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  • Springer eBooks  (3,540)
  • Neurologie
  • Theoretische Physik
  • Messtechnik
  • Strömungsmechanik
  • Springer  (3,574)
  • Oxford University Press
  • American Institute of Physics (AIP)
  • 1
    Keywords: Neurology ; Neurobiology ; Neurology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Integrative Biomarkers in Stroke -- Vascular-Related Biomarkers of Ischemic Stroke -- Generation and Applicability of Genetic Risk Scores (GRS) in Stroke -- Neuroplasticity Biomarkers in Experimental Stroke Recovery -- Methods of Mitochondrial and Redox Measurements in Ischemic Stroke -- Blood Biomarkers for Stroke Differentiation -- Laser-Capture Micro-Dissection for Measurement of Angiogenesis after Stroke -- Blood-Borne Biomarkers of Hypertension Predicting Hemorrhagic and Ischemic Stroke -- RNA Gene Expression to Identify the Etiology of Acute Ischemic Stroke: The Biomarkers of Acute Stroke Etiology (BASE) Study -- Neurotoxicity Biomarkers Assays Development -- Glutamate Receptor Peptides as Potential Neurovascular Biomarkers of Acute Stroke -- Antibodies to NMDA Receptors in Cerebral and Spinal Cord Infarctions -- Impaired Retinal Vasoreactivity as an Early Marker of Stroke Risk in Diabetes -- Imaging Biomarkers: Keys to Decision Making in Stroke -- Neuroimaging Methods for Acute Stroke Diagnosis and Treatment -- Ultrasound Assessments of Risk for TIA and Stroke in Vascular Surgery -- Preoperative and Intraoperative Markers of Cerebral Ischemia -- Time is Brain: the Prehospital Phase and the Mobile Stroke Unit -- Acute Clinical Intervention and Chronic Management of Cerebral Vascular Accident -- Trends in Biomarkers Development for Stroke
    Abstract: This volume presents the latest data on recent achievements in new and emerging technologies for stroke biomarkers and innovations in stroke assessment. The topics discussed in this book explore the role of upcoming biomarkers in different types of stroke, and explores techniques that will allow researchers to be more effective when approaching clinical management and patient care. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to ensure successful results in the laboratory. Cutting-edge and comprehensive, Stroke Biomarkers is a valuable resource for both experimental and clinical scientists interested in expanding their knowledge in the field of stroke research
    Pages: XVII, 425 p. 80 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493996827
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  • 2
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
    Abstract: The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
    Pages: XI, 247 p. 30 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998654
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  • 3
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook -- Immunophenotyping by Mass Cytometry -- Fluorescent Cell Barcoding for Immunophenotyping -- Immunophenotyping using Dried and Lyophilized Reagents -- Guidelines for Gating Flow Cytometry Data for Immunological Assays -- Strategies and Techniques for NK Cell Phenotyping -- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue -- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells -- Immunophenotyping of Human Regulatory T Cells -- Immunophenotyping of Human Innate Lymphoid Cells -- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis -- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles -- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells -- Quality Control of Immunophenotyping -- Immunophenotyping of Acute Myeloid Leukemia -- Immunophenotyping of Acute Lymphoblastic Leukemia -- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia -- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
    Abstract: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field
    Pages: X, 356 p. 94 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493996506
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  • 4
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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  • 5
    Keywords: Microbial genetics ; Microbial genomics ; Bacteriology ; Microbial Genetics and Genomics ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples -- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health -- Detection, Isolation, and Characterization of Plasmids in the Environment -- Bacteriophage Isolation and Characterization: Phages of Escherichia coli -- Detection and Characterization of Transposons in Bacteria -- Measuring Plasmid Conjugation Using Antibiotic Selection -- Measuring Plasmid Conjugation Using Fluorescent Reporters -- Methods to Quantify DNA Transfer in Enterococcus -- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis -- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases -- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis -- First Biochemical Steps on Bacterial Transposition Pathways -- Natural Transformation in Escherichia coli -- Integron Identification in Bacterial Genomes and Cassette Recombination Assays -- Methods to Identify and Analyze Vesicle-Protected DNA Transfer -- Measuring Plasmid Stability in Gram-Negative Bacteria -- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems -- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements -- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan -- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS) -- MOBscan: Automated Annotation of MOB Relaxases -- Plasmid Typing and Classification -- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets -- Statistical Analysis of Accessory Genome -- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits -- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria -- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria -- Conjugative Assembly Genome Engineering (CAGE)
    Abstract: This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality
    Pages: XV, 413 p. 62 illus., 52 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998777
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  • 6
    Keywords: Proteins  ; Biotechnology ; Nanotechnology ; Protein Science ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Introduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins
    Abstract: This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field
    Pages: XIII, 331 p. 84 illus., 64 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998692
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  • 7
    Keywords: Biochemistry ; RNA-ligand interactions ; Protein Science ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Predicting Protein-Protein Interactions using SPRINT -- Automated Extraction and Visualization of Protein-Protein Interaction Networks and Beyond: A Text Mining Protocol -- Construction of Functional Protein Networks using Domain Profile Associations -- Reconstruction of Protein-Protein Interaction Networks using Homology-Based Search: Application to the Autophagy Pathway of Ageing in Podospora anserina -- Predicting Interacting Protein Pairs by Co-Evolutionary Paralog Matching -- A Web-Based Protocol for Inter-Protein Contact Prediction by Deep Learning -- Visual Analysis of Protein-Protein Interaction Docking Models using COZOID Tool -- Path-LZerD: Predicting Assembly Order of Multimeric Protein Complexes -- Embedding Alternative Conformations of Proteins in Protein-Protein Interaction Networks -- Informed Use of Protein-Protein Interaction Data: A Focus on the Integrated Interactions Database (IID) -- Generation and Interpretation of Context-Specific Human Protein-Protein Interaction Networks with HIPPIE -- Explore Protein-Protein Interactions for Cancer Target Discovery using the OncoPPi Portal -- Perform Pathway Enrichment Analysis using ReactomeFIViz -- De Novo Pathway Enrichment with KeyPathwayMiner -- De Novo Pathway-Based Classification of Breast Cancer Subtypes -- Vienna Graph Clustering -- On TD-WGcluster - Theoretical Foundations and Guidelines for the User -- An Introductory Guide to Aligning Networks using SANA, the Simulated Annealing Network Aligner
    Abstract: This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: XI, 286 p. 97 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998739
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  • 8
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example
    Abstract: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field
    Pages: XIV, 405 p. 76 illus., 66 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998180
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  • 9
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels
    Abstract: This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 293 p. 46 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998456
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  • 10
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures
    Abstract: This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research
    Pages: XV, 363 p. 72 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493976171
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  • 11
    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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  • 12
    Keywords: Human Genetics ; Proteins  ; Human Genetics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies
    Abstract: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field
    Pages: XII, 445 p. 86 illus., 61 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998531
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  • 13
