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  • Books  (2,946)
  • DKFZ Publication Database  (6,478)
  • Germany  (4,803)
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  • 1
    Call number: M019:54
    Keywords: Pharmaceutical Preparations ; Germany
    Notes: Auch online verfügbar über die Homepage der Zentralbibliothek.
    Pages: various pagings
    Edition: 59. Ausg.
    ISBN: 9783946057420
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  • 2
    Call number: C100:54
    Keywords: Drug Utilization / trends ; Germany
    Pages: xv, 1134 p. : ill.
    ISBN: 9783662590454
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  • 3
    Unknown
    München : Zuckschwerdt
    Call number: QZ203:109 ; C097:001
    Keywords: Neoplasms / epidemiology ; Registries ; Germany ; epidemiology
    Pages: xiv, 219 p.
    ISBN: 9783863711658
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  • 4
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics -- MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential -- Involvement of miRNAs and Pseudogenes in Cancer -- microRNAs Reprogram Tumor Immune Response -- APOBEC Gene Expression Regulated by microRNAs -- MicroRNAs Changes the Landscape of Cancer Resistance -- MicroRNA, Noise, and Gene Expression Regulation -- Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer -- Detection of Plasma microRNA Signature in Osteosarcoma Patients -- Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells -- Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy -- Evaluation of microRNA Delivery in vivo -- Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer -- Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data -- Construction of Multi-potent microRNA Sponge and its Functional Evaluation -- microRNA Sequencing Data Analysis Toolkits
    Abstract: This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition℗ aims to ensure successful results in the further study of this vital field
    Pages: XI, 222 p. 21 illus., 17 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493974351
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  • 5
    Unknown
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.℗
    Pages: XII, 302 p. 66 illus., 63 illus. in color. : online resource.
    ISBN: 9781493974177
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  • 6
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Abstract: This volume presents a broad compendium of techniques used in biodegradation and toxicological research. Through both legacy and up-to-date approaches to practical methodologies with proven results, the book examines the role and applications of analytical biodegradation quantification as it applies to the environmental sciences, particularly in the range of byproducts that are usually linked to toxicology, and the test organisms most often used in toxicity testing. Topics include scientific and technical feasibility studies, contaminant impacts evaluation, study design and analytical techniques, key methodologies required to prepare the biodegradation and toxicology protocols, as well as the handling of microbial communities related to such processes. Written for the℗ Methods in Pharmacology and Toxicology℗ series, chapters deal with a critical discussion of laboratory scale experiments, as well as full scale in situ and ex situ apparatus, with each chapter containing both a discursive section along with a detailed methods section. Detailed and authoritative,℗ Toxicity and Biodegradation Testing℗ is primarily focused toward the environmental sciences researcher, while the range of techniques also provides an introduction to biodegradation and toxicology methods for researchers outside of the field
    Pages: XI, 397 p. 90 illus., 26 illus. in color. : online resource.
    ISBN: 9781493974252
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  • 7
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate in vivo Translatome -- Measuring Nascent Transcripts by Nascent-seq -- Genome-wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis -- Multiplexed Targeted Sequencing for Oxford Nanopore MinION; A Detailed Library Preparation Procedure -- Hi-Plex for Simple, Accurate, and Cost-effective Amplicon-based Targeted DNA Sequencing -- ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras -- Genome-wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach -- Sequencing of Genomes from Environmental Single Cells -- SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms -- CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers -- Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities -- A Bloody Primer Analysis of RNA-Seq from Tissue Admixtures -- Next-Generation Sequencing of Genome-wide CRISPR Screens -- Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells -- Investigate Global Chromosomal Interaction by Hi-C in Human Na©¯ve CD4 T Cells -- Primer Extension, Capture, and On-bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites.℗
    Abstract: This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,℗ method called ́€œHi-Plex́€ to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ Next Generation Sequencing: Methods and Protocols℗ aims to be useful and informative for further study into this vital field.℗
    Pages: XIII, 266 p. 51 illus., 41 illus. in color. : online resource.
    ISBN: 9781493975143
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  • 8
    Keywords: Medicine ; Pharmacology ; Medicinal Chemistry ; Ecotoxicology ; Biomedicine ; Pharmacology/Toxicology ; Ecotoxicology ; Medicinal Chemistry ; Springer eBooks
    Description / Table of Contents: Part 1.Introduction -- Introduction -- Part 2.Physical and Chemical Characteristics -- Physical Parameters of PAHs/NPAHs/Oxidative Derivatives -- Chemistry of PAHs/NPAHs/Oxidative Derivatives (structures/name/numbering by IUPAC) -- Part 3.Analytical Method -- PAHs/NPAHs (including Application to Environmental Samples) -- PAHOHs/PAHQs (including application to biological samples) -- Part 4 Atmospheric Polycyclic Aromatic Hydrocarbons and PM2.5 -- Sampling Methods for PM -- PAHs/NPAHs in PM (including formation of PAHs/NPAHs) -- Atmospheric Reaction: Formation and Degradation -- PAHs/NPAHs in Japan (Tokyo/Sagamihara, Hokkaido, Kanazawa, Kitakyushu, Muroran) -- PAHs/NPAHs in North-East Asia (China, Korea, Russia) -- Long-Range Transport of PAHs/NPAHs (Noto and Tateyakma) -- PAHs/NPAHs in South East Asia -- PAHs/NPAHs in Africa -- Emission Sources of PAHs/NPAHs (including source marker) -- Emission of PAHs/NPAHs from Diesel-Engine Vehicles -- Model Simulation of PAHs in North-East Asia -- Part 5.Polycyclic Aromatic Hydrocarbons in Marine -- PAHs Pollution caused by Oil Spill -- PAHs in North-West Japan Sea -- PAHs in South-East China Sea -- Part 6.Metabolic Activation/Toxicities -- Metabolic Activation -- Carcinogenicity/Mutagenicity -- Endocrine Disruption -- ROS Production -- Toxicities in Fish and Other Marine Organisms -- Part 7.Environmental Standards/Guidelines -- Environmental Standards/Guide Lines of PAHs/NPAHs (including environmental standards/guide lines in Japan and in the world
    Abstract: This book reviews Polycyclic Aromatic Hydrocarbons (PAHs) and Nitropolycyclic Aromatic Hydrocarbons (NPAHs) contamination in the context of environmental pollution in Asia. It is comprised of the following sections: 1. Fundamental Chemistry and General Characteristics; 2. Analytical Methods; 3. Emission Source and Atmospheric Behavior; 4. Atmospheric Polycyclic Aromatic Hydrocarbons and PM2.5; 5. Polycyclic Aromatic Hydrocarbons in Marine Environments; 6. Metabolic Activation/Toxicities; and 7. Environmental Standards and Guidelines. This volume concentrates on the Far East due to the massive consumption of coal and petroleum in China, which has led to considerable levels of air pollution. High concentration of atmospheric PM2.5 in Beijing have been reported since January 2013 and exposure to such high concentrations may cause respiratory, cardiac and lung diseases. Gathering contributions from international experts, this volume provides a valuable reference guide for global researchers and students interested in learning from the East Asian experience
    Pages: XIII, 274 p. 99 illus., 40 illus. in color. : online resource.
    ISBN: 9789811067754
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  • 9
    Keywords: Medicine ; Gene Expression ; Medical genetics ; Cell Biology ; Biomedicine ; Gene Function ; Gene Expression ; Cell Biology ; Springer eBooks
    Abstract: The three-dimensional organization of the DNA inside the eukaryotic cell nucleus has emerged a critical regulator of genome integrity and function. Increasing evidence indicates that nuclear pore complexes (NPCs), the large protein channels that connect the nucleus to the cytoplasm, play a critical role in the establishment and maintenance of chromatin organization and in the regulation of gene activity. These findings, which oppose the traditional view of NPCs as channels with only one: the facilitation of nucleocytoplasmic molecule exchange, have completely transformed our understanding of these structures. This book describes our current knowledge of the role of NPCs in genome organization and gene expression regulation. It starts by providing an overview of the different compartments and structures of the nucleus and how they contribute to organizing the genome, then moves to examine the direct roles of NPCs and their components in gene expression regulation in different organisms, and ends by describing the function of nuclear pores in the infection and genome integration of HIV, in DNA repair and telomere maintenance, and in the regulation of chromosome segregation and mitosis. This book provides an intellectual backdrop for anyone interested in understanding how the gatekeepers of the nucleus contribute to safeguarding the integrity and function of the eukaryotic genome.℗
    Pages: VIII, 240 p. 32 illus. in color. : online resource.
    ISBN: 9783319716145
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  • 10
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia -- Detection of RB1 Gene Copy Number Variations using a Multiplex Ligation-Dependent Probe Amplification Method -- A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene -- Using℗ Methylation-specific PCR to study RB1 Promoter Hypermethylation -- Detection of Aberrant DNA Methylation Patterns in the RB1 Gene -- Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis -- Immunohistochemical Detection of the Retinoblastoma Protein -- Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples -- Detection of CCND1 Locus Amplification by Fluorescence in situ Hybridization -- Detection of CCND1 Gene Copy Number Variations using Multiplex Ligation-dependent Probe Amplification and Fluorescence in situ Hybridization Methods -- Detection of p16 Promoter Hypermethylation by Methylation-specific PCR -- Immunohistochemical Detection of p16 in Clinical Samples -- Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays -- Detection of E2F-induced Transcriptional Activity Using a Dual Luciferase Reporter Assay -- Detection of HPV E6/E7 mRNA in Clinical Samples using RNA in situ Hybridization -- CRISPR/Cas9 Mediated Knockout of Rb1 in Xenopus tropicalis
    Abstract: This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ The Retinoblastoma Protein℗ aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor
    Pages: XI, 200 p. 38 illus., 32 illus. in color. : online resource.
