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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Aerobiologia 12 (1996), S. 213-214 
    ISSN: 1573-3025
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-3025
    Keywords: Airborne pollen ; Kiev ; Pollen calendar ; Gravimetric sampling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This article presents the results of aeropalynological observations in Kiev, carried out with a gravimetric method, during January–October, 1994. The six most abundant pollen types were: Betulaceae (21%), Chenopodiaceae/Amaranthaceae (10%), Ambrosia (10%), Artemisia (9%) Pinaceae (8%) and Poaceae (6%). Seasonal fluctuations of the atmospheric presence of tree/shrub and herb/grass pollen during the period March–September, 1993 and 1994, are also shown.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-3025
    Keywords: aerobiology ; bracken ; spores ; Scotland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field studies of bracken during sporing haveindicated very high numbers of sporesreleased per cubic metre of air. Thecarcinogenic nature of bracken spores poses ahealth hazard to workers and visitors in areasof sporing strands. A retrospective study of bracken spores trappedon slides exposed during Augustand September over the ten year period, 1988–1997, on a rooftop site in Edinburgh,has added an urban dimension to the aerobiologyof bracken. Results have revealed, apart from 1989,consistently low counts. The spore samplerwas situated downwind approximately 1.6 km fromthe nearest sporing stand.A spore calendar, based on the ten years ofcumulative data, has been constructed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-3025
    Keywords: aerobiology ; pollen allergy ; ragweed ; RAST ; Austria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ragweed pollen have been monitored since 1976 inVienna. Since 1984, the outdoor patients of theallergy department of the ear-nose-and-throatUniversity Clinic underwent both Skin Prick Test andRAST/CAP test with a standard series of commoninhalant allergens, ragweed (Ambrosia elatiorL.) included. Both the ragweed counts and the number of positiveRAST results showed a significant increase by time.Furthermore, a clear correlation between the number ofairborne pollen and the percentage of positiveRAST/CAP results is evident.The immune-response in the Viennese population ofatopic subjects is dependent on the amount of inhaledpollen.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-3025
    Keywords: domestic‐mite allergy ; specific immunotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-3025
    Keywords: allergens ; birch ; determination ; ELISA ; fine particles ; pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allergens in fine particles may cause symptoms inallergic asthmatics. In order to assess the exposureof susceptible persons, a method to measure theallergen load in fine and coarse particles wasdeveloped. Aerosols are collected with a high-volume air samplerby multistage impaction. They are separated into fivesize classes, ranging from 〉10 μm to 〈1 μmand sampled on glass fibre filters. After sampling,filters are crushed into a fine powder using ahydraulic press. Allergens are then eluted on a shakerinto Tween-20-containing phosphate buffered saline.After microfiltration, the eluate is ready foranalysis with ELISA-techniques (Enzyme Linked ImmunoSorbens Assay). Two different methods are used for the analysis ofallergens: One is a sandwich-ELISA using monoclonalIgG-antibodies, the other is a competitive ELISA basedon polyclonal IgE-antibodies obtained from patientsallergic to birch pollen. Using the monoclonalantibodies information on the amount of one particularallergen (the major allergen Bet v1) is obtained. Onthe other hand the competitive ELISA using thepolyclonal IgE is much more sensitive and indicatesthe total birch pollen allergens. Data obtained duringspring 1998 show good correlation of pollen counts andallergen content in the coarse particle fractioncontaining intact pollen (〉10 μm). In smallersized fractions, the allergen load is often close tothe detection limit. When clearly detectable amountsof allergen are present, in the fine size fraction theallergen load shows only a weak correlation to thepollen counts and the allergen concentrations in thecoarse particle fraction.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Aerobiologia 16 (2000), S. 171-176 
    ISSN: 1573-3025
    Keywords: aerobiology ; gamma ; log-normal ; pollen ; Probability Density Function ; statistics ; Successive Random Dilution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most statistical analyses are done under the premisethat the population distribution of the phenomenaunder study is normally distributed. In order to findthe best probability distribution (also called theprobability density function) that should be appliedto aerobiological data (in order to find the mostadequate error probability to apply in any hypothesistesting), the whole airborne pollen data set of 11years in Montréal (comprising 4070 days of mainpollination periods) was subjected to a distributionmodel analysis. When considered as a time-series(i.e. keeping successive days on the X-axis) the PDFthat best represents the aerobiological data set isindeed the Normal distribution. However, when onlythe frequency of different atmospheric concentrationsis used (i.e. having a number of occurrences on theY-axis), as would be done for a site-specific insteadof a time-specific distribution, the PDF is notnormally distributed. The resulting distributionappears log-normal to the eye, but the resultingfrequency distribution is in fact only asymptoticallylog-normal. It is in fact a Gamma distribution G(β, a). The two parameters of the Gammadistribution are its shape parameter (β) and itsscale parameter (a). In our case, β was 0.08and α was 0.002. The PDF is right-skewed when definedover the positive axis 0 〈 X 〈 ∞. Althoughlog-normal distribution is the most used datatransformation for parametric statistical analyses inaerobiology, it seldom accurately describes the lowerend of the observations near the minimum detectablelimits. Better aerobiological inferences could beobtained by using a Gamma PDF for calculatingthreshold probabilities.
