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  • Springer eBooks  (3,540)
  • Neurologie
  • Theoretische Physik
  • Messtechnik
  • Strömungsmechanik
  • Springer  (3,574)
  • American Institute of Physics (AIP)
  • 1
    Keywords: Neurology ; Neurobiology ; Neurology ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Integrative Biomarkers in Stroke -- Vascular-Related Biomarkers of Ischemic Stroke -- Generation and Applicability of Genetic Risk Scores (GRS) in Stroke -- Neuroplasticity Biomarkers in Experimental Stroke Recovery -- Methods of Mitochondrial and Redox Measurements in Ischemic Stroke -- Blood Biomarkers for Stroke Differentiation -- Laser-Capture Micro-Dissection for Measurement of Angiogenesis after Stroke -- Blood-Borne Biomarkers of Hypertension Predicting Hemorrhagic and Ischemic Stroke -- RNA Gene Expression to Identify the Etiology of Acute Ischemic Stroke: The Biomarkers of Acute Stroke Etiology (BASE) Study -- Neurotoxicity Biomarkers Assays Development -- Glutamate Receptor Peptides as Potential Neurovascular Biomarkers of Acute Stroke -- Antibodies to NMDA Receptors in Cerebral and Spinal Cord Infarctions -- Impaired Retinal Vasoreactivity as an Early Marker of Stroke Risk in Diabetes -- Imaging Biomarkers: Keys to Decision Making in Stroke -- Neuroimaging Methods for Acute Stroke Diagnosis and Treatment -- Ultrasound Assessments of Risk for TIA and Stroke in Vascular Surgery -- Preoperative and Intraoperative Markers of Cerebral Ischemia -- Time is Brain: the Prehospital Phase and the Mobile Stroke Unit -- Acute Clinical Intervention and Chronic Management of Cerebral Vascular Accident -- Trends in Biomarkers Development for Stroke
    Abstract: This volume presents the latest data on recent achievements in new and emerging technologies for stroke biomarkers and innovations in stroke assessment. The topics discussed in this book explore the role of upcoming biomarkers in different types of stroke, and explores techniques that will allow researchers to be more effective when approaching clinical management and patient care. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to ensure successful results in the laboratory. Cutting-edge and comprehensive, Stroke Biomarkers is a valuable resource for both experimental and clinical scientists interested in expanding their knowledge in the field of stroke research
    Pages: XVII, 425 p. 80 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493996827
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  • 2
    Keywords: Neurobiology ; Developmental Biology ; Neurosciences ; Laboratory animals ; Neurobiology ; Developmental Biology ; Neurosciences ; Animal Models ; Springer eBooks
    Description / Table of Contents: Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra -- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis -- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis -- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System -- Immunostaining of the Embryonic and Larval Drosophila Brain -- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila -- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling -- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update -- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain -- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila -- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development -- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum -- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium -- X-Ray Microscopy of the Larval Crustacean Brain -- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain -- Immunostainings in Nervous System Development of the Nematode C. elegans -- Methods in Brain Development of Molluscs -- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization -- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum -- In Situ Hybridization and Immunostaining of Xenopus Brain -- Morpholino Studies in Xenopus Brain Development -- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish -- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish -- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain -- Gene Silencing in Chicken Brain Development -- Transplantation of Neural Tissue: Quail-Chick Chimeras -- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development -- The Cre/Lox System to Assess the Development of the Mouse Brain -- In Utero Electroporation to Study Mouse Brain Development
    Abstract: This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain
    Pages: XIV, 527 p. 116 illus., 96 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493997329
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  • 3
    Keywords: Infectious Diseases ; Immunology ; Infectious Diseases ; Immunology ; Springer eBooks
    Description / Table of Contents: Tour de Herpes: Cycling through the Life and Biology of HSV-1 -- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology -- Herpes Simplex Virus Growth, Preparation, and Assay -- Engineering HSV-1 Vectors for Gene Therapy -- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- oHSV Genome Editing by Means of galK Recombineering -- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein -- CRISPR/Cas9-Based Genome Editing of HSV -- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH) -- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing -- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals -- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry -- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells -- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot -- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane -- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination -- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1 -- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
    Abstract: This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV
    Pages: XIV, 457 p. 60 illus., 45 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998142
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  • 4
    Keywords: Toxicology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels
    Abstract: This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 293 p. 46 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998456
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  • 5
    Keywords: Bacteriology ; Emerging infectious diseases ; Bacteriology ; Infectious Diseases ; Springer eBooks
    Description / Table of Contents: Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains -- Spa Genotyping of Staphylococcus aureus Isolates -- Multilocus Sequence Typing of Staphylococcus aureus -- Genetic Manipulations of Staphylococcal Chromosomal DNA -- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology -- RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus -- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus -- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology -- Metabolomic Profiling of Staphylococcus aureus -- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells -- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging -- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options -- Animal Models for Drug Development for MRSA
    Abstract: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens
    Pages: XI, 268 p. 35 illus., 27 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998494
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  • 6
    Keywords: Biomedical Engineering ; Cell Biology ; Bioinformatics ; Biology—Technique ; Systems Biology ; Biological systems ; Biomedical Engineering/Biotechnology ; Cell Biology ; Computational Biology/Bioinformatics ; Biological Techniques ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB: Image Analysis & Brownian Motion -- Resolving the process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM)
    Abstract: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images
    Pages: X, 170 p. 69 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030223861
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  • 7
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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  • 8
    Keywords: Microbial genetics ; Microbial genomics ; Bacteriology ; Microbial Genetics and Genomics ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples -- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health -- Detection, Isolation, and Characterization of Plasmids in the Environment -- Bacteriophage Isolation and Characterization: Phages of Escherichia coli -- Detection and Characterization of Transposons in Bacteria -- Measuring Plasmid Conjugation Using Antibiotic Selection -- Measuring Plasmid Conjugation Using Fluorescent Reporters -- Methods to Quantify DNA Transfer in Enterococcus -- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis -- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases -- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis -- First Biochemical Steps on Bacterial Transposition Pathways -- Natural Transformation in Escherichia coli -- Integron Identification in Bacterial Genomes and Cassette Recombination Assays -- Methods to Identify and Analyze Vesicle-Protected DNA Transfer -- Measuring Plasmid Stability in Gram-Negative Bacteria -- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems -- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements -- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan -- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS) -- MOBscan: Automated Annotation of MOB Relaxases -- Plasmid Typing and Classification -- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets -- Statistical Analysis of Accessory Genome -- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits -- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria -- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria -- Conjugative Assembly Genome Engineering (CAGE)
    Abstract: This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and transduction in HGT as well as a series of methods to analyze the adaptation and evolution of MGEs, with special attention paid to bioinformatics tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Horizontal Gene Transfer: Methods and Protocols serves as an ideal guide to the further study of this pervasive, all-important mechanism of genetic originality
    Pages: XV, 413 p. 62 illus., 52 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998777
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  • 9
    Keywords: Human Genetics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Experimenting with Trinucleotide Repeats: Facts and Technical Issues -- Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions -- Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae -- Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis -- Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae -- Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells -- Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture -- The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model -- In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA -- FISH Protocol for Myotonic Dystrophy Type 1 Cells -- Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases -- Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia -- GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats -- Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Abstract: This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study
    Pages: XI, 286 p. 65 illus., 53 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997848
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  • 10
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures
    Abstract: This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research
    Pages: XV, 363 p. 72 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493976171
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  • 11
    Keywords: Mass spectrometry ; Proteomics ; Mass spectrometry ; Proteomics ; Springer eBooks
