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  • Springer  (1,651,717)
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  • 1
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single Molecule FRET Methods to Study Glutamate Receptors -- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) -- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy -- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors -- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function -- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) -- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus -- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats -- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction -- Gene Expression Analysis by Multiplex Single Cell RT-PCR -- Metabolomics Analysis of Glutamate Receptor Function -- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation -- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function -- Fractionation of Subcellular Compartments from Human Brain Tissue -- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
    Abstract: This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications
    Pages: X, 260 p. 67 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990771
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  • 2
    Call number: WN180:8
    Keywords: Diagnostic Imaging ; Artificial intelligence
    Pages: xv, 373 p. : ill.
    ISBN: 9783319948775
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  • 3
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 4
    Keywords: Plant breeding ; Plant genetics ; Agriculture ; Genetic Engineering ; Alternative Medicine ; Pharmaceutical technology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Agriculture ; Genetic Engineering ; Complementary & Alternative Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Foreword by Prof. M. Iqbal Choudhary, Director, International Center for Chemical and Biological Sciences -- Marine and Freshwater Organisms in Drug Discovery -- Bioactive Molecules from Malaysian Flora -- Immunomosulatory Effect of Cycloartane-Type Triterpenes -- Antigiardial Actitivty of Citrullus lanatus Extracts -- Potential Idiosyncratic Liver Toxicity of Herbal Products -- Antitrypanosomal Activity of Nigerian Plants -- Alkaloids Isolated from Malaysian Plant Families -- Pharmacological Studies on Ficus Species -- Herbals in the treatment of Salmonella typhi -- Biology of Proline-Containing Cyclic Peptides -- Plant Extracts and Memory Impairment -- Herbals Used in Heat and Detoxification in Chinese Traditional Medicine -- Secondary Metabolites from Solanaceae of Iran -- Molecular Docking Study on Rauvolfia species -- Drug Development from the farm -- Phytochemical Screening of Sri Lankan Plants -- New carbohydrate esters of cinnamic acid from the leaves of Gleditschia -- Herbals in the Treatment of Alzheimer's disease
    Abstract: Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the “scientific methods”. Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, it will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture
    Pages: XX, 385 p. 67 illus., 45 illus. in color. : online resource.
    ISBN: 9783030044084
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  • 5
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 6
    Keywords: Biomedical Engineering ; Neurosciences ; Radiology, Medical ; Neurology ; Biomedical Engineering/Biotechnology ; Neurosciences ; Diagnostic Radiology ; Mathematical and Computational Biology ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Introduction -- Chapter 2. Multifractal study of EEG signal of subjects with Epilepsy and Alzheimer’s -- Chapter 3. Multifractal Approach for Quantification of Autonomic Deregulation due to Epileptic Seizure with ECG data -- Chapter 4. Multifractal Analysis of Electromyography data.-Chapter 5. Multifractal study on Parkinson’s and Huntington diseases with human gait data -- Chapter 6. Multifractal correlation study between posture and autonomic deregulation using ECG and blood pressure data -- Chapter 7. Appendix
    Abstract: This book primarily focuses on the study of various neurological disorders, including Parkinson’s (PD), Huntington (HD), Epilepsy, Alzheimer’s and Motor Neuron Diseases (MND) from a new perspective by analyzing the physiological signals associated with them using non-linear dynamics. The development of nonlinear methods has significantly helped to study complex nonlinear systems in detail by providing accurate and reliable information. The book provides a brief introduction to the central nervous system and its various disorders, their effects on health and quality of life, and their respective courses of treatment, followed by different bioelectrical signals like those detected by Electroencephalography (EEG), Electrocardiography (ECG), and Electromyography (EMG). In turn, the book discusses a range of nonlinear techniques, fractals, multifractals, and Higuchi’s Fractal Dimension (HFD), with mathematical examples and procedures. A review of studies conducted to date on neurological disorders like epilepsy, dementia, Parkinson’s, Huntington, Alzheimer’s, and Motor Neuron Diseases, which incorporate linear and nonlinear techniques, is also provided. The book subsequently presents new findings on neurological disorders of the central nervous system, namely Parkinson’s disease and Huntington’s disease, by analyzing their gait characteristics using a nonlinear fractal based technique: Multifractal Detrended Fluctuation Analysis (MFDFA). In closing, the book elaborates on several parameters that can be obtained from cross-correlation studies of ECG and blood pressure, and can be used as markers for neurological disorders
    Pages: XX, 182 p. 77 illus., 51 illus. in color. : online resource.
    ISBN: 9789811335525
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  • 7
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 8
    Keywords: Plant genetics ; Gene Expression ; Plant Genetics and Genomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants -- The Plant MicroRNA Pathway: The Production and Action Stages -- Plant miRNA Conservation and Evolution -- Bioinformatic Analysis of Small RNA Sequencing Libraries -- An Overview of miRNA and miRNA Target Analysis Tools -- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences -- Prediction of Plant miRNA Targets -- Isolation and Detection Methods of Plant miRNAs -- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants -- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses -- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization -- In Situ Localization of Small RNAs in Plants -- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs -- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy -- Degradome Sequencing in Plants -- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System -- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis -- Mimicry Technology: A Versatile Tool for Small RNA Suppression -- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants -- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches -- Analysis of Methylation Status of Plant MicroRNAs -- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 -- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs -- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae -- Detection of Histone Modifications Associated with miRNAs
    Abstract: This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science
    Pages: XII, 363 p. 70 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990429
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  • 9
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction -- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction -- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells -- Isolation and Characterization of Cutaneous Epithelial Stem Cells -- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin -- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells -- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis -- Isolation and Cultivation of Epidermal (Stem) Cells -- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen -- Isolation and Cultivation of Skin-Derived Precursors -- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells -- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor -- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair -- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet -- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering -- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration -- Skin Wound Healing: Refractory Wounds and Novel Solutions -- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice -- Melanoblasts as Multipotent Cells in Murine Skin -- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro -- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells -- Isolating Immune Cells from Mouse Embryonic Skin -- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells -- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy -- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications -- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis -- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells -- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research -- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma -- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells -- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
    Abstract: This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells
    Pages: XV, 463 p. 105 illus., 76 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988709
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  • 10
    Keywords: Oncology ; Immunology ; Cancer Research ; Immunology ; Springer eBooks
    Description / Table of Contents: Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry
    Abstract: This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies
    Pages: XI, 225 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493989799
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  • 11
    Keywords: Nucleic Acids ; Genetic Engineering ; Popular Life Sciences ; Nucleic Acid Chemistry ; Genetic Engineering ; Popular Science in Chemistry and Materials ; Springer eBooks
    Abstract: Carolyń€™s parents did not, after all, make genomics history by synthesizing her genome in a lab. She has known she is the "Human Hoax" ever since a high school genetics exercise revealed she has trisomy X€”á chromosomal abnormality€”ýet no synthetically constructed genome would have such clear traces of natural conception. Many years later, as molecular biologist, she hopes her colleagues never learn of her embarrassing origins. But when someone ransacks her office and lab, she finds professional embarrassment is the least of her worries. Someone believes she has the results of her parentś€™ last, secret experiments, and is willing to kill to get them. But all she has from her parents are their geneś€”can she find what else they may have left her before somebody else does? In a not-so-distant society, where corporations wield as much power as nations and the line between corporate employee and state authority is blurred, the chase is on. Carolyn may have just too little time at hand to unravel the mystery of her parentś€™ final days and to realize the deep consequences for the future of mankind. This fast-paced novel is followed by an extensive science chapter where the author provides a non-technical primer on modern genetics and on the speculative biology behind Carolyń€™s code. Dr V Anne Smith is on the biology faculty at the University of St Andrews in Scotland. She researches computational biology, concentrating on complex biological networks. She develops algorithms to infer functional interactions based upon observational data, generating biological discovery in areas as diverse as ecosystems and gene regulation. In addition to doing research, she enjoys writing science fiction stories. A Code for Carolyn is her first scientific novel
    Pages: V, 235 p. 6 illus. : online resource.
