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  • Organic Chemistry  (68,947)
  • Springer eBooks  (6,712)
  • 1
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 2
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 3
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: AcetylCoA Synthesis and Regulation -- AcetylCoA Synthesis and Role in Bacteria -- Autophagy in Stationary Phase of Growth -- Bacterial Sphingolipids -- Cell-contact-dependent Outer Membrane Exchange in Myxobacteria -- Contributions of Membrane Lipids to Bacterial Cell Homeostasis Upon Osmotic Challenge -- Dynamic Membrane Structure: Function Analysis by Means of Chemical Probes -- Evolution of Hydrophobic Storage Polymers -- Evolution of Lipids -- Evolution of Membranes -- Fatty Acid Synthesis and Regulation -- Fatty Acids: Introduction -- Formation of Bacterial Glycerol-based Membrane Lipids: Pathways, Enzymes, Reactions -- Formation of Fatty Acids -- Formation of Hopanoids -- Formation of Isoprenoids -- Formation of Lipochitin Oligosaccharide Signaling Molecules -- Functional Roles of Individual Membrane Lipids in Bacteria, Archaea and Eukaryotic Microbes -- Functional Roles of Non-membrane Lipids in Bacterial Signaling -- Hopanoids and Membrane Integrity and pH Homeostasis -- Lipid A -- Lipid Chaperones -- Lipid Chaperones and Regulation -- Lipid Intermediates in Bacterial Peptidoglycan Biosynthesis -- Lipid Metabolism in Microalgae -- Lipid Rafts -- Lipid-protein Interactions -- Lipid-protein Interactions Determining Membrane Fluidity in Prokaryotes and Eukaryotes -- Lipids as Receptors -- Lipoteichoic Acid Synthesis and Function in Gram-positive Bacteria -- Membrane Disrupting Proteins -- Membrane Formation and Regulation -- Membrane Homeostasis Upon Nutrient (C, N, P) Limitation -- Membrane Homeostasis upon pH Challenge -- Membrane Lipid Biogenesis -- Membrane Structure:Function Analysis by Means of Chemical Probes -- Membrane Structure:Function Analysis Through Reconstitution In Vitro -- Membrane Vesicles, Nanopods and Nanotubes of Archaea -- Metabolism and Regulation of Glycerolipids in Yeast -- Metabolism and Roles of Sphingolipids in Yeast Saccharamyces cerevisiae -- Modeling Lipid Membranes -- Modeling Lipid Metabolism in Yeast -- Mycolic Acids: Structures, Biosynthesis , and Function -- Nonpolar Lipids in Yeast: Synthesis, Storage and Degradation -- Ornithine Lipids and Other Amino Acid-containing Acyloxyacyl Lipids -- Outer Membrane Extension Nanowires of Bacteria -- Outer Membrane Vesicles of Bacteria -- Phenolic Lipids Synthesized by Type III Polyketide Synthases -- Players in the Nonpolar Lipid Game - Proteins Involved in Nonpolar Lipid Metabolism in Yeast -- Polyhydroxyalkanoate Biogenesis -- Preface: Biogenesis of Fatty Acids, Lipids and Membranes -- Production of Wax Esters by Bacteria -- Protein Lipidation, Elucidation by Chemical Proteomics, and its Functional Roles -- Regulation of Membrane Lipid Homeostasis in Bacteria upon Temperature Change -- Role of Lipid Domains in Bacterial Cell Processes -- Role of Lipids in the Eukaryotic Secretory Pathway -- Role of the BAM Complex in Outer Membrane Assembly -- Structure:Function of Transmembrane Domains of Proteins and Transmembrane Organelles -- Synthesis of AcetylCoA from Carbon Dioxide in Acetogenic Bacteria -- Synthesis of Acetyl-CoA from Carbon Dioxide in Acetogenic Bacteria -- The Biosynthesis and Evolution of Archaeal Membranes and Ether Phospholipids -- Type III Polyketide Synthases Responsible for Phenolic Lipid Synthesis -- Vitamin Formation from Fatty Acid Precursors -- Wax Ester and Triacylglycerol Biosynthesis in Bacteria
    Abstract: Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes). All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes
    Pages: 161 illus., 115 illus. in color. eReference. : online resource.
    ISBN: 9783319504308
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  • 4
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single Molecule FRET Methods to Study Glutamate Receptors -- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM) -- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy -- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors -- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function -- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE) -- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus -- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats -- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction -- Gene Expression Analysis by Multiplex Single Cell RT-PCR -- Metabolomics Analysis of Glutamate Receptor Function -- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation -- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function -- Fractionation of Subcellular Compartments from Human Brain Tissue -- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
    Abstract: This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications
    Pages: X, 260 p. 67 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990771
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  • 5
    Keywords: Nucleic Acids ; Genetic Engineering ; Popular Life Sciences ; Nucleic Acid Chemistry ; Genetic Engineering ; Popular Science in Chemistry and Materials ; Springer eBooks
    Abstract: Carolyń€™s parents did not, after all, make genomics history by synthesizing her genome in a lab. She has known she is the "Human Hoax" ever since a high school genetics exercise revealed she has trisomy X€”á chromosomal abnormality€”ýet no synthetically constructed genome would have such clear traces of natural conception. Many years later, as molecular biologist, she hopes her colleagues never learn of her embarrassing origins. But when someone ransacks her office and lab, she finds professional embarrassment is the least of her worries. Someone believes she has the results of her parentś€™ last, secret experiments, and is willing to kill to get them. But all she has from her parents are their geneś€”can she find what else they may have left her before somebody else does? In a not-so-distant society, where corporations wield as much power as nations and the line between corporate employee and state authority is blurred, the chase is on. Carolyn may have just too little time at hand to unravel the mystery of her parentś€™ final days and to realize the deep consequences for the future of mankind. This fast-paced novel is followed by an extensive science chapter where the author provides a non-technical primer on modern genetics and on the speculative biology behind Carolyń€™s code. Dr V Anne Smith is on the biology faculty at the University of St Andrews in Scotland. She researches computational biology, concentrating on complex biological networks. She develops algorithms to infer functional interactions based upon observational data, generating biological discovery in areas as diverse as ecosystems and gene regulation. In addition to doing research, she enjoys writing science fiction stories. A Code for Carolyn is her first scientific novel
    Pages: V, 235 p. 6 illus. : online resource.
    ISBN: 9783030045531
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  • 6
    Keywords: Biomedical Engineering ; Neurosciences ; Radiology, Medical ; Neurology ; Biomedical Engineering/Biotechnology ; Neurosciences ; Diagnostic Radiology ; Mathematical and Computational Biology ; Neurology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Introduction -- Chapter 2. Multifractal study of EEG signal of subjects with Epilepsy and Alzheimer’s -- Chapter 3. Multifractal Approach for Quantification of Autonomic Deregulation due to Epileptic Seizure with ECG data -- Chapter 4. Multifractal Analysis of Electromyography data.-Chapter 5. Multifractal study on Parkinson’s and Huntington diseases with human gait data -- Chapter 6. Multifractal correlation study between posture and autonomic deregulation using ECG and blood pressure data -- Chapter 7. Appendix
    Abstract: This book primarily focuses on the study of various neurological disorders, including Parkinson’s (PD), Huntington (HD), Epilepsy, Alzheimer’s and Motor Neuron Diseases (MND) from a new perspective by analyzing the physiological signals associated with them using non-linear dynamics. The development of nonlinear methods has significantly helped to study complex nonlinear systems in detail by providing accurate and reliable information. The book provides a brief introduction to the central nervous system and its various disorders, their effects on health and quality of life, and their respective courses of treatment, followed by different bioelectrical signals like those detected by Electroencephalography (EEG), Electrocardiography (ECG), and Electromyography (EMG). In turn, the book discusses a range of nonlinear techniques, fractals, multifractals, and Higuchi’s Fractal Dimension (HFD), with mathematical examples and procedures. A review of studies conducted to date on neurological disorders like epilepsy, dementia, Parkinson’s, Huntington, Alzheimer’s, and Motor Neuron Diseases, which incorporate linear and nonlinear techniques, is also provided. The book subsequently presents new findings on neurological disorders of the central nervous system, namely Parkinson’s disease and Huntington’s disease, by analyzing their gait characteristics using a nonlinear fractal based technique: Multifractal Detrended Fluctuation Analysis (MFDFA). In closing, the book elaborates on several parameters that can be obtained from cross-correlation studies of ECG and blood pressure, and can be used as markers for neurological disorders
    Pages: XX, 182 p. 77 illus., 51 illus. in color. : online resource.
