medicine. Examples include understanding evolutionary relationships of extinct species known only from fossils, the domestication of plants and animals, and the evolution and geographical spread of certain pathogens. This pioneering book presents a snapshot view of the history, current status, and future prospects of paleogenomics, taking a broad viewpoint that covers a range of topics and organisms to provide an up-to-date status of the applications, challenges, and promise of the field. This book is intended for a variety of readerships, including upper-level undergraduate and graduate students, professionals and experts in the field, as well as anyone excited by the extraordinary insights that paleogenomics offers
    Pages: XIV, 427 p. 62 illus., 57 illus. in color. : online resource.
    ISBN: 9783030047535
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  • 6
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: A Homozygous Splice Site Mutation in SLC25A42, Encoding the Mitochondrial Transporter of Coenzyme A, Causes Metabolic Crises and Epileptic Encephalopathy -- Apparent Acetaminophen Toxicity in a Patient with Transaldolase Deficiency -- Sialuria: Ninth Patient Described Has a Novel Mutation in GNE -- Stability of the ABCD1 Protein with a Missense Mutation: A Novel Approach to Finding Therapeutic Compounds for X-Linked Adrenoleukodystrophy -- Psychosocial Functioning in Parents of MPS III Patients -- The Second Case of Saposin A Deficiency and Altered Autophagy -- An Electronic Questionnaire for Liver Assessment in Congenital Disorders of Glycosylation (LeQCDG): A Patient-Centered Study -- Demographics, Clinical Features, and Mortality of Acute Hepatic Porphyrias in Colombia: An Analysis of 101 Patients -- Cobalamin D Deficiency Identified Through Newborn Screening -- Lathosterolosis: A Relatively Mild Case with Cataracts and Learning Difficulties -- DPAGT1 Deficiency with Encephalopathy (DPAGT1-CDG): Clinical and Genetic Description of 11 New Patients -- Enzyme Replacement Therapy During Pregnancy in Fabry Patients -- Hyperornithinemia, Hyperammonemia, and Homocitrullinuria Syndrome Causing Severe Neonatal Hyperammonemia -- Screening for Niemann-Pick Type C Disease in a Memory Clinic Cohort -- Reversible Cerebral White Matter Abnormalities in Homocystinuria
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 119 p. 32 illus., 22 illus. in color. : online resource.
    ISBN: 9783662586174
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  • 7
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels -- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling -- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators -- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes -- MCU Regulation in Lipid Bilayer and Electrophysiological Recording -- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer -- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter -- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana -- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes -- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes -- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle -- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis -- Ca2+-Dependent Regulation of TFEB and Lysosomal Function -- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum -- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2 -- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes -- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes -- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer -- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells -- Purification of Functional F-ATP Synthase from Blue Native PAGE
    Abstract: This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research
    Pages: XIII, 246 p. 48 illus., 39 illus. in color. : online resource.
    ISBN: 9781493990184
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  • 8
    Keywords: Neurosciences ; Stem Cells ; Laboratory animals ; Neurosciences ; Stem Cells ; Animal Models ; Springer eBooks
    Description / Table of Contents: Generation of Neural Stem Cells from Induced Pluripotent Stem Cells -- Making NSC and Neurons from Patient Derived Tissue Samples -- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury -- Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells -- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells -- In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology -- Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons -- Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene -- Single-Cell Library Preparation of iPSC-derived Neural Stem Cells -- Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells -- Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons -- Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach -- Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells -- Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation -- Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart -- Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion
    Abstract: This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIV, 239 p. 51 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990078
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  • 9
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: A Genomic View of the Catabolism of Aromatic Compounds in Pseudomonas -- A Genomic View of the Catabolism of Short Chain Alkanes -- Aerobic Degradation of Aromatic Hydrocarbons -- Aerobic Degradation of Aromatic Hydrocarbons: Enzyme Structures and Catalytic Mechanisms -- Aerobic Degradation of Chloroaromatics -- Aerobic Degradation of Gasoline Ether Oxygenates -- Aerobic Degradation of Halogenated Aliphatics -- Biochemistry and Molecular Biology of Methane Monooxygenase -- Biosynthesis, Insertion and Function of Fe-S Cofactors -- Biosynthesis, Insertion and Function of Flavin Cofactors -- Biosynthesis, Insertion and Function of haem-iron Prosthetic Groups -- Computational Framework for Integration of Lipidomics Data into Metabolic Pathways -- Degradation of Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) -- Degradation of Rubber/Polyisoprene -- Diversity and Common Principles in Enzymatic Activation of Hydrocarbons: An Introduction -- Energetic and Other Quantitative Aspects of Microbial Hydrocarbon Utilization: An Introduction: -- Enzymes for Aerobic Degradation of Alkanes in Bacteria -- Enzymes for Aerobic Degradation of Alkanes in Yeasts -- Evolution of New Catabolic Functions Through Gene Assembly by Mobile Genetic Elements -- Experimental Evolution of Novel Regulatory Activities in Response to Hydrocarbons and Related Chemicals -- Functional Gene Diversity, Biogeography, Dynamics -- Genetic Features and Regulation of n-Alkane Metabolism in Bacteria -- Genetic Features and Regulation of n-Alkane Metabolism in Yeasts -- Genetic Features of Methylotrophs -- Genetics and Functional Genomics of Aerobic Degradation of Hydrocarbons: An Introduction -- Genetics and Molecular Features of Bacterial Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) Transformations -- Genetics of Biphenyl Biodegradation and Co-Metabolism of PCBs -- Genetics of Rubber/Polyisoprene Degradation -- Genetics and Ecology of Isoprene Degradation -- Genomic View of Mycobacterial High Molecular Weight Polycyclic Aromatic Hydrocarbon Degradation -- Global Aerobic Degradation of Hydrocarbons in Aquatic Systems -- Global Aerobic Degradation of Hydrocarbons in Terrestrial Systems -- Global Consequences of the Microbial Production and Consumption of Inorganic and Organic Sulfur Compounds -- Key Features of Aerobic Hydrocarbon Biodegrader Genomes -- Lipid Degradation - Lipid Cycles -- Lipolytic Enzymes from Bacteria -- Lipolytic Enzymes from Yeasts -- Metabolism of Steroids -- Oxidative Inactivation of Ring-Cleavage Extradiol Dioxygenases: Mechanism and Ferredoxin-Mediated Reactivation -- Pathways for the Degradation of Fatty Acids in Bacteria -- Phylogenomics of Aerobic Bacterial Degradation of Aromatics -- Physiology and Biochemistry of the Aerobic Methane Oxidizing Bacteria -- Potential for Microbial Interventions to Reduce Global Warming -- Rational Construction of Bacterial Strains with New/Improved Catabolic Capabilities for the Efficient Breakdown of Environmental Pollutants -- Regulation of the Degradation of Fatty Acids in Bacteria -- Regulation of the Degradation of Fatty Acids in Yeast -- Stereochemistry of Hydrocarbon-transforming Enzyme Reactions -- Structure-Function Relationships and Engineering of Haloalkane Dehalogenases -- Transcriptional Control of the TOL Plasmid Pathways
    Abstract: This book describes the biochemistry and genetics of aerobic degradation of hydrocarbons and lipids by bacteria and yeasts, as well as the functional genomics of the best-known microorganisms involved in these processes. It forms part of the Handbook of Hydrocarbon and Lipid Microbiology Series, a definitive resource of current knowledge on the diverse aspects of the interactions of microbes with hydrocarbons and lipids. Chapters are mainly focused on the new discoveries of recent years. It is aimed to scientists and others interested in different aspects of the microbiology of hydrocarbons
    Pages: 149 illus., 43 illus. in color. eReference. : online resource.