    ISBN: 9783030045531
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  • 12
    Keywords: Biomedical Engineering ; Biology / Data processing ; Biotechnology ; Biomedical Engineering/Biotechnology ; Computer Appl. in Life Sciences ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Enhancing Nursing Education through Affordable and Realistic Holographic Mixed Reality: The Virtual Standardized Patient for Clinical Simulation -- Potential Application Of Virtual Reality For Interface Customisation (And Pre-Training) Of Amputee Patients As Preparation For Prosthetic Use -- Visualising Medical Heritage: New Approaches To Digitisation And Interpretation Of Medical Heritage Collections -- Integrating 3D visualisation technologies in undergraduate anatomy education -- Pedagogical perspectives on the use of technology within medical curricula: moving away from norm driven implementation -- Towards a More User-Friendly Medication Information Delivery to People Living with Multiple Sclerosis: A Case Study with Alemtuzumab -- The Co-Design of Hand Rehabilitation Exercises for Multiple Sclerosis Using Hand Tracking System -- Examining Vascular Structure And Function Using Confocal Microscopy And 3D Imaging Techniques -- Which Tool Is Best: 3D Scanning Or Photogrammetry - It Depends On The Task -- Application of Photogrammetry in Biomedical Science
    Abstract: This edited volume explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in imaging and analysis, education, engagement and training. The reader will be able to explore the utilisation of technologies from a number of fields to enable an engaging and meaningful visual representation of the life sciences. This use of technology-enhanced learning will be of benefit for the learner, trainer, in patient care and the wider field of education and engagement. By examining a range of techniques in image capture (photogrammetery, stereophotogrammetry, microphotogrammetry and autostereoscopy), this book will showcase the wide range of tools we can use. Researchers in this field will be able to find something suitable to apply to their work to enhance user engagement through improved visual means using the technologies we have available to us today. It will highlight the uses of these technologies to examine many aspects of the human body, and enable improved ways to enhance visual and tactile learning, including 3D printing. By demonstrating co-design processes, working directly with the end-stage users (including patients), it will also highlight successes in adopting tools like hand motion tracking rehabilitation for patients with conditions like multiple sclerosis. The book will also discuss the applications of immersive environments including virtual, augmented and mixed reality. The ultimate aim is to show how, by using these tools, we can enhance communication, mobile applications, health literacy and illustration of both normal and pathological processes in the body. By applying a wide range of tools and technologies, this volume will highlight the wide range of applications in education, training and learning both for students and faculty, but also for patient care and education. Therefore, the work presented here can be accessed by a wide range of users from faculty and students involved in the design and development of these processes, by examining the pedagogy around these technologies. Importantly, it presents material, which will be of benefit for the patient, engaging them to become more involved with techniques like physiotherapy
    Pages: XV, 132 p. 65 illus., 42 illus. in color. : online resource.
    ISBN: 9783030060701
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  • 13
    Keywords: Microbiology ; Microbial genetics ; Microbial genomics ; Biomedical Engineering ; Eukaryotic Microbiology ; Microbial Genetics and Genomics ; Applied Microbiology ; Medical Microbiology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Industrial Applications and Model Systems -- Physiological genomics of multistress resistance in the yeast cell model and factory: focus on MDR/MXR transporters -- Mechanisms of yeast adaptation to wine fermentations -- Development of robust yeast strains for lignocellulosic bio-refineries based on genome-wide studies -- Physiological genomics of the highly weak-acid-tolerant food spoilage yeasts of Zygosaccharomyces bailii sensu lato -- Yeast genome-scale metabolic models for simulating genotype-phenotype relations -- Human Health and Disease -- Emerging mechanisms of drug resistance in Candida albicans -- Genome-wide response to drugs and stress in the pathogenic yeast Candida glabrata -- Lipidomics approaches: applied to the study of pathogenesis in Candida species -- Yeast at the forefront of research on ageing and age-related diseases
    Abstract: This book discusses genome-based strategies to provide a holistic understanding of yeasts in Human Health and as model organisms in basic research or industrial production. Using numerous Saccharomyces cerevisiae strains and various non-conventional yeast species isolated from diverse origins, it describes essential biological processes, the biotechnological exploitation of yeast and pathogenesis control. It also demonstrates how functional and comparative genomics and the development of genome engineering tools are used in modern yeast research. The use of yeasts as experimental eukaryotic models increasingly gained prominence when several Nobel Prizes in Physiology/Medicine and Chemistry were awarded for innovative research, using yeast strains to elucidate molecular mechanisms in a wide range of human physiological processes and diseases, such as autophagy, cell cycle regulation and telomerase activity. This book offers useful insights for scientists in yeast research, clinical scientists working with yeast infectious models and for industrial researchers using applied microbiology
    Pages: V, 242 p. 37 illus., 29 illus. in color. : online resource.
    ISBN: 9783030130350
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  • 14
    Keywords: Entomology ; Plant breeding ; Plant anatomy ; Biomedical Engineering ; Mycology ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Preface -- Biodiversity of Fungi from Extreme Habitats and Its Industrial Applications -- Rhizospheric Fungi Isolated from Different Plants and Potential Applications in Diverse Sectors -- Endophytic Fungi from Different Niches with Potential Industrial Importance -- Marine Fungal White Biotechnology -- Aspergillus Biodiversity, Ecological Significance, and Industrial Applications -- Fusarium Biodiversity, Ecological Significance, and Industrial Applications -- Penicillium Biodiversity, Ecological Significance, and Industrial Applications -- Trichoderma Biodiversity, Ecological Significance, and Industrial Applications -- Thermostable Enzymes from Fungi Isolated from Hot Springs and Its Applications -- Cold Active Enzymes from Psychrophilic Fungi and Its Applications -- Industrially Important Enzymes from White-Rot Fungi or Filamentous Fungi -- Fungal Enzymes for the Textile Industry -- Fungal Laccase -- Fermentation Process for Production of Fungal Cellulases -- Fungal Lipases -- Fungal Community and Eco-Friendly Bioresources for Novel Secondary Metabolites -- Agriculturally Important Fungi -- Fungal White Biotechnology in Biosurfactants -- Synthetic Biology and a Novel Approach for Pharmaceutically Important Compounds -- Global Scenario of Fungal White Biotechnology -- Appendixes -- Index --
    Abstract: White biotechnology, or industrial biotechnology as it is also known, refers to the use of living cells and/or their enzymes to create industrial products that are more easily degradable, require less energy, create less waste during production and sometimes perform better than products created using traditional chemical processes. Over the last decade considerable progress has been made in white biotechnology research, and further major scientific and technological breakthroughs are expected in the future. Fungi are ubiquitous in nature and have been sorted out from different habitats, including extreme environments (high temperature, low temperature, salinity and pH), and may be associated with plants (epiphytic, endophytic and rhizospheric). The fungal strains are beneficial as well as harmful for human beings. The beneficial fungal strains may play important roles in the agricultural, industrial, and medical sectors. The fungal strains and their products (enzymes, bioactive compounds, and secondary metabolites) are very useful for industry (e.g., the discovery of penicillin from Penicillium chrysogenum). This discovery was a milestone in the development of white biotechnology as the industrial production of penicillin and antibiotics using fungi moved industrial biotechnology into the modern era, transforming it into a global industrial technology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors, providing both high value nutraceutical and pharmaceutical products. The fungal strains and bioactive compounds also play an important role in environmental cleaning. This volume covers the latest developments and research in white biotechnology with a focus on diversity and enzymes
    Pages: XIX, 571 p. 108 illus., 61 illus. in color. : online resource.