    ISBN: 9789811335525
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  • 7
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: I-Cell Disease (Mucolipidosis II): A Case Series from a Tertiary Paediatric Centre Reviewing the Airway and Respiratory Consequences of the Disease -- Oral Ganglioside Supplement Improves Growth and Development in Patients with Ganglioside GM3 Synthase Deficiency -- Feeding Difficulties and Orofacial Myofunctional Disorder in Patients with Hepatic Glycogen Storage Diseases -- Auxiliary Partial Orthotopic Liver Transplantation for Monogenic Metabolic Liver Diseases: Single-Centre Experience -- A Novel Truncating FLAD1 Variant, Causing Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) in an 8-Year-Old Boy -- The Effect of Continuous Intravenous Glucagon on Glucose Requirements in Infants with Congenital Hyperinsulinism -- Case of Neonatal Fatality from Neuromuscular Variant of Glycogen Storage Disease Type IV -- Acute and Chronic Management in an Atypical Case of Ethylmalonic Encephalopathy -- Dihydropyrimidine Dehydrogenase Deficiency: Homozygosity for an Extremely Rare Variant in DPYD due to Uniparental Isodisomy of Chromosome 1 -- Severe Neonatal Manifestations of Infantile Liver Failure Syndrome Type 1 Caused by Cytosolic Leucine-tRNA Synthetase Deficiency -- Enzyme Replacement Therapy in Pregnant Women with Fabry Disease: A Case Series -- Survival of a Male Infant with a Familial Xp11.4 Deletion Causing Ornithine Transcarbamylase Deficiency -- The Unique Spectrum of Mutations in Patients with Hereditary Tyrosinemia Type 1 in Different Regions of the Russian Federation -- Elevated Lyso-Gb3 Suggests the R118C GLA Mutation Is a Pathological Fabry Variant -- Glycogen Storage Disease Type IV: A Rare Cause for Neuromuscular Disorders or Often Missed? -- A Hemizygous Deletion Within the PGK1 Gene in Males with PGK1 Deficiency
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 110 p. 28 illus., 13 illus. in color. : online resource.
    ISBN: 9783662586471
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  • 8
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Plant genetics ; Bacteriology ; Bioinformatics ; Human Genetics ; Microbial Genetics and Genomics ; Microbial ecology ; Plant Genetics and Genomics ; Bacteriology ; Bioinformatics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part 1. Concepts and Approaches -- Chapter1. Computational Methods in Microbial Population Genomics -- Chapter2. What Microbial Population Genomics has taught us about Speciation -- Chapter3. Peering into the genetic makeup of natural microbial populations using metagenomics -- Chapter4. A reverse ecology framework for bacteria and archaea -- Part2. Population genomics of bacteria and archaea -- Chapter5. What is a Pseudomonas syringae Population? -- Chapter6. An introductory narrative to the population genomics of pathogenic bacteria, exemplified by Neisseria meningitidis -- Chapter7. Population genomics of archaea: Signatures of archaeal biology from natural populations -- Part3. Population genomics of fungi -- Chapter8. Advances in Genomics of Human Fungal Pathogens -- Chapter9. Yeast population genomics goes wild: The case of Saccharomyces paradoxus -- Part4. Population genomics of viruses -- Chapter10. Population genomics of plant viruses -- Chapter11. Population genomics of human viruses -- Chapter12. Population genomics of bacteriophages
    Abstract: Population genomics is a rapidly emerging field that has the potential to transform our understanding of how evolutionary forces shape genomic diversity among microbes. There have already been considerable advances in understanding gene flow and spread of adaptive traits, and in linking epidemiology with evolutionary biology. The current challenge is to find unifying evolutionary principles for organisms that display a wide range of reproductive biology – from highly clonal to promiscuous – and for which the vast majority have eluded cultivation. This requires interdisciplinary approaches that incorporate novel computational tools, testing of existing and novel population genetic models, and creative new ways of linking genetic diversity to ecological factors. This pioneering book will discuss the advances made and promises of population genomics in microorganisms, outlining some of the key theoretical and practical challenges for microbial population genomics, including defining and identifying populations, genomics-based reverse ecology and building appropriate tools to understand microbes in a variety of complex environments
    Pages: XIII, 344 p. : online resource.
    ISBN: 9783030047566
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  • 9
    Keywords: Plant genetics ; Ecology ; Plant breeding ; Plant anatomy ; Plant Ecology ; Medicine ; Plant Genetics and Genomics ; Community & Population Ecology ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Plant Ecology ; Health Promotion and Disease Prevention ; Springer eBooks
    Description / Table of Contents: Part1. Introduction -- Chapter1. Population genomics: Advancing understanding of nature -- Part2. Methods -- Chapter2. Genotyping and sequencing technologies in population genetics and genomics -- Chapter3. Computational tools for population genomics -- Chapter4. Population and evolutionary genetic inferences in the whole genome era: Software challenges -- Part3. Concepts and Approaches -- Chapter5. Population epigenomics: Advancing understanding of phenotypic plasticity, acclimation, adaptation and diseases -- Chapter6. Landscape genomics: Understanding heterogeneity and genomic characteristics of populations -- Chapter7. Paleogenomics: Genome-scale analysis of ancient DNA and population and evolutionary genomic inferences -- Chapter8. Genome-wide association studies and heritability estimation in the functional genomics era -- Chapter9. Genomic selection -- Part4. -- Chapter10. Population genomics provides key insights in ecology and evolution -- Chapter11. Inferring demographic history using genomic data -- Chapter12. Advancing biogeography through population genomics -- Chapter13. Adaptation without boundaries: Population genomics in ocean systems -- Chapter14. Population genomics of speciation and admixture -- Chapter15. Population genomics of colonization and invasion -- Chapter16. Population genomics of crop domestication: current state and perspectives -- Chapter17. Population genomics of animal domestication and breed development -- Chapter18. Population genomics of domestication and breed development in canines in the context of cognitive, social, behavioral, and disease traits
    Abstract: Population genomics has revolutionized various disciplines of biology including population, evolutionary, ecological and conservation genetics, plant and animal breeding, human health, medicine and pharmacology by allowing to address novel and long-standing questions with unprecedented power and accuracy. It employs large-scale or genome-wide genetic information and bioinformatics to address various fundamental and applied aspects in biology and related disciplines, and provides a comprehensive genome-wide perspective and new insights that were not possible before. These advances have become possible due to the development of new and low-cost sequencing and genotyping technologies and novel statistical approaches and software, bioinformatics tools, and models. Population genomics is tremendously advancing our understanding the roles of evolutionary processes, such as mutation, genetic drift, gene flow, and natural selection, in shaping up genetic variation at individual loci and across the genome and populations; improving the assessment of population genetic parameters or processes such as adaptive evolution, effective population size, gene flow, admixture, inbreeding and outbreeding depression, demography, and biogeography; resolving evolutionary histories and phylogenetic relationships of extant, ancient and extinct species; understanding the genomic basis of fitness, adaptation, speciation, complex ecological and economically important traits, and disease and insect resistance; facilitating forensics, genetic medicine and pharmacology; delineating conservation genetic units; and understanding the genetic effects of resource management practices, and assisting conservation and sustainable management of genetic resources. This Population Genomics book discusses the concepts, approaches, applications and promises of population genomics in addressing most of the above fundamental and applied crucial aspects in a variety of organisms from microorganisms to humans. The book provides insights into a range of emerging population genomics topics including population epigenomics, landscape genomics, seascape genomics, paleogenomics, ecological and evolutionary genomics, biogeography, demography, speciation, admixture, colonization and invasion, genomic selection, and plant and animal domestication. This book fills a vacuum in the field and is expected to become a primary reference in Population Genomics world-wide
    Pages: XVII, 822 p. 110 illus., 85 illus. in color. : online resource.
