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  • 1
    Keywords: Medicine ; Vaccines ; Drug Resistance ; Parasitology ; Pharmaceutical technology ; Infectious Diseases ; Microbiology ; Biomedicine ; Drug Resistance ; Infectious Diseases ; Pharmaceutical Sciences/Technology ; Vaccine ; Parasitology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Prologue -- Introduction -- Part I Determinant features in Leishmaniasis -- Molecular evolution and phylogeny of Leishmania -- The role of reservoirs: canine Leishmaniasis -- Epidemiology of Leishmaniasis in the time of drug resistance -- The role of the immune system in resistance to infection -- Part II Challenges in the diagnosis, treatment and control of Leishmaniasis in the times of drug resistance -- Co-infection with HIV -- Visceral Leishmaniasis -- Tegumentary Leishmaniasis -- Antimony accounted sequel post Kala-Azar dermal Leishmaniasis -- Part III Molecular featrues of drug resistant Leishmania -- Genetic Expression and drug resistance, the role of proteomics -- The role of AMC transporters in drug-resistant Leishmania -- Functional analysis of Leishmania membrane (non AMC) transporters involved in drug resistance -- Part IV Pharmacology and chemotherapy of Leishmaniasis -- Classical vs. novel treatment regimens -- Bioinformatics in Leishmania drug design -- Leishmanis mitochondria, a druggable organelle -- P-glycoprotein-like transporters in Leishmania: a search for reversal agents -- Part V Strategies to circumvent drug resistance in Leishmania -- The relevance of susceptibility tests, breakpoints and markers -- The concept of fitness in Leishmania
    Abstract: Drug resistance is a fundamental factor in treatment failure in diseases like leishmaniasis, although additional factors also play a role in this phenomenon. This volume is the second edition of a well-received book that provides a comprehensive update on the pathology of the disease, as well as on the concept of parasitic drug resistance, its molecular basis, consequences and possible treatments. A valuable resource for scientists both from academia and industry involved in biomedical research and drug design, the book provides the knowledge needed to understand leishmaniasis and to contibute to combatting this disease worldwide
    Pages: XVII, 376 p. 24 illus., 14 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9783319741864
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  • 2
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Natural History of Aromatic L-Amino Acid Decarboxylase Deficiency in Taiwan -- Nitisinone-Induced Keratopathy in Alkaptonuria: A Challenging Diagnosis Despite Clinical Suspicion -- ALG13-CDG with Infantile Spasms in a Male Patient Due to a De Novo ALG13 Gene Mutation -- Liver Failure as the Presentation of Ornithine Transcarbamylase Deficiency in a 13-Month-Old Female -- The Use of d2 and Benton Tests for Assessment of Attention Deficits and Visual Memory in Teenagers with Phenylketonuria -- Asymptomatic Corneal Keratopathy Secondary to Hypertyrosinaemia Following Low Dose Nitisinone and a Literature Review of Tyrosine Keratopathy in Alkaptonuria -- Hyperphenylalaninaemias in Estonia: Genotype–Phenotype Correlation and Comparative Overview of the Patient Cohort Before and After Nation-Wide Neonatal Screening -- Clinical, Biochemical, and Molecular Features in 37 Saudi Patients with Very Long Chain Acyl CoA Dehydrogenase Deficiency -- Novel Missense LCAT Gene Mutation Associated with an Atypical Phenotype of Familial LCAT Deficiency in Two Portuguese Brothers -- Mitochondrial 3-Hydroxy-3-Methylglutaryl-CoA Synthase Deficiency: Unique Presenting Laboratory Values and a Review of Biochemical and Clinical Features -- Extended Experience of Lower Dose Sapropterin in Irish Adults with Mild Phenylketonuria -- Fumarase Deficiency: A Safe and Potentially Disease Modifying Effect of High Fat/Low Carbohydrate Diet -- Early Diagnosed and Treated Glutaric Acidemia Type 1 Female Presenting with Subependymal Nodules in Adulthood -- Mitochondrial Trifunctional Protein Deficiency: Severe Cardiomyopathy and Cardiac Transplantation -- Three Cases of Hereditary Tyrosinaemia Type 1: Neuropsychiatric Outcomes and Brain Imaging Following Treatment with NTBC
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 103 p. 20 illus., 13 illus. in color. : online resource.
    ISBN: 9783662578803
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  • 3
    Keywords: Medicine ; Biomedical Engineering ; Orthopedics ; Biomaterials ; Biomedicine ; Biomedical Engineering/Biotechnology ; Biomaterials ; Orthopedics ; Biomedical Engineering ; Springer eBooks
    Abstract: This book covers the latest progress in the biology and manufacturing of orthopedic biomaterials, as well as key industry perspectives. Topics covered include the development of biomaterial-based medical products for orthopedic applications, anti-infection technologies for orthopedic implants, additive manufacturing of orthopedic implants, and more. This is an ideal book for graduate students, researchers and professionals working with orthopedic biomaterials and tissue engineering. This book also: Provides an industry perspective on technologies to prevent orthopedic implant related infection Thoroughly covers how to modulate innate inflammatory reactions in the application of orthopedic biomaterials Details the state-of-the-art research on 3D printed porous bone constructs
    Pages: VIII, 496 p. 174 illus., 126 illus. in color. : online resource.
    ISBN: 9783319895420
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  • 4
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Tertiary Lymphoid Structures among the World of Non-Canonical Ectopic Lymphoid Organizations -- Cellular and Vascular Components of Tertiary Lymphoid Structures -- Meningeal Immunity, Drainage, and Tertiary Lymphoid Structure Formation -- Development of Tools for the Selective Visualization and Quantification of TLS-Immune Cells on Tissue Sections -- A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures -- Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer -- Defining High Endothelial Venules and Tertiary Lymphoid Structures in Cancer -- Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure using Laser Capture Microdissection -- Quantifying Tertiary Lymphoid Structure-Associated Genes in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues -- Generation of Recombinant Monoclonal Antibodies from Single B Cells Isolated from Synovial Tissue of Rheumatoid Arthritis Patients -- Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations -- Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues -- Exploring the Role of Tertiary Lymphoid Structures using a Mouse Model of Bacteria-Infected Lungs -- Identification and Characterization of Tertiary Lymphoid Structures in Murine Melanoma -- Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma -- Targeting Tertiary Lymphoid structures for Tumor Immunotherapy
    Abstract: This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications
    Pages: XII, 289 p. 38 illus., 34 illus. in color. : online resource.
    ISBN: 9781493987092
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  • 5
    Keywords: Medicine ; Biomedical Engineering ; Microbiology ; Mycology ; Sustainable development ; Biomedicine ; Biomedical Engineering/Biotechnology ; Mycology ; Sustainable development ; Applied Microbiology ; Eukaryotic Microbiology ; Springer eBooks
    Description / Table of Contents: Part 1. Sustainable Cultivation and Conservation Strategies of Fungi -- Chapter 1. Cultivation, conservation and medicinal significance of macro-fungi -- Chapter 2. Fungi as biocontrol agent: an alternate to chemicals -- Chapter 3. Incredible Role of Fungi in various Fields for sustainable development -- Chapter 4. The biological promises of endophytic Muscodor species -- Chapter 5. Slime Moulds: The Tiny Charmers -- Chapter 6. Fungal Biotechnology: Role and Aspects -- Part 2. Sustainable Aspects of Fungi in Agriculture -- Chapter 7. Fungal Endophytes: Role in Sustainable Agriculture -- Chapter 8. Fungal endophytes and their secondary metabolites - Role in sustainable agriculture -- Chapter 9. Agricultural important microorganism- Sustainability from rhizosphere to bioformulation as weapon for biological control -- Chapter 10. Recruit the Plant Pathogen for Weed Management: Bioherbicide - A Sustainable Strategy -- Chapter 11. Mycopesticides: Fungal based pesticides for sustainable agriculture -- Chapter 12. Multifactorial role of Arbuscular Mycorrhizae in Agroecosystem -- Chapter 13. Strategic approaches for applications of entomopathogenic fungi to counter insecticide resistance in agriculturally important insect pests -- Chapter 14. Arbuscular Mycorrhizal Fungi as phosphate fertilizer for crop plants and their role in bioremediation of heavy metals -- Chapter 15. Fungal community in mitigating impacts of drought in plants -- Chapter 16. Role of Fungi as Biocontrol Agents for the Control of Plant Diseases in Sustainable Agriculture -- Chapter 17. Fungal disease management in chickpea: current status and future prospects -- Chapter 18. Recent approaches for late blight disease management of potato caused by Phytophthora infestans -- Part 3. Fungi for sustainable Industrial and Environmental Aspects -- Chapter 19. Recent advances and industrial applications of microbial xylanases: A review -- Chapter 20. Bio-Valorization of Dairy Whey for Bio-Ethanol by Stress Tolerance Yeast -- Chapter 21. Nutritional quality attributes of edible gasteroid wild mushroom Astraeus hygrometricus -- Chapter 22. Biotechnology of Fungal Lipases -- Chapter 23. Biopigments: Fungal Pigments -- Chapter 24. Bioethanol Production from Renewable Biomass by Yeast -- Chapter 25. Fungi Inhabiting in Hypersaline Conditions - An Insight -- Chapter 26. Carbon sequestration and the significance of soil fungi in the process -- Chapter 27. Fungal infections and Intervention in Diabetic complication -- Part 4. Bioconversion Technologies of Fungi -- Chapter 28. Arbuscular Mycorrhizal Fungi: Effects on secondary metabolite production in medicinal plants -- Chapter 29. Endophytic fungi: Carrier of potential antioxidants -- Chapter 30. Current perspectives of Endophytic fungi in Sustainable Development -- Chapter 31. Marine Fungi for Sustainable Development -- Chapter 32. Fungi-mediated biodeterioration of household materials, libraries, cultural heritage and its sustainable conservation -- Chapter 33. Co-cultivation strategies to induce de-novo synthesis of novel chemical scaffolds from cryptic secondary metabolite gene clusters -- Part 5. Modern Biotechnological Interventions on Fungi -- Chapter 34. Computational approaches to understand the genome and protein sequences of fungi -- Chapter 35. Bioprospecting of endophytic fungi for bioactive compounds -- Chapter 36. Bioactive Compounds from Endophytic Fungi -- Chapter 37. Bioactive potential of non-conventional edible wild mushroom Amanita -- Chapter 38. Fungus Monascus -fermented red yeast rice (RYR): Natural therapeutic statin source or mycotoxin? -- Chapter 39. Nanoparticles from Fungi (Myconanoparticles)
    Abstract: This book illustrates the multiple roles of fungi in everyday life. Fungi are the large group of organisms with tremendous diversity and economic importance. Their ability to produce commercially efficient useful products makes them the vulnerable sustainable tool for the future generation. This book describes a systems approach and provides a means to share the latest developments and advances about the benefits of fungi including their wide application, traditional uses, modern practices, along with designing of strategies to harness their potential. The chapters are organized with data, providing information related to different sustainable aspects of fungi in agriculture, its cultivation and conservation strategies, industrial and environmental utilization, advanced bioconversion technologies and modern biotechnological interventions. Updated information and current opinion related to its application for sustainable agriculture, environment, and industries as futuristic tools have been presented and discussed in different chapters. The book also elucidates a comprehensive yet a representative description of the challenges associated with the sustained application of fungi to achieve the goals of sustainability
    Pages: X, 779 p. 141 illus., 41 illus. in color. : online resource.
