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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 294 (1992), S. 466-478 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 317 (1993), S. 474-484 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    Unknown
    Unknown
    Philadelphia : Wolters Kluwer
    Type of Medium: Unknown
    Pages: vii, 668 p. : , ill.
    Edition: 7th ed.
    ISBN: 9781975112516
    Language: English
    Note: For online access to this volume please contact the library staff in room D124 (phone 3661, e-mail: http://www.dkfz.de/de/zbib/mitarbeiter/kontakt/fernleihe.php)
    Location/Call number: Library / QR360:271(7)/4
    Location/Call number: F035 / F035:014
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  • 4
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Bacteria. ; Cytology. ; Bacteria. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource.
    Type of Medium: Online Resource
    Pages: XI, 240 p. 39 illus., 31 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634912
    Series Statement: Methods in Molecular Biology, 2727
    Language: English
    Note: Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall -- Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography -- Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy -- Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi -- Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis -- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum -- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids -- Type I Lipoteichoic Acid (LTA) Detection by Western Blot -- Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy -- Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris -- Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography -- Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy -- Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase -- Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins -- Tracking Cell Wall Anchored Proteins in Gram-Positive Bacteria -- Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy -- Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers -- Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy.
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  • 5
    Subject(s): Bacteria. ; Microbiology. ; Bacteria. ; Microbiology.
    In: Springer Nature eBook
    Description / Table of Contents: This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
    Type of Medium: Online Resource
    Pages: X, 246 p. 51 illus., 40 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634738
    Series Statement: Methods in Molecular Biology, 2721
    Language: English
    Note: CRISPR/Cas9-based genome editing of Pseudomonas aeruginosa -- Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis using Mobile-CRISPRi -- Engineering green-light-responsive heterologous gene expression in Pseudomonas -- Fluorescence-based evaluation of cyclic di-GMP levels in Pseudomonas aeruginosa -- Whole-cell biosensors for qualitative and quantitative analysis of quorum sensing signal molecules and the investigation of quorum quenching agents -- A Pseudomonas aeruginosa-suitable fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs -- The Pseudomonas aeruginosa resistome: permanent and transient antibiotic resistance, an overview -- Biosensors for inducers of transient antibiotic resistance -- Pseudomonas aeruginosa soluble pyocins as antibacterial weapons -- Assays for studying Pseudomonas aeruginosa secreted proteases -- Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms -- Broad genome sequencing of environmental and clinical strains and genotyping -- Genome-scale analysis of the structure and function of RNA pathways and networks in Pseudomonas aeruginosa -- In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. Improving the predictive value of preclinical mouse models of Pseudomonas aeruginosa respiratory infection to evaluate antibiotic efficacy -- Emerging in vitro models for the study of infection and pathogenesis of Pseudomonas aeruginosa and testing of antibacterial agents.
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  • 6
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Immunology. ; Allergy. ; Food science. ; Immunology. ; Allergology. ; Food Science.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Food Allergens: Methods and Protocols serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components.
    Type of Medium: Online Resource
    Pages: XII, 377 p. 73 illus., 57 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634530
    Series Statement: Methods in Molecular Biology, 2717
    Language: English
    Note: Food Allergens of Plant and Animal Origin: Classification, Characteristics, and Properties -- Purification of Food Allergens from Their Natural Sources: Chromatographic Methods -- Recombinant Production of Food Allergens in Yeast Pichia pastoris -- Yeast Surface Display Methodology for the Characterization of Food Allergens In Situ -- Characterization of Linear IgE-Binding Epitopes in Food Allergens -- Characterization of T-Cell Epitopes in Food Allergens by Bioinformatic Tools -- Phage Immunoprecipitation Sequencing (PhIP-Seq) for Analyzing Antibody Epitope Repertoires against Food Antigens -- 1D-, 2D-Gel Electrophoresis, Immunoblotting, and Enzyme-Linked Immunosorbent Assay (ELISA) for the Study of Food Allergens -- Preparation of Blinded Food Matrixes for Clinical Oral Challenges -- Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy -- Co-Culture of Human Dendritic and T Cells for the Study of Specific T Cell-Mediated Responses against Food Allergens -- Evaluation of the Suppressive Capacity of Regulatory T Cells in Food Allergy Research -- Mass Cytometry in Food Allergy Research -- Indirect Basophil Activation Test for Peanut Allergy Diagnosis Using Human Donor Basophils -- Standardization of Food Allergen Measurements Using Multiplex Array Technology -- Biosensors for the Detection of Food Allergens -- Standardization of a Mass Spectrometry-Based Workflow for Food Allergen Quantification -- Identifying Similar Allergens and Potentially Cross-Reacting Areas Using Structural Database of Allergenic Proteins (SDAP) Tools and D-Graph -- Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA) -- Detection of Bet v 1 Homologous Proteins and Plant Profilins by Indirect ELISA -- A Mouse Model of Shrimp Allergy with Cross-Reactivity to Crab and Lobster -- Animal Models in the Study of Food Allergens: Long-Term Maintenance of Allergic Reactivity in Mouse Models of Food Allergy -- Study of MicroRNAs Expression in Food Allergy -- De Novo Transcriptomic Analyses to Identify and Compare Allergens in Foods -- Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research.
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  • 7
    Subject(s): Proteins. ; Therapeutics. ; Biomedical engineering. ; Proteins. ; Therapeutics. ; Biomedical Engineering and Bioengineering.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins. .
    Type of Medium: Online Resource
    Pages: XI, 248 p. 62 illus., 47 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634691
    Series Statement: Methods in Molecular Biology, 2720
    Language: English
    Note: Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production -- Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains -- Selective and Site-Specific Incorporation of Non-Standard Amino Acids within Proteins for Therapeutic Applications -- An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions -- Split-Protein Therapeutic Platforms: Identifying Binder Pairs -- Metal-Mediated Ligand Affinity Chemistry (MLAC) -- Recombinant Elastin‐Based Bioelastomers for Biomedical Applications -- Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery -- Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines -- Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs -- Unnatrual Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics -- Formulation of Chitosan-Zein Nano-in-Microparticles for Oral DNA Delivery -- Engineering E2 Bionanoparticles for Targeted Delivery of Chemotherapeutics to Breast Cancer Cells -- Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes -- Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines -- Melt-Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants.
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  • 8
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Biomedical engineering. ; Genetics. ; Bioinformatics. ; Biomedical Engineering and Bioengineering. ; Genetics. ; Bioinformatics.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters guide readers through culprit genes, in-silico drug discovery techniques, genome-wide ChIP-X data, high-Throughput Transcriptomic Data Exome Sequencing, Next-Generation Sequencing, Fuorescence Spectroscopy, data analysis in Bioinformatics, Computational Biology, and S-system based modeling of GRN. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Engineering of Regulatory Networks aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
    Type of Medium: Online Resource
    Pages: X, 327 p. 72 illus., 64 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634615
    Series Statement: Methods in Molecular Biology, 2719
    Language: English
    Note: Molecular Modeling Techniques and in-Silico Drug Discovery -- Systems Biology Approach to Analyse Microarray Datasets for Identification of Disease-Causing Genes: Case Study of Oral Squamous cell Carcinoma -- Fluorescence Spectroscopy: A Useful Method to Explore the Interactions of Small Molecule Ligands with DNA Structures -- Inference of Dynamic Growth Regulatory Network in Cancer Using high-Throughput Transcriptomic Data -- Implementation of Exome Sequencing to Identify Rare Genetic Diseases -- Emerging Trends in Big Data Analysis in Computational Biology and Bioinformatics in Health Informatics: A Case Study on Epilepsy and Seizures -- New Insights into Clinical Management for Sickle-Cell Disease: Uncovering the Significance Pathways Affected By the Involvement of Sickle Cell Disease -- A Review on Computational Approach for S-system Based Modeling of Gene Regulatory Network -- Big Data in Bioinformatics and Computational Biology: Basic Insights -- Identification of Culprit Genes for Different Diseases by Analysing Microarray Data -- Big Data Analysis in Computational Biology and Bioinformatics -- Prediction and Analysis of Transcription Factor Binding Sites to Understand Gene Regulation: Practical Examples and Case Studies using R Programming -- Hubs and Bottlenecks in Protein-Protein Interaction Networks -- Next-Generation Sequencing to Study the DNA Interaction Nac Deep Learning for Predicting Gene Regulatory Networks: A Step-by-Step Protocol in R -- Deep Learning for Predicting Gene Regulatory Networks: A Step-by-Step Protocol in R -- Computational inference of Gene Regulatory Network using genome-wide ChIP-X data -- Reverse Engineering in Biotechnology: The Role of Genetic Engineering in Synthetic Biology.
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  • 9
    Subject(s): Biophysics. ; Biotechnology. ; Biophysics. ; Biotechnology.
    In: Springer Nature eBook
    Description / Table of Contents: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. .
    Type of Medium: Online Resource
    Pages: XIV, 511 p. 130 illus., 109 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071633779
    Series Statement: Methods in Molecular Biology, 2694
    Language: English
    Note: Introduction to Optical Tweezers: Background, System Designs, and Applications -- Quantifying ATP-Independent Nucleosome Chaperone Activity with Single Molecule Methods -- Protein Tethering for Single-Molecule Force Spectroscopy -- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies -- Probing Mitotic Chromosome Mechanics Using Optical Tweezers -- A Brief Introduction to Single-Molecule Fluorescence Methods -- Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans -- Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays -- Fluorescence Microscopy of Nanochannel-Confined DNA -- Single-Molecule FRET X -- Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Sites of Nucleoprotein Complexes -- Measuring Transcription Dynamics of Individual Genes Inside Living Cells -- Single-Molecule FRET Resolved Protein Dynamics from Plasmid to Data in Six Steps -- Atomic Force Microscopy: An Introduction -- Atomic Force Microscopy of Viruses: Stability, Disassembly, and Genome Release -- Unfolding and Refolding Proteins using Single-Molecule AFM -- Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy -- An Introduction to Magnetic Tweezers -- Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay -- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions -- Detecting DNA Loops Using Tethered Particle Motion -- Single-Cell Measurements using Acoustic Force Spectroscopy (AFS) -- DNA Origami-Based Single-Molecule Force Spectroscopy and Applications.
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  • 10
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cell organelles. ; Cytology. ; Organelles. ; Cell Biology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. .
    Type of Medium: Online Resource
    Pages: XII, 278 p. 56 illus., 50 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071635070
    Series Statement: Methods in Molecular Biology, 2725
    Language: English
    Note: Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium -- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures -- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells -- Expansion Microscopy of Ciliary Proteins -- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes -- Structural Analysis of Sperm Centrioles Using N-STORM -- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium -- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets -- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging -- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells -- Proximity Mapping of Ciliary Proteins by BioID -- Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags -- Primary Human Nasal Epithelial Cell Culture -- BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation -- High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models -- Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods -- Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.