    ISBN: 9783030045890
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  • 10
    Keywords: Bioinformatics ; Genetics ; Human Genetics ; Agriculture ; Bioinformatics ; Genetics and Genomics ; Human Genetics ; Agriculture ; Springer eBooks
    Description / Table of Contents: Introduction to biological data -- Computers and bioinformatics -- Sequence biological databases -- Biological databases -- Other biological databases -- Nucleic acid sequencing -- Tools and methods in analysis of simple sequences -- Tools and methods in analysis of complex sequences -- Structural bioinformatics -- Protein structure annotations -- Introduction to functional bioinformatics -- Biological networks – tools, methods and analysis -- Metabolomics -- Drug discovery: concepts and approaches -- Drug designing methods and analysis -- Molecular molecular docking -- Insilico pcr -- Modeling and optimization of molecular bio-systems to generate predictive models -- Index
    Abstract: Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it mostly focuses on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. Unlike other bioinformatics books which are mostly theoretical, this book provides practical examples for the readers on state-of-the-art open source tools to solve biological problems. Flow charts of experiments, graphical illustrations, and mock data are included for quick reference. Volume I is therefore an ideal companion for students and early stage professionals wishing to master this blooming field
    Pages: XIV, 405 p. 193 illus., 186 illus. in color. : online resource.
    ISBN: 9783030026349
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  • 11
    Keywords: Medical genetics ; Medicine ; Biochemistry ; Neurosciences ; Gene Function ; Molecular Medicine ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Chapter 1. Molecular and Functional Properties of Progranulin -- Chapter 2. Progranulin as a biomarker for neurodegenerative diseases -- Chapter 3. PGRN and FTLD -- Chapter 4. PGRN and neurodegenerative diseases other than FTLD -- Chapter 5. Progranulin Regulations of Lysosomal Homeostasis and its Involvement in Neurodegenerative Diseases -- Chapter 6. Molecular and Functional Properties of Progranulin -- Chapter 7. PGRN and neuroinflammation -- Chapter 8. Neural Stem/Progenitor Cells and Progranulin -- Chapter 9. Generation and phenotyping of progranulin-deficient mice -- Chapter 10. Pleiotropic protective effects of progranulin in the treatment of ischemic stroke -- Chapter 11. New therapeutic approaches against ocular diseases
    Abstract: This book presents the latest knowledge on the roles of progranulin (PGRN) in normal physiology and pathology and explores the emerging significance of PGRN as a therapeutic target and biomarker in various CNS disorders, including frontotemporal lobe degeneration and other neurodegenerative diseases. Following initial recognition of the importance of PGRN in sexual differentiation of the developing brain and adult neurogenesis, it was subsequently discovered that PGRN acts as a chaperone of lysosomal enzymes and plays a crucial role in maintaining cellular protein homeostasis. It has also been found that sex steroids modulate the expression of PGRN and its trophic effects in the developing CNS and that PGRN directly or indirectly influences neural stem and progenitor cells. Against this background, deeper understanding of the molecular and functional properties of PGRN would provide fresh impetus for the development of mechanism-based therapeutic approaches for multiple disorders. Medications targeting the recovery of lysosomal function appear to hold particular promise in patients with neurodegenerative diseases resulting from PGRN insufficiency. In examining multiple aspects of this fascinating field, the book will be of high value for researchers and graduate students
    Pages: VII, 183 p. 37 illus., 13 illus. in color. : online resource.
    ISBN: 9789811361869
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  • 12
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Left Ventricular Strain and Relaxation are Independently Associated with Renal Cortical Perfusion in Hypertensive Patients -- Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: a Bispectral Analysis -- Influence of Heart Rate, Age, and Gender on Heart Rate Variability in Adolescents and Young Adults -- Hand-Foot Syndrome and Progression-Free Survival in Patients Treated with Sunitinib for Metastatic Clear Cell Renal Cell Carcinoma -- Elastography in the Diagnosis of Pancreatic Malignancies -- Factors Affecting Health-Related Quality of Life in Liver Transplant Patients -- Phagocytosis and Autophagy in THP-1 cells Exposed to Urban Dust: Possible Role of LC3€“Ássociated Phagocytosis and Canonical Autophagy -- Gendeŕ€“Dependent Responses of Growth and Insuliń€“like Growth Factoŕ€“1 to Growth Hormone Therapy in Prepubertal Growth Hormone Deficient Children -- Vitamin D and Calcium Homeostasis in Infants with Urolithiasis -- Morphometric Analysis of the Lumbar Vertebrae Concerning the Optimal Screw Selection for Transpedicular Stabilization -- Stress and Dehumanizing Behaviors of Medical Staff toward Patients -- Biological and Social Determinants of Maximum Oxygen Uptake in Adult Men
    Abstract: This book focuses on issues in clinical practice and research that are of general interest. The articles primarily focus on understanding the pathogenic mechanisms of diseases, their prevention, and therapy. The topics addressed include cardiovascular regulation with regard to blood pressure and heart rate variability, and to coupling blood pressure changes with subarachnoid fluid oscillations. In addition, the book discusses recent advances in the diagnostics of and targeted molecular therapy for renal and pancreatic malignancies, growth disorders, vitamin D and calcium homeostasis in children in the context of neonatal urolithiasis, and neurosurgical interventions in multifarious age-related diseases of the vertebrae. Neuropsychological aspects of patients’ quality of life and of shaping medical staff’s attitude toward patients are also addressed. The respective articles are intended to build a bridge between basic and clinical research. Further, the book enhances the current body of knowledge on diagnostics and patient treatment and offers valuable new perspectives on practical clinical issues. As such, it offers a unique resource for clinicians, family physicians, medical scholars, and professionals engaged in patient management
    Pages: VI, 114 p. 20 illus. in color. : online resource.
    ISBN: 9783030129231
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  • 13
    Keywords: Lipids ; Cell Membranes ; Lipidology ; Membrane Biology ; Springer eBooks
    Description / Table of Contents: 1. Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-stimulated Live Cells -- 2. Monitoring non-vesicular transport of phosphatidylserine and phosphatidylinositol 4-phosphate in intact cells by BRET analysis -- 3. Development of non-specific BRET-based biosensors to monitor plasma membrane inositol lipids in living cells -- 4. Following anterograde transport of phosphatidylserine in yeast in real time -- 5. Imaging lipid metabolism at the Golgi complex -- 6. Advanced in vitro assay system to measure PS and PE transport at ER/mitochondria interface -- 7. Measurement of lipid transport in mitochondria by the MTL complex -- 8. Bi- and trifunctional lipids for visualization of sphingolipid dynamics within the cell -- 9. Indirect lipid transfer protein activity measurements using quantification of glycosphingolipid production -- 10. Measurement of intracellular sterol transport in yeast -- 11. Intracellular and plasma membrane cholesterol labeling and quantification using filipin and GFP-D4 -- 12. Monitoring and modulating intracellular cholesterol trafficking using ALOD4, a cholesterol-binding protein -- 13. Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts -- 14. Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity -- 15. In vitro assays to measure the membrane tethering and lipid transport activities of the Extended Synaptotagmins -- 16. Determining the lipid binding specificity of SMP domains: an ERMES subunit as a case study -- 17. In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members -- 18. Purification and Characterization of Human Niemann–Pick C1 Protein -- 19. In vitro strategy to measure sterol/phosphatidylinositol 4-phosphate exchange between membranes -- 20. Determination of Ligand Binding Affinity and Specificity of Purified START Domains by Thermal Shift Assays Using Circular Dichoism -- 21. Synthesis of Fluorescent Membrane-spanning Lipids for Studies of Lipid Transfer and Membrane-Fusion -- 22. Setting-up all-atom molecular dynamics simulations to study the interactions of peripheral membrane proteins with model lipid bilayers
    Abstract: This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Intracellular Lipid Transport: Methods and Protocols serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process
    Pages: XI, 345 p. 70 illus., 56 illus. in color. : online resource.