    ISBN: 9789811303937
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  • 6
    Keywords: Medicine ; Pharmaceutical technology ; Ophthalmology ; Biomedicine ; Pharmaceutical Sciences/Technology ; Ophthalmology ; Springer eBooks
    Description / Table of Contents: Part I- Introduction and basic concept of drug delivery for retina and posterior segment disease -- Anatomy and physiology of retina and posterior segment of the eye -- Pharmacotherapy of diabetic macular edema and retinopathy -- Drug delivery to posterior segment of eye: Conventional delivery strategies, their barriers and restrictions -- Penetration routes to retina and posterior segment -- Diabetic Retinopathy: Pathogenesis, Treatment, and Complications -- PART II-Nanotechnology based formulations for retina and posterior segment disease -- Liposomes for retina and posterior segment disease -- Nano/microparticles for retina and posterior segment disease -- Non-viral delivery systems for gene therapy for retina and posterior segment disease -- Oxidative stress in ocular disorders: Exploring the link to pesticide exposure and potential for using nanotechnology for antioxidant delivery -- Advances in the microbial infection in cornea and role of nanotechnology to cure ocular surface -- Nanomedicine based delivery to the posterior segment of eye: Brighter tomorrow -- Part III-Transscleral iontophoresis for retina and posterior segment disease -- Transscleral drug delivery to retina and posterior segment disease -- Colloidal carrier systems transscleral drug delivery -- Transscleral Iontophoretic Drug Delivery for Treating Retinal Diseases -- Part IV-Implant formulation for posterior eye segment -- Biodegradable polymeric implants for retina and posterior segment disease -- Nanomedicine based gene delivery for the retina and posterior segment diseases -- Part V-Prodrug strategies for retina and posterior segment disease -- Transporter targeted Pro-drug approach for retina and posterior segment disease -- Lipid Prodrug based delivery for retina and posterior segment disease -- Injectable Pro-drugs approach for retina and posterior segment disease -- Stereoisomeric dipeptide Pro-drug approach for retina and posterior segment disease -- Part VI-Other advances for retina and posterior segment diseases -- Receptor targeted Pro-drug approach for retina and posterior segment disease -- Intravitreal injection drug delivery for retina and posterior segment disease: Challenges and Future ahead -- Thermoresponsive gels drug delivery for retina and posterior segment disease -- Peptide synthesis and delivery for retina and posterior segment disease -- Corneal haze, refractive surgery and its implications on choroidal neovascularisation
    Abstract: This book addresses the issues relating to a wide variety of ocular diseases from which millions of people suffer. Long-term challenges include visual impairment and ocular blindness. Certain ocular diseases are quite rare, whereas others, such as cataracts, age-related macular degeneration (AMD), and glaucoma, are very common, especially in the aging population. A rapid expansion of new technologies in ocular drug delivery and new drug candidates, including biologics, to treat these challenging diseases in the retina and posterior segments of the eye have recently emerged. These approaches are necessary because the eye has many unique barriers to drug delivery. Thus, this timely reference Drug Delivery for the Retina and Posterior Segment Disease compiles and analyzes recent advances in the research and development of drug delivery systems for retina and posterior segment diseases of the eye, with an emphasis on the use of implantable devices, iontophoresis as well as micro- and nanoparticles
    Pages: XIX, 494 p. 68 illus., 47 illus. in color. : online resource.
    ISBN: 9783319958071
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  • 7
    Keywords: Medicine ; Medical Microbiology ; Infectious Diseases ; Environmental Health ; Biomedicine ; Medical Microbiology ; Infectious Diseases ; Environmental Health ; Springer eBooks
    Description / Table of Contents: 1. Chemical characterization -- 2. Types of application -- 2a. Approval by ECHA -- 2b. Approval by EPA -- 2c. Amounts per type of application (overall environmental impact) -- 3. Spectrum of antimicrobial activity -- 3a. Bactericidal activity -- 3b. Fungicidal activity -- 3c. Mycobactericidal activity -- 3d. Virucidal activity -- 3e. Sporicidal activity -- 4. Natural resistances -- 5. Adaptation after exposure -- 5a. Per type of typical application -- 6. 7. Cross-resistance to other biocidal agents -- 8. Cross-resistances to antibiotics -- 9. Proposal for responsible use (antiseptic stewardship)
    Abstract: VVarious antiseptic agents, such as chlorhexidine, are used for different applications, e.g. in healthcare, veterinary medicine, animal production and household products, including cosmetics. However, not all antiseptic agents provide significant health benefits, especially in some products used in human medicine (alcohol-based hand rubs, antimicrobial soaps). While some products (antimicrobial soaps, surface disinfectants, instrument disinfectants, wound antiseptics) may contain one or more biocidal agents with a comparable antimicrobial efficacy but large differences in their potential for microbial adaptation and tolerance. An increased bacterial resistance has been described for various antimicrobial agents, sometimes including a cross-resistance to antibiotics. The book is the first comprehensive reference resource on antiseptic agents, including their efficacy, natural and acquired resistance, adaptation, and cross-resistance. It also discusses their and appropriate use in terms of a balance between their efficacy and the risk of acquired bacterial resistance / tolerance. Focusing on human and veterinary medicine and household products, it helps readers make informed decisions concerning against antiseptic products based on their composition. The book contributes to reduce any unnecessary selection pressure towards emerging pathogens and to keep the powerful antiseptic agents for all those applications that have a clear benefit (e.g. reduction of healthcare-associated infection)
    Pages: XLI, 694 p. 20 illus., 17 illus. in color. : online resource.
    ISBN: 9783319987859
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  • 8
    Keywords: Medicine ; Neurosciences ; Psychiatry ; Neurobiology ; Biomedicine ; Neurosciences ; Psychiatry ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Developmental Contributors to Trauma Response: The Importance of Sensitive Periods, Early Environment, and Sex Differences -- MicroRNAs in Post-traumatic Stress Disorder -- Animal Models of PTSD: A Critical Review -- Integrating NIMH Research Domain Criteria (RDoC) into PTSD Research -- Neurocognition in PTSD: Treatment Insights and Implications -- Neurobiological Programming of Early Life 1 Stress: Functional Development 2 of Amygdala-Prefrontal Circuitry 3 and Vulnerability for Stress-Related 4 Psychopathology -- Subanesthetic dose Ketamine in Posttraumatic Stress Disorder: A Role for Reconsolidation during Trauma-focused Psychotherapy? -- Emerging approaches to neurocircuits in PTSD and TBI: Imaging the interplay of neural and emotional trauma -- PTSD and sleep: focus on fear learning processes -- The future of contextual fear learning for PTSD research: A methodological review of neuroimaging studies -- Dissociative subtype PTSD; research update on clinical and neurobiological studies -- Does Anhedonia Presage Increased Risk of Posttraumatic Stress Disorder?
    Abstract: This volume focuses on the behavioral neuroscience that supports our understanding of the neurobiology of trauma risk and response. The collection of articles focuses on both preclinical and clinical reviews of (1) state-of-the-art knowledge of mechanisms of posttraumatic stress disorder (PTSD) and co-occurring disorders, (2) the biological and psychological constructs that support risk and resiliency for trauma disorders, and (3), novel treatment strategies and therapeutics on the horizon
    Pages: X, 265 p. 18 illus., 6 illus. in color. : online resource.
    ISBN: 9783319948249
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  • 9
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Husbandry, General Care, and Transportation of Xenopus laevis, and Xenopus tropicalis -- Generation and Care of Xenopus laevis and Xenopus tropcalis Embryos -- Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering -- Targeted Genome Engineering in Xenopus using the Transcription activator-Like Effector Nuclease (TALEN) Technology -- Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis -- BATCH-GE: Analysis of NGS Data for Genome Editing Assessment -- A Simple Knock-In System for Xenopus via Microhomology-Mediated End Jointing Repair -- How to Generate Non-Mosaic CRSPR/Cas9 Mediated Knock-In and Mutations F0 Xenopus through the Host-Transfer Technique -- Targeted Electroporation in the CNS in Xenopus Embryos -- Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus retina -- Cancer Models in Xenopus tropicalis by CRISPR/Cas 9 Mediated Knockout of Tumor Suppressors -- CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis -- Quantitative Proteomics of Xenopus Embryos I, Sample Preparation -- Quantitative Proteomics for Xenopus Embryos II, Data Analysis -- Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System -- X-FaCT: Xenopus-Fast Clearing Technique -- Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry -- Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis -- Evaluating Blood Cell Populations in Xenopus using Flow Cytometry and Differential Counts by Cytospin -- Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells -- Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis
    Abstract: This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model
    Pages: XI, 302 p. 68 illus., 57 illus. in color. : online resource.
    ISBN: 9781493987849
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  • 10
    Keywords: Medicine ; Neurosciences ; Medicinal Chemistry ; Psychopharmacology ; Biomedicine ; Neurosciences ; Psychopharmacology ; Medicinal Chemistry ; Springer eBooks
    Description / Table of Contents: Preface -- Chemistry and structure-activity relationships of psychedelics -- Hallucinogens and serotonin 5-HT2A receptor-mediated signaling pathways -- Effects of hallucinogens on neuronal activity -- Interactions of Hallucinogens with the Glutamatergic System:℗ Permissive Network Effects Mediated Through Cortical Layer V Pyramidal Neurons -- The effects of hallucinogens on gene expression -- Effect of Hallucinogens on Unconditioned Behavior -- Hallucinogens in Drug Discrimination -- Phenomenology, structure and dynamic of psychedelic states -- Serotonergic hallucinogen-induced visual perceptual alterations -- New world tryptamine hallucinogens and the neuroscience of ayahuasca -- Experimental psychosis research and schizophrenia - similarities and dissimilarities in psychopathology -- A review of Hallucinogen Persisting Perception Disorder (HPPD) and an exploratory study of subjects claiming symptoms of HPPD -- Therapeutic applications of classic hallucinogens -- Classic Hallucinogens and Mystical Experiences: Phenomenology and Neural Correlates
    Abstract: This volume brings together the latest basic and clinical research examining the effects and underlying mechanisms of psychedelic drugs. Examples of drugs within this group include LSD, psilocybin, and mescaline. Despite their structural differences, these compounds produce remarkably similar experiences in humans and share a common mechanism of action.℗ Commonalities among the substances in this family are addressed both at the clinical and phenomenological level and at the basic neurobiological mechanism level. To the extent possible, contributions relate the clinical and preclinical findings to one another across species. The volume addresses both the risks associated with the use of these drugs and the potential medical benefits that might be associated with these and related compounds
    Pages: X, 430 p. 196 illus., 158 illus. in color. : online resource.