    ISBN: 9781493975655
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  • 11
    Keywords: Medicine ; Biomedicine ; Biomedicine general ; Springer eBooks
    Description / Table of Contents: ssociation Between Structure and Toxicity of Enterotoxigenic℗ Escherichia coli℗ (ETEC) -- Heat-Stable Toxin Ib (STa) -- Bacillus anthracis℗ Toxins: Efficient Biochemical Weapons for the Infectious Battle -- Bacterial Genotoxins -- Bacterial Pore-Forming Toxin in Macromolecule Delivery: A Lesson Learned from Listeriolysin O -- Chaperones and ADP-ribosylating Bacterial Toxins -- Clostridium perfringens℗ Epsilon Toxin -- Clostridium perfringens℗ Iota Toxin: A Successfully-Shared Template for Common Enteric Pathogens. ́€“ Colibactin. ́€“ Cyanotoxins -- Escherichia coli℗ Shiga Toxins -- Escherichia coli℗ STb Toxin -- Food Poisoning in Bangladesh -- Glycosphingolipids as Toxin Receptors.℗ Helicobacter pylori℗ CagA: The Bacterial Oncoprotein -- Interaction of℗ Helicobacter pylori℗ VacA Toxin with Its Target Cells -- Membrane Degrading Toxins -- Mode of Action of Cry Toxins fromBacillus thuringiensisi&a mp;g t;℗ and Resistance Mechanisms -- Mycotoxin: Review and Detection -- Overview of℗ Burkholderi pseudomallei℗ Toxins and Clinical Implications -- Role of Bacterial Toxins in Enterotoxemia of Monogastrics and Ruminants -- Role of℗ Clostridium difficileToxins in Antibiotic Associated Diarrhea and Pseudomembranous Colitis -- Role ofClostridium perfringens℗ Toxins in Necrotic Enteritis in Poultry -- Role of Listeria Monocytogenes Toxins in Virulence -- RTX Toxins: A Review -- Structure Function Studies of Large Clostridial Cytotoxins -- Tetanus and Botulinum Neurotoxins -- Toxins ofStaphylococcus aureus: An Arsenal of Complementary Weapons -- Translocation of Toxins by Gram-Negative Pathogens Using the Type III Secretion System. ℗
    Abstract: This volume provides an overview of microbial toxins from diverse bacterial and fungal origins. These molecules, produced by various species and consisting of protein or small organic molecules, can play a pivotal role in pathogenesis of plants, animals, and humans that in turn can lead to the survival/dissemination of the host microbe. Many of these microbes, due to their toxins, impact our society from a health and economic perspective. In particular, this volume address the diverse niches of these organisms focused upon their associated toxins. The structures, functions, and genetics of toxins will be addressed. Besides the ill-effects elicited by these toxins, it must be noted that there is immense potential for turning some of these harmful molecules into useful tools as specific probes (of receptors), novel drugs or drug-conjugates for the treatment of various diseases, and immunomodulating agents for targeted vaccine delivery for research and unique medicines. Recent progress in bacterial genome mapping and establishment of three-dimensional crystal structures of several bacterial toxins provides a deeper knowledge and greater understanding of structure-function relationships. Moreover, the emergence of some bacteria (i.e.,℗ Bacillus anthracis,℗ Burkholderia pseudomallei,℗ Staphylococcus aureus), and their toxins, as biological weapons also necessitates a thorough understanding of these agents, their pathophysiology, and development of countermeasures. This volume will also be a common resource for researchers interested in many other medically-relevant microorganisms, and their toxins, that include℗ Clostridium botulinum, C. difficile, C. perfringens, C. tetani, Escherichia coli, Helicobacter pylori,℗ and℗ Listeria monocytogenes.℗ Recent studies℗ have correlated℗ the effect of global warming and climate change as a trigger for natural disasters and impact on human health℗ via emergence of various vector-borne and infectious diseases caused by toxin-producing microbes. The 2003 Institute of Medicine (IOM) report, ́€œMicrobial Threats to Health,́€℗ identified the influence of changing ecosystem on the emergence and spread of infectious diseases and economic development of specific regions of the world.℗ This current handbook is a major reference work on microbial toxins written by a panel of International experts and organized into 24 chapters
    Pages: 77 illus., 59 illus. in color. eReference. : online resource.
    ISBN: 9789400764491
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  • 12
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma -- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays -- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens -- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens -- Isolation of GBM Stem Cells with Flow Cytometry -- Lentiviral Transduction of Primary Human Glioblastoma Cultures -- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors -- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF -- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro -- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry -- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS) -- Single-Cell RNA Sequencing of Glioblastoma Cells -- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures -- Orthotopic Patient-derived Glioblastoma Xenografts in Mice -- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice -- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy -- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection -- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma -- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages.℗
    Abstract: This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture microdissection, and modeling human glioma with human embryonic stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glioblastoma: Methods and Protocols aims to support researchers seeking new and refined protocols to better decrypt this tumor.℗
    Pages: XI, 241 p. 52 illus., 43 illus. in color. : online resource.
    ISBN: 9781493976591
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  • 13
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Extracellular RNAs ́€“ A New Awareness of Old Perspectives -- Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles -- Extracellular RNA Isolation from Cell Culture Supernatant -- Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture -- Isolation of Extracellular RNA from Serum/Plasma -- Isolation of Extracellular RNA from Bile -- Cushioned́€“Density Gradient Ultracentrifugation (Ć€“DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes -- Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids -- Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography -- Detection and Analysis of Non-vesicular Extracellular RNA -- Isolation of Plasma Lipoproteins as a Source of Extracellular RNA -- Droplet Digital PCR for Quantitation of Extracellular RNA -- Preparation of Small RNA NGS Libraries from Biofluids -- Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString -- Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs -- Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs.℗
    Abstract: This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field.℗
    Pages: XI, 216 p. 43 illus., 30 illus. in color. : online resource.
    ISBN: 9781493976522
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  • 14
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Detection of Combinatorial Mutational Patterns in Human Cancer Genomes by Exclusivity Analysis -- Discovering Altered Regulation and Signaling Through Network-based Integration of Transcriptomic, Epigenomic, and Proteomic Tumor Data -- Analyzing DNA Methylation Patterns during Tumor Evolution -- MicroRNA Networks in Breast Cancer Cells -- Identifying Genetic Dependencies in Cancer by Analyzing siRNA Screens in Tumor Cell Line Panels -- Phosphoproteomics-based Profiling of Kinase Activities in Cancer Cells -- Perseus: A Bioinformatics Platform for Integrative Analysis of Proteomics Data in Cancer Research -- Quantitative Analysis of Tyrosine Kinase Signaling Across Differentially Embedded Human Glioblastoma Tumors -- Prediction of Clinical Endpoints in Breast Cancer using NMR Metabolic Profiles -- Stochastic and Deterministic Models for the Metastatic Emission Process: Formalisms and Crosslinks -- Mechanically Coupled Reaction-Diffusion Model to Predict Glioma Growth: Methodological Details -- Profiling Tumor Infiltrating Immune Cells with CIBERSORT -- Systems Biology Approaches in Cancer Pathology -- Bioinformatics Approaches to Predict Drug Responses from Genomic Sequencing -- A Robust Optimization Approach to Cancer Treatment under Toxicity Uncertainty -- Modeling of Interactions between Cancer Stem Cells and their Microenvironment: Predicting Clinical Response -- Methods for High-throughput Drug Combination Screening and Synergy Scoring.℗
    Abstract: This book comprises protocols describing systems biology methodologies and computational tools,℗ offering a variety of ways to analyze different types of high-throughput cancer data.℗ Chapters give an overview over data types available in large-scale data repositories and state-of-the-art methods used in the field of cancer systems biology. ℗ Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ Cancer Systems Biology℗ : Methods and Protocols℗ aims to ensure successful results in the further study of this vital field. The chapters "Identifying Genetic Dependencies in Cancer by Analyzing siRNA Screens in Tumor Cell Line Panels", "Perseus: A Bioinformatics Platform for Integrative Analysis of Proteomics Data in Cancer Research" and "Phosphoproteomics-based Profiling of Kinase Activities in Cancer Cells" are℗ available open access under a CC BY 4.0 license via link.springer.com
    Pages: XI, 402 p. 86 illus., 76 illus. in color. : online resource.
    ISBN: 9781493974931
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  • 15
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Nutritional programming effects on development of metabolic disorders in later life -- Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease -- Developmental Origins of Stress and Psychiatric Disorders -- Proteomic Studies of Psychiatric Disorders -- Developmental Origins of Breast Cancer: A Paternal Perspective -- Point-of-care Testing and Personalized Medicine for Metabolic Disorders -- Pregnancy and Lactation a Window of Opportunity to Improve Individual Health -- Utility of Small Animal Models of Developmental Programming -- Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents -- Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders -- Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models -- Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction -- Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders -- Assessment of Placental Transport Function in Studies of Disease Programming -- Assessment of Fatty Liver in Models of Disease Programming -- Capillary Blood Sampling from the Finger -- Physical Activity Assessment in Clinical Studies of Substance use Disorder -- Cardiopulmonary Exercise Testing -- Cardiovascular Assessment in Human Research -- Nutritional Programming Effects on the Immune System -- Small RNA Sequencing - A Technique for miRNA Profiling -- Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function -- Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries -- Telomere length analysis - A Tool for Dissecting Ageing Mechanisms in Developmental Programming -- Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics -- In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models -- Experimental Model of Skeletal Muscle Laceration in Rats -- Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner’s Guide to Behavioural Analysis -- Hyperlocomotion Test for Assessing Behavioural Disorders -- 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation -- Mass Spectrometry Profiling of Pituitary Glands -- Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation -- Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum -- Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry -- Lab-on-a-chip Device for Rapid Measurement of Vitamin D Levels -- Kidney Smartphone Diagnostics -- A User-friendly App for Blood Coagulation Disorders -- Hormonal Smartphone Diagnostics
    Abstract: This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or therapeutic area. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications aim is to provide insights into the latest ideas and technologies .enabling progress in this field
    Pages: XIV, 520 p. 101 illus., 88 illus. in color. : online resource.
