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    Keywords: Cytology ; Metabolism ; Cell Biology ; Metabolomics ; Springer eBooks
    Description / Table of Contents: Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
    Abstract: This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field
    Pages: XI, 226 p. 74 illus., 64 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493998319
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