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  • 1
    Keywords: Cytology ; Neurosciences ; Cell Biology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System -- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing -- Myelin: Methods for Purification and Proteome Analysis -- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells -- Isolation and Culture of Oligodendrocytes -- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation -- Oligodendrocyte-Neuron Myelinating Co-Culture -- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier -- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices -- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord -- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination -- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination -- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation -- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis -- Conditional Mutagenesis in Oligodendrocyte Lineage Cells -- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage -- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination -- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease -- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes -- Transmission Electron Microscopy of Oligodendrocytes and Myelin -- Toxin-Based Models to Investigate Demyelination and Remyelination -- Magnetic Resonance techniques for Imaging White Matter
    Abstract: This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function
    Pages: XII, 411 p. 65 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990726
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  • 2
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Advancements in Managing Intracerebral Hemorrhage: Transition from Nihilism to Optimism -- Mandibular Advancement Devices in Patients with Symptoms of Obstructive Sleep Apnea: A Review -- Online Health Technologies and Mobile Devices: Attitudes, Needs and Future -- Radiological Response and Neutrophil-to-Lymphocyte Ratio as Predictive Factors for Progression-Free and Overall Survival in Metastatic Renal Cell Carcinoma Patients Treated with Sunitinib -- Plasminogen Activator Inhibitor Type 1 in Blood at Onset of Chemotherapy Unfavorably Affects Survival in Primary Ovarian Cancer -- Sialidase Attenuates Epidermal Growth Factor Response and Abolishes Antiproliferative Effects of Erlotinib in A549Alveolar Epithelial Cells -- Systemic Sclerosis and Serum Content of Transforming Growth Factor -- Protein-Bound Solute Clearance during Hemodialysis -- Mold Sensitization in Asthmatic and Non-Asthmatic Subjects Diagnosed with Extract-Based versus Component-Based Allergens -- Fructose Consumption and Lipid Metabolism in Obese Children and Adolescents -- Vaccination against Measles, Mumps, and Rubella in the Light of Current Epidemic Threats: Unjustified Postponement -- Variability of Dry-Eye Disease Following Removal of Lacrimal Glands in Rats
    Abstract: This book shares the latest research and practice-oriented findings in medical sciences with a wide audience. It addresses a range of contemporary issues, often unresolved or contentious, across various medical fields, including advances in the management of hemorrhagic brain stroke. It also discusses metastatic renal cell carcinoma – a global scourge with an extremely poor long-term survival prognosis, the course and sequelae of renal cell carcinoma, as well as advances in targeted molecular therapy with sunitinib, a receptor tyrosine kinase inhibitor. Further, it examines the molecular targeting of proliferative signaling of the epidermal growth factor receptor in the first-line treatment of patients with metastatic non-small-cell lung cancer. Other articles cover clearance of toxins in hemodialyzed patients; the search for diagnostic and therapeutic markers in the connective tissue disease scleroderma; obesity linked to inappropriate dietary habit; clinical problems related to the diagnosis of sensitization to fungi and its role in asthma; and reasons for the perilous trend of avoiding basic vaccinations in children. Lastly, the book explores the rapid developments in e-health technologies that increase access to health services, particularly for the elderly. The book is intended for clinical specialists, researchers, and all allied health professionals from various fields
    Pages: VI, 115 p. 17 illus., 10 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030190590
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  • 3
    Keywords: Microscopy ; Cytology ; Zoology ; Botany ; Biological Microscopy ; Popular Life Sciences ; Cell Biology ; Zoology ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Chapter 1:Introduction -- Chapter 2:The advantages of learning traditional microscopy -- Chapter 3:Buying a microscope -- Chapter 4:The science of light -- Chapter 5:Introduction to lenses -- Chapter 6:The anatomy of the microscope -- Chapter 7:The Eyepiece -- Chapter 8:The Objective -- Chapter 9:The Stage -- Chapter 10:The Condenser and Diaphragm -- Chapter 11:The Light source -- Chapter 12: Choosing the right technique -- Chapter 13:Sample prep -- Chapter 14:Image capture -- Chapter 15:Trouble-shooting
    Abstract: This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools
    Pages: XXI, 164 p. 51 illus., 43 illus. in color. : online resource.
    ISBN: 9783030053932
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  • 4
    Keywords: Medicine ; Nephrology ; Biochemistry ; Cytology ; Molecular Medicine ; Nephrology ; Biochemistry, general ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Prevalence and burden of chronic kidney disease -- Pathomorphism and evaluation of renal fibrosis -- Endothelial cells and renal fibrosis -- Mesangial cells and renal fibrosis -- Podocyte injury and glomerular sclerosis -- Tubular epithelial cell injury and renal fibrosis -- Monocyte-macrophages and renal fibrosis -- Pericytes and renal fibrosis -- Myofibroblast: Origin and regulation in renal fibrosis -- Mitochondrial lesion and renal fibrosis -- Metabolic nuclear receptors and renal fibrosis -- Role of renin angiotensin system in renal fibrosis -- Role of aldosterone in renal fibrosis -- TGF-β1 and renal fibrosis -- Connective tissue growth factor and renal fibrosis -- Lipid metabolism disorder and renal fibrosis -- Renal interstitial lymphangiogenesis in renal fibrosis -- Cell apoptosis and autophagy in renal fibrosis -- Pyroptosis and renal fibrosis -- Cell cycle arrest and renal fibrosis -- Inflammatory mediators and renal fibrosis -- Role of inflammasome in chronic kidney disease -- Oxidative stress and renal fibrosis -- Hypoxia and renal tubulointerstitial fibrosis -- Proteinuria and progression of chronic kidney disease -- Hypertension and renal fibrosis -- Diabetic Kidney disease and renal fibrosis -- Chronic allograft nephropathy and renal fibrosis -- Polycystic kidney disease and renal fibrosis -- Acute kidney injury and renal fibrosis -- Senescence and renal fibrosis -- Urinary biomarkers of renal fibrosis -- New therapies for the treatment of renal fibrosis
    Abstract: This book systemically presents the latest research on renal fibrosis, covering all the major topics in the field, including the possible mechanisms, biomarkers, and strategies for prevention and treatment of chronic kidney disease (CKD). Due to its high prevalence, CKD represents a huge global economic and social burden. Irrespective of the initial causes, CKD progresses to end stage kidney disease (ESKD) due to renal fibrosis, which is characterized by glomerulosclerosis, tubule atrophy and atresia, and the excessive accumulation of extracellular matrix (ECM) in the kidney. Unfortunately, an estimated 1%-2% of the adult population living with CKD will need renal replacement therapy at some point as a result of ESKD. As such, strategies for preventing or slowing CKD progression to ESKD are of utmost importance, and studies aiming to understand the mechanisms of renal fibrosis have been the focus of intensive research. Recently, novel insights into the pathophysiological processes have furthered our understanding of the pathogenesis of renal fibrosis, and more importantly, promoted studies on the early diagnosis and treatment of CKD. This book draws lessons from the extensive, state-of-the-art research in this field, elaborating the new theories and new techniques to offer readers a detailed and comprehensive understanding of renal fibrosis and as well as inspiration for future research directions
    Pages: XVI, 709 p. 63 illus., 55 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9789811388712
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  • 5
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Protocol to Compare Methods for Untargeted Metabolomics -- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry -- Measuring the Nutrient Metabolism of Adherent Cells in Culture -- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells -- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice -- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro -- Measuring Rates of ATP Synthesis -- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay -- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers -- Determining Compartment Specific Metabolic Fluxes -- Determining the Impact of Metabolic Nutrients on Autophagy -- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism -- Determining Macrophage Polarization upon Metabolic Perturbation -- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells -- Development of Patient-derived Tumor Xenograft Models -- Imaging Glioma Progression by Intravital Microscopy -- Lipectomizing Mice for Applications in Metabolism -- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants -- Determining the Intracellular Organization of Organelles -- The Fundamentals of Constructing and Interpreting Heat Maps
    Abstract: This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level
    Pages: XII, 294 p. 67 illus., 53 illus. in color. : online resource.
    ISBN: 9781493987696
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  • 6
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    Singapore : Springer
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Molecular Basis for Oxidative Stress Induced by Environmental Toxicants in Nematodes -- Molecular Basis for Reduced Lifespan Induced by Environmental Toxicants or Stresses -- Roles of Oxidative Stress Related Molecular Signals in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of MAPK Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Insulin and the Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Development Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Cell Death and DNA Damage Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Metabolism Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of Protective Response Related Signalling Pathways in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Functions of G-Protein Coupled Receptors and Ion Channels and the Downstream Cytoplasmic Signals in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Discussion on Specificity of Molecular Signals in Response to Certain Environmental Toxicants or Stresses -- Epigenetic Regulation of Toxicity of Environmental Toxicants or Stresses -- Strategies to Screen and to Identify New Genetic Loci Involved in the Regulation of Toxicity of Environmental Toxicants or Stresses -- Molecular Basis for Adaptive Response to Environmental Toxicants or Stresses -- Molecular Basis for Transgenerational Toxicity Induction of Environmental Toxicants or Stresses
    Abstract: This book will focus on the molecular basis of oxidative stress induced by toxicants or stresses and various molecular signalling pathways in regulating the toxicity of toxicants or stresses in Caenorhabditis elegans. It will also cover the discussion on the aspects of response signals, G-protein coupled receptors and ion channels, specific molecular signals, and epigenetic signals involved in the regulation of toxicity from toxicants or stresses. The molecular basis for adaptive response for transgenerational toxicity of environmental toxicants or stresses will be further discussed. Nematode Caenorhabditis elegans is a classic model animal with well-described genetic and developmental backgrounds based on the study of life science, and has been further successfully and widely used in both toxicity assessment and toxicological study of various environmental toxicants or stresses. Based on related available data, this book aims at providing a systematic understanding of the knowledge system of molecular toxicology in C. elegans
    Pages: XVIII, 447 p. 285 illus., 211 illus. in color. : online resource.
    ISBN: 9789811336331
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  • 7
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Left Ventricular Strain and Relaxation are Independently Associated with Renal Cortical Perfusion in Hypertensive Patients -- Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: a Bispectral Analysis -- Influence of Heart Rate, Age, and Gender on Heart Rate Variability in Adolescents and Young Adults -- Hand-Foot Syndrome and Progression-Free Survival in Patients Treated with Sunitinib for Metastatic Clear Cell Renal Cell Carcinoma -- Elastography in the Diagnosis of Pancreatic Malignancies -- Factors Affecting Health-Related Quality of Life in Liver Transplant Patients -- Phagocytosis and Autophagy in THP-1 cells Exposed to Urban Dust: Possible Role of LC3€“Ássociated Phagocytosis and Canonical Autophagy -- Gendeŕ€“Dependent Responses of Growth and Insuliń€“like Growth Factoŕ€“1 to Growth Hormone Therapy in Prepubertal Growth Hormone Deficient Children -- Vitamin D and Calcium Homeostasis in Infants with Urolithiasis -- Morphometric Analysis of the Lumbar Vertebrae Concerning the Optimal Screw Selection for Transpedicular Stabilization -- Stress and Dehumanizing Behaviors of Medical Staff toward Patients -- Biological and Social Determinants of Maximum Oxygen Uptake in Adult Men
    Abstract: This book focuses on issues in clinical practice and research that are of general interest. The articles primarily focus on understanding the pathogenic mechanisms of diseases, their prevention, and therapy. The topics addressed include cardiovascular regulation with regard to blood pressure and heart rate variability, and to coupling blood pressure changes with subarachnoid fluid oscillations. In addition, the book discusses recent advances in the diagnostics of and targeted molecular therapy for renal and pancreatic malignancies, growth disorders, vitamin D and calcium homeostasis in children in the context of neonatal urolithiasis, and neurosurgical interventions in multifarious age-related diseases of the vertebrae. Neuropsychological aspects of patients’ quality of life and of shaping medical staff’s attitude toward patients are also addressed. The respective articles are intended to build a bridge between basic and clinical research. Further, the book enhances the current body of knowledge on diagnostics and patient treatment and offers valuable new perspectives on practical clinical issues. As such, it offers a unique resource for clinicians, family physicians, medical scholars, and professionals engaged in patient management
    Pages: VI, 114 p. 20 illus. in color. : online resource.
