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  • 1
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    Weinheim : Wiley
    Call number: QD33:14(2)
    Keywords: Chemistry
    Pages: xiii, 364 p.
    Edition: 2. Aufl.
    ISBN: 9783527345861
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    QD33:14(2) available
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  • 2
    Call number: Z070:502
    Pages: 223 p. : ill.
    Edition: 5., vollständig neu bearbeitete Auflage
    ISBN: 3-8007-4742-9
    Signatur Availability
    Z070:502 departmental collection or stack – please contact the library
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  • 3
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    München : Zuckschwerdt
    Call number: QZ203:109
    Keywords: Neoplasms / epidemiology ; Registries ; Germany ; epidemiology
    Pages: xiv, 219 p.
    ISBN: 9783863711658
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    QZ203:109 available
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  • 4
    Keywords: Economic development ; Economic policy ; Game theory ; Natural resources ; Regional Development ; Development Policy ; Game theory ; Natural Resource and Energy Economics ; Springer eBooks
    Description / Table of Contents: Chapter 1: Introduction -- Chapter 2: Reviewing the Literature and Setting Theoretical and Methodological Foundations for the Research -- Chapter 3: The Analytic Framework: Modeling the Dilemma of Transboundary River Basins as an Iterated PD Game -- Chapter 4: The Colonial Round: the Opening Scene of Utilizing the Nile Waters Outside Egypt -- Chapter 5: The Cold War Round: Large National Schemes on the Nile in a Polarized World -- Chapter 6: The Post Cold War Round: The Failure to Build an Institutionalized Basin-Wide Cooperation in the Nile -- Chapter 7: The Post-2011 Round: the New Chapter of the Grand Ethiopian Renaissance Dam and the Shadow of the Future -- Chapter 8: Conclusion: Tug-of-War or Benefits for All? -- Maps -- References
    Abstract: This book introduces an analytic framework constructed upon the iterated Prisoners' Dilemma game to model and analyze transboundary water interactions along the Nile River. It presents a thorough and in-depth analysis of the historical path through which conflict and cooperation have been generated among the Nile riparians over large-scale developmental schemes. This is done through modeling water interactions in the basin as an iterated Prisoners' Dilemma game and employing process-tracing method to compare four distinguishable rounds of the game: the colonial round, the Cold War round, the post-Cold War round, and the post-2011 round. The book examines the influences of the changing political contexts at the domestic, regional, and global levels on the game outcomes. This framework is initially applied on several cases of international rivers worldwide, while the rest of the book is devoted to the Nile case. The book's central argument is that the riparians' interests, capabilities, and beliefs are heterogeneous in varying degrees and that the changing multilevel political contexts influence the level of such heterogeneities among the riparians, which ultimately drive the equilibrium dynamics in the Nile game to generate different conflictive and cooperative outcomes over time. Although the book's main conclusion indicates that the absence of economic interdependence and regional integration will transfer the game into tug-of-war, which will impose harsh punishment on the basin communities and ecosystems on the long term, the final chapter lists a group of recommendations addressed to the riparian states and international donors, exploring the way for boosting cooperation and preventing conflicts in the basin. Presenting clear theoretical, methodological, and policy implications, this book is appropriate for students and scholars of international relations, hydrology, and development studies
    Pages: XXXIII, 277 p. 55 illus., 52 illus. in color. : online resource.
    ISBN: 9783030026653
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  • 5
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    Heidelberg : Springer
    Call number: QH442:222 ; ERW
    Keywords: Genetic Techniques
    ISBN: 9783642346934
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    QH442:222 available
    ERW in acquisition
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  • 6
    Call number: QZ200:527(11)
    Keywords: Neoplasms
    Notes: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Pages: xli, 2390 p. : ill.
    Edition: 11th ed.
    ISBN: 9781496394637
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    QZ200:527(11) available
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  • 7
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 8
    Keywords: Gene Therapy ; Medical virology ; Gene Therapy ; Virology ; Springer eBooks
    Description / Table of Contents: Basic Concepts in Viral Vector-Mediated Gene Therapy -- Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing -- Design, Construction, and Application of Transcription Activation-Like Effectors -- Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain -- AAV Production using Baculovirus Expression Vector System -- Multimodal Production of Adeno-Associated Virus -- Generation of High Titer Pseudotyped Lentiviral Particles -- A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration -- Current Use of Adenovirus Vectors and their Purification Methods -- Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest -- Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes -- AAV Mediated Gene Delivery to the Mouse Liver -- Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse -- Gene Transfer to Mouse Kidney In Vivo -- Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders -- Localized Intra-Arterial Gene Delivery using AAV -- Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors -- Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs -- Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons -- Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice -- Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates
    Abstract: This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments
    Pages: XIV, 328 p. 46 illus., 33 illus. in color. : online resource.
    ISBN: 9781493990658
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  • 9
    Keywords: Neurosciences ; Stem Cells ; Laboratory animals ; Neurosciences ; Stem Cells ; Animal Models ; Springer eBooks
    Description / Table of Contents: Generation of Neural Stem Cells from Induced Pluripotent Stem Cells -- Making NSC and Neurons from Patient Derived Tissue Samples -- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury -- Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells -- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells -- In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology -- Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons -- Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene -- Single-Cell Library Preparation of iPSC-derived Neural Stem Cells -- Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells -- Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons -- Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells -- Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach -- Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells -- Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation -- Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart -- Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion
    Abstract: This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIV, 239 p. 51 illus., 47 illus. in color. : online resource.
    ISBN: 9781493990078
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  • 10
    Keywords: Medicine ; Oncology ; Molecular Medicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Amplicon-based Targeted Next Generation Sequencing of Formalin Fixed Paraffin Embedded Tissue -- Library preparation using FFPE-derived Tumor DNA for High Throughput Hybridization-based Targeted or Exome Sequencing -- Bioinformatics Basics for High Throughput Hybridization-based Targeted DNA Sequencing From FFPE-Derived Tumor Specimens: From Reads to Variants -- Annotation of Variant Data from High Throughput DNA Sequencing from Tumor Specimens: Filtering Strategies to Identify Driver Mutations -- Biological Interpretation of Complex Genomic Data -- Clinical Validation of Targeted Solid Tumor Profiling -- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors -- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation -- Overview of Fusion Detection Strategies Using Next Generation Sequencing -- Clinical Application of Fusion Gene Detection Using Next-generation Sequencing and the NanoString Technology -- Pipeline for Integrated Microarray Expression Normalization Toolkit (PIMENTo) for Tumor Microarray Profiling Experiment -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: Overview of Library Preparation Methods -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: From Raw Data to Systems Level Analyses -- Methylation Analysis using Microarrays: Analysis and Interpretation -- High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) -- A Pipeline for ctDNA Detection Following Primary Tumor Profiling Using A Cancer-related Gene Sequencing Panel -- Single-cell Analysis of Circulating Tumor Cells
    Abstract: This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field
    Pages: XI, 268 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493990047
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  • 11
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 12
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: I-Cell Disease (Mucolipidosis II): A Case Series from a Tertiary Paediatric Centre Reviewing the Airway and Respiratory Consequences of the Disease -- Oral Ganglioside Supplement Improves Growth and Development in Patients with Ganglioside GM3 Synthase Deficiency -- Feeding Difficulties and Orofacial Myofunctional Disorder in Patients with Hepatic Glycogen Storage Diseases -- Auxiliary Partial Orthotopic Liver Transplantation for Monogenic Metabolic Liver Diseases: Single-Centre Experience -- A Novel Truncating FLAD1 Variant, Causing Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) in an 8-Year-Old Boy -- The Effect of Continuous Intravenous Glucagon on Glucose Requirements in Infants with Congenital Hyperinsulinism -- Case of Neonatal Fatality from Neuromuscular Variant of Glycogen Storage Disease Type IV -- Acute and Chronic Management in an Atypical Case of Ethylmalonic Encephalopathy -- Dihydropyrimidine Dehydrogenase Deficiency: Homozygosity for an Extremely Rare Variant in DPYD due to Uniparental Isodisomy of Chromosome 1 -- Severe Neonatal Manifestations of Infantile Liver Failure Syndrome Type 1 Caused by Cytosolic Leucine-tRNA Synthetase Deficiency -- Enzyme Replacement Therapy in Pregnant Women with Fabry Disease: A Case Series -- Survival of a Male Infant with a Familial Xp11.4 Deletion Causing Ornithine Transcarbamylase Deficiency -- The Unique Spectrum of Mutations in Patients with Hereditary Tyrosinemia Type 1 in Different Regions of the Russian Federation -- Elevated Lyso-Gb3 Suggests the R118C GLA Mutation Is a Pathological Fabry Variant -- Glycogen Storage Disease Type IV: A Rare Cause for Neuromuscular Disorders or Often Missed? -- A Hemizygous Deletion Within the PGK1 Gene in Males with PGK1 Deficiency
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 110 p. 28 illus., 13 illus. in color. : online resource.
