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  • Springer eBooks  (3,644)
  • Neurologie
  • Theoretische Physik
  • Messtechnik
  • Strömungsmechanik
  • Springer  (3,574)
  • Springer Singapore  (104)
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  • 1
    Keywords: Biochemistry ; RNA-ligand interactions ; Protein Science ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Predicting Protein-Protein Interactions using SPRINT -- Automated Extraction and Visualization of Protein-Protein Interaction Networks and Beyond: A Text Mining Protocol -- Construction of Functional Protein Networks using Domain Profile Associations -- Reconstruction of Protein-Protein Interaction Networks using Homology-Based Search: Application to the Autophagy Pathway of Ageing in Podospora anserina -- Predicting Interacting Protein Pairs by Co-Evolutionary Paralog Matching -- A Web-Based Protocol for Inter-Protein Contact Prediction by Deep Learning -- Visual Analysis of Protein-Protein Interaction Docking Models using COZOID Tool -- Path-LZerD: Predicting Assembly Order of Multimeric Protein Complexes -- Embedding Alternative Conformations of Proteins in Protein-Protein Interaction Networks -- Informed Use of Protein-Protein Interaction Data: A Focus on the Integrated Interactions Database (IID) -- Generation and Interpretation of Context-Specific Human Protein-Protein Interaction Networks with HIPPIE -- Explore Protein-Protein Interactions for Cancer Target Discovery using the OncoPPi Portal -- Perform Pathway Enrichment Analysis using ReactomeFIViz -- De Novo Pathway Enrichment with KeyPathwayMiner -- De Novo Pathway-Based Classification of Breast Cancer Subtypes -- Vienna Graph Clustering -- On TD-WGcluster - Theoretical Foundations and Guidelines for the User -- An Introductory Guide to Aligning Networks using SANA, the Simulated Annealing Network Aligner
    Abstract: This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: XI, 286 p. 97 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998739
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  • 2
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 3
    Keywords: Cell Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Purinergic Signaling -- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling -- Agonists and Antagonists for Purinergic Receptors -- Homology Modelling of P2X Receptors -- Using RNA Interference for Purinoceptor Knock Down in vivo -- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos -- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues -- Flow Cytometry of Membrane Purinoreceptors -- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation -- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking -- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer -- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy -- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor -- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse -- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging -- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels -- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader -- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes -- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells -- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages -- Automated Planar Patch-clamp Recording of P2X Receptors -- Controlling Engineered P2X Receptors with Light -- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment -- Assays to Measure Purinoceptor Pore Dilation -- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization -- Measuring Leukocyte Migration to Nucleotides -- Assessment of Cell Adhesion after Purinoceptor Activation
    Abstract: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines
    Pages: XII, 360 p. 82 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997176
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  • 4
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels
    Abstract: This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 293 p. 46 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998456
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  • 5
    Keywords: Molecular Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent
    Abstract: This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field
    Pages: X, 300 p. 50 illus., 32 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998821
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  • 6
    Keywords: Human Genetics ; Proteins  ; Human Genetics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies
    Abstract: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field
    Pages: XII, 445 p. 86 illus., 61 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998531
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  • 7
    Keywords: Proteins  ; Biotechnology ; Nanotechnology ; Protein Science ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Introduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins
    Abstract: This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field
    Pages: XIII, 331 p. 84 illus., 64 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998692
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  • 8
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Experimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Abstract: This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study
    Pages: XI, 286 p. 65 illus., 53 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997848
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  • 9
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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  • 10
    Keywords: Infectious Diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Tour de Herpes: Cycling through the Life and Biology of HSV-1 -- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology -- Herpes Simplex Virus Growth, Preparation, and Assay -- Engineering HSV-1 Vectors for Gene Therapy -- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- oHSV Genome Editing by Means of galK Recombineering -- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein -- CRISPR/Cas9-Based Genome Editing of HSV -- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH) -- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing -- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals -- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry -- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells -- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot -- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane -- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination -- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1 -- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
    Abstract: This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV
    Pages: XIV, 457 p. 60 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998142
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  • 11
    Keywords: Neurology ; Neurobiology ; Neurology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Integrative Biomarkers in Stroke -- Vascular-Related Biomarkers of Ischemic Stroke -- Generation and Applicability of Genetic Risk Scores (GRS) in Stroke -- Neuroplasticity Biomarkers in Experimental Stroke Recovery -- Methods of Mitochondrial and Redox Measurements in Ischemic Stroke -- Blood Biomarkers for Stroke Differentiation -- Laser-Capture Micro-Dissection for Measurement of Angiogenesis after Stroke -- Blood-Borne Biomarkers of Hypertension Predicting Hemorrhagic and Ischemic Stroke -- RNA Gene Expression to Identify the Etiology of Acute Ischemic Stroke: The Biomarkers of Acute Stroke Etiology (BASE) Study -- Neurotoxicity Biomarkers Assays Development -- Glutamate Receptor Peptides as Potential Neurovascular Biomarkers of Acute Stroke -- Antibodies to NMDA Receptors in Cerebral and Spinal Cord Infarctions -- Impaired Retinal Vasoreactivity as an Early Marker of Stroke Risk in Diabetes -- Imaging Biomarkers: Keys to Decision Making in Stroke -- Neuroimaging Methods for Acute Stroke Diagnosis and Treatment -- Ultrasound Assessments of Risk for TIA and Stroke in Vascular Surgery -- Preoperative and Intraoperative Markers of Cerebral Ischemia -- Time is Brain: the Prehospital Phase and the Mobile Stroke Unit -- Acute Clinical Intervention and Chronic Management of Cerebral Vascular Accident -- Trends in Biomarkers Development for Stroke
    Abstract: This volume presents the latest data on recent achievements in new and emerging technologies for stroke biomarkers and innovations in stroke assessment. The topics discussed in this book explore the role of upcoming biomarkers in different types of stroke, and explores techniques that will allow researchers to be more effective when approaching clinical management and patient care. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to ensure successful results in the laboratory. Cutting-edge and comprehensive, Stroke Biomarkers is a valuable resource for both experimental and clinical scientists interested in expanding their knowledge in the field of stroke research
    Pages: XVII, 425 p. 80 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493996827
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  • 12
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
    Abstract: The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
    Pages: XI, 247 p. 30 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998654
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  • 13
