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  • Springer  (1,652,323)
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  • 1
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    Berlin : Springer
    Keywords: Toxicology ; Pharmacology
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-6053
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  • 2
    Keywords: Diagnosis, Computer-Assisted ; Diagnostic Imaging ; Image Processing, Computer-Assisted ; Robotics ; Surgery, Computer-Assisted ; Therapy, Computer-Assisted
    Notes: Latest issue consulted: 7th (Sept. 26-29, 2004).
    Pages: v. : ill.
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  • 3
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    Berlin : Springer
    Keywords: Biotechnology / methods
    Notes: This is a series title, single volumes see link below.
    ISSN: 1940-607X
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  • 4
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    Berlin : Springer
    Notes: This is a series title, single volumes see link below.
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  • 5
    Keywords: Microbiology ; Food science ; Microbiology ; Food science ; Springer eBooks
    Description / Table of Contents: Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products -- Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens -- The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens -- Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection -- Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices -- Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria -- EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes -- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions -- Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation -- Isolation of Bacterial RNA from Foods Inoculated with Pathogens -- The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress -- Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics -- Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells -- High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens -- Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae -- Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection -- Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback -- The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform -- Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics -- Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments -- Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars
    Abstract: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens
    Pages: XI, 278 p. 53 illus., 43 illus. in color. : online resource.
    ISBN: 9781493990009
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  • 6
    Keywords: Biochemistry ; Cell Membranes ; Biochemistry, general ; Membrane Biology ; Biological and Medical Physics, Biophysics ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- 1. Understanding Membranes and Vesicles: A Personal Recollection of the Last Two Decades -- 2. Advanced Concepts and Perspectives of Membrane Physics -- Part 2: Membranes by the Numbers -- 3. Membranes by the Numbers -- Part 3: Spatial Heterogeneities in Biomembranes -- 4. LIPID RAFTS – A PERSONAL ACCOUNT -- 5. Theories of Equilibrium In homogeneous Fluids -- 6. Critical lipidomics: The consequences of lipid miscibility in biological membranes -- 7. Lateral diffusion in heterogeneous cell membranes -- 8. Mechanical factors affecting the mobility of membrane proteins -- 9. Membrane domains under cellular recycling -- 10. Protein Pattern Formation -- Part 4: Biomembrane Mechanics and Consequences for their Functions -- 11. Biomembrane mechanical properties direct diverse cell functions -- 12. Spontaneous and intrinsic curvature of lipid membranes: back to the origins -- 13. Membrane-mediated interactions -- 14. Simulating protein-mediated membrane remodeling at multiple scales -- 15. Mechanosensitivity of membrane budding and tracking Lionel Foret -- 16. Common energetic and mechanical features of membrane fusion and fission machineries -- 17. Interaction of particles and pathogens with biological membranes -- 18. Adhesion of biological membranes -- 19. Spatial and mechanical aspects of signal transduction in the cell membrane -- 20. Protein-Induced morphological deformations of biomembranes -- 21. Fluctuations in active membranes
    Abstract: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike
    Pages: X, 623 p. 167 illus., 144 illus. in color. : online resource.
    ISBN: 9783030006303
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  • 7
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    Heidelberg : Springer
    Call number: QH442:222 ; ERW
    Keywords: Genetic Techniques
    ISBN: 9783642346934
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    QH442:222 available
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  • 8
    Keywords: Immunology ; Microbiology ; Medical virology ; Immunology ; Medical Microbiology ; Virology ; Springer eBooks
    Description / Table of Contents: Activity-based protein profiling – enabling multimodal functional studies of microbial communities -- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles -- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria -- Activity-Based Protein Profiling at the Host–Pathogen Interface -- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens -- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling -- ABPP and Host–Virus Interactions -- Activity-Based Protein Profiling for the Study of Parasite Biology -- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling -- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics -- Development of Activity-Based Proteomic Probes for Protein Citrullination -- Recent Advances in Activity-Based Protein Profiling of Proteases -- Opportunities for Lipid-Based Probes in the Field of Immunology -- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases -- Target Identification of Bioactive Covalently Acting Natural Products -- Applications of Reactive Cysteine Profiling
    Abstract: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions
    Pages: X, 417 p. 115 illus., 70 illus. in color. : online resource.
    ISBN: 9783030111434
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  • 9
    Keywords: Microbiology ; Biotechnology ; Biochemistry ; Microbial ecology ; Microbiology ; Applied Microbiology ; Environmental Engineering/Biotechnology ; Biochemistry, general ; Microbial ecology ; Springer eBooks
    Description / Table of Contents: AcetylCoA Synthesis and Regulation -- AcetylCoA Synthesis and Role in Bacteria -- Autophagy in Stationary Phase of Growth -- Bacterial Sphingolipids -- Cell-contact-dependent Outer Membrane Exchange in Myxobacteria -- Contributions of Membrane Lipids to Bacterial Cell Homeostasis Upon Osmotic Challenge -- Dynamic Membrane Structure: Function Analysis by Means of Chemical Probes -- Evolution of Hydrophobic Storage Polymers -- Evolution of Lipids -- Evolution of Membranes -- Fatty Acid Synthesis and Regulation -- Fatty Acids: Introduction -- Formation of Bacterial Glycerol-based Membrane Lipids: Pathways, Enzymes, Reactions -- Formation of Fatty Acids -- Formation of Hopanoids -- Formation of Isoprenoids -- Formation of Lipochitin Oligosaccharide Signaling Molecules -- Functional Roles of Individual Membrane Lipids in Bacteria, Archaea and Eukaryotic Microbes -- Functional Roles of Non-membrane Lipids in Bacterial Signaling -- Hopanoids and Membrane Integrity and pH Homeostasis -- Lipid A -- Lipid Chaperones -- Lipid Chaperones and Regulation -- Lipid Intermediates in Bacterial Peptidoglycan Biosynthesis -- Lipid Metabolism in Microalgae -- Lipid Rafts -- Lipid-protein Interactions -- Lipid-protein Interactions Determining Membrane Fluidity in Prokaryotes and Eukaryotes -- Lipids as Receptors -- Lipoteichoic Acid Synthesis and Function in Gram-positive Bacteria -- Membrane Disrupting Proteins -- Membrane Formation and Regulation -- Membrane Homeostasis Upon Nutrient (C, N, P) Limitation -- Membrane Homeostasis upon pH Challenge -- Membrane Lipid Biogenesis -- Membrane Structure:Function Analysis by Means of Chemical Probes -- Membrane Structure:Function Analysis Through Reconstitution In Vitro -- Membrane Vesicles, Nanopods and Nanotubes of Archaea -- Metabolism and Regulation of Glycerolipids in Yeast -- Metabolism and Roles of Sphingolipids in Yeast Saccharamyces cerevisiae -- Modeling Lipid Membranes -- Modeling Lipid Metabolism in Yeast -- Mycolic Acids: Structures, Biosynthesis , and Function -- Nonpolar Lipids in Yeast: Synthesis, Storage and Degradation -- Ornithine Lipids and Other Amino Acid-containing Acyloxyacyl Lipids -- Outer Membrane Extension Nanowires of Bacteria -- Outer Membrane Vesicles of Bacteria -- Phenolic Lipids Synthesized by Type III Polyketide Synthases -- Players in the Nonpolar Lipid Game - Proteins Involved in Nonpolar Lipid Metabolism in Yeast -- Polyhydroxyalkanoate Biogenesis -- Preface: Biogenesis of Fatty Acids, Lipids and Membranes -- Production of Wax Esters by Bacteria -- Protein Lipidation, Elucidation by Chemical Proteomics, and its Functional Roles -- Regulation of Membrane Lipid Homeostasis in Bacteria upon Temperature Change -- Role of Lipid Domains in Bacterial Cell Processes -- Role of Lipids in the Eukaryotic Secretory Pathway -- Role of the BAM Complex in Outer Membrane Assembly -- Structure:Function of Transmembrane Domains of Proteins and Transmembrane Organelles -- Synthesis of AcetylCoA from Carbon Dioxide in Acetogenic Bacteria -- Synthesis of Acetyl-CoA from Carbon Dioxide in Acetogenic Bacteria -- The Biosynthesis and Evolution of Archaeal Membranes and Ether Phospholipids -- Type III Polyketide Synthases Responsible for Phenolic Lipid Synthesis -- Vitamin Formation from Fatty Acid Precursors -- Wax Ester and Triacylglycerol Biosynthesis in Bacteria
    Abstract: Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes). All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes
    Pages: 161 illus., 115 illus. in color. eReference. : online resource.
    ISBN: 9783319504308
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  • 10
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Normal Growth in PKU Patients Under Low-Protein Diet in a Single-Center Cross-Sectional Study -- Serial Magnetic Resonance Imaging and 1H-Magnetic Resonance Spectroscopy in GABA Transaminase Deficiency: A Case Report.-Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment -- Cognitive and Behavioural Outcomes of Paediatric Liver Transplantation for Ornithine Transcarbamylase Deficiency -- Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation -- Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice -- Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period -- Severe Leukoencephalopathy with Clinical Recovery Caused by Recessive BOLA3 Mutations -- Neonatal Onset Interstitial Lung Disease as a Primary Presenting Manifestation of Mucopolysaccharidosis Type I -- A Middle Eastern Founder Mutation Expands the Genotypic and Phenotypic Spectrum of Mitochondrial MICU1 Deficiency: A Report of 13 Patients -- Disruption of the Responsible Gene in a Phosphoglucomutase 1 Deficiency Patient by Homozygous Chromosomal Inversion -- Evaluation of Disease Lesions in the Developing Canine MPS IIIA Brain -- Extrapolation of Variant Phase in Mitochondrial Short-Chain Enoyl-CoA Hydratase (ECHS1) Deficiency -- RFT1-CDG: Absence of Epilepsy and Deafness in Two Patients with Novel Pathogenic Variants -- Short-Term Administration of Mycophenolate Is Well-Tolerated in CLN3 Disease (Juvenile Neuronal Ceroid Lipofuscinosis)
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 35 illus., 20 illus. in color. : online resource.