    ISBN: 9783030104801
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  • 15
    Keywords: Stem Cells ; Ophthalmology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Ophthalmology ; Springer eBooks
    Description / Table of Contents: Advantages of the Eye as a Target Organ for Cell-Based Therapy in the Central Nervous System -- Proof of Principle: Preclinical Data on Retinal Cell Transplantation -- Clinical and Pathological Features of Selected Human Retinal Degenerative Diseases -- Degenerative Retinal Diseases: Cells sources for cell-based therapy -- Manufacturing of Clinical Grade Cellular Products Under Gmp Conditions -- Regulatory Requirements for Cell-Based Therapy for Degenerative Retinal Disease -- Biomaterials and Scaffolds for Cell Replacement Therapy -- Restoration of cone photoreceptor function in retinitis pigmentosa (RP) – retinal cell-based therapy -- Cell Delivery: Surgical Approaches -- Diagnosis and Complementary Examinations -- Cell Therapy for Degenerative Retinal Disease: special focus on cell fusion-mediated regeneration -- Clinical Trials of Retinal Cell Therapy -- Future Directions -- Index
    Abstract: This book discusses why specific diseases are being targeted for cell-based retinal therapy, what evidence exists that justifies optimism for this approach, and what challenges must be managed in order to bring this technology from the laboratory into routine clinical practice. There are a number of unanswered questions (e.g., surgical approach to cell delivery, management of immune response, optimum cell type to transplant) that very likely are not going to be answered until human trials are undertaken, but there is a certain amount of “de-risking” that can be done with preclinical experimentation. This book is essential reading for scientists, clinicians, and advanced students in stem cell research, cell biology, and ophthalmology
    Pages: XI, 280 p. 27 illus., 20 illus. in color. : online resource.
    ISBN: 9783030052225
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  • 16
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    Singapore : Springer
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Evolution (Biology) ; Biodiversity ; Drug Interactions ; Microbial Genetics and Genomics ; Microbial ecology ; Evolutionary Biology ; Biodiversity ; Drug Resistance ; Springer eBooks
    Description / Table of Contents: Bacteria -- Functions of archaeal nucleoid proteins: archaeal silencers are still missing -- Acquired and innate immunity in prokaryote define their evolutionary story -- Mobile genetic elements -- Viruses of the archaea and evolution of life -- Overlooked “broad-host range vector particles” (VPs) in the environ -- Bacteria-virus interaction -- Plasmids and their hosts -- Bacterial transposable elements -- RNA-mediated crosstalk between bacterial host genome and foreign genetic elements -- Extracellular DNA in seawater and marine sediment -- Acquiring phenotypic diversification on genomic diversification
    Abstract: This book comprehensively discusses our current understanding of the role and biological mechanisms of horizontal transfer of genetic elements in the environment, which has been important in the evolution of prokaryotes (archaea and bacteria). Horizontal transfer of genetic elements generates variations of prokaryotes and their genomes. Comparative studies of genomes revealed that it frequently occurred during archaeal and bacterial evolution. The book introduces a variety of studies related to horizontal gene transfer, gene silencing, plasmids, phages, transposons, and the emergence of microbes that degrade man-made xenobiotics and have antimicrobial resistance. Written by leading researchers in DNA traffic, the book is a valuable guide to horizontal transfer for both young scientists and experts in the field
    Pages: VII, 278 p. 39 illus., 25 illus. in color. : online resource.
    ISBN: 9789811334115
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  • 17
    Keywords: Mathematics ; Mathematics Education ; History of Mathematical Sciences ; International and Comparative Education ; Springer eBooks
    Description / Table of Contents: Chapter 1. European Didactic Traditions in Mathematics: Introduction and Overview -- Chapter 2. The French Didactic Tradition in Mathematics -- Chapter 3. Didactics of mathematics in the Netherlands -- Chapter 4. The Italian Didactic Tradition.-Chapter 5. The German Speaking Didactic Tradition -- Chapter 6. Didactics of Mathematics as a Research Field in Scandinavia -- Chapter 7. Czech and Slovak Research in Didactics of Mathematics Tradition and a Glance at Present State
    Abstract: This open access book discusses several didactic traditions in mathematics education in countries across Europe, including France, the Netherlands, Italy, Germany, the Czech and Slovakian Republics, and the Scandinavian states. It shows that while they all share common features both in the practice of learning and teaching at school and in research and development, they each have special features due to specific historical and cultural developments. The book also presents interesting historical facts about these didactic traditions, the theories and examples developed in these countries
    Pages: V, 212 p. 28 illus., 10 illus. in color. : online resource.
    ISBN: 9783030055141
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  • 18
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Origin and Pathogenesis of B Cell Lymphomas -- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma -- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells -- PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations -- Expression Cloning of Antibodies from Single Human B Cells -- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR -- Stereotyped B Cell Immunoglobulins in B Cell Lymphomas -- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies -- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR -- Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input -- FISH and FICTION in Lymphoma Research -- NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma -- RNA Sequencing in B-Cell Lymphomas -- Studying Cancer Heterogeneity by Single Cell RNA-Sequencing -- Evaluation of Whole Genome Sequencing Data -- Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines -- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens -- Proximity Ligation Assay -- Phospoproteomic Analysis of Signaling Pathways in Lymphomas -- Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries
    Abstract: This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology
    Pages: XII, 439 p. 66 illus., 48 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991518
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  • 19
    Keywords: Biomedical Engineering ; Microbiology ; Biomedical Engineering/Biotechnology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Pathway and Gene Discovery from Natural Hosts and Organisms -- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity -- Antisense RNA Elements for Downregulating Expression -- Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering -- Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs -- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae -- Assembly of Complex Pathways using Type IIs Restriction Enzymes -- Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly -- Checks and Balances with Use of the Keio Collection for Phenotype Testing -- Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803 -- Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica -- Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization -- Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization -- A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models -- 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms -- A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast
    Abstract: This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications
    Pages: X, 252 p. 46 illus., 39 illus. in color. : online resource.