    ISBN: 9783662558805
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  • 11
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: First Successful Conception Induced by a Male Cystinosis Patient -- Glutaric Acidemia Type 1: A Case of Infantile Stroke -- Treatment of Depression in Adults with Fabry Disease -- Mutations in GMPPB Presenting with Pseudometabolic Myopathy -- Heterogeneous Phenotypes in Lipid Storage Myopathy Due to ETFDH Gene Mutations -- Successful Management of Pregnancies in Patients with Inherited Disorders of Ketone Body Metabolism -- Improvement of Fabry Disease-Related Gastrointestinal Symptoms in a Significant Proportion of Female Patients Treated with Agalsidase Beta: Data from the Fabry Registry -- Ketone Bodies as a Possible Adjuvant to Ketogenic Diet in PDHc Deficiency but Not in GLUT1 Deficiency -- GM2 Activator Deficiency Caused by a Homozygous Exon 2 Deletion in GM2A -- Effect of Lorenzo’s Oil on Hepatic Gene Expression and the Serum Fatty Acid Level in abcd1-Deficient Mice -- Introduction of a Simple Second Tier Screening Test for C5 Isobars in Dried Blood Spots: Reducing the False Positive Rate for Isovaleric Acidaemia in Expanded Newborn Screening -- Open-Label Single-Sequence Crossover Study Evaluating Pharmacokinetics, Efficacy, and Safety of Once-Daily Dosing of Nitisinone in Patients with Hereditary Tyrosinemia Type 1 -- A Rapid Two-Step Iduronate-2-Sulfatatse Enzymatic Activity Assay for MPSII Pharmacokinetic Assessment -- An Unexplained Congenital Disorder of Glycosylation-II in a Child with Neurohepatic Involvement, Hypercholesterolemia and Hypoceruloplasminemia -- Peripheral Neuropathy, Episodic Rhabdomyolysis, and Hypoparathyroidism in a Patient with Mitochondrial Trifunctional Protein Deficiency
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder. The chapter 'Open-Label Single-Sequence Crossover Study Evaluating Pharmacokinetics, Efficacy, and Safety of Once-Daily Dosing of Nitisinone in Patients with Hereditary Tyrosinemia Type 1 (HT-1)' is open access under a CC BY 4.0 license via link.springer.com
    Pages: VI, 105 p. 20 illus., 7 illus. in color. : online resource.
    ISBN: 9783662566107
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  • 12
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Abstract: This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.℗ Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes
    Pages: XII, 234 p. 53 illus., 42 illus. in color. : online resource.
    ISBN: 9781493977246
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  • 13
    Keywords: Medicine ; Cancer Research ; Endocrinology ; Oncology ; Biomedicine ; Cancer Research ; Oncology ; Endocrinology ; Springer eBooks
    Description / Table of Contents: Section: The Nature of Pancreatic Cancer -- Cell Cycle Machinery and Its Alterations in Pancreatic Cancer -- Animal modelling of Pancreatitis-to-Cancer Progression -- Stromal Inflammation in Pancreatic Cancer: Mechanisms and Translational Applications -- Familial Pancreatic Cancer -- Section: Clinical Management of Pancreatic Cancer -- Therapeutic Endoscopy in the Management of Pancreatic Cancer -- Controversies in Pathology Reporting and Staging -- Staging and Postoperative Outcomes Using the International Study Group of Pancreatic Surgery (ISGPS) Classifications -- Borderline Respectable Pancreatic Cancer -- New Japanese Classification of Pancreatic Cancer -- Arterial Resection in Pancreatic Cancer -- Treatment of Recurrent Pancreatic Cancer After Surgery -- Neoadjuvant Chemotherapy in Pancreatic Cancer -- Differential Therapy Based on Tumor Heterogeneity in Pancreatic Cancer -- Neoadjuvant Chemoradiation for Operable Pancreatic Cancer: the Importance of Local Disease Control -- Section: New Directions -- Development of Novel Therapeutic Response Biomarkers -- Clinical Applications of Genomics and Proteomics in Pancreatic Cancer -- Approaching Pancreatic Cancer Phenotypes via Metabolomics -- Circulating Tumour Cells -- Cancer Exosomes for Early Pancreatic Cancer Diagnosis and Role Metastasis -- Metabolism in Pancreatic Cancer -- Secondary Screening for Inherited Pancreatic Ductal Adenocarcinoma -- Role of Radiotherapy in Locally Advanced Pancreatic Cancer -- Evolution of Pancreatic Cancer Surgery -- Multiparameter Modalities for the Study of Patients in the Setting of Individualized Medicine -- Epigenetic Pharmacology -- Precision Medicine Based on Next Generation Sequencing and Master Controllers
    Abstract: In organizing the second edition of this renowned Handbook, Dr. Neoptolemos and his co-editors have produced and updated a revised edition to the distinguished Major Reference Work devoted to pancreatic cancer. Like its preceding edition, the second edition continues to have a widespread appeal among clinicians, pathologists and basic scientists, who are now struggling to understand this complex and rapidly expanding field. Because of the recent and vast growth in both the clinical and scientific research being done in pancreatic cancer, (there is currently an unprecedented investment by academia and industry in this field), each research’s knowledge of other specialty areas outside his or her own is often quite limited. The aim of the new edition is to place the tangible advances, including new developments in surgical approaches with regards to resection techniques, the state of laparoscopic approaches, the growing impact of surgical approaches in the management of recurrent pancreatic cancer, controversies in the management of IMPN as the precursor lesion for PDAC and others – readily at hand. The second edition focuses on advances that will not become dated, and the editors have chosen authors, who are the very best in each area
    Pages: 244 illus., 177 illus. in color. eReference. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493971930
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  • 14
    Keywords: Medicine ; Laboratory Medicine ; Anesthesiology ; Emergency Medicine ; General practice (Medicine) ; Critical care medicine ; Internal Medicine ; Biomedicine ; Laboratory Medicine ; Anesthesiology ; Emergency Medicine ; Intensive / Critical Care Medicine ; General Practice / Family Medicine ; Internal Medicine ; Springer eBooks
    Description / Table of Contents: I. Introduction -- II. Methodology and analytical techniques -- III. Clinical applications -- IV. Legal and organizational framework -- V. Areas of application.-VI. Quality assurance -- VII. Development trends
    Abstract: The underlying technology and the range of test parameters available are evolving rapidly. The primary advantage of POCT is the convenience of performing the test close to the patient and the speed at which test results can be obtained, compared to sending a sample to a laboratory and waiting for results to be returned. Thus, a series of clinical applications are possible that can shorten the time for clinical decision-making about additional testing or therapy, as delays are no longer caused by preparation of clinical samples, transport, and central laboratory analysis. Tests in a POC format can now be found for many medical disciplines including endocrinology/diabetes, cardiology, nephrology, critical care, fertility, hematology/coagulation, infectious disease and microbiology, and general health screening. Point-of-care testing (POCT) enables health care personnel to perform clinical laboratory testing near the patient. The idea of conventional and POCT laboratory services presiding within a hospital seems contradictory; yet, they are, in fact, complementary: together POCT and central laboratory are important for the optimal functioning of diagnostic processes. They complement each other, provided that a dedicated POCT coordination integrates the quality assurance of POCT into the overall quality management system of the central laboratory. The motivation of the third edition of the POCT book from Luppa/Junker, which is now also available in English, is to explore and describe clinically relevant analytical techniques, organizational concepts for application and future perspectives of POCT. From descriptions of the opportunities that POCT can provide to the limitations that clinician’s must be cautioned about, this book provides an overview of the many aspects that challenge those who choose to implement POCT. Technologies, clinical applications, networking issues and quality regulations are describ ed as well as a survey of future technologies that are on the future horizon. The editors have spent considerable efforts to update the book in general and to highlight the latest developments, e.g., novel POCT applications of nucleic acid testing for the rapid identification of infectious agents. Of particular note is also that a cross-country comparison of POCT quality rules is being described by a team of international experts in this field
    Pages: XXX, 445 p. 100 illus. in color. : online resource.
    ISBN: 9783662544976
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  • 15
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: EM Tomography of LINC Complexes -- Recombinant Purification of the Periplasmic Portion of the LINC Complex -- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis -- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin -- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID -- Using Nesprin Tension Sensors to Measure Force on the LINC Complex -- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei -- Direct Force Probe for Nuclear Mechanics -- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning -- Assembly and use of a Microfluidic Device to Study Cell Migration in Confined Environments -- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy -- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-resolution Imaging -- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes during Development of Caenorhabditis elegans -- High-resolution Imaging Methods to Analyze LINC Complex Function during Drosophila Muscle Development -- Computational Methods for Studying the Plant Nucleus -- Investigation of Nuclear Periphery Protein Interactions in Plants using the Membrane Yeast Two-Hybrid (MbY2H) System -- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components -- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice -- An In Vitro System to Measure the Positioning, Stiffness, and Rupture of the Nucleus in Skeletal Muscle -- Functional Analysis of LINC Complexes in Skin -- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer -- Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
    Abstract: This book details the most comprehensive, up-to –date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome
    Pages: XI, 274 p. 42 illus., 34 illus. in color. : online resource.
    ISBN: 9781493986828
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  • 16
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Favourable Outcome in Two Pregnancies in a Patient with 3-Hydroxy-3-Methylglutaryl-CoA Lyase Deficiency -- Lysosomal Acid Lipase Deficiency in 23 Spanish Patients: High Frequency of the Novel c.966+2T〉G Mutation in Wolman Disease -- Guanidinoacetate Methyltransferase Activity in Lymphocytes, for a Fast Diagnosis -- Galactose Epimerase Deficiency: Expanding the Phenotype -- Development and Psychometric Evaluation of the MetabQoL 1.0: A Quality of Life Questionnaire for Paediatric Patients with Intoxication-Type Inborn Errors of Metabolism -- Widening the Heterogeneity of Leigh Syndrome: Clinical, Biochemical, and Neuroradiologic Features in a Patient Harboring a NDUFA10 Mutation -- Normal Neurological Development During Infancy Despite Massive Hyperammonemia in Early Treated NAGS Deficiency -- Dihydropyrimidine Dehydrogenase Deficiency: Metabolic Disease or Biochemical Phenotype? -- Potential Misdiagnosis of Hyperhomocysteinemia due to Cystathionine Beta-Synthase Deficiency During Pregnancy -- Clinical and Molecular Variability in Patients with℗ PHKA2℗ Variants and Liver Phosphorylase b Kinase Deficiency -- Hyperphenylalaninemia Correlated with Global Decrease of Antioxidant Genes Expression in White Blood Cells of Adult Patients with Phenylketonuria -- The Impact of Fabry Disease on Reproductive Fitness -- Neonatal-Onset Hereditary Coproporphyria: A New Variant of Hereditary Coproporphyria -- Treatment Adherence and Psychological Wellbeing in Maternal Carers of Children with Phenylketonuria (PKU) -- Systematic Review and Meta-analysis of Intelligence Quotient in Early-Treated Individuals with Classical Galactosemia
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 123 p. : online resource.
    ISBN: 9783662563595
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  • 17
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    New York, NY : Springer
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Optimization of In Situ Hybridization and Co--Expression Analysis in the Central Nervous System -- In Situ Hybridization Methods for RNA Visualization in C. elegans -- Multicolored Visualization of Transcript Distributions in Drosophila Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos -- Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections -- Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) -- One FISH, dFISH, three FISH:℗ Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians -- Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos -- Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain -- Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon -- High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond -- Analysis of Embryonic Gene Expression Patterns in the Hagfish -- Gene Expression Analysis of Lamprey Embryos -- Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians -- Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization -- Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs -- Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry -- Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections -- Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH -- Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish -- Multiplex Fluorescent RNA In Situ Hybridization via RNAscope -- Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy -- Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue -- Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus -- Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans -- In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues -- Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts -- Characterizing Cellular Identity at One Cell Resolution -- Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues -- ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments
    Abstract: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms,℗ and applications.℗ The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. ℗ Essential and authoritative, In Situ Hybridization Methods℗ provides detailed protocols for newcomers to ISH,℗ and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications
    Pages: XX, 592 p. 109 illus., 94 illus. in color. : online resource.