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Experimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Abstract: This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study
    Pages: XI, 286 p. 65 illus., 53 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997848
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  • 14
    Keywords: Mass spectrometry ; Proteomics ; Mass spectrometry ; Proteomics ; Springer eBooks
    Description / Table of Contents: Introduction to Mass Spectrometry-based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Comparing Peptide Spectra Matches Across Search Engines -- Calculation of False Discovery Rate for Peptide and Protein Identification -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Interpretation of Tandem Mass Spectra of Posttranslationally Modified Peptides -- Solution to Dark Matter Identified by Mass-tolerant Database Search -- Phosphoproteomics Profiling to Identify Altered Signaling Pathways and Kinase-targeted Cancer Therapies -- Mass Spectrometry Based Characterization of Ub- and UbL-modified Proteins -- Targeted Proteomics as a Tool for Quantifying Urine-based Biomarkers -- Data Imputation in Merged Isobaric Labelling-based Relative Quantification Datasets -- Clustering Clinical Data in R -- Review of Issues and Solutions to Data Analysis Reproducibility and Data Quality in Clinical Proteomics -- Review of Batch Effects Prevention, Diagnostics, and Correction Approaches -- Using the Object-oriented PowerShell for Simple Proteomics Data Analysis -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data
    Abstract: The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics. In contrast to the previous editions, this third edition aims to provide the most relevant computational methods, focusing on computational concepts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Data Analysis in Proteomics, Third Edition to ensure successful results in the further study of this vital field
    Pages: X, 445 p. 136 illus., 94 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493997442
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  • 15
    Keywords: Cell Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Purinergic Signaling -- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling -- Agonists and Antagonists for Purinergic Receptors -- Homology Modelling of P2X Receptors -- Using RNA Interference for Purinoceptor Knock Down in vivo -- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos -- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues -- Flow Cytometry of Membrane Purinoreceptors -- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation -- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking -- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer -- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy -- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor -- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse -- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging -- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels -- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader -- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes -- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells -- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages -- Automated Planar Patch-clamp Recording of P2X Receptors -- Controlling Engineered P2X Receptors with Light -- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment -- Assays to Measure Purinoceptor Pore Dilation -- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization -- Measuring Leukocyte Migration to Nucleotides -- Assessment of Cell Adhesion after Purinoceptor Activation
    Abstract: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines
    Pages: XII, 360 p. 82 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997176
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  • 16
    Keywords: Molecular Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent
    Abstract: This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field
    Pages: X, 300 p. 50 illus., 32 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998821
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  • 17
    Keywords: Infectious Diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Tour de Herpes: Cycling through the Life and Biology of HSV-1 -- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology -- Herpes Simplex Virus Growth, Preparation, and Assay -- Engineering HSV-1 Vectors for Gene Therapy -- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- oHSV Genome Editing by Means of galK Recombineering -- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein -- CRISPR/Cas9-Based Genome Editing of HSV -- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH) -- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing -- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals -- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry -- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells -- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot -- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane -- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination -- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1 -- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
    Abstract: This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV
    Pages: XIV, 457 p. 60 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998142
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  • 18
    Keywords: Bacteriology ; Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing of Staphylococcus aureus -- Genetic Manipulations of Staphylococcal Chromosomal DNA -- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology -- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus -- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus -- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology -- Metabolomic Profiling of Staphylococcus aureus -- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells -- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging -- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options -- Animal Models for Drug Development for MRSA
    Abstract: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens
    Pages: XI, 268 p. 35 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998494
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  • 19
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Anesthesiology ; Neurosciences ; Anesthesiology ; Springer eBooks
    Description / Table of Contents: The Challenge of Accidental Awareness during General Anesthesia -- Impact of Anesthetics on Brain Electrical Activity and Principles of pEEG-Based Monitoring during General Anethesia -- Perioperative Monitoring of Autonomic Nervous Activity -- Monitoring Cerebral Oximetry by Near Infrared Spectroscopy (NIRS) in Anaesthesia and Critical Care: Progress and Perspectives -- Post-Traumatic Stress Disorder following Intraoperative Awareness -- Mechanisms of Action of Inhaled Volatile General Anesthetics: Unconsciousness at the Molecular Level -- Intravenous Hypnotic Agents: From Binding Sites to Loss of Consciousness -- Pharmacological Considerations for the Use of General Anesthetics in the Elderly -- Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies -- Postoperative Cognitive Function following General Anesthesia in Children -- The Challenge of Opioid-Free Anesthesia -- New Insights into the Pharmacology of Dexmedetomidine and Open Issues for Neurosurgical Procedures -- Transgenic Mouse Models, General Anesthetics and Alzheimer Disease: Findings from Preclinical Studies -- The Biochemical Basis of Delirium -- Postoperative Delirium and Postoperative Cognitive Dysfunction
    Abstract: This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology. Furthermore, chapters in this book cover technical topics such as preoperative monitoring of autonomic nervous activity; EEG based monitoring of general anesthesia; monitoring cerebral oximetry by near infrared spectroscopy; pharmacological issues of anesthesia interest; and optimization of perioperative course through the latest techniques such as the opioid-free anesthesia. Finally, this book offers a comprehensive and up-to-date discussion on the phenomenon of general anesthesia awareness and its potential psychological sequelae. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, General Anesthesia Research is a valuable resource for novice and expert anesthesiologists who want to learn more about the important advances made in this developing field
    Pages: XIV, 256 p. 15 illus., 14 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998913
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  • 20
