Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Books  (29,644)
Collection
  • 1
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Making the Case for Functional Proteomics -- Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors -- Two-dimensional 16-BAC/SDS polyacrylamide gel electrophoresis of mitochondrial membrane proteins -- Systematic Glycolytic Enzyme Activity Analysis from Human Serum with PEP Technology -- A protein decomplexation strategy in snake venom proteomics -- Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level -- A systematic analysis workflow for high-density customized protein microarrays in biomarker screening -- Metaproteomics study of the gut microbiome -- Double-One Dimensional Electrophoresis (D1-DE) Adapted for Immunoproteomics -- BioID: A proximity dependent labeling approach in proteomics study -- Functional application of snake venom proteomics in in vivo antivenom assessment -- Proteomic detection of carbohydrate-active enzymes (CAZymes) in microbial secretomes -- An Overview of Mass Spectrometry-based Methods for Functional Proteomics -- Functional proteomic analysis to characterize signaling crosstalks -- Identification of unexpected protein modifications by mass spectrometry based proteomics -- Label-free LC-MS/MS Strategy for Comprehensive Proteomic Profiling of Human Islets Collected using Laser Capture Microdissection from Frozen Pancreata -- Mass Spectrometry based targeted proteomics for cell and tissue analysis -- Metabolomic investigation of Staphylococcus aureus antibiotic susceptibility by liquid chromatography coupled to high resolution mass spectrometry -- Nuts and bolts of protein quantification by on-line trypsin digestion coupled LC-MS/MS analysis -- Proteases: Pivot Points in Functional Proteomics -- The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics -- Efficient Extraction and Digestion of Gluten Proteins -- Glycosylation profiling of tumor marker in plasma using bead-based immunoassay -- Protein-specific analysis of invertebrate glycoproteins -- The use of proteomics studies in identifying moonlighting proteins -- Two-dimensional biochemical purification for global proteomic analysis of macromolecular protein complexes -- A data analysis protocol for quantitative data-independent acquisition proteomics
    Abstract: This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed
    Pages: XII, 477 p. 89 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988143
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Kohlhammer
    Call number: ERW
    Edition: 3. Aufl.
    ISBN: 9783170221376
    Signatur Availability
    ERW in acquisition
    BibTip Others were also interested in ...
  • 3
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation -- Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates -- Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides -- Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis -- A Multipronged Method for Unveiling Subtle Structural-Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies -- High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1 -- Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases -- Assays for Light Chain Amyloidosis Formation and Cytotoxicity -- Monitoring Aggregate Clearance and Formation in Cell-Based Assays -- Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor -- Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation -- Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry -- Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells -- A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction -- Super-Resolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells -- Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry -- Methods to Screen Compounds against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics -- Early Stage Discovery and Validation of Pharmacological Chaperones for the Correction of Protein Misfolding Diseases -- Constructing Kinetically Controlled Denaturation Isotherms of Folded Proteins Using Denaturant-Pulse Chaperonin Binding -- In Vitro Prion Amplification Methodology for Inhibitor Screening -- SolubiS: Optimizing Protein Solubility by Minimal Point Mutations
    Abstract: This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections on cellular and proteostasis assays as well as assays for protein folding correction and recovery, combining methods such as thermal shift assays, in silico improvement of protein solubility, and compound screening, an important area of research as it may open avenues for therapeutic strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Misfolding Diseases: Methods and Protocols serves as an ideal guide for researchers seeking to advance our knowledge of protein conformational disorders
    Pages: XIII, 338 p. 77 illus., 58 illus. in color. : online resource.
    ISBN: 9781493988204
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Phytoplasmas: An Introduction -- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques -- Phytoplasma Transmission: Insect Rearing and Infection Protocols -- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors -- Symptoms of Phytoplasma Diseases -- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas -- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene -- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas -- Real-time PCR Protocol for Phytoplasma Detection and Quantification -- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves -- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas -- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas -- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification -- Quantitative Analysis with Droplet Digital PCR -- Rapid Sample Preparation and LAMP for Phytoplasma Detection -- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly -- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’ -- Transcriptomic Analyses of Phytoplasmas -- Transcriptomic Analyses of Phytoplasmas -- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study -- Collection of Phloem Sap in Phytoplasma-infected Plants -- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas -- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas -- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) -- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants -- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants
    Abstract: This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 362 p. 75 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988372
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: Oncology ; Neurosciences ; Cancer Research ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Introduction to Brain Tumor Stem Cells -- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells -- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines -- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors -- Detecting Stem Cell Marker Expression Using the NanoString nCounter System -- Flow Cytometric Analysis of Brain Tumor Stem Cells -- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells -- Differentiation of Brain Tumor Initiating Cells -- The Study of Brain Tumor Stem Cell Migration -- The Study of Brain Tumor Stem Cell Invasion -- Cell Cycle Dynamics in Glioma Cancer Stem Cells -- Embryonic Stem Cell Models of Human Brain Tumors -- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory -- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF -- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells -- In Vitro Assays for Screening Small Molecules -- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma -- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci -- In Vivo Murine Models of Brain Metastasis -- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain
    Abstract: This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer
    Pages: XII, 254 p. 40 illus., 34 illus. in color. : online resource.
    ISBN: 9781493988051
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Detecting Cellular Senescence in Reprogramming -- DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA) -- Reactive Oxygen Species Detection in Senescent Cells -- Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo -- Relative Human Telomere Length Quantification by Real-time PCR -- Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP) -- Measuring the Inflammasome in Oncogene-induced Senescence -- Alarmin Detection in Senescent Cells -- IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence -- Genotoxic Stress-induced Senescence -- A Multi-parametric Assay to Evaluate Senescent Cells -- A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids -- Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry -- Quantification of Autophagy during Senescence -- Quantification of Aneuploidy in Mammalian Systems -- Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis -- Mouse Models of Accelerated Cellular Senescence -- Methods to Quantify the NF-kB Pathway during Senescence
    Abstract: This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 253 p. 45 illus., 38 illus. in color. : online resource.