    ISBN: 9781493991365
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  • 14
    Keywords: Bioinformatics ; Cytology ; Bioinformatics ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Quality Control of Single-cell RNA-seq -- Normalization for Single-cell RNA-seq Data Analysis -- Analysis of Technical and Biological Variability in Single-cell RNA Sequencing -- Identification of Cell Types from Single-cell Transcriptomic Data -- Rare Cell Type Detection -- scMCA- A Tool Defines Cell Types in Mouse Based on Single-cell Digital Expression -- Differential Pathway Analysis -- Differential Pathway Analysis -- Estimating Differentiation Potency of Single Cells using Single Cell Entropy (SCENT) -- Inference of Gene Co-expression Networks from Single-Cell RNA-sequencing Data -- Single-cell Allele-specific Gene Expression Analysis -- Using BRIE to Detect and Analyse Splicing Isoforms in scRNA-seq Data -- Preprocessing and Computational Analysis of Single-cell Epigenomic Datasets -- Experimental and Computational Approaches for Single-cell Enhancer Perturbation Assay -- Antigen Receptor Sequence Reconstruction and Clonality Inference from scRNA-seq Data -- A Hidden Markov Random Field Model for Detecting Domain Organizations from Spatial Transcriptomic Data
    Abstract: This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis
    Pages: X, 271 p. 168 illus., 156 illus. in color. : online resource.
    ISBN: 9781493990573
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  • 15
    Keywords: Embryology ; Laboratory animals ; Animal genetics ; Embryology ; Animal Models ; Animal Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments -- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos -- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos -- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts -- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis -- Embryological and Genetic Manipulation of Chick Development -- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos -- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens -- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) -- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos -- Ex Utero Culture and Imaging of Mouse Embryos -- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections -- Reptile Embryology and Regeneration -- Manipulation of Developmental Function in Turtles with Notes on Alligators -- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes -- Visualizing the Balbiani Body in Zebrafish Oocytes -- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis -- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos -- Germ Cell Transplantation in Avian Species -- Production of Germ-Line Chimeras in Zebrafish -- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring -- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) -- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish -- Modeling Embryonic Cleavage Patterns -- Laboratory Guidelines for Animal Care
    Abstract: This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development. Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via
    Pages: XI, 434 p. 82 illus., 70 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990092
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  • 16
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 17
    Keywords: Proteomics ; Biochemistry ; Proteomics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Pre- and Post-Analytical Factors in Biomarker Discovery -- Pre-Fractionation of Non-Circulating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers -- Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers -- Protein Biomarker Discovery Using Human Blood Plasma Microparticles -- A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry -- Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery -- Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies -- Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics -- Protein Biomarker Discovery in Non-Depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry -- Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry -- Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid -- Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions -- Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues -- Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications -- Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR -- Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis using CombiROC -- PanelomiX for the Combination of Biomarkers -- Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework
    Abstract: This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 292 p. 52 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991648
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  • 18
    Keywords: Medical parasitology ; Emerging infectious diseases ; Microbial genetics ; Microbial genomics ; Parasitology ; Infectious Diseases ; Microbial Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome -- Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development -- Transcriptome Studies in Trypanosoma cruzi using RNAseq -- An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene -- Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi -- Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes -- Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi -- Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae -- Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi -- Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase -- Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions -- Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues -- In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters -- Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models -- MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice -- Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR -- Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR -- Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase -- Analytics of Functional Autoantibodies in Patients with Chagas’ Disease -- Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease -- Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma -- Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease -- Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients -- Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients -- Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease -- CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease -- Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease -- Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts
    Abstract: This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information
    Pages: XVII, 398 p. 60 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991488
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  • 19
    Keywords: Cell receptors ; Lipids ; Receptors ; Lipidology ; Springer eBooks
    Description / Table of Contents: Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry
    Abstract: This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond
    Pages: XI, 220 p. 35 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991303
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  • 20
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: An Overview of CE in Clinical Analysis -- Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies -- Monitorization of α1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF Detection -- Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic Injection -- On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin -- Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis -- The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection -- Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization -- A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of Experiment -- New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS) -- Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Sample -- Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis -- Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis -- Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions -- A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood -- Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA) -- Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis -- Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen -- Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins
    Abstract: This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research
    Pages: XII, 273 p. 74 illus., 51 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992133
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  • 21
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 22
    Keywords: Pharmaceutical technology ; Biomedical Engineering ; Pharmaceutical Sciences/Technology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Molecular Biotechnology -- Biophysical and biochemical analysis of recombinant proteins -- Production and downstream processing of biotech products -- Formulation of biotech products, including biopharmaceutical considerations -- Pharmacokinetics and pharmacodynamics of peptide and protein drugs -- Immunogenicity of pharmaceutical proteins -- General considerations of monoclonal antibodies from structure to therapeutic application -- Genomics, Other €œÓmicś€ Technologies, Personalized Medicine and Additional Biotechnology-Related Techniques -- Dispensing biotechnology products: handling, professional education and product information -- Economic considerations in medical biotechnology -- Regulatory framework for biosimilars -- Vaccines -- Oligonucleotides and siRNA -- Gene therapy -- Stem cell technology -- Therapeutic Applications.-Endocrinology -- Insulin -- Follicle-stimulating hormone -- Growth hormone -- Cardiovascular and Respiratory Applications -- Recombinant coagulation factors and thrombolytics -- Recombinant human deoxyribonuclease -- Oncology -- Monoclonal antibodies in oncology -- Hematopoetic growth factors -- Inflammation and Immunemodulation -- Monoclonal antibodies in transplantation -- Monoclonal antibodies and antibody-based therapeutics in anti-inflammatory therapy -- Interferons and interleukins -- Anti-infectious diseases -- Monoclonal antibodies -- Enzyme replacement therapy
    Abstract: This introductory text explains both the basic science and the applications of biotechnology derived pharmaceuticals, with special emphasis on their clinical use. It serves as a complete one-stop source for undergraduate/graduate pharmacists, pharmaceutical science students, and for those who did not receive formal training in this field. Pharmaceutical Biotechnology: Fundamentals and Applications, Fifth Edition completely updates the previous edition, and includes additional coverage on newer approaches such as oligonucleotides, mi/siRNA, gene therapy and advanced therapeutics. Daan J.A. Crommelin is Professor Emeritus Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences at Utrecht University, Utrecht, The Netherlands and Adjunct Professor in the Department of Pharmaceutics and Pharmaceutical Chemistry at the University of Utah, Salt Lake City, UT, USA Robert D. Sindelar is Professor and former Dean Faculty of Pharmaceutical Sciences and The Centre for Health Evaluation & Outcomes Sciences (CHEOS), The University of British Columbia (UBC), Vancouver, BC, Canada and President of the Global Drug Commercialization Centre (GDCC)-China and Vice-President GDCC-worldwide, Chengdu, China Bernd Meibohm is Professor of Pharmaceutical Sciences and Associate Dean for Research and Graduate Programs at the University of Tennessee Health Science Center, College of Pharmacy, Memphis, TN, USA
    Pages: XXIII, 653 p. 246 illus., 227 illus. in color. : online resource.
    Edition: 5th ed. 2019.
    ISBN: 9783030007102
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  • 23
    Keywords: Human Genetics ; Bioinformatics ; Human Genetics ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Chapter1. Strategies for converting RNA to amplifiable cDNA for single-cell RNA sequencing methods -- Chapter2. Integrated Fluidic Circuits for single cell omics and multi-omics applications -- Chapter3. Single-cell DNA-Seq and RNA-Seq in cancer using the C1 system -- Chapter4. Nx1-Seq (Well based Single-Cell Analysis System) -- Chapter5. Quantitation of mRNA transcripts and proteins using the BD Rhapsody™ Single-Cell Analysis System -- Chapter6. An Informative Approach to Single-Cell Sequencing Analysis -- Chapter7. Bionano Genome Mapping: High-Throughput, Ultra-Long Molecule Genome Analysis System for Precision Genome Assembly and Haploid-Resolved Structural Variation Discovery -- Chapter8. Informatics for PacBio long reads -- Chapter10. On-site MinION sequencing
    Abstract: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians
    Pages: VI, 150 p. 56 illus., 51 illus. in color. : online resource.
    ISBN: 9789811360374
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  • 24
    Keywords: Biochemistry ; Cytology / Research ; Cytology ; Genetics ; Biochemistry, general ; Biological Techniques ; Medical Biochemistry ; Cell Biology ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Ionic and Covalent Bonds -- Introduction to Biological Macromolecules -- Carbohydrates -- Enzyme Kinetics
    Abstract: This textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The “Labster Virtual Lab Experiments” series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn’t have access to. In this book, you’ll learn the fundamental concepts of basic biochemistry focusing on: Ionic and Covalent Bonds Introduction to Biological Macromolecules Carbohydrates Enzyme Kinetics In each chapter, you’ll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you’ll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access to five simulations for the duration of six months. If you’re using the e-book version, you can sign up and buy access to the simulations at If you like this book, try out other topics in this series, including “Basic Biology”, “Basic Genetics”, and “Genetics of Human Diseases”. About Labster Labster is a company dedicated to developing virtual lab simulations that are designed to stimulate students’ natural curiosity and highlight the connection between science and the real world. These simulations have been shown to improve the achievement of learning outcomes among students, by making the learning experience more immersive and engaging. The content of this book was created by the Labster team members Dr. Aaron Gardner, Dr. Wilko Duprez, Dewi Ayu Kencana Ungu, Dr. Sarah Stauffer, Dr. Frederik Clauson-Kaas, and Silvia Tjong. You can learn more about Labster and explore the simulations at
    Pages: IX, 87 p. 47 illus. : online resource.