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  • 11
    Unknown
    Unknown
    Weinheim : Wiley-VCH
    Type of Medium: Unknown
    Pages: 206 p. : , ill
    ISBN: 9783527721375
    Series Statement: Lernen leicht gemacht
    Language: English
    Note: privat : verantwortungsvoller Umgang mit ChatGPT und anderen KI-Tools Anmerkung: Titelzusatz auf Cover: Technische Grundlagen von ChatGPT und OpenAI. ChatGPT gewinnbringend nutzen: beim Lernen, bei der Arbeit und privat. Verantwortungsvoller Umgang mit ChatGPT und anderen KI-Tools.
    Location/Call number: Library / ordered
    Location/Call number: M120 / M120:413
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  • 12
    Unknown
    Unknown
    Heymanns
    Type of Medium: Unknown
    Edition: 12. Aufl.
    ISBN: 9783452302083
    Location/Call number: T010 / ordered
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  • 13
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Genetic transcription. ; Biology Technique. ; Gene expression. ; Gene Transcription. ; Gene Expression Analysis.
    In: Springer Nature eBook
    Description / Table of Contents: This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deadenylation: Methods and Protocols aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression.
    Type of Medium: Online Resource
    Pages: XI, 326 p. 58 illus., 47 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634813
    Series Statement: Methods in Molecular Biology, 2723
    Language: English
    Note: The reconstitution of human CCR4-NOT from purified proteins and an assay of its deadenylation activity -- In vitro reconstitution of the Drosophila melanogaster CCR4-NOT complex to assay deadenylation -- Analysis of human mRNA deadenylation complexes via high-resolution gel electrophoresis -- Quantitative biochemical analysis of deadenylase enzymes using fluorescence and chemiluminescence-based assays -- A FRET-based assay to quantify enzymatic rates and explore the mechanisms of RNA deadenylases in heterogeneous environments -- Measuring Poly-Adenosine Tail Length of RNAs by High Resolution Northern Blotting Coupled with RNase H Cleavage -- An RNA-ligation-based RACE-PAT assay to monitor poly(A) tail length of mRNAs of interest -- Dissecting the role of the Ccr4-Not deadenylase complex in pluripotency and differentiation -- Measuring proximity-mediated function of mRNA regulatory proteins by engineered tethering.-Tethered mRNA Amplifier: A Novel Approach to Increase Protein Expression -- RNA binding protein-mediated mRNA deadenylation in mammalian cell extracts -- Transcriptome-wide analysis of mRNA adenylation status in yeast using nanopore sequencing -- Sequencing of transcriptome-wide poly(A) tails by PAIso-seq -- Nano3′RACE: a method to analyze poly(A) tail length and nucleotide additions at the 3′ extremity of selected mRNAs using nanopore sequencing -- Quantification of poly(A) tail length and terminal modifications using direct RNA sequencing -- Differential poly(A) tail length analysis using nanopore sequencing -- Single-molecule poly(A) tail sequencing (SM-PATseq) using the PacBio platform -- Mathematical models for the poly(A) tail shortening process.
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  • 14
    Online Resource
    Online Resource
    Cham : Springer International Publishing
    Subject(s): Food Microbiology. ; Food science. ; Food Sensory evaluation. ; Nutrition   . ; Food Analysis. ; Chemistry. ; Food Microbiology. ; Food Science. ; Sensory Evaluation. ; Nutrition. ; Food Chemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This Open Access book covers the concept of umami, the unique taste imparted by the amino acid glutamate, was first described in 1908 by Dr. Kikunae Ikeda of Tokyo University. Over the past century, hundreds of studies have explored the mechanistic underpinnings of the taste, leading to the characterization of the umami taste receptor in 2002. How this fifth basic taste figures into nutrition and health, however, remains underexplored. Umami: Taste for Health provides an overview of the relationship between umami and human health. Authors explain how glutamate not only produces a characteristic oral sensation in the mouth but also functions as a signaling molecule to induce physiological responses. With the support of recent studies, the book demonstrates how the taste properties of umami make glutamate a promising substance to lower salt intake, promote satiation and support healthier aging. The text also covers practical culinary applications to increase umami flavor and practical usage of umami for promoting healthy eating. Provides an overview of the relationship between umami and human health; Explores the potential of glutamate to lower salt intake, promote satiation and support healthier aging; Covers practical culinary applications of umami flavor and practical usage of umami for promoting healthy eating.
    Type of Medium: Online Resource
    Pages: XIII, 198 p. 33 illus. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9783031326929
    Series Statement: Food and Health,
    Language: English
    Note: 1. Umami and MSG -- 2. Sensory Physiology of Umami -- 3. Umami and Salty: A Cooperative Pair -- 4. Protein, Umami and Satiety -- 5. Development and Umami -- 6. Umami and Healthy Aging -- 7. Umami As A Component of Healthy Diets -- 8. Practicalities from Culinology.
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  • 15
    Subject(s): Biological models. ; Biology Technique. ; Biological Models. ; Biological Techniques.
    In: Springer Nature eBook
    Description / Table of Contents: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
    Type of Medium: Online Resource
    Pages: XIV, 360 p. 94 illus., 85 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071634011
    Series Statement: Methods in Molecular Biology, 2707
    Language: English
    Note: Delivering Traumatic Brain Injury to Larval Zebrafish -- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish -- Methods to Study Liver Disease Using Zebrafish Larvae -- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening -- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ) -- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions -- Primary Culture of Neuronal Populations for Various Downstream Applications -- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish -- Brain Imaging and Registration in Larval Zebrafish -- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy -- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish -- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows -- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette -- Spinal Cord Injury and Assays for Regeneration -- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase -- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.
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  • 16
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Pharmacology. ; Chemistry Data processing. ; Bioinformatics. ; Pharmacology. ; Computational Chemistry. ; Bioinformatics.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition provides new and updated methods and techniques for identification of drug target, binding sites prediction, high- throughput virtual screening, lead discovery and optimization, conformational sampling, prediction of pharmacokinetic properties using computer-based methodologies. Chapters also focus on the application of the latest artificial intelligence technologies for computer aided drug discovery. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computational Drug Discovery and Design, Second Edition aims to effectively utilize computational methodologies in discovery and design of novel drugs.
    Type of Medium: Online Resource
    Pages: XI, 356 p. 1 illus. , online resource.
    Edition: 2nd ed. 2024.
    ISBN: 9781071634417
    Series Statement: Methods in Molecular Biology, 2714
    Language: English
    Note: Computer-Aided Drug Discovery and Design – Recent Advances and Future Prospects -- Virtual Screening Process - A Guide in Modern Drug Designing -- Molecular dynamics as a tool for virtual ligand screening -- Antiviral Drug Target Identification and Ligand Discovery -- GRAMM webserver for protein docking -- Protein–ligand blind docking using CB-Dock2 -- Applications of Molecular Dynamics Simulations in Drug Discovery -- Molecular dynamics simulation-based prediction of glycosaminoglycan interactions with drug molecules -- Mining chemogenomic spaces for prediction of drug-target interactions -- Expanding the landscape of amyloid sequences with CARs-DB: a database of polar amyloidogenic peptides from disordered proteins -- Accelerating molecular dynamics simulations for drug discovery -- Exploring the Role of Chemoinformatics in Accelerating Drug Discovery: A Computational Approach. -Recent Deep-Learning Applications to Structure-Based Drug Design -- Techniques for Developing Reliable Machine Learning Classifiers Applied to Understanding and Predicting Protein:Protein Interaction Hot Spots -- AI driven enhancements in drug screening and optimisation -- Applications of big data and AI-driven technologies in CADD (computer-aided drug design) -- Artificial Intelligence in ADME Property Prediction -- Accelerating the discovery and design of antimicrobial peptides with artificial intelligence.
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  • 17
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurosciences. ; Neuroscience.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the latest research and development in the areas of Vagus Nerve Stimulation (VNS) as it relates to bioelectronic medicine from neonate to adult. The chapters in this book cover topics such as invasive and non-invasive VNS including methodological considerations (study design, stimulation parameters, and use of heart rate variability metrics); mechanisms of action (automatic regulation and immune plasticity); and disorders where VNS approaches may be therapeutic (migraine and cluster headaches, mood disorders, trauma-related disorders, and language learning). In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Vagus Nerve Stimulation is a valuable resource for both novice and expert preclinical and clinical scientists, clinicians, physicians, and scholars who are interested in learning more about this exciting and developing field.
    Type of Medium: Online Resource
    Pages: XXII, 206 p. 26 illus., 19 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634653
    Series Statement: Neuromethods, 205
    Language: English
    Note: Vagus Nerve Stimulation in Peripheral Targets -- Vagal Nerve Stimulation Through the Lens of the Polyvagal Theory: Recruiting Neurophysiological Mechanisms to Dampen Threat Reactions and Promote Homeostatic Functions -- Heart Rate Variability as a Biomarker for Electrical Vagus Nerve Stimulation -- Vagus Nerve Manipulation and Microglial Plasticity in the Prenatal Brain -- Neonatal Sepsis is Diminished by Cervical Vagus Nerve Stimulation and Tracked Non-Invasively by ECG: A Pilot Report and Dataset in the Piglet Model -- Cognitive Enhancement through Vagus Nerve Stimulation: Methodological Considerations for Behavioral Studies in Rats -- Better Mood through Vagus Nerve Stimulation -- Transcutaneous Vagal Nerve Stimulation in Trauma Spectrum Psychiatric Disorders -- Vagus Nerve Stimulation for Migraine and Cluster Headaches -- Vagus Nerve Stimulation and Language Learning. .
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  • 18
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Bioinformatics. ; Pharmacology. ; Medicine Research. ; Biology Research. ; Computational and Systems Biology. ; Pharmacology. ; Biomedical Research.
    In: Springer Nature eBook
    Description / Table of Contents: This volume explores the application of high-performance computing (HPC) technologies to computational drug discovery (CDD) and biomedicine. The first section collects CDD approaches that, together with HPC, can revolutionize and automate drug discovery process, such as knowledge graphs, natural language processing (NLP), Bayesian optimization, automated virtual screening platforms, alchemical free energy workflows, fragment-molecular orbitals (FMO), HPC-adapted molecular dynamic simulation (MD-HPC), and the potential of cloud computing for drug discovery. The second section delves into computational algorithms and workflows for biomedicine, featuring an HPC framework to assess drug-induced arrhythmic risk, digital patient applications relevant to the clinic, virtual human simulations, cellular and whole-body blood flow modeling for stroke treatments, prediction of the femoral bone strength from CT data, and many more subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary software and tools, step-by-step and readily reproducible modeling protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Performance Computing for Drug Discovery and Biomedicine allows a diverse audience, including computer scientists, computational and medicinal chemists, biologists, clinicians, pharmacologists and drug designers, to navigate the complex landscape of what is currently possible and to understand the challenges and future directions of HPC-based technologies.