    ISBN: 9783319504186
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  • 10
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    Singapore : Springer
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Molecular Basis for Oxidative Stress Induced by Environmental Toxicants in Nematodes -- Molecular Basis for Reduced Lifespan Induced by Environmental Toxicants or Stresses -- Roles of Oxidative Stress Related Molecular Signals in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of MAPK Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Insulin and the Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Development Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Cell Death and DNA Damage Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Metabolism Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Protective Response Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of G-Protein Coupled Receptors and Ion Channels and the Downstream Cytoplasmic Signals in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Discussion on Specificity of Molecular Signals in Response to Certain Environmental Toxicants or Stresses -- Epigenetic Regulation of Toxicity of Environmental Toxicants or Stresses -- Strategies to Screen and to Identify New Genetic Loci Involved in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Molecular Basis for Adaptive Response to Environmental Toxicants or Stresses -- Molecular Basis for Transgenerational Toxicity Induction of Environmental Toxicants or Stresses
    Abstract: This book will focus on the molecular basis of oxidative stress induced by toxicants or stresses and various molecular signalling pathways in regulating the toxicity of toxicants or stresses in Caenorhabditis elegans. It will also cover the discussion on the aspects of response signals, G-protein coupled receptors and ion channels, specific molecular signals, and epigenetic signals involved in the regulation of toxicity from toxicants or stresses. The molecular basis for adaptive response for transgenerational toxicity of environmental toxicants or stresses will be further discussed. Nematode Caenorhabditis elegans is a classic model animal with well-described genetic and developmental backgrounds based on the study of life science, and has been further successfully and widely used in both toxicity assessment and toxicological study of various environmental toxicants or stresses. Based on related available data, this book aims at providing a systematic understanding of the knowledge system of molecular toxicology in C. elegans
    Pages: XVIII, 447 p. 285 illus., 211 illus. in color. : online resource.
    ISBN: 9789811336331
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  • 11
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Normal Growth in PKU Patients Under Low-Protein Diet in a Single-Center Cross-Sectional Study -- Serial Magnetic Resonance Imaging and 1H-Magnetic Resonance Spectroscopy in GABA Transaminase Deficiency: A Case Report.-Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment -- Cognitive and Behavioural Outcomes of Paediatric Liver Transplantation for Ornithine Transcarbamylase Deficiency -- Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation -- Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice -- Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period -- Severe Leukoencephalopathy with Clinical Recovery Caused by Recessive BOLA3 Mutations -- Neonatal Onset Interstitial Lung Disease as a Primary Presenting Manifestation of Mucopolysaccharidosis Type I -- A Middle Eastern Founder Mutation Expands the Genotypic and Phenotypic Spectrum of Mitochondrial MICU1 Deficiency: A Report of 13 Patients -- Disruption of the Responsible Gene in a Phosphoglucomutase 1 Deficiency Patient by Homozygous Chromosomal Inversion -- Evaluation of Disease Lesions in the Developing Canine MPS IIIA Brain -- Extrapolation of Variant Phase in Mitochondrial Short-Chain Enoyl-CoA Hydratase (ECHS1) Deficiency -- RFT1-CDG: Absence of Epilepsy and Deafness in Two Patients with Novel Pathogenic Variants -- Short-Term Administration of Mycophenolate Is Well-Tolerated in CLN3 Disease (Juvenile Neuronal Ceroid Lipofuscinosis)
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 35 illus., 20 illus. in color. : online resource.
    ISBN: 9783662586143
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  • 12
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 13
    Keywords: Immunology ; Immunology ; Springer eBooks
    Description / Table of Contents: Isolation and Characterisation of Human Adipose-derived Stem Cells -- Isolation of Murine Hematopoietic Stem Cells -- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells -- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting -- Identification and isolation of Regulatory B cells in Mouse and Human -- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis -- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells -- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade -- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells -- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays -- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments -- Immune tolerance and rejection in organ transplantation -- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation -- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood -- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental -- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
    Abstract: This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 240 p. 43 illus., 36 illus. in color. : online resource.
    ISBN: 9781493989386
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  • 14
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Making the Case for Functional Proteomics -- Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors -- Two-dimensional 16-BAC/SDS polyacrylamide gel electrophoresis of mitochondrial membrane proteins -- Systematic Glycolytic Enzyme Activity Analysis from Human Serum with PEP Technology -- A protein decomplexation strategy in snake venom proteomics -- Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level -- A systematic analysis workflow for high-density customized protein microarrays in biomarker screening -- Metaproteomics study of the gut microbiome -- Double-One Dimensional Electrophoresis (D1-DE) Adapted for Immunoproteomics -- BioID: A proximity dependent labeling approach in proteomics study -- Functional application of snake venom proteomics in in vivo antivenom assessment -- Proteomic detection of carbohydrate-active enzymes (CAZymes) in microbial secretomes -- An Overview of Mass Spectrometry-based Methods for Functional Proteomics -- Functional proteomic analysis to characterize signaling crosstalks -- Identification of unexpected protein modifications by mass spectrometry based proteomics -- Label-free LC-MS/MS Strategy for Comprehensive Proteomic Profiling of Human Islets Collected using Laser Capture Microdissection from Frozen Pancreata -- Mass Spectrometry based targeted proteomics for cell and tissue analysis -- Metabolomic investigation of Staphylococcus aureus antibiotic susceptibility by liquid chromatography coupled to high resolution mass spectrometry -- Nuts and bolts of protein quantification by on-line trypsin digestion coupled LC-MS/MS analysis -- Proteases: Pivot Points in Functional Proteomics -- The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics -- Efficient Extraction and Digestion of Gluten Proteins -- Glycosylation profiling of tumor marker in plasma using bead-based immunoassay -- Protein-specific analysis of invertebrate glycoproteins -- The use of proteomics studies in identifying moonlighting proteins -- Two-dimensional biochemical purification for global proteomic analysis of macromolecular protein complexes -- A data analysis protocol for quantitative data-independent acquisition proteomics
    Abstract: This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed
    Pages: XII, 477 p. 89 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988143
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  • 15
    Keywords: Biotechnology ; Human Genetics ; Biotechnology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Microinjection and Micromanipulation: A Historical Perspective -- Production of Transgenic Mice by Pronuclear Microinjection -- Transgene Recombineering in Bacterial Artificial Chromosomes -- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice -- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method -- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer -- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection -- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice -- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique -- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation -- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides -- Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection -- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications -- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm -- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line -- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology -- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos -- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells -- Generation of Rabbit Models by Gene Editing Nucleases -- Production of Genetically Engineered Porcine Embryos by Handmade Cloning -- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production -- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer -- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting -- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes -- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing -- Microinjection in Zebrafish for Genome Editing and Functional Studies -- Microinjection of Marine Fish Eggs -- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method -- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus -- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function
    Abstract: This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations
    Pages: XIV, 540 p. 134 illus., 96 illus. in color. : online resource.