    ISBN: 9781493989317
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    Keywords: Medicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Traditional Prenatal Diagnosis: Past to Present -- Overview of Preimplantation Genetic Diagnosis (PGD): Historical Perspective and Future Direction -- Non-Invasive Approaches to Prenatal Diagnosis: Historical Perspective and Future Directions -- Molecular Testing for Preimplantation Genetic Diagnosis of Single Gene Disorders -- Detection of Aneuploidy and Unbalanced Rearrangements using Comparative Genomic Hybridization Microarrays -- Aneuploidy Screening using Next Generation Sequencing -- DNA Extraction from Various Types of Prenatal Specimens -- Assessment of Maternal Cell Contamination in Prenatal Samples by Quantitative Fluorescent PCR (QF-PCR) -- Rapid Prenatal Aneuploidy Screening by Fluorescence In Situ Hybridization (FISH) -- Prenatal Detection of Chromosome Aneuploidy by Quantitative Fluorescence PCR -- Multiplex Ligation-Dependent Probe Amplification (MLPA) for Prenatal Diagnosis of Common Aneuploidies -- Chromosomal Microarray Analysis using Array Comparative Genomic Hybridization on DNA from Amniotic Fluid and Chorionic Villus Sampling -- Prenatal Diagnosis using Chromosomal SNP Microarrays -- Rapid Detection of Fetal Mendelian Disorders: Thalassemia and Sickle Cell Syndromes -- Prenatal Diagnosis of Cystic Fibrosis -- Prenatal Diagnosis of Tay-Sachs Disease -- Next-Generation Sequencing of Prenatal Structural Chormosomal Rearrangements using Large-Insert Libraries -- Prenatal Diagnosis by Whole Exome Sequencing in Fetuses with Ultrasound Abnormalities -- Quad Screen Test, A Multiplexed Biomarker Assay for Prenatal Screening to Assess Birth Defects: The Columbia University Experience using the Beckman Access2 Immunoassay Analyzer and Benetech PRA -- Isolation of Cell-Free DNA from Maternal Plasma -- Noninvasive Detection of Fetal Aneuploidy using Next-Generation Sequencing -- Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis
    Abstract: This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this book are divided into three sections: preimplantation genetic testing, traditional prenatal testing, and non-invasive prenatal testing. This book covers topics such as molecular testing for preimplantation genetic diagnosis of single gene disorders; DNA extraction from various types of prenatal specimens; prenatal diagnosis of cystic fibrosis and Tay-Sachs disease; chromosomal SNP microarrays; and isolation of cell-free DNA from maternal plasma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Prenatal Diagnosis, Second Edition is a valuable resource for any researcher interested in reproducing these techniques in their clinical laboratories
    Pages: XI, 363 p. 35 illus., 28 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988891
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Keywords: Plant Ecology ; Anthropology ; Plant anatomy ; Plant physiology ; Plant Systematics/Taxonomy/Biogeography ; Plant Ecology ; Anthropology ; Plant Anatomy/Development ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Preface -- Overview -- Preparation of qualitative research -- Execution of the qualitative research -- Participant observation and Field Journal -- Audio and video recording techniques for ethnobiological research -- Discourse of the collective subject as a method for analysis of data in ethnobiological research -- Qualitative data analysis -- The meta-analytical and macroecological approach of Ethnobiology -- Collection and analysis of risks associated with environmental changes -- Sensitivity to harvesting and local risk indexes in ethnobotanical research -- The use of multivariate tools in the study of traditional resource management systems -- Multidimensional analysis for testing conservation, ecological and ethnobiological hypotheses -- Ethnoecology in pluricultural contexts -- Ethnobotany and ethnoecology applied to historical ecology -- Biocultural collections -- Accessing the traditional knowledge on the use of cryptogams -- Ethical considerations and protocols in ethnoecological and ethnobiological research -- Challenges in ethnozoological research -- Methodology applied to geographical indication -- Recent advances in research methods and techniques -- Chemical profile and antioxidant activity of natural products.-Collection and extraction of fatty acids from animals and their possible applications in ethnopharmacological research -- Advanced methods in the chemical analysis of medicinal plants -- Population ecology of plant species submitted to extractivism -- Methods for measuring hunting pressure -- Rapid sampling techniques for vertebrate fauna -- Techniques for analyzing the sustainability of hunting -- Description of technological processes for optimization and enhancement of local uses -- Index
    Abstract: Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There have been, however, many advances in research as a result of interaction with different disciplines, but also due to more recent results, new original and interesting questions. This handbook provides the current state of the art methods and techniques in ethnobiology and ethnoecology, and related fields. This new volume, besides bringing new and original aspects of what is found in the literature, fills some of the gaps in volume one by including the most systematic and extensive treatment of methods and techniques in qualitative research. Along with the various methods covered in the individual chapters, the handbook also includes an extensive bibliography that details the current literature in the field
    Pages: XVI, 342 p. 42 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493989195
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: How It All Began: A Personal History of Gel-Electrophoresis -- Introduction to Protein Electrophoresis -- Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation -- Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis -- The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis -- Cellulose-Acetate Electrophoresis of Hemoglobin -- Native Polyacrylamide Gels -- Isoelectric Focusing on Non-Denaturing Gels -- Determination of protein Molecular weights on SDS-PAGE -- Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE -- Cationic Electrophoresis -- Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients -- SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins -- Tricine-SDS-PAGE -- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis -- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies -- Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-Agarose -- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis -- Two-Dimensional Difference Gel Electrophoresis -- Immunoelectrophoresis: A Method with Many Faces -- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular Mass -- Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide Bonds -- Identification of Proteins on Archived 2D Gels -- Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing -- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols -- Characterization of New Cyclic D, L – α – Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry -- “Microchip Electrophoresis”, with Respect to “Profiling of Aß Peptides in the Cerebrospinal Fluid of Patients with Alzheier’s Disease” -- Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift -- Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down Assay -- Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes -- Applications of Immobilized Metal affinity Electrophoresis -- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids -- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples -- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting -- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization -- Cell Surface Protein Biotinylation for SDS-PAGE Analysis -- Isolation of Proteins from Polyacrylamide Gels -- Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures -- Protein Extraction from Gels: A Brief Review -- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome -- Ultra-Rapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis -- A Brief Review of Other Notable Electrophoretic Methods -- Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit -- Artifacts and Common Errors in Protein Gel Electrophoresis
    Abstract: This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field
    Pages: XV, 523 p. 112 illus., 61 illus. in color. : online resource.
    ISBN: 9781493987931
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Arthropod Genome Sequencing and Assembly Strategies -- Genome Size Estimation and Quantitative Cytogenetics in Insects -- Isolation of HMW DNA from Insects Chapter -- Long range sequencing and assembly QC -- Integrated modeling of structural genes using MCuNovo -- Using BUSCO to Assess Insect Genomic Resources -- The GFF3toolkit—QC and Merge Pipeline for Genome Annotation -- Using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) to identify functional regulatory DNA in insect genomes -- Using RAMPAGE to identify and annotate promoters in insect genomes -- CRM Beyond Model Insects -- Whole-genome bisulfite sequencing for the methylation analysis of insect genomes -- Bioinformatic analysis of methylation patterns using bisulfite sequencing data -- Physical Genome Mapping Using Fluorescence in Situ Hybridization with Mosquito Chromosomes -- Target enriched RAD sequencing (TEEseq): A new high-throughput sequencing approach applied to the comprehensive characterization of endosymbionts
    Abstract: This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field
    Pages: XI, 237 p. 34 illus., 23 illus. in color. : online resource.
    ISBN: 9781493987757
    Signatur Availability
    BibTip Others were also interested in ...
  • 11
    Keywords: Medicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs) -- In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells -- Cell-based Gene Therapy System for Delivering BMPs -- Generation of Endogenous BMP Transcriptional Reporter Cells through CRISPR/Cas9 Genome Editing -- High-Throughput, Biosensor-based Approach to Examine Bone Morphogenetic Protein (BMP) – Receptor Interactions -- Mutagenesis and Imaging studies of BMP Signaling Mechanisms in C. elegans -- Gene Regulation of BMP Ligands in Drosophila -- Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway -- Proteolytic Activation of Bmps: Analysis of Cleavage in Xenopus Oocytes and Embryos -- Imaging and quantification of P-Smad1/5 in zebrafish blastula and gastrula embryos -- An Adult Zebrafish Model of Fibrodysplasia Ossificans Progressiva -- Generation and Identification of Genetically Modified Mice for BMP Receptors -- Phenotypic Analyses of Genetically Modified Mice for BMP Receptors -- Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-embedded Tissue Sections -- Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds -- Pharmacologic Strategies for Assaying BMP Signaling Function -- Bone Morphogenetic Proteins (BMPs) and Bone Regeneration -- Heterotopic Ossification in Mouse Models of Fibrodysplasia Ossificans Progressiva -- Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenografts
    Abstract: This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 265 p. 37 illus., 27 illus. in color. : online resource.
    ISBN: 9781493989041
    Signatur Availability
    BibTip Others were also interested in ...
  • 12
    Call number: QR181:93(8)
    Keywords: Immunology ; Immune System ; Immune System ; Immunity
    Pages: xxviii, 805, [104] p. : ill. (some col.)
    Edition: 8th ed.
    ISBN: 9781319114701
    Signatur Availability
    QR181:93(8) available
    BibTip Others were also interested in ...
  • 13
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Protocol to Compare Methods for Untargeted Metabolomics -- High Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry -- Measuring the Nutrient Metabolism of Adherent Cells in Culture -- 13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells -- Measuring In Vivo Tissue Metabolism using 13C Glucose Infusions in Mice -- Measuring Human Lipid Metabolism using Deuterium Labeling: In vivo and in vitro -- Measuring Rates of ATP Synthesis -- Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay -- Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells using Radioactive Tracers -- Determining Compartment Specific Metabolic Fluxes -- Determining the Impact of Metabolic Nutrients on Autophagy -- Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism -- Determining Macrophage Polarization upon Metabolic Perturbation -- Assessing the Impact of the Nutrient Microenvironment on The Metabolism of Effector CD8+ T Cells -- Development of Patient-derived Tumor Xenograft Models -- Imaging Glioma Progression by Intravital Microscopy -- Lipectomizing Mice for Applications in Metabolism -- Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants -- Determining the Intracellular Organization of Organelles -- The Fundamentals of Constructing and Interpreting Heat Maps
    Abstract: This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metabolic Signaling: Methods and Protocols aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level
    Pages: XII, 294 p. 67 illus., 53 illus. in color. : online resource.