    Keywords: Bacteriology ; Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing of Staphylococcus aureus -- Genetic Manipulations of Staphylococcal Chromosomal DNA -- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology -- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus -- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus -- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology -- Metabolomic Profiling of Staphylococcus aureus -- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells -- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging -- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options -- Animal Models for Drug Development for MRSA
    Abstract: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens
    Pages: XI, 268 p. 35 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998494
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  • 14
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook -- Immunophenotyping by Mass Cytometry -- Fluorescent Cell Barcoding for Immunophenotyping -- Immunophenotyping using Dried and Lyophilized Reagents -- Guidelines for Gating Flow Cytometry Data for Immunological Assays -- Strategies and Techniques for NK Cell Phenotyping -- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue -- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells -- Immunophenotyping of Human Regulatory T Cells -- Immunophenotyping of Human Innate Lymphoid Cells -- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis -- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles -- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells -- Quality Control of Immunophenotyping -- Immunophenotyping of Acute Myeloid Leukemia -- Immunophenotyping of Acute Lymphoblastic Leukemia -- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia -- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
    Abstract: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field
    Pages: X, 356 p. 94 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493996506
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  • 15
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures
    Abstract: This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research
    Pages: XV, 363 p. 72 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493976171
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  • 16
    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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  • 17
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Anesthesiology ; Neurosciences ; Anesthesiology ; Springer eBooks
    Description / Table of Contents: The Challenge of Accidental Awareness during General Anesthesia -- Impact of Anesthetics on Brain Electrical Activity and Principles of pEEG-Based Monitoring during General Anethesia -- Perioperative Monitoring of Autonomic Nervous Activity -- Monitoring Cerebral Oximetry by Near Infrared Spectroscopy (NIRS) in Anaesthesia and Critical Care: Progress and Perspectives -- Post-Traumatic Stress Disorder following Intraoperative Awareness -- Mechanisms of Action of Inhaled Volatile General Anesthetics: Unconsciousness at the Molecular Level -- Intravenous Hypnotic Agents: From Binding Sites to Loss of Consciousness -- Pharmacological Considerations for the Use of General Anesthetics in the Elderly -- Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies -- Postoperative Cognitive Function following General Anesthesia in Children -- The Challenge of Opioid-Free Anesthesia -- New Insights into the Pharmacology of Dexmedetomidine and Open Issues for Neurosurgical Procedures -- Transgenic Mouse Models, General Anesthetics and Alzheimer Disease: Findings from Preclinical Studies -- The Biochemical Basis of Delirium -- Postoperative Delirium and Postoperative Cognitive Dysfunction
    Abstract: This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology. Furthermore, chapters in this book cover technical topics such as preoperative monitoring of autonomic nervous activity; EEG based monitoring of general anesthesia; monitoring cerebral oximetry by near infrared spectroscopy; pharmacological issues of anesthesia interest; and optimization of perioperative course through the latest techniques such as the opioid-free anesthesia. Finally, this book offers a comprehensive and up-to-date discussion on the phenomenon of general anesthesia awareness and its potential psychological sequelae. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, General Anesthesia Research is a valuable resource for novice and expert anesthesiologists who want to learn more about the important advances made in this developing field
    Pages: XIV, 256 p. 15 illus., 14 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998913
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  • 18
    Keywords: Mass spectrometry ; Proteomics ; Mass spectrometry ; Proteomics ; Springer eBooks
    Description / Table of Contents: Introduction to Mass Spectrometry-based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Comparing Peptide Spectra Matches Across Search Engines -- Calculation of False Discovery Rate for Peptide and Protein Identification -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Interpretation of Tandem Mass Spectra of Posttranslationally Modified Peptides -- Solution to Dark Matter Identified by Mass-tolerant Database Search -- Phosphoproteomics Profiling to Identify Altered Signaling Pathways and Kinase-targeted Cancer Therapies -- Mass Spectrometry Based Characterization of Ub- and UbL-modified Proteins -- Targeted Proteomics as a Tool for Quantifying Urine-based Biomarkers -- Data Imputation in Merged Isobaric Labelling-based Relative Quantification Datasets -- Clustering Clinical Data in R -- Review of Issues and Solutions to Data Analysis Reproducibility and Data Quality in Clinical Proteomics -- Review of Batch Effects Prevention, Diagnostics, and Correction Approaches -- Using the Object-oriented PowerShell for Simple Proteomics Data Analysis -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data
    Abstract: The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics. In contrast to the previous editions, this third edition aims to provide the most relevant computational methods, focusing on computational concepts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Data Analysis in Proteomics, Third Edition to ensure successful results in the further study of this vital field
    Pages: X, 445 p. 136 illus., 94 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493997442
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  • 19
    Keywords: Neurobiology ; Developmental Biology ; Neurosciences ; Laboratory animals ; Neurobiology ; Developmental Biology ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra -- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis -- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis -- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System -- Immunostaining of the Embryonic and Larval Drosophila Brain -- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila -- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling -- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update -- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain -- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila -- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development -- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum -- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium -- X-Ray Microscopy of the Larval Crustacean Brain -- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain -- Immunostainings in Nervous System Development of the Nematode C. elegans -- Methods in Brain Development of Molluscs -- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization -- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum -- In Situ Hybridization and Immunostaining of Xenopus Brain -- Morpholino Studies in Xenopus Brain Development -- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish -- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish -- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain -- Gene Silencing in Chicken Brain Development -- Transplantation of Neural Tissue: Quail-Chick Chimeras -- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development -- The Cre/Lox System to Assess the Development of the Mouse Brain -- In Utero Electroporation to Study Mouse Brain Development
    Abstract: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain
    Pages: XIV, 527 p. 116 illus., 96 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493997329
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  • 20
    Keywords: Microbial genetics ; Microbial genomics ; Bacteriology ; Microbial Genetics and Genomics ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples -- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health -- Detection, Isolation, and Characterization of Plasmids in the Environment -- Bacteriophage Isolation and Characterization: Phages of Escherichia coli -- Detection and Characterization of Transposons in Bacteria -- Measuring Plasmid Conjugation Using Antibiotic Selection -- Measuring Plasmid Conjugation Using Fluorescent Reporters -- Methods to Quantify DNA Transfer in Enterococcus -- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis -- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases -- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis -- First Biochemical Steps on Bacterial Transposition Pathways -- Natural Transformation in Escherichia coli -- Integron Identification in Bacterial Genomes and Cassette Recombination Assays -- Methods to Identify and Analyze Vesicle-Protected DNA Transfer -- Measuring Plasmid Stability in Gram-Negative Bacteria -- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems -- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements -- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan -- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS) -- MOBscan: Automated Annotation of MOB Relaxases -- Plasmid Typing and Classification -- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets -- Statistical Analysis of Accessory Genome -- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits -- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria -- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria -- Conjugative Assembly Genome Engineering (CAGE)