    ISBN: 9781493976140
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  • 16
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Detection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs -- ℗ Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays.℗
    Abstract: This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.℗ Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field.℗
    Pages: XIII, 220 p. 50 illus., 33 illus. in color. : online resource.
    ISBN: 9781493975624
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  • 17
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer -- Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer -- Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer -- Modelling Intestinal Carcinogenesis Using In Vitro OrganoidCultures -- Protocols to Evaluate Cigarette Smoke-induced Lung Inflammationand Pathology in Mice -- Tracking Competent Host Defence to Chronic Inflammation: An In vivo Model of Peritonitis -- Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology -- In Vivo Infection Model of Severe Influenza a Virus -- In Vivo Models for Inflammatory Arthritis -- Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease -- Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer -- Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models -- Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease -- Quantifying Caspase-1 Activity in Murine Macrophages -- Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation -- Analysis of DNA Methylation in Tissues Exposed to Inflammation -- A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens -- Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18 -- Optimisation Techniques for miRNA Expression in Low Frequency Immune Cell Populations -- Assessing the cGAS-cGAMP-STING Activity of Cancer Cells -- Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies -- Production of Recombinant Killer Immunoglobulin-like Receptors for Crystallography and Luminex Based Assays -- Isolation and Characterisation of Mouse Intrahepatic Lymphocytes by Flow Cytometry
    Abstract: This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. In addition, each chapter includes notes to provide tips avoiding known pitfalls, troubleshooting advice and elaborate further on specific materials or methods. ℗ Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field
    Pages: XII, 315 p. 16 illus. : online resource.
    ISBN: 9781493975686
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  • 18
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume discusses techniques to synthesize and functionalize nanoparticles, monitor their delivery and uptake, and identify and evaluate their lethal and non-lethal effects on the metabolic activity of the nervous system. The chapters in this book are divided into 4 sections: photo-stimulation, thermal stimulation, mechanical perturbation, and toxicity and physiological effects. The first 3 sections ℗ focus on nanoparticle interactions with external sources that disturb the neuronal system, while the 4th explores the effects of having nanoparticles in a neuronal system in the absence of external stimuli. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Use of Nanoparticles in Neuroscience is a valuable resource for graduate students and specialized researchers that want to learn techniques needed to quantify experiments that use nanoparticles in the nervous system
    Pages: XVI, 296 p. 98 illus., 66 illus. in color. : online resource.
    ISBN: 9781493975846
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  • 19
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Abstract: This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography, ℗ angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences
    Pages: XV, 352 p. 58 illus., 41 illus. in color. : online resource.
    ISBN: 9781493975952
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  • 20
    Keywords: Medicine ; Cancer Research ; Neurosciences ; Developmental Biology ; Evolutionary Biology ; Neurobiology ; Biomedicine ; Neurosciences ; Evolutionary Biology ; Cancer Research ; Developmental Biology ; Neurobiology ; Springer eBooks
    Abstract: This book provides a comprehensive overview of ZIC-family research. Part1 summarizes the ZIC family in animal evolution and development and presents an overall picture of the ZIC family gene structure in eumetazoan animals, providing an evolutionary hypothesis and reviewing the studies on the role of ZIC family proteins in developmental processes for each animal model. Part 2 shows that the ZIC family are the causative genes for developmental disorders, and discusses the role of the ZIC family in stem cell biology. It also presents studies on the ZIC family in the medical biology field. This interdisciplinary book is a valuable resource not only for those people directly involved in ZIC-family-related research, but also researchers in diverse research fields who are interested in the latest advances in biology and medicine
    Pages: X, 380 p. 105 illus., 69 illus. in color. : online resource.
    ISBN: 9789811073113
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  • 21
    Keywords: Medicine ; Pharmacology ; Epidemiology ; Environmental Health ; Biomedicine ; Pharmacology/Toxicology ; Epidemiology ; Environmental Health ; Springer eBooks
    Description / Table of Contents: Introduction -- Combined exposures.-Measuring combined exposures -- Modeling complex exposures.-Prioritizing mixtures for study -- Environment-Wide Association Studies -- Biogeographical analysis of chemical co-occurrence -- Combining exposure and toxicity information to develop logical groupings for assessment -- Maximum Cumulative Ratio for deciding when to assess mixtures -- Establishing the “no interaction” baseline -- Dose addition models -- Response addition -- Integrated addition -- Mixture statistics – comparing predicted to observed -- Cumulative Risk Assessment -- Deciding which approach to use -- Component-based approaches -- Sufficient similarity of whole mixtures -- Other whole mixtures approaches -- Assessing interactions -- Likelihood and examples of chemical interactions -- PBPK modeling of chemical interactions -- Adding interactions to cumulative risk assessment -- Consideration of nonchemical stressors -- Toxicity and interactions of physical and chemical stressors -- Psychosocial and chemical stressors -- Adding nonchemical stressors to cumulative risk assessments -- Disease-based risk assessment -- Community-based risk assessment
    Abstract: Exposure to multiple chemical and nonchemical stressors is the rule, not the exception. Nevertheless, it continues to be a significant challenge in toxicology and risk assessment to thoroughly take mixtures into account. In this book, both basic and advanced concepts for considering mixtures are discussed, from initial-exposure characterization through the evaluation of risk associated with combined exposures. Several chapters also examine the adoption of promising tools from other disciplines. Looking beyond chemical mixtures, the discussion also addresses incorporating nonchemical stressors into toxicity studies and cumulative-risk assessments. This €œéxcellent́€ volume€”ás described by Linda S. Birnbaum, Director of the National Institute of Environmental Health Sciences and the National Toxicology Program, in her foreword to the booḱ€”forms €œá comprehensive picture of the current state of mixtures science.́€ With contributions from a host of established experts, Chemical Mixtures and Combined Chemical and Nonchemical Stressors: Exposure, Toxicity, Analysis, and Risk is an essential text for mixtures researchers in the fields of toxicology, epidemiology, exposure science, risk assessment, and statistics. Both seasoned mixtures researchers and those new to the field will appreciate the clear explanations of mixtures concepts as well as the comprehensive presentation of available tools for predicting mixture effects and estimating risk from combined exposures. This volume also serves as a useful reference book for students.℗
    Pages: XIV, 556 p. 77 illus., 54 illus. in color. : online resource.
    ISBN: 9783319562346
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  • 22
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Abstract: 5-HT2A receptors are G-protein coupled receptors that are widely distributed throughout the brain, most notably on neuronal and glial cells. 5-HT2A receptors have been implicated in various central physiological functions including mood regulation, memory, sleep, nociception, eating, and reward behaviors, and they are also believed to control the cardiovascular system. This℗ book provides a comprehensive overview of these receptors including sections on their properties and distribution, approaches for their study, their role in a number of brain functions and diseases, and their role as therapeutic targets
    Pages: XII, 446 p. 69 illus., 23 illus. in color. : online resource.
    ISBN: 9783319704746
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  • 23
    Keywords: Medicine ; Human Genetics ; Molecular Biology ; Pharmacology ; Psychiatry ; Biochemistry ; Biomedicine ; Human Genetics ; Psychiatry ; Pharmacology/Toxicology ; Molecular Medicine ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Prevalence of Smoking and Its Associated Diseases.-℗ Addiction Genetics: Basic Concepts and Techniques.-℗ Estimation of Genetic and Environmental Contributions to Smoking Addiction -- Identified Susceptibility Loci for Nicotine Addiction Based on Genome-Wide Linkage Analyses -- Involvement of Variants in Gene Clusters℗ CHRNA5/A3/B4℗ on Chromosome 15 to Smoking Behaviors and Lung Cancer.-℗ Contribution of Variants in℗ CHRNB3/A6℗ Gene Cluster on Chromosome 8 to Smoking Dependence.-℗ Genetic Contribution of Variants in GABAergic Signaling to Nicotine Dependence.-℗ Contribution of Variants in℗ DRD2/ANKK1℗ on Chromosome 11 with Smoking and Other Addictions.-℗ Significant Contribution of Variants in Serotonin Transporter and Receptor Genes to Smoking Dependence.-℗ Converging Findings from Linkage and Association Analyses on Susceptibility Genes for Smoking Addiction.-℗ Contribution of Gen逓Gene and Gene€“Énvironmental Interactions to Tobacco Smoking.-℗ Identification of Biological Pathways Associated with Smoking Initiation/Progression, Nicotine Dependence, and Smoking Cessation.-℗ Neuroproteomics and Its Applications in Research on Nicotine and Other Drugs of Abuse.-℗ Regulatory Roles of MicroRNAs in Addictions and Other Psychiatric Diseases.-℗ Tobacco Smoking, Food Intake, and Weight Control.-℗ Nicotine Modulates Innate Immune Pathways via℗ Îł7 Nicotinic Acetylcholine Receptor.-℗ DNA Methylation Analysis Reveals a Strong Connection between Tobacco Smoking and Cancer Pathogenesis.-℗ Evolutionary Relations℗ of Genes Encoding Nicotinic Acetylcholine Receptor Subunits.-℗ Management, Pharmacotherapies, and Precision Medicine for Smoking Cessation.-℗ Background and Biology of and Health Concerns about Electronic Cigarettes.-℗ Grand Challenges and Opportunities for Psychiatry, Including Nicotine Addiction Research
    Abstract: This book provides the most recent knowledge on almost all key aspects of the health impact of tobacco smoking.℗ ℗ Its 21 chapters focus on both preclinical and clinical studies. The contents are broad, covering the epidemiology of tobacco smoking; genetic epidemiology; identification of susceptibility genomic regions, genes, and pathways as determined by both human and animal studies; evolutionary relations among the different nAChR subunit genes that are so important to the nicotine response; smoking-related diseases; E-cigarettes; and smoking cessation. Furthermore, each chapter includes a detailed and comprehensive list of key references.℗ ℗ For both clinical and basic researchers, this book is a valuable resource on nicotine dependence and other addictions
    Pages: XIII, 365 p. 44 illus., 33 illus. in color. : online resource.