    ISBN: 9781493987696
    Signatur Availability
    BibTip Others were also interested in ...
  • 14
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Molecular Dynamics Simulations of the SNARE Complex -- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory -- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates -- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion -- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins -- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly -- Studying Munc18:Syntaxin Interactions using Small-angle Scattering -- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion -- SNAP25 S-Guanylation and SNARE Complex Formation -- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion -- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery -- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery -- Determination of Sec18-Lipid Interactions by Liposome Binding Assay -- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1 -- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins -- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles -- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution -- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs -- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion -- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins -- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion -- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion -- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET and FCCS -- Visualization of SNARE-Mediated Organelle Membrane Hemifusion By Electron Microscopy -- Studies of the Secretory Machinery Dynamics by Total Internal Reflection Fluorescence Microscopy in Bovine Adrenal Chromaffin Cells -- Imaging SNAP-29 in Drosophila
    Abstract: This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field
    Pages: XIV, 407 p. 74 illus., 66 illus. in color. : online resource.
    ISBN: 9781493987603
    Signatur Availability
    BibTip Others were also interested in ...
  • 15
    Call number: Z070:500
    Pages: 547 p. : ill.
    Edition: 12., überarbeitete Auflage
    ISBN: 9783800748129
    Signatur Availability
    Z070:500 departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 16
    Call number: M280:1
    Keywords: Civil law / Germany ; Droit civil / Allemagne
    Pages: xxxiv, 3357 p.
    Edition: 78., neubearbeitete Aufl.
    ISBN: 9783406725005
    Signatur Availability
    M280:1 departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 17
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer -- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood -- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells -- Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo -- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors -- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance -- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity -- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro -- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency -- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity -- Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo -- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments -- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells -- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry -- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways -- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation -- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion -- Quantitative Identification of Senescent Cells in Cancer -- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance -- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment -- Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models -- A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers -- Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy -- Generation of CAR-T Cells for Cancer Immunotherapy
    Abstract: This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field
    Pages: XIII, 363 p. 76 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988853
    Signatur Availability
    BibTip Others were also interested in ...
  • 18
    Keywords: Plant breeding ; Agriculture ; Plant Breeding/Biotechnology ; Agriculture ; Springer eBooks
    Description / Table of Contents: An Introduction to Barley: The Crop and the Model -- Mutation Breeding in Barley: Historical Overview -- A Practical Guide to Barley Crossing -- Barley Anther Culture -- Isolated Microspore Culture in Barley -- TILLING in Barley -- Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings -- Agrobacterium-Mediated Transformation of Barley Immature Embryos -- Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses during Early Development -- Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy -- Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy -- Microarrays for High-Throughput Gene Expression Analysis of Barley -- Genome Engineering Using TALENs -- Creating Targeted Gene Knock-Outs in Barley Using CRISPR/Cas9 -- Genotyping-by-Sequencing on the Ion Torrent Platform in Barley -- DNA Methylation Analysis in Barley and Other Species with Large Genomes -- High Resolution RT-PCR Analysis of Alternative Barley Transcripts -- Exome Capture for Variant Discovery and Analysis in Barley
    Abstract: This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing
    Pages: XI, 313 p. 113 illus., 95 illus. in color. : online resource.
    ISBN: 9781493989447
    Signatur Availability
    BibTip Others were also interested in ...
  • 19
    Philadelphia : Wolters Kluwer/Lippincott Williams & Wilkins Health
    Call number: QZ200:570(5)
    Keywords: Neoplasms / therapy
    Notes: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Pages: xiv, 807 pages : illustrations
    Edition: 5th edition.
    ISBN: 9781496344182
    Signatur Availability
    QZ200:570(5) available
    BibTip Others were also interested in ...
  • 20
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Introduction to Drug Testing – The Expanding Role of Mass Spectrometry -- Quantification of Eight Cannabinoids Including Cannabidiol in Human Urine Via Liquid Chromatography Tandem Mass Spectrometry -- Analysis of Benzodiazepines for Drug Facilitated Assaults and Abuse Settings (Urine) -- Targeted Opioid Screening Assay for Pain Management Using High Resolution Mass Spectrometry -- Measurement of Buprenorphine and Norbuprenorphine in Urine -- Quantitation of Tapentadol by Liquid Chromatography – Tandem Mass Spectrometry -- Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS -- LC-MS/MS method for the quantification of the Leflunomide metabolite, Teriflunomide, in Human Serum/Plasma -- Analysis of Tryptic Peptides from Therapeutic Monoclonal Antibodies using LC-MS/MS -- Quantification of Methotrexate in Human Serum and Plasma by Liquid Chromatography Tandem Mass Spectrometry -- Simultaneous Determination of Tacrolimus and Cyclosporine A in Whole Blood by Ultra-Fast LC-MS/MS -- Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) Method to Quantify Gabapentin and Pregabalin in Urine -- The Evolving Landscape of Designer Drugs -- Analysis of Synthetic Cannabinoid Metabolites by Liquid Chromatography-Tandem Mass Spectrometry -- Quantification of Designer Opioids by Liquid Chromatography Tandem Mass Spectrometry -- Screening Analysis for Designer Stimulants by LC-MS/MS -- Drug Screening Using Liquid Chromatography Quadrupole Time of Flight (LC-QqTOF) Mass Spectrometry -- Alternate Matrices – Meconium, Cord Tissue, Hair, and Oral Fluid -- Salt-assisted Liquid-Liquid Extraction of Meconium for Analysis of Cocaine and Amphetamines by Liquid Chromatography-tandem Mass Spectrometry -- Detection of In Utero Cannabis Exposure in Umbilical Cord Tissue by A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method -- Quantitation of Ethyl β-D-glucuronide in Human Umbilical Cord Tissue by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) -- Analysis of Drugs in Oral Fluid using LC-MS/MS -- Determination of Cocaine and Metabolites in Dried Blood Spots by LC-MS/MS
    Abstract: This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusing on advances in technology, alternate matrices, and rapidly-changing classes of drugs of abuse, compounds pertinent to toxicology, and therapeutic agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, LC-MS in Drug Analysis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field
    Pages: X, 274 p. 36 illus., 24 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988235
    Signatur Availability
    BibTip Others were also interested in ...
  • 21
    Keywords: Medicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Identification and Analysis of Genes Associated with Inherited Retinal Diseases -- eQTL Analysis in Retinal Research -- Testing for known Retinal Degeneration Mutants in Mouse Strains -- CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo -- Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis -- Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells -- Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture -- Porcine RPE/Choroidal Explant Cultures -- The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells -- Cell Death Analysis in Retinal Cultures -- Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease -- Light Damage Models of Retinal Degeneration -- Induction and Readout of Oxygen-Iinduced Retinopathy -- Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9 -- Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases -- Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa -- Retinal Fundus Imaging in Mouse Models of Retinal Diseases -- Phenotyping of Mouse Models with OCT -- Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy -- Immuno-TEM/STEM in Retinal Research -- Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium -- Cell-based Therapy for Retinal Disease: The New Frontier -- In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy -- Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo -- Optimized Subretinal Injection Technique for Gene Therapy Approaches
    Abstract: This volume provides key updates on several of the first edition chapters and also includes new novel techniques, addressing the most recent technological developments and their applications in retinal research. Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field
    Pages: XIV, 417 p. 108 illus., 56 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493986699
    Signatur Availability
    BibTip Others were also interested in ...