    ISBN: 9783662586143
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  • 11
    Keywords: Proteomics ; Biochemistry ; Cell Membranes ; Medicine ; Proteomics ; Protein Science ; Membrane Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Part 1. Sterol specificity in modulating protein function: 1. Chirality effect on cholesterol modulation of protein function -- 2. A critical analysis of molecular mechanisms underlying membrane cholesterol sensitivity of GPCRs -- 3. Regulation of BK channel activity by cholesterol and its derivatives -- 4. Chiral specificity of cholesterol orientation within cholesterol binding sites in inwardly rectifying potassium channels -- Part 2. Indirect modulation of protein function by cholesterol: 5. Cholesterol effect on the physical properties of lipid membranes -- 6. Effect of cholesterol on the dipole potential of lipid membranes -- 7. Mass spectroscopy imaging of cholesterol -- 8. Cholesterol-dependent gating effects on ion channels
    Abstract: In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems
    Pages: X, 197 p. 45 illus., 33 illus. in color. : online resource.
    ISBN: 9783030042783
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  • 12
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels -- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling -- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators -- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes -- MCU Regulation in Lipid Bilayer and Electrophysiological Recording -- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer -- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter -- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana -- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes -- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes -- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle -- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis -- Ca2+-Dependent Regulation of TFEB and Lysosomal Function -- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum -- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2 -- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes -- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes -- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer -- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells -- Purification of Functional F-ATP Synthase from Blue Native PAGE
    Abstract: This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research
    Pages: XIII, 246 p. 48 illus., 39 illus. in color. : online resource.
    ISBN: 9781493990184
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  • 13
    Keywords: Plant breeding ; Agriculture ; Plant Breeding/Biotechnology ; Agriculture ; Springer eBooks
    Description / Table of Contents: Hybrid Sorghum Product Development and Production -- Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum -- Field Studies to Develop Weed Management Programs for Grain Sorghum -- Discovery of Sorghum Haploid Induction System -- Development of a Pedigreed Sorghum Mutant Library -- Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion -- Assaying Sorghum Nutritional Quality -- Tannin Analysis in Sorghum Grains -- Sorghum Phytochemicals and their Potential Impact on Human Health -- Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography -- Gene Editing in Sorghum through Agrobacterium -- Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment -- Novel Ternary Vectors for Efficient Sorghum Transformation -- Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa -- Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions -- Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case -- Background on Grain Sorghum Usage -- Assaying Sorghum for Fuel Production -- The Role of Sorghum in Renewables and Biofuels
    Abstract: This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum
    Pages: X, 281 p. 84 illus., 61 illus. in color. : online resource.
    ISBN: 9781493990399
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  • 14
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 15
    Keywords: Immunology ; Immunology ; Springer eBooks
    Description / Table of Contents: Isolation and Characterisation of Human Adipose-derived Stem Cells -- Isolation of Murine Hematopoietic Stem Cells -- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells -- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting -- Identification and isolation of Regulatory B cells in Mouse and Human -- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis -- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells -- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade -- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells -- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays -- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments -- Immune tolerance and rejection in organ transplantation -- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation -- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood -- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental -- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
    Abstract: This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 240 p. 43 illus., 36 illus. in color. : online resource.
    ISBN: 9781493989386
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  • 16
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Chapter 1. Chaperokine Activity of Heat Shock Protein -- Chapter 2. Extracellular HSP70, Neuroinflammation and Protection against Viral Virulence -- Chapter 3. The Biology of Extracellular HSP60 -- Chapter 4. Heat Shock Protein 60: A Mediator of Atherosclerosis and its Potential Therapeutic Role -- Chapter 5. Role of Heat Shock Protein 90 in Autoimmune Inflammatory Rheumatic Diseases -- Chapter 6. Upregulation of Cytoprotective Chaperones Mediate Better Tolerance to High Altitude -- Chapter 7. Role of Chaperone Mediated Autophagy in Viral Infections -- Chapter 8. Chaperones in Sterile Inflammation and Injury -- Chapter 9. Chaperone in Inflammation and Immunomodulation: Resistin and PPlases -- Chapter 10. Exercise-induced Chaperokine Activity of Hsp70: Possible Role in Chronic Diseases -- Chapter 11. Physical Exercise and Heat Shock Proteins -- Chapter 12. Extracellular Hsp90 Governs Spatio-temporal Regulation of Biological Responses -- Chapter 13. HSP47 and its Involvement in Fibrotic Disorders -- Chapter 14. Therapeutic Implications of Heat Shock Proteins in Cancer -- Chapter 15. Targeting Heat Shock Proteins for Immunotherapy -- Chapter 16. Modulation of Heat Shock Proteins by Natural Products
    Abstract: Chaperokine is a term that describes the unique function of extracellular heat shock protein (eHsp) as both chaperone and cytokine. The cellular consequence of binding and signaling of eHsp is the stimulation of a potent and long lasting immune response. eHsp induces a plethora of immune responses including the release of bioactive mediators such as cytokines, chemokines, nitric oxide, apotogenic mediators, stimulation of the innate and adaptive immune response, migration and maturation of dendritic cells (DC) and the enhancement of natural killer cell-mediated cellular cytotoxicity. This edited book provides the most comprehensive review of contemporary knowledge of the chaperokine activity of heat shock proteins (HSP) in biology and medicine. Using an integrative approach to understanding the chaperokine activity of HSP, the contributors provide an overview of novel mechanisms, signal transduction pathways and a concise understanding of how the principles of the chaperokine activity of HSP has been harnessed for therapeutic gain. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed chapters that review present cutting-edge research activities and importantly, the future direction of chaperokine research. The book is a must read for researchers, postdoctoral fellows and graduate students in the fields of Translational Medicine, Clinical Psychology, Human Physiology, Zoology, Botany, Biotechnology, Molecular Medicine, Infectious Disease, Pathology and Pharmaceutical Sciences, as well as for researchers involved in Drug Discovery
    Pages: IX, 320 p. 49 illus., 28 illus. in color. : online resource.
    ISBN: 9783030022549
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  • 17
    Keywords: Stem Cells ; Gene Therapy ; Oncology ; Gastroenterology ; Stem Cells ; Gene Therapy ; Cancer Research ; Gastroenterology ; Springer eBooks
    Description / Table of Contents: 1. Introduction -- 2. Stem cells principles and biology -- 3. The truth behind esophagus: the stem cells significance -- 4. Pancreatic Diseases: The Role of Stem Cells -- 5. Stem cells therapy for liver diseases -- 6. The role of stem cells in colorectal cancer carcinogenesis and treatment -- 7. THE ROLE OF STEM CELLS IN THE TREATMENT OF ANAL FISTULAS -- 8. Stem cells in inflammatory bowel disease: From pathogenesis to clinical practice -- 9. Paneth Cells Physiology and Pathophysiology in Inflammatory Bowel Disease
    Abstract: This book reviews recent knowledge of the role of stem cells in the gastrointestinal system. It covers extensive topics for each organ, including the pancreas, esophagus, liver, and colon, while also discussing the contributions of stem cells to therapeutic approaches toward gastrointestinal diseases, including inflammatory bowel diseases. Comprehensive and cutting-edge, Digestive System Diseases: Stem Cell Mechanisms and Therapies deepens a reader’s theoretical expertise in gastrointestinal stem cell biology. It furthers scientists’ understanding of gastrointestinal stem cells and, most importantly, the development of novel therapeutic targets. Graduate and postdoctoral students, medical doctors (including gastroenterologists and surgeons), and principal investigators in both academia and industry will benefit from this book. In particular, it is a valuable resource for professionals within the fields of gastrointestinal research, pharmaceutical science, molecular biology, regenerative medicine, and genetics
    Pages: XIII, 187 p. 16 illus., 15 illus. in color. : online resource.
    ISBN: 9783030119652
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  • 18
    Keywords: Medical genetics ; Medicine ; Biochemistry ; Neurosciences ; Gene Function ; Molecular Medicine ; Protein Science ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Chapter 1. Molecular and Functional Properties of Progranulin -- Chapter 2. Progranulin as a biomarker for neurodegenerative diseases -- Chapter 3. PGRN and FTLD -- Chapter 4. PGRN and neurodegenerative diseases other than FTLD -- Chapter 5. Progranulin Regulations of Lysosomal Homeostasis and its Involvement in Neurodegenerative Diseases -- Chapter 6. Molecular and Functional Properties of Progranulin -- Chapter 7. PGRN and neuroinflammation -- Chapter 8. Neural Stem/Progenitor Cells and Progranulin -- Chapter 9. Generation and phenotyping of progranulin-deficient mice -- Chapter 10. Pleiotropic protective effects of progranulin in the treatment of ischemic stroke -- Chapter 11. New therapeutic approaches against ocular diseases
    Abstract: This book presents the latest knowledge on the roles of progranulin (PGRN) in normal physiology and pathology and explores the emerging significance of PGRN as a therapeutic target and biomarker in various CNS disorders, including frontotemporal lobe degeneration and other neurodegenerative diseases. Following initial recognition of the importance of PGRN in sexual differentiation of the developing brain and adult neurogenesis, it was subsequently discovered that PGRN acts as a chaperone of lysosomal enzymes and plays a crucial role in maintaining cellular protein homeostasis. It has also been found that sex steroids modulate the expression of PGRN and its trophic effects in the developing CNS and that PGRN directly or indirectly influences neural stem and progenitor cells. Against this background, deeper understanding of the molecular and functional properties of PGRN would provide fresh impetus for the development of mechanism-based therapeutic approaches for multiple disorders. Medications targeting the recovery of lysosomal function appear to hold particular promise in patients with neurodegenerative diseases resulting from PGRN insufficiency. In examining multiple aspects of this fascinating field, the book will be of high value for researchers and graduate students
    Pages: VII, 183 p. 37 illus., 13 illus. in color. : online resource.