    ISBN: 9783030129231
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  • 8
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 9
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Setting Up a Single-Cell Genomic Laboratory -- Tissue Handling and Dissociation for Single-Cell RNA-seq -- Full-Length Single-Cell RNA-Sequencing with Smart-seq2 -- CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification -- Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq) -- Single-Cell RNA Sequencing with Drop-Seq -- Chromium 10X Single Cell 3’ mRNA Sequencing of Tumor Infiltrating Lymphocytes -- Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-Sequencing -- Single-Cell Tagged Reverse Transcription (STRT-seq) -- Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ -- High Throughput Single Cell Real-Time Quantitative PCR Analysis -- Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ System -- Targeted TCR Amplification from Single-Cell cDNA Libraries -- Sequencing the Genomes of Single Cells -- Studying DNA Methylation in Single Cell Format with scBS-seq -- Single Cell 5fC Sequencing -- ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions -- Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry -- Classification of the Immune Composition in the Tumor Infiltrate -- Combined Genome and Transcriptome (G&T) Sequencing of Single Cells -- Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell -- Simultaneous Targeted Detection of Proteins and RNAs in Single Cells -- CRISPR-Screening in Single Cells -- Single Cell Live Imaging -- Differential Expression Analysis in Single Cell Transcriptomics -- A Bioinformatic Toolkit for Single-Cell mRNA Analysis
    Abstract: This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab
    Pages: XVI, 459 p. 87 illus., 78 illus. in color. : online resource.
    ISBN: 9781493992409
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  • 10
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Role of Redox in Signal Transduction -- Methods for the Addition of Redox Compounds -- Investigating ROS, RNS, and H2S Sensitive Signalling Proteins -- Measurement of 4-hydroxynonenal (4-HNE) Protein Adducts by ELISA -- Using Flow Cytometry to Detect and Measure Intracellular Thiol Redox Status in Viable T Cells from Heterogeneous Populations -- Detection of S-Nitrosation and S-Glutathionylation of the Human Branched-Chain Aminotransferase Proteins -- Visualization of Intracellular Hydrogen Peroxide produced by DAO with Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells -- Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy to the Study of Oxidative Stress in Biological Systems -- Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins -- Working with Hypoxia -- Predicting the Effects of Low Dose-Rate Ionising Radiation on Redox Potential in Plant Cells -- Thioredoxin-1 PEGyletion as an In Vitro Method for Drug Target Identification -- Redox-Regulated, Targeted Affinity Isolation of NADH-Dependent Protein Interactions with the Branched Chain Aminotransferase Proteins -- Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures -- Equations to Support Redox Experimentation
    Abstract: This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen sulphide in cell signaling; measuring modifications using Flow Cytometry, ELISA assays, and Western blot analysis; measurement of oxidative stress in mitochondira and biological systems; and the use of the genetically encoded fluorescent probe HyPer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field
    Pages: X, 199 p. 34 illus., 15 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493994632
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  • 11
    Keywords: Biochemistry ; Cytology ; Oncology ; Biochemistry, general ; Cell Biology ; Cancer Research ; Springer Nature Living Reference
    Description / Table of Contents: A. Analytical and Structural Approach - Technical Description for Analyzing Glycan Structures (Reviewed by Yoshiki Yamaguchi): Overview -- EMARS -- Development of glycan array -- Development of glycan array -- Development of glycan array -- Development of glycan array -- Automated programmable one-pot synthesis of glycans -- Lectin array -- Lectin-glycan interaction based on glycan array data -- Glycoform-specific protein staining by lectin and antibody -- Glycosaminoglycans analysis -- Mass analysis -- Mass analysis -- IGOT-lectin capture -- Membrane electrophoresis -- HPLC nucleotide-sugar analysis -- Capillary electrophoresis -- HPLC PA-sugar -- Congenital disorders of glycosylation, analytical aspect -- Quality control of pharmaceutical products and biosimilars -- NMR -- NMR -- STD-NMR -- Conformational analysis of glycans and glycoproteins -- Glycolipid LC-MS -- MALDI-MS -- MALDI-MS -- Sulfoglycolipids -- GAG array -- X-ray -- REMD simulation -- MD simulation and computational screening -- B. Glycoinformatics - New Approach for Informatics and Databases for Glycoscience (Reviewed by Kiyoko F. Aoki-Kinoshita): Informatics overview -- MIRAGE project -- RINGS -- JCGGDB -- UniCarb-DB -- CIRES -- GLYCOSCIENCES.de -- CSDB, Plant and Bacterial Carbohydrate Structure Database -- MonosaccharideDB -- GlycomeDB -- GlycoEpitope -- SugarBindDB -- GLYCAM -- CAZy -- 3D Lectines -- C. Chemoenzymatic Synthesis of Glycans - Chemical and Enzymatic Methods of Glycan Synthesis (Reviewed by Yasuhiro Kajihara and Peter Seeberger): Overview -- Chemical synthesis of glycans -- Chemical synthesis of glycans -- Chemical synthesis of glycans -- Synthesis of homogeneous glycoproteins -- Synthesis of homogeneous glycoproteins -- Synthesis of homogeneous glycoproteins -- Synthesis of homogeneous glycoproteins -- Synthesis of homogeneous glycoproteins -- Glycopeptide/glycoprotein synthesis -- Gangliosides synthesis -- Specific Glycosylation -- Furanoside chemistry -- Glycoside synthesis -- Carbohydrate Library Synthesis -- Development of sugar array -- Development of sugar array -- Development of sugar array -- Automated synthesis -- Automation in glycan synthesis -- GPI chemical synthesis -- Oligosialic acid synthesis -- Synthesis of sulfated glycans -- Synthesis of sulfated glycans -- Synthetic antitumor vaccines -- Synthetic carbohydrate antigens for HIV Vaccine Design -- Chemical approach in biology and disease -- Glycoenzyme inhibitors -- Glycoenzyme inhibitors -- Glycoenzymes in glycan analysis and synthesis -- Endo-enzymes -- Oxazoline derivatives -- Large-scale enzymatic synthesis of glycans with cofactor regeneration -- Large-scale enzymatic synthesis of glycans -- Chemoenzymatic synthesis -- Chemoenzymatic synthesis of heparins -- Chemoenzymatic synthesis of glycoproteins -- Multivalent glycan synthesis -- Biosynthesis of A and B blood group -- Glycosyltransferase structures -- Structural biology of oligosaccharyltransferase (OST) -- Chemoenzymatic synthesis of oligosaccharides and cancer and bacterial vaccines -- Neoglycoprotein and oligosaccharide synthesis -- Chemoenzymatic synthesis of glycoconjugates -- Synthesis of O-glycosylated proteins -- D. Imaging of Glycans - New Techniques for Imaging Glycans (Reviewed by Yasuhiro Kajihara and Chi-Huey Wong): Molecular probes for glycosylation: overview Imaging by Click Chemistry -- Imaging by Click Chemistry -- Imaging by Click Chemistry -- Chemical tools to detect Helicobacter pylori -- Enzymatic imaging -- PET imaging -- Glycoprotein imaging -- E. Neuroglycobiology - The Role of Glycans in Neurobiology and Neuroscience (Reviewed by Kenji Kadomatsu): Neuroglycobiology overview -- Neurochemistry and developmental neurobiology -- Glycans in neurobiology -- Glycosaminoglycans (GAGs) -- GAGs -- Polysialic acid -- Polysialic acid -- Glycolipids sialidase -- HNK-1 -- Ganglioside -- Single molecule imaging -- Glycolipid -- Heparan sulfate proteoglycans (HSPG) -- HSPG -- N-glycans and glial cells
    Abstract: The aim of the book is to provide a succinct overview of the current status of glycoscience from both basic biological and medical points of view and to propose future directions, in order to facilitate further integrations of glycoscience with other fields in biological and medical studies. Glycans (carbohydrate oligomers) are the so-called “building blocks” of carbohydrates, nucleic acids, proteins and lipids and play major roles in many biological phenomena as well as in various pathophysiological processes. However, this area of glycoscience has been neglected from the research community because glycan structures are very complex and functionally diverse and as compared to proteins and nucleic acids simple tools for the amplification, sequencing and auto-synthesis of glycans are not available. Many scientists in other fields of research have now realized that glycosylation, i.e. the addition of glycans to a protein backbone, is the most abundant post translational modification reactions and is an important field of research and sometimes they require a glycobiology and/or glycochemistry approach to be used. It is still difficult, however, for non-expert researchers to use these techniques. This book will provide numerous but simple overviews of current topics and protocols for the experiments. The book is aimed at university students and above, including non-experts in the field of glycoscience
    Pages: Approx. 1200 p. 540 illus., 240 illus. in color. : online resource.
    ISBN: 9784431548362
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  • 12
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    Dordrecht : Springer
    Keywords: Medicine ; Cytology ; Diabetes ; Bioorganic chemistry ; Human Physiology ; Biomedicine general ; Cell Biology ; Diabetes ; Science, Humanities and Social Sciences, multidisciplinary ; Bioorganic chemistry ; Human Physiology ; Springer Nature Living Reference
    Description / Table of Contents: Microscopic Anatomy of the Human Islet of Langerhans -- The Comparative Anatomy of Islets -- Approaches for Imaging Islets: Recent Advances and Future Prospects -- Islet Cell Development -- High Fat Programming of β-Cell Failure -- Electrophysiology of Islet Cells -- ATP-Sensitive Potassium Channels in Health and Disease -- Role of Mitochondria in β-cell Function and Dysfunction -- Basement Membrane in Pancreatic Islet Function -- Calcium Signaling in the Islets -- Electrical Bursting, Calcium Oscillations, and Synchronization of Pancreatic Islets -- Exocytosis in Islet β-Cells -- Proteomics and Islet Research -- Wnt Signaling in Pancreatic Islets -- Mechanisms of Pancreatic β-Cell Apoptosis in Diabetes and Its Therapies -- β-Cell Function in Obese-Hyperglycemic Mice [ob/ob Mice] -- Islet Structure and Function in the GK Rat -- The β-Cell in Human Type 2 Diabetes -- Clinical Approaches to Preserve β-Cell Function in Diabetes -- Immunology of β-Cell Destruction -- Prevention of β-Cell Destruction in Autoimmune Diabetes: Current Approaches and Future Prospects -- Islet Isolation for Clinical Transplantation -- Human Islet Autotransplantation: The Trail Thus Far and the Highway Ahead -- Modulation of Early Inflammatory Reactions to Promote -- Successes and Disappointments with Clinical Islet Transplantation -- Exercise-induced pancreatic islets adaptations in health and disease -- Role Reg family proteins on islet cell growth and survival -- Circadian control of islet function -- Zinc Transporters in the pancreas -- Making Islets from Human Embryonic Stem Cells -- Chloride channels and Transporters in ß-cell physiology -- Pancreatic beta cells in metabolic syndrome -- Insulin and IGF-1 receptor signalling in insulin secreting cells -- Beta cell store-operated ion channels -- Isolation of Rodent Islets of Langerhans -- Anionic Transporters and Channels in Pancreatic Islet Cells -- Glucose-Induced Apoptosis in Pancreatics Islets -- In Vivo Biomarkers for Detection of Beta Cell Death -- Inflammatory Pathways Linked to Beta Cell Demise in Diabetes -- Islet Encapsulation -- Islet Isolation for Autotransplantation -- Islet Xenotransplantation: recent advances and future prospects -- Molecular basis of cAMP signaling in pancreatic beta cells -- NADPH Oxidase in Beta Cell Dysfunction -- Pancreatic Neuroendocrine Tumors -- Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic Alpha-Cells -- Reproductive Hormones and Islet Adaptation to Metabolic Stress -- Stem Cells in Pancreatic Islets -- Transcriptional co-Activators in Beta-Cell Function and Disease Pathology
    Abstract: This book contains critical background information, and recent advances made in essentially all areas of islet research. It is a major reference book, the first of its kind, for islet researchers, and diabetes researchers. Anybody, including the experts, and the beginners, interested in the study of islet physiology, and diabetes, will find this book extremely useful. The book is robust in its breadth: it deals with anatomy, histology, ultra-structure, evolution and comparative anatomy, imaging, developmental biology, programming, apoptosis, mitochondrial function, metabolism, cellular signaling, electrophysiology, oscillation of hormone secretion, islets of model animals, immunology, proteomics, regenerative medicine, clinical advances, and islet transplantation. Individual chapters contributed by a large number of experts and enthusiasts, not only provide a balanced view of the recent advances made in the respective fields, but also provide directions and thoughts for future research. Thanks to vivid and colorful illustrations, tables and sketches, the book as a whole, and the individual chapters make reading a pleasant experience. If you are interested in diabetes research, you will love to have a personal copy of this book
    Pages: 800 p. 240 illus., 200 illus. in color. : online resource.