    ISBN: 9783662586471
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  • 13
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Plant genetics ; Bacteriology ; Bioinformatics ; Human Genetics ; Microbial Genetics and Genomics ; Microbial ecology ; Plant Genetics and Genomics ; Bacteriology ; Bioinformatics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Part 1. Concepts and Approaches -- Chapter1. Computational Methods in Microbial Population Genomics -- Chapter2. What Microbial Population Genomics has taught us about Speciation -- Chapter3. Peering into the genetic makeup of natural microbial populations using metagenomics -- Chapter4. A reverse ecology framework for bacteria and archaea -- Part2. Population genomics of bacteria and archaea -- Chapter5. What is a Pseudomonas syringae Population? -- Chapter6. An introductory narrative to the population genomics of pathogenic bacteria, exemplified by Neisseria meningitidis -- Chapter7. Population genomics of archaea: Signatures of archaeal biology from natural populations -- Part3. Population genomics of fungi -- Chapter8. Advances in Genomics of Human Fungal Pathogens -- Chapter9. Yeast population genomics goes wild: The case of Saccharomyces paradoxus -- Part4. Population genomics of viruses -- Chapter10. Population genomics of plant viruses -- Chapter11. Population genomics of human viruses -- Chapter12. Population genomics of bacteriophages
    Abstract: Population genomics is a rapidly emerging field that has the potential to transform our understanding of how evolutionary forces shape genomic diversity among microbes. There have already been considerable advances in understanding gene flow and spread of adaptive traits, and in linking epidemiology with evolutionary biology. The current challenge is to find unifying evolutionary principles for organisms that display a wide range of reproductive biology – from highly clonal to promiscuous – and for which the vast majority have eluded cultivation. This requires interdisciplinary approaches that incorporate novel computational tools, testing of existing and novel population genetic models, and creative new ways of linking genetic diversity to ecological factors. This pioneering book will discuss the advances made and promises of population genomics in microorganisms, outlining some of the key theoretical and practical challenges for microbial population genomics, including defining and identifying populations, genomics-based reverse ecology and building appropriate tools to understand microbes in a variety of complex environments
    Pages: XIII, 344 p. : online resource.
    ISBN: 9783030047566
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  • 14
    Keywords: Plant genetics ; Ecology ; Plant breeding ; Plant anatomy ; Plant Ecology ; Medicine ; Plant Genetics and Genomics ; Community & Population Ecology ; Plant Breeding/Biotechnology ; Plant Anatomy/Development ; Plant Ecology ; Health Promotion and Disease Prevention ; Springer eBooks
    Description / Table of Contents: Part1. Introduction -- Chapter1. Population genomics: Advancing understanding of nature -- Part2. Methods -- Chapter2. Genotyping and sequencing technologies in population genetics and genomics -- Chapter3. Computational tools for population genomics -- Chapter4. Population and evolutionary genetic inferences in the whole genome era: Software challenges -- Part3. Concepts and Approaches -- Chapter5. Population epigenomics: Advancing understanding of phenotypic plasticity, acclimation, adaptation and diseases -- Chapter6. Landscape genomics: Understanding heterogeneity and genomic characteristics of populations -- Chapter7. Paleogenomics: Genome-scale analysis of ancient DNA and population and evolutionary genomic inferences -- Chapter8. Genome-wide association studies and heritability estimation in the functional genomics era -- Chapter9. Genomic selection -- Part4. -- Chapter10. Population genomics provides key insights in ecology and evolution -- Chapter11. Inferring demographic history using genomic data -- Chapter12. Advancing biogeography through population genomics -- Chapter13. Adaptation without boundaries: Population genomics in ocean systems -- Chapter14. Population genomics of speciation and admixture -- Chapter15. Population genomics of colonization and invasion -- Chapter16. Population genomics of crop domestication: current state and perspectives -- Chapter17. Population genomics of animal domestication and breed development -- Chapter18. Population genomics of domestication and breed development in canines in the context of cognitive, social, behavioral, and disease traits
    Abstract: Population genomics has revolutionized various disciplines of biology including population, evolutionary, ecological and conservation genetics, plant and animal breeding, human health, medicine and pharmacology by allowing to address novel and long-standing questions with unprecedented power and accuracy. It employs large-scale or genome-wide genetic information and bioinformatics to address various fundamental and applied aspects in biology and related disciplines, and provides a comprehensive genome-wide perspective and new insights that were not possible before. These advances have become possible due to the development of new and low-cost sequencing and genotyping technologies and novel statistical approaches and software, bioinformatics tools, and models. Population genomics is tremendously advancing our understanding the roles of evolutionary processes, such as mutation, genetic drift, gene flow, and natural selection, in shaping up genetic variation at individual loci and across the genome and populations; improving the assessment of population genetic parameters or processes such as adaptive evolution, effective population size, gene flow, admixture, inbreeding and outbreeding depression, demography, and biogeography; resolving evolutionary histories and phylogenetic relationships of extant, ancient and extinct species; understanding the genomic basis of fitness, adaptation, speciation, complex ecological and economically important traits, and disease and insect resistance; facilitating forensics, genetic medicine and pharmacology; delineating conservation genetic units; and understanding the genetic effects of resource management practices, and assisting conservation and sustainable management of genetic resources. This Population Genomics book discusses the concepts, approaches, applications and promises of population genomics in addressing most of the above fundamental and applied crucial aspects in a variety of organisms from microorganisms to humans. The book provides insights into a range of emerging population genomics topics including population epigenomics, landscape genomics, seascape genomics, paleogenomics, ecological and evolutionary genomics, biogeography, demography, speciation, admixture, colonization and invasion, genomic selection, and plant and animal domestication. This book fills a vacuum in the field and is expected to become a primary reference in Population Genomics world-wide
    Pages: XVII, 822 p. 110 illus., 85 illus. in color. : online resource.
    ISBN: 9783030045890
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  • 15
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    New York : Basic Books
    Call number: G901:12
    Keywords: Artificial intelligence ; Medical Informatics ; Diagnosis, Computer-Assisted ; Therapy, Computer-Assisted ; Quality Improvement
    Description / Table of Contents: Introduction to deep medicine -- Shallow medicine -- Medical diagnosis -- The skinny on deep learning -- Deep liabilities -- Doctors and patterns -- Clinicians without patterns -- Mental health -- AI and health systems -- Deep discovery -- Deep diet -- The virtual medical assistant -- Deep empathy
    Pages: xiii, 378 p. : ill.
