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  • 1
    Keywords: CELLS ; EXPRESSION ; tumor ; TUMOR-CELLS ; carcinoma ; PROTEIN ; MERKEL CELLS ; METASTASIS ; BREAST-CARCINOMA ; HUMAN-TUMOR-CELLS ; HOMOPHILIC BINDING ; INTEGRIN ALPHA(V)BETA(3) ; L1 ; MALIGNANT-MELANOMA ; NEURITE OUTGROWTH ; P-SELECTIN ; TRABECULAR CARCINOMA
    Abstract: Background: The neuroendocrine carcinoma of the skin is a rare malignant neuroendocrine tumor, which frequently metastasizes in regional lymph nodes or visceral organs. As adhesive interactions with endothelia, leukocytes, or thrombocytes enable malignant cells to penetrate the endothelium and to circulate in blood or lymphatic vessels, we here addressed the adhesion molecules CD171 (L1CAM) and CD24, which are known to be expressed by neurons, neuroblastomas, and other malignant tumors. Methods: Thirty-one neuroendocrine carcinomas of the skin (22 primary tumors, four recurrent tumors, and five metastases) were included in the study. Immunohistochemical staining of CD171 and CD24 was performed by the streptavidin- biotin-peroxidase-complex technique and a nickel-enhanced diaminobenzidine (DAB) reaction using the monoclonal antibodies UJ 127.11 and ML-5, respectively. Results: CD171 expression was detected in most neuroendocrine carcinomas of the skin, and staining was less frequent in metastases and recurrences in comparison with primary tumors which was statistically significant. The majority of neuroendocrine carcinomas of the skin was also positive for the mucin-like adhesion protein CD24. In contrast to tumor cells, cytokeratin 20-positive Merkel cells in 12 trichoblastomas and one fibroepithelioma of Pinkus were all negative for CD171 and CD24 staining. Conclusions: Expression of CD171 and CD24 is found in most neuroendocrine carcinomas of the skin, which may be used diagnostically. Further studies will assess whether this feature may contribute to metastasis of neuroendocrine carcinomas of the skin by facilitating transendothelial migration or tumor cell dissemination as has been suggested for other malignancies
    Type of Publication: Journal article published
    PubMed ID: 12834484
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  • 2
    Keywords: CANCER ; CELLS ; EXPRESSION ; proliferation ; radiotherapy ; tumor ; TUMOR-CELLS ; carcinoma ; CELL ; Germany ; human ; THERAPY ; DIAGNOSIS ; FOLLOW-UP ; DISTINCT ; TUMORS ; CONTRAST ; SKIN ; papillomavirus ; MEMBRANE ; p53 ; human papillomavirus ; RECURRENCE ; BENIGN ; HUMAN-PAPILLOMAVIRUS ; HUMAN-PAPILLOMAVIRUS DNA ; MORPHOLOGY ; SQUAMOUS-CELL CARCINOMA ; POLYMERASE-CHAIN-REACTION ; squamous cell carcinoma ; BEHAVIOR ; OROPHARYNGEAL ; ORAL CAVITY ; Bcl-2 ; MUCOSA ; DIFFERENTIAL-DIAGNOSIS ; EPITHELIOMA CUNICULATUM
    Abstract: Background: Verrucous carcinoma, a variant of squamous cell carcinoma, is distinct from squamous cell carcinoma in morphology and behavior. It preferentially occurs on the oropharyngeal mucosa, the urogenital mucosa, and the soles. In contrast to its malignant clinical picture, the tumor grows locally invasive but is histologically benign and metastasizes rarely.Methods: We report the uncommon occurrence of a large verrucous carcinoma on apparently uninvolved skin in the right axilla in a 47-year-old male.Results: Histologic examination reveals a cauliflower-like tumor consisting of deep invaginated epidermal proliferation with rabbit burrow-like, keratin-filled sinus formations; the basement membrane, however, remains intact. Immunohistology showed positivity for pancytokeratin (KL-1) and cytokeratin (CK) 18 and negativity for CK7, and assessment of the proliferative activity of the tumor cells revealed low percentage of Ki-67 expression. Furthermore, there were only scattered cells expressing p53 or bcl-2. Polymerase chain reaction excluded the presence of human papillomavirus. After complete excision, no signs of recurrence occurred over a follow-up period of three years.Conclusion: Verrucous carcinoma should be distinguished from typical squamous cell carcinoma. The clinicopathological features, differential diagnosis, and therapy are discussed here together with the molecular biologic aspects of the tumor
    Type of Publication: Journal article published
    PubMed ID: 14690468
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  • 3