    ISBN: 9789400768840
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  • 13
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Increased Risk of Lung Metastases in Patients with Giant Cell Bone Tumors: A Systematic Review -- Acceptance of Illness Associates with Better Quality of Life in Patients with Non-malignant Pulmonary Diseases -- Heart Rate Variability in the Diagnostics and CPAP Treatment of Obstructive Sleep Apnea -- Subterranean Pulmonary Rehabilitation in Chronic Obstructive Pulmonary Disease -- Epidemiological Aspects of Low Back Pain -- Sex Hormones Response to Physical Hyperoxic and Hyperbaric Stress in Male Scuba Divers: a Pilot Study -- Cigarette Smoke-induced Oxidative Stress and Autophagy in Human Alveolar Epithelial Cell Line (A549 Cells) -- Relative Cerebral Blood Transit Time Decline and Neurological Improvement in Patients after Internal Carotid Artery Stenting -- Influence of Glycemic Control and Coagulation and Lipid Metabolism in Pregnancies Complicated by Pregestational and Gestational Diabetes Mellitus -- Maternal Nutritional and Water Homeostasis as a Presage of Fetal Birth Weight -- Cytotoxicity, Oxidative Stress and Autophagy in Human Alveolar Epithelial Cell Line (A549 Cells) Exposed to Standardized Urban Dust -- Autologous Platelet-Rich Plasma Reduces Healing Time of Chronic Venous Leg Ulcers: A Prospective Observational Study
    Abstract: This book gathers multidisciplinary articles that present advances of our understanding of diseases and the effective treatment of patients. The authors share recent clinical and experimental research findings, highlighting poorly understood areas with uncertain treatment outcomes, such as giant-cell bone tumors and their propensity to metastasize to the lungs; subterranean rehabilitation in pulmonary disorders; male reproductive hormone regulation during physical exercise in hyperbaric, hyperoxic environments, like underwater diving; and amelioration of cognitive decline owing to increased cerebral blood transit time after internal carotid artery stenting. Other topics include new concepts and innovations in the treatment of diabetes in pregnancy, and leg ulcers in chronic venous insufficiency, as well as molecular research on the toxic effects of oxidative stress, impaired cell autophagy, and experimental conditions resembling air pollution. Featuring the latest interdisciplinary advances in biomedicine, this book is a valuable resource for medical professionals, both academics and practitioners, and all allied health-care workers
    Pages: VI, 118 p. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030253738
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  • 14
    Keywords: Microbiology ; Cytology ; Evolution (Biology) ; Biochemistry ; Eukaryotic Microbiology ; Cell Biology ; Evolutionary Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: The evolution of oxygen independent energy metabolism in eukaryotes with hydrogenosomes and mitosomes -- Chapter 3: Protein Import into Hydrogenosomes and Mitosomes -- Chapter 4: Structure of the Hydrogenosome -- Chapter 5: Hydrogenosomes of Anaerobic Ciliates -- Chapter 6: Metabolism of Trichomonad Hydrogenosomes -- Chapter 7: Hydrogenosomes of Anaerobic Fungi: an Alternative Way to Adapt to Anaerobic Environments -- Chapter 8: The proteome of T. vaginalis hydrogenosomes -- Chapter 9: Mitosomes in parasitic protists -- Chapter 10: The Mitochondrion-Related Organelles of Crypto-sporidium species -- Chapter 11: The Mitochondrion-Related Organelles of Blastocystis -- Chapter 12: Mitochondrion-related organelles in free-living protists -- Chapter 13: Protists without mitochondria, how it may happen?
    Abstract: "Hydrogenosomes and Mitosomes: Mitochondria of Anaerobic Eukaryotes, 2nd edition" provides a comprehensive summary of the current knowledge on these organelles, which occur in unicellular, often parasitic organisms, including human pathogens. It discusses the discovery of these widely distributed organelles, as well as their evolution and recent advances in the study of their structure and function. The book also describes their properties, such as protein import, structure, metabolism and adaptation, their proteome and their role in drug activation and resistance. The book will appeal to researchers and students interested in biology and medicine, and to those who are mainly interested in basic science-cell biology, parasitology, microbiology, evolution etc., but also to those interested in organelles as potential targets for chemotherapy
    Pages: VIII, 326 p. 52 illus., 17 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030179410
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  • 15
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) -- Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells -- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD -- Chromatin Immunoprecipitation in Skeletal Myoblasts -- Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor -- Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation -- Lentivirus-Mediated RNAi in Skeletal Myogenesis -- Adipogenesis from Bovine Precursors -- Transcriptomic Profiling during Myogenesis -- Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle -- Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System -- LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue -- siRNA Gene Silencing during C2C12 Myogenesis -- Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury -- Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis -- Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis -- Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers -- Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis -- Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies -- RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis
    Abstract: This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis
    Pages: XII, 351 p. 84 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988976
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  • 16
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility -- Isolation of Human Peripheral Blood T Lymphocytes -- A Laboratory Model to Study T-Cell Motility -- Quantification of T-Cell Migratory Phenotypes using High Content Analysis -- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time -- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells -- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis -- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes -- GapmeR-Mediated Gene Silencing in Motile T-Cells -- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy -- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes -- Immunometabolomic Phenotyping of Motile T-Cells -- Profiling Activity of Cellular Kinases in Migrating T-Cells -- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes -- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy -- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides -- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility -- Computational Analysis of Protein-Protein Interactions in Motile T-Cells
    Abstract: This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging
    Pages: X, 158 p. 31 illus., 28 illus. in color. : online resource.
    ISBN: 9781493990368
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  • 17
    facet.materialart.
    Cham : Springer
    Keywords: Biological models ; Human Genetics ; Endocrinology ; Cytology ; Systems Biology ; Human Genetics ; Endocrinology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: 1. History of the thymus: From a vestigial organ to the programming of immunological self-tolerance -- 2. Thymus Ontogeny and Development -- 3. The Ins and Outs of Thymic Epithelial Cell Differentiation and Function -- 4. T-Cell Development: From T-Lineage Specification to Intrathymic Maturation -- 5. Intrathymic cell migration: implications in thymocyte development and T-cell repertoire formation -- 6. Thymic crosstalk: an overview of the complex cellular interactions that control the establishment of T-cell tolerance -- 7. The Autoimmune Regulator (AIRE) gene, the master activator of self-antigen expression in the thymus -- 8. Aire Mutations and Autoimmune Diseases -- 9. The thymus as a mirror of the body's gene expression -- 10. Functional genomics of the infant human thymus: Aire and minipuberty -- 11. Thymus transcriptome of TGF-β superfamily -- 12. Development of thymic regulatory T lymphocytes -- 13. Adding insult to injury: improving the regenerative capacity of the aged thymus following clinically induced damage -- 14. Thymus rejuvenation after autologous hematopoietic stem cell transplantation in patients with autoimmune diseases
    Abstract: This volume focuses on challenging field in biomedicine that is the genetic control of central immune tolerance. It covers the thymus development, their cellular components and their respective function, the peculiar gene expression profiling (transcriptome) found in the medullary thymic epithelial cells (mTECs) that are implicated in the self-representation in the thymus, the Autoimmune regulator (Aire) gene, the mutations in this gene and manifestation of autoimmune diseases, and the role of cell-cell interactions within the thymus with implications in the negative selection (elimination) of nascent autoreactive T cells in preventing aggressive autoimmunity. The thymus gland is a lymphoid organ implicated in the maturation, differentiation and selection of T cells. This organ is gained more and more attention in different biomedical research labs worldwide due to its function that is associated with the control of immune homeostasis in the body, establishing the central immune tolerance and preventing the onset of autoimmune diseases
    Pages: XVII, 318 p. 30 illus., 23 illus. in color. : online resource.
    ISBN: 9783030120405
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  • 18
    Keywords: Biochemistry ; Cytology / Research ; Cytology ; Genetics ; Biochemistry, general ; Biological Techniques ; Medical Biochemistry ; Cell Biology ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Ionic and Covalent Bonds -- Introduction to Biological Macromolecules -- Carbohydrates -- Enzyme Kinetics
    Abstract: This textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The “Labster Virtual Lab Experiments” series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn’t have access to. In this book, you’ll learn the fundamental concepts of basic biochemistry focusing on: Ionic and Covalent Bonds Introduction to Biological Macromolecules Carbohydrates Enzyme Kinetics In each chapter, you’ll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you’ll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access to five simulations for the duration of six months. If you’re using the e-book version, you can sign up and buy access to the simulations at www.labster.com/springer. If you like this book, try out other topics in this series, including “Basic Biology”, “Basic Genetics”, and “Genetics of Human Diseases”. About Labster Labster is a company dedicated to developing virtual lab simulations that are designed to stimulate students’ natural curiosity and highlight the connection between science and the real world. These simulations have been shown to improve the achievement of learning outcomes among students, by making the learning experience more immersive and engaging. The content of this book was created by the Labster team members Dr. Aaron Gardner, Dr. Wilko Duprez, Dewi Ayu Kencana Ungu, Dr. Sarah Stauffer, Dr. Frederik Clauson-Kaas, and Silvia Tjong. You can learn more about Labster and explore the simulations at www.labster.com
    Pages: IX, 87 p. 47 illus. : online resource.
    ISBN: 9783662584996
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  • 19
    Keywords: Biochemistry ; Cytology ; Cell receptors ; Protein Science ; Cell Biology ; Receptors ; Springer eBooks
    Description / Table of Contents: A Brief History of the b-Arrestins -- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling -- Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET -- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle -- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer -- Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET -- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin -- Methods to Investigate Arrestins in Complex with Phosphodiesterases -- Methods to Characterize Protein Interactions with β-arrestin in cellulo -- Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling -- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing -- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners -- Workflow Description to Dynamically Model β-Arrestin Signaling Networks -- Proteomic Analysis of the β-Arrestin Interactome -- Quantitating Ligand Bias using the Competitive Model of Ligand Activity -- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins -- Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy -- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins -- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors -- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling -- Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease -- Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function -- Methods to Investigate β-Arrestin Function in Metabolic Regulation -- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease -- Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells
    Abstract: This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins
    Pages: XIV, 409 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493991587
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  • 20
    Keywords: Cytology ; Mass spectrometry ; Monoclonal antibodies ; Cell Biology ; Mass spectrometry ; Antibodies ; Springer eBooks
    Description / Table of Contents: Setting up Mass Cytometry in a Shared Resource Lab Environment -- Acquisition, Processing, and Quality Control of Mass Cytometry Data -- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating -- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments -- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis -- Titration of Mass Cytometry Reagents -- Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry -- Automated Cell Processing for Mass Cytometry Experiments -- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry -- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry -- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry -- Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry -- Mass Cytometric Cell Cycle Analysis -- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry -- Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry -- Data-Driven Flow Cytometry Analysis -- Analysis of Mass Cytometry Data -- Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry -- Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples -- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery
    Abstract: This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 338 p. 83 illus., 70 illus. in color. : online resource.