    ISBN: 9781493923038
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  • 18
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Establishing New Cut-Off Limits for Galactose 1-Phosphate-Uridyltransferase Deficiency for the Dutch Newborn Screening Programme -- Four Years of Diagnostic Challenges with Tetrahydrobiopterin Deficiencies in Iranian Patients -- Endurance Exercise Training in Young Adults with Barth Syndrome: A Pilot Study -- Hydroxysteroid 17-Beta Dehydrogenase Type 10 Disease in Siblings -- Reliable Diagnosis of Carnitine Palmitoyltransferase Type IA Deficiency by Analysis of Plasma Acylcarnitine Profiles -- Relationships Between Childhood Experiences and Adulthood Outcomes in Women with PKU: A Qualitative Analysis -- The Effect of S-Adenosylmethionine on Self-Mutilation in a Patient with Lesch–Nyhan Disease -- Low Protein Formula: Consequences of Quantitative Effects of Pre-analytical Factors on Amino Acid Concentrations in Plasma of Healthy Infants -- A Multiplatform Metabolomics Approach to Characterize Plasma Levels of Phenylalanine and Tyrosine in Phenylketonuria -- Japanese Male Siblings with 2-Methyl-3-Hydroxybutyryl-CoA Dehydrogenase Deficiency (HSD10 Disease) Without Neurological Regression -- Newborn Screening for Vitamin B6 Non-responsive Classical Homocystinuria: Systematical Evaluation of a Two-Tier Strategy -- Management of an LCHADD Patient During Pregnancy and High Intensity Exercise -- Rare Case of Hepatic Gaucheroma in a Child on Enzyme Replacement Therapy -- Newborn Screening Programmes in Europe, Arguments and Efforts Regarding Harmonisation: Focus on Organic Acidurias -- Whole Exome Sequencing Identifies the Genetic Basis of Late-Onset Leigh Syndrome in a Patient with MRI but Little Biochemical Evidence of a Mitochondrial Disorder
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 30 illus., 15 illus. in color. : online resource.
    ISBN: 9783662543856
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  • 19
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Tachistoscopic Viewing and Dichotic Listening -- Split-Brain Human Subjects -- Eye and Ear Preferences -- Olfactory Lateralization -- Hand, Limb and Other Motor Preferences -- Lateralization in Invertebrates -- Unilateral Lesions -- Pharmacological Agents and Electrophysiological Techniques -- Tract Tracing and Histological Techniques -- Brain Mapping Using the Immediate Early Gene Zenk -- Optogenetic Methods to Study Lateralized Synaptic Function -- Transcranial Magnetic Stimulation -- Electroencephalographic Asymmetries in Human Cognition -- Non-Invasive Imaging Technologies in Primates -- Imaging Techniques in Insects -- Genetics of Human Handedness and Laterality -- Genetic and Transgenic Approaches to Study Zebrafish Brain Asymmetry and Lateralized Behavior -- Methods to Study Nervous System Laterality in the Caenorhabditis elegans Model System -- Manipulation of Strength of Cerebral Lateralization via Embryonic Light Stimulation in Birds -- Effects of Steroid Hormones -- Reversals of Bodies, Brains and Behavior
    Abstract: This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Research in this field increases our understanding of various brain functions in humans, other vertebrate species, and invertebrates. The book is divided into five parts: behavioral methods; neurobiological methods; electroencephalographic, imaging, and neuro-stimulation methods; genetic techniques; and development of lateralization. Part I addresses measuring lateralization by scoring behavior induced by inputs to one or the other side of the brain in a range of species. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods. Part III looks at imaging techniques, electroencephalographic techniques, and transcranial stimulation to reveal lateralization. Part IV describes techniques used to study the role of genes in the development and establishment of brain asymmetry in humans and other species. Lastly, Part V refers to methods used in the study of development of lateralization through the manipulation of sensory exposure, hormone levels, and in model systems. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Cutting-edge and thorough, Lateralized Brain Function: Methods in Human and Non-Human Species is a valuable resource for investigating lateralization in a broad range of species and provides excellent advice for both new and veteran researchers
    Pages: XIV, 710 p. 136 illus., 94 illus. in color. : online resource.
    ISBN: 9781493967254
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  • 20
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Library Construction for High-Throughput Mobile Element Identification and Genotyping -- The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements -- Determining Epigenetic Targets: A Beginner’s Guide to Identifying Genome Functionality Through Database Analysis -- Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C -- Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method -- 5-Hydroxymethylcytosine Profiling in Human DNA -- Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach -- Correcting for Sample Heterogeneity in Methylome-Wide Association Studies -- Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations -- Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine -- Analysis of Imprinted Gene Regulation -- Statistical Methods for Methylation Data
    Abstract: This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity
    Pages: XII, 206 p. : online resource.
    Edition: 1st ed. 2017.
    ISBN: 9781493969036
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  • 21
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Cellular Fractionation and Isolation of Chromatin-associated RNA -- Knockdown of Nuclear-located Enhancer RNAs and Long ncRNAs using Locked Nucleic Acid GapmeRs -- Visualization of Enhancer-derived Non-coding RNA -- UV-RNA Immunoprecipitation (UV-RIP) Protocol in Neurons -- Mapping Long non-coding RNA Chromatin Occupancy using Capture Hybridization Analysis of RNA Targets (CHART) -- Detecting long-range Enhancer-promoter Interactions by Quantitative Chromosome Conformation Capture -- Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET) -- Identification of Transcribed Enhancers by Genome-wide Chromatin Immunoprecipitation Sequencing -- Global Run-On sequencing (GRO-seq) -- Computational Approaches for Mining GRO-seq Data to Identify and Characterize Active Enhancers -- Evaluating the Stability of mRNAs and Non-coding RNAs -- A Novel Method to Quantify RNA-protein Interactions in situ using FMTRIP and Proximity Ligation -- In silico Promoter Recognition from deepCAGE data -- Bioinformatics Pipeline for Transcriptome Sequencing Analysis -- CRISPR/Cas-9 Genome Editing in Embryonic Stem Cells -- Targeted Gene Activation Using RNA-Guided Nucleases
    Abstract: This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field
    Pages: XI, 252 p. 51 illus., 43 illus. in color. : online resource.
    ISBN: 9781493940356
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  • 22
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Overview of the Development of the Mouse Mammary Gland; A Comparison of Mammary Gland Development in Mouse and Humans -- Prenatal Mammary Gland Development in the Mouse - Research Models and Techniques for its Study from Past to Present -- Pubertal Mammary Gland Development: Elucidation Of In Vivo Morphogenesis Using Murine Models -- Analysis of Mammary Gland Phenotypes by Transplantation of the Genetically Marked Mammary Epithelium -- Pubertal Ductal Morphogenesis: Isolation and Transcriptome Analysis of the Terminal End Bud -- RNA Profiling of Non-Cultured Fibroblasts Isolated from Pubertal Mouse Mammary Gland Sections -- Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death -- Contractility Assay for Established Myoepithelial Cell Lines -- The use of 3D Culture of Primary Mammary Epithelial Cells to Define Molecular Entities Required for Acinus Formation. Analysing MAP Kinase Phosphatases -- A 3D Fibroblast-Epithelium Co-culture Model for Understanding Micro-Environmental Role in Branching Morphogenesis of the Mammary Gland -- RNA Sequencing Analysis Reveals Expression of a Transient EMT Profile During the Early During Organization of MCF10A Cells in 3D -- A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells -- Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue -- Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate -- Lineage Tracing of Mammary Stem and Progenitor Cells -- Assessment of Novel Proteins by Tissue Microarray Technology -- Patient-Derived Xenografts of Breast Cancer
    Abstract: This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists, and those with an interest in molecular mechanisms underlying breast cancer
    Pages: XI, 358 p. 62 illus., 32 illus. in color. : online resource.
    ISBN: 9781493964758
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  • 23
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: The Immortal Senescence -- Senescence Phenotypes Induced by Ras in Primary Cells -- Cellular Model of p21-Induced Senescence -- Detecting Markers of Therapy-Induced Senescence in Cancer Cells -- Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence -- Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence -- RT-qPCR Detection of Senescence-Associated Circular RNAs -- Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy -- Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis -- Sudan Black B, the Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells -- Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts -- Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence -- Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence -- Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach -- Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence -- Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence -- Senescence-Like Phenotypes in Human Nevi -- Detection of Oncogene-Induced Senescence In Vivo -- Detection of Senescence Markers During Mammalian Embryonic Development -- Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila
    Abstract: This book offers in a single volume a unique collection of the state-of-the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development
    Pages: XI, 220 p. 32 illus., 23 illus. in color. : online resource.
    ISBN: 9781493966707
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  • 24
    facet.materialart.
    Singapore : Springer
    Keywords: Medicine ; Gene Expression ; Human Physiology ; Endocrinology ; Andrology ; Cell Biology ; Biomedicine ; Human Physiology ; Cell Biology ; Endocrinology ; Andrology ; Gene Expression ; Springer eBooks
    Description / Table of Contents: Chapter 1. The Human Y Chromosome -- Chapter 2. Development of Gonads and Accessory Sex Glands -- Chapter 3. The Testis -- Chapter 4 -- Prostate -- Chapter 5. Seminal Vesicles -- Chapter 6. Genital Ducts & Other Accessory Sex Glands -- Chapter 7. Neurovascular Supply and Lymphatic Drainage of Male Reproductive Organs -- Chapter 8 -- Hypothalamo-Hypophyseal Testicular Axis -- Chapter 9. Testicular Hormones -- Chapter 10. Spermatogenesis. Chapter 11 -- The Human Semen. Chapter 12 -- The Basis of Sperm Function Tests -- Chapter 13 -- Male Puberty -- Chapter 14 -- Male Sexual Function. Chapter 15 -- Brain Areas Regulating Behavior -- Chapter 16. Neural Pathways of Behavior -- Chapter 17. Brain Sexual Dimorphism -- Chapter 18. Immunology of Male Reproductive System -- Chapter 19. Male Ageing and Andropause -- Chapter 20. Primary Testicular Failure -- Chapter 21. Biology of Male Hermaphrodite and Intersex -- Chapter 22. Thyroid and Male Reproduction -- Chapter 23. Metabolic Disorders and Male Reproduction -- Chapter 24. Gynecomastia -- Chapter 25. Male Contraception and Endocrine Disrupters
    Abstract: This book addresses various aspects of male reproduction ranging from mind to testis. The basis of maleness lies in the Y chromosome. Reproductive functions depend upon the development of male organs from embryo to manhood. Testis, the male gonad, produces hormones and sperms; the latter is ejaculated in semen secreted by accessory sex glands. The testicular events are under neuroendocrine regulation which coordinates reproductive life from puberty to andropause. Biology is as important as psychology in the control of reproduction. Behaviours are rooted in the brain. Various brain areas and neural circuits regulate male behaviours. Brain sexual polymorphism is the basis of homosexuality and transgenders. Neurophysiology has always been complex to understand. But, this book presents it in a simpler way. Reproductive organs receive systemic influences, too. The book describes roles of metabolic, immune and thyroid status in reproduction. The book has chapters on male reproductive pathophysiology. Principles of diagnosis and management are also included. The last section deals with contraception and yoga. The traditional wisdom of yoga has been used for millennia to enhance sexual and reproductive experience. This book will serve basic medical scientists, urologists, nephrologists, surgeons, andrologists, endocrinologists, gynaecologists, nurses, councellors and also the students of biological sciences who want to study reproduction in human male. The language is kept simple so that an inquisitive person with a background of biology too may read it
    Pages: XIX, 536 p. 160 illus., 110 illus. in color. : online resource.