    ISBN: 9789811075308
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  • 24
    Keywords: Medicine ; Biomedical Engineering ; Biotechnology ; Systems Biology ; Biomaterials ; Biomedicine ; Biomedical Engineering/Biotechnology ; Biomedical Engineering ; Systems Biology ; Biomaterials ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Introduction -- The Minimal Cell -- Synthetic Proteins -- Biomimicry - Bottom-Up Approach -- The Tools -- Dealing with the Dangers
    Abstract: This textbook discusses the new relationship between artificial, synthetic material and living matter, and presents defined examples of approaches aiming for the creation of artificial cells. It also offers insights into the world of synthetic biology from its origins to the present day, showing what is currently possible in this discipline. Furthermore, it examines the ethical concerns and potential threats posed by this new field. The textbook is based on a lecture of the same title, held for master’s students at the University of Natural Resources and Life Sciences (BOKU), Vienna, and is primarily intended for students of synthetic biology, biotechnology and bioengineering. It is also of interest to research scientists from other disciplines wishing to learn more about the state of the art of synthetic biology and its future
    Pages: VI, 65 p. 51 illus. in color. : online resource.
    ISBN: 9783319731230
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  • 25
    Keywords: Medicine ; Neurosciences ; Otorhinolaryngology ; Neurobiology ; Biomedicine ; Neurosciences ; Otorhinolaryngology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Mammalian Auditory Pathways -- Overview of Auditory Projection Pathways and Intrinsic Microcircuits.-℗ Microcircuits of the Ventral Cochlear Nucleus.-℗ Microcircuits of the Dorsal Cochlear Nucleus.-℗ Integration of Synaptic and Intrinsic Conductances Shapes Microcircuits in the Superior Olivary Complex.-℗ Neurons, Connections, and Microcircuits of the Inferior Colliculus.-℗ Sensing Sound Through Thalamocortical Afferent Architecture and Cortical Microcircuits.-℗ Auditory Cortex Circuits.-℗ Circuits for Modulation of Auditory Function -- Index
    Abstract: This volume presents an overview of the functional anatomy of the central auditory system integrated with cell biology, synaptic physiology, and electrophysiology. ́€Ø Introduction to Mammalian Auditory Pathways Douglas L. Oliver and Nell B. Cant ́€Ø Overview of Auditory Projection Pathways and Intrinsic Microcircuits℗ Nell B. Cant and Douglas L. Oliver ́€Ø Microcircuits of the Ventral Cochlear Nucleus Maria E. Rubio ́€Ø Microcircuits of the Dorsal Cochlear Nucleus Laurence O. Trussell and Donata Oertel ́€Ø Integration of Synaptic and Intrinsic Conductances Shapes℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ Microcircuits in the Superior Olivary Complex Conny Kopp-Scheinpflug and Ian D. Forsythe ́€Ø Neurons, Connections, and Microcircuits of the Inferior Colliculus Tetsufumi Ito and Manuel S. Malmierca ́€Ø Sensing Sound Through Thalamocortical Afferent Architecture and℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ Cortical Microcircuits Heather L. Read and Alex D. Reyes ́€Ø Auditory Cortex Circuits Eike Budinger and Patrick Kanold ́€Ø Circuits for Modulation of Auditory Function ℗ ℗ ℗ ℗ Brett R. Schofield and Laura Hurley Douglas S. Oliver is Professor in the Department of Neuroscience, UConn Health, University of Connecticut, Farmington Nell B. Cant is Professor in the Department of Neurobiology, Duke University Medical Center, Durham Richard R. Fay is Distinguished Research Professor of Psychology at Loyola University Chicago Arthur N. Popper is Professor Emeritus and research Professor in the Department of Biology at the University of Maryland, College Park
    Pages: XVIII, 267 p. : online resource.
    ISBN: 9783319717982
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  • 26
    Keywords: Medicine ; Cancer Research ; Molecular Biology ; Biomedicine ; Cancer Research ; Molecular Medicine ; Springer eBooks
    Abstract: Cancer is a multifaceted disease and overwhelmingly increasing experimental evidence has helped us to develop a deeper understanding of the role of signal transduction cascades in cancer development and progression. Tissue microarrays and next generation sequencing technologies have assisted us to gather missing pieces of jigsaw puzzle and we now know that deregulation of spatio-temporally controlled signaling cascades play fundamental role in metastasis and resistance against wide ranging therapeutics. This book offers a balanced overview of the rapidly emerging cutting edge research in molecular oncology and good source of knowledge for established oncologists, basic and medical students and pharmaceutical industry associated R&D departments
    Pages: XII, 308 p. 16 illus. in color. : online resource.
    ISBN: 9783319715537
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  • 27
    Keywords: Medicine ; Medical genetics ; Neurosciences ; Neurology ; Biomedicine ; Neurosciences ; Neurology ; Gene Function ; Springer eBooks
    Abstract: This book provides a cutting-edge review of polyglutamine disorders. It primarily focuses on two main aspects: (1) the mechanisms underlying the pathologieś€™ development and progression, and (2) the therapeutic strategies that are currently being explored to stop or delay disease progression.℗ Polyglutamine (polyQ) disorders are a group of inherited neurodegenerative diseases with a fatal outcome that are caused by an abnormal expansion of a coding trinucleotide repeat (CAG), which is then translated in an abnormal protein with an elongated glutamine tract (Q). To date, nine polyQ disorders have been identified and described: dentatorubral-pallidoluysian atrophy (DRPLA); Huntingtoń€™s disease (HD); spina倓bulbar muscular atrophy (SBMA); and six spinocerebellar ataxias (SCA 1, 2, 3, 6, 7, and 17). The genetic basis of polyQ disorders is well established and described, and despite important advances that have opened up the possibility of generating genetic models of the disease, the mechanisms that cause neuronal degeneration are still largely unknown and there is currently no treatment available for these disorders. Further, it is believed that the different polyQ may share some mechanisms and pathways contributing to neurodegeneration and disease progression
    Pages: VIII, 469 p. 41 illus., 39 illus. in color. : online resource.
    ISBN: 9783319717791
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  • 28
    Keywords: Medicine ; Biomedical Engineering ; Microbiology ; Microbial genetics ; Microbial genomics ; Biomedicine ; Biomedical Engineering/Biotechnology ; Microbial Genetics and Genomics ; Applied Microbiology ; Springer eBooks
    Description / Table of Contents: Section 1: Application of microbial technology in food -- 1.Recent food preservation techniques employed in the food industry -- 2.Metagenomic insights into environmental microbiome and their application in food/pharmaceutical industry -- 3.Changing paradigm of probiotics from functional foods to biotherapeutic agents -- 4.Microbial Valorization of Chitinous Bioresources for Chitin Extraction and Production of Chito-Oligomers and N-acetylglucosamine: Trends, Perspectives and Prospects -- 5.Seaweed Liquid Fertilizers: A Novel Strategy for the Biofortification of VegeTables and Crops -- 6.Green algae biomass cultivation, harvesting and genetic modifications for enhanced cellular lipids -- 7.Probiotics: The ultimate nutritional supplement -- 8.An insight into the prevalence and enzymatic abatement of urethane in fermented beverages -- 9.Sea water as a reaction medium for bioethanol production -- 10.Bacterial mediated plant protection: Induced systemic resistance in soybean -- 11.Mycotoxins and Pesticides: Toxicity and Applications in Food and Feed -- 12.Microbes and their role in drought tolerance of agricultural food crops -- 13.Microbial Remediation of Persistent Agro-Chemicals by Soil Bacteria: An Overview -- Section 2: Application of microbial technology in pharmacology -- 14.Insectivorous plants of India: Sources of bioactive compounds to fight against antimicrobial resistance -- 15.Exploring the multifaceted role of microbes in pharmacology -- 16.Pharmacological Applications of Metabolites of Mangrove Endophytes: A Review -- 17.Application of oncolytic virus as a therapy of cancer -- 18.Microbes in the treatment of Diabetes and its complications -- 19.Microbe-based metallic nanoparticles synthesis and biomedical applications: An update -- 20.Role of Silver Nanoparticles in Treatment of Plant Diseases -- 21.Endophytic fungi and bioactive metabolites production: An update -- 22.Fungal endophytes from seaweeds: an overview -- 23.Probiotic biosurfactants: A potential therapeutic exercises in biomedical sciences -- 24.Recent antibiotics used in dental disease management
    Abstract: This edited book, is a collection of 25 chapters describing the recent advancements in the application of microbial technology in the food and pharmacology sector. The main focus of this book is application of microbes, food preservation techniques utilizing microbes, probiotics, seaweeds, algae, enzymatic abatement of urethane in fermentation of beverages, bioethanol production, pesticides, probiotic biosurfactants, drought tolerance, synthesis of application of oncolytic viruses in cancer treatment, microbe based metallic nanoparticles, agro chemicals, endophytes, metabolites, antibiotics etc. This book highlighted the significant aspects of the vast subject area of microbial biotechnology and their potential applications in food and pharmacology with various topics from eminent experts around the World. This book would serve as an excellent reference book for researchers and students in the Food Science, Food Biotechnology, Microbiology and Pharmaceutical fields
    Pages: XVIII, 537 p. 122 illus., 33 illus. in color. : online resource.