    ISBN: 9789811361869
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  • 19
    Keywords: Medicine ; Mathematical Statistics ; Biomedicine general ; Statistics and Computing/Statistics Programs ; Springer eBooks
    Description / Table of Contents: Preface -- General Introduction -- Significant and Insignificant Adverse Effect -- Incidence Ratios and Reporting Ratios of Adverse Effects -- Safety Analysis and the Alternative Hypothesis -- Forest Plots of Adverse Effects -- Graphics of Adverse Effects -- Repeated Measures Methods for Testing Adverse Effects -- Benefit Risk Ratios -- Equivalence, Non-inferiority and Superiority Testing of Adverse Effects -- Part II The Analysis of Dependent Adverse Effects -- Independent and Dependent Adverse Effects. Categorical Predictors Assessed as Dependent Adverse Effects. Adverse Effect of the Dependent Type in Crossover Trial -- Confoundings and Interactions Assessed as Dependent Adverse Effects -- Subgroup Characteristics Assessed as Dependent Adverse Effects -- Random Effects Assessed as Dependent Adverse Effects -- Outliers Assessed as Dependent Adverse Effects -- Index
    Abstract: The authors, as professors in statistics at various universities in Europe, are worrried about the poor quality of safety data analysis of clinical trials, despite its importance in drug development and pharmacovigilance. Clinical trials, not adequately addressing safety, are unethical. An effective approach for the purpose is to present summaries of prevalences. In order to estimate the probability, that the differences between treatment and control group did not occur merely by chance, a statistical test can be performed. This pretty crude method has recently be supplemented with better sensitive methodologies, based on machine learning clusters and networks, and multivariate analyses. Another important novelty with safety data analysis is the new insights into hypothesis testing, favoring the alternative hypotheses instead of the null hypotheses. Finally the issue of dependency is addressed. Adverse effects may be either dependent or independent of the main outcome. Dependent adverse effect are dependent not only on the treatment modalities, but also on the outcome of the trials. Random heterogeneities, outliers, confounders, interaction factors are common in clinical trials, and all of them can be considered kinds of adverse effects of the dependent type. Random regressions and analyses of variance, high dimensional clusterings, partial correlations, structural equations models, and other Bayesian methods are helpful for their analysis. The current edition was particularly written for medical and health professionals and students. It provides examples of modern analytic methods so far largely unused. All of the 16 chapters have two core characteristics, first they are for current usage, second they try and tell what readers need to know in order to understand the methods. Step by step analyses are given and self-assessment examples are supplied. Each chapter can be studied as a stand-alone
    Pages: XI, 217 p. 191 illus., 28 illus. in color. : online resource.
    ISBN: 9783030058043
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  • 20
    Keywords: Mathematics ; Mathematics Education ; History of Mathematical Sciences ; International and Comparative Education ; Springer eBooks
    Description / Table of Contents: Chapter 1. European Didactic Traditions in Mathematics: Introduction and Overview -- Chapter 2. The French Didactic Tradition in Mathematics -- Chapter 3. Didactics of mathematics in the Netherlands -- Chapter 4. The Italian Didactic Tradition.-Chapter 5. The German Speaking Didactic Tradition -- Chapter 6. Didactics of Mathematics as a Research Field in Scandinavia -- Chapter 7. Czech and Slovak Research in Didactics of Mathematics Tradition and a Glance at Present State
    Abstract: This open access book discusses several didactic traditions in mathematics education in countries across Europe, including France, the Netherlands, Italy, Germany, the Czech and Slovakian Republics, and the Scandinavian states. It shows that while they all share common features both in the practice of learning and teaching at school and in research and development, they each have special features due to specific historical and cultural developments. The book also presents interesting historical facts about these didactic traditions, the theories and examples developed in these countries
    Pages: V, 212 p. 28 illus., 10 illus. in color. : online resource.
    ISBN: 9783030055141
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  • 21
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Origin and Pathogenesis of B Cell Lymphomas -- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma -- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells -- PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations -- Expression Cloning of Antibodies from Single Human B Cells -- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR -- Stereotyped B Cell Immunoglobulins in B Cell Lymphomas -- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies -- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR -- Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input -- FISH and FICTION in Lymphoma Research -- NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma -- RNA Sequencing in B-Cell Lymphomas -- Studying Cancer Heterogeneity by Single Cell RNA-Sequencing -- Evaluation of Whole Genome Sequencing Data -- Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines -- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens -- Proximity Ligation Assay -- Phospoproteomic Analysis of Signaling Pathways in Lymphomas -- Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries
    Abstract: This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology
    Pages: XII, 439 p. 66 illus., 48 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991518
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  • 22
    Keywords: Medicine ; Cell Culture ; Nephrology ; Molecular Medicine ; Cell Culture ; Nephrology ; Springer eBooks
    Description / Table of Contents: Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development -- Mouse Ex Vivo Kidney Culture Methods -- Serum-Free Organ Culture of the Embryonic Mouse Ureter -- Experimental Tubulogenesis Induction Model in the Mouse -- Dissociation of Tissues for Single Cell Analysis -- Long Term Culture of Nephron Progenitor Cells Ex Vivo -- 3D Cell Culture Models of Epithelial Tissues -- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells -- Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells -- Protocol to Generate Ureteric Bud Structures from Human iPS Cells -- Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture -- Virus as Renal Functional Genetic Tools -- Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines -- Time-Lapse Technologies and 4D Imaging of Kidney Development -- Decellularizing and Recellularizing Adult Mouse Kidneys -- Optical Projection Tomography Imaging to Study Kidney Organogenesis -- Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model -- Analysis of the Teashirt Target Genes in Ureteric Bud Development -- Methods of Computational Analysis in Kidney Development
    Abstract: This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XI, 248 p. 57 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990214
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  • 23
    Keywords: Embryology ; Laboratory animals ; Animal genetics ; Embryology ; Animal Models ; Animal Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments -- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos -- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos -- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts -- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis -- Embryological and Genetic Manipulation of Chick Development -- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos -- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens -- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) -- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos -- Ex Utero Culture and Imaging of Mouse Embryos -- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections -- Reptile Embryology and Regeneration -- Manipulation of Developmental Function in Turtles with Notes on Alligators -- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes -- Visualizing the Balbiani Body in Zebrafish Oocytes -- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis -- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos -- Germ Cell Transplantation in Avian Species -- Production of Germ-Line Chimeras in Zebrafish -- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring -- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) -- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish -- Modeling Embryonic Cleavage Patterns -- Laboratory Guidelines for Animal Care
    Abstract: This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development. Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
    Pages: XI, 434 p. 82 illus., 70 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493990092
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  • 24
    Keywords: Biodiversity ; Computer science ; Geographical information systems ; Biodiversity ; Computer Applications ; Geographical Information Systems/Cartography ; Springer eBooks
    Description / Table of Contents: Global biodiversity data portals -- Information and analytical systems on biodiversity -- National data on biodiversity: the current state of digitization -- Theoretical fundaments and oraganization technologies of the information and telecommunications infrastructures -- Methods, models, software systems and Web services for the analysis of biodiversity data -- Application of remote methods in vegetation mapping and biodiversity research
    Abstract: This book offers a collection of papers presented at the First International Conference” Information Technologies in the Research of Biodiversity” that was held from 11-14 September 2018 in Irkutsk (Russia). Papers in this book cover areas of interaction of knowledge on biodiversity and information technologies. The main topics include: methods, models, software systems for the analysis of biodiversity data; global data portals; information and analytical systems on biodiversity; application of remote methods in vegetation mapping; theoretical fundaments and organization technologies of the information and telecommunications infrastructures
    Pages: XI, 202 p. 70 illus., 37 illus. in color. : online resource.
    ISBN: 9783030117207
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  • 25
    Keywords: Proteomics ; Biochemistry ; Proteomics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Pre- and Post-Analytical Factors in Biomarker Discovery -- Pre-Fractionation of Non-Circulating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers -- Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers -- Protein Biomarker Discovery Using Human Blood Plasma Microparticles -- A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry -- Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery -- Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies -- Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics -- Protein Biomarker Discovery in Non-Depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry -- Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry -- Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid -- Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions -- Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues -- Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications -- Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR -- Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis using CombiROC -- PanelomiX for the Combination of Biomarkers -- Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework
    Abstract: This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 292 p. 52 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991648
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  • 26
    Keywords: Medical parasitology ; Emerging infectious diseases ; Microbial genetics ; Microbial genomics ; Parasitology ; Infectious Diseases ; Microbial Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome -- Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development -- Transcriptome Studies in Trypanosoma cruzi using RNAseq -- An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene -- Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi -- Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes -- Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi -- Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae -- Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi -- Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase -- Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions -- Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues -- In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters -- Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models -- MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice -- Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR -- Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR -- Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase -- Analytics of Functional Autoantibodies in Patients with Chagas’ Disease -- Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease -- Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma -- Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease -- Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients -- Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients -- Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease -- CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease -- Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease -- Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts
    Abstract: This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information
    Pages: XVII, 398 p. 60 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991488
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  • 27
    Keywords: Cell receptors ; Lipids ; Receptors ; Lipidology ; Springer eBooks
    Description / Table of Contents: Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry
    Abstract: This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond
    Pages: XI, 220 p. 35 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991303
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  • 28
    Keywords: Cytology ; Biochemistry ; Cell Biology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: The Complex Interplay between Extracellular Matrix and Cells in Tissues -- Method for Studying ECM Expression: In Situ RT-PCR -- Visualizing the Supramolecular Assembly of Collagen -- Steady-state and Pulse-chase Analyses of Fibrillar Collagen -- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates -- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems -- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples -- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis -- Hyaluronan Pericellular Matrix: Particle Exclusion Assay -- Determination of Cell Surface Hyaluronan through Flow Cytometry -- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine -- Analyzing Hyaluronidases in Biological Fluids -- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating -- Methods for Monitoring Matrix-Induced Autophagy -- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography -- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography -- In Vitro Spheroid Sprouting Assay of Angiogenesis -- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis -- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization -- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding -- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
    Abstract: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology
    Pages: XII, 278 p. 49 illus., 27 illus. in color. : online resource.