    ISBN: 9781493988310
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  • 16
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Protocol to Compare Methods for Untargeted Metabolomics -- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry -- Measuring the Nutrient Metabolism of Adherent Cells in Culture -- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells -- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice -- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro -- Measuring Rates of ATP Synthesis -- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay -- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers -- Determining Compartment Specific Metabolic Fluxes -- Determining the Impact of Metabolic Nutrients on Autophagy -- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism -- Determining Macrophage Polarization upon Metabolic Perturbation -- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells -- Development of Patient-derived Tumor Xenograft Models -- Imaging Glioma Progression by Intravital Microscopy -- Lipectomizing Mice for Applications in Metabolism -- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants -- Determining the Intracellular Organization of Organelles -- The Fundamentals of Constructing and Interpreting Heat Maps
    Abstract: This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level
    Pages: XII, 294 p. 67 illus., 53 illus. in color. : online resource.
    ISBN: 9781493987696
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  • 17
    Keywords: Oncology ; Neurosciences ; Cancer Research ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Introduction to Brain Tumor Stem Cells -- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells -- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines -- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors -- Detecting Stem Cell Marker Expression Using the NanoString nCounter System -- Flow Cytometric Analysis of Brain Tumor Stem Cells -- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells -- Differentiation of Brain Tumor Initiating Cells -- The Study of Brain Tumor Stem Cell Migration -- The Study of Brain Tumor Stem Cell Invasion -- Cell Cycle Dynamics in Glioma Cancer Stem Cells -- Embryonic Stem Cell Models of Human Brain Tumors -- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory -- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF -- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells -- In Vitro Assays for Screening Small Molecules -- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma -- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci -- In Vivo Murine Models of Brain Metastasis -- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain
    Abstract: This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer
    Pages: XII, 254 p. 40 illus., 34 illus. in color. : online resource.
    ISBN: 9781493988051
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  • 18
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Overview of BCL-2 Family Proteins and Therapeutic Potentials -- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins -- Application of Mito-Priming to Generate BCL-2 Addicted Cells -- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies -- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP) -- Investigating BCL-2 Family Protein Interactions in Yeast -- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family -- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP -- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family -- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol -- Quantification of BCL-2 Family Members by Flow Cytometry -- Methods to Probe Calcium Regulation by BCL-2 Family Members -- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK -- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage -- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs -- Cryo-Electron Microscopy to Study Bax Pores and MOMP -- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores -- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET -- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy -- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX)
    Abstract: This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, BCL-2 Family Proteins: Methods and Protocols is a valuable resource to inspire and encourage novice and established scientists to further their research and make new discoveries in this exciting field
    Pages: XI, 360 p. 96 illus., 66 illus. in color. : online resource.
    ISBN: 9781493988617
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  • 19
    Keywords: Biotechnology ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Gene Regulatory Network Inference: An Introductory Survey -- Statistical Network Inference for Time-Varying Molecular Data with Dynamic Bayesian Networks -- Overview and Evaluation of Recent Methods for Statistical Inference of Gene Regulatory Networks from Time Series Data -- Whole-Transcriptome Causal Network Inference with Genomic and Transcriptomic Data -- Causal Queries from Observational Data in Biological Systems via Bayesian Networks: An Empirical Study in Small Networks -- A Multiattribute Gaussian Graphical Model for Inferring Multiscale Regulatory Networks: An Application in Breast Cancer -- Integrative Approaches for Inference of Genome-Scale Gene Regulatory Networks -- Unsupervised Gene Network Inference with Decision Trees and Random Forests -- Tree-Based Learning of Regulatory Network Topologies and Dynamics with Jump3 -- Network Inference from Single-Cell Transcriptomic Data -- Inferring Gene Regulatory Networks from Multiple Datasets -- Unsupervised GRN Ensemble -- Learning Differential Module Networks across Multiple Experimental Conditions -- Stability in GRN Inference -- Gene Regulatory Networks: A Primer in Biological Processes and Statistical Modelling -- Scalable Inference of Ordinary Differential Equation Models of Biochemical Processes
    Abstract: This volume explores recent techniques for the computational inference of gene regulatory networks (GRNs). The chapters in this book cover topics such as methods to infer GRNs from time-varying data; the extraction of causal information from biological data; GRN inference from multiple heterogeneous data sets; non-parametric and hybrid statistical methods; the joint inference of differential networks; and mechanistic models of gene regulation dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, descriptions of recently developed methods for GRN inference, applications of these methods on real and/ or simulated biological data, and step-by-step tutorials on the usage of associated software tools. Cutting-edge and thorough, Gene Regulatory Networks: Methods and Protocols is an essential tool for evaluating the current research needed to further address the common challenges faced by specialists in this field
    Pages: XI, 430 p. 114 illus., 71 illus. in color. : online resource.
    ISBN: 9781493988822
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  • 20
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    New York, NY : Springer
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: In Vitro Methods for Assessing Nanoparticle Toxicity -- Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure -- Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment -- Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing -- Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles -- In Vitro Approaches for Assessing the Genotoxicity of Nanomaterials -- Measurement of Nanoparticle Induced Mitochondrial Membrane Potential Alterations -- Application of Gelatin Zymography in Nanotoxicity Research -- Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies -- Analysis of Nanomaterial Toxicity by Western Blot -- Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR -- DNA Methylation Analysis -- Evaluation of Nrf2 with Exposure to Nanoparticles -- Application of Electron Microscopes in Nanotoxicity Assessment -- Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry -- Methods to Quantify Nanomaterial Association with, and Distribution Across, the Blood-Brain Barrier In Vivo -- Evaluation of Genotoxicity of Nanoparticles in Mouse Models -- Evaluation of Pulmonary Toxicity of Nanoparticles by Bronchoalveolar Lavage -- Pulmonary Function Testing in Animals -- Nanotoxicity Assessment Using Embryonic Zebrafish -- Application of Daphnia magna for Nanoecotoxicity Study -- The Overview of Methods of Nanoparticle Exposure Assessment
    Abstract: This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field
    Pages: XII, 371 p. 68 illus., 52 illus. in color. : online resource.