    ISBN: 9789811071409
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  • 29
    Keywords: Medicine ; Human Physiology ; Rehabilitation ; Sports Medicine ; Biomedicine ; Human Physiology ; Sports Medicine ; Rehabilitation ; Springer eBooks
    Description / Table of Contents: Introduction -- Cycling -- Force, Velocity and Power in Cycling -- Pedalling Technique, Effectiveness and Coordination -- Influence of Gear Ratios -- Measurements on Cycle Ergometers -- Ballistic Movements of Lower and Upper Limbs: Jumping and Pushing.-Simple Method for Measuring Jump Height and Power during Rebound and Drop Jump -- Simple Method for Measuring Force, Velocity and Power during Jumping -- Optimal Force-Velocity Profile in Jumping: Measurement and Relationship with Performance -- Simple Method for Measuring Stiffness during Hopping -- Simple Method for Measuring Force, Velocity, Power and Force-Velocity profile of Upper Limbs -- Running -- Simple Method for Measuring Lower Limb Stiffness during Running -- Measuring the Energetic Cost of Sprint Running -- Simple Method for Measuring Force, Velocity, Power and Force-Velocity profile and Optimal Force-Velocity profile during Sprint Running -- Measurements on Sprint Instrumented Treadmills -- Effectiveness of Force Production during Sprint Running
    Abstract: This book presents an account of innovative methods and, for most of them, gives direct and practical insights into how practitioners can benefit from their use in their everyday practice. It also explains how to interpret the data measured, and the underlying neuromechanical and biomechanical factors related to sports performance. Written and edited by the same researchers who proposed and validated these methods, this book not only presents innovative methods for an efficient training and testing process (most of which are based on very simple technology and data processing methods), but also discusses the associated background information. Although it is a young scientific discipline, sport biomechanics has taken on an important role in routine sports training, medicine and rehabilitation. It allows both a better understanding of human locomotion and performance and better design of training and injury prevention. In those processes, the testing of athletes is crucial, and the quality and quantity of the variables analysed directly influences the efficiency of physicians’, coaches’, physiotherapists’ and other practitioners’ interventions
    Pages: VIII, 314 p. 110 illus. : online resource.
    ISBN: 9783319056333
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  • 30
    Keywords: Medicine ; Proteins ; Biology / Technique ; Biomedicine ; Biomedicine general ; Protein Science ; Protein-Ligand Interactions ; Biological Techniques ; Springer eBooks
    Description / Table of Contents: Supramolecular Protein Ligands – Unexplored Teritory Of Potential Pharmacological Activity -- Supramolecular Congo Red As Specific Ligand Of Antibodies Engaged In Immune Complex -- Protein Conditioning For Binding Congo Red And Other Supramolecular Ligands -- Metal Ions Introduced To Proteins By Supramolecular Ligands -- Possible Mechanism Of Amyloidogenesis Of V Domains -- Supramolecular Structures As Carrier Systems Enabling The Use Of Metal Ions In Antibacterial Therapy -- Congo Red Interactions With Single-Walled Carbon Nanotubes
    Abstract: This book is an open access under a CC BY license. The subject of this book relates to protein ligands with particular structural and complexation properties. They are composed of self-assembled molecules, capable of penetrating as a unit into proteins outside the binding site. The ribbon-like supramolecular system only permits the penetration of self-assembled molecules into the protein-body and formation of stable complexes. Supramolecular Congo red and similar compounds fit these requirements. Destabilized protein fragments enable the penetration of such ligands, with susceptibility to supramolecular ligand binding often associated with protein function. As a result, complexation modifies their functional effects. The activity of enzymes is inhibited by arresting them in the complexed state, but “naturally irreversible” complexation as in the case of immune complexation, is enhanced instead. This property offers many attractive possibilities of using supramolecular ligands as described in this book
    Pages: XII, 136 p. 98 illus., 44 illus. in color. : online resource.
    ISBN: 9783319656397
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  • 31
    Unknown
    Cham : Springer International Publishing
    Keywords: Medicine ; Medical Microbiology ; Molecular Biology ; Biochemistry ; Biomedicine ; Molecular Medicine ; Biochemistry, general ; Medical Microbiology ; Springer eBooks
    Description / Table of Contents: Part I: A guide of metabolism during host-pathogen interactions -- 1. Cellular metabolism at a glance -- 2. Interaction between nutrition and metabolism -- 3. Interplay between metabolic sensors and cell signaling -- 4.℗ Alterations on cellular redox states upon infection and implications for host cell homeostasis -- 5. Mitochondrial bioenergetics and dynamics during infection -- 6. Computational systems biology of metabolism in infection -- 7. Metabolomic-based methods in diagnosis and monitoring infection progression -- Part II: Microbial metabolic adaptation and pathogen rewiring of host cell metabolism during infection -- 8. Metabolic host response to intracellular infections -- 9. Protozoan parasites auxotrophies and metabolic dependencies -- 10. Viral manipulation of the host metabolic network -- 11.℗ Metabolic regulation of innate immunity to fungal infection -- 12. Metabolic crosstalk between host and parasitic pathogens -- 13. Microbiome and gut dysbiosis
    Abstract: This book focuses on host–pathogen interactions at the metabolic level. It explores the metabolic requirements of the infectious agents, the microbial metabolic pathways that are dedicated to circumvent host immune mechanisms as well as the molecular mechanisms by which pathogens hijack host cell metabolism for their own benefit. Finally, it provides insights on the possible clinical and immunotherapeutic applications, as well as on the available experimental and analytical methods. The contributions break new ground in understanding the metabolic crosstalk between host and pathogen
    Pages: VIII, 476 p. 45 illus., 43 illus. in color. : online resource.
    ISBN: 9783319749327
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  • 32
    Unknown
    Cham : Springer International Publishing
    Keywords: Medicine ; Cancer Research ; Immunology ; Infectious Diseases ; Proteins ; Biomedicine ; Immunology ; Protein Science ; Infectious Diseases ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Introduction and history.-℗ Part I: Structure of the HSPs in relation to chaperoning peptides and proteins -- Chapter 1:℗ Hsp70-subsrate interactions -- Chapter 2:℗ Molecular Chaperone Inhibitors.-℗ Part II: Exposure of HSPs to immune cells -- Chapter 3:℗ Extracellular Heat Shock Proteins as Stress Communication Signals.-℗ Part III: Regulation of immune responses by extracellular HSPs -- Chapter 4:℗ The Heat Shock Protein-CD91 pathway and Tumor Immuno-surveillance -- Chapter 5:℗ Bridging the gaps in the vaccine development: Avant-garde vaccine approach with secreted heat shock protein gp96-Ig -- Chapter 6:℗ Regulation of the Extracellular Matrix by Heat Shock Proteins and Molecular Chaperones -- Chapter 7:℗ Heat shock protein mediated T cell responses in pathogen infections -- Chapter 8:℗ An ancestral immune surveillance system in the amphibian Xenopus connecting certain heat shock proteins with classical and nonclassical MHC class I molecules -- Chapter 9:℗ Inhibition of HSPs for Enhanced Immunity. ℗ ℗ ℗
    Abstract: Experts from around the world review the current field of the immunobiology of heat shock proteins, and provide a comprehensive account of how these molecules are spearheading efforts in the understanding of various pathways of the immune system. This one-stop resource contains numerous images to both help illustrate the research on heat shock proteins, and better clarify the field for the non-expert. Heat shock proteins (HSPs) were discovered in 1962 and were quickly recognized for their role in protecting cells from stress. Twenty years later, the immunogenicity of a select few HSPs was described, and for the past 30 years, these findings have been applied to numerous branches of immunology, including tumor immunology and immunosurveillance, immunotherapy, etiology of autoimmunity, immunotherapy of infectious diseases, and expression of innate receptors. While HSPs can be used to manipulate immune responses by exogenous administration, they appear to be involved in initiation of de novo immune responses to cancer and likely in the maintenance of immune homeostasis. ℗
    Pages: X, 185 p. 22 illus., 20 illus. in color. : online resource.
    ISBN: 9783319690421
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  • 33
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: An Introduction to Epitope Mapping -- Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography -- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations -- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors -- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers -- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing -- Epitope Mapping Using Yeast Display and Next Generation Sequencing -- B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay -- Array-In-Well Epitope Mapping of Phage-Displayed Antibodies -- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries -- Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains -- Kinetic Analysis and Epitope Binning Using Surface Plasmon Resonance -- Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry -- Antibody Specificity Profiling Using Protein Microarrays -- High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target Binding -- Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope Mapping -- Antibody Biomarker Discovery Via In Vitro Evolution of Peptides -- Bioinformatics Tools for the Prediction of T Cell Epitopes
    Abstract: This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field
    Pages: XI, 284 p. 49 illus., 38 illus. in color. : online resource.
    Edition: 3rd ed. 2018.
    ISBN: 9781493978410
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  • 34
    Keywords: Medicine ; Cancer Research ; Medical genetics ; Oncology ; Biomedicine ; Gene Function ; Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Part1 : GENETIC CAUSES AND ASSOCIATED PHENOTYPES- Lynch syndrome -- The molecular basis of Lynch-like syndrome -- Constitutional mismatch repair deficiency -- Mismatch repair proficient hereditary non-polyposis colorectal cancer -- Genetic and environmental modifiers of risk in Lynch syndrome -- ADENOMATOUS POLYPOSIS SYNDROMES- Introduction -- Familial adenomatous polyposis -- Polymerase proofreading-associated polyposis -- MUTYH-associated polyposis -- NTHL1-associated polyposis -- Germline biallelic inactivation of MMR genes (with polyposis phenotype) -- Unexplained adenomatous polyposis -- HAMARTOMATOUS POLYPOSIS SYNDROMES- Peutz-Jeghers syndrome -- Juvenile polyposis syndrome -- PTEN-hamartoma tumor syndromes -- Other hamartomatous polyposis conditions -- HEREDITARY MIXED POLYPOSIS SYNDROME -- SERRATED POLYPOSIS SYNDROME -- Part2: GENETIC DIAGNOSTICS and CLINICAL MANAGEMENT -- Genetic testing in hereditary colorectal cancer -- Universal tumor screening for Lynch syndrome -- Classification of genetic variants. Prediction models for Lynch syndrome -- Surveillance guidelines for hereditary colorectal cancer syndromes -- Surgical management of hereditary colorectal cancer syndromes -- Chemoprevention in hereditary colorectal cancer syndromes -- Immunotherapy in hereditary colorectal cancer -- The Immune Biology of Microsatellite Unstable cancer -- Hereditary colorectal cancer: Immunotherapy approaches -- Medical oncology management of hereditary colorectal cancer. Part3: REGISTRIES and DATABASES -- Databases: intentions, capabilities and limitations -- The Colon Cancer Family Registry Cohort -- The Prospective Lynch Syndrome Database -- The InSiGHT Database: An example LOVD system -- The International Mismatch Repair Consortium
    Abstract: This book provides information on a wide variety of issues ranging from genetics to clinical description of the syndromes, genetic testing and counseling, and clinical management including surveillance, surgical and prophylactic interventions, and chemoprevention. Moreover, current hot issues, such as the identification of novel causal genes and the challenges we face, and the relevance of cancer risk modifiers, both genetic and environmental, are also discussed. This reference book is great for geneticists, oncologists, genetic counselors, researchers, clinicians, surgeons and nurses dedicated to, or interested in, hereditary cancer. The best and most recognized experts in the field have contributed to this project, guaranteeing updated information, accuracy and the discussion of topical issues
    Pages: X, 505 p. 53 illus., 39 illus. in color. : online resource.