    ISBN: 9781493991426
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  • 20
    Keywords: Biotechnology ; Biochemistry ; Biotechnology ; Protein Science ; Springer eBooks
    Description / Table of Contents: Established and Upcoming Yeast Expression Systems -- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts -- Pichia pastoris Promoters -- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes -- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha -- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica -- High-throughput Screening and Selection of Pichia pastoris Strains -- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke -- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori -- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae -- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast -- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production -- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments -- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris -- Efficient Development of a Mixed Feed Process for Pichia pastoris -- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification -- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants -- LC-MS analysis of (glyco)-proteins of Pichia pastoris -- Assessing the Quality of Recombinant Products Made in Yeast -- Quantification of Recombinant Products In Yeast
    Abstract: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications
    Pages: XII, 431 p. 60 illus., 46 illus. in color. : online resource.
    ISBN: 9781493990245
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  • 21
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    New York : Springer
    Keywords: Medicine ; Orthopedics ; Molecular Medicine ; Orthopedics ; Springer eBooks
    Description / Table of Contents: Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone
    Abstract: This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research
    Pages: XV, 670 p. 172 illus., 121 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493989973
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  • 22
    Keywords: Pharmaceutical technology ; Pharmacy ; Nanochemistry ; Pharmaceutical Sciences/Technology ; Pharmacy ; Nanochemistry ; Springer eBooks
    Description / Table of Contents: Understanding the Surface Characteristics of Nanoparticles -- Surface Modification of Nanoparticles for Targeted Drug Delivery -- Surface-Modified PLGA Nanoparticles for Targeted Drug Delivery to Neurons -- Nanoparticles for Site Specific Drug Delivery -- Stealth Properties of Nanoparticles against Cancer: Surface Modification of NPs for Passive Targeting to Human Cancer Tissue in Zebra Fish -- Passive targeting of nanoparticles to cancer -- Surface Modification of Magnetic Nanoparticles in Biomedicine -- A novel strategy for the surface modification of super paramagnetic (Fe3O4) iron oxide nanoparticle for lung cancer imaging -- Biodegradable nanoparticles for drug delivery and targeting -- Functionalized antibacterial nanoparticles in controlling biofilm and intracellular infections -- Surface modifications of liposomes for drug targeting -- Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system -- Surface Modification of Metallic Nano particles -- Successful Delivery of Zidovudine-loaded Docosanol Nanostructured Lipid Carriers (Docosanol NLCs) into rat brain -- Brain targeted drug delivery with surface modified nanoparticles -- Surface Modification of Resorcinarene Based Self-Assembled Solid Lipid Nanoparticles for Drug Targeting -- Targeted delivery of surface modified nanoparticles:Modulation of inflammation for acute lung injury -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface Modification of Gold Nanoparticles for Targeted Drug Delivery -- Surface modification and bioconjugation of nanoparticles for MRI technology -- Surface Modified Lanthanide Nanomaterials for Drug Delivery -- Engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers -- Application of Nanoparticles in treating Periodontitis: Preclinical and Clinical overview -- Surface Modification of Nanoparticles for Ocular Drug Delivery
    Abstract: This unique book discusses various new techniques developed to enhance the application of nanoparticulate drug delivery systems using surface modification of nanoparticles. The understanding of the surface characteristics of nanoparticles is growing significantly with the advent of new analytical techniques. Polymer chemistry is contributing to the development of many new versatile polymers which have abilities to accommodate many different, very reactive chemical groups, and can be used as a diagnostic tool, for better targeting, for more effective therapeutic results as well as for reducing the toxic and side effects of the drugs. Surface modification of such polymeric nanoparticles has been found by many scientists to enhance the application of nano-particles and also allows the nanoparticles to carry specific drug molecule and disease /tumor specific antibodies which refine and improve drug delivery. Surface Modification of Nanoparticles for Targeted Drug Delivery is a collection of essential information on various applications of surface modification of nano-particles and their disease specific applications for therapeutic purposes
    Pages: XVIII, 499 p. 142 illus., 110 illus. in color. : online resource.
    ISBN: 9783030061159
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  • 23
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 24
    Keywords: Bacteriology ; Enzymes ; Immunology ; Bacteriology ; Enzymology ; Immunology ; Springer eBooks
    Description / Table of Contents: 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus
    Abstract: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets
    Pages: XIII, 312 p. 70 illus., 41 illus. in color. : online resource.
    ISBN: 9781493991549
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  • 25
    Keywords: Biochemistry ; Cytology ; Protein Science ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cloning and Mutagenesis Strategies for Large Collagens -- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen -- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds -- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils -- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy -- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry -- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes -- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics -- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy -- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology -- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics -- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix -- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans -- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis -- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment -- FACS Analysis of Col1α Protein Levels in Primary Fibroblasts -- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors
    Abstract: This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study
    Pages: XI, 254 p. 60 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990955
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  • 26
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: An Overview of CE in Clinical Analysis -- Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies -- Monitorization of α1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF Detection -- Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic Injection -- On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin -- Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis -- The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection -- Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization -- A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of Experiment -- New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS) -- Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Sample -- Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis -- Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis -- Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions -- A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood -- Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA) -- Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis -- Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen -- Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins
    Abstract: This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research
    Pages: XII, 273 p. 74 illus., 51 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992133
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  • 27
    Keywords: Genetics ; Human Genetics ; Medical virology ; Medicine ; Genetics and Genomics ; Human Genetics ; Virology ; Learning & Instruction ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Monogenic Disorders -- Cytogenetics -- Medical Genetics -- Viral Gene Therapy
    Abstract: This textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The “Labster Virtual Lab Experiments” series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn’t have access to. In this book, you’ll learn the fundamental concepts of the genetics of human diseases focusing on: Monogenic Disorders - Cytogenetics - Medical Genetics - Viral Gene Therapy In each chapter, you’ll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you’ll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access to five simulations for the duration of six months. If you’re using the e-book version, you can sign up and buy access to the simulations at www.labster.com/springer. If you like this book, try out other topics in this series, including “Basic Biology”, “Basic Genetics”, and “Basic Biochemistry”. About Labster Labster is a company dedicated to developing virtual lab simulations that are designed to stimulate students’ natural curiosity and highlight the connection between science and the real world. These simulations have been shown to improve the achievement of learning outcomes among students, by making the learning experience more immersive and engaging. The content of this book was created by the Labster team members Dr. Aaron Gardner, Dr. Sarah Stauffer, Dr. Lindsay Petley-Ragan, Philip Wismer, Dewi Ayu Kencana Ungu and Silvia Tjong. You can learn more about Labster and explore the simulations at www.labster.com
    Pages: IX, 76 p. 43 illus. in color. : online resource.