    ISBN: 9781541644632
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    G901:12 departmental collection or stack – please contact the library
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  • 16
    Call number: QZ269:203(3)
    Keywords: Radiation Oncology / methods ; Neoplasms / radiotherapy
    Pages: xxviii, 713 p. : ill.
    Edition: 3rd ed.
    ISBN: 9780128141281
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  • 17
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    Berlin : de Gruyter
    Call number: Z699.A2:117/Zi.D124b
    Pages: xvi, 448 p. : ill.
    ISBN: 9783110525878
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  • 18
    Keywords: Biomedical Engineering ; Biology / Data processing ; Biotechnology ; Biomedical Engineering/Biotechnology ; Computer Appl. in Life Sciences ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Enhancing Nursing Education through Affordable and Realistic Holographic Mixed Reality: The Virtual Standardized Patient for Clinical Simulation -- Potential Application Of Virtual Reality For Interface Customisation (And Pre-Training) Of Amputee Patients As Preparation For Prosthetic Use -- Visualising Medical Heritage: New Approaches To Digitisation And Interpretation Of Medical Heritage Collections -- Integrating 3D visualisation technologies in undergraduate anatomy education -- Pedagogical perspectives on the use of technology within medical curricula: moving away from norm driven implementation -- Towards a More User-Friendly Medication Information Delivery to People Living with Multiple Sclerosis: A Case Study with Alemtuzumab -- The Co-Design of Hand Rehabilitation Exercises for Multiple Sclerosis Using Hand Tracking System -- Examining Vascular Structure And Function Using Confocal Microscopy And 3D Imaging Techniques -- Which Tool Is Best: 3D Scanning Or Photogrammetry - It Depends On The Task -- Application of Photogrammetry in Biomedical Science
    Abstract: This edited volume explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in imaging and analysis, education, engagement and training. The reader will be able to explore the utilisation of technologies from a number of fields to enable an engaging and meaningful visual representation of the life sciences. This use of technology-enhanced learning will be of benefit for the learner, trainer, in patient care and the wider field of education and engagement. By examining a range of techniques in image capture (photogrammetery, stereophotogrammetry, microphotogrammetry and autostereoscopy), this book will showcase the wide range of tools we can use. Researchers in this field will be able to find something suitable to apply to their work to enhance user engagement through improved visual means using the technologies we have available to us today. It will highlight the uses of these technologies to examine many aspects of the human body, and enable improved ways to enhance visual and tactile learning, including 3D printing. By demonstrating co-design processes, working directly with the end-stage users (including patients), it will also highlight successes in adopting tools like hand motion tracking rehabilitation for patients with conditions like multiple sclerosis. The book will also discuss the applications of immersive environments including virtual, augmented and mixed reality. The ultimate aim is to show how, by using these tools, we can enhance communication, mobile applications, health literacy and illustration of both normal and pathological processes in the body. By applying a wide range of tools and technologies, this volume will highlight the wide range of applications in education, training and learning both for students and faculty, but also for patient care and education. Therefore, the work presented here can be accessed by a wide range of users from faculty and students involved in the design and development of these processes, by examining the pedagogy around these technologies. Importantly, it presents material, which will be of benefit for the patient, engaging them to become more involved with techniques like physiotherapy
    Pages: XV, 132 p. 65 illus., 42 illus. in color. : online resource.
    ISBN: 9783030060701
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  • 19
    Keywords: Human Physiology ; Human Physiology ; Springer eBooks
    Abstract: In recent years, volumes of the long-established Springer Subcellular Biochemistry series have shown great diversity of content, thus interesting biochemical topics, enthusiastically edited in all cases, have been readily accommodated. This new volume of the Subcellular Biochemistry series, Biochemistry and Cell Biology of Ageing: Part II, offers a broad overview of many different Clinical Science aspects of Ageing. The 17 chapters included in the book, contributed by knowledgeable authors, review many important topics at an advanced level. Whilst it has proved impossible to include all relevant topics, there is much present that will be of value to the reader. The book contains chapters on: “Poor Early Growth and Age-associated Disease; The Immune System and its Dysregulation with Ageing; Pulmonary Diseases and Ageing; Neurodegenerative Diseases and Ageing; Ageing and Cognition; Ageing and Osteoarthritis; Down Syndrome, Ageing and Epigenetics; The Vestibular System and Ageing; Signal Transduction, Ageing and Disease; Skin Changes During Ageing; Connective Tissue and Age-related Diseases ; Potential Cellular and Biochemical Mechanisms of Exercise and Physical Activity on the Ageing Process; Health Benefits of Anti-ageing Drugs; Visual Defects and Ageing; Hutchinson-Gilford Progeria Syndrome: Challenges at Bench and Bedside; Osteoporosis and the Ageing Skeleton; Neurovascular Ageing and Age-related Diseases” The book is primarily directed to advanced biomedical science and medical students, postgraduates, researchers, clinicians and academics in the field of Ageing. An earlier companion volume of the Subcellular Biochemistry series - Biochemistry and Cell Biology of Ageing: Part I (Volume 90) - covering the Biomedical Science aspects of ageing, has also been recently published
    Pages: X, 499 p. 53 illus., 47 illus. in color. : online resource.
    ISBN: 9789811336812
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  • 20
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Left Ventricular Strain and Relaxation are Independently Associated with Renal Cortical Perfusion in Hypertensive Patients -- Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: a Bispectral Analysis -- Influence of Heart Rate, Age, and Gender on Heart Rate Variability in Adolescents and Young Adults -- Hand-Foot Syndrome and Progression-Free Survival in Patients Treated with Sunitinib for Metastatic Clear Cell Renal Cell Carcinoma -- Elastography in the Diagnosis of Pancreatic Malignancies -- Factors Affecting Health-Related Quality of Life in Liver Transplant Patients -- Phagocytosis and Autophagy in THP-1 cells Exposed to Urban Dust: Possible Role of LC3€“Ássociated Phagocytosis and Canonical Autophagy -- Gendeŕ€“Dependent Responses of Growth and Insuliń€“like Growth Factoŕ€“1 to Growth Hormone Therapy in Prepubertal Growth Hormone Deficient Children -- Vitamin D and Calcium Homeostasis in Infants with Urolithiasis -- Morphometric Analysis of the Lumbar Vertebrae Concerning the Optimal Screw Selection for Transpedicular Stabilization -- Stress and Dehumanizing Behaviors of Medical Staff toward Patients -- Biological and Social Determinants of Maximum Oxygen Uptake in Adult Men
    Abstract: This book focuses on issues in clinical practice and research that are of general interest. The articles primarily focus on understanding the pathogenic mechanisms of diseases, their prevention, and therapy. The topics addressed include cardiovascular regulation with regard to blood pressure and heart rate variability, and to coupling blood pressure changes with subarachnoid fluid oscillations. In addition, the book discusses recent advances in the diagnostics of and targeted molecular therapy for renal and pancreatic malignancies, growth disorders, vitamin D and calcium homeostasis in children in the context of neonatal urolithiasis, and neurosurgical interventions in multifarious age-related diseases of the vertebrae. Neuropsychological aspects of patients’ quality of life and of shaping medical staff’s attitude toward patients are also addressed. The respective articles are intended to build a bridge between basic and clinical research. Further, the book enhances the current body of knowledge on diagnostics and patient treatment and offers valuable new perspectives on practical clinical issues. As such, it offers a unique resource for clinicians, family physicians, medical scholars, and professionals engaged in patient management
    Pages: VI, 114 p. 20 illus. in color. : online resource.