    Abstract: This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality
    Pages: XV, 413 p. 62 illus., 52 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998777
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  • 21
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example
    Abstract: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field
    Pages: XIV, 405 p. 76 illus., 66 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998180
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  • 22
    Keywords: Antibodies ; Pharmacology ; Antibodies ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Overview of the Current ADC Discovery Landscape -- Pushing the Envelope: Advancement of ADCs Outside of Oncology -- Conjugations to Endogenous Cysteine Residues -- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies -- Transglutaminase-Mediated Conjugations -- Click Chemistry Conjugations -- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates -- Bridged Cysteine Conjugations -- Antibody Conjugations via Glycosyl Remodeling -- ADC Analysis by Hydrophobic Interaction Chromatography -- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs -- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction -- Analysis of ADCs by Native Mass Spectrometry -- High Resolution Characterization of ADCs by Orbitrap LCMS -- Conjugation Site Analysis by MS/MS Protein Sequencing -- Conjugation Site Analysis of Lysine-Conjugated ADCs -- Characterization of ADCs by Capillary Electrophoresis -- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry -- Purification of ADCs by Hydrophobic Interaction Chromatography -- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations -- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions -- Biophysical Methods for Characterization of Antibody Drug Conjugates -- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines -- LC/MS Methods for Studying Lysosomal ADC Catabolism -- Assessing ADC Plasma Stability by LC/MS Methods -- Determination of ADC Concentration by Ligand-Binding Assays
    Abstract: This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the €œáctivation barrieŕ€ when undertaking a new discipline, and provides a ́€œtoolbox́€ for the next generation of ADC scientists
    Pages: XI, 373 p. 138 illus., 109 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999293
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  • 23
    Keywords: Biomedical Engineering ; Cell Biology ; Bioinformatics ; Biology—Technique ; Systems Biology ; Biological systems ; Biomedical Engineering/Biotechnology ; Cell Biology ; Computational Biology/Bioinformatics ; Biological Techniques ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB: Image Analysis & Brownian Motion -- Resolving the process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM)
    Abstract: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images
    Pages: X, 170 p. 69 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030223861
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  • 24
    Keywords: Immunology ; Medical virology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus -- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV -- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR) -- Establishment of Replication-competent HCV Strain with Minimum Modifications -- Full-length Open Reading Frame Amplification of Hepatitis C Virus -- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing -- A Cell Culture Model for Persistent HCV Infection -- Expression Cloning of Host Factors Required for the HCV Replication Cycle -- Application of Deep Mutational Scanning in Hepatitis C Virus -- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals -- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay -- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells -- Functional Characterization of Apolipoproteins in the HCV Life Cycle -- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes -- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins -- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis -- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells -- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact -- Complement Regulation and Immune Evasion by Hepatitis C Virus -- Direct ex vivo Functional Analysis of HCV-specific T Cells -- ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry -- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display -- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA -- In vitro Neutralization Assay using Cultured Hepatitis C Virus -- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp) -- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice -- Mice with Chimeric Human Livers and Their Applications -- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies
    Abstract: This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIX, 508 p. 75 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989768
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  • 25
    Keywords: Biological models ; Science / Philosophy ; Endangered ecosystems ; Botany ; Systems Biology ; Philosophy of Science ; Ecosystems ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- The Basics -- Is there anything new under the sun? -- Instability as the core of emergence -- Emergence and modularity in life sciences and beyond -- The emergence of sustainability -- From modules to emergent holistic properties in living organisms -- Modularity versus emergence: How to cope with complexity in whole plant physiology? -- Emergence in biomimetic materials systems -- Roots of complexity in the self-referential genetic code -- Brains emerging: on modularity and self-organization of neural development in vivo and in vitro -- Plants: Unitary organisms emerging from integration and self-organization of modules -- Ecology: Ecosystems and biodiversity -- Ultimate Integration -- Emergent properties and stability in hierarchical bio-systems: there is no privileged level of causation -- Emergence and sustainment of humankind on Earth: the categorical imperative -- Gaia – a holobiont-like system emerging from interaction
    Abstract: This book focuses on modules and emergence with self-organization in the life sciences. As Aristotle observed so long ago, the whole is more than the sum of its parts. However, contemporary science is dominated by reductionist concepts and tends to neglect the non-reproducible features of complex systems, which emerge from the interaction of the smaller units they are composed of. The book is divided into three major parts; the essays in part A highlight the conceptual basis of emergence, linking it to the philosophy of science, systems biology and sustainability. This is subsequently exemplified in part B by applying the concept of emergence to various biological disciplines, such as genetics, developmental biology, neurobiology, plant physiology and ecology. New aspects of emergence come into play when biology meets the technical sciences, as revealed in a chapter on bionics. In turn, part C adopts a broader view, revealing how the organization of life follows a hierarchical order in terms of scalar dimensions, ranging from the molecular level to the entire biosphere. The idea that life is primarily and exclusively shaped by processes at the molecular level (and, in particular, by the information encoded in the genome) is refuted; rather, there is no hierarchy with respect to the level of causation in the cross-talk between the levels. In the last two chapters, the evolutionary trend toward ever-increasing complexity in living systems is interpreted in terms of the Gaia hypothesis sensu Lovelock: the entire biosphere is viewed as a functional unit (or ‘holobiont-like system’) organized to develop and sustain life on Earth
    Pages: XIV, 279 p. 62 illus., 46 illus. in color. : online resource.
    ISBN: 9783030061289
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  • 26
    Keywords: Immunology ; Emerging infectious diseases ; Immunology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: 1. Epidemiology of extrapulmonary tuberculosis -- 2. Pleural Tuberculosis -- 3. Gastrointestinal Tuberculosis -- 4. Hepatobiliary and splenic tuberculosis -- 5. Lymphatic Tuberculosis -- 6. Bone and Joint Tuberculosis -- 7. Tuberculous Spondylodiscitis -- 8. Tuberculous Meningitis -- 9. Tuberculous Encephalitis -- 10. Spinal tuberculosis -- 11. Genitourinary Tuberculosis -- 12. Cardiovascular Tuberculosis -- 13. Cutaneous Tuberculosis -- 14. Tuberculosis of Eye -- 15. Infection control in extrapulmonary TB -- 16. Tuberculosis in the ICU
    Abstract: This book brings awareness to a neglected condition that is nevertheless prevalent world-wide. Much focus is justly given to pulmonary tuberculosis, one of the key medical scourges of humanity, but this disease also often manifests itself in organs outside of the lungs. There is however a surprising lack of information available on extrapulmonary TB, which this book aims to remedy. Specifically, research, as well as epidemiology, diagnosis, and treatment options, are discussed in detail by an international list of experts. This comprehensive product serves as a valuable resource to numerous fields of medicine due to the presence of extrapulmonary tuberculosis throughout the human body
    Pages: VIII, 208 p. 7 illus., 1 illus. in color. : online resource.