    Description / Table of Contents: Introduction to Mass Spectrometry-based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Comparing Peptide Spectra Matches Across Search Engines -- Calculation of False Discovery Rate for Peptide and Protein Identification -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Interpretation of Tandem Mass Spectra of Posttranslationally Modified Peptides -- Solution to Dark Matter Identified by Mass-tolerant Database Search -- Phosphoproteomics Profiling to Identify Altered Signaling Pathways and Kinase-targeted Cancer Therapies -- Mass Spectrometry Based Characterization of Ub- and UbL-modified Proteins -- Targeted Proteomics as a Tool for Quantifying Urine-based Biomarkers -- Data Imputation in Merged Isobaric Labelling-based Relative Quantification Datasets -- Clustering Clinical Data in R -- Review of Issues and Solutions to Data Analysis Reproducibility and Data Quality in Clinical Proteomics -- Review of Batch Effects Prevention, Diagnostics, and Correction Approaches -- Using the Object-oriented PowerShell for Simple Proteomics Data Analysis -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data
    Abstract: The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics. In contrast to the previous editions, this third edition aims to provide the most relevant computational methods, focusing on computational concepts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Data Analysis in Proteomics, Third Edition to ensure successful results in the further study of this vital field
    Pages: X, 445 p. 136 illus., 94 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493997442
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  • 12
    Unknown
    New York, NY : Springer
    Keywords: Neurosciences ; Anesthesiology ; Neurosciences ; Anesthesiology ; Springer eBooks
    Description / Table of Contents: The Challenge of Accidental Awareness during General Anesthesia -- Impact of Anesthetics on Brain Electrical Activity and Principles of pEEG-Based Monitoring during General Anethesia -- Perioperative Monitoring of Autonomic Nervous Activity -- Monitoring Cerebral Oximetry by Near Infrared Spectroscopy (NIRS) in Anaesthesia and Critical Care: Progress and Perspectives -- Post-Traumatic Stress Disorder following Intraoperative Awareness -- Mechanisms of Action of Inhaled Volatile General Anesthetics: Unconsciousness at the Molecular Level -- Intravenous Hypnotic Agents: From Binding Sites to Loss of Consciousness -- Pharmacological Considerations for the Use of General Anesthetics in the Elderly -- Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies -- Postoperative Cognitive Function following General Anesthesia in Children -- The Challenge of Opioid-Free Anesthesia -- New Insights into the Pharmacology of Dexmedetomidine and Open Issues for Neurosurgical Procedures -- Transgenic Mouse Models, General Anesthetics and Alzheimer Disease: Findings from Preclinical Studies -- The Biochemical Basis of Delirium -- Postoperative Delirium and Postoperative Cognitive Dysfunction
    Abstract: This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology. Furthermore, chapters in this book cover technical topics such as preoperative monitoring of autonomic nervous activity; EEG based monitoring of general anesthesia; monitoring cerebral oximetry by near infrared spectroscopy; pharmacological issues of anesthesia interest; and optimization of perioperative course through the latest techniques such as the opioid-free anesthesia. Finally, this book offers a comprehensive and up-to-date discussion on the phenomenon of general anesthesia awareness and its potential psychological sequelae. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, General Anesthesia Research is a valuable resource for novice and expert anesthesiologists who want to learn more about the important advances made in this developing field
    Pages: XIV, 256 p. 15 illus., 14 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998913
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  • 13
    Keywords: Antibodies ; Pharmacology ; Antibodies ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: An Overview of the Current ADC Discovery Landscape -- Pushing the Envelope: Advancement of ADCs Outside of Oncology -- Conjugations to Endogenous Cysteine Residues -- Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies -- Transglutaminase-Mediated Conjugations -- Click Chemistry Conjugations -- Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates -- Bridged Cysteine Conjugations -- Antibody Conjugations via Glycosyl Remodeling -- ADC Analysis by Hydrophobic Interaction Chromatography -- Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs -- Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction -- Analysis of ADCs by Native Mass Spectrometry -- High Resolution Characterization of ADCs by Orbitrap LCMS -- Conjugation Site Analysis by MS/MS Protein Sequencing -- Conjugation Site Analysis of Lysine-Conjugated ADCs -- Characterization of ADCs by Capillary Electrophoresis -- Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry -- Purification of ADCs by Hydrophobic Interaction Chromatography -- Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations -- Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions -- Biophysical Methods for Characterization of Antibody Drug Conjugates -- Determination of ADC Cytotoxicity in Immortalized Human Cell Lines -- LC/MS Methods for Studying Lysosomal ADC Catabolism -- Assessing ADC Plasma Stability by LC/MS Methods -- Determination of ADC Concentration by Ligand-Binding Assays
    Abstract: This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the €œáctivation barrieŕ€ when undertaking a new discipline, and provides a ́€œtoolbox́€ for the next generation of ADC scientists
    Pages: XI, 373 p. 138 illus., 109 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493999293
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  • 14
    Keywords: Molecular Biology ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? -- Practical Considerations for In Vivo Studies -- Nutritional Models of Type 2 Diabetes Mellitus -- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes -- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture -- Gene Expression Mining in Type 2 Diabetes Research -- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes -- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry -- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation -- Quantification of Pancreatic Islets: Using Image Analysis Tools -- The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy -- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells -- Primary Adipocytes as a Model for Insulin Sensitivity -- Assessing Islet Transplantation Outcome in Mice -- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent
    Abstract: This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field
    Pages: X, 300 p. 50 illus., 32 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998821
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  • 15
    Keywords: Human Genetics ; Proteins  ; Human Genetics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies
    Abstract: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field
    Pages: XII, 445 p. 86 illus., 61 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998531
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  • 16
    Keywords: Botany ; Plant genetics ; Plant Sciences ; Plant Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
    Abstract: The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
    Pages: XI, 247 p. 30 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998654
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  • 17
    Keywords: Immunology ; Cytology ; Immunology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook -- Immunophenotyping by Mass Cytometry -- Fluorescent Cell Barcoding for Immunophenotyping -- Immunophenotyping using Dried and Lyophilized Reagents -- Guidelines for Gating Flow Cytometry Data for Immunological Assays -- Strategies and Techniques for NK Cell Phenotyping -- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue -- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells -- Immunophenotyping of Human Regulatory T Cells -- Immunophenotyping of Human Innate Lymphoid Cells -- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis -- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles -- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells -- Quality Control of Immunophenotyping -- Immunophenotyping of Acute Myeloid Leukemia -- Immunophenotyping of Acute Lymphoblastic Leukemia -- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia -- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
    Abstract: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field
    Pages: X, 356 p. 94 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493996506
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  • 18
    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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  • 19
    Keywords: Cell Biology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Introduction to Purinergic Signaling -- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling -- Agonists and Antagonists for Purinergic Receptors -- Homology Modelling of P2X Receptors -- Using RNA Interference for Purinoceptor Knock Down in vivo -- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos -- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues -- Flow Cytometry of Membrane Purinoreceptors -- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation -- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking -- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer -- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy -- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor -- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse -- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging -- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels -- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader -- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes -- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells -- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages -- Automated Planar Patch-clamp Recording of P2X Receptors -- Controlling Engineered P2X Receptors with Light -- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment -- Assays to Measure Purinoceptor Pore Dilation -- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization -- Measuring Leukocyte Migration to Nucleotides -- Assessment of Cell Adhesion after Purinoceptor Activation
    Abstract: This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines
    Pages: XII, 360 p. 82 illus., 49 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997176
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  • 20
    Keywords: Proteins  ; Biotechnology ; Nanotechnology ; Protein Science ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Introduction to Protein Nanotechnology -- Protein Expression in the Baculovirus-Insect Cell Expression System -- Peroxiredoxin Proteins as Building Blocks for Nanotechnology -- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins -- ATP Synthase: Expression, Purification, and Function -- Molecular Superglues: Discovery and Engineering Orthogonalization -- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes -- Adding Function to Protein Scaffolds -- Virus-Derived Nanoparticles -- Strategies for Increasing Protein Stability -- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol -- Nanotechnology with S-Layer Proteins -- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy -- Atomic Force Microscopy of Proteins -- Native Protein Mass Spectrometry -- Nanoparticle Tracking Analysis of b-Casein Nanocarriers -- Molecular Dynamics Simulation of Proteins
    Abstract: This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field
    Pages: XIII, 331 p. 84 illus., 64 illus. in color. : online resource.
