Description / Table of Contents:
Subtyping of IPMN -- Clinical Management and Surgical Decision-Making of IPMN of the Pancreas -- Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm -- Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma -- Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar to Ductal Metaplasia -- Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia -- Pancreatic Microtumors: A Novel D Ex Vivo Testing Platform -- 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering -- Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens -- Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma -- An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding -- Osteoclasts Differentiation Assay -- Primary Cultures for Pancreatic Stellate Cells (PSCs) -- Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells -- Quantitative Method to Track Proteolytic Invasion in 3D Collagen -- Detection and Quantification of Macropinosomes in Pancreatic Tumors -- Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo -- Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells -- Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells -- Evaluating the Metabolic Alterations in Pancreatic Cancer -- Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies -- Evaluation of Post-Transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and their mRNA Targets -- Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids -- Statistical Methods for Identifying Biomarkers from miRNA Profiles of Cancers -- Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice -- Pancreatic Acinar to Ductal Metaplasia and Pancreatic Cancer -- Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis -- Molecular and Physiological Evaluation of Pancreatic Cancer-Induced Cachexia
This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have showed to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways to improving patient care for pancreatic cancer
XII, 337 p. 51 illus., 40 illus. in color. : online resource.
3rd ed. 2019.