    Keywords: Neurobiology ; Developmental Biology ; Neurosciences ; Laboratory animals ; Neurobiology ; Developmental Biology ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra -- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis -- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis -- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System -- Immunostaining of the Embryonic and Larval Drosophila Brain -- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila -- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling -- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update -- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain -- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila -- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development -- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum -- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium -- X-Ray Microscopy of the Larval Crustacean Brain -- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain -- Immunostainings in Nervous System Development of the Nematode C. elegans -- Methods in Brain Development of Molluscs -- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization -- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum -- In Situ Hybridization and Immunostaining of Xenopus Brain -- Morpholino Studies in Xenopus Brain Development -- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish -- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish -- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain -- Gene Silencing in Chicken Brain Development -- Transplantation of Neural Tissue: Quail-Chick Chimeras -- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development -- The Cre/Lox System to Assess the Development of the Mouse Brain -- In Utero Electroporation to Study Mouse Brain Development
    Abstract: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain
    Pages: XIV, 527 p. 116 illus., 96 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493997329
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  • 21
    Keywords: Antibodies ; Pharmacology ; Antibodies ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Overview of the Current ADC Discovery Landscape -- Pushing the Envelope: Advancement of ADCs Outside of Oncology -- Conjugations to Endogenous Cysteine Residues -- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies -- Transglutaminase-Mediated Conjugations -- Click Chemistry Conjugations -- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates -- Bridged Cysteine Conjugations -- Antibody Conjugations via Glycosyl Remodeling -- ADC Analysis by Hydrophobic Interaction Chromatography -- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs -- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction -- Analysis of ADCs by Native Mass Spectrometry -- High Resolution Characterization of ADCs by Orbitrap LCMS -- Conjugation Site Analysis by MS/MS Protein Sequencing -- Conjugation Site Analysis of Lysine-Conjugated ADCs -- Characterization of ADCs by Capillary Electrophoresis -- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry -- Purification of ADCs by Hydrophobic Interaction Chromatography -- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations -- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions -- Biophysical Methods for Characterization of Antibody Drug Conjugates -- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines -- LC/MS Methods for Studying Lysosomal ADC Catabolism -- Assessing ADC Plasma Stability by LC/MS Methods -- Determination of ADC Concentration by Ligand-Binding Assays
    Abstract: This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the €œáctivation barrieŕ€ when undertaking a new discipline, and provides a ́€œtoolbox́€ for the next generation of ADC scientists
    Pages: XI, 373 p. 138 illus., 109 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999293
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  • 22
    Keywords: Biomedical Engineering ; Cell Biology ; Bioinformatics ; Biology—Technique ; Systems Biology ; Biological systems ; Biomedical Engineering/Biotechnology ; Cell Biology ; Computational Biology/Bioinformatics ; Biological Techniques ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB: Image Analysis & Brownian Motion -- Resolving the process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM)
    Abstract: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images
    Pages: X, 170 p. 69 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030223861
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  • 23
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 24
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 25
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Protective Responses of Different Organs to Environmental Toxicants or Stresses -- Avoidance Behaviour of Nematodes to Environmental Toxicants or Stresses -- Intestinal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Epidermal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Toxicity Induction in Intestine and Epidermis in Nematodes Exposed to Environmental Toxicants or Stresses -- Toxicity Induction in Neurons and Muscle in Nematodes Exposed to Environmental Toxicants or Stresses -- Reproductive Toxicity Induction in Nematodes Exposed to Environmental Toxicants or Stresses -- Intestinal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Epidermal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Neuronal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Germline Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses
    Abstract: This book introduces readers to intestinal and epidermal barriers, and to toxicity induction of environmental toxicants or stresses in the intestine, epidermis, neurons, muscle, and reproductive organs in Caenorhabditis elegans. In addition, it discusses the protective responses of various organs and nematodes’ avoidance behaviour with regard to environmental toxicants or stresses. The intestinal, epidermal, neuronal, and germline signalling pathways required for the regulation of toxicity of environmental toxicants or stresses are also introduced and discussed. As a classic model animal with well-described genetic and developmental backgrounds, the nematode Caenorhabditis elegans has been successfully and widely used in both toxicity assessments and toxicological studies on various environmental toxicants and stresses. Once exposure to certain environmental toxicants has occurred, the toxicants can enter into the primary targeted organs (such as intestinal cells), and even be translocated into secondary targeted organs (such as reproductive organs and neurons). Based on related available data, this book provides a systematic understanding of target organ toxicology in C. elegans
    Pages: XIII, 337 p. 232 illus., 175 illus. in color. : online resource.
    ISBN: 9789811360107
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  • 26
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 27
    Keywords: Plant breeding ; Plant genetics ; Agriculture ; Genetic Engineering ; Alternative Medicine ; Pharmaceutical technology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Agriculture ; Genetic Engineering ; Complementary & Alternative Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Foreword by Prof. M. Iqbal Choudhary, Director, International Center for Chemical and Biological Sciences -- Marine and Freshwater Organisms in Drug Discovery -- Bioactive Molecules from Malaysian Flora -- Immunomosulatory Effect of Cycloartane-Type Triterpenes -- Antigiardial Actitivty of Citrullus lanatus Extracts -- Potential Idiosyncratic Liver Toxicity of Herbal Products -- Antitrypanosomal Activity of Nigerian Plants -- Alkaloids Isolated from Malaysian Plant Families -- Pharmacological Studies on Ficus Species -- Herbals in the treatment of Salmonella typhi -- Biology of Proline-Containing Cyclic Peptides -- Plant Extracts and Memory Impairment -- Herbals Used in Heat and Detoxification in Chinese Traditional Medicine -- Secondary Metabolites from Solanaceae of Iran -- Molecular Docking Study on Rauvolfia species -- Drug Development from the farm -- Phytochemical Screening of Sri Lankan Plants -- New carbohydrate esters of cinnamic acid from the leaves of Gleditschia -- Herbals in the Treatment of Alzheimer's disease
    Abstract: Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the “scientific methods”. Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, it will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture
    Pages: XX, 385 p. 67 illus., 45 illus. in color. : online resource.
    ISBN: 9783030044084
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  • 28
    Keywords: Stem Cells ; Gene Therapy ; Oncology ; Gastroenterology ; Stem Cells ; Gene Therapy ; Cancer Research ; Gastroenterology ; Springer eBooks
    Description / Table of Contents: 1. Introduction -- 2. Stem cells principles and biology -- 3. The truth behind esophagus: the stem cells significance -- 4. Pancreatic Diseases: The Role of Stem Cells -- 5. Stem cells therapy for liver diseases -- 6. The role of stem cells in colorectal cancer carcinogenesis and treatment -- 7. THE ROLE OF STEM CELLS IN THE TREATMENT OF ANAL FISTULAS -- 8. Stem cells in inflammatory bowel disease: From pathogenesis to clinical practice -- 9. Paneth Cells Physiology and Pathophysiology in Inflammatory Bowel Disease
    Abstract: This book reviews recent knowledge of the role of stem cells in the gastrointestinal system. It covers extensive topics for each organ, including the pancreas, esophagus, liver, and colon, while also discussing the contributions of stem cells to therapeutic approaches toward gastrointestinal diseases, including inflammatory bowel diseases. Comprehensive and cutting-edge, Digestive System Diseases: Stem Cell Mechanisms and Therapies deepens a reader’s theoretical expertise in gastrointestinal stem cell biology. It furthers scientists’ understanding of gastrointestinal stem cells and, most importantly, the development of novel therapeutic targets. Graduate and postdoctoral students, medical doctors (including gastroenterologists and surgeons), and principal investigators in both academia and industry will benefit from this book. In particular, it is a valuable resource for professionals within the fields of gastrointestinal research, pharmaceutical science, molecular biology, regenerative medicine, and genetics
    Pages: XIII, 187 p. 16 illus., 15 illus. in color. : online resource.