    ISBN: 9789811036958
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  • 25
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems -- Genotyping DNA Variants with High Resolution Melting Analysis -- High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays -- In Situ Single Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification -- The MassARRAY® System for Targeted SNP Genotyping -- Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs) -- Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD -- Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT) -- Genotyping Complex Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification (MLPA) -- Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR -- Analysis Using Droplet Digital PCR -- Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII and Full-Length MT-DNA RSII -- Targeted Locus Amplification and Next Generation Sequencing -- Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ -- Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing -- Methods for Genotyping-by-Sequencing.-Describing Sequence Variants Using HGVS Nomenclature
    Abstract: This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping
    Pages: X, 254 p. 65 illus., 55 illus. in color. : online resource.
    ISBN: 9781493964420
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  • 26
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: High-Throughput Techniques for DNA Methylation Profiling -- Reduced Representation Bisulfite Sequencing (RRBS) and Cell Sorting Prior to DNA Methylation Analysis in Psychiatric Disorders -- Targeted DNA Methylation Analysis Methods -- Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes -- Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regulated by a-Ketoglutarate-Dependent Enzymes -- High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR -- Methods for MicroRNA Profiling in Cancer -- Microbiota and Epigenetic Regulation of Inflammatory Mediators -- Optical Microscopy and Spectroscopy for Epigenetic Modifications in Single Living Cells -- MicroRNAs in Therapy and Toxicity -- Genetics and Epigenetics of Multiple Sclerosis
    Abstract: This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicologyseries, the book includes the kind of detail and implementation advice to encourage success in the lab. Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics
    Pages: XII, 197 p. 38 illus., 22 illus. in color. : online resource.
    ISBN: 9781493967438
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  • 27
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction -- Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices -- Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates -- Monitoring Synapses Via Trans-Synaptic GFP Complementation -- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells -- Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins -- In Situ Protein Binding Assay Using Fc-Fusion Proteins -- ation Microscopy for the Investigation of Synaptic Structure and Function -- 3D dSTORM Imaging of Fixed Brain Tissue -- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses -- 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography -- Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles -- Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy -- Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors -- Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture -- Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ -- Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity -- Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging -- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus
    Abstract: This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit
    Pages: XI, 372 p. 79 illus., 67 illus. in color. : online resource.
    ISBN: 9781493966882
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  • 28
    Keywords: Medicine ; Biomedical Engineering ; Microbial ecology ; Bacteriology ; Microbiology ; Microbial genetics ; Microbial genomics ; Biomedicine ; Biomedical Engineering/Biotechnology ; Microbial Genetics and Genomics ; Eukaryotic Microbiology ; Microbial ecology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Part I: Next generation sequence technology -- 1.℗ Development of diverse sequencers machines for analysing host microbial communities -- 2.℗ Metagenomics, metatranscriptomics and metaproteomics tools for unveiling microbiome of hosts -- 3.℗ Challenges for analyzing large amount of data generated from diverse next generation sequence technology -- Part II: Plant microbiome -- 4.℗ Finding function of the plant phyllospheric microbial communities through metagenomics and metatranscriptomics analysis -- 5.℗ Metagenomics and metatranscriptomics studies of the plant rhizosphere -- 6.℗ Unraveling plant microbe interactions by using next generation sequencing technologies -- 7.℗ Modulation of microbiome in order to increase health and growth of the host -- 8.℗ Plant-bacterial interaction in heavy metal environmental -- 9.℗ The microbiome of the estero salado mangrove -- 10.℗ Metagenomics of endophytic microbial communities of plant -- Part III: Animal microbiome -- 11.℗ Advancements in molecular techniques and bioinformatics for understanding the rumen microbiome -- 12.℗ Ecology and evolution of the termite gut microbiota -- 13.℗ Microbial quality of insects -- 14.℗ Insect gut microbiome and its potential for biotechnological application -- 15.℗ Microbiome modulate behaviour of animal.-Part IV: Human microbiome. - 16.℗ Gut bacterial diversity of the tribes population of world -- 17.℗ Modulation of gut microbiome by different types of food habit -- 18.℗ Diversification of microbial communities of human skin and their role in defense -- 19.℗ Insight into microbiome of human milk via -omics technologies -- Part V: Marine microbiome. - 20.℗ Multi-omic approaches for mapping interactions among marine microbiomes
    Abstract: This book offers up-to-date information on different microbiomes, their community composition and interactive functions with the host, bringing together information from diverse research reports to provide an overview of the rapid developments in meta-omics technologies. It is a valuable resource for scientists, researchers, postgraduate and graduate students interested in understanding the impact and importance of next generation sequencing technologies on different hosts and their microbiomes
    Pages: XVI, 368 p. 45 illus., 35 illus. in color. : online resource.
    ISBN: 9789811050503
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  • 29
    Keywords: Medicine ; Human Physiology ; Molecular Biology ; Cardiology ; Biomedicine ; Molecular Medicine ; Human Physiology ; Cardiology ; Springer eBooks
    Description / Table of Contents: Section 1. Overview -- Section 2. Basic aspects of exosomes. -- Section 3. Exosomes as biomarkers of cardiovascular diseases. -- Section 4. Pathological Effects of Exosomes. -- Section 5. Therapeutic effects of Exosomes. -- Section 6. Future Prospects
    Abstract: The book provides and intensive overview on exosomes in cardiovascular diseases, its potential as biomarkers, as well as pathological and therapeutic effects. It firstly describes the general aspects of exosomes including the definition, formation and secretion of exosomes and highlight their roles as biomarkers and pathological and therapeutic effects in cardiovascular diseases as well. Secondly, basic aspects of exosomes including the purification methods of exosomes, exosomes content, and functional roles of the cardiovascular exosomes are summarized. Thirdly, exosomes as biomarkers of cardiovascular diseases are overviewed including their roles in diagnosis, prognosis and reaction to therapy. Fourthly, pathological effects of exosomes and therapeutic effects of exosomes are highlighted. Finally, future prospects of exosomes in cardiovascular research would be provided. This is an essential reference for researchers working in cell biology and regeneration, as well as clinicians such as cardiologist
    Pages: VII, 307 p. 32 illus., 30 illus. in color. : online resource.
    ISBN: 9789811043970
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  • 30
    Keywords: Medicine ; Immunology ; Pharmacology ; Internal Medicine ; Respiratory organs / Diseases ; Biomedicine ; Pharmacology/Toxicology ; Pneumology/Respiratory System ; Internal Medicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Part I Etiopathology and Genetics of Rare Lung Diseases -- Part II Treatment of Cystic Fibrosis -- Part III Treatment of Other Rare Lung Diseases
    Abstract: This volume describes the pathogenesis and pathophysiology of several pulmonary diseases as well as their treatment. It also discusses the underlying genetic and molecular biological basis, which opens the way for new treatments for these conditions. It focuses on the treatment of cystic fibrosis including CFTR (cystic fibrosis transmembrane-conductance regulator) modulator therapies, drug therapies that augment airway surface liquid as well as anti-inflammatory and anti-infective therapies. Further topics include long-term, low-dose macrolide therapy for diffuse panbronchiolitis; novel agents for previously untreatable idiopathic pulmonary fibrosis; possible new treatments for pulmonary alveolar proteinosis (PAP); and multiple novel therapeutic targets for treating lymphangiomyomatosis. Research into these conditions has led to major advances in our understanding of the underlying genetic and molecular basis of this disease, and to dramatic improvements in survival and quality of life for affected individuals
    Pages: VIII, 263 p. 33 illus., 14 illus. in color. : online resource.
    ISBN: 9783034809771
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  • 31
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models -- Hapten-specific T cell-mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate -- Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry -- Visualization of the T Cell Response in Contact Hypersensitivity -- Ultraviolet Radiation-induced Immunosuppression – Induction of Regulatory T cells -- Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model -- Xenotransplantation Model of Psoriasis -- A Mouse Model for Atopic Dermatitis using Topical Application of Vitamin D3 or of its Analog MC9039 Particle Bombardment of Ex-vivo Skin to Deliver DNA and Express Proteins -- Mouse Models of Asthma -- Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma -- Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse -- Induction and Analysis of Bronchus-associated Lymphoid Tissue -- Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes -- Isolation and Identification of Intestinal Myeloid Cells -- Monitoring and Modulation of Inducible Foxp3+ Regulatory T-cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon -- Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria -- Analysis of Leukocytes in Oral Mucosal Tissues -- Optimized Mouse Models for Liver Fibrosis -- Monitoring of Chemically Induced Colitis -- Oxazolone-induced Intestinal Inflammation in Adult Zebrafish -- High Dimensional Cytometry of Central Nervous System Leukocytes During Inflammation -- Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System -- Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools -- Quantitative Assessment of Cerebral Basement Membranes using Electron Microscopy -- Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish -- Experimental Arthritis Mouse Models Driven by Adaptive and/or Innate Inflammation -- Pain Relief in Nonhuman Primate Models of Arthritis -- Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis -- Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice
    Abstract: This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inflammation: Methods and Protocols aims to inspire the experienced investigator and the young experimenter alike to disentangle the fascinating process of inflammation
    Pages: XVI, 464 p. 110 illus., 82 illus. in color. : online resource.
    ISBN: 9781493967865
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  • 32
    Keywords: Medicine ; Human Physiology ; Cell Physiology ; Curriculums (Courses of study) ; Education / Curricula ; Medical Education ; Biomedicine ; Human Physiology ; Medical Education ; Cell Physiology ; Curriculum Studies ; Springer eBooks
    Description / Table of Contents: Reforming science education/reforming physiology education -- What is the new paradigm and what is new about it?- What are the core concepts of physiology?- What does it mean to €œúnpacḱ€ a core concept?- The €œúnpacked́€ core concept of homeostasis -- The €œúnpacked́€ core concept of flow down gradients -- The €œúnpacked́€ core concept of cell-cell communication -- Organizing an introductory physiology course based on core -- Teaching physiology using the new paradigm: three examples -- Using core concepts in physiology in designing learning resources -- Conceptual assessment of student learning -- Core concepts and the physiology curriculum -- Extending the paradigm -- Summing up
    Abstract: This book offers physiology teachers a new approach to teaching their subject that will lead to increased student understanding and retention of the most important ideas. By integrating the core concepts of physiology into individual courses and across the entire curriculum, it provides students with tools that will help them learn more easily and fully understand the physiology content they are asked to learn. The authors present examples of how the core concepts can be used to teach individual topics, design learning resources, assess student understanding, and structure a physiology curriculum
    Pages: XVI, 153 p. 9 illus., 4 illus. in color. : online resource.