    Type of Medium: Online Resource
    Pages: XIII, 429 p. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634493
    Series Statement: Methods in Molecular Biology, 2716
    Language: English
    Note: Introduction to Computational Biomedicine -- Introduction to High Performance Computing -- Computational Biomedicine (CompBioMed) Centre of Excellence: Selected Key Achievements -- In Silico Clinical Trials: Is It Possible? -- Bayesian Optimization in Drug Discovery -- Automated Virtual Screening -- The Future of Drug Development with Quantum Computing -- Edge, Fog, and Cloud Against Disease: The Potential of High-Performance Cloud Computing for Pharma Drug Discovery -- Knowledge Graphs and Their Applications in Drug Discovery -- Natural Language Processing for Drug Discovery Knowledge Graphs: Promises and Pitfalls -- Alchemical Free Energy Workflows for the Computation of Protein-Ligand Binding Affinities -- Molecular Dynamics and Other HPC Simulations for Drug Discovery -- High Throughput Structure-Based Drug Design (HT-SBDD) Using Drug Docking, Fragment Molecular Orbital Calculations, and Molecular Dynamic Techniques -- HPC Framework for Performing In Silico Trials Using a 3D Virtual Human Cardiac Population as Means to Assess Drug-Induced Arrhythmic Risk -- Effect of Muscle Forces on Femur during Level Walking Using a Virtual Population of Older Women -- Cellular Blood Flow Modeling with HemoCell -- A Blood Flow Modeling Framework for Stroke Treatments -- Efficient and Reliable Data Management for Biomedical Applications -- Accelerating COVID-19 Drug Discovery with High-Performance Computing -- Teaching Medical Students to Use Supercomputers: A Personal Reflection.
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  • 19
    Subject(s): Immunity. ; Hypersensitivity ; Immune System physiology ; Immunologic Deficiency Syndromes.
    Type of Medium: Book
    Pages: v, 345 pages : , illustrations (chiefly color) ; , 24 cm
    Edition: 7th edition.
    ISBN: 9780443105197
    Language: English
    Location/Call number: Library / QR181:099(7)
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  • 20
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cytology. ; Immunology. ; Bacteria. ; Cell Biology. ; Immunology. ; Bacteria.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field.
    Type of Medium: Online Resource
    Pages: XVI, 576 p. 128 illus., 120 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634370
    Series Statement: Methods in Molecular Biology, 2713
    Language: English
    Note: Macrophage Development and Function -- Fate Mapping Macrophages: From Ontogeny to Functions -- Studying Autophagy in Microglia: Overcoming the Obstacles -- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq -- Isolation of Tissue Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages -- Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue -- Isolation and Flow Cytometry Analysis of Macrophages from the Dermis -- Isolation and Flow Cytometry Analysis of Macrophages from the Kidney -- Isolation and Flow Cytometry Analysis of Intestinal Macrophages -- Isolation and Characterization of Testis Macrophages Using Flow Cytometry -- Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy -- Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages -- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach -- Spectral Flow Cytometry Analysis of Resident Tissue Macrophages -- Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging -- 3D Imaging of Macrophages in Complete Organs -- Whole-Mount Imaging of Adipose Tissue Macrophages -- Functional In Vivo Imaging of Macrophages -- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy -- Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection -- Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH -- Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA) -- Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation -- Detection of G-Quadruplex DNA Structures in Macrophages -- Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models -- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis -- Automated Cell Counting of Macrophages In Situ -- Morphometric Analyses of Macrophages -- Combined Analysis of mRNA Expression and Open Chromatin in Microglia.
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  • 21
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Food science. ; Microbiology. ; Food Science. ; Microbiology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details preparations, separations, identification, analysis of postbiotics types and mechanism, uses of postbiotics in health, pharma, aquacultures, and the food industry. Divided into five sections chapters provide methods on antimicrobials, antibiofilm, anti-inflammatory, antiallergies, antiobesity, meurotransmitter activity, dietary supplementation and Immunomodulatory activity of postbiotics, postbiotics as biopreservatives , and as well as food packing material. . Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Postbiotics aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
    Type of Medium: Online Resource
    Pages: XXI, 424 p. 68 illus., 50 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634219
    Series Statement: Methods and Protocols in Food Science,
    Language: English
    Note: Isolation and Identification of Probiotics Microorganisms -- Isolation and Identification of Lactic Acid Bacteria -- Isolation and Identification of Bifidobacterium Spp. -- Isolation and Identification Of Yeasts -- Mass Propagation of Lactic Acid Bacteria -- Preparation of Postbiotics From Bifidobacterium Sp. -- Preparation of Postbiotics From Lactococcus Spp. -- Preparation of Postbiotics From Saccharomyces Spp. -- Preparation of Postbiotics From Streptomyces Sp. -- Preparation of Postbiotics From Streptococcus Sp. -- Preparation of Postbiotics from Bacillus -- Chemical Characterization and Identification of Postbiotics From Probiotic Microbes -- Thermal Methods of Postbiotics Preparation -- Non-Thermal Preparation of Postbiotics -- Next-Generation Sequence Analysis of Postbiotics In Fermented Dairy Food -- Next Generation Sequence Analysis of Postbiotics In Fermented non- Dairy Food -- Harvesting of Postbiotics And Its Assessment -- Analysis and Spectral Characterization of Exopolysaccharide Postbiotics by FT-IR and NMR -- Analysis and Identification Short Chain Fatty Acid Postbiotics By Gas Chromatography -- Analysis and identification of Biosurfactants Postbiotics -- Analysis and Identification Peptide Postbiotics By LC-MS, MOLTI-TOF Mass Spectrometry -- Analysis and Identification of Postbiotic Enzymes -- UHPLC And MS/MS Mediated Analysis And Quantification Of Postbiotic Cobalamin (Vitamin B12) From Propionibacterium Freudenreichii -- Separation and Identification Of Neurotransmitters From Of Postbiotics -- Immuno Modulatory Activity of Postbiotics From Lactobacillus -- Antibacterial Activity of Postbiotics -- Antifungal Activity of Postbiotics -- Antiviral Activity of Postbiotics -- Antiprotozoan Activity Of Postbiotics -- Anthelminthic Activity Of Postbiotics -- Antibiofilm Activity Of Postbiotics -- Evaluation Of Antioxidant Activity Of Postbiotics In Cell Cultures -- Anti-Allergies Activity Of Postbiotics -- Anti-inflammatory Activity of Postbiotics -- Anti-obesity activity of postbiotics -- Angiotensin 1-converting enzyme inhibitory postbiotics from fermented soybean -- Bacteriocin Postbiotics for tuberculosis drug development -- Postbiotics For Typhoid Drug Development -- Immunomodulatory Activity Of Postbiotics In Goat -- Immunomodulatory Activity of Postbiotics in Chicks -- Immunomodulatory Activity Of Postbiotics In Pigs -- Invivo Immunomodulatory Study of Postbiotics in Mice -- Dietary Supplementation and Immunomodulatory Activity Of Postbiotics In Fish -- Dietary Supplementation and Immunomodulatory Activity of Postbiotics in Shrimp -- Dietary Supplementation and Immunomodulatory activity of Postbiotics in Oyster -- Dietary supplementation and Immunomodulatory activity of Crab -- Bio preservation of meat and fish products using Postbiotics -- Biopreservation of Dairy Products through Postbiotics -- Biopresrvation Of Fruits And Vegetables Using Postbiotics -- Biopreservation of food using bacteriocin -- Postbiotics Food Packing Based on Organic acids -- Postbiotics Food Packaging using Peptides -- Postbiotics Food Packing using Bacteriocin -- Degradation of Chlorpyrifos pesticide using a postbiotic enzyme of Lactic acid bacteria -- Dosa Postbiotics Food Preparations -- Preservation of Postbiotics by Spray-Drying Microencapsulation .
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  • 22
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Medicine Research. ; Biology Research. ; Internal medicine. ; Biomedical Research. ; Internal Medicine.
    In: Springer Nature eBook
    Description / Table of Contents: This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs. .
    Type of Medium: Online Resource
    Pages: X, 264 p. 69 illus., 54 illus. in color. , online resource.
    Edition: 1st ed. 2024.
    ISBN: 9781071634295
    Series Statement: Methods in Molecular Biology, 2711
    Language: English
    Note: Determination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro -- Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice -- Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux -- Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy -- Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System -- Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model -- Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier -- Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy -- Studying Angiogenesis using Matrigel In Vitro and In Vivo -- Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy -- Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells -- Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs) -- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification -- Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay -- Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model -- Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells -- An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies -- Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro -- Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression -- Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer -- Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy.
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  • 23
    Subject(s): Neoplasms classification
    Type of Medium: Book
    Pages: xi, 425 p. : , ill.
    Edition: 7. Aufl.
    ISBN: 9783527350162
    Uniform Title: TNM atlas.
    Language: German
    Note: Translation of: TNM atlas - illustrated guide to the TNM classification of malignant tumours (2021).
    Location/Call number: Library / QZ200:016(7)
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  • 24
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS German Medical Science; VOL: 21; DOC01 /20230303/
    Publication Date: 2023-03-27
    Description: For the purposes of this guideline, a diving accident is defined as an event that is either potentially life-threatening or hazardous to health as a result of a reduction in ambient pressure while diving or in other hyperbaric atmospheres with and without diving equipment. This national consensus-based guideline (development grade S2k) presents the current state of knowledge and recommendations on the diagnosis and treatment of diving accident victims. The treatment of a breath-hold diver as well as children and adolescents does not differ in principle.In this regard only unusual tiredness and itching without visible skin changes are mild symptoms.The key action statements: on-site 100% oxygen first aid treatment, immobilization/no unnecessary movement, fluid administration and telephone consultation with a diving medicine specialist are recommended.Hyperbaric oxygen therapy (HBOT) remains unchanged as the established treatment in severe cases, as there are no therapeutic alternatives. The basic treatment scheme recommended for diving accidents is hyperbaric oxygenation at 280 kPa.
    Description: Ein Tauchunfall im Sinne dieser Leitlinie ist ein potenziell lebensbedrohliches oder gesundheitsschädigendes Ereignis, hervorgerufen durch Abfall des Umgebungsdruckes beim Tauchen oder aus sonstiger hyperbarer Atmosphäre mit und ohne Tauchgerät. Diese nationale S2k-Leitlinie legt den aktuellen Stand der Erkenntnisse und der konsentierten Empfehlungen in der Diagnostik und Behandlung von Patienten nach Tauchunfällen dar. Die Behandlung von Apnoetauchern sowie Kindern und Jugendlichen unterscheidet sich prinzipiell nicht.Milde Symptome sind nur die auffällige Müdigkeit und ein Hautjucken ohne sichtbare Hautveränderungen.Wesentliche Bedeutung bei der Versorgung von Tauchunfällen hat die frühzeitige Atmung von 100%igem Sauerstoff. Weiterhin werden die Ruhiglagerung/keine unnötige Bewegung, eine moderate Flüssigkeitsgabe und eine Taucherärztliche Telefonberatung empfohlen.Die hyperbare Sauerstofftherapie (HBOT) ist bei schweren Dekompressionsunfällen unverändert ohne therapeutische Alternative. Als Behandlungsschema wird grundsätzlich eine HBOT bei 280 kPa empfohlen.