    ISBN: 9783030047443
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  • 27
    Keywords: Microbiology ; Biomedical Engineering ; Microbial ecology ; Microbiology ; Biomedical Engineering/Biotechnology ; Microbial ecology ; Applied Microbiology ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: Chapter 1. The Dawn of Novel Biotechnological Applications of Polyhydroxyalkanoates -- Chapter 2. Strategy for biosynthesis of polyhydroxyalkonates polymers/copolymers and their application in drug delivery -- Chapter 3. Applications of Polyhydroxyalkanoates and its metabolites as transporters for bioactive molecules -- Chapter 4. Polyhydroxyalkanoates applications in antimicrobial agents' delivery and wound healing -- Chapter 5. Polyhydroxyalkanoates applications in Drug Carriers -- Chapter 6. Applications of polyhydroxyalkanoate based nanovehicles -- Chapter 7. Memory enhancers -- Chapter 8. Novel Biocontrol Agents: Short Chain Fatty Acids And More Recently, Polyhydroxyalkanoates -- Chapter 9. Potential Therapeutic Applications of Polyhydroalkanoates and Their Composites as Anti-microbial Agents -- Chapter 10. Polyhydroxyalkanoates (PHA) – Applications in Wound Treatment and as Precursors for Oral Drugs -- Chapter 11. Exploiting Polyhydroxyalkanoates for Tissue Engineering -- Chapter 12. CH4-based polyhydroxyalkanoate production: a step further towards a sustainable bioeconomy -- Chapter 13. Applications of PHA in Agriculture -- Chapter 14. Polyhydroxyalkanoates in Packaging -- Chapter 15. Approaches for Enhancing Extraction of bacterial polyhydroxyalkanoates for Industrial Applications -- Chapter 16. Nanofibers from polyhydroxyalkanoates and their applications in tissue engineering
    Abstract: This book presents the latest research on the uses of polyhydroxyalkanoates (PHA), introducing readers to these natural, biodegradable polyesters produced by microorganisms, their functions and applications. The individual chapters discuss the various potentials of these bioplastics, which offer an attractive alternative to non-biodegradable plastics. The book also describes the diverse medical and biomedical applications of PHAs, including their use as drug carriers, memory enhancers, and biocontrol agents, and examines their role in creating a more sustainable economy – which is the need of the hour
    Pages: XII, 420 p. 42 illus., 34 illus. in color. : online resource.
    ISBN: 9789811337598
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  • 28
    Keywords: Biomedical Engineering ; Neurosciences ; Radiology, Medical ; Neurology ; Biomedical Engineering/Biotechnology ; Neurosciences ; Diagnostic Radiology ; Mathematical and Computational Biology ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Introduction -- Chapter 2. Multifractal study of EEG signal of subjects with Epilepsy and Alzheimer’s -- Chapter 3. Multifractal Approach for Quantification of Autonomic Deregulation due to Epileptic Seizure with ECG data -- Chapter 4. Multifractal Analysis of Electromyography data.-Chapter 5. Multifractal study on Parkinson’s and Huntington diseases with human gait data -- Chapter 6. Multifractal correlation study between posture and autonomic deregulation using ECG and blood pressure data -- Chapter 7. Appendix
    Abstract: This book primarily focuses on the study of various neurological disorders, including Parkinson’s (PD), Huntington (HD), Epilepsy, Alzheimer’s and Motor Neuron Diseases (MND) from a new perspective by analyzing the physiological signals associated with them using non-linear dynamics. The development of nonlinear methods has significantly helped to study complex nonlinear systems in detail by providing accurate and reliable information. The book provides a brief introduction to the central nervous system and its various disorders, their effects on health and quality of life, and their respective courses of treatment, followed by different bioelectrical signals like those detected by Electroencephalography (EEG), Electrocardiography (ECG), and Electromyography (EMG). In turn, the book discusses a range of nonlinear techniques, fractals, multifractals, and Higuchi’s Fractal Dimension (HFD), with mathematical examples and procedures. A review of studies conducted to date on neurological disorders like epilepsy, dementia, Parkinson’s, Huntington, Alzheimer’s, and Motor Neuron Diseases, which incorporate linear and nonlinear techniques, is also provided. The book subsequently presents new findings on neurological disorders of the central nervous system, namely Parkinson’s disease and Huntington’s disease, by analyzing their gait characteristics using a nonlinear fractal based technique: Multifractal Detrended Fluctuation Analysis (MFDFA). In closing, the book elaborates on several parameters that can be obtained from cross-correlation studies of ECG and blood pressure, and can be used as markers for neurological disorders
    Pages: XX, 182 p. 77 illus., 51 illus. in color. : online resource.
    ISBN: 9789811335525
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  • 29
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- A Neuron-Glial perspective for computational neuroscience -- Part 2. Calcium dynamics -- Data-driven modelling of the inositol trisphosphate receptor and its role in calcium induced calcium release -- Intracellular calcium dynamics: biophysical and simplified models -- Modeling of stochastic Ca2+ signals -- G protein-coupled receptor-mediated astrocytic Ca2+ signaling in astrocytes -- Emergence of regular and complex calcium oscillations by inositol 1,4,5-trisphosphate signaling in astrocytes -- Astrocyte networks and intercellular calcium propagation -- Part 3. Tripartite Synapse and regulation of network activity -- Tripartite synapses and regulation of synaptic plasticity -- Purinergic signaling at tripartite synapses -- Gliotransmitter exocytosis and its consequences on synaptic transmission -- Computational models of pathophysiological glial activation in CNS disorders -- Part 4. Homeostasis and Metabolic coupling -- The role of astrocytes in neurotransmitter uptake and brain metabolism -- Glutamate uptake -- Astrocytic ion dynamics: Implications for potassium buffering and liquid flow -- Constraint-Based Modeling of Metabolic Interactions in and between Astrocytes and Neurons -- Part 5. Computational Tools to Analyze and Model Astrocyte Experiments -- Computational models of astrocytes and astrocyte-neuron interactions: Characterization, reproducibility, and future perspectives -- GECIquant: semi-automated detection and quantification of astrocyte intracellular Ca2+ signals monitored with GCaMP6f -- Modeling neuron-glia interactions with the Brian 2.0 simulator
    Abstract: Over the last two decades, the recognition that astrocytes - the predominant type of cortical glial cells - could sense neighboring neuronal activity and release neuroactive agents, has been instrumental in the uncovering of many roles that these cells could play in brain processing and the storage of information. These findings initiated a conceptual revolution that leads to rethinking how brain communication works since they imply that information travels and is processed not just in the neuronal circuitry but in an expanded neuron-glial network. On the other hand the physiological need for astrocyte signaling in brain information processing and the modes of action of these cells in computational tasks remain largely undefined. This is due, to a large extent, both to the lack of conclusive experimental evidence, and to a substantial lack of a theoretical framework to address modeling and characterization of the many possible astrocyte functions. This book that we propose aims at filling this gap, providing the first systematic computational approach to the complex, wide subject of neuron-glia interactions. The organization of the book is unique insofar as it considers a selection of ́€œhot topicś€ in glia research that ideally brings together both the novelty of the recent experimental findings in the field and the modelling challenge that they bear. A chapter written by experimentalists, possibly in collaboration with theoreticians, will introduce each topic. The aim of this chapter, that we foresee less technical in its style than in conventional reviews, will be to provide a review as clear as possible, of what is €œéstablished́€ and what remains speculative (i.e. the open questions). Each topic will then be presented in its possible different aspects, by 2-3 chapters by theoreticians. These chapters will be edited in order to provide a ́€œpriminǵ€ reference for modeling neuron-glia interactions, suitable both for the graduate student and the professional researcher
    Pages: XV, 505 p. 205 illus., 76 illus. in color. : online resource.