    ISBN: 9781493989164
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  • 21
    Keywords: Stem Cells ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells -- Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver -- Identification and Isolation of Clonogenic Cholangiocyte in Mouse -- Isolation and Expansion of Rat Hepatocytic Progenitor Cells -- Genetic Lineage Tracing of Biliary Epithelial Cells -- Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination -- Analysis for the Heterogeneity of Liver Progenitor Cells -- Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration -- Conversion of Fibroblasts to Hepatocytes In Vitro -- Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo -- Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells -- Induction of Functional Hepatocytes from Human iPSCs -- Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells -- Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture -- Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device -- Generation of Hepatic Organoids with Biliary Structures -- Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration -- Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells -- A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells -- Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes -- Mouse Model for Cholangiocarcinoma from Peribiliary Glands
    Abstract: This volume looks at the liveŕ€™s epithelial cellś€”hepatocytes and cholangiocytes€”ánd their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology
    Pages: XI, 248 p. 70 illus., 59 illus. in color. : online resource.
    ISBN: 9781493989614
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  • 22
    Keywords: Human Genetics ; Oncology ; Human Genetics ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Cell Free DNA: Applications in Different Diseases -- Cell Free DNA: An Overview of Sample Types and Isolation Procedures -- Circulating Cell Free DNA and Cancer Therapy Monitoring: Methods and Potential -- Cell Free DNA in the Liquid Biopsy Context: Role and Differences between ctDNA and CTC Marker in Cancer Management -- Cell Free DNA Integrity: Applications -- Copy Number Variation Analysis on Cell Free Serum DNA -- Studying Copy Number Variations in Cell Free DNA: The Example of AR in Prostate Cancer -- dPCR Mutational Analyses in Cell-free DNA: A Comparison With Tissues -- From cfDNA to Sequencing: Workflows and Potentials -- Epigenetic Characterization of Cell Free DNA -- Quantitative Methylation-specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell Free DNA -- Cell Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges -- Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma -- Circulating Cell-Free DNA in Physical Activities -- Urinary Cell Free DNA: Potential and Applications -- Urinary Cell Free DNA: Isolation, Quantification, Quality Assessment
    Abstract: This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: X, 224 p. 29 illus., 27 illus. in color. : online resource.
    ISBN: 9781493989737
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  • 23
    Keywords: Neurosciences ; Biochemistry ; Neurosciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Co-Immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue -- Co-Immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain Tissue -- Use of Co-Immunoprecipitation to Study Conformation-Specific protein Interactions of Oligomeric α-Synuclein Aggregates -- Using Co-Immunoprecipitation and Shotgun Mass Spectrometry for protein-Protein Interaction Identification in Cultured Human Oligodencrocytes -- Co-Immunoprecipition Analysis of GPCR Complexes in the Central Nerve System -- The Histoblot Technique: A Reliable Approach to Analyse Expression Profile of proteins and to Predict their Molecular Association -- Co-Immunoprecipitation Assay to Investigate the Interaction Strength between Synaptic Protein using COS-7 Cells -- Guanosine-5’-O-(3-[35S]thio)triphosphate ([35S]GTPS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-G Antibody in Mammalian Brain Membranes -- Co-Immunoprecipitation as a Useful Tool for Detection of G protein-Coupled Receptors Oligomers -- Isolation and Detection of G Protein–Coupled receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation using a Combined Brain Subcellular Fractionation/Co-Immunoprecipitation (co-IP) Procedures -- Co-Immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments -- Co-Immunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita during Aestivation -- Co-Immunoprecipitation (co-IP) of G Protein-Coupled Receptors (GPCR)-Receptor Tyrosine Kinases (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain
    Abstract: This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field
    Pages: XIII, 166 p. 28 illus., 19 illus. in color. : online resource.
    ISBN: 9781493989850
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  • 24
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Introduction to FOXO Biology -- Characterization of microRNAs Regulating FOXO Expression -- Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter -- Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover -- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity -- Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes -- Characterization of FOXO Acetylation -- Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays -- Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes -- Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters -- RNAi Mediated Silencing of FoxO Factors -- Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO -- High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO -- Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus -- Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology -- Genome-wide Analysis for Identifying FOXO Protein Binding Sites -- Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors -- Following Transcriptome to Uncover FOXO Biological Functions -- Hydra as Model to Determine the Role of FoxO in Longevity -- Genetic Ablation of FOXO in Mice to Investigate its Physiological Role -- Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity -- Analysis of FOXO3a Gene Polymorphism Associated with Asthma
    Abstract: This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 270 p. 54 illus., 33 illus. in color. : online resource.
    ISBN: 9781493989003
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  • 25
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Arthropod Genome Sequencing and Assembly Strategies -- Genome Size Estimation and Quantitative Cytogenetics in Insects -- Isolation of HMW DNA from Insects Chapter -- Long range sequencing and assembly QC -- Integrated modeling of structural genes using MCuNovo -- Using BUSCO to Assess Insect Genomic Resources -- The GFF3toolkit—QC and Merge Pipeline for Genome Annotation -- Using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) to identify functional regulatory DNA in insect genomes -- Using RAMPAGE to identify and annotate promoters in insect genomes -- CRM Beyond Model Insects -- Whole-genome bisulfite sequencing for the methylation analysis of insect genomes -- Bioinformatic analysis of methylation patterns using bisulfite sequencing data -- Physical Genome Mapping Using Fluorescence in Situ Hybridization with Mosquito Chromosomes -- Target enriched RAD sequencing (TEEseq): A new high-throughput sequencing approach applied to the comprehensive characterization of endosymbionts
    Abstract: This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field
    Pages: XI, 237 p. 34 illus., 23 illus. in color. : online resource.
    ISBN: 9781493987757
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  • 26
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Microbial Metabolomics: A General Overview -- Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications -- Metabolomics: A Microbial Physiology and Metabolism Perspective -- Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry -- Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis -- Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis -- High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics -- Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry -- Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis -- Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS) -- Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli -- Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling -- Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis -- Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry -- A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism -- Cluster Analysis of Untargeted Metabolomic Experiments -- Machine Learning in Untargeted Metabolomics Experiments -- Dynamic 13C Labeling of Fast Turnover Metabolites for Analysis of Metabolic Fluxes and Metabolite Channeling -- Genome-Scale 13C Fluxomics Modeling for Metabolic Engineering of Saccharomyces cerevisiae
    Abstract: This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher
    Pages: X, 351 p. 83 illus., 50 illus. in color. : online resource.