    ISBN: 9783662584996
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  • 25
    Keywords: Proteomics ; Medical genetics ; Diabetes ; Proteomics ; Gene Function ; Diabetes ; Springer eBooks
    Description / Table of Contents: 1. Insulin Resistance in Schizophrenia -- 2. Biogenesis of the Insulin Secretory Granule in Health and Disease -- 3. Current Models of Fatty Liver Disease; New Insights, Therapeutic Targets and Interventions -- 4. Preclinical Models of Altered Early Life Nutrition and Development of Reproductive Disorders in Female Offspring -- 5. Targeting Mitochondrial Defects to Increase Lngevity in Animal Models of Neurodegenerative Diseases -- 6. Computational Approaches for Identification of Pleiotropic Biomarker Profiles in Psychiatry -- 7. MiRNA Regulation of Glucose and Lipid Metabolism in Relation to Diabetes and Non-Alcoholic Fatty Liver Disease -- 8. Hypercaloric Diet-Induced Obesity and Obesity-Related Metabolic Disorders in Experimental Models -- 9. Metabolic Biomarkers in Nematode C. elegans During Aging -- 10. Circular RNAs as potential biomarkers and therapeutic targets for metabolic diseases -- 11. Deciphering Endothelial Dysfunction in the HIV-Infected Population -- 12. The Role of Inflammation in the Development of GDM and The Use of Markers of Inflammation in GDM Screening -- 13. PFKP Signaling at a Glance: an Emerging Mediator of Cancer Cell Metabolism -- 14. ATAD3A on the Path to Cancer -- 15. Impact of Exercise on Inflammatory Mediators of Metabolic and Vascular Insulin Resistance in Type 2 Diabetes
    Abstract: The book recognizes that throughout the scientific, medical, and economic communities, new tests incorporating biomarkers are needed to improve the diagnosis of patients suffering from metabolic disorders. The early identification of those at risk of developing obesity will help to place these individuals on the best treatment course as early as possible for improved treatment outcomes. This will also help to cut costs incurred by the healthcare services. For all of this to occur, new research efforts are needed to identify novel biomarkers that can be used to predict the disease in the presymptomatic stage, for disease monitoring and for prediction of treatment response. It is also possible that new drug targets can be identified using these approaches which, in turn, can lead to the development of new treatment approaches. This volume also includes a series of reviews on biomarker discovery and usage in the study of diseases marked by perturbations in metabolism. It will describe the pros and cons of the various approaches and cover the successes and failures in this important research field
    Pages: XI, 303 p. 39 illus., 22 illus. in color. : online resource.
    ISBN: 9783030126681
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  • 26
    Keywords: Biomedical Engineering ; Cytology / Research ; Microbial ecology ; Microbiology ; Plant Diseases ; Biomedical Engineering/Biotechnology ; Biological Techniques ; Microbial ecology ; Microbiology ; Plant Pathology ; Springer eBooks
    Description / Table of Contents: Part I: Root symbioses -- Chapter 1. Synthetic plasmids to challenge symbiotic nitrogen fixation between rhizobia and legumes -- Chapter 2. In vivo analysis of rhizosphere enzyme activities by the use of plastic syringes -- Chapter 3. Characterization of arbuscular mycorrhizal communities in roots of vineyard plants -- Chapter 4. Molecular methods for research on actinorhiza -- Chapter 5. Molecular and functional characterization of beneficial bacteria associated with AMF spores -- Part II: Plant pathogens and microbial plant protection -- Chapter 6. Oomycete-root interactions -- Chapter 7. Exploitation of rhizosphere microbiome services -- Chapter 8. Methods for detecting biocontrol and plant growth-promoting traits in rhizobacteria -- Chapter 9. A split-root method to study systemic and heritable traits induced by Trichoderma in tomato plants -- Part III: Experimental approaches and analytical techniques in rhizosphere biology -- Chapter 10. Using a tri-isotope (13C, 15N, 33P) labelling method to quantify rhizodeposition -- Chapter 11. Microscopic techniques coupled to molecular and genetic approaches to highlight cell-type specific differences in mycorrhizal symbiosis -- Chapter 12. From imaging to functional traits in interactions between roots and microbes -- Chapter 13. Live imaging of arbuscular mycorrhizal symbiosis -- Chapter 14. Microcosm approaches to investigate multitrophic interactions in microbial communities -- Chapter 15. Analysis of common mycorrhizal networks in microcosms
    Abstract: This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation
    Pages: IX, 279 p. 37 illus., 34 illus. in color. : online resource.
    ISBN: 9789811357671
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  • 27
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK! -- Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis -- Identification of Long Non-Coding RNAs in the Developing Endosperm of Maize -- RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize -- Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues -- Medium Throughput RNA In Situ Hybridization of Serial Sections from Paraffin-Embedded Tissue Microarrays -- Purification and Functional Analysis of Plant Long Non-Coding RNAs (lncRNA) -- The Involvement of Long Non-Coding RNAs in Response to Plant Stress -- Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance -- Discovery, Identification, and Functional Characterization of Plant Long Intergenic Non-Coding RNAs after Virus Infection -- Bioinformatics Approaches to Studying Plant Long Non-Coding RNAs (lncRNAs): Identification and Functional Interpretation of lncRNAs from RNA-seq Data Sets -- Identification of Novel lincRNA and Co-Expression Network Analysis using RNA Sequencing Data in Plants -- An Easy-to-Follow Pipeline for Long Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria vesca -- Reference-Based Identification of Long Non-Coding RNAs in Plants with Strand-Specific RNA-Sequencing Data -- NAMS: Non-Coding Assessment in Magnoliophyta Species -- De novo Plant Transcriptome Assembly and Annotation using Illumina RNA-seq Reads -- Identification of Long Non-Coding RNA-Protein Interactions through In Vitro RNA Pull-Down Assay with Plant Nuclear Extracts -- Analysis of Interaction between Long Non-Coding RNAs and Protein by RNA-Immunoprecipitation in Arabidopsis -- Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants -- In Vivo Genome-Wide RNA Structure Probing with Structure-seq2 -- Using Protein Interaction Profile Sequencing (PIP-seq) to Identify RNA Secondary Structure and RNA-Protein Interaction Sites of Long Non-Coding RNAs in Plants -- Computationally Characterizing Protein Bound Long Non-Coding RNAs and their Secondary Structure using Protein Interaction Profile Sequencing (PIP-seq) in Plants -- Stalking Structure in Plant Long Non-Coding RNAs -- Transcriptome-Wide Mapping 5-methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant -- A Walkthrough to the Use of GreeNC: The Plant lncRNA Database -- CANTATAdb 2.0: Expanding the Collection of Plant Long Non-Coding RNAs -- Experimentally Validated Plant lncRNAs in EVLncRNAs Database -- In Situ Hi-C for Plants: An Improved Method to Detect Long-Range Chromatin Interactions
    Abstract: This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology
    Pages: XVII, 480 p. 78 illus., 67 illus. in color. : online resource.