    ISBN: 9781493994540
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  • 21
    Keywords: Developmental Biology ; Cytology ; Developmental Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The neural crest: a remarkable model system for studying development and disease -- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example -- Generation of neural crest progenitors from human pluripotent stem cells -- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling -- Deciphering the neural crest contribution to cephalic development with avian embryos -- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis -- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants -- Technologies for live imaging of enteric neural crest derived cells -- Mouse embryo culture for the study of neural crest cells -- Laser capture microdissection of murine embryonic neural crest cells -- In vivo and in vitro quantitative analysis of neural crest cell migration -- Proteomics in neural crest cell research -- Neural crest methodologies in zebrafish and medaka -- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells -- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity -- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective
    Abstract: This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike
    Pages: X, 230 p. 36 illus. in color. : online resource.
    ISBN: 9781493994120
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  • 22
    Keywords: Oncology ; Human Genetics ; Medicine ; Cytology ; Metabolism ; Cancer Research ; Human Genetics ; Molecular Medicine ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Preface -- Molecular and Cell Biology of Cancer when cells break the rules and hijack their own planet -- Cancer as an Evolutionary Process -- Cell signaling in cancer -- The cell cycle, cytoskeleton and cancer -- Genomic Instability: DNA Repair and Cancer -- Cell metabolism in cancer – an energetic switch -- Cancer immunoediting and hijacking of the immune system -- Angiogenesis - vessels recruitment by tumor cells -- Tumor niche disruption and metastasis: the role of epithelial-mesenchymal transition (EMT) -- Case studies - Molecular Pathology perspective and impact on oncologic patients’ management -- Index
    Abstract: This textbook takes you on a journey to the basic concepts of cancer biology. It combines developmental, evolutionary and cell biology perspectives, to then wrap-up with an integrated clinical approach. The book starts with an introductory chapter, looking at cancer in a nut shell. The subsequent chapters are detailed and the idea of cancer as a mass of somatic cells undergoing a micro-evolutionary Darwinian process is explored. Further, the main Hanahan and Weinberg “Hallmarks of Cancer” are revisited. In most chapters, the fundamental experiments that led to key concepts, connecting basic biology and biomedicine are highlighted. In the book’s closing section all of these concepts are integrated in clinical studies, where molecular diagnosis as well as the various classical and modern therapeutic strategies are addressed. The book is written in an easy-to-read language, like a one-on-one conversation between the writer and the reader, without compromising the scientific accuracy. Therefore, this book is suited not only for advanced undergraduates and master students but also for patients or curious lay people looking for a further understanding of this shattering disease
    Pages: IX, 216 p. 71 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030118129
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  • 23
    Keywords: Oncology ; Human Genetics ; Medicine ; Cytology ; Cancer Research ; Human Genetics ; Molecular Medicine ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Preface -- Hypoxia mediates tumor malignancy -- Clinical methods for quanitifying hypoxia in human tumors -- Hypoxia-induced phenotypes that mediate tumor heterogeneity -- Hypoxia and the tumors secretome -- Hypoxic signaling in angiogenesis and lymphangiogenesis -- Hypoxia induced metabolic reprogramming in cancer metastasis -- The metastatic niche and hypoxia -- Hypoxia and immune suppression -- Hypoxia-induced resistance to cancer therapy -- Therapeutic stratagies to target the hypoxic microenvironment -- Index
    Abstract: The present book is an attempt to provide a detailed review of studies that clarify our current understanding of the role of hypoxia in the progression of primary cancer to metastatic disease. It will enable researchers to discover the critical cellular changes that occur under hypoxic conditions and play a role in metastatic dissemination, from the activation of hypoxia-inducible factors, HIF-1 and HIF-2, to the transcriptional profile changes that occur in cancer cells and promote cancer cell survival under detrimental conditions. Readers will discover the methods and challenges involved in imaging and quantifying the degree of hypoxia in a primary tumor. We will provide an understanding of the hypoxia-induced phenotypes that influence heterogeneity, alter the secretome and tumor microenvironment, modify cellular metabolism, and promote immune suppression and resistance to chemotherapy. Finally, we will uncover the therapeutic strategies that are being devised to target the hypoxic microenvironment in the hopes of preventing metastasis and improving the efficacy of standard-of-care cancer treatments. This work is an up to date source of information on the challenges and complexity of the hypoxic tumor microenvironment. Basic and translational scientists, post-doctoral fellows, graduate students, and those interested in how tumors metastasize will find this book a reference that details how hypoxia influences metastatic disease
    Pages: VIII, 160 p. 17 illus., 16 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030127343
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  • 24
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    New York, NY : Springer
    Keywords: Medical genetics ; Medicine ; Cytokines ; Cytology ; RNA-ligand interactions ; Bioinformatics ; Gene Function ; Molecular Medicine ; Cytokines and Growth Factors ; Cell Biology ; Protein-Ligand Interactions ; Bioinformatics ; Springer Nature Living Reference
    Description / Table of Contents: 5-Hydrocytryptamine receptor 3a -- 5-Hydroxytryptamine receptor 2B -- 5-Hydroxytryptamine receptor 2C -- A-Raf -- ABCA -- Ack1 -- ADAP -- Adap1 -- Adenylyl cyclase -- Adenylyl cyclase type 9 -- Adhesion-GPCRs -- ADP-ribosylation factor domain protein 1 -- Ags (Activators of G-protein signaling) -- Aif -- AKAP -- Androgen receptor -- Angiotensin II receptor 2 -- Ant -- AP-3 -- AP-4 -- Apo2L (TRAIL) -- Arap3 -- Arf-like protein 8b -- Arfgap1 -- Arfrp1 -- ATF3 -- Aurora kinase -- Bcl2 family -- Beta-Catenin -- Bradykinin receptor -- Btg/Tob -- c-Myb -- Cadherin -- Calcium channel, voltage-dependent -- Calpain -- Carma1 -- Casein kinase II -- Caspase -- Cbln1 -- CCL3 [chemokine (C-C motif) ligand 3] -- CCL4 [chemokine (C-C motif) ligand 4] -- CCL5 [chemokine (C-C motif) ligand 5] -- CCN -- CCTa -- CD160 -- CD3 -- CD3 zeta -- CD38 -- CD40 -- CD43 -- CD45 -- CD47 -- CD53 -- CD72 -- CD81 -- CD91 -- Cdc7 -- CDK11 -- Ceacam -- Chemokine ligand CX3CL1 -- Chemokine receptor CCR1 -- Cholecystokinin type B receptor (CCK type B receptor) -- Cholecystokinin-1 receptor -- CKIP-1 -- CLEC-1 -- CLEC-2 -- CLEC4E -- CLEC5A -- Clk -- Copine -- CREB -- Csk -- Csk homologous kinase -- CXCL10 [Chemokine (C-X-C motif) ligand 10 or IP-10] -- Cyclin A -- Dap kinase -- Darpp -- Dbf4 -- Dect in-1 -- delta glutamate receptor -- DHHC -- DLK (dual leucine zipper-bearing kinase) -- Dock2 -- Drak2 -- Ect2 (epithelial cell transforming 2 oncogene) -- eIF5 -- eIF6 -- Endo180 -- Endothelin A receptor -- Eph receptor -- EphA3 -- Eps8 (epidermal growth factor receptor pathway substrate 8) -- Erk1/Erk2 -- Erk3/Erk4 -- ERK5/MEK5 -- Estrogen receptor -- Fbln -- FGF (fibroblast growth factor) -- Fgr -- Fhit -- Flotillin 1 -- Flotillin 2 -- Fn14 -- Follicle stimulating hormone receptor -- Formyl peptide receptor -- FOXO1 -- FPR2/ALX -- Frabin -- Frs2 -- FZD (frizzled homolog) -- G alpha i-class G protein alpha subunits -- G protein alpha 12 -- G protein alpha q -- G protein alpha transducin -- G protein beta/gamma -- Gab1 -- Gab2 -- GABA A receptor -- Gadd45 -- Galanin receptor -- Gat (GABA transporter) -- GATA-3 (GATA binding protein 3) -- GCAP (guanylyl cyclase activating protein) -- Geft -- Glucose dependent insulinotropic polypeptide receptor -- Glutamate receptors -- Gpr84 -- Grp94 (HSP90B1) -- Gsk (glycogen synthase kinase) -- GST (glutathione S-transferase) -- Guanylate cyclase -- Guanylyl cyclase C -- Guanylyl cyclase receptors -- HB-EGF (heparin-binding EGF-like growth factor) -- Hipk2 -- Hippocalcin -- hnRNP (heterogeneous nuclear ribonucleoprotein) -- Homer -- Hpk1 -- IAP -- Icmt (Isoprenylcysteine carboxyl methyltransferase) -- Id4 (Inhibitor of DNA binding 4) -- IFN gamma -- IGF1R (insulin-like growth factor 1 receptor) -- IL6 -- IL7 -- Inositol 1,4,5-triphosphate-associated cGMP kinase substrate -- Integrin alpha 1 -- Integrin alpha 11 -- Integrin alpha 2 -- Integrin alpha 4 -- Integrin alpha E -- Integrin alpha V -- IP3 receptor -- IRF5 -- IRG (immunity-related GTPase) -- Itpk1 (inositol 1,3,4-triphosphate 5/6 kinase) -- Itsn -- Laforin -- Lat (linker for activation of T cells) -- LCoR -- LIMK -- Lysyl oxidase -- MAIL (IkBz) -- Malt1 (mucosa associated lymphoid tissue lymphoma translocation gene 1) -- MAP kinases (comprising 14 genes: Erk1, Erk2, Erk3, Erk4, Erk5, Erk7, Jnks (3 isoforms), p38 (4 isoforms) and Nlk) -- Mapkap kinase 2/3 (MK2/3) -- Mapkap kinase 5 (MK5)(PRAK) -- Mark (MAP/microtubule affinity-regulating kinase) -- Mek -- Mek3 -- Mk-styx -- MKK6 -- Mlk (mixed lineage kinase) -- MLTK -- MORF/MOZ lysine acetyltranferase -- Mps1 -- Msk1 -- mTOR -- Mtus1 -- Myc -- MyD88 (myeloid differentiation primary response gene 88) -- MYLK (Myosin linght chain kinase) -- Myosin I (Myo1) -- Myosin III -- Myosin X -- N-WASP -- Ncam (neural cell adhesion molecule) -- Nek (never in mitosis gene a-related expressed kinase) -- Net1 (neuroepithelial cell transforming gene 1 protein) -- Neurotensin receptor -- NF-kB family -- NFAT -- Nherf -- NK receptor -- NKG2D -- NKp46 -- NMT (N-myristoyltransferase) -- Notch -- Nrf2 (NF-E2-related factor2) -- nrip1(Nuclear Receptor-Interacting Protein 1) -- NTPDase family -- Nucleotide receptor P2x -- Nucleotide receptor P2y -- Olfactory receptor -- Opioid receptor -- P-Rex -- p130Cas -- P2Y14 receptor -- P38 MAPK -- P53 -- Pak2 -- PCAF lysine acetyltransferase -- Pea15 -- Phlda1 (T-cell death associated gene)(Pleckstrin homology-like domain, family A, member 1) -- Phosphodiesterase 1 -- Phosphodiesterase 10A -- Phosphodiesterase 4, cAMP specific -- Phosphoinositide 3-kinase -- Phosphoinositide 5-phosphate 4-kinase -- Phospholipase A -- Phospholipase D -- Pim-1 -- Pin1 (peptidylprolyl cis/trans isomerase, NIMA-interacting 1) -- Pink1 -- PKD -- PKR -- Plasma membrane calcium transporting ATPase -- Plekhg5 -- Polycystin -- PP2C -- Prep -- Prion protein -- Protein farnesyltransferase -- Psgr (prostate specific G-protein coupled receptor) -- PTEN -- PTK6 -- PTPe (RPTPe and cyt-PTPe) -- PTPN3/PTPN4 -- R7BP/R9AP -- Rab18 -- Rab23 -- Rab 7 -- Rab 8 -- Rac -- Raf1 -- Ramp -- Ran -- RANKL and RANK -- Rap GEF family -- Ras (H-, K-, N-Ras) -- RASA1 -- Rasgrf (RAS protein-specific guanine nucleotide-releasing factor) -- RasGRP1 -- Rcan1 (regulator of calcineurin) -- Recoverin -- Relaxin family peptide receptor: 1 & 2 -- Relaxin family peptide receptor: 3 & 4 -- Retinoic acid receptor -- RGS family -- Rgs13 -- RhoC -- Rhodopsin kinase -- Ric8 -- Rin (Ras-like protein in neuron) -- RIN family proteins (RIN1, RIN2 and RIN3) -- ROCK kinases -- Rpn8 -- Rpt -- Rrad (Ras-related associated with diabetes) -- Rsk (p90 ribosomal S6 kinase) -- RyR (ryanodine receptor) -- S100 Proteins -- SAMSN1 (SAM domain, SH3 domain and nuclear localization signal) -- SARA -- Sh2d2a (SH2 domain protein 2A) -- SHIP -- SHP-1 -- SKAP-HOM -- Slp (synaptotagmin-like protein) -- SLP-76 (SH2 domain containing leukocyte protein of 76kDa) -- SOCS -- Somatostatin receptor -- Sonic hedgehog -- Sphingosine-1-phosphate -- Src -- Sry -- STAT -- Striatal-Enriched Protein Tyrosine Phosphatase (STEP) -- Sulfiredoxin -- SWI/SNF (SWItch/Sucrose NonFermentable) -- TBCCD1 -- Tead -- The Steroid Receptor Coactivator Family -- Thrombospondin 1 -- TLR adaptor protein -- TLR3 -- TLR4 -- TLR5 -- TLR9 -- TMEM85 (transmembrane protein 85) -- TPD52 (tumor protein D52) -- Tpl2 -- TRAF6 -- TRAIL receptor 1/2 (death receptor 4/5, DR4/5) -- Trb (tribbles) -- Trp (transient receptor potential cation channel) -- Urokinase-type plasminogen activator receptor -- UT (Urea transporter) -- VAMP1/2/3/7 -- Vav Family -- Vitamin D receptor -- VRK1 -- VRK2 -- VRK3 -- WASH -- ZAP-70 -- ZNF202 (zinc finger protein 202; Zfp202) -- ZnT (zinc transporter)