    Keywords: CELLS ; EXPRESSION ; tumor ; carcinoma ; Germany ; neoplasms ; PATHWAY ; DIFFERENTIATION ; COMPLEX ; COMPLEXES ; TRANSCRIPTION FACTOR ; MEMBERS ; ASSOCIATION ; TRANSCRIPTION FACTORS ; NUMBER ; BENIGN ; FOLLICLE ; CATALOG ; MATRIX ; RE ; keratins ; LYMPH-NODE ; PULMONARY METASTASIS
    Abstract: Background: We have previously shown that benign pilomatricomas not only maintain the sequential expression of the hair matrix and precortex keratins hHa5 and hHa1 of normal hair follicles in their transitional cell compartment, but also preserve the association of hHa5 expression with that of its regulatory homeoprotein HOXC 13 in the lower transitional cell compartment. In contrast, hHa1 expression in the upper transitional cell compartment is uncoupled from the nuclear co-expression of the LEF1/beta-catenin complex seen in normal hair follicles (Cribier et al., J Invest Dermatol 2004; 122: 1078). Methods: Formalin-fixed paraffin sections of the tumor were examined using a panel of mono- and polyclonal hair and epithelial keratin antibodies as well as antibodies against HOXC 13, LEF1, and beta-catenin. Results: Morphologically, the malignant pilomatricoma investigated here clearly deviated from the described major tumor type by a large number of differently sized parakeratotic squamoid whorls emerging within the mass of basaloid cells and surrounded by cells remembering transitional cells, but only rarely containing shadow cells and signs of calcification. We show that hHa5/HOXC13 co-expression was maintained in transitional cell areas, in which hHa1 expression was much stronger than in benign pilomatricomas, but again uncoupled from concomitant nuclear LEF1/beta-catenin expression. Surprisingly, however, and in clear contrast to benign pilomatricomas, these transitional cells co-expressed the epithelial keratins K5, K14, and K17, with the latter being as strongly expressed as hHa1, both also staining the entire inner mass of the parakeratotic whorls. Conclusions: Although the malignant pilomatricoma investigated here was distinctive in that it contained a multitude of parakeratinizing whorls and no signs of calcification, it shared both hHa5/HOXC13 co-expression and disrupted hHa1/ beta-catenin-LEF1 expression in its transitional cell compartment around the whorls with benign pilomatricomas. However, in clear contrast to the latter, transitional cells of the malignant tumor also strongly expressed the epithelial keratins K5, K14, and K17. We speculate that the observed dominance of the epithelial differentiation pathway over the competing conventional shadow cell differentiation pathway may prevent massive calcification of the tumor
    Type of Publication: Journal article published
    PubMed ID: 16441405
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  • 4
    Keywords: MARKER ; MUTATIONS ; HYPERPLASIA ; ENTITY ; BASAL-CELL CARCINOMA ; APOCRINE MIXED TUMOR ; MELANOCYTIC MATRICOMA ; TDAG51 ; TRICHOEPITHELIOMA ; PILOMATRICOMAS
    Abstract: BACKGROUND: Pilomatricomas are tumors that emulate the differentiation of matrix cells of the hair follicle, showing cortical differentiation, with sequential expression of K35 and K31 keratins. Beta-catenin gene is frequently mutated in pilomatricoma, leading to beta-catenin nuclear accumulation, and to downstream expression of LEF1. Skin matrical tumors other than pilomatricoma are very rare, and comprise purely matrical tumors and focally matrical tumors. We aimed at studying cortical differentiation, beta-catenin pathway and expression of the follicular stem-cell marker PHLDA1 in a series of matrical tumors other than pilomatricoma. METHODS: In 36 prospectively collected tumors, K31, K35, CK17, LEF1, HOXC13, beta-catenin and PHLDA1 expressions were evaluated. Five pilomatricomas were used as controls. RESULTS: In 18 purely matrical tumors (11 matrical carcinomas, 4 melanocytic matricomas, 3 matricomas) and 18 focally matrical tumors (11 basal cell carcinomas, 3 trichoepithelioma/trichoblastomas, 4 others), sequential K35, HOXC13 and K31 expressions were found, indicating cortical differentiation. Germinative matrix cells were always CK17-, and showed nuclear beta-catenin accumulation, with LEF1 and PHLDA1 expressions. CONCLUSIONS: Nuclear beta-catenin and LEF1 expression was highly conserved in matrical tumors, and suggested a common tumorigenesis driven by Wnt pathway activation. PHLDA1 was consistently expressed in matrical tumors and in areas of matrical differentiation.