  • 22
    Keywords: Biotechnology ; Human Genetics ; Biotechnology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Microinjection and Micromanipulation: A Historical Perspective -- Production of Transgenic Mice by Pronuclear Microinjection -- Transgene Recombineering in Bacterial Artificial Chromosomes -- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice -- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method -- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer -- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection -- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice -- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique -- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation -- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides -- Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection -- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications -- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm -- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line -- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology -- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos -- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells -- Generation of Rabbit Models by Gene Editing Nucleases -- Production of Genetically Engineered Porcine Embryos by Handmade Cloning -- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production -- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer -- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting -- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes -- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing -- Microinjection in Zebrafish for Genome Editing and Functional Studies -- Microinjection of Marine Fish Eggs -- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method -- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus -- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function
    Abstract: This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations
    Pages: XIV, 540 p. 134 illus., 96 illus. in color. : online resource.
    ISBN: 9781493988310
    Signatur Availability
    BibTip Others were also interested in ...
  • 23
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) -- Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells -- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD -- Chromatin Immunoprecipitation in Skeletal Myoblasts -- Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor -- Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation -- Lentivirus-Mediated RNAi in Skeletal Myogenesis -- Adipogenesis from Bovine Precursors -- Transcriptomic Profiling during Myogenesis -- Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle -- Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System -- LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue -- siRNA Gene Silencing during C2C12 Myogenesis -- Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury -- Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis -- Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis -- Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers -- Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis -- Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies -- RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis
    Abstract: This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis
    Pages: XII, 351 p. 84 illus., 64 illus. in color. : online resource.
    ISBN: 9781493988976
    Signatur Availability
    BibTip Others were also interested in ...
  • 24
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: 1. Isolation and identification of lactic acid bacteria from environmental samples -- 2. Isolation and identification of lactic acid bacteria from environmental samples -- 3. Isolation and identification of lactic acid bacteria from environmental samples -- 4. Isolation and identification of lactic acid bacteria from environmental samples -- 5. Isolation and identification of lactic acid bacteria from environmental samples -- 6. Isolation and identification of lactic acid bacteria from environmental samples -- 7. Isolation and identification of lactic acid bacteria from environmental samples -- 8. Isolation and identification of lactic acid bacteria from environmental samples -- 9. Isolation and identification of lactic acid bacteria from environmental samples -- 10. Isolation and identification of lactic acid bacteria from environmental samples -- 11. Isolation and identification of lactic acid bacteria from environmental samples -- 12. Isolation and identification of lactic acid bacteria from environmental samples -- 13. Isolation and identification of lactic acid bacteria from environmental samples -- 14. Isolation and identification of lactic acid bacteria from environmental samples -- 15. Isolation and identification of lactic acid bacteria from environmental samples -- 16. Isolation and identification of lactic acid bacteria from environmental samples
    Abstract: This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science
    Pages: X, 194 p. 58 illus., 10 illus. in color. : online resource.
    ISBN: 9781493989072
    Signatur Availability
    BibTip Others were also interested in ...
  • 25
    Keywords: Electrophoresis ; Biotechnology ; Electrophoresis ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Fabrication of Glass Microfluidic Devices -- Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices -- Sample Injection Techniques -- Sample Preconcentration Protocols in Microfluidic Electrophoresis -- Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection -- Micellar Electrokinetic Chromatography -- Microchip Isotachophoresis: Analysis of Pharmaceuticals -- Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination -- Nanochannel Gradient Separations -- Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications -- Band Broadening Theories in Capillary Electrophoresis -- Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation -- Microchip Electrophoresis Tools for the Analysis of Small Molecules -- Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out -- Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips -- Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow
    Abstract: This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals
    Pages: X, 255 p. 93 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989645
    Signatur Availability
    BibTip Others were also interested in ...
  • 26
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: General Considerations and Preoperative Work-up -- Periprosthetic Fractures about the Acetabulum with a Stable Cup -- Periprosthetic Fractures about the Acetabulum with a Loose Cup -- Periprosthetic Fractures of the Greater Trochanter and Proximal Femur with a Stable Prosthesis -- Periprosthetic Fractures of the Proximal Femur with a Loose Prosthesis -- Interprosthetic and Interimplant Fractures -- Periprosthetic Fractures of the Distal Femur with a Stable Prosthesis -- Periprosthetic Fractures of the Distal Femur with a Loose Prosthesis -- Periprosthetic Fractures of the Tibia with a Stable Prosthesis -- Periprosthetic Fractures of the Tibia with a Loose Prosthesis
    Abstract: This up-to-date, comprehensive yet concise guide for orthopedic surgeons covers the salient points of anatomy and operative approaches for the management of periprosthetic fractures of the hip and knee: breaks in bone that occur around the components or implants of a total joint replacement. The opening chapter focuses on the general considerations and work-up of these injuries, including epidemiology, classification, anatomy, and knowledge of the biomechanics and biology behind the treatments. The subsequent sections then discuss the hip and knee, respectively, and are case-based. These describe management strategies for the particular periprosthetic fracture with either a stable or a loose prosthesis as well as associated complications, including fractures of the acetabulum, the greater trochanter and proximal femur, distal femur, tibia, and interprosthetic and interimplant fractures. Written by experts in the field and replete with plentiful, practical tips and tricks, Periprosthetic Fractures of the Hip and Knee is a valuable resource for orthopedic and trauma surgeons, residents and fellows
    Pages: X, 161 p. 60 illus., 25 illus. in color. : online resource.
    ISBN: 9783319430089
    Signatur Availability
    BibTip Others were also interested in ...
  • 27
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: Microbial Metabolomics: A General Overview -- Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications -- Metabolomics: A Microbial Physiology and Metabolism Perspective -- Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry -- Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis -- Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis -- High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics -- Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry -- Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis -- Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS) -- Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli -- Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling -- Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis -- Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry -- A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism -- Cluster Analysis of Untargeted Metabolomic Experiments -- Machine Learning in Untargeted Metabolomics Experiments -- Dynamic 13C Labeling of Fast Turnover Metabolites for Analysis of Metabolic Fluxes and Metabolite Channeling -- Genome-Scale 13C Fluxomics Modeling for Metabolic Engineering of Saccharomyces cerevisiae
    Abstract: This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher
    Pages: X, 351 p. 83 illus., 50 illus. in color. : online resource.
    ISBN: 9781493987573
    Signatur Availability
    BibTip Others were also interested in ...
  • 28
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Introduction to FOXO Biology -- Characterization of microRNAs Regulating FOXO Expression -- Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter -- Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover -- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity -- Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes -- Characterization of FOXO Acetylation -- Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays -- Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes -- Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters -- RNAi Mediated Silencing of FoxO Factors -- Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO -- High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO -- Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus -- Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology -- Genome-wide Analysis for Identifying FOXO Protein Binding Sites -- Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors -- Following Transcriptome to Uncover FOXO Biological Functions -- Hydra as Model to Determine the Role of FoxO in Longevity -- Genetic Ablation of FOXO in Mice to Investigate its Physiological Role -- Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity -- Analysis of FOXO3a Gene Polymorphism Associated with Asthma
    Abstract: This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 270 p. 54 illus., 33 illus. in color. : online resource.
    ISBN: 9781493989003
    Signatur Availability
    BibTip Others were also interested in ...
  • 29
    Call number: QH447:220
    Pages: v, 126 pages : illustrations
    ISBN: 9780128163566
    Signatur Availability
    QH447:220 available
    BibTip Others were also interested in ...
  • 30
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Nitrogenases -- Bacteriochlorophyll and Coenzyme F430 -- Carbon Monoxide Dehydrogenases -- Molybdenum-Containing Enzymes -- Hydrogenases -- Genomic Manipulations of the Diazotroph Azotobacter vinelandii -- Purification of Nitrogenase Proteins -- Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase -- Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor -- Crystallization of Nitrogenase Proteins -- X-Ray Crystallography of Carbon Monoxide Dehydrogenases -- X-Ray Absorption Spectroscopy of Metalloproteins -- Electron Paramagnetic Resonance Spectroscopy of Metalloproteins -- Magnetic Circular Dichroism Spectroscopy of Metalloproteins -- Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site -- Computational Methods for Modeling Metalloproteins
    Abstract: This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems
    Pages: X, 275 p. 62 illus., 40 illus. in color. : online resource.
    ISBN: 9781493988648
    Signatur Availability
    BibTip Others were also interested in ...