    Subject(s): diving accident ; decompression sickness ; decompression illness ; arterial gas embolism ; oxygen ; hyperbaric oxygen therapy ; Tauchunfall ; Dekompressionserkrankung ; arterielle Gasembolie ; Sauerstoff ; hyperbare Sauerstofftherapie ; ddc: 610
    Language: English
    Type: article
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  • 25
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC07 /20230327/
    Publication Date: 2023-03-27
    Description: The objective is to provide a comprehensive overview of the rapidly developing field of the current state of research on in vivo use of hypochlorous acid (HOCl) to aid infection prevention and control, including naso-pharyngeal, alveolar, topical, and systemic HOCl applications. Also, examples are provided of dedicated applications in COVID-19. A brief background of HOCl's biological and chemical specifics and its physiological role in the innate immune system is provided to understand the effect of in vivo applications in the context of the body's own physiological defense mechanisms.
    Description: Es wird ein umfassender Überblick über den aktuellen Stand der Forschung zum In-vivo-Einsatz von hypochloriger Säure (HOCl) zur Infektionsprävention und -bekämpfung gegeben. Der Schwerpunkt liegt auf nasalen, alveolären und topischen Anwendungen. Außerdem werden Beispiele für spezielle Anwendungen bei COVID-19 vorgestellt. Um die Wirkung von HOCl im Zusammenwirken mit körpereigenen physiologischen Abwehrmechanismen zu verstehen, werden die biologischen und chemischen Besonderheiten von HOCl und seiner physiologischen Rolle im humanen Immunsystem erläutert
    Subject(s): hypochlorous acid ; in vivo application ; HOCl chemistry ; innate immune response ; tissue compatibility ; infection control ; inhalation ; wound care ; disinfection ; Hypochlorige Säure ; In-vivo-Anwendung ; HOCl-Chemie ; physiologische Immunantwort ; Gewebeverträglichkei ; Infektionsprävention ; Inhalation ; Wundpflege ; Desinfektion ; ddc: 610
    Language: English
    Type: article
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  • 26
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC03 /20230130/
    Publication Date: 2023-02-04
    Description: Purpose: To report an occult intraocular foreign body mimicking choroidal melanoma. Methods: Medical records and imagings of the patient were retrospectively reviewed.Case description: A 76-year-old male was referred to our ocular oncology clinic with a suspicious hyperpigmented retinal lesion in the left eye. Biomicroscopy showed aphakia and peripheral iridectomy in the left eye. Fundoscopy revealed a pigmented, slightly elevated lesion on the macula of the left eye surrounded by diffuse atrophy. B-scan ultrasonography showed a preretinal hyperechoic lesion with posterior shadowing. There was no choroidal mass in B-scan or optical coherence tomography (OCT) imaging. On further questioning, it was disclosed that the patient had been hit by an iron fragment in the left eye forty years ago.Conclusion: Choroidal melanoma is a vision- and life-threatening intraocular malignant tumour. Various neoplastic, degenerative, and inflammatory conditions can simulate choroidal melanoma. A previous history of penetrating ocular trauma should lead the surgeon to re-evaluate a diagnosis of melanoma.
    Subject(s): choroid hemorrhage ; choroid neoplasms ; choroidal neovascularization ; foreign bodies ; eye injuries ; Aderhautblutung ; Aderhautneoplasmen ; choroidale Neovaskularisation ; Fremdkörper ; Augenverletzungen ; ddc: 610
    Language: English
    Type: article
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  • 27
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    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC01 /20230117/
    Publication Date: 2023-01-18
    Description: Objective: The aim of this study was to determine the acceptance of Covid-19 vaccine among the Turkish adult population. Methods: A total of 2023 persons participated in this cross-sectional study between October 2020 and January 2021. The questionnaire, which was delivered via social media, was filled out by the participants over "Google Forms".Results: Questionnaire results showed that 68.7% of the participants might agree to vaccinated against COVID-19. According to univariate analysis, the age group of 50-59, urban residents, healthcare workers, non-smokers, and those with chronic diseases, those who were vaccinated against influenza, pneumonia, and tetanus were all willing to be vaccinated against COVID-19.Conclusions: It is very important to determine a community's willingness to be vaccinated against COVID-19 so that interventions can be made to solve related problems. Risk of exposure and importance of Prevention play a critical role in vaccination acceptance.
    Description: Zielsetzung: Ziel der Studie war es, die Akzeptanz der Covid-19 Impfung in der türkischen Erwachsenenbevölkerung zu ermitteln.Methode: An der Querschnittsstudie nahmen zwischen Oktober 2020 und Januar 2021 2023 Personen teil. Der Fragebogen, der über soziale Medien übermittelt wurde, wurde von den Teilnehmern über "Google Forms" ausgefüllt.Ergebnisse: 68,7% der Teilnehmer stimmen der Impfung gegen COVID-19 zu. Die univariate Analyse ergab, dass die Altersgruppe der 50- bis 59-Jährigen, Stadtbewohner, Beschäftigte im Gesundheitswesen, Nichtraucher und chronisch Kranke sowie diejenigen, die gegen Influenza, Lungenentzündung und Tetanus geimpft waren, alle bereit waren, sich gegen COVID-19 impfen zu lassen.Schlussfolgerung: Es ist wichtig, die Bereitschaft einer Gemeinschaft zu ermitteln, sich gegen COVID-19 impfen zu lassen, damit Maßnahmen zur Lösung der damit verbundenen Probleme ergriffen werden können. Das Expositionsrisiko und die Erkennung von Gefahren spielen eine entscheidende Rolle für die Akzeptanz der Impfung.
    Subject(s): Covid-19 ; vaccine ; risk ; protectionimmunization ; Covid-19 ; Impfstoff ; Risiko ; Schutz ; Impfung ; ddc: 610
    Language: English
    Type: article
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  • 28
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Audiologie - Audiological Acoustics; VOL: 5; DOC02 /20230120/
    Publication Date: 2023-01-21
    Subject(s): ddc: 610
    Language: German
    Type: article
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  • 29
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    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Audiologie - Audiological Acoustics; VOL: 5; DOC01 /20230120/
    Publication Date: 2023-01-21
    Description: When hearing fails, cochlear implants (CIs) provide most CI users with open speech understanding in quiet environments. CIs bypass the defective sensory organ and electrically stimulate the auditory nerve. The bottleneck of hearing rehabilitation with currently available CIs is their limitation in encoding spectral information resulting from the broad current spread from each electrode contact. Because light can be confined in space more conveniently, optogenetic stimulation of the auditory nerve represents a promising alternative. The development of optogenetic stimulation for future optical CIs requires efforts to design and characterize appropriate optogenetic actuators, viral gene transfer to neurons, and development of multichannel optical CIs. This review article reports the current status of optogenetic stimulation of the auditory pathway and recent breakthroughs in achieving high temporal precision and frequency resolution and in establishing multichannel optical CIs.
    Description: Wenn das Hören versagt, bieten Cochlea-Implantate (CIs) den meisten der CI-Träger ein offenes Sprachverstehen in ruhiger Umgebung. CIs umgehen das defekte Sinnesorgan und stimulieren den Hörnerv elektrisch. Der Flaschenhals der Hörrehabilitation mit aktuell verfügbaren CIs ist deren Limitierung bei der Kodierung spektraler Information, die sich aus der breiten Stromausbreitung von jedem Elektrodenkontakt ergibt. Weil Licht räumlich besser begrenzt werden kann, stellt die optische Stimulation des Hörnervs eine vielversprechende Perspektive für einen grundlegenden Fortschritt der CIs dar. Die Entwicklung der optogenetischen Stimulation für zukünftige optische CIs erfordert Anstrengungen zum Design und zur Charakterisierung geeigneter optogenetischer Aktuatoren, zum viralen Gentransfer auf die Neuronen sowie zur Entwicklung mehrkanaliger optischer CIs. Dieser Übersichtsartikel berichtet über den aktuellen Stand der optogenetischen Stimulation der Hörbahn und über die jüngsten Durchbrüche bei der Erzielung hoher zeitlicher Präzision und Frequenzauflösung sowie bei der Etablierung optischer Mehrkanal-CIs.
    Subject(s): ddc: 610
    Language: German
    Type: article
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  • 30
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    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC02 /20230123/
    Publication Date: 2023-01-24
    Description: The approval of ethanol by the Biocidal Products Regulation has been under evaluation since 2007 due to controversial opinions on the risk assessment. Because of this critical situation, 2022 a memorandum was published to verify whether the use of ethanol for hand antisepsis poses any hazard. On the basis of the memorandum a toxicological evaluation of ethanol-based hand rubs is given.
    Description: Die Bewertung von Ethanol als Biozid gemäß der Biozid-Verordnung wird seit 2007 aufgrund kontroverser Meinungen zur Risikobewertung geprüft. Wegen dieser kritischen Situation wurde 2022 ein Memorandum veröffentlicht, um zu überprüfen, ob die Verwendung von Ethanol zur Händedesinfektion eine Gefahr darstellt. Auf der Grundlage des Memorandums wird eine zusammenfassende toxikologische Bewertung Ethanol basierten Händedesinfektionsmittel gegeben.
    Subject(s): biocidal product regulation ; 1-propanol ; 2-propanol ; ethanol ; ethanol based hand rubs ; benefit-risk-assessment ; virucidal efficacy ; dermal absorption ; worker safety ; patient safety ; Biozid-Verordnung ; 1-Propanol ; 2-Propanol ; Ethanol ; Ethanol basierte Händedesinfektionsmittel ; Nutzen-Risiko-Bewertung ; Viruzidie ; dermale Resorption ; Mitarbeitersicherheit ; Patientensicherheit ; ddc: 610
    Language: English
    Type: article
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  • 31
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Journal of Arts Therapies; VOL: 5; DOC01 /20230125/
    Publication Date: 2023-01-26
    Description: Due to the challenges posed by the COVID-19 pandemic, it has become essential to explore new and innovative ways to conduct art therapy interventions. Digital art therapy is a new way of therapy for both, art therapists and clients. In Spain, concepts for digital art therapy have already been developed and implemented not only by art therapists, but also by organisations and universities. These concepts are aimed at art therapists as part of clinical supervision, as well as at clients receiving art therapy. This paper looks at the rapidly growing area of digitally conducted art therapies and the requirements in relation to infrastructure and security, as well as the challenges and opportunities of this new way of working.