    ISBN: 9781493991334
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  • 29
    Keywords: Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp. -- Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance -- Antimicrobial Susceptibility Testing Methods for Acinetobacter spp -- Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii -- Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper Supplemented Liquid Media and on Copper-Containing Surfaces -- A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy -- Transformation of Acinetobacter baumannii: Electroporation -- Methods for Natural Transformation in Acinetobacter baumannii -- Vesicle Mediated Gene Transfer in A. baumannii -- Targeted Gene Replacement in Acinetobacter baumannii -- RecET-Mediated Recombineering in Acinetobacter baumannii -- Methods for Tn-seq Analysis in Acinetobacter baumannii -- Tn7-Based Single Copy Insertion Vectors for Acinetobacter baumannii -- Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii -- A Simple Static Biofilm Assay for Acinetobacter baumannii -- BioFluxTM 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth -- In Vitro Motility Assays for Acinetobacter Species -- Desiccation Tolerance Assays for Acinetobacter baumannii -- Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure -- PBP Isolation and DD-Carboxypeptidase Assay -- Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii -- Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii -- Methods for Detecting N-acyl-homoserine Lactone Production in Acinetobacter baumannii -- Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii -- Skin and Soft Tissue Models for Acinetobacter baumannii Infection -- Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection -- Computational Prediction of sRNA in Acinetobacter baumannii -- Global Metabolic Analyses of Acinetobacter baumannii -- Reverse Vaccinology Approach to Potential Vaccine Candidates against Acinetobacter baumannii
    Abstract: This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study
    Pages: XIV, 338 p. 87 illus., 53 illus. in color. : online resource.
    ISBN: 9781493991181
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  • 30
    Keywords: Cytology ; Neurosciences ; Cell Biology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System -- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing -- Myelin: Methods for Purification and Proteome Analysis -- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells -- Isolation and Culture of Oligodendrocytes -- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation -- Oligodendrocyte-Neuron Myelinating Co-Culture -- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier -- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices -- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord -- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination -- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination -- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation -- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis -- Conditional Mutagenesis in Oligodendrocyte Lineage Cells -- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage -- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination -- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease -- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes -- Transmission Electron Microscopy of Oligodendrocytes and Myelin -- Toxin-Based Models to Investigate Demyelination and Remyelination -- Magnetic Resonance techniques for Imaging White Matter
    Abstract: This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function
    Pages: XII, 411 p. 65 illus., 55 illus. in color. : online resource.
    ISBN: 9781493990726
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  • 31
    Keywords: Toxicology ; Biochemistry ; Oncology ; Pharmacology/Toxicology ; Biological and Medical Physics, Biophysics ; Biochemistry, general ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- Chapter 1. Antimicrobial peptides of multicellular organisms: My perspective -- Part 2. Mechanisms of Antimicrobial Action -- Chapter 2. Membrane permeabilization mechanisms -- Chapter 3. Elementary processes and mechanisms of interactions of antimicrobial peptides with membranes --- single GUV studies ---- -- Chapter 4. The mechanisms of action of cationic antimicrobial peptides refined by novel concepts from biophysical investigations -- Chapter 5. Anionic lipid clustering model -- Chapter 6. Intracellular antimicrobial peptides targeting the protein synthesis machinery -- Part 3. Other activities of AMPs -- Chapter 7. Antimicrobial and cell-penetrating peptides: How to understand two distinct functions despite similar physicochemical properties -- Chapter 8. Synthetic anti-lipopolysaccharide peptides (SALP) as effective inhibitors of pathogen-associated molecular patterns (PAMPs) -- Chapter 9. Anti-cancer activities of natural and synthetic peptides -- Chapter 10. Antimicrobial host defence peptides: Immunomodulatory functions and translational prospects -- Part 4. Towards Clinical Applications -- Chapter 11. Activity and selectivity of antimicrobial peptides: A complex interplay of multiple equilibria -- Chapter 12. Design of antimicrobial peptides: Progress made with human cathelicidin LL-37 -- Chapter 13. Application of synthetic molecular evolution to the discovery of antimicrobial peptides -- Chapter 14. AMPs as anti-biofilm agents for human therapy and prophylaxis -- Chapter 15. Clinical application of AMPs
    Abstract: This book presents an overview of antimicrobial peptides (AMPs), their mechanisms of antimicrobial action, other activities, and various problems that must still be overcome regarding their clinical application. Divided into four major parts, the book begins with a general overview of AMPs (Part I), and subsequently discusses the various mechanisms of antimicrobial action and methods for researching them (Part 2). It then addresses a range of activities other than antimicrobial action, such as cell penetration, antisepsis, anticancer, and immunomodulatory activities (Part 3), and explores the prospects of clinical application from various standpoints such as the selective toxicity, design, and discovery of AMPs (Part 4). A huge number of AMPs have been discovered in plants, insects, and vertebrates including humans, and constitute host defense systems against invading pathogenic microorganisms. Consequently, many attempts have been made to utilize AMPs as antibiotics. AMPs could help to solve the urgent problem of drug-resistant bacteria, and are also promising with regard to sepsis and cancer therapy. Gathering a wealth of information, this book will be a bible for all those seeking to develop antibiotics, anti-sepsis, or anticancer agents based on AMPs
    Pages: VIII, 304 p. 73 illus., 55 illus. in color. : online resource.
    ISBN: 9789811335884
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  • 32
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    Cham : Springer
    Keywords: Biological models ; Human Genetics ; Endocrinology ; Cytology ; Systems Biology ; Human Genetics ; Endocrinology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: 1. History of the thymus: From a vestigial organ to the programming of immunological self-tolerance -- 2. Thymus Ontogeny and Development -- 3. The Ins and Outs of Thymic Epithelial Cell Differentiation and Function -- 4. T-Cell Development: From T-Lineage Specification to Intrathymic Maturation -- 5. Intrathymic cell migration: implications in thymocyte development and T-cell repertoire formation -- 6. Thymic crosstalk: an overview of the complex cellular interactions that control the establishment of T-cell tolerance -- 7. The Autoimmune Regulator (AIRE) gene, the master activator of self-antigen expression in the thymus -- 8. Aire Mutations and Autoimmune Diseases -- 9. The thymus as a mirror of the body's gene expression -- 10. Functional genomics of the infant human thymus: Aire and minipuberty -- 11. Thymus transcriptome of TGF-β superfamily -- 12. Development of thymic regulatory T lymphocytes -- 13. Adding insult to injury: improving the regenerative capacity of the aged thymus following clinically induced damage -- 14. Thymus rejuvenation after autologous hematopoietic stem cell transplantation in patients with autoimmune diseases
    Abstract: This volume focuses on challenging field in biomedicine that is the genetic control of central immune tolerance. It covers the thymus development, their cellular components and their respective function, the peculiar gene expression profiling (transcriptome) found in the medullary thymic epithelial cells (mTECs) that are implicated in the self-representation in the thymus, the Autoimmune regulator (Aire) gene, the mutations in this gene and manifestation of autoimmune diseases, and the role of cell-cell interactions within the thymus with implications in the negative selection (elimination) of nascent autoreactive T cells in preventing aggressive autoimmunity. The thymus gland is a lymphoid organ implicated in the maturation, differentiation and selection of T cells. This organ is gained more and more attention in different biomedical research labs worldwide due to its function that is associated with the control of immune homeostasis in the body, establishing the central immune tolerance and preventing the onset of autoimmune diseases
    Pages: XVII, 318 p. 30 illus., 23 illus. in color. : online resource.
    ISBN: 9783030120405
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  • 33
    Keywords: Human Genetics ; Bioinformatics ; Human Genetics ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: Chapter1. Strategies for converting RNA to amplifiable cDNA for single-cell RNA sequencing methods -- Chapter2. Integrated Fluidic Circuits for single cell omics and multi-omics applications -- Chapter3. Single-cell DNA-Seq and RNA-Seq in cancer using the C1 system -- Chapter4. Nx1-Seq (Well based Single-Cell Analysis System) -- Chapter5. Quantitation of mRNA transcripts and proteins using the BD Rhapsody™ Single-Cell Analysis System -- Chapter6. An Informative Approach to Single-Cell Sequencing Analysis -- Chapter7. Bionano Genome Mapping: High-Throughput, Ultra-Long Molecule Genome Analysis System for Precision Genome Assembly and Haploid-Resolved Structural Variation Discovery -- Chapter8. Informatics for PacBio long reads -- Chapter10. On-site MinION sequencing
    Abstract: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians
    Pages: VI, 150 p. 56 illus., 51 illus. in color. : online resource.
    ISBN: 9789811360374
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  • 34
    Keywords: Stem Cells ; Oncology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Pericytes in the retina -- Pancreatic pericytes in glucose homeostasis and diabetes -- Pericytes in the lung -- Pericytes in the skeletal muscle -- Pericytes in the gut -- Pericytes in the bone marrow -- Cochlear Capillary Pericytes -- Pericytes in the placenta: role in placental development and homeostasis -- Pericytes in the liver -- Pericytes in the Periodontal Ligament -- Pericytes in the heart -- Pericytes in the Umbilical Cord -- The pluripotent microvascular pericytes are the adult stem cells even in the testis -- Index
    Abstract: The present book is an attempt to describe the most recent developments in the area of pericyte biology which is one of the emergent hot topics in the field of molecular and cellular biology today. Here, we present a selected collection of thirteen detailed chapters on what we know so far about pericytes in distinct organs in physiological and pathological conditions. Further, it provides an update on the most novel functions attributed to these cells and will introduce a newer generation of researchers and scientists to the importance of these cells, ranging from their discovery in different organs through current state-of-the-science. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and vascular research. This volume explores pericytes' physiologic roles in different tissues, ranging from the pancreas, lungs and liver through skeletal muscle, gut, retina and more. Together with its companion volumes Pericyte Biology in Disease and Pericyte Biology – Novel Concepts, Pericyte Biology in Different Organs presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and researchers or clinicians involved with specific organs
    Pages: XI, 277 p. 60 illus., 51 illus. in color. : online resource.
