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  • COMPLEX  (1)
  • ENVIRONMENTAL-CHANGES  (1)
Keywords
  • 1
    Keywords: COMBINATION ; Germany ; GENOME ; microarray ; COMPLEX ; COMPLEXES ; DNA ; SEQUENCE ; SEQUENCES ; antibodies ; antibody ; ARRANGEMENT ; ASSAY ; DNA microarray ; DNA microarray technology ; microarrays
    Abstract: While the deciphering of basic sequence information on a genomic scale is yielding complete genomic sequences in ever-shorter intervals, experimental procedures for elucidating the cellular effects and consequences of the DNA-encoded information become critical for further analyses. In recent years, DNA microarray technology has emerged as a prime candidate for the performance of many such functional assays. Technically, array technology has come a long way since its conception some 15 years ago, initially designed as a means for large-scale mapping and sequencing. The basic arrangement, however, could be adapted readily to serve eventually as an analytical tool in a large variety of applications. On their own or in combination with other methods, microarrays open up many new avenues of functional analysis. Copyright (C) 2003 John Wiley Sons, Ltd
    Type of Publication: Journal article published
    PubMed ID: 18629015
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  • 2
    Keywords: ENERGIES ; CELLS ; EXPRESSION ; GROWTH ; CELL ; EXPOSURE ; SITE ; SITES ; GENE ; GENES ; PROTEIN ; PROTEINS ; SACCHAROMYCES-CEREVISIAE ; transcription ; METABOLISM ; RESPONSES ; DNA ; MECHANISM ; FAMILY ; INDUCTION ; mechanisms ; BINDING ; YEAST ; PROMOTER ; ARRAYS ; cold shock ; DECREASE ; ELEMENTS ; ENERGY ; ENVIRONMENTAL-CHANGES ; heat shock ; HEAT-SHOCK ; MEMBRANE ; NUMBER ; pre-adaptation ; PROMOTERS ; STRESS ; stress response ; STRESS-RESPONSE ; transcriptional profiling
    Abstract: DNA arrays were used to measure changes in transcript levels as yeast cells responded to temperature shocks. The number of genes upregulated by temperature shifts from 30degreesC to 37degreesC or 45degreesC was correlated with the severity of the stress. Pre-adaptation of cells, by growth at 37 degreesC previous to the 45 degreesC shift, caused a decrease in the number of genes related to this response. Heat shock also caused downregulation of a set of genes related to metabolism, cell growth and division, transcription, ribosomal proteins, protein synthesis and destination. Probably all of these responses combine to slow down cell growth and division during heat shock, thus saving energy for cell rescue. The presence of putative binding sites for Xbp1p in the promoters of these genes suggests a hypothetical role for this transcriptional repressor, although other mechanisms may be considered. The response to cold shock (4degreesC) affected a small number of genes, but the vast majority of those genes induced by exposure to 4 degreesC were also induced during heat shock; these genes share in their promoters cisregulatory elements previously related to other stress responses. Copyright (C) 2003 John Wiley Sons, Ltd
    Type of Publication: Journal article published
    PubMed ID: 18629074
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