    ISBN: 9783030119652
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  • 29
    Keywords: Bioinformatics ; Genetics ; Human Genetics ; Agriculture ; Bioinformatics ; Genetics and Genomics ; Human Genetics ; Agriculture ; Springer eBooks
    Description / Table of Contents: Introduction to biological data -- Computers and bioinformatics -- Sequence biological databases -- Biological databases -- Other biological databases -- Nucleic acid sequencing -- Tools and methods in analysis of simple sequences -- Tools and methods in analysis of complex sequences -- Structural bioinformatics -- Protein structure annotations -- Introduction to functional bioinformatics -- Biological networks – tools, methods and analysis -- Metabolomics -- Drug discovery: concepts and approaches -- Drug designing methods and analysis -- Molecular molecular docking -- Insilico pcr -- Modeling and optimization of molecular bio-systems to generate predictive models -- Index
    Abstract: Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it mostly focuses on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. Unlike other bioinformatics books which are mostly theoretical, this book provides practical examples for the readers on state-of-the-art open source tools to solve biological problems. Flow charts of experiments, graphical illustrations, and mock data are included for quick reference. Volume I is therefore an ideal companion for students and early stage professionals wishing to master this blooming field
    Pages: XIV, 405 p. 193 illus., 186 illus. in color. : online resource.
    ISBN: 9783030026349
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  • 30
    Keywords: Medical genetics ; Medicine ; Biochemistry ; Neurosciences ; Gene Function ; Molecular Medicine ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Chapter 1. Molecular and Functional Properties of Progranulin -- Chapter 2. Progranulin as a biomarker for neurodegenerative diseases -- Chapter 3. PGRN and FTLD -- Chapter 4. PGRN and neurodegenerative diseases other than FTLD -- Chapter 5. Progranulin Regulations of Lysosomal Homeostasis and its Involvement in Neurodegenerative Diseases -- Chapter 6. Molecular and Functional Properties of Progranulin -- Chapter 7. PGRN and neuroinflammation -- Chapter 8. Neural Stem/Progenitor Cells and Progranulin -- Chapter 9. Generation and phenotyping of progranulin-deficient mice -- Chapter 10. Pleiotropic protective effects of progranulin in the treatment of ischemic stroke -- Chapter 11. New therapeutic approaches against ocular diseases
    Abstract: This book presents the latest knowledge on the roles of progranulin (PGRN) in normal physiology and pathology and explores the emerging significance of PGRN as a therapeutic target and biomarker in various CNS disorders, including frontotemporal lobe degeneration and other neurodegenerative diseases. Following initial recognition of the importance of PGRN in sexual differentiation of the developing brain and adult neurogenesis, it was subsequently discovered that PGRN acts as a chaperone of lysosomal enzymes and plays a crucial role in maintaining cellular protein homeostasis. It has also been found that sex steroids modulate the expression of PGRN and its trophic effects in the developing CNS and that PGRN directly or indirectly influences neural stem and progenitor cells. Against this background, deeper understanding of the molecular and functional properties of PGRN would provide fresh impetus for the development of mechanism-based therapeutic approaches for multiple disorders. Medications targeting the recovery of lysosomal function appear to hold particular promise in patients with neurodegenerative diseases resulting from PGRN insufficiency. In examining multiple aspects of this fascinating field, the book will be of high value for researchers and graduate students
    Pages: VII, 183 p. 37 illus., 13 illus. in color. : online resource.
    ISBN: 9789811361869
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  • 31
    Keywords: Entomology ; Plant breeding ; Plant anatomy ; Biomedical Engineering ; Mycology ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Preface -- Biodiversity of Fungi from Extreme Habitats and Its Industrial Applications -- Rhizospheric Fungi Isolated from Different Plants and Potential Applications in Diverse Sectors -- Endophytic Fungi from Different Niches with Potential Industrial Importance -- Marine Fungal White Biotechnology -- Aspergillus Biodiversity, Ecological Significance, and Industrial Applications -- Fusarium Biodiversity, Ecological Significance, and Industrial Applications -- Penicillium Biodiversity, Ecological Significance, and Industrial Applications -- Trichoderma Biodiversity, Ecological Significance, and Industrial Applications -- Thermostable Enzymes from Fungi Isolated from Hot Springs and Its Applications -- Cold Active Enzymes from Psychrophilic Fungi and Its Applications -- Industrially Important Enzymes from White-Rot Fungi or Filamentous Fungi -- Fungal Enzymes for the Textile Industry -- Fungal Laccase -- Fermentation Process for Production of Fungal Cellulases -- Fungal Lipases -- Fungal Community and Eco-Friendly Bioresources for Novel Secondary Metabolites -- Agriculturally Important Fungi -- Fungal White Biotechnology in Biosurfactants -- Synthetic Biology and a Novel Approach for Pharmaceutically Important Compounds -- Global Scenario of Fungal White Biotechnology -- Appendixes -- Index --
    Abstract: White biotechnology, or industrial biotechnology as it is also known, refers to the use of living cells and/or their enzymes to create industrial products that are more easily degradable, require less energy, create less waste during production and sometimes perform better than products created using traditional chemical processes. Over the last decade considerable progress has been made in white biotechnology research, and further major scientific and technological breakthroughs are expected in the future. Fungi are ubiquitous in nature and have been sorted out from different habitats, including extreme environments (high temperature, low temperature, salinity and pH), and may be associated with plants (epiphytic, endophytic and rhizospheric). The fungal strains are beneficial as well as harmful for human beings. The beneficial fungal strains may play important roles in the agricultural, industrial, and medical sectors. The fungal strains and their products (enzymes, bioactive compounds, and secondary metabolites) are very useful for industry (e.g., the discovery of penicillin from Penicillium chrysogenum). This discovery was a milestone in the development of white biotechnology as the industrial production of penicillin and antibiotics using fungi moved industrial biotechnology into the modern era, transforming it into a global industrial technology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors, providing both high value nutraceutical and pharmaceutical products. The fungal strains and bioactive compounds also play an important role in environmental cleaning. This volume covers the latest developments and research in white biotechnology with a focus on diversity and enzymes
    Pages: XIX, 571 p. 108 illus., 61 illus. in color. : online resource.