    ISBN: 9783030110932
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  • 35
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    Cham : Springer
    Keywords: Human Genetics ; Developmental Biology ; Immunology ; Medical virology ; Human Genetics ; Developmental Biology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Part 1 Foundations for muscle gene therapy -- Chapter 1. An overview of muscle biology and physiology for muscle gene therapy -- Chapter 2. Molecular basis of muscle disease -- Chapter 3. Animal models for muscle disease and muscle gene therapy -- Chapter 4. Muscle stem cell biology and implication in gene therapy -- Chapter 5. Pluripotent stem cells for gene therapy of hereditary muscle disorders -- Chapter 6. MicroRNAs (miRs) in muscle gene therapy -- Chapter 7. Immune system regulation of muscle injury and disease -- Chapter 8. Design of muscle gene therapy expression cassette -- Chapter 9 Non-viral vector mediated muscle gene therapy -- Chapter 10. Viral vectors for muscle gene therapy -- Chapter 11. Development of next generation muscle gene therapy AAV vectors -- Chapter 12. Histological and biochemical evaluation of muscle gene therapy -- Chapter 13. Optical polarization tractography imaging of structural changes in the skeletal and cardiac muscles of the mdx4cv mice -- Chapter 14. Biomarkers for muscle disease gene therapy -- Chapter 15. Large-scale clinical manufacturing of AAV Vectors for systemic muscle gene therapy -- Chapter 16. Genome-editing for muscle gene therapy -- Part 2 Preclinical muscle gene therapy -- Chapter 17. Considerations on preclinical muscle gene therapy studies -- Chapter 18. Gene replacement therapy for Duchenne muscular dystrophy -- Chapter 19. Recent advances in AON-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 20. AAV-mediated exon-skipping therapy for Duchenne muscular dystrophy -- Chapter 21. Alternate translational initiation of dystrophin: a novel therapeutic approach -- Chapter 22. Genome editing for Duchenne muscular dystrophy -- Chapter 23. Sarcolipin knockdown therapy for Duchenne muscular dystrophy -- Chapter 24. Gene therapy for central nervous system in Duchenne muscular dystrophy -- Chapter 25. Therapeutic approach for dysferlinopathy in animal models -- Chapter 26. Muscle cell membrane repair and therapeutic implications -- Chapter 27. Treatment of muscular dystrophy-dystroglycanopathy: unlocking the potential of genetic engineering -- Chapter 28. RNAi therapy for dominant muscular dystrophies and other myopathies -- Chapter 29. Gene therapy for facioscapulohumeral muscular dystrophy (FSHD) -- Chapter 30. Gene therapy and gene editing for myotonic dystrophy -- Chapter 31. Gene therapy for oculopharyngeal muscular dystrophy -- Chapter 32. Gene therapy for X-linked myotubular myopathy -- Chapter 33. Pre-clinical gene therapy studies for metabolic myopathy -- Chapter 34 Elimination of mutant mitochondrial DNA in mitochondrial myopathies using gene editing enzymes -- Chapter 35. Gene therapy for Charcot-Marie-Tooth inherited neuropathy -- Chapter 36. Muscle - a potent target in vaccination -- Part 3 Clinical muscle gene therapy -- Chapter 37. Patient and family perspective on muscle gene therapy -- Chapter 38. Design of clinical trials for gene therapy in muscular dystrophy -- Chapter 39. Path to clinical trials: trial design, development of the clinical product and safety concerns in the implementation of clinical trials -- Chapter 40. Muscle MRI as an endpoint in clinical trials -- Chapter 41. Gene therapy clinical trials for Duchenne and limb girdle muscular dystrophies lessons learned -- Chapter 42. Duchenne muscular dystrophy exon-skipping trials -- Chapter 43. What we have learned from 10 years of DMD exon skipping trials -- Chapter 44. Clinical gene therapy trials for Pompe disease -- Chapter 45. Muscle-directed gene therapy for alpha-1 antitrypsin deficiency
    Abstract: About 7 million people worldwide are suffering from various inherited neuromuscular diseases. Gene therapy brings the hope of treating these diseases at their genetic roots. Muscle Gene Therapy is the only book dedicated to this topic. The first edition was published in 2010 when the field was just about to enter its prime time. The progress made since then has been unprecedented. The number of diseases that have been targeted by gene therapy has increased tremendously. The gene therapy toolbox is expanded greatly with many creative novel strategies (such as genome editing and therapy with disease-modifying genes). Most importantly, clinical benefits have begun to emerge in human patients. To reflect rapid advances in the field, we have compiled the second edition of Muscle Gene Therapy with contributions from experts that have conducted gene therapy studies either in animal models and/or in human patients. The new edition offers a much needed, up-to-date overview and perspective on the foundation and current status of neuromuscular disease gene therapy. It provides a framework to the development and regulatory approval of muscle gene therapy drugs in the upcoming years. This book is a must-have for anyone who is interested in neuromuscular disease gene therapy including those in the research arena (established investigators and trainees in the fields of clinical practice, veterinary medicine and basic biomedical sciences), funding and regulatory agencies, and patient community
    Pages: XVI, 811 p. 78 illus., 58 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9783030030957
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  • 36
    Keywords: Biomedical Engineering ; Computer engineering ; Biomedical Engineering/Biotechnology ; Biomedical Engineering and Bioengineering ; Signal, Image and Speech Processing ; Biological and Medical Physics, Biophysics ; Electrical Engineering ; Springer eBooks
    Description / Table of Contents: Part I Signal Processing for Physiology -- Introduction to Measurement and Analysis in Physiology -- Basic Concepts in Systems Theory and Signal Processing -- Signal Filtering and System Control for Physiology -- Digitization and Discrete Systems - Sampling and Quantization -- Discrete Signal Processing for Physiological Signals -- Graphics and Haptics for Simulation Models -- Model Based Analysis of Physiological Systems -- Part II Models of Physiological Systems -- Nerve Communication I - Wave Propagation and Action Potentials -- Neural Communication II - Firing Rate and Information Encoding -- External Stimulation of Nerves in Tissue - From Theory to Practice -- Skeletal Muscle Contraction - Force and Movement -- The Electromyogram - Modeling and Analysis -- Neuromuscular Control - Spinal Reflex and Movement -- Cardiovascular Blood Flow Modeling -- Immune Response to Infection
    Abstract: Physiology is a set of processes that maintain homeostasis, and physiological measurement is a means of observing these processes. Systems theory and signal processing offer formal tools for the study of processes and measured quantities. This book shows that systems modeling can be used to develop simulations of physiological systems, which use formal relations between the underlying processes and the observed measurements. The inverse of such relations suggest signal processing tools that can be applied to interpret experimental data. Both signal processing and systems modeling are invaluable in the study of human physiology. Discussing signal processing techniques ranging from filtering and spectrum analysis to wavelet analysis, the book also includesGraphs and analogies to supplement the mathematics and make the book more accessible to physiologists and also more interesting to engineers. Physiological systems modeling helps in both gaining insights and generating methods of analysis. This book shows how numerical computation with graphical display, haptics and multimedia can be used to simulate physiological systems. In this third edition the simulations are more closely related to clinical examination and experimental physiology than in previous editions. Detailed models of nerve and muscle at the cellular and systemic levels, and simplified models of cardiovascular blood flow provide examples for the mathematical methods and computer simulations. Several of the models are sufficiently sophisticated to be of value in understanding real world issues like neuromuscular disease. The book features expanded problem sets and a link to extra downloadable material, and simulation programs that are solutions to the theory developed in the text are also available
    Pages: XV, 468 p. 362 illus., 123 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9789811335310
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  • 37
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    New York, NY : Springer
    Keywords: Orthopedics ; Sports Medicine ; Orthopedics ; Sports Medicine ; Springer eBooks
    Description / Table of Contents: Nonsurgical Hammertoe Treatment Options -- PIPJ Arthroplasty without Fixation -- PIPJ Interpositional Arthroplasty -- PIPJ Arthroplasty with Temporary Kirchner Wire Fixation -- PIPJ Arthrodesis, Peg and Hole -- PIPJ Arthrodesis via Buried Kirchner Wire -- PIPJ Arthrodesis with a 1 Component Metallic Implant -- PIPJ Arthrodesis with a 2 Component Metallic Implant -- PIPJ Arthrodesis with a Thermal Activated Metallic Implant -- PIPJ Arthrodesis with an Allograft Implant -- PIPJ Arthrodesis with a Bioabsorbable Implant -- Flexor Tendon Transfer -- Tenotomies -- MTP Joint: Soft Tissue Balancing (skin plasties and lengthenings, MTPJ release, extensor tendon lengthening, capsulotomy, capsulorraphy) -- MTP Joint: Plantar Plate Reconstruction via a Dorsal Approach -- MTP Joint: Plantar Plate Repair via a Plantar Approach -- Lesser Metatarsal Osteotomies: uniplanar, biplanar wedge osteotomies -- MTP Joint: Transverse Plane Correction via Collateral Ligament Repair -- MTP Joint Transverse Plane Correction via Metatarsal Osteotomy -- Crossover Toe Correction -- Congenital Overlapping Fifth Toe -- Flail Hammertoe Correction
    Abstract: Comprised exclusively of clinical cases demonstrating the various management strategies for hammertoes, this concise, practical casebook will provide foot and ankle surgeons with the best real-world strategies to properly treat these frequent deformities of the lower extremity and their complications. Beginning with a review of the relevant anatomy and biomechanics of the toe, each subsequent chapter includes case material with a unique clinical presentation, followed by a description of the diagnosis, assessment and management techniques used to treat it, as well as the case outcome, literature review and clinical pearls and pitfalls. Chapters included illustrate different surgical fixation techniques, including PIPJ arthroplasty and arthrodesis, tendon transfers and tenotomies, and osteotomies, as well as management strategies for complications. Pragmatic and reader-friendly, Hammertoes: A Case-Based Approach is an excellent resource for foot and ankle surgeons treating this common but frustrating condition
    Pages: XIII, 419 p. 511 illus., 427 illus. in color. : online resource.