    ISBN: 9781493969098
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  • 33
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Cancer Nanotechnology: Opportunities for Prevention, Diagnosis, and Therapy -- Improved Targeting of Cancers with Nanotherapeutics -- Multifunctional Liposomes -- Multifunctional Concentric FRET-Quantum Dot Probes for Tracking and Imaging of Proteolytic Activity -- Preparation and Characterization of Magnetic Nano-in-Microparticles for Pulmonary Delivery -- Multifunctionalization of Gold Nanoshells -- Fabrication of Photothermal Stable Gold Nanosphere/Mesoporous Silica Hybrid Nanoparticle Responsive to Near-Infrared Light -- Engineering Well-Characterized PEG-Coated Nanoparticles for Elucidating Biological Barriers to Drug Delivery -- Piloting Your Nanovehicle to Overcome Biological Barriers -- Detecting Sonolysis of Polyethylene Glycol Upon Functionalizing Carbon Nanotubes -- Methods for Generation and Detection of Non-Stationary Vapor Nanobubbles Around Plasmonic Nanoparticles -- Force Measurements for Cancer Cells -- Fractal Analysis of Cancer Cell Surface -- Quantitative Evaluation of the Enhanced Permeability and Retention (EPR) Effect -- Nanotechnology-Based Cancer Vaccines -- Designing Multicomponent Nanosystems for Rapid Detection of Circulating Tumor Cells -- Fluorescence and Bioluminescence Imaging of Orthotopic Brain Tumors in Mice -- An Ultrasensitive Biosensing Platform Employing Acetylcholinesterase and Gold Nanoparticles -- Gene Silencing Using Multifunctionalized Gold Nanoparticles for Cancer Therapy -- Generation of Dose Response Curves and Improved IC50’s for PARP Inhibitor Nanoformulations -- Artificial Antigen-Presenting Cells for Immunotherapies -- Exploiting Uptake of Nanoparticles by Phagocytes for Cancer Treatment -- Pulmonary Delivery of Magnetically-Targeted Nano-in-Microparticles -- Neutron-Activatable Nanoparticles for Intraperitoneal Radiation Therapy -- Nanoparticle-Mediated X-Ray Radiation Enhancement for Cancer Therapy -- Radiosensitizing Silica Nanoparticles Encapsulating Docetaxel for Treatment of Prostate Cancer
    Abstract: This detailed volume presents protocols for advancing the utility of nanotechnology in cancer research toward improving our understanding of cancer biology, prevention, diagnosis, and therapy. There are continuous new discoveries in the field of nanotechnology, thus creating new imaging systems or therapies, and this book focuses on how to employ certain discoveries for studying cancer by presenting principles along with techniques to allow for the transformation of any new discoveries in the field into cancer-studying tools with the hope of bringing in the involvement of biomedical scientists who can enhance the speed of discoveries toward cancer diagnosis and therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and motivating, Cancer Nanotechnology: Methods and Protocols serves as an ideal resource for biomedical scientists interested in the potential of this field as well as for physical scientists and engineers interested in employing nanotechnology in cancer diagnosis and therapy
    Pages: XIV, 414 p. 100 illus., 77 illus. in color. : online resource.
    ISBN: 9781493966462
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  • 34
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation -- Basic Aspects of T Helper Cell Differentiation -- FACS Analysis of Memory T lymphocytes -- Intravital Microscopy Analysis of Hepatic T cell dynamics -- Analysis of T Cell Activation by Confocal Microscopy -- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion -- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel -- Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors -- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer -- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification -- Strategies for T Helper Cell Subset Differentiation from Naïve Precursors -- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells -- Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation -- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation -- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR -- Single-cell RNA Sequencing of Human T Cells -- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry -- Developmental and Functional Assays to Study Murine and Human gd T Cells
    Abstract: This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails, approaches to measure telomerase activity in terminally differentiated T cells, and approaches to define Treg cells at the phenotypic and functional level. The final part of the book is dedicated to the analysis of the differentiation and effector functions of innate T cells, namely the well-known γ/δ T cells, and the recently identified CD8+ mucosal associated invariant T (MAIT) cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Differentiation: Methods and Protocols aims to provide protocols that are fundamental to monitor the T cell compartment at the level of single cells in pathological and immunotherapy conditions
    Pages: XI, 271 p. 55 illus., 44 illus. in color. : online resource.
    ISBN: 9781493965489
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  • 35
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Overview of Vaccine Adjuvants: Introduction, History, and Current Status -- Development of the CpG Adjuvant 1018: A Case Study -- Syntheses of Human TLR8-Specific Small-Molecule Agonists -- Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21 -- QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes -- Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography -- Biosynthetic Approaches to Squalene Production: The Case of Yeast -- In Silico Adjuvant Design and Validation -- Manufacturing Methods for Liposome Adjuvants -- Synthesis of Lymph Node-Targeting Adjuvants -- Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination -- Manufacture of Oil-in-Water Emulsion Adjuvants -- Methods to Prepare Aluminum Salt-Adjuvanted Vaccines -- Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines -- Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product -- Stressed Stability Techniques for Adjuvant Formulations -- Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) -- Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling -- Determination of Protein Content in Alhydrogelâ-Based Vaccines by O-Phthalaldehyde Assay -- Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference -- Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques -- Screening Vaccine Formulations in Fresh Human Whole Blood -- Analysis of the Innate Response to Adjuvants: Characterization of the Draining Lymph Node by Fluorescence-Activated Cell Sorting -- Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants -- Eliciting Epitope-Specific CD8 T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols -- Molecular Methods and Bioinformatic Tools for Adjuvant Characterization by High-Throughput Sequencing
    Abstract: This collection seeks to elucidate the practical methods necessary for successful adjuvant development, with a particular focus on the synthesis, formulation, manufacturing, and characterization aspects involved. Beginning with an overview and a case study, the book then delves into in silico design, chemical synthesis, biosynthesis, and/or purification from natural raw materials of specific adjuvant molecules, adjuvant formulation approaches, the analytical characterization of adjuvant formulations and adjuvant-containing vaccines, as well as the biological characterization of vaccine adjuvant activity, including in vitro and in vivo approaches, to measure innate and adaptive immune responses. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Vaccine Adjuvants: Methods and Protocols aims to facilitate vaccine adjuvant product development efforts, making them more accessible, manufacturable, and better characterized
    Pages: XII, 372 p. 75 illus., 33 illus. in color. : online resource.
    ISBN: 9781493964451
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  • 36
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    New York, NY : Springer
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Principles of Stereotaxy in Small Animals -- EEG Indices of Cortical Network Formation and their Relevance for Studying Variance in Subjective Experience and Behavior -- Pharmaco-Based fMRI and Neurophysiology in Non-Human Primates -- Optogenetic Manipulations of Neuronal Network Oscillations: Combination of Optogenetics and Electrophysiological Recordings in Behaving Mice -- 3D-Video-Based Computerized Behavioral Analysis for In Vivo Neuropharmacology and Neurophysiology in Rodents -- Operant Self-Administration of Chocolate in Rats: An Addiction-Like Behavior -- Electrical Nerve Stimulation and Central Microstimulation -- In Vivo Biosensor Based on Prussian Blue for Brain Chemistry Monitoring: Methodological Review and Biological Applications -- Monitoring Extracellular Molecules in Neuroscience by In Vivo Electrochemistry: Methodological Considerations and Biological Applications -- Push-Pull Superfusion: A Technique for Investigating Involvement of Neurotransmitters in Brain Function -- Involvement of Neurotransmitters in Mnemonic Processes, Response to Noxious Stimuli and Conditioned Fear: A Push-Pull Superfusion Study -- Neurophysiological Approaches for In Vivo Neuropharmacology -- Involvement of Neurotransmitters in Behavior and Blood Pressure Control -- Functional Mapping of Somatostatin Receptors in Brain: In Vivo Microdialysis Studies -- The Impact of Cannabinoids on Motor Activity and Neurochemical Correlates -- Modeling Schizophrenia: Focus on Developmental Models -- Immunohistochemical Analysis of Fos Protein Expression for Exploring Brain Regions Related to Central Nervous System Disorders and Drug Actions -- Involvement of Nitric Oxide in Neurotoxicity Produced by Psychostimulant Drugs
    Abstract: This volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Cutting-edge and practical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders
    Pages: XIII, 428 p. 142 illus., 69 illus. in color. : online resource.
    ISBN: 9781493964901
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  • 37
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development -- The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients -- Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research -- LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics -- Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies -- Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations -- Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective -- Multiplex Analyses Using Real-Time Quantitative PCR -- Multiplex Analysis Using cDNA Transcriptomic Profiling -- Multiplex Single Nucleotide Polymorphism Analyses -- Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture -- Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies -- Multiplex Immunoassay Profiling -- Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets -- Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets -- Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders -- Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes -- Selective Reaction Monitoring for Quantitation of Cellular Proteins -- Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry -- Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses -- Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS -- UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life -- iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification -- 1H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples -- Lab-on-a-Chip Multiplex Assays -- Multiplex Smartphone Diagnostics -- Development of a User-Friendly App for Assisting Anticoagulation Treatment -- Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine
    Abstract: This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 〈 Authoritative and invaluable, Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques
    Pages: XV, 317 p. 59 illus., 48 illus. in color. : online resource.
    ISBN: 9781493967308
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  • 38
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Current Challenges in the Diagnosis of Fungal Infections -- The Changing Epidemiology of Invasive Fungal Infections -- Current Algorithms in Fungal Diagnosis in the Immunocompromised Host -- Commercial Molecular Tests for Fungal Diagnosis from a Practical Point of View -- Systemic Antifungal Agents: Current Status and Projected Future Developments -- Fungal-Grade Reagents and Materials for Molecular Analysis -- Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases -- Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue -- Histopathology -- Culture-Based Techniques -- Serological Approaches -- Isolation of Nucleic Acids for Fungal Diagnosis -- Prerequisites for Control of Contamination in Fungal Diagnosis -- Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques -- Genus- and Species-Specific PCR Detection Methods -- Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization -- Nuclear Magnetic Resonance Spectroscopy-Based Identification of Yeast -- T2 Magnetic Resonance for Fungal Diagnosis -- Fungal Species Identification by MALDI-ToF Mass Spectrometry -- Immunological Identification of Fungal Species -- The Molecular Blueprint of a Fungus by Next-Generation Sequencing (NGS) -- Microarray Technologies in Fungal Diagnostics -- Molecular Detection of Resistance to Echinocandins -- Molecular Detection of Resistance to Azole Components -- Immune Cell-Supplemented Human Skin Model for Studying Fungal Infections
    Abstract: This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The knowledge of host-related predisposing factors and stratified treatment options facilitating timely onset of adequate antifungal therapy are critical for successful clinical management and outcome of invasive fungal disease (IFD), requiring not only rapid diagnosis of a fungal infection and identification of the causative species, but also assessment of pathogen/host factors related to pathogenicity, susceptibility, and response to treatment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Human Fungal Pathogen Identification: Methods and Protocols serves as an ideal reference for researchers investigating the ever-growing worldwide healthcare problems involving fungal infections
    Pages: XI, 453 p. 66 illus., 49 illus. in color. : online resource.