  • 31
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Repurposing macromolecule delivery tools for plant genetic modification in the era of precision genome engineering -- The use of an automated platform to assemble multigenic constructs for plant transformation -- Ensifer-mediated transformation (emt) of rice (monocot) and oilseed rape (dicot) -- Setaria viridis as a model plant for functional genomic studies in C4 crops -- Transient transformation using particle bombardment for gene expression analysis -- Maize transformation using the morphogenic genes babyboom and wuschel2 -- Efficient and fast production of transgenic rice plants by agrobacterium-mediated transformation -- Protocol for Agrobacterium-mediated transformation and transgenic plant production of switchgrass -- Biolistic transformation of wheat -- Mesophyll protoplasts and PEG-mediated transfections: Transient assays and generation of stable transgenic canola plants -- A Unified Agrobacterium-mediated transformation protocol for alfalfa (Medicago sativa l.) and medicago truncatula -- Poplar transformation -- The genetic transformation of sweet orange (Citrus sinensis L. Osbeck) for enhanced resistance to citrus canker -- Genetic modification of grapevine embryogenic culture -- Agrobacterium-mediated transformation of Solanum tuberosum L., potato -- Agrobacterium tumefaciens-mediated transformation of tomato -- DNA break repair in plants and its application for genome engineering -- Gene stacking in plants through the application of site-specific recombination and nuclease activity -- CRISPR/Cas9 for mutagenesis in rice -- Plant biotechnology applications of zinc fingers technology -- Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture -- Developing transgenic agronomic traits for crops: targets, methods and challenges -- Transgenic and genome editing approaches for modifying plant oils -- Summary of molecular analysis methods for characterizing transgenic events -- Detection of transgenic proteins by immunoassays -- Systematic evaluation of field crop performance using modern phenotyping tools and techniques
    Abstract: This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security
    Pages: XIV, 444 p. 77 illus., 73 illus. in color. : online resource.
    ISBN: 9781493987788
    Signatur Availability
    BibTip Others were also interested in ...
  • 32
    Call number: Z070:501
    Pages: 2172 p.
    Edition: Stand: Januar 2019
    ISBN: 9783878417873
    Signatur Availability
    Z070:501 departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 33
    Call number: QZ269:201(3)
    Keywords: Neoplasms / diagnostic imaging ; Radiography
    Abstract: Known among radiation oncology residents as "the green book," Radiation Oncology: A Question-Based Review is a must-have learning tool for trainees and physicians who want to strengthen their long-term command of radiation oncology. The updated 3rd Edition reflects state-of-the-art advances in the field, with revised questions and answers, new professional guidelines, and new annual content updates for the eBook. Offers detailed questions on the natural history, epidemiology, diagnosis, staging, treatment recommendations, and treatment-related side effects for each cancer type. Contains a new primer section on immunotherapy. Retains popular features such as memory aids, quick-reference tables, and useful facts from other relevant disciplines such as radiology, anatomy, and medical oncology. Incorporates the latest ASTRO, AJCC, and NCCN guidelines, as well as seminal new studies and practice-changing trials, to reflect current radiation oncology practice. Includes new annual updates to the eBook for the life of the edition, with revised questions or answers that keep you current with new knowledge in the field. Ideal for both trainees and radiation oncologists as a high-yield introduction and review of the clinical management of major cancer types and conditions that are currently treated with radiation. Enrich Your eBook Reading Experience with Enhanced Video, Audio and Interactive Capabilities! Read directly on your preferred device(s), such as computer, tablet, or smartphone. Easily convert to audiobook, powering your content with natural language text-to-speech. Adapt for unique reading needs, supporting learning disabilities, visual/auditory impairments, second-language or literacy challenges, and more."--Provided by publisher
    Notes: Includes index.
    Pages: xxxi, 648 p.
    Edition: Third edition.
    ISBN: 9781496360366
    Signatur Availability
    QZ269:201(3) departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 34
    Call number: M010:72
    Pages: 177 p.
    Edition: 2. Aufl.
    ISBN: 9783648090466
    Signatur Availability
    M010:72 departmental collection or stack – please contact the library
    BibTip Others were also interested in ...
  • 35
    München : Elsevier
    Call number: QZ365WI320:139
    Keywords: Gastrointestinal system / Cancer ; Digestive System Neoplasms / therapy
    Notes: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Pages: xv, 569 p. : ill.
    Edition: 1. Aufl.
    ISBN: 9783437211522
    Signatur Availability
    QZ365WI320:139 available
    BibTip Others were also interested in ...
  • 36
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Protein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability ́€“ FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for Îł-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles.℗
    Abstract: This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.℗
    Pages: XI, 352 p. 82 illus., 63 illus. in color. : online resource.
    ISBN: 9781493973668
    Signatur Availability
    BibTip Others were also interested in ...
  • 37
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Two Dimensional Gel Electrophoresis and 2D-DIGE -- Comparative DIGE Proteomics -- 2D-DIGE and Fluorescence Image Analysis -- DIGE Analysis Software and Protein Identification Approaches -- Native DIGE: Efficient Tool to Elucidate Protein Interactomes -- Comparative Two-dimensional Fluorescence Gel Electrophoresis -- DIGE-based Phosphoproteomic Analysis -- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue -- Comparative 3-sample DIGE Analysis of Skeletal Muscles -- DIGE Analysis of ProteoMineŕ„Ø Fractionated Serum/Plasma Samples -- DIGE Analysis of Human Tissues -- DIGE Analysis of Animal Tissues -- Rapid 2D DIGE Proteomic Analysis of Mouse Liver -- Proteomic Analysis of Lung Tissue by DIGE -- Comparative Testis Tissue Proteomics using 2-dye versus 3-dye DIGE Analysis -- DIGE Analysis of Fish Tissues -- Protein Digestion for DIGE Analysis -- Subcellular Fractionation for DIGE-based Proteomics -- DIGE Analysis of Immunodepleted Plasma -- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays -- Enzyme Assay Methods to Validate DIGE Proteomics Data -- Immunoblot Analysis of DIGE-based Proteomics -- Immunofluorescence Microscopy for DIGE-based Proteomics.℗
    Abstract: This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters inDifference Gel Electrophoresis: Methods and Protocols℗ detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application℗ of℗ basic biological research, and applied biomarker discovery. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,℗ Difference Gel Electrophoresis: Methods and Protocols℗ aims to ensure successful results in the further study of this vital field
    Pages: X, 318 p. 44 illus., 24 illus. in color. : online resource.
    ISBN: 9781493972685
    Signatur Availability
    BibTip Others were also interested in ...
  • 38
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: The A-like Faker Assay for Measuring Yeast Chromosome III Stability -- The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast -- Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay -- Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitized Single-Stranded DNA Reporter System in Budding Yeast -- Analyzing Genome Rearrangements in Saccharomyces cerevisiaen: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"〉 -- High Resolution Mapping of Modified DNA Nucleobases using Excision Repair Enzymes -- Integrated Microarray-based Tools for Detection of Genomic DNA Damage and Repair Mechanisms -- Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics -- Inserting Site-specific DNA Lesions into Whole Genomes -- A qPCR-based Protocol to Quantify DSB Resection -- Alkaline Denaturing Southern Blot Analysis to Monitor Double-strand Break Processing -- Single Molecule Analysis of Resection Tracks -- Mapping DNA Breaks by Next-generation Sequencing -- Genome-wide Profiling of DNA Double-strand Breaks by the BLESS and BLISS Methods -- DNA Replication Profiling using Deep Sequencing -- Quantitative Bromodeoxyuridine Immunoprecipitation Analyzed by High-Throughput Sequencing (qBrdU-seq or QBU) -- Strand-specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast -- Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation -- Dynamic Architecture of Eukaryotic DNA Replication Forks in vivo, Visualized by Electron Microscopy -- A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation -- Single Cell Gel Electrophoresis for the Detection of Genomic Ribonucleotides -- Measuring the Levels of Ribonucleotides Embedded in Genomic DNA -- Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing -- Detection of DNA-RNA Hybrids in vivo -- Analysis of de novo Telomere Addition by Southern Blot -- Assays to Study Repair of Inducible DNA Double Strand Breaks at Telomeres -- Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent in situ Hybridization -- Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae -- Quantitative Analysis of the Rates for Repeat-mediated Genome Instability in a Yeast Experimental System -- Measuring Dynamic Behaviour of Trinucleotide Repeat Tracts in vivo in Saccharomyces cerevisiae -- The Detection and Analysis of Chromosome Fragile Sites -- Imaging of DNA Ultrafine Bridges in Budding Yeast -- Detection of Ultra-Fine Anaphase Bridges -- A Chromatin Fiber Analysis Pipeline to Model DNA Synthesis and Structures in Fission Yeast -- Long-term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy -- The CellClamper: A Convenient Microuidic Device for Time-Lapse Imaging of Yeast -- Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy -- Genome-wide Quantitative Fitness Analysis (QFA) of Yeast Cultures -- Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions -- Reporter-based Synthetic Genetic Array Analysis: a Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae -- Statistical Analysis and Quality Assessment of ChIP-seq Data with DROMPA -- Quantitative Analysis of DNA Damage Signaling Responses to Chemical and Genetic Perturbations
    Abstract: This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability, fluorescence microscopic techniques, and genomic and proteomic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genome Instability: Methods and Protocols aims to provide a comprehensive resource for the discovery and analysis of the proteins and pathways that are critical for stable maintenance of the genome
    Pages: XVI, 663 p. 133 illus., 100 illus. in color. : online resource.