    ISBN: 9783319165523
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  • 38
    Keywords: Biochemistry ; Bioinformatics ; Protein Structure ; Bioinformatics ; Springer eBooks
    Description / Table of Contents: 55 Years of the Rossmann Fold -- Advances in Protein Super-Secondary Structure Prediction and Application to Protein Structure Prediction -- Automated Family-Wide Annotation of Secondary Structure Elements -- Computational Prediction of Secondary and Supersecondary Structures from Protein Sequences -- StackSSSPred: A Stacking Based Prediction of Supersecondary Structure from Sequence -- Information-Theoretic Inference of an Optimal Dictionary of Protein Supersecondary Structures -- Secondary and Supersecondary Structure of Proteins as Coupling between Local and Local and Backbone-Electrostatic Interactions: A View through Cluster-Cumulant Scope -- Learning Organizations of Protein Energy Landscapes: An Application on Decoy Selection in Template-Free Protein Structure Prediction -- Exploring Protein Supersecondary Structure through Changes in Protein Folding, Stability, and Flexibility -- Protodomains: Symmetry Related Supersecondary Structures in Proteins and Self-Complementarity -- bab Super Secondary Motifs: Sequence, Structural Overview and Pursuit of Potential Autonomously Folding bab Sequences from (b/a)8/TIM Barrels -- Formation of Cross-Beta Supersecondary Structure by Soft-Amyloid Cores: Strategies for their Prediction and Characterization -- Stable Sub-Structures in Proteins and How to Find Them using Single-Molecule Force Spectroscopy -- Supersecondary Structures and Fragment Libraries -- Molecular Dynamics Simulations of Conformational Conversions in Transformer Proteins -- Sequence Pattern for Supersecondary Structure of Sandwich-Like Proteins -- Homology Searches using Supersecondary Structure Code -- Beyond Supersecondary Structure: Physics-Based Sequence Alignment -- Secondary and Supersecondary Structure of Proteins in Light of the Structure of Hydrophobic Cores -- Hands On: Using Tryptophan Fluorescence Spectroscopy to Study Protein Structure -- Structural Characterization of Membrane Protein Dimers
    Abstract: This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation
    Pages: XVIII, 438 p. 119 illus., 103 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991617
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  • 39
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Setting Up a Single-Cell Genomic Laboratory -- Tissue Handling and Dissociation for Single-Cell RNA-seq -- Full-Length Single-Cell RNA-Sequencing with Smart-seq2 -- CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification -- Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq) -- Single-Cell RNA Sequencing with Drop-Seq -- Chromium 10X Single Cell 3’ mRNA Sequencing of Tumor Infiltrating Lymphocytes -- Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-Sequencing -- Single-Cell Tagged Reverse Transcription (STRT-seq) -- Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQ -- High Throughput Single Cell Real-Time Quantitative PCR Analysis -- Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ System -- Targeted TCR Amplification from Single-Cell cDNA Libraries -- Sequencing the Genomes of Single Cells -- Studying DNA Methylation in Single Cell Format with scBS-seq -- Single Cell 5fC Sequencing -- ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA Interactions -- Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry -- Classification of the Immune Composition in the Tumor Infiltrate -- Combined Genome and Transcriptome (G&T) Sequencing of Single Cells -- Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single Cell -- Simultaneous Targeted Detection of Proteins and RNAs in Single Cells -- CRISPR-Screening in Single Cells -- Single Cell Live Imaging -- Differential Expression Analysis in Single Cell Transcriptomics -- A Bioinformatic Toolkit for Single-Cell mRNA Analysis
    Abstract: This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab
    Pages: XVI, 459 p. 87 illus., 78 illus. in color. : online resource.
    ISBN: 9781493992409
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  • 40
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains -- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta -- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA -- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions -- Quantification of Extracellular ATP in Plant Suspension Cell Cultures -- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions -- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato -- Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIM -- Analysis of DNA Methylation Profile in Plants by Chop-PCR -- Change in Nucleosome Dynamics during Stress Responses in Plants -- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry -- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments -- Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae -- Quantification of Cauline Leaf Abscission in Response to Plant Pathogens -- Methods for Replicating Leaf Vibrations Induced by Insect Herbivores -- Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes -- The Apple Fruitlet Model System for Fire Blight Disease -- Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins -- Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems -- Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants -- Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth -- A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance
    Abstract: Back Cover Copy The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field
    Pages: XIII, 260 p. 59 illus., 42 illus. in color. : online resource.
    ISBN: 9781493994588
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  • 41
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    Singapore : Springer
    Call number: QH324:49
    Keywords: Bioinformatics ; Medicine ; Statistics ; Biomedicine general ; Statistics for Life Sciences, Medicine, Health Sciences
    Description / Table of Contents: Part 1. BIOINFORMATICS FOR LIFE AND PERSONAL GENOME INTERPRETATION -- Chapter 1. Bioinformatics For Life -- Chapter 2. Next Generation Sequencing and Personal Genome Data Analysis -- Chapter 3. Personal Genome Data Analysis -- Chapter 4. Personal Genome Interpretation and Disease Risk Prediction -- Part 2. ADVANCED MICROARRAY DATA ANALYSIS -- Chapter 5. Advanced Microarray Data Analysis -- Chapter 6. Gene Expression Data Analysis -- Chapter 7. Gene Ontology and Biological Pathway-based Analysis -- Chapter 8. Gene-set Approaches and Prognostic Subgroup Prediction -- Chapter 9. MicroRNA Data Analysis -- Part 3. NETWORK BIOLOGY, SEQUENCE, PATHWAY AND ONTOLOGY INFORMATICS -- Chapter 10. Network Biology, Sequence, Pathway and Ontology Informatics -- Chapter 11. Motif and Regulatory Sequence Analysis -- Chapter 12. Molecular Pathways and Gene Ontology -- Chapter 13. Biological Network Analysis -- Part 4. SNPS, GWAS AND CNVS, INFORMATICS FOR GENOME VARIANTS -- Chapter 14. SNPs, GWAS, CNVs: Informatics for Human Genome Variations -- Chapter 15. SNP Data Analysis -- Chapter 16. GWAS Data Analysis -- Chapter 17. CNV Data Analysis -- Part 5. METAGENOME AND EPIGENOME, BASIC DATA ANALYSIS -- Chapter 18. Metagenome and Epigenome Data Analysis -- Chapter 19. Metagenome Data Analysis -- Chapter 20. Epigenome Databases and Tools -- Chapter 21. Epigenome Data Analysis -- Appendix A. BASIC PRACTICE USING R FOR DATA ANALYSIS -- Appendix B. APPLICATION PROGRAM FOR GENOME DATA ANALYSIS INSTALL GUIDE
    Abstract: This textbook describes recent advances in genomics and bioinformatics and provides numerous examples of genome data analysis that illustrate its relevance to real world problems and will improve the reader’s bioinformatics skills. Basic data preprocessing with normalization and filtering, primary pattern analysis, and machine learning algorithms using R and Python are demonstrated for gene-expression microarrays, genotyping microarrays, next-generation sequencing data, epigenomic data, and biological network and semantic analyses. In addition, detailed attention is devoted to integrative genomic data analysis, including multivariate data projection, gene-metabolic pathway mapping, automated biomolecular annotation, text mining of factual and literature databases, and integrated management of biomolecular databases. This textbook is primarily intended for life scientists, medical scientists, statisticians, data processing researchers, engineers, and other beginners in bioinformatics who are experiencing difficulty in approaching the field. However, it will also serve as a simple guideline for experts unfamiliar with the new, developing subfield of genomic analysis within bioinformatics
    Pages: xvi, 367 p. : ill.
    ISBN: 9789811319419
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  • 42
    Keywords: Medicine ; Cardiology ; Oncology ; Cytology ; Molecular Medicine ; Cardiology ; Cancer Research ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Advancements in Managing Intracerebral Hemorrhage: Transition from Nihilism to Optimism -- Mandibular Advancement Devices in Patients with Symptoms of Obstructive Sleep Apnea: A Review -- Online Health Technologies and Mobile Devices: Attitudes, Needs and Future -- Radiological Response and Neutrophil-to-Lymphocyte Ratio as Predictive Factors for Progression-Free and Overall Survival in Metastatic Renal Cell Carcinoma Patients Treated with Sunitinib -- Plasminogen Activator Inhibitor Type 1 in Blood at Onset of Chemotherapy Unfavorably Affects Survival in Primary Ovarian Cancer -- Sialidase Attenuates Epidermal Growth Factor Response and Abolishes Antiproliferative Effects of Erlotinib in A549Alveolar Epithelial Cells -- Systemic Sclerosis and Serum Content of Transforming Growth Factor -- Protein-Bound Solute Clearance during Hemodialysis -- Mold Sensitization in Asthmatic and Non-Asthmatic Subjects Diagnosed with Extract-Based versus Component-Based Allergens -- Fructose Consumption and Lipid Metabolism in Obese Children and Adolescents -- Vaccination against Measles, Mumps, and Rubella in the Light of Current Epidemic Threats: Unjustified Postponement -- Variability of Dry-Eye Disease Following Removal of Lacrimal Glands in Rats
    Abstract: This book shares the latest research and practice-oriented findings in medical sciences with a wide audience. It addresses a range of contemporary issues, often unresolved or contentious, across various medical fields, including advances in the management of hemorrhagic brain stroke. It also discusses metastatic renal cell carcinoma – a global scourge with an extremely poor long-term survival prognosis, the course and sequelae of renal cell carcinoma, as well as advances in targeted molecular therapy with sunitinib, a receptor tyrosine kinase inhibitor. Further, it examines the molecular targeting of proliferative signaling of the epidermal growth factor receptor in the first-line treatment of patients with metastatic non-small-cell lung cancer. Other articles cover clearance of toxins in hemodialyzed patients; the search for diagnostic and therapeutic markers in the connective tissue disease scleroderma; obesity linked to inappropriate dietary habit; clinical problems related to the diagnosis of sensitization to fungi and its role in asthma; and reasons for the perilous trend of avoiding basic vaccinations in children. Lastly, the book explores the rapid developments in e-health technologies that increase access to health services, particularly for the elderly. The book is intended for clinical specialists, researchers, and all allied health professionals from various fields
    Pages: VI, 115 p. 17 illus., 10 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030190590
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  • 43
    Keywords: Stem Cells ; Oncology ; Cell Cycle ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Cancer Research ; Cell Cycle Analysis ; Springer eBooks
    Description / Table of Contents: Stem cells heterogeneity -- Heterogeneity in epiblast stem cells -- Progenitor cell heterogeneity in the adult carotid body germinal niche -- Planarian stem cell heterogeneity -- The cellular origin of Barrett’s esophagus and its stem cells -- Pluripotent stem cell heterogeneity -- Sarcoma stem cell heterogeneity -- Heterogeinity in adipose derived stem cells -- Unveiling stem cell heterogeneity towards the development of salivary gland regenerative strategies -- Heterogeneity of human mesenchymal stromal/stem cells -- Mitochondrial heterogeneity in stem cells -- The heterogeneity of renal stem cells, and their interaction with bio- and nano-materials -- Index
    Abstract: This book presents a comprehensive discussion on the novel concepts in stem cell heterogeneity, from pluripotent stem cells to human mesenchymal stem cells, adult and cancer stem cells of the thyroid, sarcoma, and more. Thus, Stem Cell Heterogeneity - Novel Concepts starts from a timely update on the current information on stem cells heterogeneity in various tissues and discusses new concepts and future directions. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity in Different Organs, and Stem Cells Heterogeneity in Cancer, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians
    Pages: XII, 225 p. 45 illus., 38 illus. in color. : online resource.