    ISBN: 9783030008178
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  • 30
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: I-Cell Disease (Mucolipidosis II): A Case Series from a Tertiary Paediatric Centre Reviewing the Airway and Respiratory Consequences of the Disease -- Oral Ganglioside Supplement Improves Growth and Development in Patients with Ganglioside GM3 Synthase Deficiency -- Feeding Difficulties and Orofacial Myofunctional Disorder in Patients with Hepatic Glycogen Storage Diseases -- Auxiliary Partial Orthotopic Liver Transplantation for Monogenic Metabolic Liver Diseases: Single-Centre Experience -- A Novel Truncating FLAD1 Variant, Causing Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) in an 8-Year-Old Boy -- The Effect of Continuous Intravenous Glucagon on Glucose Requirements in Infants with Congenital Hyperinsulinism -- Case of Neonatal Fatality from Neuromuscular Variant of Glycogen Storage Disease Type IV -- Acute and Chronic Management in an Atypical Case of Ethylmalonic Encephalopathy -- Dihydropyrimidine Dehydrogenase Deficiency: Homozygosity for an Extremely Rare Variant in DPYD due to Uniparental Isodisomy of Chromosome 1 -- Severe Neonatal Manifestations of Infantile Liver Failure Syndrome Type 1 Caused by Cytosolic Leucine-tRNA Synthetase Deficiency -- Enzyme Replacement Therapy in Pregnant Women with Fabry Disease: A Case Series -- Survival of a Male Infant with a Familial Xp11.4 Deletion Causing Ornithine Transcarbamylase Deficiency -- The Unique Spectrum of Mutations in Patients with Hereditary Tyrosinemia Type 1 in Different Regions of the Russian Federation -- Elevated Lyso-Gb3 Suggests the R118C GLA Mutation Is a Pathological Fabry Variant -- Glycogen Storage Disease Type IV: A Rare Cause for Neuromuscular Disorders or Often Missed? -- A Hemizygous Deletion Within the PGK1 Gene in Males with PGK1 Deficiency
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 110 p. 28 illus., 13 illus. in color. : online resource.
    ISBN: 9783662586471
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  • 31
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Plant genetics ; Bacteriology ; Bioinformatics ; Human Genetics ; Microbial Genetics and Genomics ; Microbial ecology ; Plant Genetics and Genomics ; Bacteriology ; Bioinformatics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part 1. Concepts and Approaches -- Chapter1. Computational Methods in Microbial Population Genomics -- Chapter2. What Microbial Population Genomics has taught us about Speciation -- Chapter3. Peering into the genetic makeup of natural microbial populations using metagenomics -- Chapter4. A reverse ecology framework for bacteria and archaea -- Part2. Population genomics of bacteria and archaea -- Chapter5. What is a Pseudomonas syringae Population? -- Chapter6. An introductory narrative to the population genomics of pathogenic bacteria, exemplified by Neisseria meningitidis -- Chapter7. Population genomics of archaea: Signatures of archaeal biology from natural populations -- Part3. Population genomics of fungi -- Chapter8. Advances in Genomics of Human Fungal Pathogens -- Chapter9. Yeast population genomics goes wild: The case of Saccharomyces paradoxus -- Part4. Population genomics of viruses -- Chapter10. Population genomics of plant viruses -- Chapter11. Population genomics of human viruses -- Chapter12. Population genomics of bacteriophages
    Abstract: Population genomics is a rapidly emerging field that has the potential to transform our understanding of how evolutionary forces shape genomic diversity among microbes. There have already been considerable advances in understanding gene flow and spread of adaptive traits, and in linking epidemiology with evolutionary biology. The current challenge is to find unifying evolutionary principles for organisms that display a wide range of reproductive biology – from highly clonal to promiscuous – and for which the vast majority have eluded cultivation. This requires interdisciplinary approaches that incorporate novel computational tools, testing of existing and novel population genetic models, and creative new ways of linking genetic diversity to ecological factors. This pioneering book will discuss the advances made and promises of population genomics in microorganisms, outlining some of the key theoretical and practical challenges for microbial population genomics, including defining and identifying populations, genomics-based reverse ecology and building appropriate tools to understand microbes in a variety of complex environments
    Pages: XIII, 344 p. : online resource.
    ISBN: 9783030047566
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  • 32
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: A Homozygous Splice Site Mutation in SLC25A42, Encoding the Mitochondrial Transporter of Coenzyme A, Causes Metabolic Crises and Epileptic Encephalopathy -- Apparent Acetaminophen Toxicity in a Patient with Transaldolase Deficiency -- Sialuria: Ninth Patient Described Has a Novel Mutation in GNE -- Stability of the ABCD1 Protein with a Missense Mutation: A Novel Approach to Finding Therapeutic Compounds for X-Linked Adrenoleukodystrophy -- Psychosocial Functioning in Parents of MPS III Patients -- The Second Case of Saposin A Deficiency and Altered Autophagy -- An Electronic Questionnaire for Liver Assessment in Congenital Disorders of Glycosylation (LeQCDG): A Patient-Centered Study -- Demographics, Clinical Features, and Mortality of Acute Hepatic Porphyrias in Colombia: An Analysis of 101 Patients -- Cobalamin D Deficiency Identified Through Newborn Screening -- Lathosterolosis: A Relatively Mild Case with Cataracts and Learning Difficulties -- DPAGT1 Deficiency with Encephalopathy (DPAGT1-CDG): Clinical and Genetic Description of 11 New Patients -- Enzyme Replacement Therapy During Pregnancy in Fabry Patients -- Hyperornithinemia, Hyperammonemia, and Homocitrullinuria Syndrome Causing Severe Neonatal Hyperammonemia -- Screening for Niemann-Pick Type C Disease in a Memory Clinic Cohort -- Reversible Cerebral White Matter Abnormalities in Homocystinuria
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 119 p. 32 illus., 22 illus. in color. : online resource.