    Description: Sowohl für Kunsttherapeut:innen als auch für die Teilnehmenden ist die digitale Kunsttherapie ein noch ungewohntes Terrain. Aufgrund der COVID-19 Pandemie war es jedoch notwendig, neue und innovative Ansätze zur Durchführung von Kunsttherapie zu erarbeiten. In Spanien werden nicht nur von Kunsttherapeut:innen, sondern auch von Organisationen und Hochschulen Konzepte für die digitale Kunsttherapie durchgeführt. Diese richten sich sowohl an Kunsttherapeut:innen in Form von Supervision und Weiterbildung, als auch an Klient:innen in Form einer Therapiemaßnahme. Die vorliegende Arbeit befasst sich mit diesem rasant wachsenden Arbeitsfeld im Bereich Künstlerischer Therapien und stellt mögliche Setting- und Rahmenbedingungen, Herausforderungen sowie Möglichkeiten für die digitale Kunsttherapie zusammenfassend dar.
    Subject(s): art therapies ; digital space ; online art therapy ; Spain ; COVID-19 ; Künstlerische Therapien ; digitaler Raum ; Online-Kunsttherapie ; Spanien ; COVID-19 ; ddc: 610
    Language: German
    Type: article
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  • 32
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC04 /20230127/
    Publication Date: 2023-01-28
    Description: Objective: The risk of peripheral venous catheter (PVC) infections in inpatients is often underestimated, even if it is lower than that for central venous catheters. Guidelines for the prevention of PVC-associated infections describe the evidence-based management of PVCs. The aims of this study were the development of standardized methods for compliance assessment regarding PVC management and the evaluation of self-reported knowledge and implementations among healthcare providers regarding PVC care.Method: We developed a checklist based on the recommendation of the Commission of Hospital Hygiene and Infection Prevention at the Robert Koch Institute (KRINKO) Berlin for the standardized evaluation of PVC management. The following parameters were collected and evaluated: condition of the puncture site, condition of the bandage, presence of an extension set, presence of a plug, and documentation. The checklist was applied in 14 normal wards in 2019. After feedback of the ward staff on the results, it was applied again in 2020 in the same wards. For retrospective data analysis, we used a newly developed PVC-quality index. After the second evaluation in 2020, we carried out an anonymous survey among the healthcare providers.Results: The evaluation of 627 indwelling PVCs showed a significant increase in compliance related to the presence of an extension set (p=0.049) and documentation (p〈0.001) in the 2nd year. The quality index increased in 12 out of 14 wards. The participants of the survey were aware of the in-house standard "Prevention of vascular catheter-associated infections", with a mean score of 4.98 on a Likert scale (1=not aware, 7=completely aware). The main barrier to implementation of the preventive measures was the time factor. Survey participants were more aware of PVC placement than PVC care.Conclusion: The PVC quality index is a valuable tool for the assessment of compliance regarding PVC management in daily practice. Feedback from the ward staff on the results of compliance assessment improves PVC management, but the outcome is very heterogeneous.
    Description: Zielsetzung: Das Infektionsrisiko von peripheren Venenkathetern (PVK) bei stationären Patienten wird, auch wenn es niedriger ist als das von zentralen Venenkathetern, häufig unterschätzt. Leitlinien zur Prävention von PVK-assoziierten Infektionen beinhalten Evidenz-basierte Maßnahmen zur Anlage, Pflege und Nutzung von liegenden PVK. Ziel dieser Studie war, ein Verfahren zur standardisierten Bewertung der Compliance im Umgang mit liegenden PVK zu entwickeln und zu testen. Außerdem sollte eine Selbsteinschätzung des Personals zu Kenntnis und Umsetzung der Präventionsmaßnahmen erhoben werden.Methode: Basierend auf den KRINKO-Empfehlungen wurde eine Checkliste zur standardisierten Bewertung der Umsetzung von Präventionsmaßnahmen bei liegenden PVK entwickelt. Folgende Parameter wurden erhoben und bewertet: Zustand der Punktionsstelle, Zustand des Verbands, Vorhandensein eines Extensionssets, Verschluss, Nutzung und Dokumentation der Maßnahmen. Die Checkliste wurde erstmals im Jahr 2019 auf 14 Normalstationen angewendet und nach Feedback der Ergebnisse erneut im Jahr 2020 auf denselben Stationen eingesetzt. Mithilfe eines neu entwickelten PVK-Qualitätsindex erfolgte ein retrospektiver Vergleich. Nach der 2. Bewertung wurde eine anonyme Mitarbeiterbefragung mittels Fragebogen durchgeführt.Ergebnisse: Bei der Bewertung von 627 liegenden PVK zeigte sich eine signifikante Steigerung der Compliance bezogen auf das Vorhandensein eines Extensionssets (p=0,049) und die Dokumentation (p〈0,001) im 2. Jahr. Der Qualitätsindex ist auf 12 der 14 beobachteten Stationen gestiegen. Die Bekanntheit einzelner Maßnahmen des hausinternen Standards "Prävention gefäßkatheterassoziierter Infektionen" wurde mit dem Mittelwert 4,98 von 7 auf einer Likert-Skala angegeben; als einziges Hindernis in der Umsetzung konnte der Faktor Zeit identifiziert werden. Maßnahmen bei PVK-Anlage waren zu einem höheren Anteil bekannt als Maßnahmen der PVK-Pflege. Fazit: Der PVK-Qualitätsindex ist erfolgreich angewendet worden und ist geeignet, die PVK-bezogene Compliance standardisiert zu erheben. Feedback der Compliance im Umgang mit PVK hat diese verbessert, aber der Effekt ist sehr heterogen.
    Subject(s): catheter related infection ; peripheral venous catheter ; compliance assessment ; compliance self-assessment ; peripheral venous catheter quality index ; Katheter-assoziierte Infektion ; peripherer Venenkatheter ; Compliance-Bewertung ; Compliance-Selbsteinschätzung ; PVK-Qualitätsindex ; ddc: 610
    Language: English
    Type: article
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  • 33
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Hygiene and Infection Control; VOL: 18; DOC03 /20230127/
    Publication Date: 2023-01-28
    Description: Introduction: There is a risk of transmission of viruses and microbial pathogens during routine health care procedures due to improper injection, infusion, and medication-vial practices. Unsafe practices lead to outbreaks of infection resulting in unacceptable and devastating events in patients. The present study was undertaken to assess the compliance of nurses with safe injection and infusion practices in our hospital and to identify staff education requirements in relation to the safe-injection and infusion practices policy.Methods: Baseline data were collected and high risk areas were identified on this basis, a quality improvement project was implemented by infection control team. FOCUS PDCA methodology was used to conduct the improvement process. The study was performed from March to September 2021. An audit checklist based on the CDC guidelines was used for monitoring compliance with safe injection and infusion practices. Results: Poor compliance with safe injection and infusion practices in few clinical areas at baseline. During the pre-intervention period, non-compliance was mainly seen with the following elements: aseptic technique (79%), rubber septum disinfected with alcohol (66%), labelling of all IV lines and medications with date and time (83%), compliance with multidose-vial policy (77%), use of multidose vials for single patient (84%), safe disposal of sharps (84%), using trays instead of clothing/pockets to carry medications (81%).There was significant improvement in compliance with the following elements of safe injection and infusion practices in the post-intervention period: aseptic technique (94%), rubber septum disinfected with alcohol (83%), compliance with multidose-vial policy (96%), use of multidose vials for single patient only (98%), safe disposal of sharps (96%).Conclusion: Adherence to safe injection and infusion practices is very important to prevent outbreaks of infection in health care settings.
    Description: Einführung: Im Fall unsachgemäßer Injektion, Infusion und fehlerhaften Umgangs mit Medikamenten besteht das Risiko der Übertragung von Viren und Mikroorganismen. Fehlerhafte Praktiken können zum Ausbruch von Infektionen mit drastischen Folgen für die Patienten führen. Die vorliegende Studie wurde durchgeführt, um die Einhaltung sicherer Injektions- und Infusionspraktiken in unserem Krankenhaus durch das Pflegepersonal zu bewerten und den Schulungsbedarf des Personals zu ermitteln.Methoden: Die Analyse der Ausgangssituation ergab eine schlechte Einhaltung sicherer Injektions- und Infusionspraktiken in einigen klinischen Bereichen. In den identifizierten Bereichen mit hohem Risiko wurde vom Infektionskontrollteam ein Qualitätsverbesserungsprojekt etabliert. Zur Durchführung wurde die FOCUS PDCA-Methode verwendet. Die Studie erstreckte sich von März bis September 2021. Zur Überwachung der Einhaltung sicherer Injektions- und Infusionspraktiken wurde eine Audit-Checkliste auf der Grundlage der CDC-Richtlinien verwendet.Ergebnisse: Während der Zeit vor der Intervention wurde die Nichteinhaltung hauptsächlich in Bezug auf folgende Elemente festgestellt: Aseptische Technik (79%), mit Alkohol desinfiziertes Gummiseptum (66%), Kennzeichnung aller Infusionsleitungen und Medikamente mit Datum und Uhrzeit (83%), Einhaltung der Richtlinie für Mehrdosisfläschchen (77%), Verwendung von Mehrdosisfläschchen für einen einzelnen Patienten (84%), sichere Entsorgung von scharfen Gegenständen (84%), Tragen von Medikamenten in Kleidung/Taschen anstatt auf dem Tablett (81%).Es gab eine signifikante Verbesserung bei der Einhaltung der folgenden Elemente sicherer Injektions- und Infusionspraktiken nach der Intervention: Aseptische Technik (94%), mit Alkohol desinfiziertes Gummiseptum (83%), Einhaltung der Richtlinie für Mehrdosis-Durchstechflaschen (96%), Verwendung von Mehrdosis-Durchstechflaschen für einen einzelnen Patienten (98%), sichere Entsorgung scharfer Gegenstände (96%).Schlussfolgerung: Die Einhaltung sicherer Injektions- und Infusionspraktiken ist unerlässlich, um Ausbrüche nosokomialer Infektionen zu verhindern.