    ISBN: 9781493987573
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  • 27
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 28
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Physiological Characterization of Phototropism in Arabidopsis Seedlings -- Assessing Negative and Positive Phototropism in Lianas -- Determination of Phototropism and Polarotropism in Fern Protonemal Cells -- Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli -- An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens -- Observation of Phototropic Responses in the Liverwort Marchantia polymorpha -- Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus -- Quantitative Analysis of Tip Growth, Phototropic Responses and Other Blue Light-Dependent Photoresponses of Vaucheria -- A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezonensis -- Determination of Phototropism by UV-B Radiation -- New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport -- Determination of Auxin Flow during Phototropic Responses Using Fluorescent Auxin Analogs -- Immunolocalization of IAA Using an Anti-IAA-C Antibody Raised against Carboxyl-Linked IAA -- Measurements of Photoreaction and Kinase Activity of Phototropin, a Photoreceptor Protein for Tropic Response in Plants; Involvement of Kinase Activity in the Photosensitivity of Tropic Response -- Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema -- Grafting as a Tool for Study of Phototropism in Phycomyces -- Preparation of a Spaceflight Experiment to Study Tropisms in Arabidopsis Seedlings on the International Space Station -- How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening -- Quantitative Measurements of Curvature along the Growth Axis in Tropic Responses Using Free Software Environments
    Abstract: This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin. In addition, the use of grafting, spaceflight experiment and image analysis in tropism study is provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phototropism: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: X, 240 p. 89 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990153
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  • 29
    Keywords: Biochemistry ; Medical laboratories ; Protein Science ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Using Data Mining to Explore Calmodulin Bibliography -- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator -- High Resolution Calcium Imaging Method for Local Calcium Signaling -- Measurement of Contractility and Calcium Release in Cardiac Spheroids -- Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis -- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio) -- Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements -- Designing Calcium Binding Proteins for Molecular MR Imaging -- Coordination to Divalent Cations by Calcium Binding Proteins -- Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo -- Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets -- Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes -- Expression and Purification of Calmodulin for NMR and Other Biophysical Applications -- The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells -- Calpain Purification through Calpastatin and Calcium: Strategy and Procedures -- Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins -- Expression and Characterization of MICU2, a Ca2+ Sensor Protein -- S100 Proteins in the Innate Immune Response to Pathogens -- Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors -- Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy -- Isolation and Characterization of S100 Protein-Protein Complexes -- The Multifaceted S100A4 Protein in Cancer and Inflammation -- Interaction of S100A6 with Target Proteins In Vitro and in Living Cells -- Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7) -- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin -- Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography -- Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex -- Analysis of S100A11 in DNA Damage Repair -- Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography -- Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure -- High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins -- Calcium-Induced Protein Folding in Calumenin and Calmodulin -- Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing -- Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies -- Tryptophan Scanning Mutagenesis of EF-Hand Motifs -- Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling -- Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level -- Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation -- Characterization of Calcium-Binding Proteins from Parasitic Worms -- High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome -- Targeted Mass Spectrometry of S100 Proteins -- Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury -- Serum S100B Levels in Melanoma -- The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine -- S100A7 in Psoriasis: Immunodetection and Activation by CRISPR -- S100A8/A9 in Myocardial Infarction -- Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults -- S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions
    Abstract: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry
    Pages: XIX, 779 p. 175 illus., 117 illus. in color. : online resource.
    ISBN: 9781493990306
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  • 30
    Keywords: Plant breeding ; Agriculture ; Plant Breeding/Biotechnology ; Agriculture ; Springer eBooks
    Description / Table of Contents: Hybrid Sorghum Product Development and Production -- Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum -- Field Studies to Develop Weed Management Programs for Grain Sorghum -- Discovery of Sorghum Haploid Induction System -- Development of a Pedigreed Sorghum Mutant Library -- Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion -- Assaying Sorghum Nutritional Quality -- Tannin Analysis in Sorghum Grains -- Sorghum Phytochemicals and their Potential Impact on Human Health -- Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography -- Gene Editing in Sorghum through Agrobacterium -- Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment -- Novel Ternary Vectors for Efficient Sorghum Transformation -- Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa -- Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions -- Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case -- Background on Grain Sorghum Usage -- Assaying Sorghum for Fuel Production -- The Role of Sorghum in Renewables and Biofuels
    Abstract: This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum
    Pages: X, 281 p. 84 illus., 61 illus. in color. : online resource.
    ISBN: 9781493990399
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  • 31
    Keywords: Medicine ; Oncology ; Molecular Medicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Amplicon-based Targeted Next Generation Sequencing of Formalin Fixed Paraffin Embedded Tissue -- Library preparation using FFPE-derived Tumor DNA for High Throughput Hybridization-based Targeted or Exome Sequencing -- Bioinformatics Basics for High Throughput Hybridization-based Targeted DNA Sequencing From FFPE-Derived Tumor Specimens: From Reads to Variants -- Annotation of Variant Data from High Throughput DNA Sequencing from Tumor Specimens: Filtering Strategies to Identify Driver Mutations -- Biological Interpretation of Complex Genomic Data -- Clinical Validation of Targeted Solid Tumor Profiling -- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors -- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation -- Overview of Fusion Detection Strategies Using Next Generation Sequencing -- Clinical Application of Fusion Gene Detection Using Next-generation Sequencing and the NanoString Technology -- Pipeline for Integrated Microarray Expression Normalization Toolkit (PIMENTo) for Tumor Microarray Profiling Experiment -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: Overview of Library Preparation Methods -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: From Raw Data to Systems Level Analyses -- Methylation Analysis using Microarrays: Analysis and Interpretation -- High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) -- A Pipeline for ctDNA Detection Following Primary Tumor Profiling Using A Cancer-related Gene Sequencing Panel -- Single-cell Analysis of Circulating Tumor Cells
    Abstract: This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field
    Pages: XI, 268 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493990047
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  • 32
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 33
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinsoń€™s Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- ℗Ư℗ƯMonitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the €œáutophagic flux́€ in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations
    Abstract: This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XVII, 741 p. 137 illus., 99 illus. in color. : online resource.
    ISBN: 9781493988730
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  • 34
    Keywords: Bioinformatics ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 1. PREDECT protocols for complex 2D/3D cultures -- 2. Phenotypic Screening using Mouse and Human Stem Cell-based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses -- 3. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer -- 4. Target Engagement of Small Molecules – Thermal Profiling Approaches on Different Levels -- 5. Visualizing Bio-active Small Molecules by Alkyne-tagging and Slit-scanning Raman Microscopy -- 6. The Cell Painting Assay as a screening tool for the discovery of bioactivities in new chemical matter -- 7. Proteomic Profiling for Target Identification of Biologically Active Small Molecules using 2-D DIGE -- 8. Examining Cellular Responses to Kinase Drug Inhibition through Phosphoproteome Mapping of Substrates -- 9. CRISPR/Cas9-based Chemogenomic Profiling in Mammalian Cells -- 10. Exome Sequencing of Drug-resistant Clones for Target Identification -- 11. The use of large-scale chemically-induced transcriptome data acquired from LINCS to study small molecules -- 12. Integrated Analysis of Drug Sensitivity and Selectivity to Predict Synergistic Drug Combinations and Target Co-addictions in Cancer -- 13. Predicting Drug Interactions from Chemogenomics using INDIGO -- 14. Computational Analyses Connect Small-Molecule Sensitivity to Cellular Features Using Large Panels of Cancer Cell Lines -- 15. Web-based tools for Polypharmacology Prediction -- 16. In silico Target Prediction for Small Molecules
    Abstract: This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level
    Pages: XI, 315 p. 65 illus., 43 illus. in color. : online resource.