    ISBN: 9783662586174
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  • 33
    Keywords: Emerging infectious diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Analysis of Iron Requirements and Siderophore Production -- The Pathometabolism of Legionella Studied by Isotopologue Profiling -- Quantification of Viable but Non-culturable Cells of Legionella pneumophila -- Selection of Legionella Virulence-related Traits by Environmental Protozoa -- Migration of Acanthamoeba castellanii through Legionella Biofilms -- Scar-free Genome Editing in Legionella pneumophila -- Querying Legionella Genomes using Transposition-sequencing -- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq -- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae -- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium -- Sorting of Phagocytic Cells Infected with Legionella pneumophila -- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment -- Mitochondrial Dynamics and Activity in Legionella-Infected Cells -- Perturbation of Legionella Cell Infection by RNA Interference -- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila -- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography -- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila -- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification -- Inflammasome Activation in Legionella-infected Macrophages -- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila -- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence -- Dictyostelium Host Response to Legionella Infection: Strategies and Assays -- 6.1.3 The Caenorhabditis elegans model of Legionella infection -- The Mouse as a Model for Pulmonary Legionella Infection -- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples -- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients -- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry
    Abstract: The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens
    Pages: XXVI, 476 p. 74 illus., 50 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990481
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  • 34
    Keywords: Biochemistry ; Medical laboratories ; Protein Science ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Using Data Mining to Explore Calmodulin Bibliography -- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator -- High Resolution Calcium Imaging Method for Local Calcium Signaling -- Measurement of Contractility and Calcium Release in Cardiac Spheroids -- Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis -- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio) -- Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements -- Designing Calcium Binding Proteins for Molecular MR Imaging -- Coordination to Divalent Cations by Calcium Binding Proteins -- Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo -- Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets -- Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes -- Expression and Purification of Calmodulin for NMR and Other Biophysical Applications -- The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells -- Calpain Purification through Calpastatin and Calcium: Strategy and Procedures -- Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins -- Expression and Characterization of MICU2, a Ca2+ Sensor Protein -- S100 Proteins in the Innate Immune Response to Pathogens -- Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors -- Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy -- Isolation and Characterization of S100 Protein-Protein Complexes -- The Multifaceted S100A4 Protein in Cancer and Inflammation -- Interaction of S100A6 with Target Proteins In Vitro and in Living Cells -- Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7) -- Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin -- Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography -- Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex -- Analysis of S100A11 in DNA Damage Repair -- Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography -- Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure -- High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins -- Calcium-Induced Protein Folding in Calumenin and Calmodulin -- Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing -- Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies -- Tryptophan Scanning Mutagenesis of EF-Hand Motifs -- Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling -- Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level -- Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation -- Characterization of Calcium-Binding Proteins from Parasitic Worms -- High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome -- Targeted Mass Spectrometry of S100 Proteins -- Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury -- Serum S100B Levels in Melanoma -- The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine -- S100A7 in Psoriasis: Immunodetection and Activation by CRISPR -- S100A8/A9 in Myocardial Infarction -- Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults -- S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions
    Abstract: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry
    Pages: XIX, 779 p. 175 illus., 117 illus. in color. : online resource.
    ISBN: 9781493990306
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  • 35
    Keywords: Neurosciences ; Stem Cells ; Laboratory animals ; Neurosciences ; Stem Cells ; Animal Models ; Springer eBooks
    Description / Table of Contents: Generation of Neural Stem Cells from Induced Pluripotent Stem Cells -- Making NSC and Neurons from Patient Derived Tissue Samples -- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury -- Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells -- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells -- In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology -- Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons -- Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene -- Single-Cell Library Preparation of iPSC-derived Neural Stem Cells -- Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells -- Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons -- Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach -- Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells -- Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation -- Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart -- Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion
    Abstract: This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIV, 239 p. 51 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990078
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  • 36
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 37
    Keywords: Bioinformatics ; Human Genetics ; Bioinformatics ; Human Genetics ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: ncRNAs in Inflammatory and Infectious Diseases -- p73-Governed miRNA Networks: Translating Bioinformatics Approaches to Therapeutic Solutions for Cancer Metastasis -- Methods for Annotation and Validation of Circular RNAs from RNAseq Data -- Methods to Study Long Non-coding RNA Expression and Dynamics in Zebrafish using RNA Sequencing -- Workflow Development for the Functional Characterization of ncRNAs -- ncRNA Editing: Functional Characterization and Computational Resources -- Computational Prediction of Functional microRNA – mRNA Interactions -- Tools for Understanding miRNA-mRNA Interactions for Reproducible RNA Analysis -- Computational Resources for Prediction and Analysis of Functional miRNA and their Targetome -- Non-coding RNAs Databases: Current Status and Trends -- Controllability Methods for Identifying Associations between Critical Control ncRNAs and Human Diseases -- Network-based Methods and Other Approaches for Predicting lncRNA Functions and Disease Associations -- Integration of miRNA and mRNA Expression Data for Understanding Etiology of Gynecologic Cancers -- Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks -- Kinetic Modeling of Competition and Depletion of Shared miRNAs by Competing Endogenous RNAs -- Modeling ncRNA-mediated Circuits in Cell Fate Decision -- Modeling Long ncRNA-mediated Regulation in the Mammalian Cell Cycle
    Abstract: This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field
    Pages: XII, 451 p. 78 illus., 65 illus. in color. : online resource.
    ISBN: 9781493989829
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  • 38
    Keywords: Pharmaceutical technology ; Chromatography ; Chemical engineering ; Pharmaceutical Sciences/Technology ; Chromatography ; Industrial Chemistry/Chemical Engineering ; Springer eBooks
    Description / Table of Contents: Separation and Purification of Amino acids -- Ion exchange chromatography for enzyme immobilization -- Determination of morphine in urine -- Chromatographic Separation of Amino Acids -- Applications of ion exchange chromatography in pharmaceutical analysis -- Thermodynamic Kinetics and Sorption of Bovine Serum Albumin with Different Clay materials -- Sorbitol demineralization by ion exchange -- Separation and purification of nucleotides, nucleosides, purine and pyrimidine bases by ion exchange -- Separation and purification of vitamins: vitamin B1, B2, B6, C, and K1 -- Colour removal from sugar syrups
    Abstract: This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists
    Pages: XII, 225 p. 41 illus., 29 illus. in color. : online resource.