    ISBN: 9783030110963
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  • 44
    Keywords: Biomedical Engineering ; Medical records / Data processing ; Biomedical Engineering/Biotechnology ; Health Informatics ; Health Informatics ; Biomedical Engineering and Bioengineering ; Springer eBooks
    Description / Table of Contents: 1. A literature Review of Social Media-based Data Mining for Health Outcomes Research -- 2. Social Media-Based Health Interventions: Where Are We Now? -- 3. Quantifying and Visualizing the Research Status of Social Media & Health Research Field -- 4. Social Media in Health Communication -- 5. Consumers' Selection of Sources in Searching for Health Information -- 6. Understanding and Bridging the Language and Terminology Gap between Health Professionals and Consumers using Social Media -- 7. Dissemination of Information on Stigmatized Health Issues on Social Media -- 8. Learning Wellness Profiles of Users on Social Networks: The Case of Diabetes -- 9. Social Media and Psychological Disorder -- 10. Content Analysis of the 2015 #SmearForSmear Campaign Using Deep Learning -- 11. How to Improve Public Health via Mining Social Media Platforms: A Case Study of Human Papillomaviruses (HPV) -- 12. Learning Hormonal Therapy Medication Adherence from an Online Breast Cancer Forum -- 13. Ethics in Health Research using Social Media
    Abstract: This book presents state-of-the-art research methods, results, and applications in social media and health research. It aims to help readers better understand the different aspects of using social web platforms in health research. Throughout the chapters, the benefits, limitations, and best practices of using a variety of social web platforms in health research are discussed with concrete use cases. This is an ideal book for biomedical researchers, clinicians, and health consumers (including patients) who are interested in learning how social web platforms impact health and healthcare research
    Pages: XIII, 272 p. 31 illus., 19 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030147143
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  • 45
    Keywords: Microbiology ; Microbial ecology ; Marine Sciences ; Microbiology ; Microbial ecology ; Marine & Freshwater Sciences ; Springer eBooks
    Abstract: This book discusses how aquatic microbial communities develop interactive metabolic coordination both within and between species to optimize their energetics. It explains that microbial community structuration often includes functional stratification among a multitude of organisms that variously exist either suspended in the water, lodged in sediments, or bound to one another as biofilms on solid surfaces. The authors describe techniques that can be used for preparing and distributing microbiologically safe drinking water, which presents the challenge of successfully removing the pathogenic members of the aquatic microbial community and then safely delivering that water to consumers. Drinking water distribution systems have their own microbial ecology, which we must both understand and control in order to maintain the safety of the water supply. Since studying aquatic microorganisms often entails identifying them, the book also discusses techniques for successfully isolating and cultivating bacteria. As such, it appeals to microbiologists, microbial ecologists and water quality scientists
    Pages: XIII, 351 p. 100 illus., 38 illus. in color. : online resource.
    ISBN: 9783030167752
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  • 46
    Keywords: Pharmaceutical technology ; Pharmacy ; Pharmaceutical Sciences/Technology ; Pharmacy ; Springer eBooks
    Description / Table of Contents: Evolving Challenges in Developing Naturally-Derived Complex Mixtures into Drugs: U.S. Experience and Perspective -- Regulatory Landscapes for Approval of Naturally-derived Complex Mixture Drugs -- Complex Natural Product Heparin: Biosynthesis, Biology and Application via Synthetic Heparins -- Traditional Chinese Medicine Herbal Drugs: From Heritage to Future Developments -- Analytics on Farm-Dust Extract for Development of Novel Strategies to Prevent Asthma and Allergic Disease -- Analytical Tools for Physicochemical Characterization and Fingerprinting -- NMR in the Characterization of Complex Mixture Drugs -- Mass Spectrometry in the Characterization of Complex Drugs -- Scientific Considerations in the Approval of Complex Generi -- Raw Materials Production and Manufacturing Process Control Strategies -- Heparin Contamination and Issues Related to Raw Materials and Controls -- Pharmacology and Toxicology Concerns of Impurities -- Immunogenicity risks for Naturally Derived Complex Drugs -- Development of Plant-Derived Mixtures as Botanical Drugs: Clinical Considerations -- A Totality-of-Evidence Approach to Ensuring Therapeutic Consistency of Naturally Derived Complex Mixtures -- Economic Considerations for Complex Mixture Drugs -- Drug Discovery from Complex Mixtures: Serendipity, Screening and Characterization -- Integrated Approach for Characterization of Highly Heterogeneous Drugs
    Abstract: This volume in the AAPS Advances series covers various quality, safety and clinical aspects of drug development that are relevant to new and/or generic drugs containing a complex mixture of molecules. Specific topics discussed include: raw materials sourcing; manufacturing controls; characterization; identification of critical product quality components and attributes; identification of impurities, particularly as they bear on toxicity and immunogenicity; clinical trial study design considerations, and the regulatory science applications to development of such complex mixtures. Complex mixtures are challenging to characterize and analyze using standard methods. Further challenges extend throughout the product development cycle from raw material control to clinical study design. The regulatory landscape is rapidly changing as new types of complex mixtures are introduced into clinical trials and to the market (e.g., traditional Chinese medicines and medical marijuana products), while older products are facing generic competition for the first time (e.g., enoxaparin). The future outlook for complex generic drug products, as opposed to the more commonly developed targeted single agent drug products is not clear. The risks pertaining to lack of a full understanding of raw material control, process and controls in manufacture, as well as characterization of a complex mixture were seen vividly during the heparin crisis of 2008. As such powerful lessons have been learned about the regulatory science specific to complex products. The Science and Regulations of Naturally Derived Complex Drugs addresses the interests among industry, academics, and government on the issues surrounding the future development of mixtures for medicinal use
    Pages: VI, 331 p. 61 illus., 41 illus. in color. : online resource.
    ISBN: 9783030117511
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  • 47
    Keywords: Toxicology ; Pharmacology/Toxicology ; Drug Safety and Pharmacovigilance ; Springer eBooks
    Description / Table of Contents: Complexity of Biologics CMC Regulation -- Biopharmaceutics are Not Chemical Drugs -- An Effective CMC Strategy is Possible -- Challenge of Adventitious Agent Control -- Biopharmaceutical Source Materials -- Manufacturing of Biopharmaceutical APIs -- Manufacturing of the Drug Product -- Complex Process-Related Impurity Profiles -- Product Characterization is a Journey -- Priceless Potency (Therapeutic Activity) -- Quality Attributes of a Biopharmaceutical -- Designing the Stability Program -- The Art of Setting Specifications -- Demonstrating Product Comparability After Process Changes -- Invaluable CMC-Focused Meetings with Regulatory Authorities
    Abstract: This book since first published in 2004 has been a major resource providing insights and practical guidance for the CMC teams to develop an acceptable cost-effective, risk-based CMC regulatory compliance strategy for biopharmaceuticals (recombinant proteins, monoclonal antibodies, genetically engineered viruses and genetically engineered human cells) from early clinical stage development through market approval. The third edition of this book provides added coverage for the biosimilars, antibody drug conjugates (ADCs), bispecific antibodies, genetically engineered viruses, and genetically engineered cells. This third edition of the book also addresses the heightened pressure on CMC regulatory compliance timelines due to the introduction of expedited clinical pathways moving the clinical development closer to a seamless phase process (e.g., FDA Breakthrough Therapy designation, CBER Regenerative Medicine Advanced Therapy (RMAT) designation, EMA Priority Medicines (PRIME) designation). The Challenge of CMC Regulatory Compliance for Biopharmaceuticals is essential, practical information for all pharmaceutical development scientists, Manufacturing and Quality Unit staff, Regulatory Affairs personnel, and senior management involved in the manufacture of biopharmaceuticals
    Pages: XXVIII, 426 p. 59 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9783030137540
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  • 48
    Keywords: Human Physiology ; Cardiovascular System ; Immunology ; Developmental Biology ; Biomedical Engineering ; Human Physiology ; Cardiovascular Biology ; Immunology ; Developmental Biology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: 1. Morphological and Functional Characteristics of Blood and Lymphatic Vessels -- 2. The Heart: The Engine in the Center of the Vascular System -- 3. Regulation of Tissue Perfusion and Exchange of Solutes, Macromolecules, and Water between Blood Vessels and the Interstitial Space -- 4. Endothelial Cells: Function and Dysfunction -- 5. Vascular Smooth muscle cells: Regulation of Vasoconstriction and Vasodilation -- 6. Embryonic Development of the Cardiovascular System -- 7. Cellular and Molecular Mechanisms of Vasculogenesis, Angiogenesis, and Lymphangiogenesis -- 8. Mechanisms of Hemostasis: Contribution of Platelets, Coagulation Factors, and the Vessel Wall -- 9. Biologically active lipids in Vascular Biology -- 10. Atherosclerosis -- 11. Venous Thromboembolism -- 12. Genetics of Vascular Diseases -- 13. Animal Models in Cardio-Vascular Biology -- 14. Endothelial Cell Isolation and Manipulation -- 15. In vitro Assays Used to Analyse Vascular Cell Functions -- 16. The Porcine Coronary Artery Ring Myograph System -- 17. Proteomics in Vascular Biology
    Abstract: This well-structured textbook offers essential knowledge on the vascular system. The reader will learn the properties, basic cellular mechanisms and development of the different parts of the vascular system (including the heart), gain knowledge on vascular and related diseases, and will be made familiar with common and most current methods and techniques applied to analyze the vascular system in patients, in animal models, and ex vivo. This book is based on a PhD Course for students from various bioscientific backgrounds given at the Medical University of Vienna, and it will be a valuable resource for Master´s Students in vascular biology and biomedicine in general and a helpful tool for young researchers world-wide wishing to gain or refresh their knowledge in this field
    Pages: XX, 392 p. 73 illus., 70 illus. in color. : online resource.