    ISBN: 9781493990450
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  • 28
    Keywords: Biochemistry ; Cytology ; Cell receptors ; Protein Science ; Cell Biology ; Receptors ; Springer eBooks
    Description / Table of Contents: A Brief History of the b-Arrestins -- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling -- Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET -- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle -- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer -- Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET -- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin -- Methods to Investigate Arrestins in Complex with Phosphodiesterases -- Methods to Characterize Protein Interactions with β-arrestin in cellulo -- Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling -- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing -- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners -- Workflow Description to Dynamically Model β-Arrestin Signaling Networks -- Proteomic Analysis of the β-Arrestin Interactome -- Quantitating Ligand Bias using the Competitive Model of Ligand Activity -- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins -- Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy -- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins -- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors -- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling -- Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease -- Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function -- Methods to Investigate β-Arrestin Function in Metabolic Regulation -- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease -- Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells
    Abstract: This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins
    Pages: XIV, 409 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493991587
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  • 29
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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  • 30
    Keywords: Immunology ; Food science ; Immunology ; Food science ; Public Health ; Springer eBooks
    Description / Table of Contents: Overview of food allergy and gut mucosal immunity -- Species and structure of food allergens: epitopes and cross-reactivity -- Detection and quantification methods for food allergens -- Allergenicity evaluation of food proteins -- Foodprocessing to eliminate food allergens and development of hypoallergenic foods -- Modulation of food allergy by bioactive natural compounds and development of functional foods -- Food allergy and the microbiota: implications for probiotic use in regulating allergic responses -- Intestinal permeability and transport of food allergens -- Risk assessment and control management of food allergens
    Abstract: This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields
    Pages: XIII, 216 p. 32 illus., 25 illus. in color. : online resource.
    ISBN: 9789811369285
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  • 31
    Keywords: Human Physiology ; Neurosciences ; Cytology ; Radiology, Medical ; Stem Cells ; Nanotechnology ; Human Physiology ; Neurosciences ; Oxidative Stress ; Diagnostic Radiology ; Stem Cells ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Chapter 1. Cerebral stroke: An Introduction -- Chapter 2. Inflammation, oxidative stress, and neurodegeneration -- Chapter 3. Stroke induced blood brain barrier damage -- Chapter 4. Regulation of calcium ions in ischemic neuronal cell -- Chapter 5. Ischemic stroke induced endoplasmic reticulum stress -- Chapter 6. The role of autophagy in ischemic stroke: friend or foe?- Chapter 7. Critical role of mitochondrial autophagy in cerebral stroke -- Chapter 8. Application of neuroimaging in the identification of the pinpoint location of blockage -- Chapter 9. Emerging role of the electromagnetic field in stroke -- Chapter 10. Stem cell therapies for stroke -- Chapter 11. MicroRNA: Significance to stroke diagnosis, prognosis, and therapy -- Chapter 12. Therapeutic Aspects of Nanomedicines in Stroke Treatment -- Chapter 13. Neuroprotective potential of small molecule phytochemicals against stroke -- Chapter 14. Role of UV irradiation on neuroprotective potential of phytochemicals -- Chapter 15. Post-stroke treatment strategies, management, and rehabilitation
    Abstract: This book provides detailed and comprehensive mechanistic insights of the various risk factors that lead to the ischemic stroke and the novel therapeutic interventions against it. The first section discusses the different ischemic cerebral stroke-induced inflammatory pathways and dysfunctionality of blood-brain barrier. The later sections of the book deals with the role of endoplasmic reticulum stress and mitophagy in cerebral stroke and introduces the different neuroimaging techniques such as Computed tomography (CT), Magnetic resonance imaging (MRI), Positron emission tomography (PET) and Single-Photon emission computed tomography (SPECT) that are used to identify the arterial blockages. The final section comprises of chapters that focus on various neuroprotective strategies and emerging therapeutic interventions for combating stroke pathophysiology. The chapters cover the role of stem cell therapy, the therapeutic effect of low-frequency electromagnetic radiations (LF-EMR), and implications of non-coding RNAs such as micro-RNAs as the biomarkers for diagnosis, prognosis, and therapy in ischemic stroke
    Pages: VIII, 189 p. 23 illus., 12 illus. in color. : online resource.
    ISBN: 9789811314537
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  • 32
    Keywords: Toxicology ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Complexity of Biologics CMC Regulation -- Biopharmaceutics are Not Chemical Drugs -- An Effective CMC Strategy is Possible -- Challenge of Adventitious Agent Control -- Biopharmaceutical Source Materials -- Manufacturing of Biopharmaceutical APIs -- Manufacturing of the Drug Product -- Complex Process-Related Impurity Profiles -- Product Characterization is a Journey -- Priceless Potency (Therapeutic Activity) -- Quality Attributes of a Biopharmaceutical -- Designing the Stability Program -- The Art of Setting Specifications -- Demonstrating Product Comparability After Process Changes -- Invaluable CMC-Focused Meetings with Regulatory Authorities
    Abstract: This book since first published in 2004 has been a major resource providing insights and practical guidance for the CMC teams to develop an acceptable cost-effective, risk-based CMC regulatory compliance strategy for biopharmaceuticals (recombinant proteins, monoclonal antibodies, genetically engineered viruses and genetically engineered human cells) from early clinical stage development through market approval. The third edition of this book provides added coverage for the biosimilars, antibody drug conjugates (ADCs), bispecific antibodies, genetically engineered viruses, and genetically engineered cells. This third edition of the book also addresses the heightened pressure on CMC regulatory compliance timelines due to the introduction of expedited clinical pathways moving the clinical development closer to a seamless phase process (e.g., FDA Breakthrough Therapy designation, CBER Regenerative Medicine Advanced Therapy (RMAT) designation, EMA Priority Medicines (PRIME) designation). The Challenge of CMC Regulatory Compliance for Biopharmaceuticals is essential, practical information for all pharmaceutical development scientists, Manufacturing and Quality Unit staff, Regulatory Affairs personnel, and senior management involved in the manufacture of biopharmaceuticals
    Pages: XXVIII, 426 p. 59 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9783030137540
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  • 33
    Keywords: Microbiology ; Medicine ; Emerging infectious diseases ; Medical Microbiology ; Health Promotion and Disease Prevention ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Diagnostics, Therapeutics and Other Potential Strategies of Biodefense -- Advanced Molecular Biology Tools & Diagnostics -- High Containment Infectious Disease Laboratories as an Integral Part of Emergency Preparedness -- Decontamination Capabilities and Facilities -- Virogenomics as a Tool for Molecular Epidemiology and Forensics -- Next Generation Sequencing Tools for Pathogen Detection -- Metagenomics -- Point-of-care Diagnostic Facilities for Outbreak Intervention -- Psychological Impact of Biological Attacks -- Public-health Preparedness -- Viral Therapeutics -- Bacterial Therapeutics -- Emergency Training for Veterinary Professionals -- Global Biodefense Coordination
    Abstract: This second volume of a two-volume set focuses on specific pathogens and their mechanisms of pathogenesis as well as diagnostics, therapeutics and various strategies in the event of biological attacks. This multidisciplinary book appeals to readers from various fields, including biodefense, biosafety & biosecurity, virology, neurology, molecular biology and genetic engineering, as well as infectious disease specialists. Further, the work is of interest to basic science and applied science research scholars and experts working in the area of high-consequence or select agent virology
    Pages: XIII, 383 p. 30 illus., 25 illus. in color. : online resource.
