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    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: Identification of Glutamatergic Neurons -- Single Nanoparticle Tracking: A Method for Investigating the Surface Dynamics of Glutamate Receptors -- Positron Emission Tomography of Metabotropic Glutamate Receptors -- NMR Spectroscopy of Brain Glutamate Function -- Imaging Glutamate with Genetically Encoded Fluorescent Sensors -- Measurement of Astrocytic Glutamate Release using Genetically-Encoded Probe Combined with TIRF Microscopy -- Drugs to Alter Extracellular Concentration of Glutamate: Modulators of Glutamate Uptake Systems -- Drugs to Tune Up Glutamatergic Systems: Modulators of Glutamate Metabotropic Receptors -- Glutamatergic Synthesis, Recycling, and Receptor Pharmacology at Drosophila and Crustacean Neuromuscular Junctions -- Optical Control of Glutamate Receptors of the NMDA-Kind in Mammalian Neurons, with the use of Photoswitchable Ligands -- In Vivo Electrochemical Studies of Optogenetic-Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays -- Separation-Based Sensors using Microchip Electrophoresis with Microdialysis for Monitoring Glutamate and Other Bioactive Amines -- Brain Glutamate Monitoring by Microdialysis and Separation Methods with a Special Focus on Capillary Electrophoresis with Laser-Induced Fluorescence Detection -- In Vivo Determination of Glutamate Uptake by Brain Microdialysis -- Enzymes of Glutamate System -- The Glutamatergic System as Potential Clinical Biomarkers for Blood and Cerebrospinal Fluid Monitoring -- Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord
    Abstract: This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry
    Pages: XXII, 565 p. 136 illus., 101 illus. in color. : online resource.
    ISBN: 9781493972289
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