    Edition: 3rd ed. 2020.
    ISBN: 9781493998692
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  • 21
    Keywords: Biochemistry ; RNA-ligand interactions ; Protein Science ; Protein-Ligand Interactions ; Springer eBooks
    Description / Table of Contents: Predicting Protein-Protein Interactions using SPRINT -- Automated Extraction and Visualization of Protein-Protein Interaction Networks and Beyond: A Text Mining Protocol -- Construction of Functional Protein Networks using Domain Profile Associations -- Reconstruction of Protein-Protein Interaction Networks using Homology-Based Search: Application to the Autophagy Pathway of Ageing in Podospora anserina -- Predicting Interacting Protein Pairs by Co-Evolutionary Paralog Matching -- A Web-Based Protocol for Inter-Protein Contact Prediction by Deep Learning -- Visual Analysis of Protein-Protein Interaction Docking Models using COZOID Tool -- Path-LZerD: Predicting Assembly Order of Multimeric Protein Complexes -- Embedding Alternative Conformations of Proteins in Protein-Protein Interaction Networks -- Informed Use of Protein-Protein Interaction Data: A Focus on the Integrated Interactions Database (IID) -- Generation and Interpretation of Context-Specific Human Protein-Protein Interaction Networks with HIPPIE -- Explore Protein-Protein Interactions for Cancer Target Discovery using the OncoPPi Portal -- Perform Pathway Enrichment Analysis using ReactomeFIViz -- De Novo Pathway Enrichment with KeyPathwayMiner -- De Novo Pathway-Based Classification of Breast Cancer Subtypes -- Vienna Graph Clustering -- On TD-WGcluster - Theoretical Foundations and Guidelines for the User -- An Introductory Guide to Aligning Networks using SANA, the Simulated Annealing Network Aligner
    Abstract: This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field
    Pages: XI, 286 p. 97 illus., 79 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998739
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  • 22
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example
    Abstract: This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field
    Pages: XIV, 405 p. 76 illus., 66 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998180
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  • 23
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 24
    Keywords: Immunology ; Medical virology ; Immunology ; Virology ; Springer eBooks
    Description / Table of Contents: Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus -- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV -- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR) -- Establishment of Replication-competent HCV Strain with Minimum Modifications -- Full-length Open Reading Frame Amplification of Hepatitis C Virus -- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing -- A Cell Culture Model for Persistent HCV Infection -- Expression Cloning of Host Factors Required for the HCV Replication Cycle -- Application of Deep Mutational Scanning in Hepatitis C Virus -- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals -- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay -- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells -- Functional Characterization of Apolipoproteins in the HCV Life Cycle -- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes -- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins -- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis -- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells -- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact -- Complement Regulation and Immune Evasion by Hepatitis C Virus -- Direct ex vivo Functional Analysis of HCV-specific T Cells -- ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry -- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display -- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA -- In vitro Neutralization Assay using Cultured Hepatitis C Virus -- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp) -- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice -- Mice with Chimeric Human Livers and Their Applications -- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies
    Abstract: This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field
    Pages: XIX, 508 p. 75 illus., 47 illus. in color. : online resource.
    ISBN: 9781493989768
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  • 25
    Keywords: Toxicology ; Cytology ; Invertebrates ; Pharmacology/Toxicology ; Cell Biology ; Invertebrates ; Springer eBooks
    Description / Table of Contents: Protective Responses of Different Organs to Environmental Toxicants or Stresses -- Avoidance Behaviour of Nematodes to Environmental Toxicants or Stresses -- Intestinal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Epidermal Barrier for Nematodes against Toxicity of Environmental Toxicants or Stresses -- Toxicity Induction in Intestine and Epidermis in Nematodes Exposed to Environmental Toxicants or Stresses -- Toxicity Induction in Neurons and Muscle in Nematodes Exposed to Environmental Toxicants or Stresses -- Reproductive Toxicity Induction in Nematodes Exposed to Environmental Toxicants or Stresses -- Intestinal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Epidermal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Neuronal Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses -- Germline Signalling Pathways Required for the Regulation of Toxicity of Environmental Toxicants or Stresses
    Abstract: This book introduces readers to intestinal and epidermal barriers, and to toxicity induction of environmental toxicants or stresses in the intestine, epidermis, neurons, muscle, and reproductive organs in Caenorhabditis elegans. In addition, it discusses the protective responses of various organs and nematodes’ avoidance behaviour with regard to environmental toxicants or stresses. The intestinal, epidermal, neuronal, and germline signalling pathways required for the regulation of toxicity of environmental toxicants or stresses are also introduced and discussed. As a classic model animal with well-described genetic and developmental backgrounds, the nematode Caenorhabditis elegans has been successfully and widely used in both toxicity assessments and toxicological studies on various environmental toxicants and stresses. Once exposure to certain environmental toxicants has occurred, the toxicants can enter into the primary targeted organs (such as intestinal cells), and even be translocated into secondary targeted organs (such as reproductive organs and neurons). Based on related available data, this book provides a systematic understanding of target organ toxicology in C. elegans
    Pages: XIII, 337 p. 232 illus., 175 illus. in color. : online resource.
    ISBN: 9789811360107
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  • 26
    Keywords: Medicine ; Artificial intelligence ; Algorithms ; Biomedicine general ; Artificial intelligence ; Algorithms ; Springer eBooks
    Description / Table of Contents: Preface -- Introduction to Deep Density Models with Latent Variables -- Deep RNN Architecture: Design and Evaluation -- Deep Learning Based Handwritten Chinese Character and Text Recognition -- Deep Learning and Its Applications to Natural Language Processing -- Deep Learning for Natural Language Processing -- Oceanic Data Analysis with Deep Learning Models -- Index
    Abstract: The purpose of this edited volume is to provide a comprehensive overview on the fundamentals of deep learning, introduce the widely-used learning architectures and algorithms, present its latest theoretical progress, discuss the most popular deep learning platforms and data sets, and describe how many deep learning methodologies have brought great breakthroughs in various applications of text, image, video, speech and audio processing. Deep learning (DL) has been widely considered as the next generation of machine learning methodology. DL attracts much attention and also achieves great success in pattern recognition, computer vision, data mining, and knowledge discovery due to its great capability in learning high-level abstract features from vast amount of data. This new book will not only attempt to provide a general roadmap or guidance to the current deep learning methodologies, but also present the challenges and envision new perspectives which may lead to further breakthroughs in this field. This book will serve as a useful reference for senior (undergraduate or graduate) students in computer science, statistics, electrical engineering, as well as others interested in studying or exploring the potential of exploiting deep learning algorithms. It will also be of special interest to researchers in the area of AI, pattern recognition, machine learning and related areas, alongside engineers interested in applying deep learning models in existing or new practical applications
    Pages: VII, 163 p. 66 illus., 46 illus. in color. : online resource.