    ISBN: 9783030030711
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  • 34
    Singapore : Springer
    Keywords: Bioinformatics ; Medicine ; Statistics ; Bioinformatics ; Biomedicine general ; Statistics for Life Sciences, Medicine, Health Sciences ; Springer eBooks
    Description / Table of Contents: Part 1. BIOINFORMATICS FOR LIFE AND PERSONAL GENOME INTERPRETATION -- Chapter 1. Bioinformatics For Life -- Chapter 2. Next Generation Sequencing and Personal Genome Data Analysis -- Chapter 3. Personal Genome Data Analysis -- Chapter 4. Personal Genome Interpretation and Disease Risk Prediction -- Part 2. ADVANCED MICROARRAY DATA ANALYSIS -- Chapter 5. Advanced Microarray Data Analysis -- Chapter 6. Gene Expression Data Analysis -- Chapter 7. Gene Ontology and Biological Pathway-based Analysis -- Chapter 8. Gene-set Approaches and Prognostic Subgroup Prediction -- Chapter 9. MicroRNA Data Analysis -- Part 3. NETWORK BIOLOGY, SEQUENCE, PATHWAY AND ONTOLOGY INFORMATICS -- Chapter 10. Network Biology, Sequence, Pathway and Ontology Informatics -- Chapter 11. Motif and Regulatory Sequence Analysis -- Chapter 12. Molecular Pathways and Gene Ontology -- Chapter 13. Biological Network Analysis -- Part 4. SNPS, GWAS AND CNVS, INFORMATICS FOR GENOME VARIANTS -- Chapter 14. SNPs, GWAS, CNVs: Informatics for Human Genome Variations -- Chapter 15. SNP Data Analysis -- Chapter 16. GWAS Data Analysis -- Chapter 17. CNV Data Analysis -- Part 5. METAGENOME AND EPIGENOME, BASIC DATA ANALYSIS -- Chapter 18. Metagenome and Epigenome Data Analysis -- Chapter 19. Metagenome Data Analysis -- Chapter 20. Epigenome Databases and Tools -- Chapter 21. Epigenome Data Analysis -- Appendix A. BASIC PRACTICE USING R FOR DATA ANALYSIS -- Appendix B. APPLICATION PROGRAM FOR GENOME DATA ANALYSIS INSTALL GUIDE
    Abstract: This textbook describes recent advances in genomics and bioinformatics and provides numerous examples of genome data analysis that illustrate its relevance to real world problems and will improve the reader’s bioinformatics skills. Basic data preprocessing with normalization and filtering, primary pattern analysis, and machine learning algorithms using R and Python are demonstrated for gene-expression microarrays, genotyping microarrays, next-generation sequencing data, epigenomic data, and biological network and semantic analyses. In addition, detailed attention is devoted to integrative genomic data analysis, including multivariate data projection, gene-metabolic pathway mapping, automated biomolecular annotation, text mining of factual and literature databases, and integrated management of biomolecular databases. This textbook is primarily intended for life scientists, medical scientists, statisticians, data processing researchers, engineers, and other beginners in bioinformatics who are experiencing difficulty in approaching the field. However, it will also serve as a simple guideline for experts unfamiliar with the new, developing subfield of genomic analysis within bioinformatics
    Pages: XVI, 367 p. 645 illus., 236 illus. in color. : online resource.
    ISBN: 9789811319426
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  • 35
    Keywords: Pharmaceutical technology ; Analytical biochemistry ; Biochemistry ; Pharmaceutical Sciences/Technology ; Analytical Chemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recognition Mechanisms of Chiral Selectors: An Overview -- Enantioseparation by Thin Layer Chromatography -- Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase -- Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS -- Chiral Mobile Phase Additives in HPLC Enantioseparations -- Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High Performance Liquid Chromatography: An Overview -- HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions -- Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC -- HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases -- Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector -- Cyclofructans as Chiral Selectors: An Overview -- High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview -- Application of Sub-2 Micron Particles Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography -- Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview -- Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand-Exchange -- Applications of Chiral Supercritical Fluid Chromatography -- Chiral Separations by Countercurrent Chromatography -- Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations -- Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations -- Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer -- Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins -- Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes -- Chiral Capillary Electrophoresis-Mass Spectrometry -- Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography -- Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry -- Application of a (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography -- Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview
    Abstract: This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry
    Pages: XIV, 487 p. 147 illus., 57 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493994380
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  • 36
    Keywords: Stem Cells ; Biology / Data processing ; Bioinformatics ; Stem Cells ; Computer Appl. in Life Sciences ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: Agent-Based Modeling to Delineate Spatiotemporal Control Mechanisms of the Stem Cell Niche -- Modeling Cellular Differentiation and Reprogramming with Gene Regulatory Networks -- Cell Population Model to Track Stochastic Cellular Decision-Making during Differentiation -- Automated Formal Reasoning to Uncover Molecular Programs of Self-Renewal -- Mathematical Modeling of Clonal Stem Cell Dynamics -- Computational Tools for Quantifying Concordance in Single Cell Fate -- Quantitative Modeling of the Waddington Epigenetic Landscape -- Modeling Gene Networks to Understand Multistability in Stem Cells -- Trajectory Algorithms to Infer Stem Cell Fate Decisions -- Gene Regulatory Networks from Single Cell Data for Exploring Cell Fate Decisions -- Reconstructing Gene Regulatory Networks that Control Hematopoietic Commitment -- Investigating Cell Fate Decisions with ICGS Analysis of Single Cells -- Lineage Inference and Stem Cell Identity Prediction using Single Cell RNA-Sequencing Data -- Dynamic Network Modeling of Stem Cell Metabolism -- Metabolomics in Stem Cell Biology Research -- Molecular Interaction Networks to Select Factors for Cell Conversion -- A Computational Approach for Cell Fate Reprogramming -- Computational Analysis of Aneuploidy in Pluripotent Stem Cells -- Cell Fate Engineering Tools for iPSC Disease Modeling
    Abstract: This volume details methods and protocols to further the study of stem cells within the computational stem cell biology (CSCB) field. Chapters are divided into four sections covering the theory and practice of modeling of stem cell behavior, analyzing single cell genome-scale measurements, reconstructing gene regulatory networks, and metabolomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Stem Cell Biology: Methods and Protocols will be an invaluable guide to researchers as they explore stem cells from the perspective of computational biology
    Pages: XI, 456 p. 107 illus., 95 illus. in color. : online resource.
    ISBN: 9781493992249
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  • 37
    Keywords: Biochemistry ; Pharmaceutical technology ; Biochemistry, general ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels -- Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6 -- Cryo-Electron Microscopy of TRP Channels -- Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation -- Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels -- Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique -- Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels -- High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons -- Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels -- TRP Channels Reconstitution in Lipid Bilayers -- Methods for Investigating TRP Channel Gating -- In Silico Approaches for TRP Channel Modulation -- Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction -- In Vivo Methods to Study ThermoTRP Channels in Rodents
    Abstract: This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels
    Pages: X, 238 p. 69 illus., 58 illus. in color. : online resource.
    ISBN: 9781493994465
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  • 38
    Keywords: Nucleic Acids ; Genetics ; Nucleic Acid Chemistry ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs -- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine -- Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue -- Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA -- 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability -- Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids -- Synthesis of Nucleobase-Functionalized Morpholino Monomers -- Synthesis and Application of LKγT Peptide Nucleic Acids -- Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids -- Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site -- Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids -- Labeling Peptide Nucleic Acids with Indium-111 -- Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet -- Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates -- Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis -- FRET Assay for Ligands Targeting the Bacterial A-Site RNA -- The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons -- Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA -- Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation
    Abstract: This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors
    Pages: XI, 315 p. 95 illus., 41 illus. in color. : online resource.
    ISBN: 9781493992164
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  • 39
    Keywords: Developmental Biology ; Cytology ; Developmental Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The neural crest: a remarkable model system for studying development and disease -- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example -- Generation of neural crest progenitors from human pluripotent stem cells -- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling -- Deciphering the neural crest contribution to cephalic development with avian embryos -- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis -- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants -- Technologies for live imaging of enteric neural crest derived cells -- Mouse embryo culture for the study of neural crest cells -- Laser capture microdissection of murine embryonic neural crest cells -- In vivo and in vitro quantitative analysis of neural crest cell migration -- Proteomics in neural crest cell research -- Neural crest methodologies in zebrafish and medaka -- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells -- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity -- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective
    Abstract: This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike
    Pages: X, 230 p. 36 illus. in color. : online resource.
    ISBN: 9781493994120
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  • 40
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) -- Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells -- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD -- Chromatin Immunoprecipitation in Skeletal Myoblasts -- Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor -- Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation -- Lentivirus-Mediated RNAi in Skeletal Myogenesis -- Adipogenesis from Bovine Precursors -- Transcriptomic Profiling during Myogenesis -- Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle -- Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System -- LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue -- siRNA Gene Silencing during C2C12 Myogenesis -- Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury -- Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis -- Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis -- Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers -- Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis -- Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies -- RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis
    Abstract: This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis
    Pages: XII, 351 p. 84 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988976
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  • 41
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Phytoplasmas: An Introduction -- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques -- Phytoplasma Transmission: Insect Rearing and Infection Protocols -- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors -- Symptoms of Phytoplasma Diseases -- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas -- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene -- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas -- Real-time PCR Protocol for Phytoplasma Detection and Quantification -- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves -- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas -- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas -- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification -- Quantitative Analysis with Droplet Digital PCR -- Rapid Sample Preparation and LAMP for Phytoplasma Detection -- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly -- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’ -- Transcriptomic Analyses of Phytoplasmas -- Transcriptomic Analyses of Phytoplasmas -- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study -- Collection of Phloem Sap in Phytoplasma-infected Plants -- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas -- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas -- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) -- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants -- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants
    Abstract: This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 362 p. 75 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988372
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  • 42
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Protocol to Compare Methods for Untargeted Metabolomics -- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry -- Measuring the Nutrient Metabolism of Adherent Cells in Culture -- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells -- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice -- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro -- Measuring Rates of ATP Synthesis -- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay -- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers -- Determining Compartment Specific Metabolic Fluxes -- Determining the Impact of Metabolic Nutrients on Autophagy -- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism -- Determining Macrophage Polarization upon Metabolic Perturbation -- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells -- Development of Patient-derived Tumor Xenograft Models -- Imaging Glioma Progression by Intravital Microscopy -- Lipectomizing Mice for Applications in Metabolism -- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants -- Determining the Intracellular Organization of Organelles -- The Fundamentals of Constructing and Interpreting Heat Maps
    Abstract: This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level
    Pages: XII, 294 p. 67 illus., 53 illus. in color. : online resource.
