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  • shoot regeneration  (33)
  • 1
    ISSN: 1573-5044
    Keywords: Vigna radiata ; shoot regeneration ; callus ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphogenetic responses of seedling explants of mung bean (Vigna radiata L. Wilczek cv ML-5) were studied in vitro. Direct induction of shoots/plants was possible from shoot tip, cotyledon and cotyledonary node explants. Dedifferentiation of the explants viz; Shoot tip, cotyledons, cotyledonary node, primordial leaves and roots was obtained on basal medium supplemented with auxin and cytokinin. Shoot regeneration was limited to primary calli while rhizogenesis was of common occurrence in established calli. In addition to differences in hormonal requirements, the various explants showed preferential growth in different basal media.
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  • 2
    ISSN: 1573-5044
    Keywords: geranium ; Pelargonium ; shoot regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Procedures have been developed that increase the rate of shoot regeneration of hybrid seed geranium from month-old primary callus cultures. Hybrid geranium callus tissue covered with green nodular structures was initiated by placing shoot tip explants on solidified Murashige & Skoog medium (MS) supplemented with 2.0 mgl-1 zeatin and 1.9 mgl-1 indoleacetic acid. Hybrids Red Orbit, White Orbit and Scarlet Orbit were shown to produce 5–50 shoot primordia per explant when callus was initiated on this medium. Regal geranium callus was initiated by placing leaf explants on MS medium supplemented with 2.0 mgl-1 6-benzylaminopurine and 2.0 mgl-1 naphthaleneacetic acid. Regal geranium cultivars Tiny Tot and Lavender Grand Slam were shown to produce between 2–50 shoot primordia per explant when initiated on the same medium.
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  • 3
    ISSN: 1573-5044
    Keywords: Lycopersicon ; callus ; organogenesis ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The organogenetic potential from callus of three tomato land races from the Canary Islands adapted to semi-arid environment (‘Salvaje’, ‘Rusa’ and ‘Especial’), and one tomato cultivar (‘Meltine’), were examined. The response of four explant types (cotyledon, shoot apex, hypocotyl and root) to nine PGR regimes (BAP at 1 or 2 or 5 mg/l) + either IAA (0.5 mg/l) or 2,4-D (0.5 or 1 mg/l) were measured. BAP at 5 mg/l+IAA at 0.5 mg/l induced most organogenesis in all the explant types for all genotypes. ‘Salvaje’ has one of the highest organogenetic potentials described in tomato.
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  • 4
    ISSN: 1573-5044
    Keywords: In vitro propagation ; Dracaena fragrans ; tissue culture ; callus ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus tissue was induced in young stem segments cultured on MS based media supplemented with 0.25–0.5 mg l-1 2, 4-D. Shoots were differentiated on media containing 0.5–1.0 mg l-1 BA and 0.5–2.0 mg l-1 IBA or 0.1–0.2 mg l-1 NAA. The same media were suitable for shoot multiplication. Shoot elongation and rooting were strongly inhibited by BA and stimulated by auxins IBA and NAA. Medium containing 0.5 mg l-1 IBA was optimal for rooting. Root elongation was stimulated by light and inhibited in darkness. Transfer of rooted plantlets to outdoor conditions was feasible and special hardening procedures were not required. Among more than 5000 plants produced by this procedure only 9 off-type plants with variegated leaves were found.