    ISBN: 9783030060732
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  • 27
    Keywords: Human Genetics ; Metabolic Diseases ; Pediatrics ; Medicine ; Human Genetics ; Metabolic Diseases ; Pediatrics ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Normal Growth in PKU Patients Under Low-Protein Diet in a Single-Center Cross-Sectional Study -- Serial Magnetic Resonance Imaging and 1H-Magnetic Resonance Spectroscopy in GABA Transaminase Deficiency: A Case Report.-Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment -- Cognitive and Behavioural Outcomes of Paediatric Liver Transplantation for Ornithine Transcarbamylase Deficiency -- Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation -- Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice -- Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period -- Severe Leukoencephalopathy with Clinical Recovery Caused by Recessive BOLA3 Mutations -- Neonatal Onset Interstitial Lung Disease as a Primary Presenting Manifestation of Mucopolysaccharidosis Type I -- A Middle Eastern Founder Mutation Expands the Genotypic and Phenotypic Spectrum of Mitochondrial MICU1 Deficiency: A Report of 13 Patients -- Disruption of the Responsible Gene in a Phosphoglucomutase 1 Deficiency Patient by Homozygous Chromosomal Inversion -- Evaluation of Disease Lesions in the Developing Canine MPS IIIA Brain -- Extrapolation of Variant Phase in Mitochondrial Short-Chain Enoyl-CoA Hydratase (ECHS1) Deficiency -- RFT1-CDG: Absence of Epilepsy and Deafness in Two Patients with Novel Pathogenic Variants -- Short-Term Administration of Mycophenolate Is Well-Tolerated in CLN3 Disease (Juvenile Neuronal Ceroid Lipofuscinosis)
    Abstract: JIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder
    Pages: VI, 124 p. 35 illus., 20 illus. in color. : online resource.
    ISBN: 9783662586143
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  • 28
    Keywords: Plant genetics ; Gene Expression ; Plant Genetics and Genomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants -- The Plant MicroRNA Pathway: The Production and Action Stages -- Plant miRNA Conservation and Evolution -- Bioinformatic Analysis of Small RNA Sequencing Libraries -- An Overview of miRNA and miRNA Target Analysis Tools -- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences -- Prediction of Plant miRNA Targets -- Isolation and Detection Methods of Plant miRNAs -- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants -- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses -- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization -- In Situ Localization of Small RNAs in Plants -- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs -- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy -- Degradome Sequencing in Plants -- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System -- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis -- Mimicry Technology: A Versatile Tool for Small RNA Suppression -- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants -- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches -- Analysis of Methylation Status of Plant MicroRNAs -- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 -- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs -- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae -- Detection of Histone Modifications Associated with miRNAs
    Abstract: This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science
    Pages: XII, 363 p. 70 illus., 48 illus. in color. : online resource.
    ISBN: 9781493990429
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  • 29
    Keywords: Cytology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction -- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction -- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells -- Isolation and Characterization of Cutaneous Epithelial Stem Cells -- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin -- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells -- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis -- Isolation and Cultivation of Epidermal (Stem) Cells -- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen -- Isolation and Cultivation of Skin-Derived Precursors -- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells -- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor -- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair -- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet -- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering -- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration -- Skin Wound Healing: Refractory Wounds and Novel Solutions -- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice -- Melanoblasts as Multipotent Cells in Murine Skin -- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro -- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells -- Isolating Immune Cells from Mouse Embryonic Skin -- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells -- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy -- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications -- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis -- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells -- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research -- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma -- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells -- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells
    Abstract: This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells
    Pages: XV, 463 p. 105 illus., 76 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493988709
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  • 30
    Keywords: Oncology ; Immunology ; Cancer Research ; Immunology ; Springer eBooks
    Description / Table of Contents: Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry
    Abstract: This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies
    Pages: XI, 225 p. 50 illus., 35 illus. in color. : online resource.
    ISBN: 9781493989799
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  • 31
    Keywords: Neurosciences ; Cytology ; Neurosciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse -- Role of Astrocytes in the Neurogenic Niches -- Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains -- Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon -- A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ -- A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples -- Cisterna Magna Injection in Rats to Study Glymphatic Function -- Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface -- Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission -- Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System -- In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes -- Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ -- Imaging of Local and Global Sodium Signals in Astrocytes -- Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator -- Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes -- Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury -- Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain -- Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder -- A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque -- Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes
    Abstract: This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells
    Pages: XI, 295 p. 66 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990689
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  • 32
    Keywords: Bioinformatics ; Human Genetics ; Bioinformatics ; Human Genetics ; Computational Biology/Bioinformatics ; Springer eBooks
    Description / Table of Contents: ncRNAs in Inflammatory and Infectious Diseases -- p73-Governed miRNA Networks: Translating Bioinformatics Approaches to Therapeutic Solutions for Cancer Metastasis -- Methods for Annotation and Validation of Circular RNAs from RNAseq Data -- Methods to Study Long Non-coding RNA Expression and Dynamics in Zebrafish using RNA Sequencing -- Workflow Development for the Functional Characterization of ncRNAs -- ncRNA Editing: Functional Characterization and Computational Resources -- Computational Prediction of Functional microRNA – mRNA Interactions -- Tools for Understanding miRNA-mRNA Interactions for Reproducible RNA Analysis -- Computational Resources for Prediction and Analysis of Functional miRNA and their Targetome -- Non-coding RNAs Databases: Current Status and Trends -- Controllability Methods for Identifying Associations between Critical Control ncRNAs and Human Diseases -- Network-based Methods and Other Approaches for Predicting lncRNA Functions and Disease Associations -- Integration of miRNA and mRNA Expression Data for Understanding Etiology of Gynecologic Cancers -- Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks -- Kinetic Modeling of Competition and Depletion of Shared miRNAs by Competing Endogenous RNAs -- Modeling ncRNA-mediated Circuits in Cell Fate Decision -- Modeling Long ncRNA-mediated Regulation in the Mammalian Cell Cycle
    Abstract: This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field
    Pages: XII, 451 p. 78 illus., 65 illus. in color. : online resource.
