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  • 1
    ISSN: 1573-0832
    Keywords: DNA ; mitochondria ; restriction profiles ; Sporothrix schenckii ; sporotrichosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract TwoSporothrix schenckii strains of different mitochondrial DNA restriction profiles were isolated from different cutaneous lesions in a 53 year-old woman with sporotrichosis. These results suggest that sporotrichosis can be simultaneously caused by two or more genetically differentS. schenckii strains.
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  • 2
    ISSN: 1573-0832
    Keywords: Sporothrix schenckii ; membrane transporter ; cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have isolated two DNA fragments from Sporothrix schenckii. These fragments were nonspecifically amplified from the whole-cell DNA using polymerase chain reaction (PCR) primers originally designed in the gene encoding heat shock protein 70 of Mycobacterium leprae. Reverse transcription PCR demonstrated that the smaller (sp-2) fragment was expressed, and a database search indicated that the deduced amino acids sequence from the sp-2 fragment contained a region homologous to the conserved sequence of the membrane transporter protein family. This is the first report of partial cloning of the gene encoding the putative membrane transporter in S. schenckii.
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  • 3
    ISSN: 1573-0832
    Keywords: ultrastructure ; Sporothrix schenckii ; iodine ; potassium iodide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ultrastructural changes produced by iodine-potassium iodide solution on yeast cells of Sporothrix schenckii were investigated by transmission electron microscopy in order to clarify the mechanism of oral potassium iodide therapy for sporotrichosis. Yeast cells were dipped with solutions containing various concentrations of iodine. The rate of germination decreased markedly between the range of iodine concentrations from 0.63 μg/ml to 5.0 μg/ml. No significant ultrastructural changes were seen at the concentration of the iodine of 1.25 μg/ml (80% germination) or less. In the concentration of 2.5 μg/ml (50% germination), normal cells and degenerated cells coexisted. When the cells were treated with 5.0 μg of iodine per ml (0% germination) or more, their interior structures were completely destroyed. It is assumed that iodine treatment of the organism causes rapid destruction in the whole cell.
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  • 4
    ISSN: 1573-0832
    Keywords: Sporotrichosis ; Sporothrix schenckii ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The isolation of Sporothrix schenckii from a female European cat it is described. The cat showed lengthened alopecic areas, with prominent nodules in the external surface of the thighs and abdomen. A mycological and histopathological studies of the lesions were carried out. The lesions resolved under treatment with 20% potassium iodide in doses of 0'1 ml/kg oral route in a 8 weeks period.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 111 (1990), S. 75-83 
    ISSN: 1573-0832
    Keywords: Aleurisma carnis ; Amylomyces rouxii ; Aureobasidium pullulans ; Chrysosporium thermophilum ; Corynascus heterothallicus ; Dactylaria gallopava ; Diplorhinotrichum gallopavum ; Geomyces pannorum ; Hansenula ; Mucor rouxii ; Myceliophthora thermophila ; name changes ; Ochroconis gallopava ; Phoma herbarum ; Phoma pigmentivora ; Phoma violacea ; Pichia ; Pullularia pullulans ; Rhizopus arrhizus ; Rhizopus oryzae ; Sporendonema epizoum ; Sporendonema sebi ; Sporothrix schenckii ; Sporotrichum carnis ; Sporotrichum schenckii ; Sporotrichum thermophilum ; taxonomy ; Wallemia sebi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This is the fourth in a series of reports bringing changes in the names of fungi of microbiological, industrial and medical importance to the attention of workers in these fields. The series is sponsored by the Division of Mycology of the International Union of Microbiological Societies (IUMS) and prepared under the auspices of the International Commission on the Taxonomy of Fungi (ICTF). The first of this series of reports included an introduction to the series explaining the need for name changes [1].
