Springer Online Journal Archives 1860-2000
Abstract Various aminoglycoside antibiotics—tobramycin "analytical standard"; tobramycin, gentamycin, kanamycin, and amikacin, all containing preservatives added in pharmaceutical preparations; and streptomycin—without preservatives—yield in aqueous solutions a conductance peak when titrated against a heparin aqueous solution, indicating the formation of a charge transfer complex, which subsequently dissociates. At the volume ratio corresponding to the peak, a colloidal suspensoid forms which is possibly a micellar complex stabilized by the ions produced by the dissociation of the complex. The clinical significance of this interaction is suggested by preliminary microbiological tests. The possible effects of plasma protein binding and dissociation of the complexes here reported in tissues, however, were not studied. In the interaction with heparin, the aminoglycoside antibiotics appear to act as electron acceptors, though heparin is reported to act as an acceptor against chlorpromazine, which is well known to be a strong electron donor. There appears to be an interaction between nonpreserved tobramycin and the preservative added for the pharmaceutical preparation, which would explain the difference in titration behavior between the antibiotic alone and the antibiotic with preservative. Conductance titrations of heparin against penicillin and cloxacillin, which are not aminoglycosides, show no evidence of complexation or any other interaction. No suspensoid forms.
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