    ISBN: 9781493965151
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  • 39
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Salivary Diagnostics using Purified Nucleic Acids -- RNA Sequencing Analysis of Salivary Extracellular RNA -- Qualitative and Quantitative Proteomic Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry -- Antioxidant Micronutrients and Oxidative Stress Biomarkers -- NMR-Based Metabolomics of Oral Biofluids -- Gene Therapy of Salivary Diseases -- The Oral Microbiota in Health and Disease: An Overview of Molecular Findings -- Microbial Community Profiling using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) -- Analysis of 16S rRNA Gene Amplicon Sequences using the QIIME Software Package -- Adhesion of Yeast and Bacteria to Oral Surfaces -- Quantitative Analysis of Periodontal Pathogens by Real-Time Polymerase Chain Reaction -- Methods to Study Antagonistic Activities Among Oral Bacteria -- Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones -- Markerless Genome Editing in Competent Streptococci -- Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns -- Generating Multiple Base-Resolution DNA Methylomes using Reduced Representation Bisulfite Sequencing -- A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands -- Genome-Wide Expression Analysis of Periodontal Cells and Tissues -- Differential Expression and Functional Analysis of High-Throughput -Omics Data Using Open Source Tools -- Exploring Genome-Wide Expression Profiles Using Machine Learning Techniques -- Embryonic Explant Culture: Studying Effects of Regulatory Molecules on Gene Expression in Craniofacial Tissues -- Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease -- Fabrication and Characterization of Decullularized Periodontal Ligament Cell Sheet Constructs -- A Method to Isolate, Purify and Characterize Human Periodontal Ligament Stem Cells -- Constructing Tissue Microarrays: Protocols and Methods Considering Potential Advantages and Disadvantages for Downstream Use -- Growing Adipose-Derived Stem Cells under Serum-Free Conditions -- Quantitative Real-Time Gene profiling of Human Alveolar Osteoblasts -- Proteomic Analysis of Dental Tissue Microsamples -- Characterization, Quantification and Visualization of Neutrophil Extracellular Traps
    Abstract: This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases
    Pages: XIV, 503 p. 92 illus., 54 illus. in color. : online resource.
    Edition: 2nd ed. 2017.
    ISBN: 9781493966851
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  • 40
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Introduction to RNA Vaccines -- Self-replicating RNA -- Self-replicating RNA Vaccine Delivery to Dendritic Cells -- Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons -- RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines -- Nucleoside Modified mRNA Vaccines for Infectious Diseases -- Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy -- Measuring the Adjuvant Activity of RNA Vaccines -- Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles -- Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins -- Adjuvant-Enhanced mRNA Vaccines -- Enhanced Delivery of DNA or RNA Vaccines by Electroporation -- The European Regulatory Environment of RNA-Based Vaccines -- Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome -- Considerations for Producing mRNA Vaccines for Clinical Trials -- Nonclinical Safety Testing of RNA Vaccines -- Immunotherapy of Uveal Melanoma: Vaccination Against Cancer
    Abstract: This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today’s challenges
    Pages: XI, 282 p. 42 illus., 26 illus. in color. : online resource.
    ISBN: 9781493964819
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  • 41
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Cerebrotendinous Xanthomatosis Presenting with Infantile Spasms and Intellectual Disability -- Hyperammonemia as a Presenting Feature in Two Siblings with℗ FBXL4℗ Variants -- Intracranial Hypertension in Cystinosis Is a Challenge: Experience in a Childreń€™s Hospital -- Severe Respiratory Acidosis in Status Epilepticus as a Possible Etiology of Sudden Death in Lesch́€“Nyhan Disease: A Case Report and Review of the Literature -- Vitamin B12℗ Administration by Subcutaneous Catheter Device in a Cobalamin A (cblA) Patient -- Expansion of the Phenotypic Spectrum of Propionic Acidemia with Isolated Elevated Propionylcarnitine -- Previously Unreported Biallelic Mutation in℗ DNAJC19:Are Sensorineural Hearing Loss and Basal Ganglia Lesions Additional Features of Dilated Cardiomyopathy and Ataxia (DCMA) Syndrome? -- Lysosomal Storage Disorders in Nonimmune Hydrops Fetalis (NIHF): An Indian Experience -- The Risk of Fatty Acid Oxidation Disorders and Organic Acidemias in Children with Normal Newborn Screening -- Clinical and Mutational Characterizations of Ten Indian Patients with Beta-Ketothiolase Deficiency -- Atypical Presentation and Treatment Response in a Child with Familial Hypercholesterolemia Having a Novel LDLR Mutation -- Development of a Tandem Mass Spectrometry Method for Rapid Measurement of Medium- and Very-Long-Chain Acyl-CoA Dehydrogenase Activity in Fibroblasts -- Analysis of Melanin-like Pigment Synthesized from Homogentisic Acid, with or without Tyrosine, and Its Implications in Alkaptonuria -- Bone Health in Classic Galactosemia: Systematic Review and Meta-Analysis -- Cognitive Development in a Young Child with Mucolipidosis Type IV: A Case Report -- White Matter Microstructure and Subcortical Gray Matter Structure Volumes in Aspartylglucosaminuria; a 5-Year Follow-up Brain MRI Study of an Adolescent with Aspartylglucosaminuria and His Healthy Twin Brother -- Erratum to: White Matter Microstructure and Subcortical Gray Matter Structure Volumes in Aspartylglucosaminuria; a 5-Year Follow-up Brain MRI Study of an Adolescent with Aspartylglucosaminuria and His Healthy Twin Brother
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 117 p. 20 illus., 10 illus. in color. : online resource.
    ISBN: 9783662558331
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  • 42
    Keywords: Medicine ; Immunology ; Molecular Biology ; Posttranslational Modification ; Cell Biology ; Biomedicine ; Immunology ; Cell Biology ; Posttranslational Modification ; Molecular Medicine ; Springer eBooks
    Abstract: This book discusses recent research in innate immunity, which has revealed a large number of receptors that sense the presence of microorganisms or cellular damage in tissues. In complex tissues, many of these sensing events occur simultaneously. Thus, the downstream signaling pathways need to be integrated so that an appropriate cellular inflammatory response can be initiated. In addition, the inflammasome defines the molecular and cellular processes of inflammation in response to microbial infection.℗ Previous data suggested that regulation of inflammasomes is mediated by microbes, but inflammasomes also have antimicrobial functions. Increasing evidence in mouse models, together with human data, strongly implicates an involvement of the inflammasome and uncontrolled inflammation in the initiation and progression of diseases with a high impact on public health.℗ The book reviews novel aspects of functional genomics, epigenomics, transcriptomics, post-translat ional modifications, microbiome and immunometabolism in order to understand inflammatory signaling and responses, covering recent findings on the mechanisms underlying the regulation of inflammatory responses to pathogens, dysregulation of these responses in inflammatory disease, and the use of such mechanisms to boost or subdue the inflammatory response. Bridging the gaps in understanding between the fields of human and mouse immunology, it provides valuable insights into inflammatory-mediated disease and immune defense. Such innovative perspectives in both basic and clinical research promote the translation of knowledge to the clinic
    Pages: XII, 244 p. 19 illus., 18 illus. in color. : online resource.
    ISBN: 9789811059872
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  • 43
    Keywords: Medicine ; Medical genetics ; Biomedical Engineering ; Nucleic Acids ; Proteins ; Biomedicine ; Biomedical Engineering/Biotechnology ; Biological and Medical Physics, Biophysics ; Nucleic Acid Chemistry ; Protein Science ; Gene Function ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Principles of protein structure and function -- Protein folding -- Nucleic acid structure and functions -- Higher order nucleic acid structures -- Molecular Interactions -- Lipid and membrane structures -- Chapter 7. Membrane biophysics -- Techniques in Biophysics -- Advance techniques in Biophysics
    Abstract: This comprehensive book presents a modern concept in biophysics based on recently published research. It highlights various aspects of the biophysical fundamentals and techniques that are currently used to study different physical properties of biomolecules, and relates the biological phenomenon with the underlying physical concepts. The content is divided into nine chapters summarizing the structural details of proteins, including recently discovered novel folds, higher order structures of nucleic acids, as well as lipids and the physical forces governing the macromolecular interactions which are essential for the various biological processes. It also provides insights into recent advances in biophysical techniques including Hydrogen Deuterium Exchange with Mass Spectrometry (HDX-MS), Small Angle X-ray Scattering (SAXS) and cryo Electron Microscopy (cryo EM), supplemented with interesting experimental data. It is a valuable reference resource for anyone with a desire to gain a better understanding of the fundamentals of biophysical concepts and techniques used to study important biomolecules
    Pages: XVII, 274 p. 133 illus., 78 illus. in color. : online resource.
    ISBN: 9789811049682
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  • 44
    Keywords: Medicine ; Cancer Research ; Human Physiology ; Molecular Biology ; Pharmaceutical technology ; Biomedicine ; Human Physiology ; Molecular Medicine ; Pharmaceutical Sciences/Technology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Physiological and Pathological Functions of Mitochondrial Proteases -- The Role of MMP2 and 9 in Embryonic Neural Crest Cells and their Derivatives -- The matrix metalloproteinase and tissue inhibitors of metalloproteinase balance in physiologicaland pathological remodeling of skeletal muscles -- Role of BMP1/ tolloid like proteases in bone morphogenesis and tissue remodeling -- Role of proteases in the regulation of N-myristoyltransferase -- Role of Tissue Factor-FVIIa Blood Coagulation Initiation complex in Cancer -- Metalloproteases in adaptive cell responses -- Proteases from protozoa and℗ their role in infection.-Regulation of Extracellular Matrix Remodeling and℗ Epithelial-Mesenchymal Transition by Matrix Metalloproteinases: Decisive Candidates in Tumor Progression.-℗ Proteases and Protease Inhibitors in Male Reproduction -- Physiological and Pathological Functions of℗ Cysteine Cathepsins -- Role of serine proteases and inhibitors in cancer -- Role of Proteases in diabetes and diabetic complications.-℗ Plant Latex Proteases: Natural Wound Healers -- Emerging Role of Mitochondrial Serine Protease HtrA2 in Neurodegeneration -- Functional Relevance of Deubiquitinases in Life and Disease -- Sub-Mitochondrial Calpains in Pathophysiological Consequences.-℗ Serine Proteases in the Lectin Pathway of the Complement System.-℗ Pups, SAMPs and Prokaryotic Proteasomes.-℗ Role of Proteases in Photoaging of Skin.-℗ Insect proteases: Structural-functional outlook.-℗ Protease-antiprotease interactions:℗ an overview of the process from an℗ in silico℗ perspective -- Snake venom proteases as toxins and tools.-℗ The world of the proteases across micros, insects and medicinal trees.-℗ Insight into the mode of interactions between bacterial proteases and their substrates.-℗ The Ubiquitin Proteasome System with itsChecks and Balances.-℗ A brief account of structure function relationship of the traditional cysteine protease inhibitor-cystatin with a special focus on human family 1 & 2 cystatins.-℗ Solid Support Synthesis of a Dnp-labeled Peptide for Assay of Matrix Metalloproteinase-2
    Abstract: Using a multidisciplinary approach, this book describes the biochemical mechanisms associated with dysregulation of proteases and the resulting pathophysiological consequences. It highlights the role and regulation of different types of proteases as well as their synthetic and endogenous inhibitors. The role of proteases was initially thought to be limited to general metabolic digestion. However, we now know that the role of protein breakdown is much more complex, and proteases have multiple functions: they are coupled to turnover and can affect protein composition, function and synthesis. In addition to eliminating abnormal proteins, breakdown has many modulatory functions, including activating and inactivating enzymes, modulating membrane function, altering receptor channel properties, affecting transcription and cell cycles and forming active peptides. The ubiquity of proteases in nature makes them an important target for drug development. This in-depth, comprehensive is a valuable resource for researchers involved in identifying new targets for drug development. With its multidisciplinary scope, it bridges the gap between fundamental and translational research in the biomedical and pharmaceutical industries, making it thought-provoking reading for scientists in the field
    Pages: XV, 619 p. 97 illus., 78 illus. in color. : online resource.