    ISBN: 9781493973064
    Signatur Availability
    BibTip Others were also interested in ...
  • 39
    Keywords: Medicine ; Cancer Research ; Biomedicine ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics -- MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential -- Involvement of miRNAs and Pseudogenes in Cancer -- microRNAs Reprogram Tumor Immune Response -- APOBEC Gene Expression Regulated by microRNAs -- MicroRNAs Changes the Landscape of Cancer Resistance -- MicroRNA, Noise, and Gene Expression Regulation -- Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer -- Detection of Plasma microRNA Signature in Osteosarcoma Patients -- Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells -- Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy -- Evaluation of microRNA Delivery in vivo -- Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer -- Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data -- Construction of Multi-potent microRNA Sponge and its Functional Evaluation -- microRNA Sequencing Data Analysis Toolkits
    Abstract: This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition℗ aims to ensure successful results in the further study of this vital field
    Pages: XI, 222 p. 21 illus., 17 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493974351
    Signatur Availability
    BibTip Others were also interested in ...
  • 40
    Hoboken : Pearson Higher Education
    Call number: QH308:14(11)
    Keywords: Biology
    Notes: Previous edition: Campbell biology / Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, , Robert B. Jackson. Tenth edition. 2014.
    Pages: 1342, various pagings : ill.
    Edition: 11th edition.
    ISBN: 9781292170435
    Signatur Availability
    QH308:14(11) available
    BibTip Others were also interested in ...
  • 41
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.℗
    Pages: XII, 302 p. 66 illus., 63 illus. in color. : online resource.
    ISBN: 9781493974177
    Signatur Availability
    BibTip Others were also interested in ...
  • 42
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: An Overview of Recent Therapeutics Advances for Duchenne Muscular Dystrophy.-℗ Clinical Manifestations and Overall Management Strategies for Duchenne Muscular Dystrophy -- Cardiac Involvement in Duchenne Muscular Dystrophy and Related Dystrophinopathies -- Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry -- Imaging Analysis of the Neuromuscular Junction in Dystrophic Muscle -- System Biology Approach: Gene Network Analysis for Muscular Dystrophy -- Proteomic Profiling of the Dystrophin-Deficient Brain -- Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models -- Exon Skipping Therapy Using Phosphorodiamidate Morpholino Oligomers in the mdx52 Mouse Model of Duchenne Muscular Dystrophy -- Designing Effective Antisense Oligonucleotides for Exon Skipping -- Identification of Splicing Factors Involved in DMD Exon Skipping Events Using an In Vitro RNA Binding Assay -- The Use of Antisense Oligonucleotides for the Treatment of Duchenne Muscular Dystrophy -- PMO Delivery System℗ Using℗ Bubble Liposomes and Ultrasound Exposure℗ for Duchenne Muscular Dystrophy Treatment -- Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum / Plasma of Patients with Duchenne Muscular Dystrophy (DMD) -- Test of Anti-Fibrotic Drugs in a Cellular Model of Fibrosis Based on Muscle-Derived Fibroblasts from Duchenne Muscular Dystrophy Patients -- Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy -- Advanced Methods to Study the Cross-Talk Between Fibro-Adipogenic Progenitors and Muscle Stem Cells -- AAV6 Vector Production and Purification for Muscle Gene Therapy -- From gRNA Identification to the Restoration of Dystrophin Expression: A Dystrophin Gene Correction Strategy for Duchenne Muscular Dystrophy Mutations Using the CRISPR-Induced Deletion Method
    Abstract: This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on €œÓmicś€ techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.℗ Authoritative and practical, Duchenne Muscular Dystrophy: Methods and Protocols serves as a guide for researchers exploring the complicated nature of dystrophin in the hope of helping the victims of this disorder
    Pages: XII, 288 p. 49 illus., 34 illus. in color. : online resource.
    ISBN: 9781493973743
    Signatur Availability
    BibTip Others were also interested in ...
  • 43
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Biochemistry, general ; Springer eBooks
    Abstract: This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines
    Pages: XV, 512 p. 94 illus., 68 illus. in color. : online resource.
    ISBN: 9781493973989
    Signatur Availability
    BibTip Others were also interested in ...
  • 44
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: Emerging concepts in innate immunity -- Bioinformatic Assessment of macrophage activation by the innate immune system -- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system -- Modeling primary human monocytes with the trans-differentiation cell line BLaER1 -- Measurement of NF-ÎðB activation in TLR-activated macrophages -- Biochemical isolation of the myddosome from murine macrophages -- Generation of innate immune reporter cell lines using retroviral transduction -- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER) -- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis -- Methods for detecting ASC and ASC speck formation -- Measuring innate immune responses to bacterial viability -- Methods to study cell swelling-induced inflammasome activation -- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy -- Quantitative proteomics of secreted proteins -- Simultaneous detection of cellular viability and interleukin-1Îø secretion from single cells by ELISpot -- Detection and quantification of MAV aggregation via confocal imaging.℗
    Abstract: This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ Innate Immune Activation: Methods and Protocols℗ aims to be a useful and informative reference tool for further study into this vital field
    Pages: XIII, 251 p. 54 illus., 40 illus. in color. : online resource.
    ISBN: 9781493975198
    Signatur Availability
    BibTip Others were also interested in ...
  • 45
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Targeted Deletion of Hsf1, 2 and 4 Genes in Mice -- Role of Heat Shock Factors in Stress-induced Transcription -- Monitoring of the Heat Shock Response with a Real-time Luciferase Reporter -- Quantitative Profiling of Chaperone/client Interactions with LUMIER Assay -- Measurement of chaperone-mediated effects on Polyglutamine Protein Aggregation by the Filter Trap Assay -- Fluorescent-linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors -- A High-throughput Screen for Inhibitors of the Hsp90-Chaperone Machine -- Primary Colorectal Cells Culture as a Translation Research Model -- Cell Death and Survival Assays -- Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro0pt;"〉 -- nt-size: 12.0ptProteomic Profiling of Hsp90 Inhibitors -- Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and its Interaction with Specific Client Polypeptides -- Nucleotide Exchange Factors for Hsp70 Chaperones -- Determination of Hsp90 Activity through Activation of Glucocorticoid Receptors in Yeast -- Bacterial Hsp90 ATPase Assays -- Detecting Post-translational Modifications of Hsp90 -- Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90) -- background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; font-size: 12pt; font-family: "Times New Roman", serif; color: rgb(33, 33, 33);"〉A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond -- Computational Modeling of the Hsp90 Interactions with Cochaperones and Small Molecule Inhibitors -- Computational Analysis of the Chaperone Interaction Networks -- Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer -- Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, using the cmHsp70.1 MonoclonalDetection and Analysis of Extracellular Hsp90 (eHsp90) -- Molecular Chaperone Receptors -- Creation of Recombinant Chaperone Vaccine using Large Heat Shock Protein for Antigen-targeted Cancer Immunotherapy -- A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells -- Hsp70: A Cancer Target Inside and Outside the Cell -- Evidence for Hsp90 Cochaperones in Regulating Hsp90 Function and Promoting Client Protein Folding -- Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors.n〉ttom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"〉
    Abstract: This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.℗ Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIX, 445 p. 69 illus., 36 illus. in color. : online resource.