    ISBN: 9783662587447
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  • 28
    Keywords: Oncology ; Neurosciences ; Toxicology ; Psychiatry ; Cancer Research ; Neurosciences ; Pharmacology/Toxicology ; Psychiatry ; Springer eBooks
    Description / Table of Contents: 1. DUSP3/VHR: A Druggable Dual Phosphatase for Human Diseases -- 2. Oncotic Cell Death in Stroke -- 3. Magnesium Extravaganza: A Critical Compendium of Current Research into Cellular Mg2+ Transporters Other than TRPM6/7 -- 4. Curcumin in Advancing Treatment for Gynecological Cancers with Developed Drug- and Radiotherapy-Associated Resistance -- 5. Correction to Curcumin in Advancing Treatment for Gynecological Cancers with Developed Drug- and Radiotherapy-Associated Resistance
    Abstract: Lucas Falcão Monteiro, Pault Yeison Minaya Ferruzo, Lilian Cristina Russo, Jessica Oliveira Farias, and Fábio Luís Forti: DUSP3/VHR: A Druggable Dual Phosphatase for Human Diseases Kep Yong Loh, Ziting Wang, and Ping Liao: Oncotic Cell Death in Stroke Martin Kolisek, Gerhard Sponder, Ivana Pilchova, Michal Cibulka, Zuzana Tatarkova, Tanja Werner, and Peter Racay: Magnesium Extravaganza: A Critical Compendium of Current Research into Cellular Mg2+ Transporters Other than TRPM6/7 Amir Abbas Momtazi-Borojeni, Jafar Mosafer, Banafsheh Nikfar, Mahnaz Ekhlasi-Hundrieser, Shahla Chaichian, AbolfazlMehdizadehkashi,and Atefeh Vaezi: Curcumin in advancing treatment for gynecological cancers with developed drug- and radiotherapy-associated resistance Amir Abbas Momtazi-Borojeni, Jafar Mosafer, Banafsheh Nikfar, Mahnaz Ekhlasi-Hundrieser, Shahla Chaichian, AbolfazlMehdizadehkashi,and Atefeh Vaezi: Correction to: Curcumin in advancing treatment for gynecological cancers with developed drug- and radiotherapy-associated resistance
    Pages: V, 131 p. 14 illus., 13 illus. in color. : online resource.
    ISBN: 9783030140274
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  • 29
    Keywords: Proteomics ; Medical genetics ; Diabetes ; Proteomics ; Gene Function ; Diabetes ; Springer eBooks
    Description / Table of Contents: 1. Insulin Resistance in Schizophrenia -- 2. Biogenesis of the Insulin Secretory Granule in Health and Disease -- 3. Current Models of Fatty Liver Disease; New Insights, Therapeutic Targets and Interventions -- 4. Preclinical Models of Altered Early Life Nutrition and Development of Reproductive Disorders in Female Offspring -- 5. Targeting Mitochondrial Defects to Increase Lngevity in Animal Models of Neurodegenerative Diseases -- 6. Computational Approaches for Identification of Pleiotropic Biomarker Profiles in Psychiatry -- 7. MiRNA Regulation of Glucose and Lipid Metabolism in Relation to Diabetes and Non-Alcoholic Fatty Liver Disease -- 8. Hypercaloric Diet-Induced Obesity and Obesity-Related Metabolic Disorders in Experimental Models -- 9. Metabolic Biomarkers in Nematode C. elegans During Aging -- 10. Circular RNAs as potential biomarkers and therapeutic targets for metabolic diseases -- 11. Deciphering Endothelial Dysfunction in the HIV-Infected Population -- 12. The Role of Inflammation in the Development of GDM and The Use of Markers of Inflammation in GDM Screening -- 13. PFKP Signaling at a Glance: an Emerging Mediator of Cancer Cell Metabolism -- 14. ATAD3A on the Path to Cancer -- 15. Impact of Exercise on Inflammatory Mediators of Metabolic and Vascular Insulin Resistance in Type 2 Diabetes
    Abstract: The book recognizes that throughout the scientific, medical, and economic communities, new tests incorporating biomarkers are needed to improve the diagnosis of patients suffering from metabolic disorders. The early identification of those at risk of developing obesity will help to place these individuals on the best treatment course as early as possible for improved treatment outcomes. This will also help to cut costs incurred by the healthcare services. For all of this to occur, new research efforts are needed to identify novel biomarkers that can be used to predict the disease in the presymptomatic stage, for disease monitoring and for prediction of treatment response. It is also possible that new drug targets can be identified using these approaches which, in turn, can lead to the development of new treatment approaches. This volume also includes a series of reviews on biomarker discovery and usage in the study of diseases marked by perturbations in metabolism. It will describe the pros and cons of the various approaches and cover the successes and failures in this important research field
    Pages: XI, 303 p. 39 illus., 22 illus. in color. : online resource.
    ISBN: 9783030126681
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  • 30
    Keywords: Biomedical Engineering ; Cytology / Research ; Microbial ecology ; Microbiology ; Plant Diseases ; Biomedical Engineering/Biotechnology ; Biological Techniques ; Microbial ecology ; Microbiology ; Plant Pathology ; Springer eBooks
    Description / Table of Contents: Part I: Root symbioses -- Chapter 1. Synthetic plasmids to challenge symbiotic nitrogen fixation between rhizobia and legumes -- Chapter 2. In vivo analysis of rhizosphere enzyme activities by the use of plastic syringes -- Chapter 3. Characterization of arbuscular mycorrhizal communities in roots of vineyard plants -- Chapter 4. Molecular methods for research on actinorhiza -- Chapter 5. Molecular and functional characterization of beneficial bacteria associated with AMF spores -- Part II: Plant pathogens and microbial plant protection -- Chapter 6. Oomycete-root interactions -- Chapter 7. Exploitation of rhizosphere microbiome services -- Chapter 8. Methods for detecting biocontrol and plant growth-promoting traits in rhizobacteria -- Chapter 9. A split-root method to study systemic and heritable traits induced by Trichoderma in tomato plants -- Part III: Experimental approaches and analytical techniques in rhizosphere biology -- Chapter 10. Using a tri-isotope (13C, 15N, 33P) labelling method to quantify rhizodeposition -- Chapter 11. Microscopic techniques coupled to molecular and genetic approaches to highlight cell-type specific differences in mycorrhizal symbiosis -- Chapter 12. From imaging to functional traits in interactions between roots and microbes -- Chapter 13. Live imaging of arbuscular mycorrhizal symbiosis -- Chapter 14. Microcosm approaches to investigate multitrophic interactions in microbial communities -- Chapter 15. Analysis of common mycorrhizal networks in microcosms
    Abstract: This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation
    Pages: IX, 279 p. 37 illus., 34 illus. in color. : online resource.
