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  • DKFZ Publication Database  (1)
  • C-JUN  (1)
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  • DKFZ Publication Database  (1)
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  • Wiley  (1)
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    Keywords: TRANSPLANTATION ; C-JUN ; cell-cell interaction ; cell proliferation ; GM-CSF ; organotypic culture ; cell differentiation ; dermal fibroblasts ; epidermal keratinocytes ; epithelial cell culture ; dermal equivalent ; paracrine factors ; collagen matrix ; IL-1 ; KGF ; JunB
    Abstract: This chapter focuses on the role of dermal fibroblasts in epidermal keratinocyte proliferation and differentiation and proves the usefulness of an organotypic in vitro skin equivalent model to study mechanisms of tissue regeneration. Examples are given how these can be used to study the growth of keratinocytes after transplantation in vivo, alone or with a collagen matrix. This leads on to the development of in vitro organotypic models to generate skin equivalents composed of an organized epidermis on matrix-embedded dermal fibroblasts. Protocols are provided for both the in vivo transplantation models and the combined stromal/epithelial in vitro organotypic models, along with several variations of the methods. Characterization of the proliferation and differentiation of the cultures is described with an extensive discussion of recent results on the factors controlling tissue regeneration, with emphasis on the role of c-jun and junB gene products in the production of KGF and GM-CSF by fibroblasts in response to epithelium-derived IL1, and the effect of these cytokines on the proliferation and differentiation of epidermal keratinocytes.
    Type of Publication: Book chapter
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