    Type of Publication: Journal article published
    PubMed ID: 24673383
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  • 5
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A reversed passive Arthus reaction was induced in guinea pigs using horseradish peroxidase as antigen. An electron microscopic study on the cutaneous localization of the immune complexes was performed applying a peroxidase reaction. Precipitates of immune complexes were found within the walls of small blood vessels and among the collagen bundles in the dermis. The adherence of immune complexes to numerous eosinophils was observed and some of immune complexes were phagocytosed by neutrophils. The adherence of immune complexes to fibroblasts and the deposits of immune complexes in some areas of the basement membrane zone, especially in the zona diffusa, were found in the upper dermis and in the papillae. In the lower layers of the epidermis, we observed immune complexes adhering to the cell membranes of keratinocytes.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A review is made of superficial fibrous tumors of childhood and differences between the pattern of fibrous tumor proliferation in children and adults are discussed. The fibrous tumors that occur specifically in childhood and infancy include recurring digital fibrous tumor, juvenile aponeurotic fibroma (cartilage analogue of fibromatosis), juvenile hyalin fibromatosis, fibrous hamartoma of infancy, congenital fibromatosis and fibromatosis colli (Torticollis). Palmoplantar fibromatosis and fibrosarcoma may appear in all ages. Dupuytren contracture, however, is not usually observed early in life. Similarly, the histiocytomas and dermatofibromas which are the most common fibrous tumors af adults are extremely rare in childhood.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In order to characterize the benign and malignant proliferation of lymphoid cells in skin, we compared surface markers and cytoplasmic organelles of cells in cutaneous lymphoid hyperplasia (CLH), lymphomatoid papulosis (LP), mycosis fungoides (MF), Sézary's syndrome (SS) and primary cutaneous malignant lymphoma (ML). The immunohistochemical study showed cells with both T- and B-cell markers in CLH, LP and early MF, whereas cells with only the T-cell marker were seen in late MF, SS and ML. T-cells in all cutaneous lesions possessed the surface marker common to T-cells of peripheral lymph nodes, and not that of central thymus cells. Cutaneous T-cells contained clustered or scattered dense core granules. Although no specific organelles indicative of benign or malignant lymphoid proliferation were found, there were several ultrastructural features that could help identifying each form of cutaneous lymphoid lesions. These included clustered or scattered dense-core granules, the variable degree of nuclear convolutions as well as dendritic arborization, and the presence or absence of 10 nm filaments.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The skin of a patient with giant axonal neuropathy was studied by light and electron microscopy. Enlarged melanocytes protruded into the papillary dermis. An increased number of microfilaments (100 A), which accumulated in rounded or irregular clusters was the main pathological feature. Melanosomes and melanisation were normal.A similar increase of microfilaments was seen in axons and Schwann cells of peripheral nerves, fibroblasts and endothelial cells. This suggests that this metabolic, probably inborn and genetic disorder, does not only affect the nervous system but also the whole microfilament system (cytoskeleton).
    Type of Medium: Electronic Resource
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