    ISBN: 9783030104801
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  • 32
    Keywords: Stem Cells ; Ophthalmology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Ophthalmology ; Springer eBooks
    Description / Table of Contents: Advantages of the Eye as a Target Organ for Cell-Based Therapy in the Central Nervous System -- Proof of Principle: Preclinical Data on Retinal Cell Transplantation -- Clinical and Pathological Features of Selected Human Retinal Degenerative Diseases -- Degenerative Retinal Diseases: Cells sources for cell-based therapy -- Manufacturing of Clinical Grade Cellular Products Under Gmp Conditions -- Regulatory Requirements for Cell-Based Therapy for Degenerative Retinal Disease -- Biomaterials and Scaffolds for Cell Replacement Therapy -- Restoration of cone photoreceptor function in retinitis pigmentosa (RP) – retinal cell-based therapy -- Cell Delivery: Surgical Approaches -- Diagnosis and Complementary Examinations -- Cell Therapy for Degenerative Retinal Disease: special focus on cell fusion-mediated regeneration -- Clinical Trials of Retinal Cell Therapy -- Future Directions -- Index
    Abstract: This book discusses why specific diseases are being targeted for cell-based retinal therapy, what evidence exists that justifies optimism for this approach, and what challenges must be managed in order to bring this technology from the laboratory into routine clinical practice. There are a number of unanswered questions (e.g., surgical approach to cell delivery, management of immune response, optimum cell type to transplant) that very likely are not going to be answered until human trials are undertaken, but there is a certain amount of “de-risking” that can be done with preclinical experimentation. This book is essential reading for scientists, clinicians, and advanced students in stem cell research, cell biology, and ophthalmology
    Pages: XI, 280 p. 27 illus., 20 illus. in color. : online resource.
    ISBN: 9783030052225
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  • 33
    Keywords: Medicine ; Orthopedics ; Molecular Medicine ; Orthopedics ; Springer eBooks
    Description / Table of Contents: Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone
    Abstract: This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research
    Pages: XV, 670 p. 172 illus., 121 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493989973
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  • 34
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 35
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 36
    Keywords: Medicine ; Biomedicine general ; Springer eBooks
    Description / Table of Contents: Approach and Nature of Palliative Care -- Quality of Life -- Global Aspects of Palliative Care -- Challenges and Future Directions of Palliative Care -- Organization of Palliative Care in Different Parts of the World -- Policies on Palliative Care in Different Parts of the World -- Palliative Care, Towards a New Definition -- Physical, Psychological/Psychiatric, Social and Spiritual Problems and Symptoms -- Pain and Pain Management -- Palliative Management of Breathlessness -- Fatigue -- Edema and Lymphedema -- Gastrointestinal Symptoms: Constipation, Diarrhea, and Intestinal Obstruction -- Gastrointestinal Symptoms: Nausea and Vomiting -- Cachexia/Anorexia and Feeding Difficulties -- Mouth -- Skin Symptoms -- Swallowing Difficulties -- Sleep Difficulties -- Psychological Symptoms -- Delirium and Palliative Care -- Neurological Symptoms -- Genitourinary Symptoms -- End-of-life Symptoms -- Gastroparesis and Cancer-Related Gastroparesis -- Advance Care Planning in Palliative Care -- Prognostication in Palliative Care -- Spirituality in Palliative Care -- Access to Palliative Care -- Integrated Palliative Care: Clinical, Organizational, and Health System Perspectives -- Family Focused Care Span -- Grief and Bereavement -- Rehabilitation in Palliative Care -- Dietetics and Nutrition in Palliative Care -- Mouth Care -- Creative Art Making in Palliative Care -- Music Engagement and Therapeutic Music -- Sexuality and Intimacy -- Interprofessional Practice in Palliative Care -- Generalists and Specialist Palliative Care -- Nursing and Palliative Care -- Self-Care and Palliative Care -- End of Life Care -- Self-management in Palliative Care -- Education and Palliative Care, Overview -- Palliative Care in Rural Settings -- Home Care, Primary Care -- Palliative Care in Residential Settings -- Hospital Care -- Palliative Care in the Intensive Care Unit (ICU) -- Volunteers in Palliative Care -- Palliative Care and Cancer -- Palliative Care and Neurodegenerative Diseases -- Palliative Care and Stroke -- Palliative Care in Heart Failure -- Palliative care of Respiratory Disease in Primary Care -- Palliative Care and Liver Diseases -- Palliative Care in Kidney Disease -- Palliative Care and Endocrine Diseases -- Palliative Care in Chronic Illness and Multimorbidity -- Palliative care in dementia -- Dealing with a Wound in Palliative Care -- Pressure Area Management in Palliative care -- End of Life Health Care Experiences of Indigenous People and Ethnic Minorities; the example of Canada -- Palliative Care, Frailty and Older People -- Palliative Care of Pediatric Populations -- Palliative Care and Intellectual Disability -- Palliative Care of Indigenous Peoples and Peoples of Different Ethnicity -- Hypercalcemia of Malignancy -- Thromboembolism and bleeding -- Spinal Cord Compression -- Superior Vena Cava Obstruction -- Acute Cancer Pain Syndromes in Palliative Care Emergencies -- Acute Dyspnoea -- Neutropenic Sepsis -- Seizures -- Tumor Lysis Syndrome -- Suicide and Attempted Suicide -- Transfer to a Preferred Place of Death -- Challenging Family Dynamics -- Delirium as a Palliative Care Emergency -- Distinguishing and Managing Severe Psychological and Psychiatric Distress -- End-Of-Life Decisions -- Sedation and Terminal Sedation -- Nutrition and Hydration in Palliative Care -- Ethics of home-coming -- Request for Assisted Suicide -- Public Health and Epidemiological Research in Palliative Care -- Development and Evaluation of Complex Interventions in Palliative Care -- Mixed Method Research in Palliative Care -- Ethics in Palliative Care Research -- Evidence based Practice in Palliative Care -- New Public Health Approaches to End of Life Care -- A public health approach to integrate palliative care into a country’s health care system: guidance as provided by the WHO -- Measuring Cost-Effectiveness in Palliative Care -- Financial Aspects of Inpatient Palliative Care -- Financial Aspects of Outpatient Palliative Care -- Serious Illness and Out-of-Pocket Spending
    Abstract: Textbook of Palliative Care is a comprehensive, clinically relevant and state-of-the art book, aimed at advancing palliative care as a science, a clinical practice and as an art. Palliative care has been part of healthcare for over fifty years but we still find ourselves having to explain its nature and practice to colleagues and to the public in general. Healthcare education and training has been slow to recognize the vital importance of ensuring that all practitioners have a good understanding of what is involved in the care of people with serious or advanced illnesses and their families. However, the science of palliative care is advancing and our understanding concerning many aspects of palliative care is developing rapidly. The book is divided into separate sections for ease of use. Over 100 chapters written by experts in their given fields provide up-to-date information on a wide range of topics of relevance to those providing care towards the end of life no matter what the disease may be. We present a global perspective on contemporary and classic issues in palliative care with authors from a wide range of disciplines involved in this essential aspect of care. The Textbook includes sections addressing aspects such as symptom management and care provision, organization of care in different settings, care in specific disease groups, palliative care emergencies, ethics, public health approaches and research in palliative care. This Textbook will be of value to practitioners in all disciplines and professions where the care of people approaching death is important, specialists as well as non-specialists, in any setting where people with serious advanced illnesses are residing. It is also an important resource for researchers, policy-and decision-makers at national or regional levels. Neither the science nor the art of palliative care will stand still so we aim to keep this Textbook updated as the authors find new evidence and approaches to care
    Pages: 194 illus., 161 illus. in color. eReference. : online resource.
