Biochemistry and Biotechnology
Wiley InterScience Backfile Collection 1832-2000
Process Engineering, Biotechnology, Nutrition Technology
The chemolithotroph, Hydrogenomonas eutropha, was tested for its ability to utilize a variety of single nitrogen sources during growth in an atmosphere of H2—O2—CO2 The present data show that H. eutropha can utilize the nitrogen from many, but not all, amino acids, several sulfur-containing amino acids, glucosamine, and two aliphatic amides. The nitrogen concentration that supported maximum growth for NH4Cl, L-glutamate, L-glutamine, urea, and glycine was in the 0.010-0.019M range. H. eutropha failed to remove the nitrogen from primary and secondary amines, eycloleucine, tert-DL-leucine, DL-p-fluorophenylalanine, DL-5-methyltryptophan, creatine, and creatine. This microorganism was able to partially degrade at least six substituted indoles and/or tryptophan catabolites and six substituted imidazoles and/or histidine catabolites. All of a series of 17 dipeptides were able to serve as a nitrogen source for growth in the absence of NH4Cl. Extracts of H. eutropha were able to catalyze the hydrolysis of 16 α-dipeptides, 2 tripetides, a tetrapeptide, a polypeptide, a β-aspartyl peptide, 2 γ-glutamyl peptides, a N-acetyl amino acid, and 4 amino acid amides. These results emphasize the effectiveness of H. eutropha in utilizing a wide diversity of organic nitrogenous compounds containing amino and amide groups, heterocyclic rings, and peptide bonds.
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