Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: microarray ; DNA ; DNA microarray ; microarrays ; DNA MICROARRAYS ; RNA AMPLIFICATION ; BIOLOGICAL NETWORKS ; disease markers ; methylation changes ; non-coding rnas ; quantitative transcriptome
    Abstract: During the last few years, high throughput RNA profiling technologies have become nearly indispensable tools in biomedical research. Optimization strategies for the different technologies and bioinformatics tools to mine the wealth of data have developed rapidly to meet the researcher's need calling for more sophisticated clinical and pharmaceutical applications and a more integrated view of the cell's primary molecules DNA, RNAs and proteins. Integrative network analyses based on quantitative expression data, and the integration of data gained from genome analysis defining the relationships between the cell's transcriptome and proteome, are becoming the focus of current research. Key issues to resolve include method developments to optimize analysis of small amounts of tissue or cells and low-abundance messages, and minimize biological noise. DNA microarray technology, oligonucleotide-based microarrays in particular, holds much promise, but may still be largely unexploited. In the wake of experience gathered during expression profiling of human prostate tumors, tissues from human infectious disease models and experimental rodent systems, we discuss here a number of novel DNA microarray-based approaches that may be used in the near future to conduct hypothesis-driven transcriptome research aiming at quantitative models of genetic networks and at narrowing the gap between the transcriptome and the proteome. Such research may also establish microarray technology as indispensable for clinical research near the patient's bedside.
    Type of Publication: Journal article published
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: CELLS ; EXPRESSION ; CELL ; Germany ; PATHWAY ; TOOL ; GENE ; GENOME ; microarray ; RNA ; COMPLEX ; COMPLEXES ; BIOLOGY ; MOUSE ; genetics ; MAMMALIAN-CELLS ; STRATEGIES ; MICROARRAY ANALYSIS ; review ; INTERFERENCE ; RNA INTERFERENCE ; SCIENCE ; methods ; HUMAN-CELLS ; SCREENS ; transcriptome ; FUNCTIONAL-ANALYSIS ; CANCER GENETICS ; Genetic ; STRATEGY ; ESSENTIAL GENES ; SHRNA LIBRARIES ; RNA-INTERFERENCE ; barcode ; half hairpin ; molecular tag ; Pooled RNAi screen ; SACCHAROMYCES-CEREVISIAE GENOME ; shRNA library
    Abstract: RNA interference (RNAi) screens have recently emerged as an exciting new tool for studying gene function in mammalian cells. In order to facilitate those studies, short hairpin RNA (shRNA) expression libraries covering the entire human transcriptome have become commercially available. To make use of the full potential of such large-scale shRNA libraries, microarray-based methods have been developed to analyze complex pooled RNAi screens. In terms of microarray analysis, different strategies have been pursued by different research groups, largely influenced by the employed shRNA library. In this review, we compare the three major shRNA expression libraries with a focus on their suitability for a microarray-based analysis of pooled screens. We analyze and compare approaches previously used to perform pooled RNAi screens and point out their advantages as well as limitations
    Type of Publication: Journal article published
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: IONIZING-RADIATION ; radiotherapy ; IN-SITU HYBRIDIZATION ; COMET ASSAY ; LYMPHOCYTES ; FISH ; RANDOMIZED-TRIAL ; radiosensitivity ; RECTAL-CANCER ; CHROMOSOME-ABERRATIONS
    Abstract: In radiotherapy the normal tissue reaction is often a limiting factor for radiation treatment. Still there is no screening method, which predicts normal tissue reaction on radiotherapy, especially in comparison to tumor tissue, and therefore allows tailoring of the radiation dose to each patient. Here, we present a case of severe radiation-related side effects. We applied classical cytogenetic techniques (Giemsa-banding and staining of centromeric regions), the comet assay as well as multicolor fluorescence in situ hybridization on peripheral blood lymphocytes of this patient in order to determine the radio-sensitivity on the DNA level and to correlate these findings with the clinical outcome. Our investigations revealed abnormalities on chromosome 9, deficiencies in the DNA-repair capacity after radiation exposure and a high number of radiation induced chromosomal aberrations. A detected high amount of residual damage two or three hours after radiation exposure and repair as well as the high number of chromosomal aberrations (ChAs) suggests a correlation between repair capacity and radiation induced ChAs. We concluded that the detected abnormalities might serve as a genetic basis for the radio-sensitive phenotype of this patient. Taken together this report strengthens the idea that intensive DNA genomic analysis of individual patients can serve as the basis for more favourable treatment of cancer patients.
    Type of Publication: Journal article published
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: COMPARATIVE GENOMIC HYBRIDIZATION ; IN-SITU HYBRIDIZATION ; TRANSLOCATION ; CHRONIC MYELOGENOUS LEUKEMIA ; CHRONIC MYELOID-LEUKEMIA ; COPY NUMBER CHANGES ; SOLID TUMORS ; CYTOGENETIC ANALYSIS ; blast crisis ; C-ABL-PROTEINS
    Abstract: Unregulated proliferation of mainly myeloid bone marrow cells and genetic changes in the hematopoietic stem cell system are important features in Chronic Myeloid Leukemia (CML). In clinical diagnosis of CML, classical banding techniques, fluorescence in situ hybridization (FISH) probing for the Philadelphia chromosome (Ph) or polymerase chain reaction amplifying the fusion products of the BCR-ABL fusion are state of the art techniques. Nevertheless, the genome of CML patients harbors many more cytogenetic changes. These might be hidden in subpopulations due to clonal events or involved in extremely complex aberrations. To identify these additional changes, several cytogenetic and molecular genetic techniques could be applied. Nevertheless, it has been proposed that identifying these aberrations is time consuming and costly and since they cannot be converted into a benefit for the patients, the necessity to perform these investigations has been questioned. In the times where highly specialized medicine is advancing into several areas of cancer, this attitude needs to be reassessed. Therefore, we looked at the usefulness of a combination of different techniques to unravel the genetic changes in CML patients and to identify new chromosomal aberrations, which potentially can be correlated to different stages of the disease and the strength of therapy resistance. We are convinced that the combination of these techniques could be extremely useful in unraveling even the most complex karyotypes and in dissecting different clones contributing to the disease. We propose that by doing so, this would improve CML diagnostic and prognostic findings, especially with regard to CML resistance mechanisms and new therapeutic strategies.
    Type of Publication: Journal article published
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: imaging ; GENE ; gene transfer ; GENE-TRANSFER
    Type of Publication: Journal article published
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...