Description / Table of Contents:
Sustainability in Biobanking- An Introduction to Starting a Biobank -- An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks -- Disaster Prevention and Recovery -- Minority Participation in Biobanks: An Essential Key to Progress -- Orientation and Training of New Biobank Personnel -- Procurement and Storage of Surgical Biospecimens -- Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization -- Procurement, Storage, and Use of Blood in Biobanks -- Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens -- Biobanking of Cerebrospinal Fluid -- Biobanking of Urine Samples -- Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking -- The Fluid Preservation of Zoological Specimens -- Photographing Fluid Preserved Specimens -- Casts of Fluid Preserved Specimens -- Collection and Preservation of Terrestrial Arthropods -- Procurement and Preservation of Plants -- Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank -- Decontamination of Biobank Facilities -- Laboratory Safety: Chemical and Physical Hazards -- Making Formalin-Fixed Paraffin Embedded Blocks -- Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections -- Performing and Cutting Frozen Sections -- An Introduction to the Performance of Immunohistochemistry -- An Introduction to Performing Immunofluorescence Staining -- Making a Tissue Microarray -- Nucleic Acid Isolation and Quality Control -- Fundamentals of RNA Analysis on Biobanked Specimens -- Nucleic Acid Extraction from Human Biological Samples -- An Overview of DNA Analytical Methods -- Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs) -- Sample Preparation for Transmission Electron Microscopy -- Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines -- Domestic and International Shipping of Biospecimens
This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies
XVI, 448 p. 66 illus., 49 illus. in color. : online resource.