    ISBN: 9783030060824
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  • 39
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single Molecule FRET Methods to Study Glutamate Receptors -- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) -- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy -- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors -- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function -- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) -- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus -- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats -- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction -- Gene Expression Analysis by Multiplex Single Cell RT-PCR -- Metabolomics Analysis of Glutamate Receptor Function -- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation -- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function -- Fractionation of Subcellular Compartments from Human Brain Tissue -- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
    Abstract: This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications
    Pages: X, 260 p. 67 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990771
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  • 40
    Keywords: Embryology ; Laboratory animals ; Animal genetics ; Embryology ; Animal Models ; Animal Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments -- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos -- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos -- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts -- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis -- Embryological and Genetic Manipulation of Chick Development -- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos -- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens -- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) -- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos -- Ex Utero Culture and Imaging of Mouse Embryos -- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections -- Reptile Embryology and Regeneration -- Manipulation of Developmental Function in Turtles with Notes on Alligators -- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes -- Visualizing the Balbiani Body in Zebrafish Oocytes -- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis -- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos -- Germ Cell Transplantation in Avian Species -- Production of Germ-Line Chimeras in Zebrafish -- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring -- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) -- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish -- Modeling Embryonic Cleavage Patterns -- Laboratory Guidelines for Animal Care
    Abstract: This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development. Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
    Pages: XI, 434 p. 82 illus., 70 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990092
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  • 41
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 42
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 43
    Keywords: Medicine ; Dentistry ; Molecular Medicine ; Dentistry ; Springer eBooks
    Description / Table of Contents: Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors -- Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line -- Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells -- Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors -- Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis -- Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions -- Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering -- Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry -- Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering -- In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy -- A Mouse Model to Study Reparative Dentinogenesis -- Multiwalled Carbon Nanotubes for Dental Applications -- Peptide-mediated Biomimetic Regrowth of Human Enamel in situ -- Bioengineering Tooth Bud Constructs using GelMA Hydrogel -- Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes -- In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes -- Methods for in situ Proteins Visualization in Dental Mineralized Tissues -- In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection -- Immunofluorescence Procedure for Developing Enamel Tissues -- Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos -- Isolation of SIBLING Proteins from Bone and Dentin Matrix -- Immunohistochemical Co-localization of Amelogenin and Ameloblastin in Developing Enamel Matrix -- The Expression and Purification of Recombinant Mouse Ameloblastin in e-coli -- Protocols for Studying Formation and Mineralization of Dental Tissues in vivo -- Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth -- Purification of Developing Enamel Matrix Proteins using Preparative SDS PAGE -- Using ImageJ (FIJI) to Analyse and Present X Ray CT Images of Enamel -- Scanning Electron Microscopy (SEM) Methods for Dental Enamel -- Microcomputed Tomography Imaging in Odontogenesis Studies -- Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues -- Rodent Dental Fluorosis Model--Extraction of Enamel Organ from Rat Incisors -- Three Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-beam Computed Tomography (CBCT) -- Protocols to Study Dental Caries In Vitro: Microbial Caries Models -- In vitro Caries Models for the Assessment of Novel Restorative Materials -- Protocols to Study Dental Caries In Vitro: pH Cycling Models -- In Vivo Rodent Models for Studying Dental Caries and Pulp Disease -- Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement -- Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues -- Protocols, Methods, and Tools for Genome-wide Association Studies (GWAS) of Dental Traits -- Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition -- The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Oral Metagenomics, Metatranscriptomics and Metabolomics Studies of the Oral Microbiome -- Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarkers Screening
    Abstract: This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva, and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth
    Pages: XVIII, 565 p. 152 illus., 135 illus. in color. : online resource.
    ISBN: 9781493990122
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  • 44
    Keywords: Pharmaceutical technology ; Bioinformatics ; Pharmaceutical Sciences/Technology ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Miniaturized Checkerboard Assays to Measure Antibiotic Interactions -- High-Throughput Screening for Drug Combinations -- Post-Processing of Large Bioactivity Data -- How to Develop a Drug Target Ontology: KNowledge Acquisition and Representation Methodology (KNARM) -- A Guide to Dictionary-Based Text Mining -- Leveraging Big Data to Transform Drug Discovery -- How to Prepare a Compound Collection Prior to Virtual Screening -- Building a Quantitative Structure-Property Relationship (QSPR) Model -- Isomeric and Conformational Analysis of Small Drug and Drug-Like Molecules by Ion Mobility Mass Spectrometry (IM-MS) -- A Computational Platform and Guide for Acceleration of Novel Medicines and Personalized Medicine -- Omics Data Integration and Analysis for Systems Pharmacology -- Bioinformatics Based Tools and Software in Clinical Research: A New Emerging Area -- Text Mining for Drug Discovery -- Big Data Cohort Extraction for Personalized Statin Treatment and Machine Learning -- Drug Signature Detection Based on L1000 Genomic and Proteomic Big Data -- Drug Effect Prediction by Integrating L1000 Genomic and Proteomic Big Data -- A Bayesian Network Approach to Disease Subtype Discovery
    Abstract: This third edition volume expands on the previous editions with new topics that cover drug discovery through translational bioinformatics, informatics, clinical research informatics, as well as clinical informatics. The chapters discuss new methods to study target identification, genome analysis, cheminformatics, protein analysis, and text mining. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, software workflows, reagents and on-line resources, together with step-by-step, readily reproducible laboratory and computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Bioinformatics and Drug Discovery, Third Edition is a valuable resource for anyone interested in drug design, including academicians (biologists, informaticists and data scientists, chemists, and biochemists), clinicians, and pharmaceutical scientists
    Pages: XI, 324 p. 63 illus., 54 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493990894
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  • 45
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 46
    Keywords: Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp. -- Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance -- Antimicrobial Susceptibility Testing Methods for Acinetobacter spp -- Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii -- Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper Supplemented Liquid Media and on Copper-Containing Surfaces -- A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy -- Transformation of Acinetobacter baumannii: Electroporation -- Methods for Natural Transformation in Acinetobacter baumannii -- Vesicle Mediated Gene Transfer in A. baumannii -- Targeted Gene Replacement in Acinetobacter baumannii -- RecET-Mediated Recombineering in Acinetobacter baumannii -- Methods for Tn-seq Analysis in Acinetobacter baumannii -- Tn7-Based Single Copy Insertion Vectors for Acinetobacter baumannii -- Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii -- A Simple Static Biofilm Assay for Acinetobacter baumannii -- BioFluxTM 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth -- In Vitro Motility Assays for Acinetobacter Species -- Desiccation Tolerance Assays for Acinetobacter baumannii -- Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure -- PBP Isolation and DD-Carboxypeptidase Assay -- Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii -- Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii -- Methods for Detecting N-acyl-homoserine Lactone Production in Acinetobacter baumannii -- Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii -- Skin and Soft Tissue Models for Acinetobacter baumannii Infection -- Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection -- Computational Prediction of sRNA in Acinetobacter baumannii -- Global Metabolic Analyses of Acinetobacter baumannii -- Reverse Vaccinology Approach to Potential Vaccine Candidates against Acinetobacter baumannii
    Abstract: This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study
    Pages: XIV, 338 p. 87 illus., 53 illus. in color. : online resource.