    ISBN: 9781493989829
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  • 33
    Keywords: Pharmaceutical technology ; Chromatography ; Chemical engineering ; Pharmaceutical Sciences/Technology ; Chromatography ; Industrial Chemistry/Chemical Engineering ; Springer eBooks
    Description / Table of Contents: Separation and Purification of Amino acids -- Ion exchange chromatography for enzyme immobilization -- Determination of morphine in urine -- Chromatographic Separation of Amino Acids -- Applications of ion exchange chromatography in pharmaceutical analysis -- Thermodynamic Kinetics and Sorption of Bovine Serum Albumin with Different Clay materials -- Sorbitol demineralization by ion exchange -- Separation and purification of nucleotides, nucleosides, purine and pyrimidine bases by ion exchange -- Separation and purification of vitamins: vitamin B1, B2, B6, C, and K1 -- Colour removal from sugar syrups
    Abstract: This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists
    Pages: XII, 225 p. 41 illus., 29 illus. in color. : online resource.
    ISBN: 9783030060824
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  • 34
    Keywords: Medical laboratories ; Pharmaceutical technology ; Laboratory Medicine ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Part1.Introduction -- 1.1 Rules of laboratory safety in a pharmacology laboratory -- 1.2 Commom instruments and their uses used in a pharmacology laboratory -- Part2.General aspects of Pharmacology laboratory -- 2.1 Safety and Risk assessment -- 2.2 Use and Handling of laboratory animals -- 2.3 Experimental design -- 2.4 Essential statistics -- 2.5 Cumulative Dose Response Curve -- 2.6 Toxicology -- 2.7 Basic instruments and techniques in Pharmacology Laboratory -- Part3.Isolated tissues and organs -- 3.1 Basic instruments used for isolated tissue experiments -- 3.2 Organ baths -- 3.3 Smooth muscle preparations -- 3.4 Skeletal muscle preparations -- 3.5 Cardiac muscle preparations -- Part4.Isolated tissues for screening of drugs -- 4.1 Evaluation of antidiabetic agents -- 4.2 Evaluation of antidepressants -- 4.3 Evaluation of antihypertensive agents -- 4.4 Evaluation of antiulcer agents -- 4.5 Evaluation of hepatotoxicity -- 4.6 Evaluation of Antioxidant agents -- 4.7 Evaluation of local anaesthetics -- Part5.Genotoxicity and Toxicological studies -- 5.1 The Mouse Lymphoma Assay -- 5.2 The Comet Assay -- 5.3 In vitro Genotoxicity assay -- 5.4 In vitro Teratogenicity Testing -- 5.5 Histopathological studies of animal tissues -- 5.6 Drug poisoning -- Part6.Experimental Animal studies -- 6.1 Pyrogen testing -- 6.2 Collecting blood from mice -- 6.3 Studies on different parameters of blood -- 6.4 Experiment on central nervous system -- 6.5 Evaluation on cardiovascular system -- 6.6 Experiments on GI tract -- Part7.Clinical trials -- 7.1 Clinical Pharmacology and its genesis -- 7.2 National and International agencies and their role in Clinical pharmacology -- 7.3 Stages in drug development and clinical trials -- 7.4 Ethics in Clinical research -- 7.5 Safety assessment in Clinical trials -- Part8.IPR and ethics in animal studies -- 8.1 Intellectual property rights and its different categories -- 8.2 Importance of IPR in drug development -- 8.3 Patenting cells, cell lines and animals -- 8.4 Ethics in laboratory animal studies -- 8.5 Risk assessment and management -- 8.6 Good laboratory practices
    Abstract: Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This manual is the textbook covering the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory
    Pages: XIII, 142 p. 50 illus., 39 illus. in color. : online resource.
    ISBN: 9789811363559
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  • 35
    Keywords: Otorhinolaryngology ; Neurosciences ; Otorhinolaryngology ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Preface -- Visual Influence on Auditory Perception -- Cue Combination Within a Bayesian Framework -- Toward a Model of Auditory-Visual Speech Intelligibility -- An Object-Based Interpretation of Audiovisual Processing -- Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway -- Multisensory Processing in the Auditory Cortex -- Audiovisual Integration in the Primate Prefrontal Cortex -- Using Multisensory Integration to Understand the Human Auditory Cortex -- Combining Voice and Face Content in the Primate Temporal Lobe -- Neural Network Dynamics and Audiovisual Integration -- Cross-Modal Learning in the Auditory System -- Multisensory Processing Differences in Individuals with Autism Spectrum Disorder
    Abstract: Auditory behavior, perception, and cognition are all shaped by information from other sensory systems. This volume examines this multi-sensory view of auditory function at levels of analysis ranging from the single neuron to neuroimaging in human clinical populations. Visual Influence on Auditory Perception Adrian K.C. Lee and Mark T. Wallace Cue Combination within a Bayesian Framework David Alais and David Burr Toward a Model of Auditory-Visual Speech Intelligibility Ken W. Grant and Joshua G. W. Bernstein An Object-based Interpretation of Audiovisual Processing Adrian K.C. Lee, Ross K. Maddox, and Jennifer K. Bizley Hearing in a “Moving” Visual World: Coordinate Transformations Along the Auditory Pathway Shawn M. Willett, Jennifer M. Groh, Ross K. Maddox Multisensory Processing in the Auditory Cortex Andrew J. King, Amy Hammond-Kenny, Fernando R. Nodal Audiovisual Integration in the Primate Prefrontal Cortex Bethany Plakke and Lizabeth M. Romanski Using Multisensory Integration to Understand Human Auditory Cortex Michael S. Beauchamp Combining Voice and Face Content in the Primate Temporal Lobe Catherine Perrodin and Christopher I. Petkov Neural Network Dynamics and Audiovisual Integration Julian Keil and Daniel Senkowski Cross-Modal Learning in the Auditory System Patrick Bruns and Brigitte Röder Multisensory Processing Differences in Individuals with Autism Spectrum Disorder Sarah H. Baum Miller, Mark T. Wallace Adrian K.C. Lee is Associate Professor in the Department of Speech & Hearing Sciences and the Institute for Learning and Brain Sciences at the University of Washington, Seattle Mark T. Wallace is the Louise B McGavock Endowed Chair and Professor in the Departments of Hearing and Speech Sciences, Psychiatry, Psychology and Director of the Vanderbilt Brain Institute at Vanderbilt University, Nashville Allison B. Coffin is Associate Professor in the Department of Integrative Physiology and Neuroscience at Washington State University, Vancouver, WA Arthur N. Popper is Professor Emeritus and research professor in the Department of Biology at the University of Maryland, College Park Richard R. Fay is Distinguished Research Professor of Psychology at Loyola University, Chicago
    Pages: XVI, 272 p. 70 illus., 49 illus. in color. : online resource.
    ISBN: 9783030104610
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  • 36
    Keywords: Microbial genetics ; Microbial genomics ; Microbial ecology ; Evolution (Biology) ; Biodiversity ; Drug Interactions ; Microbial Genetics and Genomics ; Microbial ecology ; Evolutionary Biology ; Biodiversity ; Drug Resistance ; Springer eBooks
    Description / Table of Contents: Bacteria -- Functions of archaeal nucleoid proteins: archaeal silencers are still missing -- Acquired and innate immunity in prokaryote define their evolutionary story -- Mobile genetic elements -- Viruses of the archaea and evolution of life -- Overlooked “broad-host range vector particles” (VPs) in the environ -- Bacteria-virus interaction -- Plasmids and their hosts -- Bacterial transposable elements -- RNA-mediated crosstalk between bacterial host genome and foreign genetic elements -- Extracellular DNA in seawater and marine sediment -- Acquiring phenotypic diversification on genomic diversification
    Abstract: This book comprehensively discusses our current understanding of the role and biological mechanisms of horizontal transfer of genetic elements in the environment, which has been important in the evolution of prokaryotes (archaea and bacteria). Horizontal transfer of genetic elements generates variations of prokaryotes and their genomes. Comparative studies of genomes revealed that it frequently occurred during archaeal and bacterial evolution. The book introduces a variety of studies related to horizontal gene transfer, gene silencing, plasmids, phages, transposons, and the emergence of microbes that degrade man-made xenobiotics and have antimicrobial resistance. Written by leading researchers in DNA traffic, the book is a valuable guide to horizontal transfer for both young scientists and experts in the field
    Pages: VII, 278 p. 39 illus., 25 illus. in color. : online resource.