    ISBN: 9783319742595
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  • 35
    Unknown
    Cham : Springer International Publishing
    Keywords: Medicine ; Human Genetics ; Pharmaceutical technology ; Biomedicine ; Pharmaceutical Sciences/Technology ; Human Genetics ; Springer eBooks
    Abstract: The book Regulation of Heat Shock Protein Responses provides the most comprehensive review on contemporary knowledge on the regulation of HSP responses and its consequences to human diseases and disorders. Using an integrative approach to understanding the regulation of HSP responses, the contributors provide a synopsis of novel mechanisms by which HSP responses are regulated under normal physiological and pathophysiological conditions. Key basic and clinical research laboratories from major universities and academic medical hospitals around the world contribute chapters that review present research activity and importantly project the field into the future. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Human Physiology, Biotechnology, Molecular Medicine, Infectious Diseases and Pathology
    Pages: IX, 472 p. 99 illus., 79 illus. in color. : online resource.
    ISBN: 9783319747156
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  • 36
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Clinical Use and Efficacy of Ibritumomab in B-cell Lymphoma -- Biology and Pathology of B-cell lymphoma -- Resistance to Y-90 Ibritumomab Tiuxetan Therapy -- Features of Ibritumomab as Radionuclide Therapy -- Radiological Evaluation of Response and Resistance of Ibritumomab -- Characteristics of Ibritumomab as Radionuclide Therapy Agent -- Resistance and Heterogeneity of Intratumoral Antibody Distribution -- Radiation Dosimetry in Ibritumomab Therapy -- Combining RAIT and immune-based therapies to overcome resistance in cancer? -- Prospects for enhancing efficacy of radioimmunotherapy -- Index
    Abstract: This volume, in discussing resistance to ibritumomab, will focus on the mechanism, hematological aspects, radiological and nuclear medicine aspects, and medical physics that deal with radiation dosimetry, and will outline future prospects for overcoming resistance and enhancing efficacy of ibritumomab
    Pages: XIII, 158 p. 18 illus., 14 illus. in color. : online resource.
    ISBN: 9783319782386
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  • 37
    Keywords: Medicine ; Neurosciences ; Medicinal Chemistry ; Psychopharmacology ; Biomedicine ; Neurosciences ; Psychopharmacology ; Medicinal Chemistry ; Springer eBooks
    Description / Table of Contents: Preface -- Chemistry and structure-activity relationships of psychedelics -- Hallucinogens and serotonin 5-HT2A receptor-mediated signaling pathways -- Effects of hallucinogens on neuronal activity -- Interactions of Hallucinogens with the Glutamatergic System:℗ Permissive Network Effects Mediated Through Cortical Layer V Pyramidal Neurons -- The effects of hallucinogens on gene expression -- Effect of Hallucinogens on Unconditioned Behavior -- Hallucinogens in Drug Discrimination -- Phenomenology, structure and dynamic of psychedelic states -- Serotonergic hallucinogen-induced visual perceptual alterations -- New world tryptamine hallucinogens and the neuroscience of ayahuasca -- Experimental psychosis research and schizophrenia - similarities and dissimilarities in psychopathology -- A review of Hallucinogen Persisting Perception Disorder (HPPD) and an exploratory study of subjects claiming symptoms of HPPD -- Therapeutic applications of classic hallucinogens -- Classic Hallucinogens and Mystical Experiences: Phenomenology and Neural Correlates
    Abstract: This volume brings together the latest basic and clinical research examining the effects and underlying mechanisms of psychedelic drugs. Examples of drugs within this group include LSD, psilocybin, and mescaline. Despite their structural differences, these compounds produce remarkably similar experiences in humans and share a common mechanism of action.℗ Commonalities among the substances in this family are addressed both at the clinical and phenomenological level and at the basic neurobiological mechanism level. To the extent possible, contributions relate the clinical and preclinical findings to one another across species. The volume addresses both the risks associated with the use of these drugs and the potential medical benefits that might be associated with these and related compounds
    Pages: X, 430 p. 196 illus., 158 illus. in color. : online resource.
    ISBN: 9783662558805
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  • 38
    Keywords: Medicine ; Cancer Research ; Molecular Biology ; Pharmacology ; Infectious Diseases ; Biomedicine ; Pharmacology/Toxicology ; Cancer Research ; Molecular Medicine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Module 1. Introduction -- Module 2. Anti-diabetic action of thymoquinone -- Module 3. Anti-cancer action of thymoquinone -- Module 4. Anti-microbial action of thymoquinone -- Module 5. Hepatoprotective action of thymoquinone -- Module 6. Anti-epileptic action of thymoquinone -- Module 7. Anti-epileptic action of thymoquinone -- Module 8. Index
    Abstract: Throughout history black seeds, Nigella sativa seeds, have been highly revered for its medicinal properties. Thymoquinone (TQ), an active principle component of the volatile oil of black cumin seeds, is an emerging natural compound with a wide range of medical applications, and has several beneficial pharmacological actions i.e anti-oxidant, anti-diabetic, anti-inflammatory, anti-microbial, anti-tumor, anti-mutagenic, anti-epileptic, hepatoprotective, neuroprotective, and nephroprotective. As such, it is important to move TQ from the bench to bedside. This book illustrates the therapeutic importance of TQ, offering a detailed account of some of its molecular and therapeutic properties, and discussing in depth its anti-diabetic, anti-cancer, anti-oxidant, anti-inflammatory, anti-microbial, anti-epileptic and hepatoprotective actions. Lastly, the book examines the future prospects of TQ research and its use as a pharmaceutical
    Pages: XI, 85 p. 13 illus., 7 illus. in color. : online resource.
    ISBN: 9789811088001
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  • 39
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Resistance of colorectal tumors to anti-EGFR antibodies -- Resistance of lung cancer to kinase inhibitors specific to EGFR or ALK -- Mechanisms of action and resistance of trastuzumab in breast cancer -- Mechanisms of resistance to molecular therapies targeting the HGF/MET axis -- RAF, MEK and ERK inhibitors as anti-cancer drugs: intrinsic and acquired resistance as a major therapeutic challenge -- Mechanisms of resistance to PI3K and AKT inhibitors -- Sensitivity and resistance to BH3 mimetics in cancer therapy -- Resistance mechanisms to cyclin-dependent kinase inhibitors -- Resistance to inhibitors of angiogenesis
    Abstract: This volume comprehensively covers the multiplicity and diversity of mechanisms underlying patient resistance to currently approved anti-cancer drugs, including tyrosine kinase inhibitors and monoclonal antibodies, blockers of growth factor receptors and their downstream pathways, which play essential functions in cancer progression. Each chapter will cover a specific group of targets and the cognate drugs, along with molecular modes of innate and evolving resistance
    Pages: XX, 242 p. 22 illus. in color. : online resource.
    ISBN: 9783319679327
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  • 40
    Keywords: Medicine ; Immunology ; Laboratory Medicine ; Biomedicine ; Laboratory Medicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Foreword -- Preface -- Acknowledgements -- Introduction to the Immune System -- Antibody and antigen interaction -- Precipitation and Agglutination Reactions -- Rapid Plasma Reagin (RPR) Test -- Treponema pallidum heamagglutination (TPHA) Test -- Stained Brucella suspensions -- Rheumatoid Factor (RF) -- Suspension Anti-streptolysin-O (ASO/ASL) Test -- C-Reactive Protein (CRP) Latex Agglutination Test -- Complement fixation Test (CFT) -- Radioimmunoassay (RIA) -- Enzyme-immunoassay (EIAs) and Enzyme-linked immunosorbent (ELISA) -- Pregnancy Test -- Radial immunodiffusion (RID) -- Immunofixation electrophoresis (IFE) -- Immunofluorescence assay (IF)
    Abstract: This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing
    Pages: XV, 139 p. 51 illus. in color. : online resource.