    Subject(s): safe injection and infusion practices ; aseptic techniques ; single syringe ; single needle ; single patient ; multidose-vial policy ; labelling of IV lines ; safe disposal of sharps ; sichere Injektions- und Infusionspraktiken ; aseptische Technik ; Einzelspritze ; Einzelnadel ; Einzelpatient ; Mehrdosisfläschchen ; Kennzeichnung von Infusionsleitungen ; sichere Entsorgung scharfer Gegenstände ; ddc: 610
    Language: English
    Type: article
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  • 34
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC05 /20230130/
    Publication Date: 2023-01-31
    Description: Purpose: Vicarious menstruation is cyclical bleeding in extra-uterine locations that occurs during menstruation or within 48 h of its onset. We aim to present a 43-year-old female with ocular vicarious menstruation, its treatment, and a review of other published cases of ocular vicarious menstruation. Case presentation: A 43-year-old Caucasian female presented with a 15-year history of recurrent monthly unilateral subconjunctival hemorrhage. The episodes were cyclical and coincided with the onset of menses, lasting for approximately 10 to 14 days. Slit-lamp examination of the right eye showed nasally located subconjunctival hemorrhage. Detailed laboratory findings, including parameters for various hematological disorders, were normal. A follow-up examination 2 weeks later showed that the subconjunctival hemorrhage in the right eye was completely resolved. The patient was prescribed the oral contraceptive levonorgestrel/ethinyl estradiol and marked improvement at the recurrences of subconjunctival hemorrhage was noted during subsequent menses.Conclusion: Ocular vicarious menstruation is among the rarest causes of recurrent subconjunctival hemorrhage. A therapeutic trial of oral contraceptive should be considered in patients that present with ocular vicarious menstruation.
    Subject(s): recurrent subconjunctival hemorrhage ; ocular vicarious menstruation ; extrauterine bleeding ; oral contraceptive pills ; ddc: 610
    Language: English
    Type: article
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  • 35
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC04 /20230130/
    Publication Date: 2023-01-31
    Description: Objective: Surgically induced scleral necrosis (SISN) is a potentially blinding sequela that may occur after any ocular procedure. SISN in the context of active tuberculosis is seldom seen. We report a case of a patient with asymptomatic tuberculosis who developed SISN after pterygium surgery.Methods: A 76-year-old Mexican-mestizo woman from Veracruz, Mexico, was referred to our clinic because of severe disabling pain and scleral thinning in her right eye.Results: Tubercular-related SISN was finally diagnosed and managed successfully with antitubercular therapy, topical and systemic corticosteroidsConclusion: Tuberculosis must be considered as a differential diagnosis of high-risk patients in the context of refractory SISN in endemic countries.
    Subject(s): antitubercular therapy ; infectious scleritis ; necrotizing scleritis ; ocular tuberculosis ; pterygium surgery ; scleral inflammation ; surgically induced scleral necrosis ; ddc: 610
    Language: English
    Type: article
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  • 36
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Audiologie - Audiological Acoustics; VOL: 5; DOC03 /20230131/
    Publication Date: 2023-02-01
    Description: The present study investigated whether direct bilateral streaming on cochlea implant (CI) and hearing aid provides bimodal hearing instrument users with an advantage when making phone calls via smartphone compared with previously used methods.In two laboratory tests, 22 experienced CI listeners (mean age 50.8±18.6 years), fitted bimodally with a Cochlear N7 processor, as well as a GN ReSound hearing aid, were tested with their own previously used, mostly unilateral phone use (reference condition) and with MFi technology (test condition). In the test condition, signals were directly streamed from a smartphone to both hearing instruments. Speech intelligibility, subjectively perceived listening effort, each in interfering noise, and subjective ratings (including sound, usability via System Usability Scale, SUS) were obtained as key measures of benefit. The results showed a trend towards improved speech intelligibility, as well as a significantly lower listening effort with bilateral streaming compared to conventional telephone use in both the measurement and the questionnaire data. In terms of usability, bilateral streaming also resulted in better ratings compared to the reference.
    Description: In der vorliegenden Studie wurde untersucht, ob das direkte bilaterale Streamen in Cochlea-Implantat (CI) und Hörgerät (HG) bimodalen Hörsystemnutzern einen Vorteil beim Telefonieren mit dem Smartphone im Vergleich zu bisher genutzten Verfahren bringt. In zwei Labortests wurden 22 erfahrene CI-Träger (Durchschnittsalter 50,8±18,6 Jahre), die bimodal mit einem N7 Prozessor der Firma Cochlear sowie einem GN ReSound Hörgerät versorgt waren, mit ihrer eigenen bisher genutzten, meist unilateralen Telefonnutzung (Referenz-Kondition) sowie mit MFi-Technologie (Test-Kondition) getestet. In der Test-Kondition wurde direkt von einem Smartphone auf beide Hörsysteme gestreamt. Als wesentliche Messgrößen des Benefits wurden die Sprachverständlichkeit, die subjektiv empfundene Höranstrengung, jeweils im Störgeräusch, und subjektive Bewertungen (u.a. zu Klang, Gebrauchstauglichkeit über System Usability Scale, SUS) erfasst. Die Ergebnisse ergaben eine tendenziell verbesserte Sprachverständlichkeit, sowie eine signifikant geringere Höranstrengung bei beidseitigem Streaming im Vergleich zur herkömmlichen Telefonnutzung in sowohl den Mess-, als auch den Fragebogendaten. Im Hinblick auf Gebrauchstauglichkeit führte das beidseitige Streaming, verglichen mit der Referenz, ebenfalls zu besseren Bewertungen.
    Subject(s): cochlear implant ; MFi ; streaming ; telephoning with CI ; Cochlea-Implantat ; MFi ; Streamen ; Telefonieren mit CI ; ddc: 610
    Language: German
    Type: article
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  • 37
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC02 /20230130/
    Publication Date: 2023-01-31
    Description: Gelatinous drop-like corneal dystrophy (GDLD) is a rare autosomal recessive corneal dystrophy that has been associated with mutations in the TACSTD2 (M1S1) gene, which is normally expressed in corneal epithelial cells. GDLD is characterized by progressive deposition of amyloid in the corneal stroma with rapid recurrence in grafts after penetrating keratoplasty. We report of case of a patient with GDLD treated bilaterally with staged limbal stem cell transplantation and penetrating keratoplasty that resulted in long-term control of his disease. This case demonstrates that staged allogenic limbal stem cell transplantation, before or after penetrating keratoplasty, can be used to restore vision long-term in GDLD patients.
    Subject(s): gelatinous drop-like corneal dystrophy (GDLD) ; penetrating keratoplasty (PKP) ; allogeneic limbal stem-cell transplantation ; ddc: 610
    Language: English
    Type: article
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  • 38
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Ophthalmology Cases; VOL: 13; DOC01 /20230130/
    Publication Date: 2023-01-31
    Description: Astrocytic hamartoma is a benign glial tumor. It may be associated with tuberous sclerosis and can also be found incidentally on retinal examination as an isolated presentation. Here, we describe multimodal imaging characteristics of astrocytic hamartoma in a patient with retinitis pigmentosa. Spectral domain optical coherence tomography of both eyes showed moth-eaten optically empty spaces and hyperreflective dots along with foveal thinning. Multicolor image highlighted mulberry appearance of the lesion with green shift signifying elevated lesion. In infrared reflectance, lesion was hyporeflective with its margins well delineated. Green reflectance and blue reflectance highlighted calcification as multiple hyperreflective dots. Autofluorescence showed typical hyperautofluorescence.
    Subject(s): astrocytic hamartoma ; multicolor imaging ; retinitis pigmentosa ; ddc: 610
    Language: English
    Type: article
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  • 39
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Journal of Arts Therapies; VOL: 5; DOC02 /20230228/
    Publication Date: 2023-02-28
    Description: The definition of a therapeutic discipline provides an important position-fixing and offers orientation for patients, interested parties and professional actors in health care. The definition of music therapy (MT) serves both the self-portrayal of music therapists and the professional classification in the institutions of the public health care system. Thus it contributes decisively to the professional political profiling. There is currently no definition of music therapy in Germany that fits well into the German health system.In this article, different definitions are presented and critically questioned. Against the background of new developments within MT, the necessity for a new definition is shown. This is presented and explained in its conceptual components.
    Description: Die Definition einer therapeutischen Disziplin leistet eine wichtige Standortbestimmung und bietet Orientierung für Patient:innen, Interessent:innen und professionelle Akteure der Gesundheitsversorgung. Die Definition von Musiktherapie (MT) dient sowohl der Selbstdarstellung von Musiktherapeut:innen, als auch der fachlichen Einordnung in die Institutionen des öffentlichen Gesundheitswesens. Damit trägt sie entscheidend zur berufspolitischen Profilierung bei. Es gibt aktuell in Deutschland keine im deutschen Gesundheitssystem gut anschlussfähige Definition von Musiktherapie. In diesem Artikel werden verschiedene Definitionen dargestellt und kritisch hinterfragt. Vor dem Hintergrund von Neuentwicklungen innerhalb der MT wird die Notwendigkeit für eine neue Definition aufgezeigt. Diese wird vorgestellt und in ihren Begriffsbausteinen erläutert.
    Subject(s): definition ; music therapy ; intervention ; methods ; Definition ; Musiktherapie ; Intervention ; Methodik ; ddc: 610
    Language: German
    Type: article
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  • 40
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Medizinische Informatik, Biometrie und Epidemiologie; VOL: 19; DOC13 /20230912/
    Publication Date: 2023-09-12
    Subject(s): ddc: 610
    Language: German
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  • 41
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Medizinische Informatik, Biometrie und Epidemiologie; VOL: 19; DOC12 /20230912/
    Publication Date: 2023-09-12
    Description: Health kiosks are basically a low-threshold opportunity for communication between citizens and other actors in the healthcare system as well as for information on health-related issues. The aim of the study was to record the use of health kiosks in pharmacies by means of a quantitative long-term survey and to determine the reasons for (non-)use by means of a qualitative in-depth analysis. Overall, usage parameters were recorded over 25 months in log files of up to 145 pharmacies. In addition, 92 customers of four high-usage pharmacies were interviewed in qualitative short interviews regarding the reasons for use or non-use. The results of the long-term study showed that the health kiosks were used 1-2 times a day at best, but more likely only 1-2 times a week. The reasons for non-use were due to the interaction of different factors, including accessibility and usefulness, but also the social environment. It can be concluded that identifying the reasons for (non-)use is an important step towards allowing the potential of health kiosks to unfold if they are to be used in a targeted manner within the framework of the digitalisation strategy of the Federal Ministry of Health.