    ISBN: 9789811334115
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  • 37
    Keywords: Human Genetics ; Medical virology ; Human Genetics ; Virology ; Springer eBooks
    Description / Table of Contents: 1. Design of AAV vectors for Delivery of RNAi -- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes -- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer -- 4. Quantitative and Digital Droplet-based AAV Genome Titration -- 5. Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual -- 6. In Situ Hybridization for Detection of AAV-Mediated Gene Expression -- 7. Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina -- 8. Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System -- 9. Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-Transgenic Species -- 10. MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain -- 11. AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection -- 12. Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals -- 13. Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia -- 14. SubILM Injection of AAV for Gene Delivery to the Retina -- 15. Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins -- 16. AAV-Mediated Gene Delivery to the Inner Ear -- 17. Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System -- 18. AAV-Mediated Gene Delivery to Taste Cells of the Tongue -- 19. AAV Vectors for Efficient Gene Delivery to Rodent Hearts -- 20. AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods -- 21 -- AAV-Mediated Gene Delivery to the Lung -- 22. Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration -- 23. rAAV-Mediated Gene Delivery to Adipose Tissue -- 24. AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection
    Abstract: This volume provides a complete and timely guide to the use of adeno-associated virus (AAV) vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease
    Pages: XII, 426 p. 62 illus., 50 illus. in color. : online resource.
    ISBN: 9781493991396
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  • 38
    Keywords: Lipids ; Cell Membranes ; Lipidology ; Membrane Biology ; Springer eBooks
    Description / Table of Contents: 1. Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-stimulated Live Cells -- 2. Monitoring non-vesicular transport of phosphatidylserine and phosphatidylinositol 4-phosphate in intact cells by BRET analysis -- 3. Development of non-specific BRET-based biosensors to monitor plasma membrane inositol lipids in living cells -- 4. Following anterograde transport of phosphatidylserine in yeast in real time -- 5. Imaging lipid metabolism at the Golgi complex -- 6. Advanced in vitro assay system to measure PS and PE transport at ER/mitochondria interface -- 7. Measurement of lipid transport in mitochondria by the MTL complex -- 8. Bi- and trifunctional lipids for visualization of sphingolipid dynamics within the cell -- 9. Indirect lipid transfer protein activity measurements using quantification of glycosphingolipid production -- 10. Measurement of intracellular sterol transport in yeast -- 11. Intracellular and plasma membrane cholesterol labeling and quantification using filipin and GFP-D4 -- 12. Monitoring and modulating intracellular cholesterol trafficking using ALOD4, a cholesterol-binding protein -- 13. Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts -- 14. Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity -- 15. In vitro assays to measure the membrane tethering and lipid transport activities of the Extended Synaptotagmins -- 16. Determining the lipid binding specificity of SMP domains: an ERMES subunit as a case study -- 17. In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members -- 18. Purification and Characterization of Human Niemann–Pick C1 Protein -- 19. In vitro strategy to measure sterol/phosphatidylinositol 4-phosphate exchange between membranes -- 20. Determination of Ligand Binding Affinity and Specificity of Purified START Domains by Thermal Shift Assays Using Circular Dichoism -- 21. Synthesis of Fluorescent Membrane-spanning Lipids for Studies of Lipid Transfer and Membrane-Fusion -- 22. Setting-up all-atom molecular dynamics simulations to study the interactions of peripheral membrane proteins with model lipid bilayers
    Abstract: This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Intracellular Lipid Transport: Methods and Protocols serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process
    Pages: XI, 345 p. 70 illus., 56 illus. in color. : online resource.
    ISBN: 9781493991365
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  • 39
    Keywords: Bioinformatics ; Cytology ; Bioinformatics ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Quality Control of Single-cell RNA-seq -- Normalization for Single-cell RNA-seq Data Analysis -- Analysis of Technical and Biological Variability in Single-cell RNA Sequencing -- Identification of Cell Types from Single-cell Transcriptomic Data -- Rare Cell Type Detection -- scMCA- A Tool Defines Cell Types in Mouse Based on Single-cell Digital Expression -- Differential Pathway Analysis -- Differential Pathway Analysis -- Estimating Differentiation Potency of Single Cells using Single Cell Entropy (SCENT) -- Inference of Gene Co-expression Networks from Single-Cell RNA-sequencing Data -- Single-cell Allele-specific Gene Expression Analysis -- Using BRIE to Detect and Analyse Splicing Isoforms in scRNA-seq Data -- Preprocessing and Computational Analysis of Single-cell Epigenomic Datasets -- Experimental and Computational Approaches for Single-cell Enhancer Perturbation Assay -- Antigen Receptor Sequence Reconstruction and Clonality Inference from scRNA-seq Data -- A Hidden Markov Random Field Model for Detecting Domain Organizations from Spatial Transcriptomic Data
    Abstract: This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis
    Pages: X, 271 p. 168 illus., 156 illus. in color. : online resource.
    ISBN: 9781493990573
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  • 40
    Keywords: Biotechnology ; Biochemistry ; Biotechnology ; Protein Science ; Springer eBooks
    Description / Table of Contents: Established and Upcoming Yeast Expression Systems -- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts -- Pichia pastoris Promoters -- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes -- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha -- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica -- High-throughput Screening and Selection of Pichia pastoris Strains -- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke -- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori -- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae -- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast -- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production -- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments -- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris -- Efficient Development of a Mixed Feed Process for Pichia pastoris -- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification -- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants -- LC-MS analysis of (glyco)-proteins of Pichia pastoris -- Assessing the Quality of Recombinant Products Made in Yeast -- Quantification of Recombinant Products In Yeast
    Abstract: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications
    Pages: XII, 431 p. 60 illus., 46 illus. in color. : online resource.
    ISBN: 9781493990245
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  • 41
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism
    Abstract: This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories
    Pages: XI, 495 p. 79 illus., 60 illus. in color. : online resource.