    ISBN: 9783319776941
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  • 41
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Identifying Sequenced Eukaryotic Genomes and Transcriptomes with diArk -- An Introduction to the Saccharomyces Genome Database (SGD) -- Using the Candida Genome Database -- PomBase: The Scientific Resource for Fission Yeast -- EuPathDB: The Eukaryotic Pathogen Genomics Database Resource -- The Ensembl Genome Browser: Strategies for Accessing Eukaryotic Genome Data -- Mouse Genome Informatics (MGI) is the International Resource for Information on the Laboratory Mouse -- A Primer for the Rat Genome Database (RGD) -- Bovine Genome Database: Tools for Mining the Bos taurus Genome -- Navigating Xenbase: An Integrated Xenopus Genomics and Gene Expression Database -- Using ZFIN: Data Types, Organization and Retrieval -- EchinoBase: Tools for Echinoderm Genome Analyses -- A Multi-Omics Database for Parasitic Nematodes and Trematodes -- Using WormBase: A Genome Biology Resource for Caenorhabditis elegans and Related Nematodes -- Using WormBase ParaSite: An Integrated Platform for Exploring Helminth Genomic Data -- Using FlyBase to Find Functionally Related Drosophila Genes -- Hymenoptera Genome Database: Using HymenopteraMine to Enhance Genomic Studies of Hymenopteran Insects -- Navigating the i5k Workspace@NAL: A Resource for Arthropod Genomes
    Abstract: This volume explores databases containing genome-based data and genome-wide analyses. This book covers databases from all eukaryotic taxa, except plants. The chapters describe database contents and classic use-cases, which assist in accessing eukaryotic genomic data and encouraging comparative genomic research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Eukaryotic Genomic Databases: Methods and Protocols is a valuable resource for geneticists and molecular biologists who are interested in the latest eukaryotic genomics data. The chapters 'PomBase: The Scientific Resource for Fission Yeast' and 'The Ensembl Genome Browser: Strategies for Accessing Eukaryotic Genome Data' are available open access under a CC BY 4.0 license via link.springer.com
    Pages: XVI, 581 p. 259 illus., 257 illus. in color. : online resource.
    ISBN: 9781493977376
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  • 42
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Generation of Prostate Cancer Patient-derived Xenografts to Investigate Mechanisms of Novel Treatments and Treatment Resistance -- Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer -- Methodologies Applied to Establish Cell Cultures in Prostate Cancer -- Protocols for Migration and Invasion Studies in Prostate Cancer -- Transplantable Animal Studies and Whole-body Optical Imaging in Prostate Carcinoma -- Protocols for Tissue Microarrays in Prostate Cancer Studies -- Functional Studies on Steroid Receptors -- Protocols for Studies on TMPRSS/ERG in Prostate Cancer -- Protocols for the Study of Taxanes Chemosensitivity in Prostate Cancer -- A Method for Prostate and Breast Cancer Cell Spheroid Cultures using Gelatin Methacryloyl-based Hydrogels -- Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer -- Protocols for Studies on Stromal Cells in Prostate Cancer -- Techniques for Evaluation of AR Transcriptional Output and Recruitment to DNA -- NMR-based Prostate Cancer Metabolomics -- Studies on Steroid Receptor Coactivators in Prostate Cancer
    Abstract: This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function. Chapters detail androgen, androgen receptor action, anti-androgen enzalutamide, in vitro and in vivo models, imaging, gene fusions, and help facilitate studies with novel drugs including anti-androgens and chemotherapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Prostate Cancer: Methods and Protocols aims to provide easily reproducible techniques
    Pages: XI, 264 p. 45 illus., 40 illus. in color. : online resource.
    ISBN: 9781493978458
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  • 43
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Thyroid Hormone Receptors: Several Players for One Hormone and Multiple Functions -- Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin -- Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells -- In vitro Approaches to Identify Thyroid Hormone Receptor-dependent Transcriptional Response -- Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Phosphorylation -- Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Sumoylation -- Demonstration of the Receptor Site for Thyroid Hormone on Integrin αvß3 -- The Deiodinase Trio and Thyroid Hormone Signaling -- Thyroid Hormones and Derivatives – Endogenous Thyroid Hormones and their Targets -- Protocols for Studying how Thyroid Hormone Receptors Trigger Adaptive Thermogenesis in Mice -- Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells in vitro -- Analysis of Physiological Responses to Thyroid Hormones and their Receptors in Bone -- Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice -- Thyroid Hormone Action: The p43 Mitochondrial Pathway -- Evaluating thyroid Disrupting Chemicals in vivo using Xenopus laevis -- Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples -- Analysis of Thyroid Tumorigenesis in Xenograft Mouse Model -- Human Genetics Of Thyroid Hormone Receptor Beta: Resistance to thyroid Hormone Beta -- TRa Mutations in Human -- Role of Thyroid Hormone Receptor in Amphibian Development -- de novo Transcriptomic Approach To Study Thyroid Hormone Receptor Action in Non-mammalian Models -- Role of TRs in Zebrafish Development
    Abstract: This volume aims to detail the advances in the field of Thyroid hormones (THs), namely T3 and T4, and their nuclear receptor TRs from a conceptual and methodological point of view. Chapters guide readers through a general introduction, TR's engineered mice, human genetics of TRs, control of the hypothalamus-pituitary-thyroid (HPT) axis, the relevance of the local THs production and metabolism and pathological consequences of TH/TR alterations. Non-mammalian models are also presented and discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Thyroid Hormone Nuclear Receptor: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 300 p. 48 illus., 30 illus. in color. : online resource.
    ISBN: 9781493979028
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  • 44
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: CD36 Neuronal Identity in the Olfactory Epithelium -- Deorphanization of Olfactory Trace Amine-Associated Receptors -- G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction -- Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging -- Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates -- Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations -- Numerical Models and In Vitro Assays to Study Odorant Receptors -- High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling -- Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons -- In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats -- Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction -- Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ -- Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations -- Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice -- In Vivo Two-Photon Imaging of the Olfactory System in Insects -- Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder -- Methods in Rodent Chemosensory Cognition -- Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs -- Tracking Odorant Plumes -- Generative Biophysical Modeling of Dynamical Networks in the Olfactory System -- Behavioral Assays in the Study of Olfaction: A Practical Guide
    Abstract: This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques
    Pages: XI, 390 p. 66 illus., 47 illus. in color. : online resource.
    ISBN: 9781493986095
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  • 45
    Keywords: Medicine ; Biomedical Engineering ; Biotechnology ; Systems Biology ; Biomedicine ; Biomedical Engineering/Biotechnology ; Systems Biology ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Modeling: choosing a kinetics -- Chapter 3: Modeling at computer: getting started -- Chapter 4: Stochastic modeling -- Chapter 5: Steady states -- Chapter 6: Stability analysis: hysteresis and oscillations -- Chapter7: Toggle switch: dynamics and steady states -- Chapter 8: Rule-based modeling -- Chapter 9: Gene circuit modular computational design -- Chapter 10: Parameter analysis -- Chapter 11: Circuit analysis with COPASI -- Chapter 12: Circuit motifs
    Abstract: The textbook is based on the lectures of the course “Synthetic Biology” for Master’s students in biology and biotechnology at the Harbin Institute of Technology. The goal of the textbook is to explain how to make mathematical models of synthetic gene circuits that will, later on, drive the circuit implementation in the lab. Concepts such as kinetics, circuit dynamics and equilibria, stochastic and deterministic simulations, parameter analysis and optimization are presented. At the end of the textbook, a chapter contains a description of structural motifs (e.g. positive and negative feedback loops, Boolean gates) that carry out specific functions and can be combined into larger networks. Moreover, several chapters show how to build up (an analyse, where possible) models for synthetic gene circuits with four different open-source software i.e. COPASI, XPPAUT, BioNetGeN, and Parts & Pools-ProMoT
    Pages: XII, 187 p. 91 illus., 74 illus. in color. : online resource.
    ISBN: 9789811087523
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  • 46
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: The Past, Present, and Future of HLA Typing in Transplantation -- Role of Human Leukocyte Antigens (HLA) in Autoimmune Diseases -- The IPD Databases: Cataloguing and Understanding Allele Variants -- Allele Frequency Net Database -- High Resolution HLA-Typing by Next Generation Sequencing of Randomly Fragmented Target DNA -- High-Throughput Contiguous Full-Length Next Generation Sequencing of HLA Class I and II Genes from 96 Donors in a Single MiSeq Run -- Application of High-Throughput Next Generation Sequencing for HLA Typing on Buccal Extracted DNA -- Super High Resolution for Single Molecule-Sequence-Based Typing of Classical HLA loci using Ion Torrent PGM -- High Resolution Full-Length HLA Typing Method using Third Generation (Pac-Bio SMRT) Sequencing Technology -- Full-Length HLA Class I Genotyping with the MinION Nanopore Sequencer -- Imputation-Based HLA Typing with SNPs in GWAS Studies -- In Silico Typing of Classical and Non-Classical HLA Alleles from Standard RNA-Seq Reads -- PHLAT: Inference of High Resolution HLA Types from RNA and Whole Exome Sequencing -- Using Exome and Amplicon-Based Sequencing Data for High-Resolution HLA Typing with ATHLATES -- HLA Typing from Short-Read Sequencing Data with OptiType -- Comprehensive HLA Typing from a Current Allele Database using Next Generation Sequencing Data -- Accurate Assembly and Typing of HLA using a Graph-Guided Assembler Kourami -- AmpliSAS and AmpliHLA: Web Server Tools for MHC Typing of Non-Model Species and Human using NGS Data -- HLA Haplotype Frequency Estimation from Real-Life Data with the Hapl-o-Mat Software
    Abstract: This volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods. The chapters in this book discuss high-throughput methods for HLA typing; wet lab protocols; microarray data and its uses; in silico tools for the identification of HLA alleles from DNA and RNA next-generation-sequencing data, as well as HLA haplotype frequency estimation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, HLA Typing: Methods and Protocols is a valuable resource for any researcher interested in learning more about this developing field
    Pages: XI, 287 p. 52 illus., 38 illus. in color. : online resource.