    Description: Gesundheitskioske stellen grundsätzlich eine niedrigschwellige Möglichkeit für die Kommunikation zwischen Bürgern und weiteren Akteuren im Gesundheitswesen sowie für die Information zu gesundheitsrelevanten Fragestellungen dar. Ziel der Studie war es, über eine quantitative Langzeiterhebung die Nutzung von Gesundheitskiosken in Apotheken zu erfassen und über eine qualitative Tiefenanalyse die Gründe für die (Nicht)-Nutzung zu ermitteln. Insgesamt wurden Nutzungsparameter über 25 Monate in Logdateien von bis zu 145 Apotheken festgehalten. Darüber hinaus wurden 92 Kunden von vier nutzungsstarken Apotheken in qualitativen Kurzinterviews hinsichtlich der Gründe der Nutzung bzw. Nichtnutzung befragt. Die Ergebnisse der Langzeitstudie zeigen, dass die Gesundheitskioske im besten Falle 1-2 mal pro Tag, eher aber nur 1-2 mal pro Woche genutzt wurden. Die Gründe für die Nicht-Nutzung wurden durch das Zusammenspiel unterschiedlicher Faktoren bedingt, hierzu zählten u.a. die Zugänglichkeit und die Nützlichkeit, aber auch das soziale Umfeld. Zusammenfassend lässt sich feststellen, dass die Identifizierung der Gründe für die (Nicht-)Nutzung ein wichtiger Schritt ist, damit sich das Potential von Gesundheitskiosks entfalten kann, wenn sie im Rahmen der Digitalisierungsstrategie des Bundesministeriums für Gesundheit zum gezielten Einsatz kommen sollen.
    Subject(s): self-service technology ; SST ; health kiosk ; usage ; usefulness ; Selbstbedienungstechnologien ; Gesundheitskiosk ; Nutzung ; Nutzen ; ddc: 610
    Language: German
    Type: article
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  • 42
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Medizinische Informatik, Biometrie und Epidemiologie; VOL: 19; DOC11 /20230912/
    Publication Date: 2023-09-12
    Description: Introduction: According to the Master Plan 2020, science education will play a critical role in future medical curricula. Science modules have already been implemented at many locations. Other medical faculties will follow in the next few years, as legislation is expected to make recommendations of the national competence-based learning objectives curriculum for medicine (NKLM) mandatory. This article aims to present an implementation example from epidemiology and biometry as a contribution to the didactic discussions within the data sciences in medicine. Project description: We report on our experiences with a data analysis project for second-year medical students, which has been compulsory at the Faculty of Medicine and Health Sciences since 2019. The project is intended to train the scientific skills required from the subjects of epidemiology and biometry for student research projects. Emphasis is placed on responsible data handling, transparency, and reproducibility. For example, the writing of a statistical analysis plan is required prior to data access. Improved standardization of materials, optional use of the English language, and digital support will be implemented to help manage the project when student numbers increase. Discussion: The experience from five years is very positive, although a formal evaluation of the learning success is still pending. Current challenges concern staffing, additional time and supervision requirements for those students who do statistical programming with R, and improved integration into the medical curriculum.
    Description: Einleitung: Wissenschaftsausbildung wird im Medizinstudium gemäß Masterplan 2020 künftig einen höheren Stellenwert einnehmen. An vielen Standorten sind wissenschaftliche Curricula bereits umgesetzt. Andere Fakultäten werden voraussichtlich in den nächsten Jahren nachziehen und dabei auch die Empfehlungen des Nationalen Kompetenzbasierten Lernzielkatalogs Medizin (NKLM) zu berücksichtigen haben. Ziel dieses Artikels ist die Darstellung eines Umsetzungsbeispiels aus den Fächern Epidemiologie und Biometrie als Beitrag zur fachlich-didaktischen Diskussion. Projektbeschreibung: Wir berichten von unseren Erfahrungen mit einem seit 2019 an der Fakultät für Medizin und Gesundheitswissenschaften verpflichtenden Datenanalyseprojekt für Medizinstudenten im zweiten Studienjahr. Das Projekt soll die aus den Fächern Epidemiologie und Biometrie benötigten wissenschaftlichen Kompetenzen für spätere eigene Forschungsprojekte trainieren. Dabei wird besonderer Wert auf verantwortungsvollen Umgang mit Daten, Transparenz und Reproduzierbarkeit gelegt. Beispielsweise stellt ein von den Studenten zu erstellender statistischer Analyseplan eine notwendige Voraussetzung des Datenzugangs dar. Parallel werden eine zunehmende Standardisierung der Betreuung, wahlweise Nutzung von Englisch und digitale Unterstützung umgesetzt, damit das Projekt perspektivisch auch bei ansteigenden Studentenzahlen zu bewältigen ist. Diskussion: Die Erfahrungen aus bisher 5 Jahren sind sehr positiv, wobei eine formale Evaluation des Lernerfolgs noch aussteht. Das Projekt ist sowohl für die Studenten als auch für Betreuer und Dozenten aufwändiger als derzeit abgebildet werden kann. Aktuelle Herausforderungen bestehen vor allem hinsichtlich personeller Ausstattung, zusätzlichen Zeit- und Betreuungsbedarfs derjenigen Studenten, die sich für eigene statistische Programmierung mit R entscheiden, und einer Optimierung der curricularen Einbindung.
    Subject(s): science education ; medical curriculum ; teaching goals ; epidemiology and biometry ; Wissenschaftsausbildung ; Medizinstudium ; Lernzielkatalog ; Epidemiologie und Biometrie ; ddc: 610
    Language: German
    Type: article
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  • 43
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW; VOL: 12; DOC10 /20231031/
    Publication Date: 2023-10-31
    Description: The German guideline for oral squamous cell carcinoma (OSCC) describes the recommended diagnosis and treatment procedures for OSCC and ensures the highest quality patient care. However, the current German guideline for OSCC is indistinct and therapy planning is not standardized in detail between centers. To address this, the current diagnostic and therapeutic strategies in different oral and maxillofacial surgery departments in Germany were summarized using a uniform questionnaire. The results revealed high standards in oncologic maxillofacial care, but non-uniform standards exist between centers. Moreover, an increasing use of diagnostic and treatment methods that are not included in the German guideline for OSCC, such as positron emission tomography computed tomography (PET-CT) and tumor biomarkers, were used by different centers. These results support the updated German guideline for OSCC but highlight the need to consider other additive methods to improve patient care and outcomes. Furthermore, a recommendation to introduce tumor passports to simplify OSCC diagnosis and treatment should be discussed. These changes will improve the prognosis and quality of life of patients with OSCC.
    Description: Die deutsche Leitlinie zum oralen Plattenepithelkarzinom beschreibt die empfohlenen Diagnose- und Behandlungsverfahren und gewährleistet eine qualitativ hochwertige Patientenversorgung. Allerdings ist die aktuelle Leitlinie zum oralen Plattenepithelkarzinom unklar und die Therapieplanung nicht im Detail zwischen den Zentren standardisiert. Um diesem Problem Rechnung zu tragen, wurden die aktuellen Diagnose- und Therapiestrategien in verschiedenen Abteilungen der Mund-, Kiefer- und Gesichtschirurgie in Deutschland anhand eines einheitlichen Fragebogens zusammengefasst. Die Ergebnisse zeigten hohe Standards in der onkologischen Betreuung, es bestehen jedoch uneinheitliche Standards zwischen den Zentren. Darüber hinaus wurden in verschiedenen Zentren zunehmend Diagnose- und Behandlungsmethoden eingesetzt, die nicht in der Leitlinie für orale Plattenepithelkarzinome enthalten sind, wie z.B. die Positronenemissionstomographie in Kombination mit der Computertomographie (PET-CT) und Tumorbiomarker. Diese Ergebnisse unterstützen die aktualisierte Leitlinie für orale Plattenepithelkarzinome, unterstreichen jedoch die Notwendigkeit, andere additive Methoden in Betracht zu ziehen, um die Patientenversorgung und die Ergebnisse zu verbessern. Darüber hinaus sollte eine Empfehlung zur Einführung von Tumorpässen zur Vereinfachung der oralen Plattenepithelkarzinom-Diagnose und -Behandlung diskutiert werden. Diese Änderungen werden die Prognose und Lebensqualität von Patienten mit oralen Plattenepithelkarzinom verbessern.
    Subject(s): OSCC ; German guideline for OSCC ; therapeutic options ; diagnostic standards ; tumor passport ; questionnaire ; ddc: 610
    Language: English
    Type: article
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  • 44
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW; VOL: 12; DOC09 /20231031/
    Publication Date: 2023-10-31
    Description: Objective: To analyze the effects of Argireline on skin surface wrinkles using the Visia® camera system developed by Canfield Scientific Inc., U.S.A., for facial image capture. Method: Nineteen female participants were recruited from a plastic surgery clinic. Initial facial images captured the left, front, and right sides of the participants' faces, which were documented as timepoint one. Following this, the participants immediately began to apply a facial skin serum containing triple hyaluronic acids produced by CNC cosmetic GmbH, Philippsburg, Germany. The serum was applied once in the morning and once in the evening. Participants received two identical containers labeled L for left and R for right, with each container to be used on the corresponding facial side, particularly around the eye area. One container contained Argireline, a synthetic hexapeptide, which previously was deemed to be a biosafe alternative to botulinum neurotoxin. The study was conducted as double-blind; neither the participants nor researchers knew which of the two containers contained Argireline. Participants were allowed to use their own cosmetic products throughout the study. After four weeks, the participants returned to have their faces recaptured using the Visia® camera, which was documented as timepoint two. The absolute scores of the wrinkles were noted, and results on both sides of the face were calculated and compared. The "TruSkinAge®" measurement provided by the Visia® camera was reviewed for each face side. Results between both time points and both sides of the face were compared. After the data analysis was complete, the company was contacted to determine which container contained Argireline.Results: Nineteen participants returned for facial image capture. There were no significant adverse events, allergic reactions, or skin irritations. The investigation revealed that the wrinkle score slightly decreased for the right and left side of the face following four weeks of serum application. However, this decrease was not significant (p〉0.05) based on the Wilcoxon matched pairs tests for the wrinkle scores (right side p=0.060 and left side p=0.176) and Truskin Ages® results (right side p=0.096 and left side p=0.489).Comparing the data from the right side with that from the left side of the face revealed that neither demonstrated a significant reduction in wrinkle score (p=0.829) or Truskin Ages® results (p=0.804). Argireline was included in the serum applied to the right side of the face. However, no statistical significance was seen in the results on this side of the face indicating any possible effects. Conclusion: Wrinkle scores and Truskin Ages® results were observed to decrease non-significantly following the application of a skin serum involving hyaluronic acid. The Visia® imaging method was used to analyze the data objectively. Differences between both sides of the face that were treated with and without Argireline were not statistically significant. Therefore, the effect of Argireline was not proven. While Argireline presented with low toxicity, its efficacy was found not to be significant. Therefore, it is not deemed to be an alternative treatment to botulinum toxin.