    ISBN: 9783030129231
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  • 21
    Keywords: Biochemistry ; Neurosciences ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease
    Abstract: This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects
    Pages: XII, 289 p. 66 illus., 55 illus. in color. : online resource.
    ISBN: 9781493991242
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  • 22
    Keywords: Cell Culture ; Laboratory animals ; Cell Culture ; Animal Models ; Springer eBooks
    Description / Table of Contents: The Applicability of Mouse Models to the Study of Human Disease -- Optimizing the Cell Culture Microenvironment -- Propagation and Maintenance of Mouse Embryonic Stem Cells -- Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells -- Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction -- Gene Editing of Mouse Embryonic and Epiblast Stem Cells -- Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood -- Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture -- Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation -- Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo -- Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells -- Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells -- Organotypic Culture of Adult Mouse Retina -- Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes -- Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes -- Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells -- An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells -- Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans -- Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers -- The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential -- Mouse Lung Tissue Slice Culture
    Abstract: This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets
    Pages: XII, 315 p. 64 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990863
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  • 23
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    New York : Springer
    Keywords: Medicine ; Orthopedics ; Molecular Medicine ; Orthopedics ; Springer eBooks
    Description / Table of Contents: Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone
    Abstract: This 3rd edition book provides are large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research
    Pages: XV, 670 p. 172 illus., 121 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493989973
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  • 24
    Keywords: Pharmaceutical technology ; Pharmacy ; Nanochemistry ; Pharmaceutical Sciences/Technology ; Pharmacy ; Nanochemistry ; Springer eBooks
    Description / Table of Contents: Understanding the Surface Characteristics of Nanoparticles -- Surface Modification of Nanoparticles for Targeted Drug Delivery -- Surface-Modified PLGA Nanoparticles for Targeted Drug Delivery to Neurons -- Nanoparticles for Site Specific Drug Delivery -- Stealth Properties of Nanoparticles against Cancer: Surface Modification of NPs for Passive Targeting to Human Cancer Tissue in Zebra Fish -- Passive targeting of nanoparticles to cancer -- Surface Modification of Magnetic Nanoparticles in Biomedicine -- A novel strategy for the surface modification of super paramagnetic (Fe3O4) iron oxide nanoparticle for lung cancer imaging -- Biodegradable nanoparticles for drug delivery and targeting -- Functionalized antibacterial nanoparticles in controlling biofilm and intracellular infections -- Surface modifications of liposomes for drug targeting -- Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system -- Surface Modification of Metallic Nano particles -- Successful Delivery of Zidovudine-loaded Docosanol Nanostructured Lipid Carriers (Docosanol NLCs) into rat brain -- Brain targeted drug delivery with surface modified nanoparticles -- Surface Modification of Resorcinarene Based Self-Assembled Solid Lipid Nanoparticles for Drug Targeting -- Targeted delivery of surface modified nanoparticles:Modulation of inflammation for acute lung injury -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface Modification of Gold Nanoparticles for Targeted Drug Delivery -- Surface modification and bioconjugation of nanoparticles for MRI technology -- Surface Modified Lanthanide Nanomaterials for Drug Delivery -- Engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers -- Application of Nanoparticles in treating Periodontitis: Preclinical and Clinical overview -- Surface Modification of Nanoparticles for Ocular Drug Delivery
    Abstract: This unique book discusses various new techniques developed to enhance the application of nanoparticulate drug delivery systems using surface modification of nanoparticles. The understanding of the surface characteristics of nanoparticles is growing significantly with the advent of new analytical techniques. Polymer chemistry is contributing to the development of many new versatile polymers which have abilities to accommodate many different, very reactive chemical groups, and can be used as a diagnostic tool, for better targeting, for more effective therapeutic results as well as for reducing the toxic and side effects of the drugs. Surface modification of such polymeric nanoparticles has been found by many scientists to enhance the application of nano-particles and also allows the nanoparticles to carry specific drug molecule and disease /tumor specific antibodies which refine and improve drug delivery. Surface Modification of Nanoparticles for Targeted Drug Delivery is a collection of essential information on various applications of surface modification of nano-particles and their disease specific applications for therapeutic purposes
    Pages: XVIII, 499 p. 142 illus., 110 illus. in color. : online resource.
    ISBN: 9783030061159
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  • 25
    Keywords: Bacteriology ; Vaccines ; Emerging infectious diseases ; Bacteriology ; Vaccine ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: An Overview of Neisseria meningitides -- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR -- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis -- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics -- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis -- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis -- Sample Preparation for Proteomic Analysis of Neisseria meningitides -- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance -- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. -- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells -- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease -- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays -- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines -- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine -- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides -- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis
    Abstract: This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen
    Pages: XIII, 253 p. 58 illus., 42 illus. in color. : online resource.
    ISBN: 9781493992027
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  • 26
    Keywords: Bacteriology ; Enzymes ; Immunology ; Bacteriology ; Enzymology ; Immunology ; Springer eBooks
    Description / Table of Contents: 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus
    Abstract: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets
    Pages: XIII, 312 p. 70 illus., 41 illus. in color. : online resource.
    ISBN: 9781493991549
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  • 27
    Keywords: Biochemistry ; Cytology ; Protein Science ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cloning and Mutagenesis Strategies for Large Collagens -- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen -- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds -- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils -- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy -- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry -- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes -- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics -- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy -- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology -- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics -- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix -- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans -- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis -- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment -- FACS Analysis of Col1α Protein Levels in Primary Fibroblasts -- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors
    Abstract: This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study
    Pages: XI, 254 p. 60 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990955
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  • 28