    ISBN: 9781493988914
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  • 35
    Keywords: Pharmaceutical technology ; Biochemistry ; Bioinformatics ; Pharmaceutical Sciences/Technology ; Medicinal Chemistry ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: Methods for Discovering and Targeting Druggable Protein-Protein Interfaces and Their Application to Repurposing -- Performing an In Silico Repurposing of Existing Drugs by Combining Virtual Screening and Molecular Dynamics Simulation -- Repurposing Drugs Based on Evolutionary Relationships between Targets of Approved Drugs and Proteins of Interest -- Drug Repositioning by Mining Adverse Event Data in ClinicalTrials.gov -- Transcriptomic Data Mining and Repurposing for Computational Drug Discovery -- Network-Based Drug-Repositioning: Approaches, Resources, and Research Directions -- A Computational Bipartite-Graph-Based Drug Repurposing Method -- Implementation of a Pipeline Using Disease-Disease Associations for Computational Drug Repurposing -- An Application of Computational Drug Repurposing Based on Transcriptomic Signatures -- Drug-Induced Expression-Based Computational Repurposing of Small Molecules Affecting Transcription Factor Activity -- A Drug Repurposing Method Based on Drug-Drug Interaction Networks and Using Energy Model Layouts -- Integrating Biological Networks for Drug Target Prediction and Prioritization -- Using Drug Expression Profiles and Machine Learning Approach for Drug Repurposing -- Computational Prediction of Drug-Target Interactions via Ensemble Learning -- A Machine-Learning-Based Drug Repurposing Approach Using Baseline Regularization -- Machine Learning Approach for Predicting New Uses of Existing Drugs and Evaluation of Their Reliabilities -- A Drug-Target Network-Based Supervised Machine Learning Repurposing Method Allowing the Use of Multiple Heterogeneous Information Sources -- Heter-LP: A Heterogeneous Label Propagation Method for Drug Repositioning -- Tripartite Network-Based Repurposing Method Using Deep Learning to Compute Similarities for Drug-Target Prediction
    Abstract: This detailed book explores techniques commonly used for research into drug repurposing, a well-known strategy to find alternative indications for drugs which have already undergone toxicology and pharma-kinetic studies but have failed later stages during the development, via computational methods. Thereby, it addresses the intense challenges of identifying the appropriate type of algorithm and relevant technical information for computational repurposing. Written for the highly successful Methods in Molecular Biology series, the authors of each chapter use their experience in the field to describe the implementation and successful use of a specific repurposing method thus providing lab-ready instruction. Authoritative and practical, Computational Methods for Drug Repurposing serves as an ideal guide to researchers interested in this vital area of drug development
    Pages: XI, 332 p. 103 illus., 82 illus. in color. : online resource.
    ISBN: 9781493989553
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  • 36
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer -- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood -- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells -- Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo -- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors -- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance -- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity -- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro -- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency -- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity -- Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo -- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments -- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells -- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry -- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways -- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation -- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion -- Quantitative Identification of Senescent Cells in Cancer -- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance -- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment -- Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models -- A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers -- Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy -- Generation of CAR-T Cells for Cancer Immunotherapy
    Abstract: This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field
    Pages: XIII, 363 p. 76 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988853
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  • 37
    Keywords: Electrophoresis ; Biotechnology ; Electrophoresis ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Fabrication of Glass Microfluidic Devices -- Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices -- Sample Injection Techniques -- Sample Preconcentration Protocols in Microfluidic Electrophoresis -- Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection -- Micellar Electrokinetic Chromatography -- Microchip Isotachophoresis: Analysis of Pharmaceuticals -- Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination -- Nanochannel Gradient Separations -- Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications -- Band Broadening Theories in Capillary Electrophoresis -- Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation -- Microchip Electrophoresis Tools for the Analysis of Small Molecules -- Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out -- Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips -- Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow
    Abstract: This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals
    Pages: X, 255 p. 93 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989645
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  • 38
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Power of Drosophila Genetics: The Discovery of the Hippo Pathway -- Drosophila Genetics: The Power of Genetic Mosaic Approaches -- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues -- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs -- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence -- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions -- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells -- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells -- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors -- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells -- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells -- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays -- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids -- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview -- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels -- Hippo Pathway Regulation by Tyrosine Kinases -- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway -- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro -- GST Pull-Down Assay to Measure Complex Formations -- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag -- Quantifying the Kinase Activities of MST1/2 -- Measuring the Kinase Activities of the LATS/NDR Protein Kinases -- MST1/2 Kinase Assays Using Recombinant Proteins -- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development -- The Hippo Signaling Pathway in Regenerative Medicine
    Abstract: This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings
    Pages: XIV, 374 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493989102
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  • 39
    Keywords: Biochemistry ; Cell Membranes ; Biochemistry, general ; Membrane Biology ; Biological and Medical Physics, Biophysics ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- 1. Understanding Membranes and Vesicles: A Personal Recollection of the Last Two Decades -- 2. Advanced Concepts and Perspectives of Membrane Physics -- Part 2: Membranes by the Numbers -- 3. Membranes by the Numbers -- Part 3: Spatial Heterogeneities in Biomembranes -- 4. LIPID RAFTS – A PERSONAL ACCOUNT -- 5. Theories of Equilibrium In homogeneous Fluids -- 6. Critical lipidomics: The consequences of lipid miscibility in biological membranes -- 7. Lateral diffusion in heterogeneous cell membranes -- 8. Mechanical factors affecting the mobility of membrane proteins -- 9. Membrane domains under cellular recycling -- 10. Protein Pattern Formation -- Part 4: Biomembrane Mechanics and Consequences for their Functions -- 11. Biomembrane mechanical properties direct diverse cell functions -- 12. Spontaneous and intrinsic curvature of lipid membranes: back to the origins -- 13. Membrane-mediated interactions -- 14. Simulating protein-mediated membrane remodeling at multiple scales -- 15. Mechanosensitivity of membrane budding and tracking Lionel Foret -- 16. Common energetic and mechanical features of membrane fusion and fission machineries -- 17. Interaction of particles and pathogens with biological membranes -- 18. Adhesion of biological membranes -- 19. Spatial and mechanical aspects of signal transduction in the cell membrane -- 20. Protein-Induced morphological deformations of biomembranes -- 21. Fluctuations in active membranes
    Abstract: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike
    Pages: X, 623 p. 167 illus., 144 illus. in color. : online resource.
    ISBN: 9783030006303
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  • 40
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    New York : Springer
    Associated volumes
    Call number: QZ202.6:30(2)
    Keywords: AIDS malignancies ; Viral carcinogenesis ; Oncogenic Viruses ; Tumor Virus Infections ; Neoplasms / etiology ; Acquired Immunodeficiency Syndrome
    Pages: viii, 250 p. : ill. (some col.)
    Edition: 2nd ed.