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  • 8
    ISSN: 1573-3025
    Keywords: aeromycology ; Burdwan ; India ; indoor ; outdoor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparative survey of airborne fungal spores in fiveindoor and five outdoor environments in Burdwan, WestBengal, India, was carried out for a period of twoyears using rotorod samplers and sedimentation plates(culture plate). A total of 29 spore types wereidentified, of which three were Phycomycetous (Mucor, Rhizopus, Syncephalastrum), one Ascomycetous(Chaetomium), one Basidiomycetous (Ganoderma) and the remainder were Fungi Imperfecti. The results revealed lowest count during summer andmaximum during the rainy season. Aspergilluswas quite abundant in all the environments surveyed. The predominance of Aspergillus, Curvularia,Alternaria, Cladosporium, Drechslera, Fusarium in allthe surveyed environments has been attributed to theirability to grow in various substrata. The occurrenceof Cladosporium in the winter months suggestthat it is sensitive to high temperature. Allspore types were common in both environmentsexcept Bispora, Cercospora, Papularia, Spegazzinia, Trichothecium in the outdoor sites. Acorrelation has been made between the volumetriccomposition of airspora and the incidence of seasonalmold allergy.
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  • 9
    ISSN: 1573-3025
    Keywords: allergens ; blotting inhibition ; Corylus avellana ; cross-reactivity ; IgE ; Ostrya carpinifolia ; 2D-PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the last few years Ostrya carpinifolia pollen is consideredas an important cause of respiratoryallergy in Mediterranean areas. The concentration ofthe pollen was measured over a period of fifteen yearsfrom 1981 to 1996 in an area around Genoa; the resultsof this study have clearly indicated an increasingtrend that correlate with persons sensitization. In this study we sought to define the immunochemical andbiochemical properties of hop-hornbean pollen. Soluble proteins extracted from Ostryacarpinifolia pollen and from the taxonomicallyrelated species Corylus Avellana, were analyzedby polyacrylamide gel electrophoresis (SDS-PAGE), byhorizontal isoelectrofocusing (IEF) and by twodimensions electrophoresis (2D-PAGE). Allergenicproteins were identified with sera of sensibilizedpatients and cross-reactivity was evaluated byimmunoblotting techniques. The electrophoreticanalysis showed a partial identity between theproteins from Ostrya and Corylus extracts. The immunoblotting assay, developed withhuman IgE from subjects allergic to hop-hornbeampollen, displayed the major IgE reactivity for acomponent with a molecular weight of 17 kDa expressedin both Ostrya and Corylus extracts. This reactivity is consistent with the presence ofBet v 1 that is described as the major pollen allergenin the Betulaceae and Corylaceae families. Sera fromsubjects allergic to Ostrya were then preadsorbed with recombinant Bet v 1 immobilized in the Pharmacia CAP System; a significant reduction ofthe IgE binding activity was observed after thetreatment. We therefore suggest that Bet v 1 couldbe one of the allergenic proteins present in theOstrya pollen possibly being responsible forcross-reactivity with other members of taxonomicallyrelated families.
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  • 10
    ISSN: 1573-3025
    Keywords: allergenic species ; Graminaceae ; northern Italy ; phenology ; pollen emission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The results of phenological observations in ageomorphologically differentiated area are shown.Phenological surveys were conducted on flowering andpollen emission of some common and widely diffusedGraminaceae species, using a 7-step key. By means ofmultiple regression analysis altitude,latitude, slope and exposure were identified as significantenvironmental factors in giving rise to thepollination: in particular with respect to altitude thephenological pattern showed a negative gradient below100 m of altitude and a positive one above.
    Type of Medium: Electronic Resource
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