    Keywords: Pharmaceutical technology ; Bioinformatics ; Pharmaceutical Sciences/Technology ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Miniaturized Checkerboard Assays to Measure Antibiotic Interactions -- High-Throughput Screening for Drug Combinations -- Post-Processing of Large Bioactivity Data -- How to Develop a Drug Target Ontology: KNowledge Acquisition and Representation Methodology (KNARM) -- A Guide to Dictionary-Based Text Mining -- Leveraging Big Data to Transform Drug Discovery -- How to Prepare a Compound Collection Prior to Virtual Screening -- Building a Quantitative Structure-Property Relationship (QSPR) Model -- Isomeric and Conformational Analysis of Small Drug and Drug-Like Molecules by Ion Mobility Mass Spectrometry (IM-MS) -- A Computational Platform and Guide for Acceleration of Novel Medicines and Personalized Medicine -- Omics Data Integration and Analysis for Systems Pharmacology -- Bioinformatics Based Tools and Software in Clinical Research: A New Emerging Area -- Text Mining for Drug Discovery -- Big Data Cohort Extraction for Personalized Statin Treatment and Machine Learning -- Drug Signature Detection Based on L1000 Genomic and Proteomic Big Data -- Drug Effect Prediction by Integrating L1000 Genomic and Proteomic Big Data -- A Bayesian Network Approach to Disease Subtype Discovery
    Abstract: This third edition volume expands on the previous editions with new topics that cover drug discovery through translational bioinformatics, informatics, clinical research informatics, as well as clinical informatics. The chapters discuss new methods to study target identification, genome analysis, cheminformatics, protein analysis, and text mining. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, software workflows, reagents and on-line resources, together with step-by-step, readily reproducible laboratory and computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Bioinformatics and Drug Discovery, Third Edition is a valuable resource for anyone interested in drug design, including academicians (biologists, informaticists and data scientists, chemists, and biochemists), clinicians, and pharmaceutical scientists
    Pages: XI, 324 p. 63 illus., 54 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493990894
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  • 29
    Keywords: Immunology ; Laboratory animals ; Immunology ; Animal Models ; Springer eBooks
    Description / Table of Contents: CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells -- Genome Editing in Mouse Embryos with CRISPR/Cas9 -- Derivation of Macrophages from Mouse Bone Marrow -- Bone Marrow Derived Dendritic Cells -- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils -- In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets -- Generation and Culture of Mouse Embryonic Fibroblasts -- Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation -- Depletion and Reconstitution of Macrophages in Mice -- Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making -- A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colz -- Mouse Model of Staphylococcus aureus Skin Infection -- Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses -- Endotoxin-Induced Uveitis in Rodents -- Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice -- Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages -- Influenza-Mediated Lung Infection Models -- Adoptive Transfer Colitis -- The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC) -- Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA) -- Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions -- Sepsis Induced by Cecal Ligation and Puncture
    Abstract: This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system
    Pages: XI, 258 p. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991679
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  • 30
    Keywords: Pharmaceutical technology ; Biomedical Engineering ; Pharmaceutical Sciences/Technology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Molecular Biotechnology -- Biophysical and biochemical analysis of recombinant proteins -- Production and downstream processing of biotech products -- Formulation of biotech products, including biopharmaceutical considerations -- Pharmacokinetics and pharmacodynamics of peptide and protein drugs -- Immunogenicity of pharmaceutical proteins -- General considerations of monoclonal antibodies from structure to therapeutic application -- Genomics, Other €œÓmicś€ Technologies, Personalized Medicine and Additional Biotechnology-Related Techniques -- Dispensing biotechnology products: handling, professional education and product information -- Economic considerations in medical biotechnology -- Regulatory framework for biosimilars -- Vaccines -- Oligonucleotides and siRNA -- Gene therapy -- Stem cell technology -- Therapeutic Applications.-Endocrinology -- Insulin -- Follicle-stimulating hormone -- Growth hormone -- Cardiovascular and Respiratory Applications -- Recombinant coagulation factors and thrombolytics -- Recombinant human deoxyribonuclease -- Oncology -- Monoclonal antibodies in oncology -- Hematopoetic growth factors -- Inflammation and Immunemodulation -- Monoclonal antibodies in transplantation -- Monoclonal antibodies and antibody-based therapeutics in anti-inflammatory therapy -- Interferons and interleukins -- Anti-infectious diseases -- Monoclonal antibodies -- Enzyme replacement therapy
    Abstract: This introductory text explains both the basic science and the applications of biotechnology derived pharmaceuticals, with special emphasis on their clinical use. It serves as a complete one-stop source for undergraduate/graduate pharmacists, pharmaceutical science students, and for those who did not receive formal training in this field. Pharmaceutical Biotechnology: Fundamentals and Applications, Fifth Edition completely updates the previous edition, and includes additional coverage on newer approaches such as oligonucleotides, mi/siRNA, gene therapy and advanced therapeutics. Daan J.A. Crommelin is Professor Emeritus Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences at Utrecht University, Utrecht, The Netherlands and Adjunct Professor in the Department of Pharmaceutics and Pharmaceutical Chemistry at the University of Utah, Salt Lake City, UT, USA Robert D. Sindelar is Professor and former Dean Faculty of Pharmaceutical Sciences and The Centre for Health Evaluation & Outcomes Sciences (CHEOS), The University of British Columbia (UBC), Vancouver, BC, Canada and President of the Global Drug Commercialization Centre (GDCC)-China and Vice-President GDCC-worldwide, Chengdu, China Bernd Meibohm is Professor of Pharmaceutical Sciences and Associate Dean for Research and Graduate Programs at the University of Tennessee Health Science Center, College of Pharmacy, Memphis, TN, USA
    Pages: XXIII, 653 p. 246 illus., 227 illus. in color. : online resource.
    Edition: 5th ed. 2019.
    ISBN: 9783030007102
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  • 31
    Keywords: Toxicology ; Biochemistry ; Oncology ; Pharmacology/Toxicology ; Biological and Medical Physics, Biophysics ; Biochemistry, general ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- Chapter 1. Antimicrobial peptides of multicellular organisms: My perspective -- Part 2. Mechanisms of Antimicrobial Action -- Chapter 2. Membrane permeabilization mechanisms -- Chapter 3. Elementary processes and mechanisms of interactions of antimicrobial peptides with membranes --- single GUV studies ---- -- Chapter 4. The mechanisms of action of cationic antimicrobial peptides refined by novel concepts from biophysical investigations -- Chapter 5. Anionic lipid clustering model -- Chapter 6. Intracellular antimicrobial peptides targeting the protein synthesis machinery -- Part 3. Other activities of AMPs -- Chapter 7. Antimicrobial and cell-penetrating peptides: How to understand two distinct functions despite similar physicochemical properties -- Chapter 8. Synthetic anti-lipopolysaccharide peptides (SALP) as effective inhibitors of pathogen-associated molecular patterns (PAMPs) -- Chapter 9. Anti-cancer activities of natural and synthetic peptides -- Chapter 10. Antimicrobial host defence peptides: Immunomodulatory functions and translational prospects -- Part 4. Towards Clinical Applications -- Chapter 11. Activity and selectivity of antimicrobial peptides: A complex interplay of multiple equilibria -- Chapter 12. Design of antimicrobial peptides: Progress made with human cathelicidin LL-37 -- Chapter 13. Application of synthetic molecular evolution to the discovery of antimicrobial peptides -- Chapter 14. AMPs as anti-biofilm agents for human therapy and prophylaxis -- Chapter 15. Clinical application of AMPs
    Abstract: This book presents an overview of antimicrobial peptides (AMPs), their mechanisms of antimicrobial action, other activities, and various problems that must still be overcome regarding their clinical application. Divided into four major parts, the book begins with a general overview of AMPs (Part I), and subsequently discusses the various mechanisms of antimicrobial action and methods for researching them (Part 2). It then addresses a range of activities other than antimicrobial action, such as cell penetration, antisepsis, anticancer, and immunomodulatory activities (Part 3), and explores the prospects of clinical application from various standpoints such as the selective toxicity, design, and discovery of AMPs (Part 4). A huge number of AMPs have been discovered in plants, insects, and vertebrates including humans, and constitute host defense systems against invading pathogenic microorganisms. Consequently, many attempts have been made to utilize AMPs as antibiotics. AMPs could help to solve the urgent problem of drug-resistant bacteria, and are also promising with regard to sepsis and cancer therapy. Gathering a wealth of information, this book will be a bible for all those seeking to develop antibiotics, anti-sepsis, or anticancer agents based on AMPs
    Pages: VIII, 304 p. 73 illus., 55 illus. in color. : online resource.