    Description: Ziel: Objektive Analyse der Effekte von Argireline auf Falten der Hautoberfläche durch Anwendung der Visia® Kamera der Firma Canfield Scientific Inc., U.S.A., für Bildaufnahmen des Gesichtes. Methode: Neunzehn weibliche Freiwillige wurden aus einer Plastisch-Chirurgischen Klinik rekrutiert. Initiale Bildaufnahmen der Gesichter von links, vorne und rechts wurden aufgenommen, was als Zeitpunkt Eins dokumentiert wurde. Danach starteten die Freiwilligen umgehend mit der Anwendung eines Hautpflegeserums, welches dreifache Hyaluronsäuren für das Gesicht enthielt, produziert durch die CNC cosmetic GmbH, Philippsburg, Deutschland. Das Serum wurde einmal morgens und einmal abends angewendet. Die Teilnehmer erhielten zwei identische Töpfchen, die mit L für links und R für rechts bezeichnet wurden; entsprechend sollten sie auf der jeweiligen Seite des gereinigten Gesichtes, besonders rund um die Augenpartie, angewendet werden. Ein Töpfchen enthielt Argireline, ein synthetisches Hexapeptid, welches zuvor als biologisch sichere Alternative zu Botulinum Neurotoxin beurteilt worden war. Die Studie wurde doppelt blind durchgeführt, wobei weder die Freiwilligen noch die Forscher wussten, welches der beiden Töpfchen das Argireline enthielt. Die Freiwilligen durften zusätzlich ihre eigenen kosmetischen Produkte während der Studie benutzen. Nach vier Wochen kamen die Teilnehmer zurück, um das Gesicht erneut mittels der Visia® Kamera fotografiert zu bekommen, was als Zeitpunkt Zwei vermerkt wurde. Die absoluten Scores der Falten wurden notiert und die Ergebnisse der beiden Seiten des Gesichtes berechnet und verglichen. Ebenfalls wurde das gemessene "TruskinAge®", das von der Visia® Kamera herausgegeben wurde, für jede Gesichtshälfte untersucht. Die Ergebnisse für beide Zeitpunkte und beide Seiten des Gesichtes wurden verglichen. Nachdem die Datenanalyse vollständig war, wurde die Firma kontaktiert, um zu bestimmen, welches Töpfchen das Argireline enthielt. Ergebnisse: Neunzehn Freiwillige kamen für die Gesichtsaufnahmen zurück. Es gab keine signifikanten Nebenwirkungen, keine allergischen Reaktionen oder Hautirritationen. Die Untersuchung ergab, dass der Faltenscore für die rechte und linke Seite des Gesichtes nach vier Wochen der Anwendung der Produkte leicht abnahm. Allerdings war die Abnahme nicht signifikant (p〉0.05) gemäß des Wilcoxon-Testes für zwei abhängige Stichproben für die Faltenscores (rechte Seite p=0,060 und linke Seite p=0,176) und für die TruskinAge® Ergebnisse (rechte Seite p=0,096 und linke Seite p=0,489). Beim Vergleich der Daten der rechten mit denen der linken Seite des Gesichtes ergab sich, dass keine der Gesichtshälften signifikant besser abschnitt als die andere in Hinblick auf die Reduzierung der Faltenscores (p=0,829) und der TruskinAge® Ergebnisse (p=0,804). Argireline war im Serum enthalten, das auf der rechten Seite des Gesichtes angewendet wurde. Allerdings wurde keine statistische Signifikanz der Ergebnisse auf dieser Seite des Gesichtes im Hinblick auf mögliche Effekte erreicht. Schlussfolgerung: Die Scores der Falten sowie die Ergebnisse der Truskin Ages® zeigten eine nicht signifikante Tendenz, nach Anwendung des Hautpflegeserums basierend auf Hyaluronsäuren zurückzugehen. Die Visia® Bildgebungsmethode wurde für die objektive Datenanalyse eingesetzt. Die Unterschiede zwischen den beiden Gesichtshälften, die mit und ohne Argireline behandelt wurden, waren nicht statistisch signifikant. Daher wurde die Wirkung von Argireline nicht nachgewiesen. Während sich Argireline mit niedriger Toxizität darstellte, wurden seine Effekte als nicht signifikant ermittelt. Daher wird es nicht als alternative Behandlung zu Botulinumtoxin eingestuft.
    Subject(s): Argireline ; wrinkles ; skin serum with hyaluronic acid ; Truskin Age® ; skin surface features ; Visia® complexion analysis camera system ; objective measurements ; Argireline ; Falten ; Hautserum mit Hyaluronsäuren ; TruSkin Age® ; Hautoberflächenkriterien ; Visia® Kamerasystem für Hautanalyse ; objektive Messungen ; ddc: 610
    Language: English
    Type: article
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  • 45
    Subject(s): Biochemistry. ; Biochemistry.
    In: Springer Nature eBook
    Description / Table of Contents: This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience.Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
    Type of Medium: Online Resource
    Pages: XV, 357 p. 82 illus., 71 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071626757
    Series Statement: Methods in Molecular Biology, 2566
    Language: English
    Note: Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes -- Autofluorescence Label-Free Imaging of Liver Reticular Structure -- Lysosome Imaging Based on Fluorescent Carbon Dots -- Oxidative and Nitrosative Stress Detection in Human Sperm using Fluorescent Probes -- Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis -- Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions -- Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans -- Combined Lectin- and Immuno-Histochemistry (CLIH) for Fluorescence Microscopy -- Immunofluorescence Labelling of Skeletal Muscle in Development, Regeneration, and Disease -- Immunohistochemical Detection of the Autophagy Markers LC3 and P62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissues -- Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin Fixed and Paraffin Embedded Tissues -- Immunogold Labelling of Milk Proteins at Transmission Electron Microscopy -- Rediscover Potassium Permanganate as a Stain for Basic Proteins on Ultrathin Sections at Transmission Electron Microscopy -- Tissue Fixation and Processing for the Histological Identification of Lipids -- Staining Methods for Normal and Regenerative Myelin in the Nervous System -- Nile Red and BODIPY Staining Of Lipid Droplets in Mouse Oocytes and Embryos -- Chromatin Dispersion Test to Assess DNA Damage in Cervical Epithelial Cells -- Uranyl-free staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy -- Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapours -- Localising Molecules in Plant Cell Walls using Fluorescence Microscopy -- Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro -- Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls -- Staining Starch with Iodine Solution -- Histochemical Analysis of Plant Secretory Structures -- Alcian Blue Staining to Visualize Intracellular Hyaluronic-Acid-Based Nanoparticles -- Prussian Blue Staining to Visualize Iron Oxide Nanoparticles -- Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy -- Fluorescent Labelling of Lignin Nanocapsules with Fluorol Yellow 088. .
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  • 46
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Analytical biochemistry. ; Pharmacology. ; Analytical Biochemistry. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
    Type of Medium: Online Resource
    Pages: XVII, 509 p. 138 illus., 65 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071627280
    Series Statement: Methods in Molecular Biology, 2576
    Language: English
    Note: Need for Methods to Investigate Endocannabinoid Signaling -- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues -- Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies -- LC-MS/MS Analysis of AEA and 2-AG -- Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices -- Endocannabinoid-Binding Receptors as Drug Targets -- Assay of CB1 Receptor Binding -- The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Fluorescence-based Assay for TRPV1 Channels -- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding -- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ and PPARδ Receptors -- A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγ -- The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells -- Assay of GTPγS Binding in Autoradiography -- Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2 -- Endocannabinoid Metabolism and Transport as Drug Targets -- Assay of NAT Activity -- Radiometric Assay of NAPE-PLD activity -- A Fluorescence-based NAPE-PLD Activity Assay -- Radiometric Assay of FAAH Activity -- Fluorimetric Assay of FAAH activity -- Assay of NAAA Activity -- Assay of DAGLα/β Activity -- Assay of Monoacylglycerol Lipase Activity -- Radiometric Assay of ABHD2 Activity -- Oxygenation of Anandamide by Lipoxygenases -- Assay of Endocannabinoid Oxidation by Cytochrome P450 -- Assays of Endocannabinoid Uptake -- Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction -- Bioinformatics of the Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene promoter -- DNA Methylation Analysis of Cnr1 Gene Promoter -- Western Blotting of the Endocannabinoid System -- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells -- Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal and Electron Microscopy -- Measuring Endocannabinoid System Interaction with Biomembranes -- STORM Super-Resolution Imaging of CB1 Receptors in Tissue Preparations -- Visualization of Endocannabinoids in the Cell -- Electrophysiology of Endocannabinoid Signalling -- Machine Learning and Computational Chemistry for the Endocannabinoid System -- A Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor.
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  • 47
    Subject(s): Biomaterials. ; Nucleic acids. ; Biology—Technique. ; Biophysics. ; Nucleic Acid. ; Biophysical Methods.
    In: Springer Nature eBook
    Description / Table of Contents: This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.
    Type of Medium: Online Resource
    Pages: XI, 282 p. 78 illus., 66 illus. in color. , online resource.
    Edition: 2nd ed. 2023.
    ISBN: 9781071626955
    Series Statement: Methods in Molecular Biology, 2570
    Language: English
    Note: Automated ssDNA SELEX Using Histidine Tagged Target Proteins -- Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection -- Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX -- Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction -- RNA Capture-SELEX on Streptavidin Magnetic Beads -- The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE -- Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX -- Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry -- Determination of Aptamer Structure Using Circular Dichroism Spectroscopy -- Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis -- Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance -- Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog -- Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT -- Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments -- Aptasensor for Impedimetric Detection of Lysozyme -- Biophysical Analysis of Small Molecule Binding to Viral RNA Structures -- Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology -- Enzyme-Linked OligoNucleotide Assay (ELONA) -- High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics -- Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis.
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  • 48
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Neurochemistry. ; Enzymology. ; Pharmaceutical chemistry. ; Neurochemistry. ; Enzymology. ; Pharmaceutics.
    In: Springer Nature eBook
    Description / Table of Contents: This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vital resource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.
    Type of Medium: Online Resource
    Pages: X, 254 p. 91 illus., 56 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626436
    Series Statement: Methods in Molecular Biology, 2558
    Language: English
    Note: Purification of MAO A and of MAO B from Mammalian Tissue Sources -- Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System -- The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity -- Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays -- Radiochemical Assay of Monoamine Oxidase Activity -- MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition -- Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase -- Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS) -- Crystallization of Human Monoamine Oxidase B -- Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations -- An (Immune)Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution within the Cell -- Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene-Transfer -- In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers -- In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis -- Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors -- Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology -- Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors.
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  • 49
    Online Resource
    Online Resource
    New York, NY : Springer US
    Subject(s): Cytology. ; Pharmacology. ; Cell Biology. ; Pharmacology.
    In: Springer Nature eBook
    Description / Table of Contents: This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
    Type of Medium: Online Resource
    Pages: XIV, 373 p. 89 illus., 68 illus. in color. , online resource.
    Edition: 1st ed. 2023.
    ISBN: 9781071626719
    Series Statement: Methods in Molecular Biology, 2565
    Language: English
    Note: Rat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.
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  • 50
    Online Resource
    Online Resource
    Cham : Springer International Publishing