    ISBN: 9781493990276
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  • 42
    Keywords: Pharmaceutical technology ; Pharmacy ; Nanochemistry ; Pharmaceutical Sciences/Technology ; Pharmacy ; Nanochemistry ; Springer eBooks
    Description / Table of Contents: Understanding the Surface Characteristics of Nanoparticles -- Surface Modification of Nanoparticles for Targeted Drug Delivery -- Surface-Modified PLGA Nanoparticles for Targeted Drug Delivery to Neurons -- Nanoparticles for Site Specific Drug Delivery -- Stealth Properties of Nanoparticles against Cancer: Surface Modification of NPs for Passive Targeting to Human Cancer Tissue in Zebra Fish -- Passive targeting of nanoparticles to cancer -- Surface Modification of Magnetic Nanoparticles in Biomedicine -- A novel strategy for the surface modification of super paramagnetic (Fe3O4) iron oxide nanoparticle for lung cancer imaging -- Biodegradable nanoparticles for drug delivery and targeting -- Functionalized antibacterial nanoparticles in controlling biofilm and intracellular infections -- Surface modifications of liposomes for drug targeting -- Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system -- Surface Modification of Metallic Nano particles -- Successful Delivery of Zidovudine-loaded Docosanol Nanostructured Lipid Carriers (Docosanol NLCs) into rat brain -- Brain targeted drug delivery with surface modified nanoparticles -- Surface Modification of Resorcinarene Based Self-Assembled Solid Lipid Nanoparticles for Drug Targeting -- Targeted delivery of surface modified nanoparticles:Modulation of inflammation for acute lung injury -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface modification of Nano carriers for specific cell targeting for better therapeutic effect -- Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers -- Surface Modification of Gold Nanoparticles for Targeted Drug Delivery -- Surface modification and bioconjugation of nanoparticles for MRI technology -- Surface Modified Lanthanide Nanomaterials for Drug Delivery -- Engineering of targeted nanoparticles by using self-assembled biointegrated block copolymers -- Application of Nanoparticles in treating Periodontitis: Preclinical and Clinical overview -- Surface Modification of Nanoparticles for Ocular Drug Delivery
    Abstract: This unique book discusses various new techniques developed to enhance the application of nanoparticulate drug delivery systems using surface modification of nanoparticles. The understanding of the surface characteristics of nanoparticles is growing significantly with the advent of new analytical techniques. Polymer chemistry is contributing to the development of many new versatile polymers which have abilities to accommodate many different, very reactive chemical groups, and can be used as a diagnostic tool, for better targeting, for more effective therapeutic results as well as for reducing the toxic and side effects of the drugs. Surface modification of such polymeric nanoparticles has been found by many scientists to enhance the application of nano-particles and also allows the nanoparticles to carry specific drug molecule and disease /tumor specific antibodies which refine and improve drug delivery. Surface Modification of Nanoparticles for Targeted Drug Delivery is a collection of essential information on various applications of surface modification of nano-particles and their disease specific applications for therapeutic purposes
    Pages: XVIII, 499 p. 142 illus., 110 illus. in color. : online resource.
    ISBN: 9783030061159
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  • 43
    Keywords: Proteomics ; Biochemistry ; Proteomics ; Protein Science ; Springer eBooks
    Description / Table of Contents: Pre- and Post-Analytical Factors in Biomarker Discovery -- Pre-Fractionation of Non-Circulating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers -- Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers -- Protein Biomarker Discovery Using Human Blood Plasma Microparticles -- A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry -- Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery -- Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies -- Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics -- Protein Biomarker Discovery in Non-Depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry -- Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry -- Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid -- Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions -- Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues -- Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications -- Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR -- Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis using CombiROC -- PanelomiX for the Combination of Biomarkers -- Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework
    Abstract: This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 292 p. 52 illus., 47 illus. in color. : online resource.
    ISBN: 9781493991648
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  • 44
    Keywords: Genetics ; Evolution (Biology) ; Genetics and Genomics ; Evolutionary Biology ; Springer eBooks
    Description / Table of Contents: Setting Up an Ancient DNA Laboratory -- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate -- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step -- Extraction of Highly Degraded DNA from Ancient Bones and Teeth -- Sampling and Extraction of Ancient DNA from Sediments -- Extraction of Ancient DNA from Plant Remains -- DNA Extraction from Keratin and Chitin -- Double-Stranded Library Preparation for Ancient and Other Degraded Samples -- A Method for Single-Stranded Ancient DNA Library Preparation -- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms -- Whole-Genome Capture of Ancient DNA Using Homemade Baits -- Generating RNA Baits for Capture-Based Enrichment -- Hybridization Capture of Ancient DNA Using RNA Baits -- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA -- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis -- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA -- Authentication and Assessment of Contamination in Ancient DNA -- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence
    Abstract: This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab
    Pages: X, 216 p. 15 illus., 5 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991761
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  • 45
    Keywords: Bacteriology ; Enzymes ; Immunology ; Bacteriology ; Enzymology ; Immunology ; Springer eBooks
    Description / Table of Contents: 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus
    Abstract: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets
    Pages: XIII, 312 p. 70 illus., 41 illus. in color. : online resource.
    ISBN: 9781493991549
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  • 46
    Keywords: Biochemistry ; Cytology ; Protein Science ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Cloning and Mutagenesis Strategies for Large Collagens -- Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen -- Production and Characterization of Chemically Cross-Linked Collagen Scaffolds -- A Biomimetic Model for Mineralization of Type-I Collagen Fibrils -- Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy -- Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry -- Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes -- Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics -- Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy -- In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology -- Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics -- Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix -- Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans -- Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis -- Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment -- FACS Analysis of Col1α Protein Levels in Primary Fibroblasts -- Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors
    Abstract: This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study
    Pages: XI, 254 p. 60 illus., 44 illus. in color. : online resource.
    ISBN: 9781493990955
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  • 47
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: An Overview of CE in Clinical Analysis -- Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies -- Monitorization of α1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF Detection -- Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic Injection -- On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin -- Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis -- The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection -- Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization -- A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of Experiment -- New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS) -- Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Sample -- Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis -- Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis -- Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions -- A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood -- Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA) -- Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis -- Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen -- Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins
    Abstract: This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research
    Pages: XII, 273 p. 74 illus., 51 illus. in color. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493992133
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  • 48
    Keywords: Immunology ; Laboratory animals ; Immunology ; Animal Models ; Springer eBooks
    Description / Table of Contents: CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells -- Genome Editing in Mouse Embryos with CRISPR/Cas9 -- Derivation of Macrophages from Mouse Bone Marrow -- Bone Marrow Derived Dendritic Cells -- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils -- In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets -- Generation and Culture of Mouse Embryonic Fibroblasts -- Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation -- Depletion and Reconstitution of Macrophages in Mice -- Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making -- A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colz -- Mouse Model of Staphylococcus aureus Skin Infection -- Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses -- Endotoxin-Induced Uveitis in Rodents -- Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice -- Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages -- Influenza-Mediated Lung Infection Models -- Adoptive Transfer Colitis -- The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC) -- Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA) -- Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions -- Sepsis Induced by Cecal Ligation and Puncture
    Abstract: This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system
    Pages: XI, 258 p. : online resource.
    Edition: 2nd ed. 2019.