    ISBN: 9789811335884
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  • 32
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    Cham : Springer
    Keywords: Biological models ; Human Genetics ; Endocrinology ; Cytology ; Systems Biology ; Human Genetics ; Endocrinology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: 1. History of the thymus: From a vestigial organ to the programming of immunological self-tolerance -- 2. Thymus Ontogeny and Development -- 3. The Ins and Outs of Thymic Epithelial Cell Differentiation and Function -- 4. T-Cell Development: From T-Lineage Specification to Intrathymic Maturation -- 5. Intrathymic cell migration: implications in thymocyte development and T-cell repertoire formation -- 6. Thymic crosstalk: an overview of the complex cellular interactions that control the establishment of T-cell tolerance -- 7. The Autoimmune Regulator (AIRE) gene, the master activator of self-antigen expression in the thymus -- 8. Aire Mutations and Autoimmune Diseases -- 9. The thymus as a mirror of the body's gene expression -- 10. Functional genomics of the infant human thymus: Aire and minipuberty -- 11. Thymus transcriptome of TGF-β superfamily -- 12. Development of thymic regulatory T lymphocytes -- 13. Adding insult to injury: improving the regenerative capacity of the aged thymus following clinically induced damage -- 14. Thymus rejuvenation after autologous hematopoietic stem cell transplantation in patients with autoimmune diseases
    Abstract: This volume focuses on challenging field in biomedicine that is the genetic control of central immune tolerance. It covers the thymus development, their cellular components and their respective function, the peculiar gene expression profiling (transcriptome) found in the medullary thymic epithelial cells (mTECs) that are implicated in the self-representation in the thymus, the Autoimmune regulator (Aire) gene, the mutations in this gene and manifestation of autoimmune diseases, and the role of cell-cell interactions within the thymus with implications in the negative selection (elimination) of nascent autoreactive T cells in preventing aggressive autoimmunity. The thymus gland is a lymphoid organ implicated in the maturation, differentiation and selection of T cells. This organ is gained more and more attention in different biomedical research labs worldwide due to its function that is associated with the control of immune homeostasis in the body, establishing the central immune tolerance and preventing the onset of autoimmune diseases
    Pages: XVII, 318 p. 30 illus., 23 illus. in color. : online resource.
    ISBN: 9783030120405
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  • 33
    Keywords: Human Genetics ; Bioinformatics ; Human Genetics ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Chapter1. Strategies for converting RNA to amplifiable cDNA for single-cell RNA sequencing methods -- Chapter2. Integrated Fluidic Circuits for single cell omics and multi-omics applications -- Chapter3. Single-cell DNA-Seq and RNA-Seq in cancer using the C1 system -- Chapter4. Nx1-Seq (Well based Single-Cell Analysis System) -- Chapter5. Quantitation of mRNA transcripts and proteins using the BD Rhapsody™ Single-Cell Analysis System -- Chapter6. An Informative Approach to Single-Cell Sequencing Analysis -- Chapter7. Bionano Genome Mapping: High-Throughput, Ultra-Long Molecule Genome Analysis System for Precision Genome Assembly and Haploid-Resolved Structural Variation Discovery -- Chapter8. Informatics for PacBio long reads -- Chapter10. On-site MinION sequencing
    Abstract: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians
    Pages: VI, 150 p. 56 illus., 51 illus. in color. : online resource.
    ISBN: 9789811360374
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  • 34
    facet.materialart.
    C.H.Beck
    Call number: ERW
    Edition: 7. Aufl.
    ISBN: 9783406679971
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  • 35
  • 36
    Call number: M280:2
    Keywords: Civil law / Germany ; Droit civil / Allemagne
    Pages: xxxi, 833 p.
    Edition: 83., überarb. Aufl., Stand: 15. Januar 2019
    ISBN: 9783423050012
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    M280:2 departmental collection or stack – please contact the library
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  • 37
    Keywords: Stem Cells ; Oncology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Pericytes in the retina -- Pancreatic pericytes in glucose homeostasis and diabetes -- Pericytes in the lung -- Pericytes in the skeletal muscle -- Pericytes in the gut -- Pericytes in the bone marrow -- Cochlear Capillary Pericytes -- Pericytes in the placenta: role in placental development and homeostasis -- Pericytes in the liver -- Pericytes in the Periodontal Ligament -- Pericytes in the heart -- Pericytes in the Umbilical Cord -- The pluripotent microvascular pericytes are the adult stem cells even in the testis -- Index
    Abstract: The present book is an attempt to describe the most recent developments in the area of pericyte biology which is one of the emergent hot topics in the field of molecular and cellular biology today. Here, we present a selected collection of thirteen detailed chapters on what we know so far about pericytes in distinct organs in physiological and pathological conditions. Further, it provides an update on the most novel functions attributed to these cells and will introduce a newer generation of researchers and scientists to the importance of these cells, ranging from their discovery in different organs through current state-of-the-science. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and vascular research. This volume explores pericytes' physiologic roles in different tissues, ranging from the pancreas, lungs and liver through skeletal muscle, gut, retina and more. Together with its companion volumes Pericyte Biology in Disease and Pericyte Biology – Novel Concepts, Pericyte Biology in Different Organs presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and researchers or clinicians involved with specific organs
    Pages: XI, 277 p. 60 illus., 51 illus. in color. : online resource.
    ISBN: 9783030110932
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  • 38
    Keywords: Biomedical Engineering ; Cytology / Research ; Microbial ecology ; Microbiology ; Plant Diseases ; Biomedical Engineering/Biotechnology ; Biological Techniques ; Microbial ecology ; Microbiology ; Plant Pathology ; Springer eBooks
    Description / Table of Contents: Part I: Root symbioses -- Chapter 1. Synthetic plasmids to challenge symbiotic nitrogen fixation between rhizobia and legumes -- Chapter 2. In vivo analysis of rhizosphere enzyme activities by the use of plastic syringes -- Chapter 3. Characterization of arbuscular mycorrhizal communities in roots of vineyard plants -- Chapter 4. Molecular methods for research on actinorhiza -- Chapter 5. Molecular and functional characterization of beneficial bacteria associated with AMF spores -- Part II: Plant pathogens and microbial plant protection -- Chapter 6. Oomycete-root interactions -- Chapter 7. Exploitation of rhizosphere microbiome services -- Chapter 8. Methods for detecting biocontrol and plant growth-promoting traits in rhizobacteria -- Chapter 9. A split-root method to study systemic and heritable traits induced by Trichoderma in tomato plants -- Part III: Experimental approaches and analytical techniques in rhizosphere biology -- Chapter 10. Using a tri-isotope (13C, 15N, 33P) labelling method to quantify rhizodeposition -- Chapter 11. Microscopic techniques coupled to molecular and genetic approaches to highlight cell-type specific differences in mycorrhizal symbiosis -- Chapter 12. From imaging to functional traits in interactions between roots and microbes -- Chapter 13. Live imaging of arbuscular mycorrhizal symbiosis -- Chapter 14. Microcosm approaches to investigate multitrophic interactions in microbial communities -- Chapter 15. Analysis of common mycorrhizal networks in microcosms
    Abstract: This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation
    Pages: IX, 279 p. 37 illus., 34 illus. in color. : online resource.
    ISBN: 9789811357671
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  • 39
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    New York, NY : Springer
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: Nonsurgical Hammertoe Treatment Options -- PIPJ Arthroplasty without Fixation -- PIPJ Interpositional Arthroplasty -- PIPJ Arthroplasty with Temporary Kirchner Wire Fixation -- PIPJ Arthrodesis, Peg and Hole -- PIPJ Arthrodesis via Buried Kirchner Wire -- PIPJ Arthrodesis with a 1 Component Metallic Implant -- PIPJ Arthrodesis with a 2 Component Metallic Implant -- PIPJ Arthrodesis with a Thermal Activated Metallic Implant -- PIPJ Arthrodesis with an Allograft Implant -- PIPJ Arthrodesis with a Bioabsorbable Implant -- Flexor Tendon Transfer -- Tenotomies -- MTP Joint: Soft Tissue Balancing (skin plasties and lengthenings, MTPJ release, extensor tendon lengthening, capsulotomy, capsulorraphy) -- MTP Joint: Plantar Plate Reconstruction via a Dorsal Approach -- MTP Joint: Plantar Plate Repair via a Plantar Approach -- Lesser Metatarsal Osteotomies: uniplanar, biplanar wedge osteotomies -- MTP Joint: Transverse Plane Correction via Collateral Ligament Repair -- MTP Joint Transverse Plane Correction via Metatarsal Osteotomy -- Crossover Toe Correction -- Congenital Overlapping Fifth Toe -- Flail Hammertoe Correction
    Abstract: Comprised exclusively of clinical cases demonstrating the various management strategies for hammertoes, this concise, practical casebook will provide foot and ankle surgeons with the best real-world strategies to properly treat these frequent deformities of the lower extremity and their complications. Beginning with a review of the relevant anatomy and biomechanics of the toe, each subsequent chapter includes case material with a unique clinical presentation, followed by a description of the diagnosis, assessment and management techniques used to treat it, as well as the case outcome, literature review and clinical pearls and pitfalls. Chapters included illustrate different surgical fixation techniques, including PIPJ arthroplasty and arthrodesis, tendon transfers and tenotomies, and osteotomies, as well as management strategies for complications. Pragmatic and reader-friendly, Hammertoes: A Case-Based Approach is an excellent resource for foot and ankle surgeons treating this common but frustrating condition
    Pages: XIII, 419 p. 511 illus., 427 illus. in color. : online resource.