    ISBN: 9783319777405
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  • 37
    Keywords: Biomedical Engineering ; Nanotechnology ; Cytology ; Bioethics ; Biomedical Engineering/Biotechnology ; Nanotechnology ; Oxidative Stress ; Bioethics ; Springer eBooks
    Description / Table of Contents: Novel therapeutics and diagnostics strategies based on engineered nanobiomaterials -- Gold Nanostructures for Photothermal Therapy -- Nanomaterials-based siRNA Delivery: Routes of administration, hurdles and role of nanocarriers -- Silver-based Polymeric Nanocomposites as Antimicrobial Coatings for Biomedical Applications -- Recent trends of nano-materials antimicrobial agents -- Therapeutic Leishmaniasis: Recent Advancement and Developments -- Nanomanipulation of consumer goods: Effects on human health and environment
    Abstract: The book introduces the basic concepts of nanotechnology and the various technologies to characterize nanomaterials. It also covers the nanostructural features of mammalian cells/tissues and related nanomechanical properties. In addition, the book comprehensively describes the current state-of-the-art and future perspectives of nanotechnology in biosensors. It also discusses the potential of nanotechnology for delivering the diverse cancer therapeutics and illustrates its limitation due to the potential toxicity associated with oxidative stress. It also highlights the ethical issues and translational aspects related to nanotechnology. Finally, it summarizes the applications of nanotechnology in animal biotechnology, the recent perspectives and future challenges of nanomedicines. The content of the book are beneficial for the undergraduate, postgraduate and doctoral students as well the professionals working in the area of nanotechnology and nanomedicines
    Pages: XII, 254 p. 40 illus., 30 illus. in color. : online resource.
    ISBN: 9789811360046
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  • 38
    Keywords: Emerging infectious diseases ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media -- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell -- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell -- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome -- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host -- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome -- Gene Expression Analysis in the Pneumococcus -- Transcriptional Knockdown in Pneumococci using CRISPR Interference -- Protein Expression Analysis by Western Blot and Protein-Protein Interactions -- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony -- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria -- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells -- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae -- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease -- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection -- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo -- The Pneumococcus and Its Critical Role in Public Health -- The Epidemiology and Biostatistics of Pneumococcus
    Abstract: This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field
    Pages: XV, 227 p. 37 illus., 26 illus. in color. : online resource.
    ISBN: 9781493991990
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  • 39
    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 40
    Keywords: Stem Cells ; Oncology ; Cell Cycle ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Cell Cycle Analysis ; Springer eBooks
    Description / Table of Contents: Stem cells heterogeneity -- Heterogeneity in epiblast stem cells -- Progenitor cell heterogeneity in the adult carotid body germinal niche -- Planarian stem cell heterogeneity -- The cellular origin of Barrett’s esophagus and its stem cells -- Pluripotent stem cell heterogeneity -- Sarcoma stem cell heterogeneity -- Heterogeinity in adipose derived stem cells -- Unveiling stem cell heterogeneity towards the development of salivary gland regenerative strategies -- Heterogeneity of human mesenchymal stromal/stem cells -- Mitochondrial heterogeneity in stem cells -- The heterogeneity of renal stem cells, and their interaction with bio- and nano-materials -- Index
    Abstract: This book presents a comprehensive discussion on the novel concepts in stem cell heterogeneity, from pluripotent stem cells to human mesenchymal stem cells, adult and cancer stem cells of the thyroid, sarcoma, and more. Thus, Stem Cell Heterogeneity - Novel Concepts starts from a timely update on the current information on stem cells heterogeneity in various tissues and discusses new concepts and future directions. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity in Different Organs, and Stem Cells Heterogeneity in Cancer, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians
    Pages: XII, 225 p. 45 illus., 38 illus. in color. : online resource.
    ISBN: 9783030110963
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  • 41
    Keywords: Human Physiology ; Neurosciences ; Cytology ; Radiology, Medical ; Stem Cells ; Nanotechnology ; Human Physiology ; Neurosciences ; Oxidative Stress ; Diagnostic Radiology ; Stem Cells ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Cerebral stroke: An Introduction -- Chapter 2. Inflammation, oxidative stress, and neurodegeneration -- Chapter 3. Stroke induced blood brain barrier damage -- Chapter 4. Regulation of calcium ions in ischemic neuronal cell -- Chapter 5. Ischemic stroke induced endoplasmic reticulum stress -- Chapter 6. The role of autophagy in ischemic stroke: friend or foe?- Chapter 7. Critical role of mitochondrial autophagy in cerebral stroke -- Chapter 8. Application of neuroimaging in the identification of the pinpoint location of blockage -- Chapter 9. Emerging role of the electromagnetic field in stroke -- Chapter 10. Stem cell therapies for stroke -- Chapter 11. MicroRNA: Significance to stroke diagnosis, prognosis, and therapy -- Chapter 12. Therapeutic Aspects of Nanomedicines in Stroke Treatment -- Chapter 13. Neuroprotective potential of small molecule phytochemicals against stroke -- Chapter 14. Role of UV irradiation on neuroprotective potential of phytochemicals -- Chapter 15. Post-stroke treatment strategies, management, and rehabilitation
    Abstract: This book provides detailed and comprehensive mechanistic insights of the various risk factors that lead to the ischemic stroke and the novel therapeutic interventions against it. The first section discusses the different ischemic cerebral stroke-induced inflammatory pathways and dysfunctionality of blood-brain barrier. The later sections of the book deals with the role of endoplasmic reticulum stress and mitophagy in cerebral stroke and introduces the different neuroimaging techniques such as Computed tomography (CT), Magnetic resonance imaging (MRI), Positron emission tomography (PET) and Single-Photon emission computed tomography (SPECT) that are used to identify the arterial blockages. The final section comprises of chapters that focus on various neuroprotective strategies and emerging therapeutic interventions for combating stroke pathophysiology. The chapters cover the role of stem cell therapy, the therapeutic effect of low-frequency electromagnetic radiations (LF-EMR), and implications of non-coding RNAs such as micro-RNAs as the biomarkers for diagnosis, prognosis, and therapy in ischemic stroke
    Pages: VIII, 189 p. 23 illus., 12 illus. in color. : online resource.