    Abstract: Biological processes are driven by complex systems of functionally interacting signaling molecules. Thus, understanding signaling molecules is essential to explain normal or pathological biological phenomena. A large body of clinical and experimental data has been accumulated over these years, albeit in fragmented state. Hence, systems biological approaches concomitant with the understanding of each molecule are ideal to delineate signaling networks/pathways involved in the biologically important processes. The control of these signaling pathways will enrich our healthier life. Currently, there are more than 30,000 genes in human genome. However, not all the proteins encoded by these genes work equally in order to maintain homeostasis. Understanding the important signaling molecules as completely as possible will significantly improve our research-based teaching and scientific capabilities. This encyclopedia presents 350 biologically important signaling molecules and the content is built on the core concepts of their functions along with early findings written by some of the world’s foremost experts. The molecules are described by recognized leaders in each molecule. The interactions of these single molecules in signal transduction networks will also be explored. This encyclopedia marks a new era in overview of current cellular signaling molecules for the specialist and the interested non-specialist alike During past years, there were multiple databases to gather this information briefly and very partially. Amidst the excitement of these findings, one of the great scientific tasks of the coming century is to bring all the useful information into a place. Such an approach is arduous but at the end will infuse the lacunas and considerably be a streamline in the understanding of vibrant signaling networks. Based on this easy-approach, we can build up more complicated biological systems
    Pages: XLVIII, 2030 p. 300 illus., 100 illus. in color. : online resource.
    ISBN: 9781461464389
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  • 25
    Keywords: Oncology ; Immunology ; Human Genetics ; Medicine ; Cytology ; Cancer Research ; Immunology ; Human Genetics ; Molecular Medicine ; Cell Biology ; Medicine/Public Health, general ; Springer eBooks
    Description / Table of Contents: Foreword -- Mast cell ontogeny -- Staining of mast cells -- Mast cells heterogeneity -- Mast cell biology and functions -- Mast cells in arteriogenesis -- Mast cells in primary systemic vasculitides -- Mast cells in blood-brain barrier alterations and neurodegenerative diseases -- Mast cells and basophils: a link between angiogenesis and inflammation in allergic diseases -- Mast cells in tumor fate -- Mast cells in tumor angiogenesis and lymphoangiogenesis -- Mast cells as therapeutic target in cancer -- Index
    Abstract: This book covers the most important aspects of Mast Cell Biology. It enables the reader to immerse in the new aspects of Mast Cells biology, which are no longer seen as an immunological effector cell, but a multi-functional efector cell. One which have roles that expands beyond the immunological realm and shows deep implications in the pathophysiology of several human disorders as well as transforming into a very important and promising target for therapy development in cancer, neurodegerative disorders, angiogenesis, inflammation and allergy. Across twelve chapter on Mast Cells Research, starting from the discovery and characterization of Mast Cells until the lastest findings and translational perspectives, the reader will get a complete view and a profound insight on the fundamental and applied aspects of Mast Cells Biology. The chapters are written in a clear and concise way in order to help navigating through complex biological scenarios. The author, Domenico Ribatti, Full professor of the University of Bari, is an expert in the field of Mast Cells with more than 800 publications
    Pages: VII, 112 p. 12 illus., 8 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030241902
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  • 26
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    Singapore : Springer
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Protective Responses of Different Organs to Environmental Toxicants or Stresses -- Avoidance Behaviour of Nematodes to Environmental Toxicants or Stresses -- Intestinal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Epidermal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Toxicity Induction in Intestine and Epidermis in Nematodes Exposed to Environmental Toxicants or Stresses -- Toxicity Induction in Neurons and Muscle in Nematodes Exposed to Environmental Toxicants or Stresses -- Reproductive Toxicity Induction in Nematodes Exposed to Environmental Toxicants or Stresses -- Intestinal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Epidermal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Neuronal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Germline Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses
    Abstract: This book introduces readers to intestinal and epidermal barriers, and to toxicity induction of environmental toxicants or stresses in the intestine, epidermis, neurons, muscle, and reproductive organs in Caenorhabditis elegans. In addition, it discusses the protective responses of various organs and nematodes’ avoidance behaviour with regard to environmental toxicants or stresses. The intestinal, epidermal, neuronal, and germline signalling pathways required for the regulation of toxicity of environmental toxicants or stresses are also introduced and discussed. As a classic model animal with well-described genetic and developmental backgrounds, the nematode Caenorhabditis elegans has been successfully and widely used in both toxicity assessments and toxicological studies on various environmental toxicants and stresses. Once exposure to certain environmental toxicants has occurred, the toxicants can enter into the primary targeted organs (such as intestinal cells), and even be translocated into secondary targeted organs (such as reproductive organs and neurons). Based on related available data, this book provides a systematic understanding of target organ toxicology in C. elegans
    Pages: XIII, 337 p. 232 illus., 175 illus. in color. : online resource.
    ISBN: 9789811360107
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  • 27
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction -- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction -- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells -- Isolation and Characterization of Cutaneous Epithelial Stem Cells -- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin -- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells -- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis -- Isolation and Cultivation of Epidermal (Stem) Cells -- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen -- Isolation and Cultivation of Skin-Derived Precursors -- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells -- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor -- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair -- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet -- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering -- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration -- Skin Wound Healing: Refractory Wounds and Novel Solutions -- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice -- Melanoblasts as Multipotent Cells in Murine Skin -- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro -- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells -- Isolating Immune Cells from Mouse Embryonic Skin -- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells -- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy -- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications -- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis -- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells -- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research -- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma -- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells -- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
    Abstract: This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells
    Pages: XV, 463 p. 105 illus., 76 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988709
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  • 28
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 29
    Keywords: Bioinformatics ; Cytology ; Bioinformatics ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Quality Control of Single-cell RNA-seq -- Normalization for Single-cell RNA-seq Data Analysis -- Analysis of Technical and Biological Variability in Single-cell RNA Sequencing -- Identification of Cell Types from Single-cell Transcriptomic Data -- Rare Cell Type Detection -- scMCA- A Tool Defines Cell Types in Mouse Based on Single-cell Digital Expression -- Differential Pathway Analysis -- Differential Pathway Analysis -- Estimating Differentiation Potency of Single Cells using Single Cell Entropy (SCENT) -- Inference of Gene Co-expression Networks from Single-Cell RNA-sequencing Data -- Single-cell Allele-specific Gene Expression Analysis -- Using BRIE to Detect and Analyse Splicing Isoforms in scRNA-seq Data -- Preprocessing and Computational Analysis of Single-cell Epigenomic Datasets -- Experimental and Computational Approaches for Single-cell Enhancer Perturbation Assay -- Antigen Receptor Sequence Reconstruction and Clonality Inference from scRNA-seq Data -- A Hidden Markov Random Field Model for Detecting Domain Organizations from Spatial Transcriptomic Data
    Abstract: This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis
    Pages: X, 271 p. 168 illus., 156 illus. in color. : online resource.