    ISBN: 9781493985463
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  • 47
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Stereological Methods to Quantify Cell Loss in the Huntington’s Disease Human Brain -- Assessing Autophagic Activity and Aggregate Formation of Mutant Huntingtin in Mammalian Cells -- A Filter Retardation Assay Facilitates the Detection and Quantification of Heat-Stable, Amyloidogenic Mutant Huntingtin Aggregates in Complex Biosamples -- Cellular Models: HD Patient-Derived Pluripotent Stem Cells -- Non-Mammalian Models of Huntington’s Disease -- Mouse Models of Huntington’s Disease -- Motor Assessment in Huntington’s Disease Mice -- Automated Operant Assessments of Huntington’s Disease Mouse Models -- Neurophysiological Assessment of Huntington’s Disease Model Mice -- Murine Models of Huntington’s Disease for Evaluating Therapeutics -- Generating Excitotoxic Lesion Models of Huntington’s Disease -- Large-Brained Animal Models of Huntington’s Disease: Sheep -- Minipigs as Large-Brained Animal Model for Huntington’s Disease: From Behavior and Imaging to Gene Therapy -- Non-Human Primate Models of Huntington’s Disease and Their Application in Translational Research -- In Vivo Multidimensional Brain Imaging in Huntington’s Disease Animal Models -- Magnetic Resonance Imaging in Huntington’s Disease -- Biofluid Biomarkers in Huntington’s Disease -- Assessing and Modulating Kynurenine Pathway Dynamics in Huntington’s Disease: Focus on Kynurenine 3-Monooxygenase -- Assessing Mitochondrial Function in In Vitro and Ex Vivo Models of Huntington’s Disease -- Using Genomic Data to Find Disease-Modifying Loci in Huntington’s Disease (HD).-CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease -- Methods for Assessing DNA Repair and Repeat Expansion in Huntington’s Disease -- Translating Antisense Technology into a Treatment for Huntington’s Disease -- Disease Modification through Trophic Factor Delivery -- Methods to Quantify Cell Signaling and GPCR Receptor Ligand Bias: Characterization of Drugs that Target the Endocannabinoid Receptors in Huntington’s Disease -- Dissection and Preparation of Human Primary Fetal Ganglionic Eminence Tissue for Research and Clinical Application -- Robust Induction of DARPP32-Expressing GABAergic Striatal Neurons from Human Pluripotent Stem Cells -- Quality Assessment and Production of Human Cells for Clinical Use
    Abstract: This detailed book provides a laboratory manual and guidebook for the selection, implementation, and interpretation of a wide range of techniques in contemporary use in leading laboratories engaged in Huntington’s disease (HD) research worldwide. Only by understanding the pathology and pathogenic process at the fundamental molecular and cellular level can the research community expect to be able to slow or halt the disease process, repair the damage, and develop novel effective therapies to treat the symptoms of this condition, thus this volume collects the practical knowledge of its authors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Huntington’s Disease aims to help scientists to significantly extend the breadth and quality of research in laboratories dedicated to mastering and controlling this devastating human condition
    Pages: XV, 642 p. 111 illus., 74 illus. in color. : online resource.
    ISBN: 9781493978250
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  • 48
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Regulatory Guidelines on Stability Testing and Trending of Requirements -- The Stability Dossier: Common Deficiencies and Ways to Improve -- In Silico Drug Degradation Prediction -- Forced Degradation and Long-Term Stability Testing for Oral Drug Products: A Practical Approach -- A Model Approach for Developing Stability-Indicating Analytical Methods -- Protocols for Characterization of Degradation Products with Special Emphasis on Mutagenic Degradation Impurities -- Stability Studies: Facility and Systems -- User Requirements & Implementation of a Risk-Based, Compliant Stability Management System Based on the FDA New Guidance on DATA Integrity -- Stability Considerations in the Life Cycle of Generic Products -- Predictive Stability Testing Utilizing Accelerated Stability Assessment Program (ASAP) Studies -- Statistical Methods and Approaches to Avoid Stability Failures of Drug Product During Shelf-Life -- Estimation of Stability Based on Monitoring of Shipment and Storage -- Stability Testing Parameters and Issues for Nanotechnology-Based Drug Products -- Stability Testing Issues and Test Parameters for Herbal Medicinal Products -- Stability Testing Considerations for Biologicals and Biotechnology Products
    Abstract: This detailed volume collects numerous methods and protocols related to different aspects of stability programs that are followed in pharmaceutical development laboratories. Implementation of a successful stability program, vital in preventing product failures and recalls, requires critical and logical thinking that goes beyond the regular documented protocols and methods, so the experiences of the booḱ€™s internationally-based expert contributors fill the chapters with practical guidance. As a volume in the Methods in Pharmacology and Toxicology series, this book presents the kind of real-world advice that is essential for advancing laboratory research.℗ Authoritative and thorough, Methods for Stability Testing of Pharmaceuticals serves as a valuable addition to the existing armamentarium of resources available to stability testing personnel in research and industry
    Pages: XIV, 358 p. 93 illus., 42 illus. in color. : online resource.
    ISBN: 9781493976867
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  • 49
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Introduction to Epidemiological Studies -- Key Concepts in Genetic Epidemiology -- Quality Control of Common and Rare Variants -- Genome Wide Association Studies -- Assessing Rare Variation in Complex Traits -- Meta-analysis of Common and Rare Variants -- Gene-gene and Gene-environment Interactions -- Genetic Association in the HLA Region -- Novel Methods for Family-based Genetic Studies -- Methods for Polygenic Traits -- Multivariate Methods for Meta-analysis of Genetic Association Studies -- Methods of Analysis and Meta-Analysis for Identifying Differentially Expressed Genes -- A Primer in Mendelian Randomization Methodology with a Focus on Utilizing Published Summary Association Data -- Copy Number Variation -- From Identification to Function: Current Strategies to Prioritise and Follow-Up GWAS Results -- Translating Human Genetics into Novel Drug Targets
    Abstract: This volume details fast-moving research while providing in-depth descriptions of methods and analytical approaches that are helping to understand the genome and how it is related to complex diseases. Chapters guide the reader through common and rare variation, gene-gene and gene-environment interactions and state-of-the-art approaches for the synthesis of genome-wide and gene expression data. Novel approaches for associations in the HLA region, family-based designs, Mendelian Randomization and Copy Number Variation are also presented. The volume concludes with the challenges researchers face while moving from identifying variants to their functional role and potential drug targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a thorough presentation of methods and approaches and tips on troubleshooting and avoiding known pitfalls
    Pages: X, 299 p. 35 illus., 23 illus. in color. : online resource.
    ISBN: 9781493978687
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  • 50
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Immunotoxicology: A Brief History -- Clinical Immunotoxicology -- Investigative Immunotoxicology -- Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing -- Markers of Inflammation -- The Sheep Erythrocyte T-Dependent Antibody Response (TDAR) -- Methylated-Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice -- Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment -- Host Resistance Assays -- Enhanced Histopathology Evaluation of Lymphoid Organs -- Tumor Challenges in Immunotoxicity Testing -- Flow Cytometry for the Immunotoxicologist -- Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T Lymphocyte Assay -- Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing -- Natural Killer (NK) Cell Assays in Immunotoxicity Testing -- Dendritic Cell Assays -- Evaluating Macrophages in Immunotoxicity Testing -- Evaluating Cytokines in Immunotoxicity Testing -- Functional Assays of Hematopoietic Stem Cells in Toxicology Research -- CD4+ T Cell Differentiation and Activation -- Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing -- Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay -- Challenges for Integrating Immunotoxicology into the 21st Century Toxicology Testing Paradigm
    Abstract: This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing
    Pages: XIII, 412 p. 41 illus., 23 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493985494
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  • 51
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood -- EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma -- Cytoplasmic Immunoglobulin vs DNA Analysis by Flow Cytometry -- Deep Profiling of the Immune System of Multiple Myeloma Patients by Cytometry by Time of Flight (CyTOF) -- Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma -- Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYC -- RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine -- Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple Myeloma -- Analysis of Circulating Tumor DNA -- Detection of Cross-Sample Contamination in Multiple Myeloma Samples and Sequencing Data -- Analysis of Global Gene Expression Profiles -- Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing Protocol -- A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple Myeloma -- Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection -- Microfluidic Assembly of Liposomes with Tunable Size and Co-Loading Capabilities
    Abstract: This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients’ samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Multiple Myeloma: Methods and Protocols is a valuable resource for molecular scientists and laboratory supervisors working with MM samples, and any investigator conducting studies in this field
    Pages: X, 216 p. 41 illus., 32 illus. in color. : online resource.
    ISBN: 9781493978656
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  • 52
    Unknown
    Singapore : Springer Singapore
    Keywords: Medicine ; Pharmacology ; Nanochemistry ; Cell Biology ; Invertebrates ; Biomedicine ; Pharmacology/Toxicology ; Nanochemistry ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Values of C. elegans in Toxicological Study -- Endpoints for Toxicity Assessment of Nanomaterials -- Exposure Routes of Nanomaterials -- Toxic Effects of Certain Nanomaterials -- Physicochemical Basis for Nanotoxicity Formation -- Cellular and Physiological Mechanisms of Nanotoxicity Formation -- Molecular Mechanisms of Nanotoxicity Formation -- Distribution and Translocation of Nanomaterials -- Confirmation of Nanomaterials with Low-Toxicity or Non-Toxicity Property -- Surface Chemical Modification to Reduce the Toxicity of Nanomaterials -- Pharmacological Prevention of the Toxicity Induced by Environmental Nanomaterials
    Abstract: This book focuses on the toxicity of engineered nanomaterials (ENMs) and their underlying physicochemical, cellular, physiological, and molecular mechanisms. Further, it covers ENMs’ translocation and their targeted organ toxicology, and discusses chemical and pharmacological strategies used to combat nanotoxicity. Engineered nanomaterials (ENMs) are defined as materials with one or more dimensions of less than 100 nm, and have shown considerable promise in several areas of development. At the same time, the potential toxicity of ENMs for human health and environmental organisms is increasingly attracting attention. In addition to the typical properties of model animals, Caenorhabditis elegans is extremely sensitive to environmental toxicants, which makes it the ideal in vivo assay system for toxicological studies. C. elegans has been widely used in toxicity assessment and toxicological studies of environmental toxicants and stresses. This book provid