    ISBN: 9783319165523
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  • 40
    Keywords: Biochemistry ; Bioinformatics ; Protein Structure ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 55 Years of the Rossmann Fold -- Advances in Protein Super-Secondary Structure Prediction and Application to Protein Structure Prediction -- Automated Family-Wide Annotation of Secondary Structure Elements -- Computational Prediction of Secondary and Supersecondary Structures from Protein Sequences -- StackSSSPred: A Stacking Based Prediction of Supersecondary Structure from Sequence -- Information-Theoretic Inference of an Optimal Dictionary of Protein Supersecondary Structures -- Secondary and Supersecondary Structure of Proteins as Coupling between Local and Local and Backbone-Electrostatic Interactions: A View through Cluster-Cumulant Scope -- Learning Organizations of Protein Energy Landscapes: An Application on Decoy Selection in Template-Free Protein Structure Prediction -- Exploring Protein Supersecondary Structure through Changes in Protein Folding, Stability, and Flexibility -- Protodomains: Symmetry Related Supersecondary Structures in Proteins and Self-Complementarity -- bab Super Secondary Motifs: Sequence, Structural Overview and Pursuit of Potential Autonomously Folding bab Sequences from (b/a)8/TIM Barrels -- Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for their Prediction and Characterization -- Stable Sub-Structures in Proteins and How to Find Them using Single-Molecule Force Spectroscopy -- Supersecondary Structures and Fragment Libraries -- Molecular Dynamics Simulations of Conformational Conversions in Transformer Proteins -- Sequence Pattern for Supersecondary Structure of Sandwich-Like Proteins -- Homology Searches using Supersecondary Structure Code -- Beyond Supersecondary Structure: Physics-Based Sequence Alignment -- Secondary and Supersecondary Structure of Proteins in Light of the Structure of Hydrophobic Cores -- Hands On: Using Tryptophan Fluorescence Spectroscopy to Study Protein Structure -- Structural Characterization of Membrane Protein Dimers
    Abstract: This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation
    Pages: XVIII, 438 p. 119 illus., 103 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991617
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  • 41
    Keywords: Pharmaceutical technology ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Using Functional Genetics in Haploid Cells for Drug Target Identification -- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- In Silico Target Druggability Assessment: From Structural to Systemic Approaches -- In Silico Drug-Target Profiling -- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration -- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery -- In Vivo Pharmacology Models for Cancer Target Research -- Use of CRISPR/Cas9 for the Modification of the Mouse Genome -- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction -- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology -- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry -- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers -- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles -- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples
    Abstract: This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies
    Pages: XIII, 318 p. 40 illus., 29 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991457
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  • 42
    Call number: TK7882:3
    Pages: xvi, 370 p. : ill.
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    TK7882:3 on loan
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  • 43
    Call number: QC21:14(4)
    Keywords: Physics ; Chemistry ; Biochemistry
    Pages: xvii, 516 p. : ill.
    Edition: 4.,aktualisierte Aufl.
    ISBN: 9783662582497
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    QC21:14(4) available
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  • 44
    Call number: ERW
    ISBN: 9783410264002
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    ERW in acquisition
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  • 45
    facet.materialart.
    Singapore : Springer
    Call number: QH324:49
    Keywords: Bioinformatics ; Medicine ; Statistics ; Biomedicine general ; Statistics for Life Sciences, Medicine, Health Sciences
    Description / Table of Contents: Part 1. BIOINFORMATICS FOR LIFE AND PERSONAL GENOME INTERPRETATION -- Chapter 1. Bioinformatics For Life -- Chapter 2. Next Generation Sequencing and Personal Genome Data Analysis -- Chapter 3. Personal Genome Data Analysis -- Chapter 4. Personal Genome Interpretation and Disease Risk Prediction -- Part 2. ADVANCED MICROARRAY DATA ANALYSIS -- Chapter 5. Advanced Microarray Data Analysis -- Chapter 6. Gene Expression Data Analysis -- Chapter 7. Gene Ontology and Biological Pathway-based Analysis -- Chapter 8. Gene-set Approaches and Prognostic Subgroup Prediction -- Chapter 9. MicroRNA Data Analysis -- Part 3. NETWORK BIOLOGY, SEQUENCE, PATHWAY AND ONTOLOGY INFORMATICS -- Chapter 10. Network Biology, Sequence, Pathway and Ontology Informatics -- Chapter 11. Motif and Regulatory Sequence Analysis -- Chapter 12. Molecular Pathways and Gene Ontology -- Chapter 13. Biological Network Analysis -- Part 4. SNPS, GWAS AND CNVS, INFORMATICS FOR GENOME VARIANTS -- Chapter 14. SNPs, GWAS, CNVs: Informatics for Human Genome Variations -- Chapter 15. SNP Data Analysis -- Chapter 16. GWAS Data Analysis -- Chapter 17. CNV Data Analysis -- Part 5. METAGENOME AND EPIGENOME, BASIC DATA ANALYSIS -- Chapter 18. Metagenome and Epigenome Data Analysis -- Chapter 19. Metagenome Data Analysis -- Chapter 20. Epigenome Databases and Tools -- Chapter 21. Epigenome Data Analysis -- Appendix A. BASIC PRACTICE USING R FOR DATA ANALYSIS -- Appendix B. APPLICATION PROGRAM FOR GENOME DATA ANALYSIS INSTALL GUIDE
    Abstract: This textbook describes recent advances in genomics and bioinformatics and provides numerous examples of genome data analysis that illustrate its relevance to real world problems and will improve the reader’s bioinformatics skills. Basic data preprocessing with normalization and filtering, primary pattern analysis, and machine learning algorithms using R and Python are demonstrated for gene-expression microarrays, genotyping microarrays, next-generation sequencing data, epigenomic data, and biological network and semantic analyses. In addition, detailed attention is devoted to integrative genomic data analysis, including multivariate data projection, gene-metabolic pathway mapping, automated biomolecular annotation, text mining of factual and literature databases, and integrated management of biomolecular databases. This textbook is primarily intended for life scientists, medical scientists, statisticians, data processing researchers, engineers, and other beginners in bioinformatics who are experiencing difficulty in approaching the field. However, it will also serve as a simple guideline for experts unfamiliar with the new, developing subfield of genomic analysis within bioinformatics
    Pages: xvi, 367 p. : ill.