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  • 6
    ISSN: 1573-0832
    Keywords: Cellular immunity ; sporotrichosis ; Sporothrix schenckii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sporothrix schenckii is the etiologic agent of sporotrichosis, a mycosis of world-wide distribution more commonly occurring in tropical regions. The immunological mechanisms involved in the prevention and control of sporotrichosis are not fully understood but apparently include both the humoral and cellular responses. In the present investigation, cellular immunity was evaluated by in vivo and in vitro tests in mice infected with yeast-like forms of S. schenckii. The disease developed systemically and cellular immunity was evaluated for a period of 10 weeks. The soluble antigen utilized in the tests was prepared from yeast form of the fungus through the sonication (20 min: 10 sonications at 50 W at 2-min intervals). Delayed hypersensitivity and lymphocyte transformation tests showed that the cellular immune response was depressed between the 4th and 6th week of infection when the animals were challenged with the soluble fungal antigen. This depression frequently indicates worsening of the disease, with greater involvement of the host. This is a promising field of research for a better understanding of the pathogeny of this mycosis.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 116 (1991), S. 165-168 
    ISSN: 1573-0832
    Keywords: Sporothrix schenckii ; sporotrichosis ; pyoderma gangrenosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A 56-year-old female with an eight-year history of corticosteroid therapy for rheumatoid arthritis presented with large, deep, painful ulcers on the left buttock and thigh. The lesions appeared typical of pyoderma gangrenosum. Nine separate cultures of the exudate grew Sporothrix schenckii. During the course of iodide therapy, the patient expired due to Escherichia coli pneumonia. This is the third case report of sporotrichosis presenting as pyoderma gangrenosum and the first report from China. Sporotrichosis presenting as pyoderma gangrenosum is a special form of this disease. It develops quickly and must be treated promptly. Only two cases have been reported in the world literature. This is the first case reported from China.
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  • 8
    ISSN: 1573-0832
    Keywords: Experimental pathogenicity ; Preservation in water ; Sporothrix schenckii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Five strains ofSporothrix schenckii preserved in sterile destillated water were found morphologically stable, viable and pure after a period ranging from 16 to 13 years, in the mycology section culture collection from the Instituto de Medicina Tropical, Universidad Central de Venezuela. Each strain was inoculated into the testes of 3 hamsters. All the animals developed disseminated sporotrichosis.
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  • 9
    ISSN: 1573-0832
    Keywords: Cytokines ; Interleukins ; Interleukin-1 ; Monocytes ; Sporotrichosis ; Sporothrix schenckii ; Tumor necrosis factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Production of Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) by adherent peritoneal cells from BALB/c mice was measured at week 2, 4, 6, 8 and 10 after intravenous inoculation with 106 Sporothrix schenckii yeasts. As compared with age-matched controls, IL-1 and TNF production by adherent peritoneal cells fromS. schenckii-infected mice was reduced severely at week 4 and 6 of infection and greater than normal at week 8 and 10. Moreover, between week 4 and 6 of infection there was a depression of delayed type hypersensitivity response to a specific whole soluble antigen, and an increase in fungal multiplication in the livers and spleens of infected mice. Thus, the deficits of cell-mediated immunity in mice with systemicS. schenckii infection may derive, in part, from impaired amplification of the immune response consequent to abnormal generation of IL-1 and TNF.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 102 (1988), S. 129-133 
    ISSN: 1573-0832
    Keywords: asteroid body ; Sporothrix schenckii ; sporotrichosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A survey of 150 cases with sporotrichosis seen at the Dermatological Clinic of Kurume University Hospital from February 1962 to October 1986 was reported. The proportion of cases with sporotrichosis to the total number of outpatients was 0.17%. Greater percentage of cases fell into the less than 10 years old or more than 40 years old groups. The male to female ratio was 1∶1.46, and 38 cases occurred in farmers. Geographic distribution was remarkable, especially around the Chikugo and Yabe river. Sixty-four cases showed the cutaneous lymphangitic type and 85 cases the localized cutaneous type and one case atypical type. The face and upper extremities were the most affected. The sporotrichin test was positive in 117 of 131 cases. The causative organism was demonstrated in tissue sections in 69% of the cases.
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