    ISBN: 9789811357671
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  • 31
    Keywords: Bioinformatics ; Biomedical Engineering ; Bioinformatics ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: A Pycellerator Tutorial -- Using RuleBuilder to Graphically Define and Visualize BioNetGen-Language Patterns and Reaction Rules -- Strategy-Driven Exploration for Rule-Based Models of Biochemical Systems with PORGY -- Using rxncon to Develop Rule-Based Models -- Efficiently Encoding Complex Biochemical Models with the Multistate Model Builder (MSMB) -- Multi-Level Modeling and Simulation of Cellular Systems: An Introduction to ML-Rules -- Using Python for Spatially Resolved Modeling with Simmune -- Rule-Based Modeling using Wildcards in the Smoldyn Simulator -- MCell-R: A Particle-Resolution Network-Free Spatial Modeling Framework -- Spatial Rule-Based Simulations: the SRSim Software -- CellOrganizer: Learning and Using Cell Geometries for Spatial Cell Simulations -- Using Mechanistic Models for Analysis of Proteomic Data -- Annotations for Rule-Based Models -- Markov Chain Aggregation and Its Application to Rule-Based Modelling -- In Silico Evolution of Signaling Networks Using Rule-Based Models: Bistable Response Dynamics -- Recipes for Analysis of Molecular Networks Using the Data2Dynamics Modeling Environment -- RKappa: Software for Analyzing Rule-Based Models -- A Step-by-Step Guide to Using BioNetFit
    Abstract: This volume covers a variety of topics related to the practice of rule-based modeling, a type of mathematical modeling useful for studying biomolecular site dynamics. There is an emphasis on software tools and detailed descriptions of techniques. The chapters in this book discuss topics such as software tools and frameworks for compartmental modeling (Pycellerator, RuleBuilder, Prgy, rxncon, MSMB, and ML-Rules); tools for spatial modeling (Simmune, Smoldyn, MCell-R, SRSim, and CellOrganizer); rule-based models to analyze proteomic data; model annotation; Markov chain aggregation; BioJazz; and methods to identify model parameters (Data2Dynamics, RKappa, and BioNetFit). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary resources, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Biomolecular Site Dynamics: Methods and Protocols is a valuable resource for both the novice and expert rule-based modeler. It will also appeal to systems biologists and help them enhance their studies with easy-to-read and write rule-based models
    Pages: XIX, 423 p. 188 illus., 108 illus. in color. : online resource.
    ISBN: 9781493991020
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  • 32
    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media -- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell -- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell -- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome -- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host -- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome -- Gene Expression Analysis in the Pneumococcus -- Transcriptional Knockdown in Pneumococci using CRISPR Interference -- Protein Expression Analysis by Western Blot and Protein-Protein Interactions -- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony -- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria -- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells -- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae -- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease -- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection -- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo -- The Pneumococcus and Its Critical Role in Public Health -- The Epidemiology and Biostatistics of Pneumococcus
    Abstract: This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field
    Pages: XV, 227 p. 37 illus., 26 illus. in color. : online resource.
    ISBN: 9781493991990
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    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions -- Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c -- Value of DSC in Characterization and Optimization of Protein Stability -- Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC) -- Intrinsic Thermodynamics of Protein – Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design -- Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions -- ITC Studies of Ribosome/Antibiotics Interactions -- High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry -- ITC Measurement for High Affinity Aptamers Binding to their Target Proteins -- Thermodynamics of Molecular Machines using Incremental ITC -- Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry -- Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC) -- Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC -- Tinkering with Binding Polynomials in Isothermal Titration Calorimetry -- Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine -- Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter -- Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry -- Characterization of Enzymatic Reactions using ITC
    Abstract: This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 268 p. 60 illus., 40 illus. in color. : online resource.
    ISBN: 9781493991792
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  • 34
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 36
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Protocol to Compare Methods for Untargeted Metabolomics -- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry -- Measuring the Nutrient Metabolism of Adherent Cells in Culture -- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells -- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice -- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro -- Measuring Rates of ATP Synthesis -- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay -- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers -- Determining Compartment Specific Metabolic Fluxes -- Determining the Impact of Metabolic Nutrients on Autophagy -- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism -- Determining Macrophage Polarization upon Metabolic Perturbation -- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells -- Development of Patient-derived Tumor Xenograft Models -- Imaging Glioma Progression by Intravital Microscopy -- Lipectomizing Mice for Applications in Metabolism -- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants -- Determining the Intracellular Organization of Organelles -- The Fundamentals of Constructing and Interpreting Heat Maps
    Abstract: This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level
    Pages: XII, 294 p. 67 illus., 53 illus. in color. : online resource.
    ISBN: 9781493987696
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    New York, NY : Springer
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Molecular Dynamics Simulations of the SNARE Complex -- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory -- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates -- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion -- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins -- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly -- Studying Munc18:Syntaxin Interactions using Small-angle Scattering -- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion -- SNAP25 S-Guanylation and SNARE Complex Formation -- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion -- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery -- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery -- Determination of Sec18-Lipid Interactions by Liposome Binding Assay -- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1 -- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins -- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles -- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution -- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs -- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion -- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins -- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion -- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion -- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET and FCCS -- Visualization of SNARE-Mediated Organelle Membrane Hemifusion By Electron Microscopy -- Studies of the Secretory Machinery Dynamics by Total Internal Reflection Fluorescence Microscopy in Bovine Adrenal Chromaffin Cells -- Imaging SNAP-29 in Drosophila
    Abstract: This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field
    Pages: XIV, 407 p. 74 illus., 66 illus. in color. : online resource.
    ISBN: 9781493987603
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  • 38
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinsoń€™s Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- ℗Ư℗ƯMonitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the €œáutophagic flux́€ in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations
    Abstract: This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XVII, 741 p. 137 illus., 99 illus. in color. : online resource.
    ISBN: 9781493988730
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  • 39
    Keywords: Toxicology ; Laboratory animals ; Pharmacology/Toxicology ; Animal Models ; Springer eBooks
    Description / Table of Contents: A Guide to Mass Spectrometry-based Quantitative Proteomics -- Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes -- The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents -- The Forced Swim Test for Depression-like Behaviour in Rodents -- Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice -- The Nest Building Test in Mice for Assessment of General Well-being -- Mouse Free Dyadic Social Interaction Test -- The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour -- The Y-maze for Assessment of Spatial Working and Reference Memory in Mice -- Maturation of a Human Oligodendrocyte Cell Line -- Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan -- Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur -- Characterization of an Animal Model of Autism and Social Interaction -- Assaying Reproductive Capacity in Female Rodents -- Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents -- An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry -- Characterization of the db/db Mouse Model of Type 2 Diabetes -- Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes -- Characterization of Human Transplantable Insulinoma Cells -- Preparation of Islets from Rat Pancreas and Assessment of Islet Function -- Cryopreservation of Red Blood Cells -- Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation -- Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors -- The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis -- Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research -- The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity -- OVA-induced Allergic Airway Inflammation Mouse Model -- Description of Ovariectomy Protocol in Mice -- A Rat Eye Lens Model of Cataract Formation -- A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue -- Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line– a Preclinical Model for Type 2 Diabetes
    Abstract: This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas
    Pages: XIII, 343 p. 90 illus., 73 illus. in color. : online resource.