    ISBN: 9781493990573
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  • 30
    Keywords: Biochemistry ; Cytology ; Protein Science ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cloning and Mutagenesis Strategies for Large Collagens -- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen -- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds -- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils -- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy -- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry -- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes -- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics -- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy -- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology -- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics -- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix -- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans -- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis -- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment -- FACS Analysis of Col1α Protein Levels in Primary Fibroblasts -- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors
    Abstract: This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study
    Pages: XI, 254 p. 60 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990955
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  • 31
    Keywords: Toxicology ; Embryology ; Cytology ; Pharmacology/Toxicology ; Embryology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vitro Models in Developmental Toxicology -- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments -- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology -- Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model -- Micromass Methods for the Evaluation of Developmental Toxicants -- The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System -- Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology -- Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology -- Hepatotoxicity in Zebrafish Larvae -- Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model -- The Chicken Embryo as a Model in Developmental Toxicology -- The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity -- Mouse Whole Embryo Culture -- Rat Whole Embryo Culture -- Rabbit Whole Embryo Culture -- Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology -- Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish -- Assessment of Histiotrophic Nutrition Using Fluorescent Probes -- Examining Gene Expression Patterns through Whole Mount In Situ Hybridization -- Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos -- Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection -- Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA -- Western Analysis of Breast Cancer 1 Protein (BRCA1) -- Regulation and Control of AP-1 Binding Activity in Embryotoxicity -- Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) -- The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity -- Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining
    Abstract: This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures
    Pages: XI, 436 p. 155 illus., 104 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991822
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  • 32
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    Basel : Springer
    Keywords: Immunology ; Toxicology ; Emerging infectious diseases ; Cytology ; Rheumatology ; Immunology ; Pharmacology/Toxicology ; Infectious Diseases ; Cell Biology ; Rheumatology ; Springer Nature Living Reference
    Description / Table of Contents: Adhesion molecules of neutrophils -- Anaphylaxis (Immediate hypersensitivity): from old to new mechanisms -- Antiviral Responses -- Autophagy and Inflammation -- Basophils -- Cell signalling in neutrophils -- Co-stimulatory Receptors -- Dendritic Cells -- GILZ-Related Regulation of Inflammation -- Glial Cells -- Immunoglobulin receptors and inflammation.-Inflammation and autoimmunity -- Inflammatory Bowel Disease Models in Animals -- Leukocyte Recruitment -- Lymphocyte homing and trafficking -- Macrophage heterogeneity during inflammation -- Mast Cells -- Mechanisms of Macrophage Migration in 3-Dimensional Environments -- Microvascular Responses to Inflammation -- Modulation of inflammation by key nutrials -- Natural Killer Cells -- Neutrophil Extracellular Traps -- Neutrophil oxidative burst -- Obesity and Inflammation -- Osteoclasts in Inflammation -- Parasitism: A Unique Lifestyle Reflecting Dynamic Parasite- and Host(s)-Co-driven Processes -- Platelet, Endothelium, and Inflammation -- Regulatory T cells -- Sepsis Models in Animals -- Skin inflammation models in animals -- Th17 Response -- Toll-Like Receptors -- Angiogenesis Inhibitors -- Anti-asthma Drugs, Overview -- Anti-histamines -- Anti-muscarinics -- Antibiotics as anti-inflammatory drugs -- Antimalarial Drugs -- Azathioprine/Thiopurines -- Beta2 Agonists -- CD 20 Inhibitors – Rituximab -- CD80/86 Inhibitors -- Chromones – Cromoglycate and Nedocromil -- Colony stimulating factor therapeutics -- Corticosteroids -- Coxibs -- Disease-Modifying Anti-Rheumatic Drugs -- Fenamates -- Flavonoids as anti-inflammatory agents -- Gold Complexes -- Interleukin 1 Inhibitors -- Interleukin 12/23 Inhibitors: Ustekinumab Interleukin-6 Inhibitor – Tocilizumab -- Leflunomide -- Medicinal Fatty Acids -- Methotrexate.-NO-donating anti-inflammatory drugs -- Non-steroidal anti-inflammatory drugs -- Phosphodiesterase-4 inhibitors -- Propionic Acid Derivative Drugs (Profens) -- Salicylates -- Sulfasalazine and Related Drugs -- Tetracyclines -- Theophylline -- TNF Inhibitors -- Acute exacerbations of airway inflammation -- Allergic Disorders -- Alzheimer's Disease -- Antiphospholipid antibody syndrome -- Asthma -- Atopic dermatitis -- Autoinflammatory syndromes -- Behcet’s disease -- Bronchiolitis obliterans -- Cancer and inflammation -- Cogan’s syndrome -- COPD -- Diabetes type I -- Diabetes type II -- Diffuse panbronchiolitis -- Gaucher's disease -- Genetic susceptibility to inflammatory diseases -- Gout -- Graft vs Host Disease -- Granulomatosis with Polyangiitis (GPA) -- Grave’s disease.-Guillaine-Barre disease -- Henoch-Schönlein Purpura -- Idiopathic Thrombocytopenic Purpura -- Inflammatory bowel disease -- Juvenile idiopathic arthritis -- Kawasaki Disease -- Lyme disease -- Osteoarthritis.-Osteoarthritis Genetics -- Polymyositis and Dermatomyositis -- Rheumatic Fever -- Rheumatoid arthritis -- Role of Cell Therapy in Autoimmune Disease -- Sarcoidosis -- Sepsis -- Spondylarthropathies -- ACKR3 -- Adhesion molecules -- Alarmins -- Antibacterial Host .-Defense Peptides -- Bacterial Lipopolysaccharide.-Biofilm -- Bone Morphogenetic Proteins in Inflammation -- C5a Receptors -- CCL18 -- CCR1 -- Ceramide 1-Phosphate: A Mediator of Inflammatory Responses -- Complement System -- CXCL4 und CXCL4L1 -- CXCR1 and CXCR2 and Ligands -- CXCR3 and its Ligands -- Cytokines -- Cytolytic Granules -- Foxp3 -- Heat Shock Proteins -- Histamine -- IFNgamma -- IkappaB -- Inflammasomes -- Interleukin 1 -- Interleukin 10 -- Interleukin 12 -- Interleukin 17 -- Interleukin 18 -- Interleukin 18 Binding Protein -- Interleukin 2 -- Interleukin 22 -- Interleukin 23 -- Interleukin 27 -- Interleukin 32 -- Interleukin 36 Cytokines -- Interleukin 4 and the related cytokines (Interleukin 5 and Interleukin 13) -- Interleukin 6 -- Interleukin 9 -- Interleukin-33 -- Janus Kinases (JAKs)/STAT Pathway -- Kinins -- Leukotrienes -- Mammalian Target of Rapamycin (mTOR) -- MAP Kinase Pathways -- NFkappaB -- Nitric oxide -- Nuclear Receptor Signaling in the Control of Inflammation -- Pathogen-Associated Molecular Patterns (PAMPs) -- Pentraxins -- Prostanoids -- Protease-Activated Receptors -- Reactive oxygen species -- Substance P in Inflammation -- TGF-b superfamily cytokine MIC-1/GDF15 in health and inflammatory diseases -- TNFalpha -- Type I Interferons
    Abstract: Inflammation has become one of the most exciting and rewarding areas of medical research. Recent years have seen a revolution in our understanding of how blood and tissue cells interact and of the intracellular mechanisms controlling their activation. This has revealed the underlying inflammatory pathology of many diseases and provided multiple new targets for anti-inflammatory and immunomodulatory therapy. The Encyclopedia of Inflammatory Diseases will cover the following areas: Inflammatory Processes and Cells Inflammatory Diseases Mediators of Inflammation Pharmacology of Inflammation Since inflammatory diseases and their therapy cover a broad range of scientific and medical fields, the encyclopedia will be co-edited by four international experts, a clinician and three researchers from the disciplines of immunology, biochemistry, and pharmacology, in this way providing students, basic and clinical scientists and practitioners in academia, hospitals and industry with valuable interlinked information. This living project will serve as a reliable and comprehensive data pool for everybody working in inflammation research. Owing to its dynamic nature, it will grow with time and future editions, becoming an indispensible source of information for academia, clinical practitioners and industry
    Pages: 1600 p. : online resource.
    ISBN: 9783034806206
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  • 33
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Detecting Cellular Senescence in Reprogramming -- DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA) -- Reactive Oxygen Species Detection in Senescent Cells -- Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo -- Relative Human Telomere Length Quantification by Real-time PCR -- Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP) -- Measuring the Inflammasome in Oncogene-induced Senescence -- Alarmin Detection in Senescent Cells -- IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence -- Genotoxic Stress-induced Senescence -- A Multi-parametric Assay to Evaluate Senescent Cells -- A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids -- Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry -- Quantification of Autophagy during Senescence -- Quantification of Aneuploidy in Mammalian Systems -- Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis -- Mouse Models of Accelerated Cellular Senescence -- Methods to Quantify the NF-kB Pathway during Senescence
    Abstract: This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 253 p. 45 illus., 38 illus. in color. : online resource.
    ISBN: 9781493989317
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  • 34
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: An Update on the Therapeutic Potential of Stem Cell -- Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells -- Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche -- Identification and Isolation of Mice and Human Hematopoietic Stem Cells -- Identification and Characterization of Hair Follicle Stem Cells -- Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier -- 3D Bioprinting and Stem Cells -- Characterization of Gastrospheres Using 3D Coculture System -- Markers and Methods to Study Intestinal Stem Cells -- Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays -- Detection, Labeling, and Culture of Lung Stem and Progenitor Cells -- Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells -- Isolating and Characterizing Adipose-Derived Stem Cells -- Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell -- Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells -- The Maintenance of Tendon Stem/Progenitor Cells in Culture -- Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration -- Beating Heart Cells from Hair Cells -- Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells -- Analytical Platforms and Techniques to Study Stem Cells Metabolism
    Abstract: This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine
    Pages: XI, 284 p. 58 illus., 52 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493986972
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  • 35
    Keywords: Oncology ; Human Physiology ; Cytology ; Cell receptors ; Gene Expression ; Oncology   ; Cancer Research ; Human Physiology ; Cell Biology ; Receptors ; Gene Expression ; Oncology ; Springer eBooks
    Description / Table of Contents: Chapter 1. 1. Overview of tyrosine kinases and their receptors in gastrointestinal malignancies- -- Chapter 2. EGFR and Erb-B2R (HER2): their role in Esophagus cancer -- Chapter 3. HGFR and FGFR2: their role in progression and metastasis of Esophagus cancer -- Chapter 4. Role of Src and VEGF R tyrosine kinases in Esophagus cancer -- Chapter 5. HGF receptor tyrosine kinase in gastric cancer. - Chapter 6. The EGF/ErbB receptor family of tyrosine kinases in gastric cancer -- Chapter 7. Helicobacter pylori CagA protein: its role and interaction tyrosine kinases in gastric cancer -- Chapter 8. RON receptor tyrosine kinase in pancreatic cancer progression -- Chapter 9. EGFR and Cytoplasmic kinases Src targeting in pancreatic cancer -- Chapter 10. VEGF and PDGFR targeting in pancreatic cancer -- Chapter 11. c-KIT, and FLT-3: their role in Hepatocellular carcinoma -- Chapter 12. VEGFR, and PDGFR: their targeting in Liver Cancer -- Chapter 13. EGFR and FGFR in growth and metastasis of colorectal cancer -- Chapter 14. VEGFR, and PDGFR: their targeting in colorectal cancerm -- Chapter 15. Nano materials and their impact on tyrosine kinase on selected gastrointestinal malignancies -- Chapter 16. Tyrosine kinase inhibitors and their clinical prospective in gastrointestinal malignancies -- Chapter 17. Drug resistance against tyrosine kinase inhibitors in gastrointestinal malignancies -- Chapter 18. Tyrosine kinases: their expression, diagnostic, therapeutic and prognostic aspects in gastrointestinal cancers
    Abstract: The aim of this book is to provide comprehensive overview of the role of different tyrosine kinases in the progression and metastasis of various cancers of the gastrointestinal tract such as esophagus, liver, pancreas, stomach, and colorectal. Activation of various signaling pathways and tyrosine kinases are responsible for resistance to chemo and radiotherapy in these gastrointestinal malignancies. Targeting these tyrosine kinases, which regulate the activities of survival pathways in growth, and metastasis is a rational strategy in gastrointestinal cancer therapy. Each chapter embedded in this book covers information pertaining to a specific tyrosine kinase and its impact on various malignancies, which is not only significant for basic and clinical research investigations but will also be valued by students at advanced undergraduate to postgraduate levels
    Pages: XV, 241 p. 28 illus., 21 illus. in color. : online resource.
    ISBN: 9789811314865
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  • 36
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots -- Detection and Quantification of the Bicoid Concentration Gradient in Drosophila Embryos -- Imaging Cytonemes in Drosophila Embryos -- Measuring Dorsoventral Pattern and Morphogen Signaling Profiles in the Growing Neural Tube -- Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish -- FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos -- Generation of Ectopic Morphogen Gradients in the Zebrafish Blastula -- Polarizing Region Tissue Grafting in the Chick Embryo Limb Bud -- Analysis of PLETHORA Gradient Formation by Secreted Peptides during Root Development -- Live Imaging of mRNA Transcription in Drosophila Embryos -- LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos -- Discrete-State Stochastic Modeling of Morphogen Gradient Formation -- Simulation of Morphogen and Tissue Dynamics -- Mechanisms and Measurements of Scale Invariance of Morphogen Gradients -- Scaling of Morphogenetic Patterns -- Modeling Time-Dependent Acquisition of Positional Information
    Abstract: This detailed book presents methods focusing on the visualization of morphogen gradients, the analysis of their biophysical and biological properties, and the theoretical aspects underlying their functions. The study of morphogen gradients combines biophysics, cell and developmental biology, and applied mathematics approaches to understand their formation, their biological functions, and the prediction of their behavior in space and time, and this volume serves as a practical, hands-on guide to the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and helpful, Morphogen Gradients: Methods and Protocols is an ideal collection for researchers in this vital area of study
    Pages: XI, 306 p. 143 illus., 136 illus. in color. : online resource.