    ISBN: 9781493974771
    Signatur Availability
    BibTip Others were also interested in ...
  • 46
    New York, NY : Springer New York
    Keywords: Life sciences ; Bioinformatics ; Life sciences ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Conceptual Challenges in the Theoretical Foundations of Systems Biology -- An Integrative Approach toward Biology, Organisms, and Cancer -- Conceptual Challenges of the Systemic Approach in Understanding Cell Differentiation -- A Primer on Mathematical Modeling in the Study of Organisms and their Parts -- The Search for Systeḿ€™s Parameters -- Inverse Problems in Systems Biology: A Critical Review -- Systems Biology Approach and Mathematical Modeling for Analyzing Phase-space Switch during Epithelial-mesenchymal Transition -- Parameters Estimation in Phase-space Landscape Reconstruction of Cell Fate; A Systems Biology Approach -- Complexity of Biochemical and Genetic Responses Reduced using Simple Theoretical Models -- Systems Biology Modelling of Non-linear Cancer Dynamics -- Endogenous Molecular-cellular Network Cancer Theory: A Systems Biology Approach -- A Network-based Integrative Workflow to Unravel Mechanisms Underlying Disease Progression -- Spatiotemporal Fluctuation Analysis of Molecular Diffusion Laws in Live-cell Membranes -- A Method for Cross-species Visualization and Analysis of RNA-sequence Data -- Multi Agent Simulations of Population Behavior (A Promising Tool for Systems Biology) -- Metabolomics: Challenges and Opportunities in Systems Biology Studies -- Systems Biology-driven Hypotheses Tested In Vivo: The Need to Advancing Molecular Imaging Tools.℗
    Abstract: This volume focuses on methodological aspects of systems biology in order to provide a new theoretical approach with a robust and tailored experimental support. Chapters detail mathematical modeling, methodological issues, modulation of the collective behavior, metabolic, dynamic profiling, and quantitative morphological studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Systems Biology aims to ensure successful results in the further study of this vital field
    Pages: XVII, 364 p. 102 illus., 83 illus. in color. : online resource.
    ISBN: 9781493974566
    Signatur Availability
    BibTip Others were also interested in ...
  • 47
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Abstract: This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practice aims to ensure successful results in the further study of this vital field.℗
    Pages: XI, 303 p. 57 illus., 34 illus. in color. : online resource.
    ISBN: 9781493973958
    Signatur Availability
    BibTip Others were also interested in ...
  • 48
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: 1. Neurotrophic Factors: An Overview.-℗ Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions -- Culture of Rodent Cortical, Hippocampal, and Striatal Neurons -- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum -- Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents -- Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays -- Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening -- Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents -- Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice -- Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation -- Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity -- Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair -- Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia -- Diolistic Labeling and Analysis of Dendritic Spines -- Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses -- Methods for Quantitative Analysis of Axonal Cargo Transport -- Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis -- Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay -- Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay.-℗ Rat Pancreatic Beta-Cell Culture -- Growth and Neurotrophic Factors in Embryonic Stem Cells -- Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications -- Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies -- Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid Îø-Peptide Toxicity -- An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid Îø-Peptide (1-42) -- Active Induction of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice -- A Model of Systemic Inflammation to Study Neuroinflammation -- Spared Nerve Injury as a Long-Lasting Model of Neuropathic Pain -- An In Vivo Compression Model of Spinal Cord Injury -- A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury -- Middle Cerebral Artery Occlusion by an Intraluminal Suture Method -- Primary Motor Neuron Culture to Promote Cellular Viability and Myelination -- Culture of Human Primary Bone Cells and Phenotype Assessment -- Recreating Intestinal Peristalsis in the Petri Dish -- Synthesis of Biocompatible Titanate Nanofibers for Effective Delivery of Neuroprotective Agents -- Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides
    Abstract: This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.℗ Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology
    Pages: XV, 458 p. 121 illus., 70 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493975716
    Signatur Availability
    BibTip Others were also interested in ...
  • 49
    New York, NY : Springer New York
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Abstract: This volume presents techniques and recently developments in extracellular recording. The chapters in this book explore topics such as mouse, rat and zebrafish models; extracellular recordings on human cognition; single-unit recordings and multi-electrode arrays; and recordings in perinatal and early developing mice. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Extracellular Recording Approaches is a valuable resource that provides readers with up-to-date applications and methods to study brain regions involved in sensory, motor, and cognitive functions
    Pages: XIII, 298 p. 74 illus., 62 illus. in color. : online resource.
    ISBN: 9781493975495
    Signatur Availability
    BibTip Others were also interested in ...
  • 50
    Call number: QZ365WP870:446(2)
    Keywords: Breast Diseases / radiography ; Diagnosis, Differential / Case Reports ; Mammography / Case Reports
    Pages: xiii, 183 p. : ill.
    Edition: 2., vollst. überarb. Aufl.
    ISBN: 9783110330151
    Signatur Availability
    QZ365WP870:446(2) available
    BibTip Others were also interested in ...
  • 51
    Keywords: Medicine ; Gene Expression ; Medical genetics ; Cell Biology ; Biomedicine ; Gene Function ; Gene Expression ; Cell Biology ; Springer eBooks
    Abstract: The three-dimensional organization of the DNA inside the eukaryotic cell nucleus has emerged a critical regulator of genome integrity and function. Increasing evidence indicates that nuclear pore complexes (NPCs), the large protein channels that connect the nucleus to the cytoplasm, play a critical role in the establishment and maintenance of chromatin organization and in the regulation of gene activity. These findings, which oppose the traditional view of NPCs as channels with only one: the facilitation of nucleocytoplasmic molecule exchange, have completely transformed our understanding of these structures. This book describes our current knowledge of the role of NPCs in genome organization and gene expression regulation. It starts by providing an overview of the different compartments and structures of the nucleus and how they contribute to organizing the genome, then moves to examine the direct roles of NPCs and their components in gene expression regulation in different organisms, and ends by describing the function of nuclear pores in the infection and genome integration of HIV, in DNA repair and telomere maintenance, and in the regulation of chromosome segregation and mitosis. This book provides an intellectual backdrop for anyone interested in understanding how the gatekeepers of the nucleus contribute to safeguarding the integrity and function of the eukaryotic genome.℗
    Pages: VIII, 240 p. 32 illus. in color. : online resource.
    ISBN: 9783319716145
    Signatur Availability
    BibTip Others were also interested in ...
  • 52
    Keywords: Life sciences ; Medicine ; Biostatistics ; Life sciences ; Biostatistics ; Medicine/Public Health, general ; Springer eBooks
    Abstract: This edition is a pretty complete textbook and tutorial for medical and health care students, as well as a recollection/update bench, and help desk for professionals.℗ Novel approaches already applied in published clinical research will be addressed: matrix analyses, alpha spending, gate keeping, kriging, interval censored regressions, causality regressions, canonical regressions, quasi-likelihood regressions, novel non-parametric regressions. Each chapter can be studied as a stand-alone, and covers one field in the fast growing world of regression analyses.℗ ℗ ℗ The authors, as professors in statistics and machine learning at European universities, are worried, that their students find regression-analyses harder℗ than any other methodology in statistics. This is serious, because almost all of the novel methodologies in current data mining and data analysis include elements of regression-analysis. It is the main incentive for writing this 28 chapter edition, consistent of ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ - 28 major fields of regression analysis, ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ - their condensed maths, ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ - their applications in medical and health research as published so far, ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ - step by step analyses for self-assessment, ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ ℗ - conclusion and reference sections. Traditional regression analysis is adequate for epidemiology, but lacks the precision required for clinical investigations. However, in the past two decades modern regression methods have proven to be much more precise. And so it is time, that a book described regression analyses for clinicians. The current edition is the first to do so. It is written for a non-mathematical readership. Self-assessment data-files are provided through Springer' s "Extras Online".℗ ℗ ℗ ℗
    Pages: XII, 426 p. 511 illus., 65 illus. in color. : online resource.