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  • 5
    ISSN: 1573-5044
    Keywords: plant growth regulators ; shoot regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol for high frequency adventitious shoot regeneration adventitious shoot regeneration from leaf explants of Rhododendron spp. has been developed. The highest percentage of regeneration and the greatest number of shoots were obtained when leaf explants were cultured on Anderson's medium containing 4.9 μM IBA and 73.8 μM 2iP. Genotypic variation was observed for adventitious shoot regeneration potential among the seven cultivars tested. Regeneration frequencies ranged from 0 to 96%. ‘Lodestar’ had the highest rate of regeneration after 3 months of culture with 96% shoot regeneration and an average of 14 shoots per explant. Regenerated shoots were rooted in soil in about 2 months. This protocol should be useful in applying gene transfer techniques to Rhododendron improvement.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 30 (1992), S. 247-249 
    ISSN: 1573-5044
    Keywords: Bromeliaceae ; endangered plant ; micropropagation ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; genotype effect ; GUS activity ; Lamiaceae ; lavandin ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five cultivars of lavandin were compared for their ability to regenerate plantlets in vitro and for their susceptibility to genetic transformation. Both processes were shown to be strongly cultivar-dependent. For regeneration, best results were obtained with cultivar ‘37–70’ which gave an average of 7 shoots from one initial explant after 4 months culture in vitro. The other cultivars produced between 0.5 and 3.5 shoots per explant. These differences were mostly due to the variable efficiency of the shoot elongation and rooting steps. An Agrobacterium-mediated transformation system using the β-glucuronidase and neomycin phosphotransferase II genes was established. The β-glucuronidase expression was analysed for both leaf explants six days after inoculation and kanamycin-resistant calluses obtained after a six-week culture on a selective medium. For each cultivar, kanamycin-resistant calluses showing a β-glucuronidase activity were obtained. The transformation efficiency ranged from 3% for cultivar ‘Certitude’ to 89% for cv. ‘41–70’ and ‘B–110’. Some kanamycin-resistant calluses were organogenic.
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  • 8
    ISSN: 1573-5044
    Keywords: Curcuma longa L. ; immature inflorescence ; shoot regeneration ; turmeric ; Zingiberaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration from cultured immature inflorescence of Curcuma longa was obtained by direct shoot development on Murashige and Skoog's basal medium supplemented with BA (5 or 10 mg l−1) in combination with 2,4-D (0.2 mg l−1) or NAA (0.1 mg l−1) and TDZ (1 or 2 mg l−1) in combination with IAA (0.1 mg l−1). Regenerated shoots were grown on MS medium for further development and later transferred to medium supplemented with 0.1 mg l−1 NAA for induction of roots. Complete plants thus obtained were transferred to sterilized soil in paper cups for 3–4 weeks and then to field, where 95% of the plants survived to maturity.
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  • 9
    ISSN: 1573-5044
    Keywords: osmoticum ; shoot regeneration ; Solanum melongena L. ; sugar requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uniform leaf pieces of egg-plant, Solanum melongena L., were cultured in Murashige and Skoog's medium containing 2 mg l-1 kinetin and varying sugar levels. Glucose or fructose at 44 mM was optimal in inducing shoot regeneration compared to sucrose. Sucrose at 11 and 22 mM induced more shoot organogenesis than at lower or higher levels. An additional 22 mM mannitol with 22 mM sucrose enhanced shoot regeneration significantly more than 22 mM sucrose alone. The dual role of sugar as carbon and osmotic source in shoot regeneration from leaf explants of egg-plant is discussed.
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  • 10
    ISSN: 1573-5044
    Keywords: benzyladenine ; Betula pendula Roth ; leaf explants ; organogenesis ; rejuvenation ; shoot regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The goal of this research was to develop a rapid and efficient system for regenerating shoots from leaf explants of European birch, Betula pendula Roth. Single-node stem explants were established in culture, and microshoots were subcultured every 4 weeks through 12 subcultures. Leaves from glasshouse plants or subcultured shoots were excised from stems, cut into approximately 35-mm2 pieces, and placed on Woody Plant Medium (WPM) containing different combinations of naphthaleneacetic acid (NAA) (0, 3, 6 or 9 μM) and benzyladenine (BA) (0, 7.5, 15 or 22.5 μM) in a 4×4 factorial design. The percentage of leaf pieces forming shoots and the number of shoots regenerated per explant were recorded after 4 weeks. Only media containing BA without NAA stimulated shoot formation on leaf explants. Fifteen micromolar BA induced the most shoots to form on leaf explants compared to 30, 45 or 60 μM of this cytokinin. In addition, shoot regeneration was enhanced up to four-fold between the first and eleventh subculture. Over 90% of the leaf explants regenerated shoots with an average of 18 buds formed per explant for the eleventh subculture. Almost twice as many explants formed shoots if their adaxial side was in contact with the medium rather than oriented away from it. The ability to regenerate shoots from leaves varied among plants, regardless of stock plant age. This reliable shoot regeneration system can be used for rapid shoot proliferation and potentially for genetic engineering of European birch.
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