    ISBN: 9781493987726
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  • 37
    Keywords: Oncology ; Human Genetics ; Medicine ; Cytology ; Drug Interactions ; Cancer Research ; Human Genetics ; Molecular Medicine ; Cell Biology ; Drug Resistance ; Springer eBooks
    Description / Table of Contents: Preface -- Foreword. Understanding and targeting minimal residual disease: a path to change cancer patient outcomes -- Epigenetics and Pluripotency Aspects of Disseminated Cancer Cells during Minimal Residual Disease -- Anti-angiogenic therapy-mediated endothelial damage: A driver of breast cancer recurrence?- Minimal Residual Disease in Prostate Cancer -- Minimal Residual Disease in Head and Neck Cancer and Esophageal Cancer -- Detection of Minimal Residual Disease and its Clinical Applications in Melanoma and Breast Cancer Patients Selena -- Preservation of Quiescent Chronic Myelogenous Leukemia Stem Cells by the Bone Marrow Microenvironment.-Minimal Residual Disease in Acute Myeloid leukemia -- Characteristics and therapeutic targeting of minimal residual disease in childhood acute lymphoblastic leukemia -- Minimal Residual Disease in Multiple Myeloma: Impact on response assessment, prognosis and tumor heterogeneity -- Index
    Abstract: This book focuses on the biological mechanisms of minimal residual disease (MRD) and recurrence. It integrates this biology in solid cancers and in hematological malignancies. It reports also on technological advancements for monitoring MRD, derived from mechanistic insights. Chapters in solid and hematological malignancies address stem cell biology, genetics, epigenetics and micro-environmental regulation of dormant MRD. Novel insight into technologies for molecular phenotyping of MRD and monitoring of CTCs, DTCs and cell free RNA and DNA are also addressed extensively. Five chapters explore the above concepts in solid cancers such as prostate, breast, melanoma, head and neck and esophageal. Two chapters also explore the basic mechanisms of vascular biology targeting and epigenetic mechanisms regulating pluripotency programs during dormancy. Similar biology is explored in hematological malignancies such as T-ALL, CML, AML and multiple myeloma in additional four chapters. This book is edited and prefaced by Dr. Julio Aguirre-Ghiso, an expert in dormancy and recurrence. The chapters are written by world-recognized experts Drs. Ravi Bahtia, Samir Parekh, Russel Taichman, Monica Guzman, David Hoon, Denis Schewe, Irmela Jeremias, Cyrus Ghajar, Maria Soledad Sosa and Nicholas Stoecklein. The topic of this book is of particular interest to both basic cancer cell biologists and physician scientists that are working to provide a more integrated view of the biology of MRD and to those interested in working on or learning about this paradigm. The integrated and cross-disciplinary focus of the book from biology to medicine seeks to help bridge gaps to improve cancer care and prevent recurrences
    Pages: IX, 162 p. 13 illus., 10 illus. in color. : online resource.
    ISBN: 9783319977461
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  • 38
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Immobilisation of Non-activated Unfixed Platelets for Real Time Single-cell Analysis -- Imaging Platelets and Megakaryocytes by High Resolution Laser Fluorescence Microscopy -- Single Molecule Localisation and Structured Illumination Microscopy of Platelet Proteins -- Electron Tomography and Correlative Approaches in Platelet Studies -- Screening and High-throughput Platelets Assays -- High-throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry -- Precise Quantification of Platelet Proteins and their Phosphorylation States -- The Study of Platelet Receptors using Artificial Lipid Bilayers -- Three Dimensional Culture in a Methylcellulose-based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation -- Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor Driven Forward Programming -- Three-dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production -- Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes -- High-resolution 3D Imaging of Megakaryocytes using Focused Ion Beam-Scanning Electron Microscopy -- Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-sheet Fluorescence Microscopy -- Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches
    Abstract: The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls. Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study
    Pages: XI, 290 p. 72 illus., 52 illus. in color. : online resource.
    ISBN: 9781493985852
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  • 39
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: EM Tomography of LINC Complexes -- Recombinant Purification of the Periplasmic Portion of the LINC Complex -- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis -- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin -- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID -- Using Nesprin Tension Sensors to Measure Force on the LINC Complex -- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei -- Direct Force Probe for Nuclear Mechanics -- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning -- Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments -- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy -- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging -- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans -- High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development -- Computational Methods for Studying the Plant Nucleus -- Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System -- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components -- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice -- An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle -- Functional Analysis of LINC Complexes in Skin -- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer -- Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
    Abstract: This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIV, 338 p. 61 illus., 41 illus. in color. : online resource.
    ISBN: 9781493986910
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  • 40
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Short Overview of PINK1/Parkin-mediated Mitophagy -- Induction of PINK1/Parkin-Mediated Mitophagy -- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells -- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy -- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila -- Assessment of Mitophagy in iPS Cell-derived Neurons -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting -- Mitopho8∆60 Assay as a Tool to Quantitatively Measure Mitophagy Activity -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants -- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy -- Exploring Micrornas on Nix-Dependent Mitophagy -- Monitoring of Atg5-Independent Mitophagy -- Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans -- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells -- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans -- Iron-Starvation Induced Mitophagy in Yeast
    Abstract: This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson’s disease, especially young onset Parkinson’s disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy
    Pages: XI, 180 p. : online resource.
    ISBN: 9781493977505
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  • 41
    Keywords: Human Genetics ; Biochemistry ; Cytology ; Human Genetics ; Biochemistry, general ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Chapter 1 An overview of circular RNAs -- Chapter 2 RNA-sequencing and the predictions tools for circular RNAs -- Chapter 3 Online databases and circular RNAs -- Chapter 4 Circular RNA splicing -- Chapter 5 Circular RNAs biogenesis in eukaryotes through self-cleaving hammerhead ribozymes -- Chapter 6 Circular RNAs act as miRNA sponges -- Chapter 7 Regulation of transcription by Circular RNAs -- Chapter 8 Functional Analysis of Circular RNAs -- Chapter 9 Circular RNA in Exosomes -- Chapter 10 Circular RNAs in Blood -- Chapter 11 Circular RNA in Saliva -- Chapter 12 Emerging role of circular RNAs as potential biomarkers for the diagnosis of Human Diseases -- Chapter 13 Circular RNAs as novel biomarkers for cardiovascular diseases -- Chapter 14 Circular RNAs as biomarkers for cancer -- Chapter 15 Circular RNAs in cardiovascular diseases -- Chapter 16 Circular RNAs and Neuronal Development -- Chapter 17 Circular RNAs in Cancer -- Chapter 18 Circular RNAs in Brain Physiology and Disease -- Chapter 19 Circular RNA and Alzheimer’s Disease -- Chapter 20 Circular RNA in Liver: Health and Diseases -- Chapter 21 Circular RNAs in Organ Fibrosis -- Chapter 22 Circular RNAs in Metabolic Diseases -- Chapter 23 Circular RNAs in vascular functions and diseases -- Chapter 24 Functional role of circular RNA in regenerative medicine -- Chapter 25 The role of circular RNAs in cerebral ischemic diseases: ischemic stroke and cerebral ischemia/reperfusion injury -- Chapter 26 CircRNAs in plants -- Chapter 27 Circular RNAs and plant stress responses -- Chapter 28 Prospective advances in circular RNA investigation
    Abstract: This book provides an essential overview of the rapidly advancing field of circular RNAs – newly discovered RNAs that are generated by back-splicing precursor mRNA and perform regulatory functions in many biological processes. Although many aspects of circular RNAs’ biology and mechanisms of gene regulation remain unclear, they have been found to be abundant, evolutionally conserved, and stable in cells; further, they have numerous potential functions. The book consists of eight parts:1) An overview of circular RNAs, 2) Bioinformatics for circular RNAs, 3) Biogenesis of circular RNAs, 4) Molecular mechanisms and gene regulation of circular RNAs, 5) Circular RNAs as potential disease biomarkers, 6) Circular RNAs and human diseases, 7) Circular RNAs in Plants and in Archaea, and 8) Future prospects. Given its focus, the book will be especially useful for researchers and students in the fields of biochemistry, molecular biology, cell biology, and medicine
    Pages: VII, 370 p. 41 illus., 35 illus. in color. : online resource.
    ISBN: 9789811314261
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  • 42
    Keywords: Cytology ; Cardiology ; Biomedical Engineering ; Heart / Surgery ; Cell Biology ; Cardiology ; Biomedical Engineering/Biotechnology ; Cardiac Surgery ; Springer eBooks
    Description / Table of Contents: Preface -- Biomechanical Properties and Mechanobiology of Cardiac ECM -- Imaging the Cardiac Extracellular Matrix -- Animal Models and Cardiac Extracellular Matrix Research -- Applications of Cardiac Extracellular Ma-trix in Tissue Engineering and Regenera-tive Medicine -- Whole Cardiac Tissue Bioscaffolds -- Natural Sources of Extracellular Matrix for Cardiac Repair -- Cardiac Extracellular Matrix Modification as a Therapeutic Approach -- Extracellular Matrix for Myocardial Repair -- Role of Extracellular Matrix in Cardiac Cellular Therapies -- Regulation of Regenerative Medicine Products -- Clinical Trial Design for Investigational Cardio-Regenerative Therapy -- Regenerative Medicine Venturing at the University-Industry Boundary: Implications for Institutions, Entrepreneurs, and Industry -- Index
    Abstract: This book on cardiac extracellular matrix (ECM) features three sections, Fundamental Science, Pre-Clinical and Translational Science, and Clinical Applications. In the Fundamental Science section, we will cover the spectrum of basic ECM science from ECM’s role in development, biomechanical properties, cardiac ECM influence of cardiomyocyte biology, pathophysiology of ECM in heart disease, and ECM in tissue engineering. Section two, Preclinical and Translational Science, will discuss cardiac ECM technologies in the clinical pipeline including approaches to ECM as a therapeutic, animal models of cardiac research, tracking and imaging methods of cardiac ECM, and cGMP manufacturing and regulatory considerations for ECM based therapeutics. Finally, the third section, Clinical Applications, will highlight the clinical experience around cardiac ECM including therapeutic strategies targeting scar tissue in the heart, Clinical trial design and regulatory considerations, current human clinical trials in cardiovascular medicine and the role of pharmaceutical and biotech companies in the commercialization of ECM technologies for cardiovascular indications. This book provides a comprehensive review for basic and translational researchers as well as clinical practitioners and those involved in commercialization, regulatory and entrepreneurial activities
    Pages: XII, 246 p. 35 illus., 33 illus. in color. : online resource.
    ISBN: 9783319974217
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  • 43
    Keywords: Nucleic Acids ; Cytology ; Nucleic Acid Chemistry ; Mathematical and Computational Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction -- Topological structure and biological function of gene network regulated by microRNA -- MicroRNA function of some life process in the gene network -- Controlling the complex biological phenomena using mathematical tools
    Abstract: This book discusses topics related to the topological structure and biological function of gene networks regulated by microRNAs. It focuses on analyzing the relation between topological structure and biological function, applying these theoretical results to gene networks involving microRNA, illustrating their biological mechanisms, and identifying the roles of microRNA in controlling various phenomena emerging from the networks. In addition, the book explains how to control the complex biological phenomena using mathematical tools and offers a new perspective on studying microRNA. It is a useful resource for graduate students and researchers who are working on or interested in microRNAs and gene network
    Pages: XI, 224 p. 99 illus., 58 illus. in color. : online resource.