    ISBN: 9789811025136
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  • 45
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: False-Positive Newborn Screen Using the Beutler Spot Assay for Galactosemia in Glucose-6-Phosphate Dehydrogenase Deficiency -- Domains of Daily Physical Activity in Children with Mitochondrial Disease: A 3D Accelerometry Approach -- Preliminary Results on Long-Term Potentiation-Like Cortical Plasticity and Cholinergic Dysfunction After Miglustat Treatment in Niemann-Pick Disease Type C -- Prevalence of Mucopolysaccharidosis Types I, II, and VI in the Pediatric and Adult Population with Carpal Tunnel Syndrome (CTS). Retrospective and Prospective Analysis of Patients Treated for CTS -- Primary Carnitine Deficiency: Is Foetal Development Affected and Can Newborn Screening Be Improved? -- The Spectrum of Niemann-Pick Type C Disease in Greece -- What Is the Best Blood Sampling Time for Metabolic Control of Phenylalanine and Tyrosine Concentrations in Tyrosinemia Type 1 Patients? -- A Homozygous Mutation in GPT2 Associated with Nonsyndromic Intellectual Disability in a Consanguineous Family from Costa Rica -- Impact of Dietary Intake on Bone Turnover in Patients with Phenylalanine Hydroxylase Deficiency -- Hypogonadotropic Hypogonadism in Males with Glycogen Storage Disease Type 1 -- Widespread Expression of a Membrane-Tethered Version of the Soluble Lysosomal Enzyme Palmitoyl Protein Thioesterase-1 -- Gamma-Hydroxybutyrate (GHB) Content in Hair Samples Correlates Negatively with Age in Succinic Semialdehyde Dehydrogenase Deficiency -- An Audit of the Use of Gonadorelin Analogues to Prevent Recurrent Acute Symptoms in Patients with Acute Porphyria in the United Kingdom -- Altered Cellular Homeostasis in Murine MPS I Fibroblasts: Evidence of Cell-Specific Physiopathology -- Irreversibility of Symptoms with Biotin Therapy in an Adult with Profound Biotinidase Deficiency
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 120 p. 20 illus., 11 illus. in color. : online resource.
    ISBN: 9783662561386
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  • 46
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Large Scale Transcriptome Analysis -- Methods to Assess Genetic Risk Prediction -- Microarray Analysis of Hypertension -- Tissue Proteomics in Vascular Disease -- Urine Metabolomics in Hypertension Research -- Systems Biology Approach in Hypertension Research -- Measurement of Angiotensin Peptides: HPLC, RIA -- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2) -- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate -- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays -- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes -- Dopaminergic Immunofluorescence Studies in Kidney Tissue -- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps) -- Intracellular Free Calcium Measurement Using Confocal Imaging -- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells -- In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs -- In vitro and in vivo Approaches to Assess Rho-kinase Activity -- NADPH Oxidases and Measurement of Reactive Oxygen Species -- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation -- Assessment of Caveolae/Lipid Rafts in Isolated Cells -- Isolation and Characterization of Circulating Microparticles by Flow Cytometry -- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR -- Isolation and Differentiation of Murine Macrophages -- Isolation and Differentiation of Human Macrophages -- Isolation of Immune Cells for Adoptive Transfer -- Isolation and Culture of Endothelial Cells from Large Vessels -- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels -- Evaluation of Endothelial Dysfunction in vivo -- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions -- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of〈 PPARγ -- Renal Delivery of Anti-microRNA Oligonucleotides in Rats -- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs
    Abstract: This volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on genetics and mmics of hypertension; the Renin-Angiotensin-Aldosterone System; vasoactive agents and hypertension; signal transduction and reactive oxygen species; novel cell models and approaches to study molecular mechanisms of hypertension; vascular physiology; and new approaches to manipulate mouse models to study molecular mechanisms of hypertension. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hypertension: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XV, 437 p. 78 illus., 40 illus. in color. : online resource.
    ISBN: 9781493966257
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  • 47
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Haplotyping of Heterozygous SNPs in Genomic DNA using Long Range PCR -- Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA -- Pacbio for Haplotyping in Gene Families- High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes -- High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture -- Pedigree-defined Haplotypes and their Applications to Genetic Studies -- Haplotyping a Non-Meiotic Diploid Fungal Pathogen using Induced Aneuploidies and SNP/CGH Microarray Analysis -- Whole-genome Haplotyping of Single Sperm of Daphnia Pulex (Crustacea, Anomopoda) -- Chromosome-range Whole-genome High-throughput Experimental Haplotyping by single-chromosome Microdissection -- Phased Genome Sequencing through Chromosome Sorting -- Long Fragment Read (LFR) Technology: Cost-effective, High-quality Genome-wide Molecular Haplotyping -- Contiguity-Preserving Transposition Sequencing (CPT-seq) for Genome-Wide Haplotyping, Assembly, and Single-Cell ATAC-seq -- Fosmid Pool-Based Next Generation Sequencing to Haplotype-Resolve Whole Genomes -- Discovery of Rare-haplotype Variants by Typing Millions of Single-molecules with Bead Emulsion Haplotyping (BEH) -- Computational Haplotype Inference from Pooled Samples
    Abstract: This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes. Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted loci, and long-range haplotyping for whole chromosomes or genomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Haplotyping: Methods and Protocols, aims to provide researchers with an overview of experimental methods for haplotyping
    Pages: X, 326 p. 54 illus., 37 illus. in color. : online resource.
    ISBN: 9781493967506
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  • 48
    Keywords: Medicine ; Drug Resistance ; Biomedicine ; Drug Resistance ; Springer eBooks
    Description / Table of Contents: Bacteria -- Origins of antibiotic resistance (Resistome) -- Dissemination of antibiotic resistance -- Mechanisms of antibiotic resistance.-Strategies for combating antibiotic resistance -- Multiple-drug-resistant bacteria -- Resistant Tuberculosis -- MRSA -- Fungi -- Microevolution of drug resistance -- Environmental induction of azole resistance in medical molds -- Echinocandin resistance in medically relevant fungi -- Role of drug efflux in azole resistance -- Parasites -- Drug-resistant Leishmania -- Drug resistance in Helminth infections -- Drug resistance in Malaria -- Drug resistance in schistosome infections -- Drug resistance in trypanosome infections -- HIV -- Resistance to RT inhibitors -- Resistance to Integrase Inhibitors -- Resistance to Entry inhibitors -- Resistance Testing -- Virology -- Resistance to viral protease inhibitors: The substrate envelope -- Resistance to new Direct-Acting-Antivirals against HCV -- HBV Resistance -- Resistance to antiherpetic drugs -- Influenza Resistance
    Abstract: This is a comprehensive tool covering all manifestations of antimicrobial resistance, with viral, bacterial, parasitical and fungal resistance each given a dedicated section. The underlining molecular mechanisms, which depend not only on the microbe, but on the specific drug (target), are highly diverse, and are covered in great detail. This work also discusses and compares the biological, biochemical and structural aspects of resistance and its evolution
    Pages: 73 illus., 42 illus. in color. eReference. : online resource.
    ISBN: 9781493906949
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  • 49
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells -- Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9 -- Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice -- All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells -- CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium -- Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii -- Generation of Stable Knockout Mammalian Cells By TALEN-Mediated Locus-Specific Gene Editing -- Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell- Mediated Semi-Cloned Technology. - Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes -- Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells -- Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis -- in silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships -- in silico Prediction of Deleteriousness for Non-Synonymous and Splice-Altering Single Nucleotide Variants in the Human Genome -- in silico methods for Analyzing Mutagenesis Targets -- Methods for Detecting Critical Residues in Proteins -- A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants -- Application of in vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea -- Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors -- PERMutation Using Transposase Engineering (PERMUTE): a Simple Approach for Constructing Circularly-Permuted Protein Libraries -- Transposon Insertion Mutagenesis for Archaeal Gene Discovery -- Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis -- Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method -- Seamless Ligation Cloning Extract (Slice) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to Slip Site-Directed Mutagenesis -- Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly -- Revised Mechanism and Improved Efficiency of the Quikchange Site-Directed Mutagenesis Method -- An in vitro Single Primer Site-Directed Mutagenesis Method for Use in Biotechnology -- Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Site-Specifically Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function -- Step-by-Step in vitro Mutagenesis - Lessons from Fucose-Binding Lectin PA-IIL -- Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein-Cofactor and Protein-Protein Functional Relationships -- Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions -- Use of Random and Site-Directed Mutagenesis to Probe Protein Structure-Function Relationships: Applied Techniques in the Study of Helicobacter pylori -- Novel Random Mutagenesis Method for Directed Evolution -- Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent -- Development and Use of a Novel Random Mutagenesis Method: in Situ Error-Prone PCR (is-epPCR)
    Abstract: In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering
    Pages: XV, 511 p. 96 illus., 52 illus. in color. : online resource.
    ISBN: 9781493964727
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  • 50
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Metabolic Diseases ; Pediatrics ; Biomedicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Difficulties in Daily Life and Associated Factors, and QoL of Children with Inherited Metabolic Disease and Their Parents in Japan: A Literature Review -- Swallow Prognosis and Follow-Up Protocol in Infantile Onset Pompe Disease -- Clinical and Genetic Characteristics of Romanian Patients with Mucopolysaccharidosis Type II -- Gastrointestinal Health in Classic Galactosemia -- Management of Life-Threatening Tracheal Stenosis and Tracheomalacia in Patients with Mucopolysaccharidoses -- Brain White Matter Integrity Mediates the Relationship Between Phenylalanine Control and Executive Abilities in Children with Phenylketonuria -- The Challenges of a Successful Pregnancy in a Patient with Adult Refsum’s Disease due to Phytanoyl-CoA Hydroxylase Deficiency -- Novel Homozygous Missense Mutation in SPG20 Gene Results in Troyer Syndrome Associated with Mitochondrial Cytochrome c Oxidase Deficiency -- Lethal Neonatal LTBL Associated with Biallelic EARS2 Variants: Case Report and Review of the Reported Neuroradiological Features -- Leukoencephalopathy due to Complex II Deficiency and Bi-Allelic SDHB Mutations: Further Cases and Implications for Genetic Counselling -- Peak Jump Power Reflects the Degree of Ambulatory Ability in Patients with Mitochondrial and Other Rare Diseases -- R