    ISBN: 9781493991679
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  • 49
    Keywords: Pharmaceutical technology ; Biomedical Engineering ; Pharmaceutical Sciences/Technology ; Biomedical Engineering/Biotechnology ; Springer eBooks
    Description / Table of Contents: Molecular Biotechnology -- Biophysical and biochemical analysis of recombinant proteins -- Production and downstream processing of biotech products -- Formulation of biotech products, including biopharmaceutical considerations -- Pharmacokinetics and pharmacodynamics of peptide and protein drugs -- Immunogenicity of pharmaceutical proteins -- General considerations of monoclonal antibodies from structure to therapeutic application -- Genomics, Other €œÓmicś€ Technologies, Personalized Medicine and Additional Biotechnology-Related Techniques -- Dispensing biotechnology products: handling, professional education and product information -- Economic considerations in medical biotechnology -- Regulatory framework for biosimilars -- Vaccines -- Oligonucleotides and siRNA -- Gene therapy -- Stem cell technology -- Therapeutic Applications.-Endocrinology -- Insulin -- Follicle-stimulating hormone -- Growth hormone -- Cardiovascular and Respiratory Applications -- Recombinant coagulation factors and thrombolytics -- Recombinant human deoxyribonuclease -- Oncology -- Monoclonal antibodies in oncology -- Hematopoetic growth factors -- Inflammation and Immunemodulation -- Monoclonal antibodies in transplantation -- Monoclonal antibodies and antibody-based therapeutics in anti-inflammatory therapy -- Interferons and interleukins -- Anti-infectious diseases -- Monoclonal antibodies -- Enzyme replacement therapy
    Abstract: This introductory text explains both the basic science and the applications of biotechnology derived pharmaceuticals, with special emphasis on their clinical use. It serves as a complete one-stop source for undergraduate/graduate pharmacists, pharmaceutical science students, and for those who did not receive formal training in this field. Pharmaceutical Biotechnology: Fundamentals and Applications, Fifth Edition completely updates the previous edition, and includes additional coverage on newer approaches such as oligonucleotides, mi/siRNA, gene therapy and advanced therapeutics. Daan J.A. Crommelin is Professor Emeritus Department of Pharmaceutical Sciences, Utrecht Institute for Pharmaceutical Sciences at Utrecht University, Utrecht, The Netherlands and Adjunct Professor in the Department of Pharmaceutics and Pharmaceutical Chemistry at the University of Utah, Salt Lake City, UT, USA Robert D. Sindelar is Professor and former Dean Faculty of Pharmaceutical Sciences and The Centre for Health Evaluation & Outcomes Sciences (CHEOS), The University of British Columbia (UBC), Vancouver, BC, Canada and President of the Global Drug Commercialization Centre (GDCC)-China and Vice-President GDCC-worldwide, Chengdu, China Bernd Meibohm is Professor of Pharmaceutical Sciences and Associate Dean for Research and Graduate Programs at the University of Tennessee Health Science Center, College of Pharmacy, Memphis, TN, USA
    Pages: XXIII, 653 p. 246 illus., 227 illus. in color. : online resource.
    Edition: 5th ed. 2019.
    ISBN: 9783030007102
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  • 50
    Keywords: Toxicology ; Biochemistry ; Oncology ; Pharmacology/Toxicology ; Biological and Medical Physics, Biophysics ; Biochemistry, general ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Part 1. Introduction -- Chapter 1. Antimicrobial peptides of multicellular organisms: My perspective -- Part 2. Mechanisms of Antimicrobial Action -- Chapter 2. Membrane permeabilization mechanisms -- Chapter 3. Elementary processes and mechanisms of interactions of antimicrobial peptides with membranes --- single GUV studies ---- -- Chapter 4. The mechanisms of action of cationic antimicrobial peptides refined by novel concepts from biophysical investigations -- Chapter 5. Anionic lipid clustering model -- Chapter 6. Intracellular antimicrobial peptides targeting the protein synthesis machinery -- Part 3. Other activities of AMPs -- Chapter 7. Antimicrobial and cell-penetrating peptides: How to understand two distinct functions despite similar physicochemical properties -- Chapter 8. Synthetic anti-lipopolysaccharide peptides (SALP) as effective inhibitors of pathogen-associated molecular patterns (PAMPs) -- Chapter 9. Anti-cancer activities of natural and synthetic peptides -- Chapter 10. Antimicrobial host defence peptides: Immunomodulatory functions and translational prospects -- Part 4. Towards Clinical Applications -- Chapter 11. Activity and selectivity of antimicrobial peptides: A complex interplay of multiple equilibria -- Chapter 12. Design of antimicrobial peptides: Progress made with human cathelicidin LL-37 -- Chapter 13. Application of synthetic molecular evolution to the discovery of antimicrobial peptides -- Chapter 14. AMPs as anti-biofilm agents for human therapy and prophylaxis -- Chapter 15. Clinical application of AMPs
    Abstract: This book presents an overview of antimicrobial peptides (AMPs), their mechanisms of antimicrobial action, other activities, and various problems that must still be overcome regarding their clinical application. Divided into four major parts, the book begins with a general overview of AMPs (Part I), and subsequently discusses the various mechanisms of antimicrobial action and methods for researching them (Part 2). It then addresses a range of activities other than antimicrobial action, such as cell penetration, antisepsis, anticancer, and immunomodulatory activities (Part 3), and explores the prospects of clinical application from various standpoints such as the selective toxicity, design, and discovery of AMPs (Part 4). A huge number of AMPs have been discovered in plants, insects, and vertebrates including humans, and constitute host defense systems against invading pathogenic microorganisms. Consequently, many attempts have been made to utilize AMPs as antibiotics. AMPs could help to solve the urgent problem of drug-resistant bacteria, and are also promising with regard to sepsis and cancer therapy. Gathering a wealth of information, this book will be a bible for all those seeking to develop antibiotics, anti-sepsis, or anticancer agents based on AMPs
    Pages: VIII, 304 p. 73 illus., 55 illus. in color. : online resource.
    ISBN: 9789811335884
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  • 51
    Keywords: Neurosciences ; Artificial intelligence ; Social sciences / Data processing ; Neurosciences ; Artificial intelligence ; Computer Appl. in Social and Behavioral Sciences ; Springer eBooks
    Description / Table of Contents: Preface -- Philosophy.-Blended cognition The robotic challenge -- Manipulative Abduction, External Semiotic Anchors, and Blended Cognition -- Practical Reasoning in the Deliberations of an Intelligent Autonomous Agent -- Blended Automation: The Language-Game of Psychoanalytic Automatism and Cybernetic Automata.-Engineering -- Can our Robots rely on an Emotionally Charged Vision-for- Action? An Embodied Model for Neurorobotics -- Modeling psycho-emotional states via neurosimulation of monoamine neuro-Transmitters -- Towards Conversation Envisioning for Cognitive Robots -- Psychology -- The blending of human and autonomous-machine cognition -- Heuristics of Numerical Choice in Economic Contexts -- Anthropology -- Towards autonomous artificial agents? Proposal for a naturalistic activity-based model of (artificial) life -- Logics -- Toward a Logic of Everyday Reasoning -- Index
    Abstract: This edited volume is about how unprejudiced approaches to real human cognition can improve the design of AI. It covers many aspects of human cognition and across 12 chapters the reader can explore multiple approaches about the complexities of human cognitive skills and reasoning, always guided by experts from different but complimentary academic fields. A central concept is explained: blended cognition, the natural skill of human beings for combining constantly different heuristics during their several task-solving activities. Something that was sometimes observed like a problem as “bad reasoning”, is now the central key for the understanding of the richness, adaptability and creativity of human cognition. The topic of this book connects in a significant way with the disciplines of psychology, neurology, anthropology, philosophy, logics, engineering, logics, and AI. In a nutshell: understanding better humans for designing better machines. Any person with interests on natural and artificial reasoning should read this book as a primary source of inspiration and a way to achieve a critical thinking on these topics
    Pages: X, 304 p. 54 illus., 24 illus. in color. : online resource.
    ISBN: 9783030031046