    ISBN: 9789811314537
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  • 42
    Keywords: Microbiology ; Microbial ecology ; Marine Sciences ; Microbiology ; Microbial ecology ; Marine & Freshwater Sciences ; Springer eBooks
    Abstract: This book discusses how aquatic microbial communities develop interactive metabolic coordination both within and between species to optimize their energetics. It explains that microbial community structuration often includes functional stratification among a multitude of organisms that variously exist either suspended in the water, lodged in sediments, or bound to one another as biofilms on solid surfaces. The authors describe techniques that can be used for preparing and distributing microbiologically safe drinking water, which presents the challenge of successfully removing the pathogenic members of the aquatic microbial community and then safely delivering that water to consumers. Drinking water distribution systems have their own microbial ecology, which we must both understand and control in order to maintain the safety of the water supply. Since studying aquatic microorganisms often entails identifying them, the book also discusses techniques for successfully isolating and cultivating bacteria. As such, it appeals to microbiologists, microbial ecologists and water quality scientists
    Pages: XIII, 351 p. 100 illus., 38 illus. in color. : online resource.
    ISBN: 9783030167752
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  • 43
    Keywords: Toxicology ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Complexity of Biologics CMC Regulation -- Biopharmaceutics are Not Chemical Drugs -- An Effective CMC Strategy is Possible -- Challenge of Adventitious Agent Control -- Biopharmaceutical Source Materials -- Manufacturing of Biopharmaceutical APIs -- Manufacturing of the Drug Product -- Complex Process-Related Impurity Profiles -- Product Characterization is a Journey -- Priceless Potency (Therapeutic Activity) -- Quality Attributes of a Biopharmaceutical -- Designing the Stability Program -- The Art of Setting Specifications -- Demonstrating Product Comparability After Process Changes -- Invaluable CMC-Focused Meetings with Regulatory Authorities
    Abstract: This book since first published in 2004 has been a major resource providing insights and practical guidance for the CMC teams to develop an acceptable cost-effective, risk-based CMC regulatory compliance strategy for biopharmaceuticals (recombinant proteins, monoclonal antibodies, genetically engineered viruses and genetically engineered human cells) from early clinical stage development through market approval. The third edition of this book provides added coverage for the biosimilars, antibody drug conjugates (ADCs), bispecific antibodies, genetically engineered viruses, and genetically engineered cells. This third edition of the book also addresses the heightened pressure on CMC regulatory compliance timelines due to the introduction of expedited clinical pathways moving the clinical development closer to a seamless phase process (e.g., FDA Breakthrough Therapy designation, CBER Regenerative Medicine Advanced Therapy (RMAT) designation, EMA Priority Medicines (PRIME) designation). The Challenge of CMC Regulatory Compliance for Biopharmaceuticals is essential, practical information for all pharmaceutical development scientists, Manufacturing and Quality Unit staff, Regulatory Affairs personnel, and senior management involved in the manufacture of biopharmaceuticals
    Pages: XXVIII, 426 p. 59 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9783030137540
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  • 44
    Keywords: Neurochemistry ; Biomedical Engineering ; Neurochemistry ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: 1. Past, present and future of neuronal models in vitro -- 2. In vitro models of neural disorders -- 3. Neuronal cultures and nanomaterials -- 4. Advances in Human Stem Cell Derived Neuronal Cell Culturing and Analysis -- 5. Large scale, high-resolution microelectrode arrays for interrogation of neurons and networks -- 6. Multi-site intracellular recordings -- 7. From MEAs to MOAs: the next generation of bioelectronic interfaces for neuronal cultures -- 8. Scaling spike detection and sorting for next generation electrophysiology -- 9. Burst detection methods -- 10. Reconstruction of functional connectivity from multi-electrode recordings and calcium imaging -- 11. Open-source tools for processing and analysis of in vitro extracellular neuronal signals -- 12. Screening chemicals for potential neurotoxicity and developmental neurotoxicity using neural networks grown on MEAs -- 13. Long-term activity dynamics of single neurons and networks -- 14. Electrical and optical closed-loop systems
    Abstract: This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies
    Pages: XV, 387 p. 102 illus., 90 illus. in color. : online resource.
    ISBN: 9783030111359
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    Keywords: Biochemistry ; Toxicology ; Neurobiology ; Protein Science ; Pharmacology/Toxicology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics -- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs -- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies -- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties -- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions -- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands -- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs -- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology -- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches -- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays -- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions -- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters -- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays -- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay -- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry -- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy -- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies -- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy -- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy -- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug -- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice -- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents -- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs
    Abstract: This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com
    Pages: XV, 406 p. 87 illus., 71 illus. in color. : online resource.