    ISBN: 9783030035013
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  • 41
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    New York, NY : Springer
    Keywords: Immunology ; Medical virology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus -- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV -- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR) -- Establishment of Replication-competent HCV Strain with Minimum Modifications -- Full-length Open Reading Frame Amplification of Hepatitis C Virus -- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing -- A Cell Culture Model for Persistent HCV Infection -- Expression Cloning of Host Factors Required for the HCV Replication Cycle -- Application of Deep Mutational Scanning in Hepatitis C Virus -- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals -- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay -- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells -- Functional Characterization of Apolipoproteins in the HCV Life Cycle -- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes -- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins -- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis -- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells -- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact -- Complement Regulation and Immune Evasion by Hepatitis C Virus -- Direct ex vivo Functional Analysis of HCV-specific T Cells -- ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry -- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display -- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA -- In vitro Neutralization Assay using Cultured Hepatitis C Virus -- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp) -- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice -- Mice with Chimeric Human Livers and Their Applications -- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies
    Abstract: This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIX, 508 p. 75 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989768
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  • 42
    Keywords: Microbiology ; Food science ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products -- Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens -- The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens -- Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection -- Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices -- Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria -- EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes -- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions -- Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation -- Isolation of Bacterial RNA from Foods Inoculated with Pathogens -- The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress -- Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics -- Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells -- High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens -- Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae -- Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection -- Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback -- The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform -- Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics -- Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments -- Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars
    Abstract: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens
    Pages: XI, 278 p. 53 illus., 43 illus. in color. : online resource.
    ISBN: 9781493990009
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  • 43
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 44
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: AcetylCoA Synthesis and Regulation -- AcetylCoA Synthesis and Role in Bacteria -- Autophagy in Stationary Phase of Growth -- Bacterial Sphingolipids -- Cell-contact-dependent Outer Membrane Exchange in Myxobacteria -- Contributions of Membrane Lipids to Bacterial Cell Homeostasis Upon Osmotic Challenge -- Dynamic Membrane Structure: Function Analysis by Means of Chemical Probes -- Evolution of Hydrophobic Storage Polymers -- Evolution of Lipids -- Evolution of Membranes -- Fatty Acid Synthesis and Regulation -- Fatty Acids: Introduction -- Formation of Bacterial Glycerol-based Membrane Lipids: Pathways, Enzymes, Reactions -- Formation of Fatty Acids -- Formation of Hopanoids -- Formation of Isoprenoids -- Formation of Lipochitin Oligosaccharide Signaling Molecules -- Functional Roles of Individual Membrane Lipids in Bacteria, Archaea and Eukaryotic Microbes -- Functional Roles of Non-membrane Lipids in Bacterial Signaling -- Hopanoids and Membrane Integrity and pH Homeostasis -- Lipid A -- Lipid Chaperones -- Lipid Chaperones and Regulation -- Lipid Intermediates in Bacterial Peptidoglycan Biosynthesis -- Lipid Metabolism in Microalgae -- Lipid Rafts -- Lipid-protein Interactions -- Lipid-protein Interactions Determining Membrane Fluidity in Prokaryotes and Eukaryotes -- Lipids as Receptors -- Lipoteichoic Acid Synthesis and Function in Gram-positive Bacteria -- Membrane Disrupting Proteins -- Membrane Formation and Regulation -- Membrane Homeostasis Upon Nutrient (C, N, P) Limitation -- Membrane Homeostasis upon pH Challenge -- Membrane Lipid Biogenesis -- Membrane Structure:Function Analysis by Means of Chemical Probes -- Membrane Structure:Function Analysis Through Reconstitution In Vitro -- Membrane Vesicles, Nanopods and Nanotubes of Archaea -- Metabolism and Regulation of Glycerolipids in Yeast -- Metabolism and Roles of Sphingolipids in Yeast Saccharamyces cerevisiae -- Modeling Lipid Membranes -- Modeling Lipid Metabolism in Yeast -- Mycolic Acids: Structures, Biosynthesis , and Function -- Nonpolar Lipids in Yeast: Synthesis, Storage and Degradation -- Ornithine Lipids and Other Amino Acid-containing Acyloxyacyl Lipids -- Outer Membrane Extension Nanowires of Bacteria -- Outer Membrane Vesicles of Bacteria -- Phenolic Lipids Synthesized by Type III Polyketide Synthases -- Players in the Nonpolar Lipid Game - Proteins Involved in Nonpolar Lipid Metabolism in Yeast -- Polyhydroxyalkanoate Biogenesis -- Preface: Biogenesis of Fatty Acids, Lipids and Membranes -- Production of Wax Esters by Bacteria -- Protein Lipidation, Elucidation by Chemical Proteomics, and its Functional Roles -- Regulation of Membrane Lipid Homeostasis in Bacteria upon Temperature Change -- Role of Lipid Domains in Bacterial Cell Processes -- Role of Lipids in the Eukaryotic Secretory Pathway -- Role of the BAM Complex in Outer Membrane Assembly -- Structure:Function of Transmembrane Domains of Proteins and Transmembrane Organelles -- Synthesis of AcetylCoA from Carbon Dioxide in Acetogenic Bacteria -- Synthesis of Acetyl-CoA from Carbon Dioxide in Acetogenic Bacteria -- The Biosynthesis and Evolution of Archaeal Membranes and Ether Phospholipids -- Type III Polyketide Synthases Responsible for Phenolic Lipid Synthesis -- Vitamin Formation from Fatty Acid Precursors -- Wax Ester and Triacylglycerol Biosynthesis in Bacteria
    Abstract: Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes). All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes
    Pages: 161 illus., 115 illus. in color. eReference. : online resource.
    ISBN: 9783319504308
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  • 45
    Keywords: Emerging infectious diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Analysis of Iron Requirements and Siderophore Production -- The Pathometabolism of Legionella Studied by Isotopologue Profiling -- Quantification of Viable but Non-culturable Cells of Legionella pneumophila -- Selection of Legionella Virulence-related Traits by Environmental Protozoa -- Migration of Acanthamoeba castellanii through Legionella Biofilms -- Scar-free Genome Editing in Legionella pneumophila -- Querying Legionella Genomes using Transposition-sequencing -- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq -- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae -- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium -- Sorting of Phagocytic Cells Infected with Legionella pneumophila -- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment -- Mitochondrial Dynamics and Activity in Legionella-Infected Cells -- Perturbation of Legionella Cell Infection by RNA Interference -- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila -- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography -- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila -- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification -- Inflammasome Activation in Legionella-infected Macrophages -- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila -- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence -- Dictyostelium Host Response to Legionella Infection: Strategies and Assays -- 6.1.3 The Caenorhabditis elegans model of Legionella infection -- The Mouse as a Model for Pulmonary Legionella Infection -- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples -- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients -- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry
    Abstract: The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens
    Pages: XXVI, 476 p. 74 illus., 50 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990481
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  • 46
    Keywords: Oncology ; Immunology ; Cancer Research ; Immunology ; Springer eBooks
    Description / Table of Contents: Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry
    Abstract: This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies
    Pages: XI, 225 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493989799
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  • 47
    Keywords: Nucleic Acids ; Genetic Engineering ; Popular Life Sciences ; Nucleic Acid Chemistry ; Genetic Engineering ; Popular Science in Chemistry and Materials ; Springer eBooks
    Abstract: Carolyń€™s parents did not, after all, make genomics history by synthesizing her genome in a lab. She has known she is the "Human Hoax" ever since a high school genetics exercise revealed she has trisomy X€”á chromosomal abnormality€”ýet no synthetically constructed genome would have such clear traces of natural conception. Many years later, as molecular biologist, she hopes her colleagues never learn of her embarrassing origins. But when someone ransacks her office and lab, she finds professional embarrassment is the least of her worries. Someone believes she has the results of her parentś€™ last, secret experiments, and is willing to kill to get them. But all she has from her parents are their geneś€”can she find what else they may have left her before somebody else does? In a not-so-distant society, where corporations wield as much power as nations and the line between corporate employee and state authority is blurred, the chase is on. Carolyn may have just too little time at hand to unravel the mystery of her parentś€™ final days and to realize the deep consequences for the future of mankind. This fast-paced novel is followed by an extensive science chapter where the author provides a non-technical primer on modern genetics and on the speculative biology behind Carolyń€™s code. Dr V Anne Smith is on the biology faculty at the University of St Andrews in Scotland. She researches computational biology, concentrating on complex biological networks. She develops algorithms to infer functional interactions based upon observational data, generating biological discovery in areas as diverse as ecosystems and gene regulation. In addition to doing research, she enjoys writing science fiction stories. A Code for Carolyn is her first scientific novel
    Pages: V, 235 p. 6 illus. : online resource.