    ISBN: 9781493991181
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  • 47
    Keywords: Neurosciences ; Artificial intelligence ; Social sciences / Data processing ; Neurosciences ; Artificial intelligence ; Computer Appl. in Social and Behavioral Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- Philosophy.-Blended cognition The robotic challenge -- Manipulative Abduction, External Semiotic Anchors, and Blended Cognition -- Practical Reasoning in the Deliberations of an Intelligent Autonomous Agent -- Blended Automation: The Language-Game of Psychoanalytic Automatism and Cybernetic Automata.-Engineering -- Can our Robots rely on an Emotionally Charged Vision-for- Action? An Embodied Model for Neurorobotics -- Modeling psycho-emotional states via neurosimulation of monoamine neuro-Transmitters -- Towards Conversation Envisioning for Cognitive Robots -- Psychology -- The blending of human and autonomous-machine cognition -- Heuristics of Numerical Choice in Economic Contexts -- Anthropology -- Towards autonomous artificial agents? Proposal for a naturalistic activity-based model of (artificial) life -- Logics -- Toward a Logic of Everyday Reasoning -- Index
    Abstract: This edited volume is about how unprejudiced approaches to real human cognition can improve the design of AI. It covers many aspects of human cognition and across 12 chapters the reader can explore multiple approaches about the complexities of human cognitive skills and reasoning, always guided by experts from different but complimentary academic fields. A central concept is explained: blended cognition, the natural skill of human beings for combining constantly different heuristics during their several task-solving activities. Something that was sometimes observed like a problem as “bad reasoning”, is now the central key for the understanding of the richness, adaptability and creativity of human cognition. The topic of this book connects in a significant way with the disciplines of psychology, neurology, anthropology, philosophy, logics, engineering, logics, and AI. In a nutshell: understanding better humans for designing better machines. Any person with interests on natural and artificial reasoning should read this book as a primary source of inspiration and a way to achieve a critical thinking on these topics
    Pages: X, 304 p. 54 illus., 24 illus. in color. : online resource.
    ISBN: 9783030031046
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  • 48
    Keywords: Human Genetics ; Bioinformatics ; Human Genetics ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Chapter1. Strategies for converting RNA to amplifiable cDNA for single-cell RNA sequencing methods -- Chapter2. Integrated Fluidic Circuits for single cell omics and multi-omics applications -- Chapter3. Single-cell DNA-Seq and RNA-Seq in cancer using the C1 system -- Chapter4. Nx1-Seq (Well based Single-Cell Analysis System) -- Chapter5. Quantitation of mRNA transcripts and proteins using the BD Rhapsody™ Single-Cell Analysis System -- Chapter6. An Informative Approach to Single-Cell Sequencing Analysis -- Chapter7. Bionano Genome Mapping: High-Throughput, Ultra-Long Molecule Genome Analysis System for Precision Genome Assembly and Haploid-Resolved Structural Variation Discovery -- Chapter8. Informatics for PacBio long reads -- Chapter10. On-site MinION sequencing
    Abstract: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians
    Pages: VI, 150 p. 56 illus., 51 illus. in color. : online resource.
    ISBN: 9789811360374
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  • 49
    Keywords: Oncology ; Neurosciences ; Toxicology ; Psychiatry ; Cancer Research ; Neurosciences ; Pharmacology/Toxicology ; Psychiatry ; Springer eBooks
    Description / Table of Contents: 1. DUSP3/VHR: A Druggable Dual Phosphatase for Human Diseases -- 2. Oncotic Cell Death in Stroke -- 3. Magnesium Extravaganza: A Critical Compendium of Current Research into Cellular Mg2+ Transporters Other than TRPM6/7 -- 4. Curcumin in Advancing Treatment for Gynecological Cancers with Developed Drug- and Radiotherapy-Associated Resistance -- 5. Correction to Curcumin in Advancing Treatment for Gynecological Cancers with Developed Drug- and Radiotherapy-Associated Resistance
    Abstract: Lucas Falcão Monteiro, Pault Yeison Minaya Ferruzo, Lilian Cristina Russo, Jessica Oliveira Farias, and Fábio Luís Forti: DUSP3/VHR: A Druggable Dual Phosphatase for Human Diseases Kep Yong Loh, Ziting Wang, and Ping Liao: Oncotic Cell Death in Stroke Martin Kolisek, Gerhard Sponder, Ivana Pilchova, Michal Cibulka, Zuzana Tatarkova, Tanja Werner, and Peter Racay: Magnesium Extravaganza: A Critical Compendium of Current Research into Cellular Mg2+ Transporters Other than TRPM6/7 Amir Abbas Momtazi-Borojeni, Jafar Mosafer, Banafsheh Nikfar, Mahnaz Ekhlasi-Hundrieser, Shahla Chaichian, AbolfazlMehdizadehkashi,and Atefeh Vaezi: Curcumin in advancing treatment for gynecological cancers with developed drug- and radiotherapy-associated resistance Amir Abbas Momtazi-Borojeni, Jafar Mosafer, Banafsheh Nikfar, Mahnaz Ekhlasi-Hundrieser, Shahla Chaichian, AbolfazlMehdizadehkashi,and Atefeh Vaezi: Correction to: Curcumin in advancing treatment for gynecological cancers with developed drug- and radiotherapy-associated resistance
    Pages: V, 131 p. 14 illus., 13 illus. in color. : online resource.
    ISBN: 9783030140274
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    Keywords: Pneumology ; Human Physiology ; Neurobiology ; Pneumology/Respiratory System ; Human Physiology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- Methodological Implications and Repeatability of Nasal Nitric Oxide: Relevance for Challenge Studies -- Airway Obstruction in Sleep Apnea Patients -- Effects of Long-Term Exposure to Ambient Air Pollution in Childhood-Adolescence on Lung Function in Adulthood -- Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease -- MicroRNA-9 and Cell Proliferation in Lipopolysaccharide and Dexamethasone-Treated Naïve and Desialylated A549 Cells Grown in Cigarette Smoke Conditioned Medium -- COPD Course and Comorbidities: Are There Gender Differences?- Post-Traumatic Stress Disorder: A Review of Therapeutic Role of Meditation Interventions -- Rehabilitation of Neuromotor Disabilities in Aquatic Microgravity Environment -- Lifestyle Intervention Program for Amelioration of Dysmetabolism in Overweight Women -- Immunization Coverage in Children with Inflammatory Bowel Disease -- Index
    Abstract: This book tackles the role of interrelated conditions in the manifestation and course of pulmonary ailments. The articles run the gamut from basic to clinical science on such topics as airway allergy challenges, pulmonary dysfunction due to exposure to environmental pollution, molecular and functional aspects of smoking in lung carcinogenesis and in obstruction-related respiratory disorders, and others. Attention is directed to the developments in rehabilitative and lifestyle interventions in chronic disabilities, particularly involving neuromotor and stress-related conditions which, with advancing age, may lead to the deterioration of ventilation, with hypoxic sequelae. Enhanced research and awareness concerning the lung health drive better treatments and quality of life. Clinical insights presented in the book underscore a major role played by the science in transmuting biomedical knowledge into a better patient management. The volume is addressed to clinicians, researchers, physiotherapists, and other healthcare professionals engaged in effective patient care and therapy
    Pages: VIII, 90 p. : online resource.
    ISBN: 9783030040253
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