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  • 1
    Keywords: AB-INITIO ; CANCER ; CELLS ; carcinoma ; Germany ; MODEL ; DIAGNOSIS ; INFORMATION ; PROTEIN ; PROTEINS ; MARKER ; KERATINOCYTES ; SKIN ; ACID ; NUMBER ; REGION ; SURFACE ; SCATTERING ; LIVING CELLS ; Raman ; HaCaT ; HUMAN HEPATOCYTES ; TUMORIGENICITY ; development ; protein degradation ; BASAL-CELL CARCINOMA ; Genetic ; BRAIN-TISSUE ; Intrinsic ; A5RT3,passage number ; MICROSPECTROSCOPY
    Abstract: Raman scattering provides molecular information about biochemical differences between healthy and cancerous cells in a non-invasive and non-destructive fashion. We have monitored such changes for the human skin keratinocyte cell line HaCaT and its cancerogenic counterpart A5RT3 at 514.5 and 647 nm excitations, with either fixed-cell droplets or adherent fixed and living cells for repeated preparations over time in order to discriminate intrinsic characteristic changes. Cell droplets yielded average but rather reproducible information and helped to rapidly determine such changes. The Raman spectra show differences in the relative intensity ratios of the protein amide I band at 1656 cm(-1) and amide III bands around 1250 cm(-1) and of the phenylalanine ring mode at 1003.6 cm(-1) to the CH2 deformation band at 1448 cm(-1), which are considerably greater for HaCaT cells than A5RT3 cells. Interestingly, these observations were accompanied by severe and consistent changes in the amide III region and in the collagen marker region around 936 cm(-1), therefore providing an unambiguous evidence of protein degradation and changes in the essential amino acid phenylalanine and in the lipid components in tumorigenic A5RT3 cells. Ultimately, in light of these intrinsic changes, the present findings are consistent with the passage number of the non-tumorigenic HaCaT cells, because low pass HaCaT showed less pronounced alterations than high pass HaCaT, suggesting a correlation of tumorigenic transformation with primarily genetic instabilities in HaCaT cells. This work represents the first Raman spectroscopy discrimination of the skin carcinoma model cell lines, the non-tumorigenic HaCaT and the cancerous A5RT3 cells, addressing the importance of delineating nonspecific variations from intrinsic characteristic changes and giving a spectroscopic indication for the influence of the passage number of HaCaT cells on the tumorigenic development. Copyright (C) 2009 John Wiley & Sons, Ltd
    Type of Publication: Journal article published
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 51-53 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman excitation profiles (REPs) for bands of carotenoid and chlorophyll b (Chl b) from whole cells of green algae at low temperatures are presented. In addition to preventing thermal destruction of the samples during long-term irradiation, the use of a double channel surface scanning technique allowed the recording of the Raman spectrum of a non-resonating substance such as acetone suitable as an intensity reference at the same time. The measured REPs are compared with the absorption spectrum of whole cells at low temperatures.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 55-58 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Fluorescein and its derivatives fluoresceinamine and diiodofluoresceiniodoacetamide have been studied by surface enhanced resonance Raman spectroscopy (SERRS). Adsorption of the dyes at the silver/electrolyte interface requires attractive interactions between polar side groups (such as NH2, OH and COOH) with the metal as a prerequisite for Raman enhancement and fluorescence quenching. It was possible to characterize two prototropic forms of fluorescein and fluoresceinamine by their SERR spectra. Only vibrations of the chromophoric part (xanthene) of the molecule contribute to the measured spectra. Substituents in the phenyl ring influence only the ability of adsorption and the structure of the adsorbed molecules.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The rotational Raman spectra of C2D4, C2HD3 and CH2CD2 are presented with a resolution of ca 0.3 cm-1. A computer simulation has been carried out to reproduce the observed contours from literature values of the rotational constants. The molecular polarizability parameters are determined with ca 10% uncertainty by transfer of the C2H4 literature values. The assignment of the J sub-band heads is shown.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 45-49 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The influence of high electrostatic fields on Raman scattering has been studied. For fields approaching 108 V m-1 in polymers a contribution to the Raman intensity due to the non-linearity of the Raman polarizability could be demonstrated. These results are in agreement with theoretical considerations. The band position does not change within the achieved resolution of 0.1 cm-1 for fields up to 1.7 × 108 V m-1, as also expected from theoretical estimates. With pulsed fields of duration 〈 1 μs, field strengths exceeding the static breakdown strength could be applied. In this case, however, a very complex pre-breakdown behaviour was observed, influencing the Raman scattering in a manner not yet understood.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 65-69 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Molecular reorientation of liquid trimethylchlorosilane (TMC) has been studied by means of Raman band shape analysis for the temperatures 298, 246 and 218 K. Experimental correlation times were compared with NMR relaxation time measurements and with diffusional and hydrodynamic models. Information theory was used to predict C(1)(t) given C(2)(t).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 71-73 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A general theory of Raman scattering in piezoelectric slab waveguides is presented. If the anisotropy of the uniaxial waveguide material is sufficiently large, incident slab-guided waves can be scattered by polaritons of the upper branch of the polariton dispersion curve. The angular dependence ω2(θ) of the scattered-wave frequency has been calculated in detail, for both y- and z-cut Ti:LiNbO3 waveguides in the polariton frequency range ωp=900-5160 cm-1 (pump wavelength 4880 Å). The detection of weak proton vibrations within the strong polariton Raman spectrum of proton-exchanged Ti:LiNbO3 waveguides is discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Lithium rubidium sulphate, LiRbSO4 (LRS), undergoes a sequence of four phase transitions at 166, 185, 202 and 204°C. The phase between 202 and 204°C is incommensurate. Polarized phonon Raman spectra in the frequency region of 50-1200 cm-1 are presented to identify the external and internal vibrational modes at room temperature. The internal mode frequencies of the sulphate ions are presented in the temperature region from -150 to 230°C covering all the phase transitions. The total integrated areas of the ν1, ν2 and ν4 modes show an anomalous increase across the phase transitions. The frequencies of the symmetric stretching (ν1) and symmetric bending (ν2) modes do not show any changes at the phase transitions, but the width of the ν2 mode shows changes across the phase transitions. A small increase in the linewidth of the ν2 mode observed in the incommensurate phase is attributed to the influence of the incommensurate modulation wave. A DSC thermogram showed endothermic peaks during heating at all the phase transitions. The IR spectrum recorded at room temperature showed the expected Au and Bu internal modes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectroscopic (75 K) and X-ray crystal structure (135 K) data for Na2Se·9(H, D)2O and Na2S·9(H, D)2O are compared, paying particular attention to the hydrogen bond networks in the two isostructural compounds. The crystal structures differ essentially only with respect to the hydrogen bond lengths of corresponding O—H…Se and O—H…S bonds, those to Se being uniformly larger by about 0.1 Å than those to S. The uncoupled νOD frequencies of isotopically dilute HOD molecules are very similar in both compounds, being larger only by about 10 cm-1 on average for the selenide than for the sulphide. It can therefore be claimed that the hydrogen-bond capabilities of the selenide and the sulphide ions are almost equal.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 17 (1986), S. 93-96 
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: It is shown that as a result of anharmonic coupling of the polariton waves, Stokes and anti-Stokes waves by a pump wave, an irradiated second wave (two-beam method) generates new waves from a slab. The frequency of the irradiated wave can be chosen from the Stokes and anti-Stokes visible region or from the polariton in the infrared region.First numerical results are given for GaP for reflectivity in the polariton and in the Stokes visible region. The spectra show strong amplification of the irradiated wave.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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