    ISBN: 9783319719375
    Signatur Availability
    BibTip Others were also interested in ...
  • 53
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Description / Table of Contents: The Diversity of Bacterial Chemosensing -- Transmembrane Signal Transduction in Bacterial Chemosensing -- Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay -- Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries -- A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients -- Visualizing Chemoattraction of Planktonic Cells to a Biofilm -- Labeling Bacterial Flagella with Fluorescent Dyes -- All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins -- Mutational Analysis of Binding Protein/Chemoreceptor Interactions -- In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway -- FRET Analysis of the Chemotaxis Pathway Response -- Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout -- Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation -- Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface -- Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA -- Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo -- Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis -- Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins -- Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation -- Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing -- Chemotaxis to Atypical Chemoattractants by Soil Bacteria -- Screening Chemoreceptoŕ€“Ligand Interactions by High-Throughput Thermal-Shift Assays -- High-Throughput Screening to Identify Chemoreceptor Ligands.-℗ Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance.-℗ Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors -- Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY -- Quantitative Modeling of Flagellar Motor-Mediated Adaptation -- Molecular Modeling of Chemoreceptor:Ligand Interactions -- Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors
    Abstract: This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.℗ Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world
    Pages: XIII, 389 p. 29 illus. in color. : online resource.
    ISBN: 9781493975778
    Signatur Availability
    BibTip Others were also interested in ...
  • 54
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Identification of Disease Susceptibility Alleles in the Next Generation Sequencing Era -- Induced Pluripotent Stem Cells in Disease Modeling and Gene Identification -- Development of Targeted Therapies Based on Gene Modification -- What can we Learn about Human Disease from the Nematode C. elegans? -- Microbiome Sequencing Methods for Studying Human Diseases -- The Emerging Role of Long Noncoding RNAs in Human Disease -- Identification of Disease-related Genes using a Genome-wide Association Study Approach -- Whole Genome Library Construction for Next Generation Sequencing -- Whole Exome Library Construction for Next Generation Sequencing -- Optimized Methodology for the Generation of RNA-sequencing Libraries from Low-input Starting Material: Enabling Analysis of Specialized Cell Types and Clinical Samples -- Using C1 to Generate Single-cell Full-length cDNA Libraries for mRNA Sequencing -- MiSeq: A Next Generation Sequencing Platform for Genomic Analysis -- Methods for CpG Methylation Array Profiling via Bisulfite Conversion -- miRNA Quantification Method using Quantitative Polymerase Chain Reaction in Conjunction with the Cq Method -- Lentiviral--mediated CRISPR--cas9 Gene Editing of Primary Human Airway Epithelial Cells -- RNA Interference to Knockdown Gene Expression -- Using Luciferase Reporter Assays to Identify Functional Variants at Disease-associated Loci -- Physiologic Interpretation of GWAS Signals for type 2 Diabetes -- Identification of Genes for Hereditary Hemochromatosis -- Identification of Driver Mutations in Rare Cancers: The Role of SMARCA4 in Small Cell Carcinoma of the Ovary, Hypercalcemic Type (SCCOHT) -- The Rise and Fall and Rise of Linkage Analysis as a Technique for Finding and Characterizing Inherited Influences on Disease Expression
    Abstract: This volume presents detailed laboratory procedures in an easy to follow format that can be carried out with success by investigators lacking previous exposure to a specific research method. Chapter guide readers through the application of molecular approaches to disease gene identification and overviews, ℗ and case studies are also presented. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ Disease Gene Identification: Methods and Protocols, Second Edition℗ aims to help with℗ the identification and characterization of many more disease-related genes and provide novel, and effective strategies for disease treatment and prevention
    Pages: X, 400 p. 63 illus., 50 illus. in color. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493974719
    Signatur Availability
    BibTip Others were also interested in ...
  • 55
    Keywords: Political science ; Political theory ; Elections ; Game theory ; Political Science and International Relations ; Electoral Politics ; Game theory ; Political theory ; Springer eBooks
    Description / Table of Contents: Chapter 1: ℗ Beyond the Voter's Paradox -- ℗ Chapter 2: A General Model of Strategic Voting -- ℗ Chapter 3: The Stability Analysis of Voting Games -- ℗ Chapter 4: Electoral Participation Bias and the Welfare State -- ℗ Chapter 5: Toward a Structural Theory of Turnout
    Abstract: This Brief uses game-theoretic analysis to debunk the turnout paradox and offers an alternative economic model to elucidate the patterns behind the socioeconomic bias in turnout. The author argues that the turnout paradox́€”the idea that rational, strategic actors would not vote in an election€”ís an overstated problem, and that, contrary to widespread belief, game-theoretic models of elections with highly realistic parameters are compatible with high turnout. The author applies the method of stability sets to the study of voting games so as to characterize the behavior of electoral turnout in response to the gam這s structural parameters. To illustrate the power and potential of this framework, the author then develops a politico-economic model that generates testable theories about the way in which the modern welfare state and redistribution of wealth can shape the patterns of biased turnout that exist in most democracies. By turning a classic problem of rational choice into a source of new methods of analysis this Brief allows game theory to intervene in relevant conversations about the political economy of electoral participation, creating an opportunity for formal methods to make a welcome contribution to the discipline. As such, this Brief will be of use to scholars and student of political science, economics, political economy, and public policy, especially those who work in the tradition of formal methods. ℗ ℗
    Pages: X, 93 p. 9 illus. : online resource.
    ISBN: 9783319739489
    Signatur Availability
    BibTip Others were also interested in ...
  • 56
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Origins, Technological Development and Applications of Peptidomics -- Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics -- Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry -- Mass Spectrometric Identification of Endogenous Peptides -- Bioinformatics for Prohormone and Neuropeptide Discovery -- Substrate Capture Assay using Inactive Oligopeptidases to Identify Novel Peptides -- Non-targeted Identification of d-amino Acid-containing Peptides through Enzymatic Screening, Chiral Amino Acid Analysis and LC-MS -- Quantitative Peptidomics: General Considerations -- Quantitative Peptidomics with Isotopic and Isobaric Tags -- Quantitative Peptidomics using Reductive Methylation of Amines -- Metabolic Labeling to Quantify Drosophila neuropeptides and Peptide Hormones -- Data Preprocessing, Visualization and Statistical Analyses of Non-targeted Peptidomics Data from MALDI-MS -- Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity -- Mass Spectrometry Based Immunopeptidomics for the Discovery of Cancer Neo-antigens -- Milk Peptidomics to Identify Functional Peptides and for Quality Control of Dairy Products -- Neuropeptidomic Analysis of Zebrafish Brain -- Identification, Quantitation, and Imaging of the Crustacean Peptidome -- Identification of Endogenous Neuropeptides in the Nematode C. elegans using Mass Spectrometry -- EndoProteoFASP as a Tool to Unveil the Peptidome-protease Profile: Application to Salivary Diagnostics -- Methodology for Urine Peptidome Analysis Based on Nano-HPLC coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry -- Identification of Components in Frog Skin Secretions with Therapeutic Potential as Anti-diabetic Agents -- High-Accuracy Mass Spectrometry Based Screening Method for the Discovery of Cysteine Containing Peptides in Animal Venoms and Toxins -- Analysis of the Snake Venom Peptidome -- Identification of Peptides in Spider Venom Using Mass Spectrometry -- Single Cell Peptidomics: Approach for Peptide Identification by N-Terminal Peptide Derivatization -- Peptidomic Identification of Cysteine-Rich Peptides℗ from Plants -- Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss -- The Bright Future of Peptidomics.℗
    Abstract: This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics:Methods and Strategies℗ pro