    ISBN: 9783030122706
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  • 49
    Keywords: Biochemistry ; Toxicology ; Neurobiology ; Protein Science ; Pharmacology/Toxicology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics -- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs -- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies -- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties -- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions -- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands -- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs -- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology -- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches -- Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays -- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions -- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters -- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays -- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay -- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry -- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy -- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies -- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy -- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy -- Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug -- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice -- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents -- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs
    Abstract: This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com
    Pages: XV, 406 p. 87 illus., 71 illus. in color. : online resource.
    ISBN: 9781493991211
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  • 50
    Keywords: Neurochemistry ; Biomedical Engineering ; Neurochemistry ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: 1. Past, present and future of neuronal models in vitro -- 2. In vitro models of neural disorders -- 3. Neuronal cultures and nanomaterials -- 4. Advances in Human Stem Cell Derived Neuronal Cell Culturing and Analysis -- 5. Large scale, high-resolution microelectrode arrays for interrogation of neurons and networks -- 6. Multi-site intracellular recordings -- 7. From MEAs to MOAs: the next generation of bioelectronic interfaces for neuronal cultures -- 8. Scaling spike detection and sorting for next generation electrophysiology -- 9. Burst detection methods -- 10. Reconstruction of functional connectivity from multi-electrode recordings and calcium imaging -- 11. Open-source tools for processing and analysis of in vitro extracellular neuronal signals -- 12. Screening chemicals for potential neurotoxicity and developmental neurotoxicity using neural networks grown on MEAs -- 13. Long-term activity dynamics of single neurons and networks -- 14. Electrical and optical closed-loop systems
    Abstract: This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies
    Pages: XV, 387 p. 102 illus., 90 illus. in color. : online resource.
    ISBN: 9783030111359
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  • 51
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    Singapore : Springer
    Keywords: Medicine ; Biochemistry ; Molecular Medicine ; Protein Science ; Springer eBooks
    Description / Table of Contents: Part 1: Overview of Periostin -- Chapter 1: Naming, History, Future -- Part 2: Basic properties of Periostin -- Chapter 2: The structure of the periostin gene, its transcriptional control and alternative splicing, and protein expression -- Part 3: Function as the scaffold -- Chapter 3: Periostin functions as a scaffold for assembly of extracellular proteins -- Part 4: Health and Disease in Organs -- Chapter 4: Periostin reexpression in heart disease contributes to cardiac interstitial remodeling by supporting the cardiac myofibroblast phenotype -- Chapter 5: Periostin in bone biology -- Chapter 6: Periostin in bone regeneration -- Chapter 7: Functions of periostin in dental tissues and its role in periodontal tissue regeneration -- Chapter 8: Periostin and human teeth -- Chapter 9: Ability of periostin as a new biomarker of idiopathic pulmonary fibrosis -- Chapter 10: Involvement of periostin in skin function and the pathogenesis of skin diseases -- Chapter 11: Periostin in the kidney -- Chapter 12: Periostin in eye diseases -- Chapter 13: The maltiaspect functions of periostin in tumor progression -- Part 5: Other types of fibrosis and tissue repair -- Chapter 14: Liver, Stroke, Rhinosinusitis -- Part 6: Inflammation -- Chapter 15: Roles of periostin in asthma -- Part 7: Periostin in Development -- Chapter 16: Periostin and integrin signaling in stem cell regulation -- Chapter 17: Role of periostin in cardiac valve development -- Part 8: Biomarker -- Chapter 18: Practical application of periostin as a biomarker for pathological conditions -- Part 9: Clinical applications -- Chapter 19: Clinical trials and new applications
    Abstract: This book provides a comprehensive and up-to-date review of current understanding of periostin and its importance for human health and disease. Periostin is a secretory matricellular protein that has been revealed to play key roles in fibrillogenesis and cell migration, including metastasis of cancer cells. The production of periostin is upregulated during fibrotic responses and the mechanisms by which it promotes fibrosis have become a focus of interest owing to the potential clinical benefit to be derived from periostin blockade. In this book, readers will find coverage of all aspects, from the basic properties of periostin and its function as a scaffold for assembly of extracellular proteins through to its roles in bone and tissue regeneration, tumorigenesis, myocardial infarction, inflammatory and immune system disorders, and other diseases. Readers will also find the latest information on functions of periostin related to stemness and the application of periostin as a biomarker. It is hoped that the detailed knowledge of periostin and its pathobiological significance provided in this book will aid in the search for effective treatments for currently incurable diseases
    Pages: VIII, 213 p. 48 illus., 40 illus. in color. : online resource.
    ISBN: 9789811366574
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  • 52
    Keywords: Pharmaceutical technology ; Analytical biochemistry ; Biochemistry ; Pharmaceutical Sciences/Technology ; Analytical Chemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recognition Mechanisms of Chiral Selectors: An Overview -- Enantioseparation by Thin Layer Chromatography -- Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase -- Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS -- Chiral Mobile Phase Additives in HPLC Enantioseparations -- Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High Performance Liquid Chromatography: An Overview -- HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions -- Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC -- HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases -- Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector -- Cyclofructans as Chiral Selectors: An Overview -- High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview -- Application of Sub-2 Micron Particles Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography -- Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview -- Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand-Exchange -- Applications of Chiral Supercritical Fluid Chromatography -- Chiral Separations by Countercurrent Chromatography -- Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations -- Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations -- Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer -- Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins -- Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes -- Chiral Capillary Electrophoresis-Mass Spectrometry -- Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography -- Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry -- Application of a (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography -- Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview
    Abstract: This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry
    Pages: XIV, 487 p. 147 illus., 57 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493994380
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  • 53
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: 1. Classification of protein disulfide bonds -- 2. Assessing the evolutionary conservation of protein disulphide bonds -- 3. A proteomics workflow for the Identification of labile disulphide bonds at the cell surface -- 4. Quantification of the redox state of protein disulfide bonds -- 5. Quantification of the redox state of protein disulfide bonds -- 6. Studying functional disulfide bonds by computer simulations -- 7. Dynamic force spectroscopy analysis on the redox states of protein disulfide bonds -- 8. Assays of thiol isomerase enzymatic activity -- 9. Functional assays of thiol isomerase ERp5 -- 10. IDENTIFICATION OF PDI SUBSTRATES BY MECHANISM-BASED KINETIC TRAPPING -- 11. Site-specific proteomic mapping of modified cysteine residues -- 12. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry -- 13. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry -- 14. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide -- 15. Methodology for identification of the cysteine-reactive covalent inhibitors -- 16. In Vivo Measurement of Redox-Regulated TG2 Activity -- 17. Quantitation of total and free thiol β2-glycoprotein I levels for diagnostic and prognostic purposes in the antiphospholipid syndrome -- 18. Quantitation of total and free thiol angiotensinogen as a prognostic marker for pre-eclampsia -- 19. Preparation of a dithiol-reactive probe for PET imaging of cell death -- 20. Flow Cytometry Assessment of Procoagulant Platelets Using A Dithiol Reactive Probe
    Abstract: This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study
    Pages: XI, 327 p. 124 illus., 69 illus. in color. : online resource.
    ISBN: 9781493991877
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  • 54
    Keywords: Toxicology ; Psychopharmacology ; Neurosciences ; Pharmacology/Toxicology ; Psychopharmacology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Part 1: Overview and General Principles -- 1. Impact, Diagnosis, Phenomenology, and Biology -- Part 2: The Past – from antiquity to 1970 including TCAs and MAOIs -- 2. Tricyclic Antidepressants and Monoamine Oxidase Inhibitors: Are They Too Old for a New Look? -- Part 3: The Present- 1970 to 2016 -- 3. Clinical Implications of the STAR*D Trial -- 4. Pharmacogenomics and Biomarkers of Depression -- 5. Therapeutic Drug Monitoring of Antidepressants -- 6. Selective Serotonin Reuptake Inhibitors -- 7. Serotonin and Norepinephrine Reuptake Inhibitors -- 8. Neurostimulation Therapies -- 9. Current Role of Herbal and Natural Preparations -- Part 4: The future -- 10. Role of Inflammation in Depression and Treatment Implications -- 11. NMDA Antagonists for Treatment-Resistant Depression -- 12. Drug Development in Psychiatry: The Long and Winding Road from Chance Discovery to Rational Development -- 13. Other Antidepressants -- Part 5: Use of Antidepressants in Special Populations -- 14. Use of Antidepressants in Patients with Co-occurring Depression and Substance Use Disorders -- 15. Treatment of Depression in Women -- 16. Management of Late-Life Depression -- 17. The Use of Antidepressants in Bipolar Depression
    Abstract: This volume reviews the known neurobiology of depression and combines classic data on antidepressant treatments with modern theory on the physiology of depression. It also discusses novel mechanism of action drugs
    Pages: XII, 442 p. 17 illus., 8 illus. in color. : online resource.
    ISBN: 9783030109493
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