    ISBN: 9783030045531
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  • 48
    Keywords: Plant genetics ; Gene Expression ; Plant Genetics and Genomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants -- The Plant MicroRNA Pathway: The Production and Action Stages -- Plant miRNA Conservation and Evolution -- Bioinformatic Analysis of Small RNA Sequencing Libraries -- An Overview of miRNA and miRNA Target Analysis Tools -- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences -- Prediction of Plant miRNA Targets -- Isolation and Detection Methods of Plant miRNAs -- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants -- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses -- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization -- In Situ Localization of Small RNAs in Plants -- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs -- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy -- Degradome Sequencing in Plants -- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System -- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis -- Mimicry Technology: A Versatile Tool for Small RNA Suppression -- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants -- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches -- Analysis of Methylation Status of Plant MicroRNAs -- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 -- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs -- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae -- Detection of Histone Modifications Associated with miRNAs
    Abstract: This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science
    Pages: XII, 363 p. 70 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990429
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  • 49
    Keywords: Gene Therapy ; Medical virology ; Gene Therapy ; Virology ; Springer eBooks
    Description / Table of Contents: Basic Concepts in Viral Vector-Mediated Gene Therapy -- Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing -- Design, Construction, and Application of Transcription Activation-Like Effectors -- Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain -- AAV Production using Baculovirus Expression Vector System -- Multimodal Production of Adeno-Associated Virus -- Generation of High Titer Pseudotyped Lentiviral Particles -- A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration -- Current Use of Adenovirus Vectors and their Purification Methods -- Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest -- Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes -- AAV Mediated Gene Delivery to the Mouse Liver -- Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse -- Gene Transfer to Mouse Kidney In Vivo -- Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders -- Localized Intra-Arterial Gene Delivery using AAV -- Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors -- Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs -- Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons -- Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice -- Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates
    Abstract: This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments
    Pages: XIV, 328 p. 46 illus., 33 illus. in color. : online resource.
    ISBN: 9781493990658
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  • 50
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Introduction to Drug Testing – The Expanding Role of Mass Spectrometry -- Quantification of Eight Cannabinoids Including Cannabidiol in Human Urine Via Liquid Chromatography Tandem Mass Spectrometry -- Analysis of Benzodiazepines for Drug Facilitated Assaults and Abuse Settings (Urine) -- Targeted Opioid Screening Assay for Pain Management Using High Resolution Mass Spectrometry -- Measurement of Buprenorphine and Norbuprenorphine in Urine -- Quantitation of Tapentadol by Liquid Chromatography – Tandem Mass Spectrometry -- Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS -- LC-MS/MS method for the quantification of the Leflunomide metabolite, Teriflunomide, in Human Serum/Plasma -- Analysis of Tryptic Peptides from Therapeutic Monoclonal Antibodies using LC-MS/MS -- Quantification of Methotrexate in Human Serum and Plasma by Liquid Chromatography Tandem Mass Spectrometry -- Simultaneous Determination of Tacrolimus and Cyclosporine A in Whole Blood by Ultra-Fast LC-MS/MS -- Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) Method to Quantify Gabapentin and Pregabalin in Urine -- The Evolving Landscape of Designer Drugs -- Analysis of Synthetic Cannabinoid Metabolites by Liquid Chromatography-Tandem Mass Spectrometry -- Quantification of Designer Opioids by Liquid Chromatography Tandem Mass Spectrometry -- Screening Analysis for Designer Stimulants by LC-MS/MS -- Drug Screening Using Liquid Chromatography Quadrupole Time of Flight (LC-QqTOF) Mass Spectrometry -- Alternate Matrices – Meconium, Cord Tissue, Hair, and Oral Fluid -- Salt-assisted Liquid-Liquid Extraction of Meconium for Analysis of Cocaine and Amphetamines by Liquid Chromatography-tandem Mass Spectrometry -- Detection of In Utero Cannabis Exposure in Umbilical Cord Tissue by A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method -- Quantitation of Ethyl β-D-glucuronide in Human Umbilical Cord Tissue by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) -- Analysis of Drugs in Oral Fluid using LC-MS/MS -- Determination of Cocaine and Metabolites in Dried Blood Spots by LC-MS/MS
    Abstract: This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusing on advances in technology, alternate matrices, and rapidly-changing classes of drugs of abuse, compounds pertinent to toxicology, and therapeutic agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, LC-MS in Drug Analysis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field
    Pages: X, 274 p. 36 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988235
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  • 51
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) -- Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells -- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD -- Chromatin Immunoprecipitation in Skeletal Myoblasts -- Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor -- Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation -- Lentivirus-Mediated RNAi in Skeletal Myogenesis -- Adipogenesis from Bovine Precursors -- Transcriptomic Profiling during Myogenesis -- Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle -- Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System -- LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue -- siRNA Gene Silencing during C2C12 Myogenesis -- Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury -- Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis -- Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis -- Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers -- Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis -- Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies -- RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis
    Abstract: This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis
    Pages: XII, 351 p. 84 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988976
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  • 52
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Phytoplasmas: An Introduction -- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques -- Phytoplasma Transmission: Insect Rearing and Infection Protocols -- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors -- Symptoms of Phytoplasma Diseases -- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas -- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene -- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas -- Real-time PCR Protocol for Phytoplasma Detection and Quantification -- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves -- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas -- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas -- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification -- Quantitative Analysis with Droplet Digital PCR -- Rapid Sample Preparation and LAMP for Phytoplasma Detection -- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly -- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’ -- Transcriptomic Analyses of Phytoplasmas -- Transcriptomic Analyses of Phytoplasmas -- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study -- Collection of Phloem Sap in Phytoplasma-infected Plants -- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas -- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas -- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) -- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants -- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants
    Abstract: This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 362 p. 75 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988372