    ISBN: 9789402415773
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  • 44
    Keywords: Proteomics ; Cytology ; Biochemistry ; Human Genetics ; Cell Physiology ; Proteomics ; Cell Biology ; Animal Biochemistry ; Plant Biochemistry ; Human Genetics ; Cell Physiology ; Springer eBooks
    Description / Table of Contents: The proteome of plant peroxisomes -- Defining the mammalian peroxisomal proteome -- Fungal peroxisomes proteomics -- The craft of peroxisome purification – a technical survey through the decades -- Prediction of peroxisomal matrix proteins in plants -- The obvious and the hidden: Prediction and function of fungal peroxisomal matrix proteins -- Predicting peroxisomal targeting signals to elucidate the peroxisomal proteome of mammals -- Multiple localization by functional translational readthrough -- Evolution of the peroxisomal proteome -- Peroxisome protein prediction in Drosophila melanogaster -- Using Pull Down strategies to analyze the interactome of peroxisomal membrane proteins in human cells -- Identification of peroxisomal protein complexes in mammalian cells -- Unraveling of the structure and function of peroxisomal protein import machineries -- The proteome of fruit peroxisomes: Sweet pepper (Capsicum annuum L.) as a model -- Peroxisomes and their central role in metabolic interaction networks in humans -- Characterisation of peroxisomal regulation networks -- Multi-localized proteins: the peroxisome-mitochondria connection Peroxisome-mitochondria inter-relations in plants -- Peroxisomes and cellular oxidant/antioxidant balance: protein redox -- Cell death or survival against oxidative stress -- A role for RNS in the communication of plant peroxisomes with other
    Abstract: This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, andcontains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del Río is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estación Experimental del Zaidín, Granada, Spain. Del Río’s research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments
    Pages: XIII, 493 p. 55 illus., 49 illus. in color. : online resource.
    ISBN: 9789811322334
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  • 45
    Keywords: Biochemistry ; Cytology ; Neurosciences ; Biochemistry, general ; Biological and Medical Physics, Biophysics ; Cell Biology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Chapter 1.A look at functions that emerge from small numbers -- Chapter 2.Neuronal synaptic connections organized by small numbers of molecules -- Chapter 3.Invasions of small numbers: how many virus particles does it take to infect someone with the flu? -- Chapter 4.Rebellion by the minority: prophecies by molecules on paper and computers -- Chapter 5.The personality of small numbers: do molecules have personality? -- Chapter 6.Distinguishing and searching for minority cells: small in number, but large in effect -- Chapter 7.Digital bioanalysis -- Chapter 8.How small numbers of long genomic DNA are stored in cells -- Chapter 9.Formation by small numbers: minority biological scenarios in correlations among the structure, dynamics, and function of nuclear chromosomes -- Chapter 10.Dividing small numbers: the discreteness and distribution of molecules in the cell membrane -- Chapter 11.Getting to know the functions of small numbers -- Chapter 12.Working in small numbers: The behaviors that emerge when small numbers of bionanomachines team up -- Chapter 13.Thinking small numbers: when, where, and how many molecules there are in the cell -- Chapter 14.Determining small numbers: how the number of flagella is determined -- Chapter 15.Controlling production with small numbers: precision apparatuses made of proteins at work in bacteria -- Chapter 16.Organisms that function with small numbers of molecules -- Chapter 17.How low can you go? The numbers of cells that make up bodies: large numbers and small numbers -- Chapter 18.The flow of time inside the cell: the time of days given by molecules driving the circadian clocks
    Abstract: This book provides an accessible introduction to an exciting new field of life science in which the focus is on small numbers of molecules and minorities within cell populations and their significance for the understanding of biological phenomena. Numbers, or quantitative data, are attracting more attention in cell biology following, for example, determination of the absolute copy number of each protein species in each bacterial cell and the recognition of leader cells that drive collective cell migration. Within this context, the authors present recent advances in experimental techniques, biological findings, and theories. A variety of cutting-edge topics and issues are addressed, with explanation of the ways in which recent developments in the field cast light on seemingly straightforward but difficult-to-answer questions. Readers will learn that we are on the verge of a paradigm shift as the importance of cooperation among groups of molecules in live cells is acknowledged. The book is designed to be enjoyable to read and easy to understand. It will be of interest for a wide range of readers, including young researchers and undergraduate/high school students
    Pages: XII, 150 p. 48 illus., 1 illus. in color. : online resource.
    ISBN: 9789811320835
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  • 46
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Leukocyte-Stromal Interactions within Lymph Nodes -- Stromal Cell Responses in Infection -- Fibroblasts and Osteoblasts in Inflammation and Bone Damage -- Molecular and Cellular Requirements for Tertiary Lymphoid Organs -- Molecular and Cellular Requirements for Tertiary Lymphoid Organs -- Mesenchymal Stem Cells as Endogenous Regulators of Inflammation -- Stromal cells in the Tumor Microenvironment II -- Immunosuppression by Intestinal Stromal Cells -- Novel models to Study Stromal Cell Leukocyte Interactions in Health and Disease
    Abstract: Research into and interest in the role of stromal cells in immunology has exploded over the past 15 years. The conventional view that placed non-hematopoietic stromal cells as passive, structural, and supportive entities has now been replaced with an appreciation that these cells have active, dynamic roles during immune responses, and thus impact on the pathophysiology of multiple immune-mediated diseases. This book serves to provide solid grounding in the fundamentals of stromal immunology, focusing on the biological aspects of their function in addition to highlighting key areas for the development of the field in the future. The book is also a unique source of information on emerging concepts that place stromal cells from outside lymphoid organs as major contributors to the biology of diverse conditions, such as rheumatoid arthritis, chronic parasitic infection, inflammatory bowel disease, and cancer
    Pages: XIII, 151 p. 15 illus. in color. : online resource.
    ISBN: 9783319781273
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  • 47
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Visualization and Measurement of Multiple Components of the Autophagy Flux -- Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells -- Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells -- Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement -- Autophagy in Adipocyte Differentiation -- Assessing Autophagy in the Leydig Cells -- Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells -- Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells -- Autophagy in Zebrafish Extraocular Muscle Regeneration -- The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry -- Cloning of Autophagy-Related MicroRNAs -- Assays to Monitor Aggrephagy in Drosophila Brain -- Methods for Monitoring Autophagy in Silkworm Organs -- Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia -- Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening -- Porcine Cell-Free System to Study Mammalian Sperm Mitophagy -- Monitoring and Measuring Mammalian Autophagy
    Abstract: This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action
    Pages: XII, 228 p. 40 illus., 38 illus. in color. : online resource.
    ISBN: 9781493987481
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  • 48
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Three-Dimensional Visualization of the Lymphatic Vasculature -- The Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels -- Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice -- Visualization and Tools for Analysis of Zebrafish Lymphatic Development -- Characterization of Zebrafish Facial Lymphatics -- Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development -- Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function -- Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice -- Morphological and Functional Analysis of CNS Associated Lymphatics -- Morphological Analysis of Schlemm's Canal in Mice -- Imaging Lymphatics in Mouse Lungs -- Imaging of Endothelial Cell Dynamic Behavior in Zebrafish -- Visualization and Measurement of Lymphatic Function In Vivo -- Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells -- Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo -- Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior -- Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis -- Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro -- Stimulation and Inhibition of Lymphangiogenesis via Adeno-Associated Viral Gene Delivery -- Isolation and Characterization of Mouse Organ Specific Endothelial Transcriptomes -- Single Cell mRNA Sequencing of the Mouse Brain Vasculature -- Metabolic Analysis of Lymphatic Endothelial Cells -- Characterizing Epigenetic Changes in Endothelial Cells
    Abstract: This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field
    Pages: XII, 345 p. 93 illus., 81 illus. in color. : online resource.
    ISBN: 9781493987122
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  • 49
    Keywords: Microbiology ; Animal Physiology ; Cytology ; Biochemistry ; Cell Physiology ; Microbial ecology ; Microbiology ; Animal Physiology ; Cell Biology ; Biochemistry, general ; Cell Physiology ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: Free-Living Protozoa with Endosymbiotic Methanogens -- Anaerobic Ciliates and Their Methanogenic Endosymbionts -- Symbiotic Methanogens and Rumen Ciliates -- Methanogenic and Bacterial Endosymbionts of Free-Living Anaerobic Ciliates -- Termite Gut Flagellates and Their Methanogenic and Eubacterial Symbionts -- Methanogens in the Digestive Tract of Termites -- Methanogenic Archaea in Humans and Other Vertebrates: An Update -- Methanogens in the Gastrointestinal Tract of Animals -- Syntrophy in Methanogenic Degradation -- Hydrogenosomes -- Evolution of Prokaryote-Animal Endosymbiosis from a Genomics Perspective
    Abstract: This updated monograph deals with methanogenic endosymbionts of anaerobic protists, in particular ciliates and termite flagellates, and with methanogens in the gastrointestinal tracts of vertebrates and arthropods. Further chapters discuss the genomic consequences of living together in symbiotic associations, the role of methanogens in syntrophic degradation, and the function and evolution of hydrogenosomes, hydrogen-producing organelles of certain anaerobic protists. Methanogens are prokaryotic microorganisms that produce methane as an end-product of a complex biochemical pathway. They are strictly anaerobic archaea and occupy a wide variety of anoxic environments. Methanogens also thrive in the cytoplasm of anaerobic unicellular eukaryotes and in the gastrointestinal tracts of animals and humans. The symbiotic methanogens in the gastrointestinal tracts of ruminants and other “methanogenic” mammals contribute significantly to the global methane budget; especially the rumen hosts an impressive diversity of methanogens. This makes this updated volume an interesting read for scientists and students in Microbiology and Physiology
    Pages: X, 261 p. 61 illus., 9 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9783319988368
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  • 50
    Keywords: Human Genetics ; Medicine ; Cytology ; Oncology ; Biochemistry ; Human Genetics ; Molecular Medicine ; Cell Biology ; Cancer Research ; Protein Science ; Protein Structure ; Springer eBooks
    Description / Table of Contents: Preface -- Structure of the molecular chaperone prefoldin -- Structure and function of archaeal prefoldin -- Role and functions of prefoldins -- Nuclear functions of prefoldins -- The yeast prefoldin Bud27 -- URI-like prefoldin complex -- HSP90 and its R2TP/Prefoldin-like cochaperone -- Role and functions of URI: mouse models for URI -- Nuclear function of URI-like prefoldin complex -- URI and androgen regulation. Index
    Abstract: This book reviews recent work on molecular chaperones of the prefoldin family from their discovery in Archaea to the basic clarification of their structure. Even though, there is still a lot to investigate in order to understand the roles and functions of prefoldins, some preliminary work suggests their contribution in assembly and activation of a variety of macromolecular complexes. Hence, the reader is introduced to recent structural findings via cryo-electron microscopy and to the importance of this particular group of proteins in health and disease. This book also discusses the particular structure of the URI prefoldin-like complex. In addition, recent developments in the generation of URI mouse models speculate on the functions and emerging roles of the URI prefoldin-like complex in various cellular processes. This novel protein complex represents a unique cellular machinery, which molecular basis of its activity still remains to be determined. This work is thus a guide to help researchers in the field to have a more comprehensive and critical view of the prefoldin world
    Pages: IX, 135 p. 39 illus., 36 illus. in color. : online resource.
    ISBN: 9783030007379
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  • 51
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Tools in the Art of Studying Necroptosis -- Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules -- Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis -- Distinguishing Necroptosis from Apoptosis -- Methods for Studying TNF-Mediated Necroptosis in Cultured Cells -- Analysis of Necroptosis in Bone Marrow-Derived Macrophages -- Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis -- Detection of MLKL Oligomerization during Programmed Necrosis -- Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation -- Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis -- Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis -- Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock -- Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury -- Assessment of In Vivo Kidney Cell Death: Glomerular Injury -- Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling -- Characterization of the TNFR1-SC using ‘Modified Tandem Affinity Purification’ in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS) -- Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex -- Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages
    Abstract: This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field