    ISBN: 9781493987696
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  • 43
    Keywords: Oncology ; Neurosciences ; Cancer Research ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Introduction to Brain Tumor Stem Cells -- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells -- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines -- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors -- Detecting Stem Cell Marker Expression Using the NanoString nCounter System -- Flow Cytometric Analysis of Brain Tumor Stem Cells -- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells -- Differentiation of Brain Tumor Initiating Cells -- The Study of Brain Tumor Stem Cell Migration -- The Study of Brain Tumor Stem Cell Invasion -- Cell Cycle Dynamics in Glioma Cancer Stem Cells -- Embryonic Stem Cell Models of Human Brain Tumors -- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory -- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF -- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells -- In Vitro Assays for Screening Small Molecules -- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma -- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci -- In Vivo Murine Models of Brain Metastasis -- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain
    Abstract: This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer
    Pages: XII, 254 p. 40 illus., 34 illus. in color. : online resource.
    ISBN: 9781493988051
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  • 44
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinsoń€™s Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- ℗Ư℗ƯMonitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the €œáutophagic flux́€ in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations
    Abstract: This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XVII, 741 p. 137 illus., 99 illus. in color. : online resource.
    ISBN: 9781493988730
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  • 45
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility -- Isolation of Human Peripheral Blood T Lymphocytes -- A Laboratory Model to Study T-Cell Motility -- Quantification of T-Cell Migratory Phenotypes using High Content Analysis -- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time -- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells -- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis -- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes -- GapmeR-Mediated Gene Silencing in Motile T-Cells -- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy -- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes -- Immunometabolomic Phenotyping of Motile T-Cells -- Profiling Activity of Cellular Kinases in Migrating T-Cells -- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes -- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy -- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides -- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility -- Computational Analysis of Protein-Protein Interactions in Motile T-Cells
    Abstract: This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging
    Pages: X, 158 p. 31 illus., 28 illus. in color. : online resource.
    ISBN: 9781493990368
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  • 46
    New York, NY : Springer
    Keywords: Monoclonal antibodies ; Medical laboratories ; Antibodies ; Laboratory Medicine ; Springer eBooks
    Description / Table of Contents: Autoantibodies as Diagnostic Tools -- Non-specific Binding in Immunoassays for Autoantibodies -- Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells -- Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence -- Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry -- Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry -- Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry -- Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay -- Determination of Autoantibodies to Salivary Gland Antigens -- Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS) -- Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay -- Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay -- Determination of Autoantibodies to C3 Nephritic Factors by ELISA -- Determination of Anti-C1q Autoantibodies by Western Blot -- Anti-Factor H Autoantibodies Assay by ELISA -- Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay -- Determination of CRP Autoantibodies by SPR Immunoassay -- Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis -- Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay -- Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate -- The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay -- Determination of Autoantibodies by Line Blotting -- Determination of Rheumatoid Factors by ELISA -- Detection of autoantibodies to complement components by surface plasmon resonance-based technology
    Abstract: This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients
    Pages: XII, 289 p. 58 illus., 32 illus. in color. : online resource.
    ISBN: 9781493989492
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  • 47
    Keywords: Gene Therapy ; Oncology ; Medicine ; Gene Therapy ; Cancer Research ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Origins of Suicide Gene Therapy -- Cancer Suicide Gene Therapy with TK.007 -- Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer -- Suicide Gene Therapy for Oral Squamous Cell Carcinoma -- Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy -- Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer -- Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy -- Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells -- Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45α to Arrest the G2/M Transition -- Non-Invasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging -- In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector -- Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells -- Photochemical Internalization Enhanced Non-Viral Suicide Gene Therapy -- Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro
    Abstract: This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy
    Pages: XII, 190 p. 37 illus., 26 illus. in color. : online resource.
    ISBN: 9781493989225
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  • 48
    New York, NY : Springer
    Keywords: Neurosciences ; Neurosciences ; Springer eBooks
    Description / Table of Contents: An Overview of the Blood-Brain Barrier -- Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics -- Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modelling of the Blood-Brain Barrier -- In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems -- In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress in Brain Microvascular Endothelial Cell Phenotype -- Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier -- Cell Penetrating Peptides as Theranostics against Impaired Blood-Brain barrier Permeability: Implications for Pathogenesis and Therapeutics Treatment of Neurodegenerative Disease -- Microbial Translocation of the Blood-Brain Barrier -- Transport across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System -- Drosophila as a Model to Study the Blood-Brain Barrier -- Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier -- Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents -- Experimental Tools to Study the Regulation and Function of the Choroid Plexus -- Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid acorss the Blood-Cerebrospinal Fluid Barrier -- In Vivo Analysis to Study Transport across the Blood-Retinal Barrier -- Increasing BBB Permeability via Focused Ultrasound: Current Methods in Pre-Clinical Research -- Evaluation of Blood-Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evan’s Blue Extravasation -- Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy -- Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECT -- Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage Measurement
    Abstract: This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches
    Pages: XIII, 367 p. 77 illus., 54 illus. in color. : online resource.
    ISBN: 9781493989461
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  • 49
    Keywords: Neurosciences ; Biochemistry ; Neurosciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Co-Immunoprecipitation Methods for Detection of G Protein-Coupled Receptors in Brain Tissue -- Co-Immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaptic Density in Brain Tissue -- Use of Co-Immunoprecipitation to Study Conformation-Specific protein Interactions of Oligomeric α-Synuclein Aggregates -- Using Co-Immunoprecipitation and Shotgun Mass Spectrometry for protein-Protein Interaction Identification in Cultured Human Oligodencrocytes -- Co-Immunoprecipition Analysis of GPCR Complexes in the Central Nerve System -- The Histoblot Technique: A Reliable Approach to Analyse Expression Profile of proteins and to Predict their Molecular Association -- Co-Immunoprecipitation Assay to Investigate the Interaction Strength between Synaptic Protein using COS-7 Cells -- Guanosine-5’-O-(3-[35S]thio)triphosphate ([35S]GTPS) Binding/Immunoprecipitation Assay Using Magnetic Beads Coated with Anti-G Antibody in Mammalian Brain Membranes -- Co-Immunoprecipitation as a Useful Tool for Detection of G protein-Coupled Receptors Oligomers -- Isolation and Detection of G Protein–Coupled receptor (GPCR) Heteroreceptor Complexes in Rat Brain Synaptosomal Preparation using a Combined Brain Subcellular Fractionation/Co-Immunoprecipitation (co-IP) Procedures -- Co-Immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments -- Co-Immunoprecipitation (co-IP) Analysis for Protein-Protein Interactions in the Neurons of the Cerebral Ganglia of the Land Snails of the Genus Polymita during Aestivation -- Co-Immunoprecipitation (co-IP) of G Protein-Coupled Receptors (GPCR)-Receptor Tyrosine Kinases (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain
    Abstract: This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field
    Pages: XIII, 166 p. 28 illus., 19 illus. in color. : online resource.
    ISBN: 9781493989850
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    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Power of Drosophila Genetics: The Discovery of the Hippo Pathway -- Drosophila Genetics: The Power of Genetic Mosaic Approaches -- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues -- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs -- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence -- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions -- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells -- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells -- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors -- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells -- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells -- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays -- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids -- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview -- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels -- Hippo Pathway Regulation by Tyrosine Kinases -- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway -- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro -- GST Pull-Down Assay to Measure Complex Formations -- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag -- Quantifying the Kinase Activities of MST1/2 -- Measuring the Kinase Activities of the LATS/NDR Protein Kinases -- MST1/2 Kinase Assays Using Recombinant Proteins -- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development -- The Hippo Signaling Pathway in Regenerative Medicine
    Abstract: This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings
    Pages: XIV, 374 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493989102
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