    ISBN: 9781493989942
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  • 40
    Keywords: Pharmaceutical technology ; Biochemistry ; Bioinformatics ; Pharmaceutical Sciences/Technology ; Medicinal Chemistry ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: Methods for Discovering and Targeting Druggable Protein-Protein Interfaces and Their Application to Repurposing -- Performing an In Silico Repurposing of Existing Drugs by Combining Virtual Screening and Molecular Dynamics Simulation -- Repurposing Drugs Based on Evolutionary Relationships between Targets of Approved Drugs and Proteins of Interest -- Drug Repositioning by Mining Adverse Event Data in ClinicalTrials.gov -- Transcriptomic Data Mining and Repurposing for Computational Drug Discovery -- Network-Based Drug-Repositioning: Approaches, Resources, and Research Directions -- A Computational Bipartite-Graph-Based Drug Repurposing Method -- Implementation of a Pipeline Using Disease-Disease Associations for Computational Drug Repurposing -- An Application of Computational Drug Repurposing Based on Transcriptomic Signatures -- Drug-Induced Expression-Based Computational Repurposing of Small Molecules Affecting Transcription Factor Activity -- A Drug Repurposing Method Based on Drug-Drug Interaction Networks and Using Energy Model Layouts -- Integrating Biological Networks for Drug Target Prediction and Prioritization -- Using Drug Expression Profiles and Machine Learning Approach for Drug Repurposing -- Computational Prediction of Drug-Target Interactions via Ensemble Learning -- A Machine-Learning-Based Drug Repurposing Approach Using Baseline Regularization -- Machine Learning Approach for Predicting New Uses of Existing Drugs and Evaluation of Their Reliabilities -- A Drug-Target Network-Based Supervised Machine Learning Repurposing Method Allowing the Use of Multiple Heterogeneous Information Sources -- Heter-LP: A Heterogeneous Label Propagation Method for Drug Repositioning -- Tripartite Network-Based Repurposing Method Using Deep Learning to Compute Similarities for Drug-Target Prediction
    Abstract: This detailed book explores techniques commonly used for research into drug repurposing, a well-known strategy to find alternative indications for drugs which have already undergone toxicology and pharma-kinetic studies but have failed later stages during the development, via computational methods. Thereby, it addresses the intense challenges of identifying the appropriate type of algorithm and relevant technical information for computational repurposing. Written for the highly successful Methods in Molecular Biology series, the authors of each chapter use their experience in the field to describe the implementation and successful use of a specific repurposing method thus providing lab-ready instruction. Authoritative and practical, Computational Methods for Drug Repurposing serves as an ideal guide to researchers interested in this vital area of drug development
    Pages: XI, 332 p. 103 illus., 82 illus. in color. : online resource.
    ISBN: 9781493989553
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  • 41
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Cheminformatics Approaches to Study Drug Polypharmacology -- Computational Predictions for Multi-Target Drug Design -- Computational Multi-Target Drug Design -- Multi-Target Drug Design for Neurodegenerative Diseases -- Molecular Docking Studies in Multi-Target Antitubercular Drug Discovery -- Advanced Chemometric Modeling Approaches for the Design of Multi-Target Drugs against Neurodegenerative Diseases -- Computational Studies on Natural Products for the Development of Multi-Target Drugs -- Computational Design of Multi-Target Drugs against Alzheimer’s Disease -- Design of Multi-Target Directed Ligands as a Modern Approach for the Development of Innovative Drug Candidates for Alzheimer’s Disease -- Virtual Screening for Dual Hsp90/B-Raf Inhibitors -- Strategies for Multi-Target Directed Ligands: Application in Alzheimer's Disease (AD) Therapeutics -- Computational Design of Multi-Target Kinase Inhibitors -- Proteochemometrics for the Prediction of Peptide Binding to Multiple HLA Class II Proteins -- Linked Open Data: Ligand-Transporter Interaction Profiling and Beyond -- Design of Novel Dual Target Hits against Malaria and Tuberculosis Using Computational Docking -- Computational Design of Multi-Target Drugs against Breast Cancer -- Computational Methods for Multi-Target Drug Designing against Mycobacterium tuberculosis -- Development of a Web-Server for Identification of Common Lead Molecules for Multiple Protein Targets -- Computational Method for Prediction of Targets for Breast Cancer Using siRNAs Approach -- Historeceptomics: Integrating a Drug's Multiple Targets (Polypharmacology) with Their Expression Pattern in Human Tissues -- Networking of Smart Drugs: A Chem-Bioinformatic Approach to Cancer Treatment
    Abstract: This detailed book showcases recent advances in computational design of multi-target drug candidates involving various ligand and structure-based strategies. Different chem-bioinformatic modeling strategies that can be applied for design of multi-target drugs as well as important databases and web servers in connection with multi-target drug design are also featured in this collection. Written for the Methods in Pharmacology and Toxicology series, chapters include the kind of key implementation advice that will aid researchers greatly in their laboratory pursuits. Authoritative and practical, Multi-Target Drug Design Using Chem-Bioinformatic Approaches seeks to aid all scientists working in the field of drug discovery research
    Pages: XVI, 563 p. 100 illus., 90 illus. in color. : online resource.
    ISBN: 9781493987337
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  • 42
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: 1. Isolation and identification of lactic acid bacteria from environmental samples -- 2. Isolation and identification of lactic acid bacteria from environmental samples -- 3. Isolation and identification of lactic acid bacteria from environmental samples -- 4. Isolation and identification of lactic acid bacteria from environmental samples -- 5. Isolation and identification of lactic acid bacteria from environmental samples -- 6. Isolation and identification of lactic acid bacteria from environmental samples -- 7. Isolation and identification of lactic acid bacteria from environmental samples -- 8. Isolation and identification of lactic acid bacteria from environmental samples -- 9. Isolation and identification of lactic acid bacteria from environmental samples -- 10. Isolation and identification of lactic acid bacteria from environmental samples -- 11. Isolation and identification of lactic acid bacteria from environmental samples -- 12. Isolation and identification of lactic acid bacteria from environmental samples -- 13. Isolation and identification of lactic acid bacteria from environmental samples -- 14. Isolation and identification of lactic acid bacteria from environmental samples -- 15. Isolation and identification of lactic acid bacteria from environmental samples -- 16. Isolation and identification of lactic acid bacteria from environmental samples
    Abstract: This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science
    Pages: X, 194 p. 58 illus., 10 illus. in color. : online resource.