    ISBN: 9789811319419
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  • 46
    Keywords: Neurosciences ; Neurobiology ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- What Would Happen if Human Lives Beyond Time? -- Neurophysiology of Visual Perception -- Biophysics of Vision -- Cortex, Insula and Interoception -- Interoceptive dysfunction -- The Proprioceptive System -- Immunoperception -- The Immunoperceptive System -- Extrasensory Perception: Concept, Types and Interpretation -- Extrasensory Perception: Concept and History -- Psychological Perspective on Extrasensory Perception -- Environmental Sensitivity to Extra-Sensory Stimulations and Human Evolution -- A Perspective on the Concept and Nature of the Sixth Sense -- The Sixth Sense: Is It a Chance? -- Fuzzy Sets: Application to the Sixth Sense -- Asymmetry: Extra Sparkle to the Sixth Sense? -- Synchronization Side of the Sixth Sense Story -- Follow Aura and Find the Sixth Sense -- An Evolutionary Perspective of the Sixth Sense -- The Sixth Sense: Symphony of Spooky Actions -- The Sixth Sense Organs: The Immune System -- The Sixth Sense Organs: The Heart -- The Sixth Sense Organs: The Gut -- The Sixth Sense Organs: The Eyes -- The Sixth Sense Organs: The Ears -- The Sixth Sense Organs: The Hands -- Stem Cells Have More Than Five Senses -- Learning The Sixth Sense -- Neurocircuits Underlying Intuitive Processing -- Gut Feelings in Practice -- The Manager’s Sixth Sense: An Art in Organizational, Educational, Moral, and Expert Thinking
    Abstract: The book does not aim to constitute definite edges of the sixth sense, but it is to say here that the sixth sense is the result of a break in our present property. The present property is produced from integration of five common senses by the neuro-immuno-endocrine system. Thereby, mental representations of multisensory integration that primarily occur in the brain can reach throughout the body, including the heart and gut. Unconscious thought through synchronization between spatially distant brain networks would help break our present property. It would be a felicific calculus for society people to peek around the forgotten, but real life, corners through which the book “Biophysics and Neurophysiology of the Sixth Sense” seeks to explore
    Pages: XI, 362 p. 13 illus., 3 illus. in color. : online resource.
    ISBN: 9783030106201
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  • 47
    Keywords: Toxicology ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Complexity of Biologics CMC Regulation -- Biopharmaceutics are Not Chemical Drugs -- An Effective CMC Strategy is Possible -- Challenge of Adventitious Agent Control -- Biopharmaceutical Source Materials -- Manufacturing of Biopharmaceutical APIs -- Manufacturing of the Drug Product -- Complex Process-Related Impurity Profiles -- Product Characterization is a Journey -- Priceless Potency (Therapeutic Activity) -- Quality Attributes of a Biopharmaceutical -- Designing the Stability Program -- The Art of Setting Specifications -- Demonstrating Product Comparability After Process Changes -- Invaluable CMC-Focused Meetings with Regulatory Authorities
    Abstract: This book since first published in 2004 has been a major resource providing insights and practical guidance for the CMC teams to develop an acceptable cost-effective, risk-based CMC regulatory compliance strategy for biopharmaceuticals (recombinant proteins, monoclonal antibodies, genetically engineered viruses and genetically engineered human cells) from early clinical stage development through market approval. The third edition of this book provides added coverage for the biosimilars, antibody drug conjugates (ADCs), bispecific antibodies, genetically engineered viruses, and genetically engineered cells. This third edition of the book also addresses the heightened pressure on CMC regulatory compliance timelines due to the introduction of expedited clinical pathways moving the clinical development closer to a seamless phase process (e.g., FDA Breakthrough Therapy designation, CBER Regenerative Medicine Advanced Therapy (RMAT) designation, EMA Priority Medicines (PRIME) designation). The Challenge of CMC Regulatory Compliance for Biopharmaceuticals is essential, practical information for all pharmaceutical development scientists, Manufacturing and Quality Unit staff, Regulatory Affairs personnel, and senior management involved in the manufacture of biopharmaceuticals
    Pages: XXVIII, 426 p. 59 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9783030137540
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  • 48
    Keywords: Neurochemistry ; Biomedical Engineering ; Neurochemistry ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: 1. Past, present and future of neuronal models in vitro -- 2. In vitro models of neural disorders -- 3. Neuronal cultures and nanomaterials -- 4. Advances in Human Stem Cell Derived Neuronal Cell Culturing and Analysis -- 5. Large scale, high-resolution microelectrode arrays for interrogation of neurons and networks -- 6. Multi-site intracellular recordings -- 7. From MEAs to MOAs: the next generation of bioelectronic interfaces for neuronal cultures -- 8. Scaling spike detection and sorting for next generation electrophysiology -- 9. Burst detection methods -- 10. Reconstruction of functional connectivity from multi-electrode recordings and calcium imaging -- 11. Open-source tools for processing and analysis of in vitro extracellular neuronal signals -- 12. Screening chemicals for potential neurotoxicity and developmental neurotoxicity using neural networks grown on MEAs -- 13. Long-term activity dynamics of single neurons and networks -- 14. Electrical and optical closed-loop systems
    Abstract: This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies
    Pages: XV, 387 p. 102 illus., 90 illus. in color. : online resource.
    ISBN: 9783030111359
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  • 49
    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 50
    Keywords: Cytology ; Mass spectrometry ; Monoclonal antibodies ; Cell Biology ; Mass spectrometry ; Antibodies ; Springer eBooks
    Description / Table of Contents: Setting up Mass Cytometry in a Shared Resource Lab Environment -- Acquisition, Processing, and Quality Control of Mass Cytometry Data -- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating -- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments -- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis -- Titration of Mass Cytometry Reagents -- Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry -- Automated Cell Processing for Mass Cytometry Experiments -- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry -- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry -- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry -- Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry -- Mass Cytometric Cell Cycle Analysis -- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry -- Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry -- Data-Driven Flow Cytometry Analysis -- Analysis of Mass Cytometry Data -- Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry -- Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples -- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery
    Abstract: This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 338 p. 83 illus., 70 illus. in color. : online resource.
    ISBN: 9781493994540
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  • 51
    Keywords: Nucleic Acids ; Genetics ; Nucleic Acid Chemistry ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs -- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine -- Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue -- Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA -- 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability -- Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids -- Synthesis of Nucleobase-Functionalized Morpholino Monomers -- Synthesis and Application of LKγT Peptide Nucleic Acids -- Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids -- Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site -- Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids -- Labeling Peptide Nucleic Acids with Indium-111 -- Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet -- Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates -- Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis -- FRET Assay for Ligands Targeting the Bacterial A-Site RNA -- The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons -- Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA -- Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation
    Abstract: This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors
    Pages: XI, 315 p. 95 illus., 41 illus. in color. : online resource.
    ISBN: 9781493992164
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    Philadelphia : Wolters Kluwer/Lippincott Williams & Wilkins Health
    Call number: QZ200:570(5)
    Keywords: Neoplasms / therapy
    Notes: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Pages: xiv, 807 pages : illustrations
    Edition: 5th edition.
    ISBN: 9781496344182
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    QZ200:570(5) available
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    New York : W.H. Freeman
    Call number: QR181:93(8)
    Keywords: Immunology ; Immune System ; Immune System ; Immunity
    Pages: xxviii, 805, [104] p. : ill. (some col.)
    Edition: 8th ed.
    ISBN: 9781319114701
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    QR181:93(8) available
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    New York, NY : Springer
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Molecular Dynamics Simulations of the SNARE Complex -- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory -- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates -- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion -- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins -- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly -- Studying Munc18:Syntaxin Interactions using Small-angle Scattering -- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion -- SNAP25 S-Guanylation and SNARE Complex Formation -- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion -- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery -- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery -- Determination of Sec18-Lipid Interactions by Liposome Binding Assay -- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1 -- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins -- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles -- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution -- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs -- A Cell-Fr