    ISBN: 9781493991211
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  • 46
    Keywords: Neurosciences ; Diabetes ; Neurology ; Neurosciences ; Diabetes ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1 Origins of brain insulin and its function -- Chapter 2 Epidemiological evidence of the relationship between diabetes and dementia -- Chapter 3 Molecular pathophysiology of insulin depletion, mitochondrial dysfunction, and oxidative stress in Alzheimer’s Disease Brain -- Chapter 4 The Full Spectrum of Alzheimer's Disease is Rooted in Metabolic -- Derangements That Drive Type 3 Diabetes -- Chapter 5 The roles of apolipoprotein E, lipids and glucose in the pathogenesis of Alzheimer’s disease -- Chapter 6 Molecular connection between diabetes and dementia -- Chapter 7 Type II Diabetes mellitus accelerates age-dependent A pathology in cynomolgus monkey brain -- Chapter 8 Diabetes-related dementia -- Chapter 9 Tortuous paths of insulin signaling and mitochondria in Alzheimer's disease -- Chapter 10 Mammalian target of rapamycin at the crossroad between Alzheimer’s Disease and diabetes -- Chapter 11 Therapeutic strategies for Alzheimer’s disease in the view of diabetes mellitus
    Abstract: This book describes the precise mechanisms by which insulin resistance and diabetes mellitus (DM) act as risk factors for Alzheimer’s disease (AD). It opens by discussing the de novo synthesis of insulin in the brain and its functional significance with regard to glucose metabolism and maintenance of neuronal function in the brain. The epidemiological evidence that DM is a risk factor for the development of dementia, including AD as well as vascular dementia, is then examined. Subsequent chapters explore in depth the mechanisms involved in this relationship, including abnormal protein processing, dysregulated glucose metabolism, impaired insulin signaling, and mitochondrial dysfunction. The molecular interactions between diabetes and AD are fully discussed, highlighting the pathological molecular mechanisms induced by diabetes that promote and accelerate AD pathology. Finally, diagnostic biomarkers and potential therapeutic approaches for AD are considered on the basis of the presented evidence. In providing answers to the critical questions of whether and why DM is a risk factor for AD, this book will hold appeal for a wide interdisciplinary audience
    Pages: VI, 248 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9789811335402
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    Unknown
    Singapore : Springer
    Keywords: Medicine ; Biochemistry ; Molecular Medicine ; Protein Science ; Springer eBooks
    Description / Table of Contents: Part 1: Overview of Periostin -- Chapter 1: Naming, History, Future -- Part 2: Basic properties of Periostin -- Chapter 2: The structure of the periostin gene, its transcriptional control and alternative splicing, and protein expression -- Part 3: Function as the scaffold -- Chapter 3: Periostin functions as a scaffold for assembly of extracellular proteins -- Part 4: Health and Disease in Organs -- Chapter 4: Periostin reexpression in heart disease contributes to cardiac interstitial remodeling by supporting the cardiac myofibroblast phenotype -- Chapter 5: Periostin in bone biology -- Chapter 6: Periostin in bone regeneration -- Chapter 7: Functions of periostin in dental tissues and its role in periodontal tissue regeneration -- Chapter 8: Periostin and human teeth -- Chapter 9: Ability of periostin as a new biomarker of idiopathic pulmonary fibrosis -- Chapter 10: Involvement of periostin in skin function and the pathogenesis of skin diseases -- Chapter 11: Periostin in the kidney -- Chapter 12: Periostin in eye diseases -- Chapter 13: The maltiaspect functions of periostin in tumor progression -- Part 5: Other types of fibrosis and tissue repair -- Chapter 14: Liver, Stroke, Rhinosinusitis -- Part 6: Inflammation -- Chapter 15: Roles of periostin in asthma -- Part 7: Periostin in Development -- Chapter 16: Periostin and integrin signaling in stem cell regulation -- Chapter 17: Role of periostin in cardiac valve development -- Part 8: Biomarker -- Chapter 18: Practical application of periostin as a biomarker for pathological conditions -- Part 9: Clinical applications -- Chapter 19: Clinical trials and new applications
    Abstract: This book provides a comprehensive and up-to-date review of current understanding of periostin and its importance for human health and disease. Periostin is a secretory matricellular protein that has been revealed to play key roles in fibrillogenesis and cell migration, including metastasis of cancer cells. The production of periostin is upregulated during fibrotic responses and the mechanisms by which it promotes fibrosis have become a focus of interest owing to the potential clinical benefit to be derived from periostin blockade. In this book, readers will find coverage of all aspects, from the basic properties of periostin and its function as a scaffold for assembly of extracellular proteins through to its roles in bone and tissue regeneration, tumorigenesis, myocardial infarction, inflammatory and immune system disorders, and other diseases. Readers will also find the latest information on functions of periostin related to stemness and the application of periostin as a biomarker. It is hoped that the detailed knowledge of periostin and its pathobiological significance provided in this book will aid in the search for effective treatments for currently incurable diseases
    Pages: VIII, 213 p. 48 illus., 40 illus. in color. : online resource.
    ISBN: 9789811366574
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    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: 1. Classification of protein disulfide bonds -- 2. Assessing the evolutionary conservation of protein disulphide bonds -- 3. A proteomics workflow for the Identification of labile disulphide bonds at the cell surface -- 4. Quantification of the redox state of protein disulfide bonds -- 5. Quantification of the redox state of protein disulfide bonds -- 6. Studying functional disulfide bonds by computer simulations -- 7. Dynamic force spectroscopy analysis on the redox states of protein disulfide bonds -- 8. Assays of thiol isomerase enzymatic activity -- 9. Functional assays of thiol isomerase ERp5 -- 10. IDENTIFICATION OF PDI SUBSTRATES BY MECHANISM-BASED KINETIC TRAPPING -- 11. Site-specific proteomic mapping of modified cysteine residues -- 12. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry -- 13. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry -- 14. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide -- 15. Methodology for identification of the cysteine-reactive covalent inhibitors -- 16. In Vivo Measurement of Redox-Regulated TG2 Activity -- 17. Quantitation of total and free thiol β2-glycoprotein I levels for diagnostic and prognostic purposes in the antiphospholipid syndrome -- 18. Quantitation of total and free thiol angiotensinogen as a prognostic marker for pre-eclampsia -- 19. Preparation of a dithiol-reactive probe for PET imaging of cell death -- 20. Flow Cytometry Assessment of Procoagulant Platelets Using A Dithiol Reactive Probe
    Abstract: This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study
    Pages: XI, 327 p. 124 illus., 69 illus. in color. : online resource.
    ISBN: 9781493991877
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  • 49
    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 50
    Keywords: Genetics ; Biochemistry ; Genetics and Genomics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo -- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System -- Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation -- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts -- High-Throughput Allelic Replacement Screening in Bacillus subtilis -- Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast -- Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes -- Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing -- Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo -- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation -- Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA) -- Preparation of Cell Cycle Synchronized Saccharomyces cerevisiae Cells for Hi-C -- Dissecting DNA Compaction by the Bacterial Condensin MukB -- In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA -- A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes -- In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks -- Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging -- Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus under DNA Damage -- Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes -- Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex -- Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase -- Molecular Dynamics Simulations of Condensin-Mediated Mitotic Chromosome Assembly
    Abstract: This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA. Chapters 3, 10 and 19 are available Open Access under a CC BY 4.0 license via link.springer.com
    Pages: XIII, 336 p. 91 illus., 55 illus. in color. : online resource.
    ISBN: 9781493995202
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