    ISBN: 9781493989072
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  • 43
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer -- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood -- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells -- Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo -- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors -- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance -- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity -- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro -- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency -- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity -- Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo -- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments -- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells -- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry -- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways -- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation -- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion -- Quantitative Identification of Senescent Cells in Cancer -- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance -- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment -- Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models -- A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers -- Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy -- Generation of CAR-T Cells for Cancer Immunotherapy
    Abstract: This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field
    Pages: XIII, 363 p. 76 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988853
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    Keywords: Electrophoresis ; Biotechnology ; Electrophoresis ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Fabrication of Glass Microfluidic Devices -- Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices -- Sample Injection Techniques -- Sample Preconcentration Protocols in Microfluidic Electrophoresis -- Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection -- Micellar Electrokinetic Chromatography -- Microchip Isotachophoresis: Analysis of Pharmaceuticals -- Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination -- Nanochannel Gradient Separations -- Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications -- Band Broadening Theories in Capillary Electrophoresis -- Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation -- Microchip Electrophoresis Tools for the Analysis of Small Molecules -- Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out -- Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips -- Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow
    Abstract: This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals
    Pages: X, 255 p. 93 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989645
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  • 45
    Keywords: Plant Ecology ; Anthropology ; Plant anatomy ; Plant physiology ; Plant Systematics/Taxonomy/Biogeography ; Plant Ecology ; Anthropology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Preface -- Overview -- Preparation of qualitative research -- Execution of the qualitative research -- Participant observation and Field Journal -- Audio and video recording techniques for ethnobiological research -- Discourse of the collective subject as a method for analysis of data in ethnobiological research -- Qualitative data analysis -- The meta-analytical and macroecological approach of Ethnobiology -- Collection and analysis of risks associated with environmental changes -- Sensitivity to harvesting and local risk indexes in ethnobotanical research -- The use of multivariate tools in the study of traditional resource management systems -- Multidimensional analysis for testing conservation, ecological and ethnobiological hypotheses -- Ethnoecology in pluricultural contexts -- Ethnobotany and ethnoecology applied to historical ecology -- Biocultural collections -- Accessing the traditional knowledge on the use of cryptogams -- Ethical considerations and protocols in ethnoecological and ethnobiological research -- Challenges in ethnozoological research -- Methodology applied to geographical indication -- Recent advances in research methods and techniques -- Chemical profile and antioxidant activity of natural products.-Collection and extraction of fatty acids from animals and their possible applications in ethnopharmacological research -- Advanced methods in the chemical analysis of medicinal plants -- Population ecology of plant species submitted to extractivism -- Methods for measuring hunting pressure -- Rapid sampling techniques for vertebrate fauna -- Techniques for analyzing the sustainability of hunting -- Description of technological processes for optimization and enhancement of local uses -- Index
    Abstract: Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There have been, however, many advances in research as a result of interaction with different disciplines, but also due to more recent results, new original and interesting questions. This handbook provides the current state of the art methods and techniques in ethnobiology and ethnoecology, and related fields. This new volume, besides bringing new and original aspects of what is found in the literature, fills some of the gaps in volume one by including the most systematic and extensive treatment of methods and techniques in qualitative research. Along with the various methods covered in the individual chapters, the handbook also includes an extensive bibliography that details the current literature in the field
    Pages: XVI, 342 p. 42 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493989195
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  • 46
    Keywords: Biochemistry ; Cell Membranes ; Biochemistry, general ; Membrane Biology ; Biological and Medical Physics, Biophysics ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- 1. Understanding Membranes and Vesicles: A Personal Recollection of the Last Two Decades -- 2. Advanced Concepts and Perspectives of Membrane Physics -- Part 2: Membranes by the Numbers -- 3. Membranes by the Numbers -- Part 3: Spatial Heterogeneities in Biomembranes -- 4. LIPID RAFTS – A PERSONAL ACCOUNT -- 5. Theories of Equilibrium In homogeneous Fluids -- 6. Critical lipidomics: The consequences of lipid miscibility in biological membranes -- 7. Lateral diffusion in heterogeneous cell membranes -- 8. Mechanical factors affecting the mobility of membrane proteins -- 9. Membrane domains under cellular recycling -- 10. Protein Pattern Formation -- Part 4: Biomembrane Mechanics and Consequences for their Functions -- 11. Biomembrane mechanical properties direct diverse cell functions -- 12. Spontaneous and intrinsic curvature of lipid membranes: back to the origins -- 13. Membrane-mediated interactions -- 14. Simulating protein-mediated membrane remodeling at multiple scales -- 15. Mechanosensitivity of membrane budding and tracking Lionel Foret -- 16. Common energetic and mechanical features of membrane fusion and fission machineries -- 17. Interaction of particles and pathogens with biological membranes -- 18. Adhesion of biological membranes -- 19. Spatial and mechanical aspects of signal transduction in the cell membrane -- 20. Protein-Induced morphological deformations of biomembranes -- 21. Fluctuations in active membranes
    Abstract: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike
    Pages: X, 623 p. 167 illus., 144 illus. in color. : online resource.
    ISBN: 9783030006303
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  • 47
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    New York : Springer
    Associated volumes
    Call number: QZ202.6:30(2)
    Keywords: AIDS malignancies ; Viral carcinogenesis ; Oncogenic Viruses ; Tumor Virus Infections ; Neoplasms / etiology ; Acquired Immunodeficiency Syndrome
    Pages: viii, 250 p. : ill. (some col.)
    Edition: 2nd ed.
    ISBN: 9783030035013
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  • 48
    Keywords: Microbiology ; Food science ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products -- Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens -- The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens -- Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection -- Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices -- Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria -- EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes -- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions -- Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation -- Isolation of Bacterial RNA from Foods Inoculated with Pathogens -- The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress -- Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics -- Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells -- High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens -- Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae -- Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection -- Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback -- The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform -- Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics -- Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments -- Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars
    Abstract: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens
    Pages: XI, 278 p. 53 illus., 43 illus. in color. : online resource.
    ISBN: 9781493990009
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  • 49
    Keywords: Agriculture ; Plant breeding ; Plant genetics ; Nutrition ; Agriculture ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Nutrition ; Springer eBooks
    Description / Table of Contents: Preface -- Foreword -- 1. Biofortification of Staple Food crops: An overview -- 2. Marker assisted Breeding for improving the cooking and eating quality of rice -- 3. Improving the nutritional value of potatoes by conventional breeding and genetic modification -- 4. Conventional and molecular breeding approaches for biofortification of pearl millet -- 5. Genetic approaches to improve common bean nutritional quality: current knowledge and future perspectives -- 6. Marker assisted breeding for Beta carotene rich Maize Hybrids -- 7. Recent Advances in Breeding for Modified Fatty Acid Profile in Soybean Oil -- 8. Breeding dry beans with improved cooking and canning quality traits -- 9. Genomic approaches for Biofortification of wheat grain with iron and zinc -- 10. Current Breeding Approaches for Developing Rice with Improved Grain and Nutritional Qualities -- 11. Quality Protein Maize for Nutritional Security -- 12. Genetic improvement for end use quality in wheat -- 13. The use of modern molecular biology and biotechnology tools for improving the Quality value of oilseed Brassicas -- Index
    Abstract: Development of superior crops that have consistent performance in quality and in quantity has not received the same emphasis in the field of genetics and breeding as merited. Specialty trait requires special focus to propagate. Yet basic germplasm and breeding methodologies optimized to improve crops are often applied in the development of improved specialty types. However, because of the standards required for specialty traits, methods of development and improvement are usually more complex than those for common commodity crops. The same standards of performance are desired, but the genetics of the specialty traits often impose breeding criteria distinct from those of non-specialty possessing crops. Specifically, quality improvement programs have unique characteristics that require careful handling and monitoring during their development for specific needs. Adding value either via alternative products from the large volumes of grain produced or development of specialty types is of interest to producers and processors. This work assimilates the most topical results about quality improvement with contemporary plant breeding approaches.The objective of this book is to provide a summary of the germplasm, methods of development, and specific problems involved for quality breeding. In total, fourteen chapters, written by leading scientists involved in crop improvement research, provide comprehensive coverage of the major factors impacting specialty crop improvement
    Pages: XVII, 275 p. 26 illus., 17 illus. in color. : online resource.
    ISBN: 9783030046095
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  • 50
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 51
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    Cham : Springer
    Call number: QZ200:576 ; C100:50
    Pages: vii, 205 p. : ill.
    ISBN: 9783030012069
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    QZ200:576 available
    C100:50 departmental collection or stack – please contact the library
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