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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 10 (1987), S. 143-148 
    ISSN: 1573-5044
    Keywords: Brassica alboglabra ; in vitro regeneration ; micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Different vegetative parts of Brassica alboglabra seedlings and mature plants were used as explants in culture. A high frequency (60–100%) of shoot regeneration was obtained from hypocotyl explants, nodal stem segments, internodal segments and shoot apices cultured on Murashige-Skoog basal medium. Addition of 6-benzylaminopurine and kinetin increased the average number of shoots per explant. When detached and transferred to basal medium, the shoots readily developed roots. Regenerated plantlets could be successfully transplanted in soil.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1573-5044
    Keywords: soybean ; somatic embryogenesis ; hormones ; tissue culture ; embryo morphology ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryos were induced in cultures of immature soybean (Glycine max (L.) Merr) embryos, or isolated cotyledons on MS modified medium supplemented with NAA and 2,4-D, BAP and ABA. When NAA and 2,4-D were compared at similar concentrations (25 and 23 μM), 2,4-D produced larger number of somatic embryos, however, embryogenesis efficiency was improved in media containing NAA by using higher levels (100–150 μM) of the auxin. Somatic embryo morphology varied with auxin type: NAA-induced embryos more closely resembled zygotic embryos than did 2,4-D-induced embryos. Additions of BAP or ABA to auxin-containing media had either no effect or reduced embryo production, although ABA altered the morphology of 2,4-D-induced embryos. In media containing both NAA and 2,4-D, the latter was dominant in terms of embryo morphology. The effects of subculture frequency and of transfers between 2,4-D and NAA media were investigated: Subculture frequency influenced mainly the frequency of normal embryos, while preculture on 2,4-D increased subsequent embryogenesis efficiency on NAA medium but reduced the frequency of normal embryos.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 15
    ISSN: 1573-5044
    Keywords: Glycine ; soybean ; plant regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract More than 5000 cultures, from 30 accessions of six Glycine species, were established to assess the rôle of plant genotype in the response to an agar-solidified culture medium containing B5 salts and vitamins, 3% w/v sucrose, 1.1 mg 1−1 BAP and 0.005 mg 1−1 IBA, already known to induce shoot regeneration in callus of G. clandestina. Shoot initiation was obtained in a variety of explants from G. canescens, G. falcata, G. latrobeana and G. tomentella. With the exception of G. latrobeana, development of buds into shoots followed transfer to B5-based medium with 0.2 mg−1 BAP and 0.005 mg 1−1 IBA. Shoots readily produced roots in hormone-free half-strength B5 medium. In G. latrobeana, both extension and rooting occurred on this medium. Shoot regeneration was obtained in 12 of 30 accessions evaluated, but one accession of G. canescens, G1171, produced shoots and plantlets at a consistently higher frequency than other accessions, with plantlet recovery in more than 70% of the cultures. Bud formation in callus of G. canescens G1171 also occurred if BAP was replaced by 1.0 mg 1−1 kinetin, 2i-p or zeatin, albeit at a lower frequency.
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  • 16
    ISSN: 1573-5044
    Keywords: Trifolium spp. ; Trifolium repens ; morphogenesis ; selected genotypes ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphogenic responses within the genus Trifolium were investigated by culturing various explants from seedlings of 72 species. Seedlings from 32 species produced callus alone, 40 produced adventitious shoots and/or roots, of which 25 species produced only shoots and 7 species formed only roots. Seedlings within each species also varied in their response to culture. The section of these seedlings most likely to produce adventitious shoots was the original shoot with the remnants of the surrounding hypocotyl and cotyledons, followed by the excised cotyledons themselves. Inter- and intra-varietal variation was observed in T. repens. Genotypes that produced adventitious buds were selected and crossed. An improvement in the proportion of the population capable of morphogenesis was observed in one cultivar.
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  • 17
    ISSN: 1573-5044
    Keywords: benzyladenine ; competence ; Pinus ponderosa ; proteins ; SDS-PAGE ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The placement of Pinus ponderosa embryos on a benzyladenine (BA) supplemented medium induces the differentiation of two distinct morphological types of cotyledons. Cotyledons in contact with the medium (lower cotyledons) do not elongate, and rapidly respond to BA by forming multiple buds along their entire length. Cotyledons not in contact with the medium (upper cotyledons) elongate, do not form buds, and are similar both morphologically and in protein profiles to seedling cotyledons that do not respond to BA. Using sodium dodecyl sulfate polyacrylamide electrophoresis, protein differences between these two cotyledon types can be detected as early as one week. These protein differences include the reduced level of a 52,000 Kilodalton (52 Kd) peptide and the appearance of a 4 Kd peptide in the lower cotyledons. These peptide differences may prove useful as markers for tissue that will respond to BA.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 19
    ISSN: 1573-5044
    Keywords: Rumex acetosella ; R. acetosa ; organogenesis in vitro vegetative clones ; dioecious plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures were established from dioecious plant species Rumex acetosella and R. acetosa, using cotyledons, hypocotyls and stem tips of aseptically germinated seedlings as primary explants. Cultures were also established from male and female R. acetosella adult plants, starting from vegetative lateral buds. Cell division was induced using a high 2,4-D concentration, while bud induction and multiplication were stimulated on a medium with high BAP/IAA ratio. Cotyledon fragments of both species produced only rhizogenic calli. Hypocotyl-derived calli of R. acetosella produced buds, while those of R. acetosa showed no bud forming response under these conditions. Bud multiplication occurred in stem tip cultures of both species and in lateral bud cultures of R. acetosella. Calli derived from male plants produced more buds than those from female. Shoots were easily rooted using IBA, and plantlets were effectively transferred to soil. Flowering was not induced in culture. The sex of regenerated male and female plants was not altered by the culture conditions.
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  • 20
    ISSN: 1573-5044
    Keywords: minimum growth storage ; acclimation ; vitrification ; light ; medium ; Gelrite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Storage of in vitro shoot cultures under reduced temperatures was investigated for eight Australian native woody plant species. Survival of multiple-shoot cultures for up to 12 months was best at 10°C in the light. Darkness increased the extent of vitrification and lower temperatures reduced survival. Acclimation at an intermediate temperature following storage at 10°C was sometimes beneficial. Survival was greater on Gelrite-based medium compared with agar, particularly at 22°C.
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